276 lines
33 KiB
XML
276 lines
33 KiB
XML
<document id="89B5C9118A3B97E89AF99F58EEF1BD46" ID-DOI="10.26028/cybium/2021-453-007" ID-ISSN="2101-0315" ID-Zenodo-Dep="10493673" IM.bibliography_approvedBy="julia" IM.illustrations_approvedBy="julia" IM.materialsCitations_approvedBy="julia" IM.tables_approvedBy="julia" IM.taxonomicNames_approvedBy="julia" IM.treatments_approvedBy="julia" checkinTime="1705002796577" checkinUser="felipe" docAuthor="Labbaci, Abdelkader, Chaoui, Lamya, Bahri-Sfar, Lilia, Hammami, Ibtissem & Kara, M. Hichem" docDate="2021" docId="B732B027FFD1A35EFE583E292E32C391" docLanguage="en" docName="Cybium.45.3.225-238.pdf" docOrigin="Cybium 45 (3)" docSource="http://dx.doi.org/10.26028/cybium/2021-453-007" docStyle="DocumentStyle:EAB95795642928CB986F0923DE58F0B7.1:Cybium.2019-.journal_article" docStyleId="EAB95795642928CB986F0923DE58F0B7" docStyleName="Cybium.2019-.journal_article" docStyleVersion="1" docTitle="Aphanius fasciatus" docType="treatment" docVersion="1" lastPageNumber="230" masterDocId="4B0BC85FFFD5A35BFFD23D4D2F04C178" masterDocTitle="Morphogeometric and genetic variations among North African populations of the Mediterranean killifish Aphanius fasciatus (Valenciennes, 1821) from different habitats" masterLastPageNumber="238" masterPageNumber="225" pageNumber="229" updateTime="1712000221125" updateUser="julia" zenodo-license-document="CC-BY-4.0">
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<mods:title id="8C02EE3C4ADDC861C99220482E1C8D41">Morphogeometric and genetic variations among North African populations of the Mediterranean killifish Aphanius fasciatus (Valenciennes, 1821) from different habitats</mods:title>
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<mods:namePart id="7D8058B3C5C24E329301BD55DF1DD74C">Labbaci, Abdelkader</mods:namePart>
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<mods:namePart id="122E7E0BA134FE43BE9231F2A88BDD9A">Chaoui, Lamya</mods:namePart>
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<mods:namePart id="4EED06A7ACF94EE5E25F8A0917E8602E">Bahri-Sfar, Lilia</mods:namePart>
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<mods:namePart id="A86FE2173F1347F87BF0814C60229E5D">Hammami, Ibtissem</mods:namePart>
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<mods:namePart id="22C3B0E0875E77793E63871C0CC8C27F">Kara, M. Hichem</mods:namePart>
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<mods:title id="FDDA373AB8219716BD945B65DA460E9E">Cybium</mods:title>
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<mods:date id="DCE03867886CFEA53F487028713366E6">2021</mods:date>
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<subSubSection id="778152BAFFD1A35FFE583E292D05C204" box="[394,513,868,892]" pageId="4" pageNumber="229" type="nomenclature">
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<paragraph id="3F240131FFD1A35FFE583E292D05C204" blockId="4.[394,513,868,892]" box="[394,513,868,892]" pageId="4" pageNumber="229">
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<heading id="646CB65DFFD1A35FFE583E292D05C204" allCaps="true" bold="true" box="[394,513,868,892]" centered="true" fontSize="9" level="1" pageId="4" pageNumber="229" reason="1">
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<taxonomicName id="F89B7AB2FFD1A35FFE583E292D05C204" ID-CoL="FB5F" authority="(Valenciennes, 1821)" baseAuthorityName="Valenciennes" baseAuthorityYear="1821" box="[394,513,868,892]" family="Cyprinodontidae" genus="Aphanius" kingdom="Animalia" order="Cyprinodontiformes" pageId="4" pageNumber="229" phylum="Chordata" rank="species" species="fasciatus">
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<emphasis id="0DEFDD23FFD1A35FFE583E292D05C204" bold="true" box="[394,513,868,892]" pageId="4" pageNumber="229">RESULTS</emphasis>
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</taxonomicName>
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</heading>
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</paragraph>
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</subSubSection>
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<subSubSection id="778152BAFFD1A35EFF563EE92E32C391" lastPageId="5" lastPageNumber="230" pageId="4" pageNumber="229" type="description">
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<paragraph id="3F240131FFD1A35FFF563EE92E88C2C4" blockId="4.[132,396,932,956]" box="[132,396,932,956]" pageId="4" pageNumber="229">
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<heading id="646CB65DFFD1A35FFF563EE92E88C2C4" bold="true" box="[132,396,932,956]" fontSize="9" level="2" pageId="4" pageNumber="229" reason="3">
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<emphasis id="0DEFDD23FFD1A35FFF563EE92E88C2C4" bold="true" box="[132,396,932,956]" pageId="4" pageNumber="229">Morphological analysis</emphasis>
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</heading>
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</paragraph>
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<paragraph id="3F240131FFD1A35FFF783E882A8DC089" blockId="4.[132,775,965,1511]" lastBlockId="4.[813,1455,217,497]" pageId="4" pageNumber="229">
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Canonical variate analysis (CVA) shows that differences exist between females and males morphology of
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<taxonomicName id="F89B7AB2FFD1A35FFD593EAB2C03C286" baseAuthorityName="Valenciennes" baseAuthorityYear="1821" box="[651,775,998,1022]" family="Cyprinodontidae" genus="Aphanius" kingdom="Animalia" order="Cyprinodontiformes" pageId="4" pageNumber="229" phylum="Chordata" rank="species" species="fasciatus">
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<emphasis id="0DEFDD23FFD1A35FFD593EAB2C03C286" box="[651,775,998,1022]" italics="true" pageId="4" pageNumber="229">A. fasciatus</emphasis>
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</taxonomicName>
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(
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<figureCitation id="A7A01DB4FFD1A35FFF5F394B2FE7C567" box="[141,227,1030,1055]" captionStart="Figure 3" captionStartId="3.[132,195,1220,1242]" captionTargetBox="[138,1437,221,1197]" captionTargetId="figure-345@3.[132,1441,215,1203]" captionTargetPageId="3" captionText="Figure 3. – Projection of Procrustes coordinates and scores of canonical variate on Aphanius fasciatus shape analysis (A) and comparative transformation grids (B). LM: Mellah lagoon, M: Mellah marsh, BZ: Lagoon of Bizerte, LA:Ayata Lake." figureDoi="http://doi.org/10.5281/zenodo.10493681" httpUri="https://zenodo.org/record/10493681/files/figure.png" pageId="4" pageNumber="229">Fig. 3A</figureCitation>
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). These differences are displayed along the second axis, which explains 30.39% of global variance. Specimen shape of Ayata Lake is discriminated from those of the three other localities along the first axis, which represents 45.73% of global variance (
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<figureCitation id="A7A01DB4FFD1A35FFE8039C42EA2C5D9" box="[338,422,1161,1185]" captionStart="Figure 3" captionStartId="3.[132,195,1220,1242]" captionTargetBox="[138,1437,221,1197]" captionTargetId="figure-345@3.[132,1441,215,1203]" captionTargetPageId="3" captionText="Figure 3. – Projection of Procrustes coordinates and scores of canonical variate on Aphanius fasciatus shape analysis (A) and comparative transformation grids (B). LM: Mellah lagoon, M: Mellah marsh, BZ: Lagoon of Bizerte, LA:Ayata Lake." figureDoi="http://doi.org/10.5281/zenodo.10493681" httpUri="https://zenodo.org/record/10493681/files/figure.png" pageId="4" pageNumber="229">Fig. 3A</figureCitation>
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). The variation highlighted along axis 1 is related to landmarks 3, 9, 11, 12 and 14 and 3, 4, 8, 10, 12, 13 for axis 2. For a better appreciation of the shape differences, we expose transformation grids based on procrustes coordinates covariance matrix (
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<figureCitation id="A7A01DB4FFD1A35FFDFB38462D78C45B" box="[553,636,1291,1315]" captionStart="Figure 3" captionStartId="3.[132,195,1220,1242]" captionTargetBox="[138,1437,221,1197]" captionTargetId="figure-345@3.[132,1441,215,1203]" captionTargetPageId="3" captionText="Figure 3. – Projection of Procrustes coordinates and scores of canonical variate on Aphanius fasciatus shape analysis (A) and comparative transformation grids (B). LM: Mellah lagoon, M: Mellah marsh, BZ: Lagoon of Bizerte, LA:Ayata Lake." figureDoi="http://doi.org/10.5281/zenodo.10493681" httpUri="https://zenodo.org/record/10493681/files/figure.png" pageId="4" pageNumber="229">Fig. 3B</figureCitation>
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). Because of the overlapping of Mellah lagoon, Mellah Marsh and
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<collectingRegion id="FD5FCFD3FFD1A35FFD0A38612FABC41C" country="Tunisia" name="Bizerte" pageId="4" pageNumber="229">Bizerte</collectingRegion>
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lagoon specimens, we used discriminate function to obtain a shape pair comparisons female/male. Transformation grids that discriminate sexes within populations were also described (Appendix 1). Based on transformations grids, Ayata Lake fish have a shorter head length than the other populations and the end of the mouth position litters higher (see points 2 and
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figure 3B). The base of opercule seems to be larger for Ayata Lake specimens. We noted variations between sexes in point 9, which corresponds to the insertion of the anal fin (smaller base in Ayata Lake) and more distant from pelvic fin in Ayata Lake. We see a difference between males and females along axis 2 (Cv2) in points 3, 4, 5, 7, 8 and 9. (
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<figureCitation id="A7A01DB4FFD1A35FFBC23CF42B68C0A9" box="[1040,1132,441,465]" captionStart="Figure 3" captionStartId="3.[132,195,1220,1242]" captionTargetBox="[138,1437,221,1197]" captionTargetId="figure-345@3.[132,1441,215,1203]" captionTargetPageId="3" captionText="Figure 3. – Projection of Procrustes coordinates and scores of canonical variate on Aphanius fasciatus shape analysis (A) and comparative transformation grids (B). LM: Mellah lagoon, M: Mellah marsh, BZ: Lagoon of Bizerte, LA:Ayata Lake." figureDoi="http://doi.org/10.5281/zenodo.10493681" httpUri="https://zenodo.org/record/10493681/files/figure.png" pageId="4" pageNumber="229">Fig. 3B</figureCitation>
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). Males are a bit wider than females, from the anal part to the caudal part of the body.
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</paragraph>
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<paragraph id="3F240131FFD1A35FFCFF3F542CE0C349" blockId="4.[813,996,537,561]" box="[813,996,537,561]" pageId="4" pageNumber="229">
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<heading id="646CB65DFFD1A35FFCFF3F542CE0C349" bold="true" box="[813,996,537,561]" fontSize="9" level="2" pageId="4" pageNumber="229" reason="3">
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<emphasis id="0DEFDD23FFD1A35FFCFF3F542CE0C349" bold="true" box="[813,996,537,561]" pageId="4" pageNumber="229">Genetic analysis</emphasis>
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</heading>
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</paragraph>
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<paragraph id="3F240131FFD1A35FFC803F742CD1C269" blockId="4.[813,1456,569,1489]" pageId="4" pageNumber="229">The seven-enzymatic systems (MDH, GOT, PGI, PGM, EST, IDH, LDH) gave clear zymograms. Among fifteen identified loci, eleven were polymorphic (GOT m, GOT f, PGI m, PGI f, PGM-2, EST f1, EST f2, EST m, LDH m, LDH f, IDH f). The mean number of alleles per locus (Am) varies between 1.93 for Ayata Lake and 1.33 for Mellah Marsh (Tab. II).</paragraph>
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<paragraph id="3F240131FFD1A35FFC803E542AA8C569" blockId="4.[813,1456,569,1489]" pageId="4" pageNumber="229">
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The highest value for both allelic richness (Ar = 1.45) and private allele richness (Ap = 0.32) are noted for Ayata Lake sample, while
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<collectingRegion id="FD5FCFD3FFD1A35FFBD63E142B56C209" box="[1028,1106,857,881]" country="Tunisia" name="Bizerte" pageId="4" pageNumber="229">Bizerte</collectingRegion>
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lagoon is nearly close to Mellah lagoon in allelic richness (Ar = 1.21). However, the lowest is observed in Mellah marsh (1.11 for allelic richness and 0.06 for private allele richness) (Tab. II). For the expected heterozygosity, the highest value is
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Ayata Lake and the lowest one in Mellah marsh (He = 0.0294 ± 0.0644).
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</paragraph>
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<paragraph id="3F240131FFD1A35FFC8039542A6BC4A9" blockId="4.[813,1456,569,1489]" pageId="4" pageNumber="229">
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The estimation of Wright fixation index Fis, according to Weir and Cockerham in each sample, revealed a significant deviation from panmixia with Fis = 0.282 (
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<geoCoordinate id="5AAF67F6FFD1A35FFAFD39142A70C509" box="[1327,1396,1113,1137]" degrees="0.505" direction="west" orientation="longitude" pageId="4" pageNumber="229" precision="55" value="-0.505">-0.505</geoCoordinate>
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, 95% CI) in
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<collectingRegion id="FD5FCFD3FFD1A35FFCA339342CBAC5E9" box="[881,958,1145,1169]" country="Tunisia" name="Bizerte" pageId="4" pageNumber="229">Bizerte</collectingRegion>
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lagoon and Fis = 0.524 (
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<geoCoordinate id="5AAF67F6FFD1A35FFAD639342A4FC5E9" box="[1284,1355,1145,1169]" degrees="0.653" direction="west" orientation="longitude" pageId="4" pageNumber="229" precision="55" value="-0.653">-0.653</geoCoordinate>
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, 95% CI) in
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<collectingCounty id="D64579BDFFD1A35FFC9439D42CC5C5C9" box="[838,961,1177,1201]" pageId="4" pageNumber="229">Ayata Lake</collectingCounty>
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. The estimation of global
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<collectingMunicipality id="DF409B4BFFD1A35FFB0039D72BF7C5C9" box="[1234,1267,1178,1201]" pageId="4" pageNumber="229">Fst</collectingMunicipality>
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shows a high significant value (Fst = 0.1246, P = 1).
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<location id="3A4457EAFFD1A35FFB7439F42A11C5A9" LSID="urn:lsid:plazi:treatment:B732B027FFD1A35EFE583E292E32C391:3A4457EAFFD1A35FFB7439F42A11C5A9" box="[1190,1301,1209,1233]" county="Ayata Lake" latitude="0.345" longLatPrecision="78" longitude="-0.653" municipality="Fst" name="Removing" pageId="4" pageNumber="229" stateProvince="Bizerte">Removing</location>
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PGM-2 locus, this value decreases and becomes not significant (Fst = 0.015, P = 0.053).
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<location id="3A4457EAFFD1A35FFC7B39B72CC8C469" LSID="urn:lsid:plazi:treatment:B732B027FFD1A35EFE583E292E32C391:3A4457EAFFD1A35FFC7B39B72CC8C469" box="[937,972,1274,1297]" county="Ayata Lake" latitude="0.345" longLatPrecision="78" longitude="-0.653" municipality="Fst" name="We" pageId="4" pageNumber="229" stateProvince="Bizerte">We</location>
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also note that after a jacknife analysis, when removing
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<location id="3A4457EAFFD1A35FFC4A38542B14C449" LSID="urn:lsid:plazi:treatment:B732B027FFD1A35EFE583E292E32C391:3A4457EAFFD1A35FFC4A38542B14C449" box="[920,1040,1305,1329]" county="Ayata Lake" latitude="0.345" longLatPrecision="78" longitude="-0.653" municipality="Fst" name="Ayata Lake" pageId="4" pageNumber="229" stateProvince="Bizerte">Ayata Lake</location>
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sample, we obtain a lower and not significant value of
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<location id="3A4457EAFFD1A35FFC3738772B03C429" LSID="urn:lsid:plazi:treatment:B732B027FFD1A35EFE583E292E32C391:3A4457EAFFD1A35FFC3738772B03C429" box="[997,1031,1338,1361]" county="Ayata Lake" latitude="0.345" longLatPrecision="78" longitude="-0.653" municipality="Fst" name="Fst" pageId="4" pageNumber="229" stateProvince="Bizerte">Fst</location>
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(0.0064, P = 0.186).
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<location id="3A4457EAFFD1A35FFB3D38742A40C429" LSID="urn:lsid:plazi:treatment:B732B027FFD1A35EFE583E292E32C391:3A4457EAFFD1A35FFB3D38742A40C429" box="[1263,1348,1337,1361]" county="Ayata Lake" latitude="0.345" longLatPrecision="78" longitude="-0.653" municipality="Fst" name="Genetic" pageId="4" pageNumber="229" stateProvince="Bizerte">Genetic</location>
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distances calculated among pairwise samples according to
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<bibRefCitation id="5B0A7CC0FFD1A35FFA9A38142CABC4E9" author="REYNOLDS J. & WEIR B. S. & COCKERHAM C. C." pageId="4" pageNumber="229" pagination="767 - 779" refId="ref8197" refString="REYNOLDS J., WEIR B. S. & COCKERHAM C. C., 1983. - Esti- mation of the coancestry coefficient: basis for a short-term genetic distance. Genetics, 105 (3): 767 - 779." type="journal article" year="1983">
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Reynolds
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<emphasis id="0DEFDD23FFD1A35FFCFF38372C66C4E9" box="[813,866,1401,1425]" italics="true" pageId="4" pageNumber="229">et al.</emphasis>
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||
(1983)
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||
</bibRefCitation>
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showed that the highest distances were between
|
||
<location id="3A4457EAFFD1A35FFCFF38D42CACC4C9" LSID="urn:lsid:plazi:treatment:B732B027FFD1A35EFE583E292E32C391:3A4457EAFFD1A35FFCFF38D42CACC4C9" box="[813,936,1433,1457]" county="Ayata Lake" latitude="0.345" longLatPrecision="78" longitude="-0.653" municipality="Fst" name="Ayata Lake" pageId="4" pageNumber="229" stateProvince="Bizerte">Ayata Lake</location>
|
||
and the three other populations.
|
||
<location id="3A4457EAFFD1A35FFADF38D42A33C4C9" LSID="urn:lsid:plazi:treatment:B732B027FFD1A35EFE583E292E32C391:3A4457EAFFD1A35FFADF38D42A33C4C9" box="[1293,1335,1433,1457]" county="Ayata Lake" latitude="0.345" longLatPrecision="78" longitude="-0.653" municipality="Fst" name="The" pageId="4" pageNumber="229" stateProvince="Bizerte">The</location>
|
||
same finding was observed with
|
||
<location id="3A4457EAFFD1A35FFBF238F72B45C4A9" LSID="urn:lsid:plazi:treatment:B732B027FFD1A35EFE583E292E32C391:3A4457EAFFD1A35FFBF238F72B45C4A9" box="[1056,1089,1466,1489]" county="Ayata Lake" latitude="0.345" longLatPrecision="78" longitude="-0.653" municipality="Fst" name="Fst" pageId="4" pageNumber="229" stateProvince="Bizerte">Fst</location>
|
||
values as shown in table III.
|
||
</paragraph>
|
||
<caption id="6BE451B9FFD1A35FFF563B452FF4C706" pageId="4" pageNumber="229" startId="4.[132,186,1544,1566]" targetBox="[141,968,1677,1898]" targetIsTable="true" targetPageId="4">
|
||
<paragraph id="3F240131FFD1A35FFF563B452FF4C706" blockId="4.[132,942,1544,1662]" pageId="4" pageNumber="229">Table II. – Parameters of genetic diversity in the studied sites. LA: Ayata Lake, BZ: Bizerte Lagoon, LM: Mellah Lagoon, M: Mellah Marsh, Am: Average number of alleles per locus, Ar: Allelic richness, Ap: Private allelic richness, Ho: Observed heterozygosis, He: Expected heterozygosis, Fis: Fixation index; *** P <0.001; ns: not significant.</paragraph>
|
||
</caption>
|
||
<caption id="6BE451B9FFD1A35FFBD73B662B74C7C1" pageId="4" pageNumber="229" startId="4.[1029,1083,1579,1601]" targetBox="[1041,1446,1736,1898]" targetIsTable="true" targetPageId="4" targetType="table">
|
||
<paragraph id="3F240131FFD1A35FFBD73B662B74C7C1" blockId="4.[1029,1456,1579,1721]" pageId="4" pageNumber="229">
|
||
Table III. – Fst values and genetic distances (D) among pairwise populations (Reynolds
|
||
<emphasis id="0DEFDD23FFD1A35FFBD73B162B3CC708" box="[1029,1080,1627,1648]" italics="true" pageId="4" pageNumber="229">et al.</emphasis>
|
||
, 1983). LA: Ayata Lake, BZ: Bizerte Lagoon, LM: Mellah Lagoon, M: Mellah Marsh; *** P <0.001; ** P <0.05; ns: not significant.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="3F240131FFD1A35FFF5F3BC32CB7C612" pageId="4" pageNumber="229">
|
||
<table id="4D9BF391FFD15CA4FF5F3BC02CCCC612" box="[141,968,1677,1898]" gridcols="5" gridrows="7" pageId="4" pageNumber="229">
|
||
<tr id="81AB0373FFD15CA4FF5F3BC02CCCC7DB" box="[141,968,1677,1699]" gridrow="0" pageId="4" pageNumber="229">
|
||
<th id="C27A6A0FFFD15CA4FF5F3BC02FFCC7DB" box="[141,248,1677,1699]" gridcol="0" gridrow="0" pageId="4" pageNumber="229">Parameters</th>
|
||
<th id="C27A6A0FFFD15CA4FED93BC02EACC7DB" box="[267,424,1677,1699]" gridcol="1" gridrow="0" pageId="4" pageNumber="229">LA</th>
|
||
<th id="C27A6A0FFFD15CA4FE123BC02D5AC7DB" box="[448,606,1677,1699]" gridcol="2" gridrow="0" pageId="4" pageNumber="229">BZ</th>
|
||
<th id="C27A6A0FFFD15CA4FDA43BC02C17C7DB" box="[630,787,1677,1699]" gridcol="3" gridrow="0" pageId="4" pageNumber="229">M</th>
|
||
<th id="C27A6A0FFFD15CA4FCF93BC02CCCC7DB" box="[811,968,1677,1699]" gridcol="4" gridrow="0" pageId="4" pageNumber="229">LM</th>
|
||
</tr>
|
||
<tr id="81AB0373FFD15CA4FF5F3BE32CCCC7BC" box="[141,968,1710,1732]" gridrow="1" pageId="4" pageNumber="229">
|
||
<th id="C27A6A0FFFD15CA4FF5F3BE32FFCC7BC" box="[141,248,1710,1732]" gridcol="0" gridrow="1" pageId="4" pageNumber="229">Am</th>
|
||
<td id="C27A6A0FFFD15CA4FED93BE32EACC7BC" box="[267,424,1710,1732]" gridcol="1" gridrow="1" pageId="4" pageNumber="229">1.9333</td>
|
||
<td id="C27A6A0FFFD15CA4FE123BE32D5AC7BC" box="[448,606,1710,1732]" gridcol="2" gridrow="1" pageId="4" pageNumber="229">1.7333</td>
|
||
<td id="C27A6A0FFFD15CA4FDA43BE32C17C7BC" box="[630,787,1710,1732]" gridcol="3" gridrow="1" pageId="4" pageNumber="229">1.3333</td>
|
||
<td id="C27A6A0FFFD15CA4FCF93BE32CCCC7BC" box="[811,968,1710,1732]" gridcol="4" gridrow="1" pageId="4" pageNumber="229">1.5333</td>
|
||
</tr>
|
||
<tr id="81AB0373FFD15CA4FF5F3B9D2CCCC79E" box="[141,968,1744,1766]" gridrow="2" pageId="4" pageNumber="229">
|
||
<th id="C27A6A0FFFD15CA4FF5F3B9D2FFCC79E" box="[141,248,1744,1766]" gridcol="0" gridrow="2" pageId="4" pageNumber="229">Ar</th>
|
||
<td id="C27A6A0FFFD15CA4FED93B9D2EACC79E" box="[267,424,1744,1766]" gridcol="1" gridrow="2" pageId="4" pageNumber="229">1.45</td>
|
||
<td id="C27A6A0FFFD15CA4FE123B9D2D5AC79E" box="[448,606,1744,1766]" gridcol="2" gridrow="2" pageId="4" pageNumber="229">1.21</td>
|
||
<td id="C27A6A0FFFD15CA4FDA43B9D2C17C79E" box="[630,787,1744,1766]" gridcol="3" gridrow="2" pageId="4" pageNumber="229">1.11</td>
|
||
<td id="C27A6A0FFFD15CA4FCF93B9D2CCCC79E" box="[811,968,1744,1766]" gridcol="4" gridrow="2" pageId="4" pageNumber="229">1.15</td>
|
||
</tr>
|
||
<tr id="81AB0373FFD15CA4FF5F3BBC2CCCC67F" box="[141,968,1777,1799]" gridrow="3" pageId="4" pageNumber="229">
|
||
<th id="C27A6A0FFFD15CA4FF5F3BBC2FFCC67F" box="[141,248,1777,1799]" gridcol="0" gridrow="3" pageId="4" pageNumber="229">Ap</th>
|
||
<td id="C27A6A0FFFD15CA4FED93BBC2EACC67F" box="[267,424,1777,1799]" gridcol="1" gridrow="3" pageId="4" pageNumber="229">0.32</td>
|
||
<td id="C27A6A0FFFD15CA4FE123BBC2D5AC67F" box="[448,606,1777,1799]" gridcol="2" gridrow="3" pageId="4" pageNumber="229">0.11</td>
|
||
<td id="C27A6A0FFFD15CA4FDA43BBC2C17C67F" box="[630,787,1777,1799]" gridcol="3" gridrow="3" pageId="4" pageNumber="229">0.06</td>
|
||
<td id="C27A6A0FFFD15CA4FCF93BBC2CCCC67F" box="[811,968,1777,1799]" gridcol="4" gridrow="3" pageId="4" pageNumber="229">0.08</td>
|
||
</tr>
|
||
<tr id="81AB0373FFD15CA4FF5F3A5F2CCCC650" box="[141,968,1810,1832]" gridrow="4" pageId="4" pageNumber="229">
|
||
<th id="C27A6A0FFFD15CA4FF5F3A5F2FFCC650" box="[141,248,1810,1832]" gridcol="0" gridrow="4" pageId="4" pageNumber="229">Ho</th>
|
||
<td id="C27A6A0FFFD15CA4FED93A5F2EACC650" box="[267,424,1810,1832]" gridcol="1" gridrow="4" pageId="4" pageNumber="229">0.0681 ± 0.0977</td>
|
||
<td id="C27A6A0FFFD15CA4FE123A5F2D5AC650" box="[448,606,1810,1832]" gridcol="2" gridrow="4" pageId="4" pageNumber="229">0.0416 ± 0.0681</td>
|
||
<td id="C27A6A0FFFD15CA4FDA43A5F2C17C650" box="[630,787,1810,1832]" gridcol="3" gridrow="4" pageId="4" pageNumber="229">0.0263 ± 0.0542</td>
|
||
<td id="C27A6A0FFFD15CA4FCF93A5F2CCCC650" box="[811,968,1810,1832]" gridcol="4" gridrow="4" pageId="4" pageNumber="229">0.0265 ± 0.0538</td>
|
||
</tr>
|
||
<tr id="81AB0373FFD15CA4FF5F3A7E2CCCC631" box="[141,968,1843,1865]" gridrow="5" pageId="4" pageNumber="229">
|
||
<th id="C27A6A0FFFD15CA4FF5F3A7E2FFCC631" box="[141,248,1843,1865]" gridcol="0" gridrow="5" pageId="4" pageNumber="229">He</th>
|
||
<td id="C27A6A0FFFD15CA4FED93A7E2EACC631" box="[267,424,1843,1865]" gridcol="1" gridrow="5" pageId="4" pageNumber="229">0.1413 ± 0.1803</td>
|
||
<td id="C27A6A0FFFD15CA4FE123A7E2D5AC631" box="[448,606,1843,1865]" gridcol="2" gridrow="5" pageId="4" pageNumber="229">0.0577 ± 0.0904</td>
|
||
<td id="C27A6A0FFFD15CA4FDA43A7E2C17C631" box="[630,787,1843,1865]" gridcol="3" gridrow="5" pageId="4" pageNumber="229">0.0294 ± 0.0644</td>
|
||
<td id="C27A6A0FFFD15CA4FCF93A7E2CCCC631" box="[811,968,1843,1865]" gridcol="4" gridrow="5" pageId="4" pageNumber="229">0.0398 ± 0.0547</td>
|
||
</tr>
|
||
<tr id="81AB0373FFD15CA4FF5F3A192CCCC612" box="[141,968,1876,1898]" gridrow="6" pageId="4" pageNumber="229">
|
||
<th id="C27A6A0FFFD15CA4FF5F3A192FFCC612" box="[141,248,1876,1898]" gridcol="0" gridrow="6" pageId="4" pageNumber="229">Fis</th>
|
||
<td id="C27A6A0FFFD15CA4FED93A192EACC612" box="[267,424,1876,1898]" gridcol="1" gridrow="6" pageId="4" pageNumber="229">0.52371***</td>
|
||
<td id="C27A6A0FFFD15CA4FE123A192D5AC612" box="[448,606,1876,1898]" gridcol="2" gridrow="6" pageId="4" pageNumber="229">0.28201***</td>
|
||
<td id="C27A6A0FFFD15CA4FDA43A192C17C612" box="[630,787,1876,1898]" gridcol="3" gridrow="6" pageId="4" pageNumber="229">0.10817 ns</td>
|
||
<td id="C27A6A0FFFD15CA4FCF93A192CCCC612" box="[811,968,1876,1898]" gridcol="4" gridrow="6" pageId="4" pageNumber="229">0.33669***</td>
|
||
</tr>
|
||
</table>
|
||
</paragraph>
|
||
<paragraph id="3F240131FFD1A35FFBC33B852A38C612" pageId="4" pageNumber="229">
|
||
<table id="4D9BF391FFD15CA4FBC33B852AA2C612" box="[1041,1446,1736,1898]" colsContinueFrom="4.[141,968,1677,1898]" gridcols="5" gridrows="5" pageId="4" pageNumber="229">
|
||
<tr id="81AB0373FFD15CA4FBC33B852AA2C7A5" box="[1041,1446,1736,1757]" gridrow="0" pageId="4" pageNumber="229">
|
||
<th id="C27A6A0FFFD15CA4FBC33B852B41C7A5" box="[1041,1093,1736,1757]" gridcol="0" gridrow="0" pageId="4" pageNumber="229">D Fst</th>
|
||
<th id="C27A6A0FFFD15CA4FB8C3B852B8CC7A5" box="[1118,1160,1736,1757]" gridcol="1" gridrow="0" pageId="4" pageNumber="229">LA</th>
|
||
<th id="C27A6A0FFFD15CA4FB4F3B852BEFC7A5" box="[1181,1259,1736,1757]" gridcol="2" gridrow="0" pageId="4" pageNumber="229">BZ</th>
|
||
<th id="C27A6A0FFFD15CA4FB283B852A4CC7A5" box="[1274,1352,1736,1757]" gridcol="3" gridrow="0" pageId="4" pageNumber="229">M</th>
|
||
<th id="C27A6A0FFFD15CA4FA8A3B852AA2C7A5" box="[1368,1446,1736,1757]" gridcol="4" gridrow="0" pageId="4" pageNumber="229">LM</th>
|
||
</tr>
|
||
<tr id="81AB0373FFD15CA4FBC33BA62AA2C678" box="[1041,1446,1771,1792]" gridrow="1" pageId="4" pageNumber="229" rowspan-1="1">
|
||
<th id="C27A6A0FFFD15CA4FBC33BA62B41C678" box="[1041,1093,1771,1792]" gridcol="0" gridrow="1" pageId="4" pageNumber="229">LA</th>
|
||
<td id="C27A6A0FFFD15CA4FB4F3BA62BEFC678" box="[1181,1259,1771,1792]" gridcol="2" gridrow="1" pageId="4" pageNumber="229">0.19***</td>
|
||
<td id="C27A6A0FFFD15CA4FB283BA62A4CC678" box="[1274,1352,1771,1792]" gridcol="3" gridrow="1" pageId="4" pageNumber="229">0.18***</td>
|
||
<td id="C27A6A0FFFD15CA4FA8A3BA62AA2C678" box="[1368,1446,1771,1792]" gridcol="4" gridrow="1" pageId="4" pageNumber="229">0.20***</td>
|
||
</tr>
|
||
<tr id="81AB0373FFD15CA4FBC33A432AA2C65C" box="[1041,1446,1806,1828]" gridrow="2" pageId="4" pageNumber="229" rowspan-2="1">
|
||
<th id="C27A6A0FFFD15CA4FBC33A432B41C65C" box="[1041,1093,1806,1828]" gridcol="0" gridrow="2" pageId="4" pageNumber="229">BZ</th>
|
||
<td id="C27A6A0FFFD15CA4FB8C3A432B8CC65C" box="[1118,1160,1806,1828]" gridcol="1" gridrow="2" pageId="4" pageNumber="229">0.21</td>
|
||
<td id="C27A6A0FFFD15CA4FB283A432A4CC65C" box="[1274,1352,1806,1828]" gridcol="3" gridrow="2" pageId="4" pageNumber="229">–0.02ns</td>
|
||
<td id="C27A6A0FFFD15CA4FA8A3A432AA2C65C" box="[1368,1446,1806,1828]" gridcol="4" gridrow="2" pageId="4" pageNumber="229">0.03**</td>
|
||
</tr>
|
||
<tr id="81AB0373FFD15CA4FBC33A7C2AA2C63F" box="[1041,1446,1841,1863]" gridrow="3" pageId="4" pageNumber="229" rowspan-3="1">
|
||
<th id="C27A6A0FFFD15CA4FBC33A7C2B41C63F" box="[1041,1093,1841,1863]" gridcol="0" gridrow="3" pageId="4" pageNumber="229">M</th>
|
||
<td id="C27A6A0FFFD15CA4FB8C3A7C2B8CC63F" box="[1118,1160,1841,1863]" gridcol="1" gridrow="3" pageId="4" pageNumber="229">0.19</td>
|
||
<td id="C27A6A0FFFD15CA4FB4F3A7C2BEFC63F" box="[1181,1259,1841,1863]" gridcol="2" gridrow="3" pageId="4" pageNumber="229">–0.02</td>
|
||
<td id="C27A6A0FFFD15CA4FA8A3A7C2AA2C63F" box="[1368,1446,1841,1863]" gridcol="4" gridrow="3" pageId="4" pageNumber="229">–0.01ns</td>
|
||
</tr>
|
||
<tr id="81AB0373FFD15CA4FBC33A182AA2C612" box="[1041,1446,1877,1898]" gridrow="4" pageId="4" pageNumber="229" rowspan-4="1">
|
||
<th id="C27A6A0FFFD15CA4FBC33A182B41C612" box="[1041,1093,1877,1898]" gridcol="0" gridrow="4" pageId="4" pageNumber="229">LM</th>
|
||
<td id="C27A6A0FFFD15CA4FB8C3A182B8CC612" box="[1118,1160,1877,1898]" gridcol="1" gridrow="4" pageId="4" pageNumber="229">0.22</td>
|
||
<td id="C27A6A0FFFD15CA4FB4F3A182BEFC612" box="[1181,1259,1877,1898]" gridcol="2" gridrow="4" pageId="4" pageNumber="229">0.03</td>
|
||
<td id="C27A6A0FFFD15CA4FB283A182A4CC612" box="[1274,1352,1877,1898]" gridcol="3" gridrow="4" pageId="4" pageNumber="229">–0.01</td>
|
||
</tr>
|
||
</table>
|
||
</paragraph>
|
||
<caption id="6BE451B9FFD0A35EFF563CCA2E21C0B5" ID-DOI="http://doi.org/10.5281/zenodo.10493683" ID-Zenodo-Dep="10493683" httpUri="https://zenodo.org/record/10493683/files/figure.png" pageId="5" pageNumber="230" startId="5.[132,197,391,413]" targetBox="[135,774,219,371]" targetPageId="5" targetType="figure">
|
||
<paragraph id="3F240131FFD0A35EFF563CCA2E21C0B5" blockId="5.[132,774,391,461]" pageId="5" pageNumber="230">
|
||
Figure 4. – Neighbour-joining tree on genetic distances D (
|
||
<bibRefCitation id="5B0A7CC0FFD0A35EFD043CCA2E35C0CD" author="REYNOLDS J. & WEIR B. S. & COCKERHAM C. C." pageId="5" pageNumber="230" pagination="767 - 779" refId="ref8197" refString="REYNOLDS J., WEIR B. S. & COCKERHAM C. C., 1983. - Esti- mation of the coancestry coefficient: basis for a short-term genetic distance. Genetics, 105 (3): 767 - 779." type="journal article" year="1983">
|
||
Reynolds
|
||
<emphasis id="0DEFDD23FFD0A35EFF133CED2FF7C0CC" box="[193,243,415,437]" italics="true" pageId="5" pageNumber="230">et al.</emphasis>
|
||
, 1983
|
||
</bibRefCitation>
|
||
) between
|
||
<taxonomicName id="F89B7AB2FFD0A35EFE4A3CD22D0FC0CC" baseAuthorityName="Valenciennes" baseAuthorityYear="1821" box="[408,523,415,436]" family="Cyprinodontidae" genus="Aphanius" kingdom="Animalia" order="Cyprinodontiformes" pageId="5" pageNumber="230" phylum="Chordata" rank="species" species="fasciatus">
|
||
<emphasis id="0DEFDD23FFD0A35EFE4A3CD22D0FC0CC" box="[408,523,415,436]" italics="true" pageId="5" pageNumber="230">A. fasciatus</emphasis>
|
||
</taxonomicName>
|
||
samples from four North African habitats.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="3F240131FFD0A35EFF783CA22E32C391" blockId="5.[132,776,495,745]" pageId="5" pageNumber="230">
|
||
Neighbour-joining tree was drawn basing on genetic distances of Reynold (
|
||
<bibRefCitation id="5B0A7CC0FFD0A35EFE863F422D3EC35F" author="REYNOLDS J. & WEIR B. S. & COCKERHAM C. C." box="[340,570,527,551]" pageId="5" pageNumber="230" pagination="767 - 779" refId="ref8197" refString="REYNOLDS J., WEIR B. S. & COCKERHAM C. C., 1983. - Esti- mation of the coancestry coefficient: basis for a short-term genetic distance. Genetics, 105 (3): 767 - 779." type="journal article" year="1983">
|
||
Reynolds
|
||
<emphasis id="0DEFDD23FFD0A35EFE6D3F5D2EF0C35F" box="[447,500,527,551]" italics="true" pageId="5" pageNumber="230">et al.</emphasis>
|
||
, 1983
|
||
</bibRefCitation>
|
||
). The tree differentiates the populations in two clusters. The first group, supported by a bootstrap value of 100, contains
|
||
<collectingRegion id="FD5FCFD3FFD0A35EFDBA3F1D2DB3C310" box="[616,695,592,616]" country="Tunisia" name="Bizerte" pageId="5" pageNumber="230">Bizerte</collectingRegion>
|
||
lagoon with Mellah lagoon and Mellah marsh as sub clade. This clade shows also high relationships between the two samples of Mellah region. The second clade only includes Ayata lake samples (
|
||
<figureCitation id="A7A01DB4FFD0A35EFF3B3F9C2E2DC391" box="[233,297,721,745]" captionStart="Figure 4" captionStartId="5.[132,197,391,413]" captionTargetBox="[135,774,219,371]" captionTargetId="figure-928@5.[132,775,215,374]" captionTargetPageId="5" captionText="Figure 4. – Neighbour-joining tree on genetic distances D (Reynolds et al., 1983) between A. fasciatus samples from four North African habitats." figureDoi="http://doi.org/10.5281/zenodo.10493683" httpUri="https://zenodo.org/record/10493683/files/figure.png" pageId="5" pageNumber="230">Fig. 4</figureCitation>
|
||
).
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |