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2. Classification and morphology of
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<taxonomicName id="5BABE9A0D001FFE64EF3A410FD5E00EE" ID-CoL="4H47G" authority="L." authorityName="L." box="[507,742,626,650]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="27" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D001FFE64EF3A410FD7F00EE" bold="true" box="[507,711,626,650]" italics="true" pageId="1" pageNumber="27">Phyllanthus niruri</emphasis>
L.
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@ -55,7 +55,7 @@ L.
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<taxonomicName id="5BABE9A0D001FFE64F68A4F2FEFC00C3" box="[96,324,656,680]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="27" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D001FFE64F68A4F2FE9D00CC" box="[96,293,656,680]" italics="true" pageId="1" pageNumber="27">Phyllanthus niruri</emphasis>
L.
</taxonomicName>
@ -82,7 +82,7 @@ it is called Bhumyamalaki
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DPL, in rocky places, damp places such as along waterways, or among grass and shrubs [Dalimarta]. The classification of
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<taxonomicName id="5BABE9A0D001FFE64D10A504FD43011A" box="[536,763,870,894]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="27" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D001FFE64D10A504FD65011A" box="[536,733,870,894]" italics="true" pageId="1" pageNumber="27">Phyllanthus niruri</emphasis>
L.
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@ -107,7 +107,7 @@ L.
[14 Kardinan, 2004]
</paragraph>
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<taxonomicName id="5BABE9A0D001FFE64F68A2B4FEF3068A" box="[96,331,1238,1262]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="1" pageNumber="27" phylum="Chordata" rank="species" species="niruri">
<taxonomicName id="5BABE9A0D001FFE64F68A2B4FEF3068A" box="[96,331,1238,1262]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="27" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D001FFE64F68A2B4FE91068A" box="[96,297,1238,1262]" italics="true" pageId="1" pageNumber="27">Phyllanthus niruri</emphasis>
L.
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@ -135,7 +135,7 @@ is a wild herb plant, originating from Asia and has now reached the African, Aus
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<emphasis id="AEDF4E31D001FFE64E03A1F2FEFF05C1" bold="true" box="[267,327,1936,1957]" pageId="1" pageNumber="27">Fig 1:</emphasis>
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<emphasis id="AEDF4E31D001FFE64E45A1EDFE4505C1" box="[333,509,1935,1957]" italics="true" pageId="1" pageNumber="27">Phyllanthus niruri</emphasis>
L.
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@ -145,7 +145,7 @@ Plant
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<emphasis id="AEDF4E31D001FFE64F68A1A9FD1B0587" bold="true" box="[96,675,1995,2019]" pageId="1" pageNumber="27">
3. The content and benefits of
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<taxonomicName id="5BABE9A0D001FFE64EBFA1A9FD1B0587" box="[439,675,1995,2019]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="27" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D001FFE64EBFA1A9FD3C0587" bold="true" box="[439,644,1995,2019]" italics="true" pageId="1" pageNumber="27">Phyllanthus niruri</emphasis>
L.
</taxonomicName>
@ -153,12 +153,12 @@ L.
</paragraph>
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In general, the
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<taxonomicName id="5BABE9A0D001FFE64E18A18BFE450A65" authority="L. plant" authorityName="L. Plant" box="[272,509,2025,2049]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="27" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D001FFE64E18A18BFE610A65" box="[272,473,2025,2049]" italics="true" pageId="1" pageNumber="27">Phyllanthus niruri</emphasis>
L.
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plant contains several complete chemical compounds, so it is very beneficial for health.
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<taxonomicName id="5BABE9A0D001FFE64FB3AE45FE9B0A5A" box="[187,291,2087,2110]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="27" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D001FFE64FB3AE45FE9B0A5A" box="[187,291,2087,2110]" italics="true" pageId="1" pageNumber="27">P. niruri</emphasis>
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contains alkaloids, flavonoids, tannins, saponins, steroids, phenols and terpenoids
@ -179,7 +179,7 @@ contains alkaloids, flavonoids, tannins, saponins, steroids, phenols and terpeno
</superScript>
. Apart from containing bioactive compounds of flavonoids, tannins, alkaloids, steroids,
<emphasis id="AEDF4E31D001FFE64AB4A681FA6B029E" box="[1468,1491,227,250]" italics="true" pageId="1" pageNumber="27">P.</emphasis>
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<taxonomicName id="5BABE9A0D001FFE64C30A760FCCE037D" box="[824,886,258,281]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="27" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D001FFE64C30A760FCCE037D" box="[824,886,258,281]" italics="true" pageId="1" pageNumber="27">niruri</emphasis>
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also contains molecules of lignans, phyllanthin, hypophyllanthin, glycosides, ellagitannins, triterpenes, phenyl propanoids, ricinolic acid, niruriside, phyltetralin, essential oils, anthraquinones and arbutin
@ -203,7 +203,7 @@ also contains molecules of lignans, phyllanthin, hypophyllanthin, glycosides, el
]
</superScript>
. Quantitatively, the
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plant contains a total phenol equivalent to
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of gallic acid in
@ -213,7 +213,7 @@ of plant extract, while the content of flavonoids in
of plant extract is equivalent to
<quantity id="5B533FC6D001FFE64B3BA430FB3C000F" box="[1075,1156,594,619]" metricMagnitude="-2" metricUnit="kg" metricValue="6.141" pageId="1" pageNumber="27" unit="g" value="61.41">61.41 g</quantity>
of quercetin, while the filantin content in 70%
<taxonomicName id="5BABE9A0D001FFE64CF7A410FBE200ED" box="[1023,1114,625,649]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="1" pageNumber="27" phylum="Chordata" rank="species" species="niruri">P niruri</taxonomicName>
<taxonomicName id="5BABE9A0D001FFE64CF7A410FBE200ED" box="[1023,1114,625,649]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="27" phylum="Tracheophyta" rank="species" species="niruri">P niruri</taxonomicName>
ethanol extract of 0.864%
<superScript id="6BDE3F6BD001FFE64AA7A40DFA750019" attach="right" box="[1455,1485,623,637]" fontSize="6" pageId="1" pageNumber="27">[16]</superScript>
. Traditionally this plant has been widely used by people in tropical countries to treat various
@ -251,7 +251,7 @@ and boosting the immune system
]
</superScript>
. The ability of
<taxonomicName id="5BABE9A0D001FFE64A5BA548FA0B0125" box="[1363,1459,810,833]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="1" pageNumber="27" phylum="Chordata" rank="species" species="niruri">
<taxonomicName id="5BABE9A0D001FFE64A5BA548FA0B0125" box="[1363,1459,810,833]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="27" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D001FFE64A5BA548FA0B0125" box="[1363,1459,810,833]" italics="true" pageId="1" pageNumber="27">P. niruri</emphasis>
</taxonomicName>
to treat various diseases is due to its pharmacological properties, including as an immunomodulator, antiviral, antibacterial, diuretic, anti-hyperglycemic and hepatoprotector
@ -275,7 +275,7 @@ to treat various diseases is due to its pharmacological properties, including as
<paragraph id="9C149223D001FFE64C30A25CFB100611" blockId="1.[824,1492,132,2090]" pageId="1" pageNumber="27">
<emphasis id="AEDF4E31D001FFE64C30A25CFA6B0632" bold="true" box="[824,1491,1086,1110]" pageId="1" pageNumber="27">
4. Applications of
<taxonomicName id="5BABE9A0D001FFE64B6FA25CFA2C0632" box="[1127,1428,1086,1110]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="1" pageNumber="27" phylum="Chordata" rank="species" species="niruri">
<taxonomicName id="5BABE9A0D001FFE64B6FA25CFA2C0632" box="[1127,1428,1086,1110]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="27" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D001FFE64B6FA25CFAEC0632" bold="true" box="[1127,1364,1086,1110]" italics="true" pageId="1" pageNumber="27">Phyllanthus niruri</emphasis>
L.
</taxonomicName>
@ -307,33 +307,33 @@ as an immunostimulant that can increase the resistance of humans and animals inc
Two sets of
<taxonomicName id="5BABE9A0D001FFE64CDCA0E6FB4604F8" box="[980,1278,1668,1692]" family="Cichlidae" genus="Oreochromis" kingdom="Animalia" order="Perciformes" pageId="1" pageNumber="27" phylum="Chordata" rank="species" species="mossambicus">Oreochromis mossambicus</taxonomicName>
fish groups, each consisting of 6 groups (n = 8 per group) were used as test fish to prove the ability of
<taxonomicName id="5BABE9A0D001FFE64B36A0A0FB2604BD" box="[1086,1182,1730,1753]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="1" pageNumber="27" phylum="Chordata" rank="species" species="niruri">
<taxonomicName id="5BABE9A0D001FFE64B36A0A0FB2604BD" box="[1086,1182,1730,1753]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="27" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D001FFE64B36A0A0FB2604BD" box="[1086,1182,1730,1753]" italics="true" pageId="1" pageNumber="27">P. niruri</emphasis>
</taxonomicName>
as an immunostimulant by observing the levels of an body titers and neutrophil levels. The part of the
<taxonomicName id="5BABE9A0D001FFE64CD6A09DFB820572" box="[990,1082,1791,1814]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="1" pageNumber="27" phylum="Chordata" rank="species" species="niruri">
<taxonomicName id="5BABE9A0D001FFE64CD6A09DFB820572" box="[990,1082,1791,1814]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="27" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D001FFE64CD6A09DFB820572" box="[990,1082,1791,1814]" italics="true" pageId="1" pageNumber="27">P. niruri</emphasis>
</taxonomicName>
plant used was the leaf in the form of an aqueous extract. The extract was given to fish by intraperitoneal injection with various doses, namely 20, 2, 0.02 and
<quantity id="5B533FC6D001FFE64CA4A138FBA00516" box="[940,1048,1882,1906]" metricMagnitude="-9" metricUnit="kg" metricValue="2.0" pageId="1" pageNumber="27" unit="mg" value="0.002">0.002 mg</quantity>
/kg fish body weight. To observe the activity of antibody titers, the first group of fish, after two days of administration of
<taxonomicName id="5BABE9A0D001FFE64B89A1FBFB5305CB" box="[1153,1259,1944,1968]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="1" pageNumber="27" phylum="Chordata" rank="species" species="niruri">
<taxonomicName id="5BABE9A0D001FFE64B89A1FBFB5305CB" box="[1153,1259,1944,1968]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="27" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D001FFE64B89A1FBFB5305CB" box="[1153,1259,1944,1968]" italics="true" pageId="1" pageNumber="27">P. niruri</emphasis>
</taxonomicName>
leaf extract, were challenged with Sheep red blood cells (SRBC) which function as antigens. Sheep red blood cells (SRBC) are given by intraperitoneal injection with the first dose of 5% of 0.1 mL and a second dose of 25% (booster) of 0.1 mL given three days after the first dose, then observed for 60 days from the first injection. As for observing neutrophil activity, fish in the second set were challenged with 100 μL HA- BSA (heat aggregated-bovine serum albumin) with a concentration of
<quantity id="5B533FC6D002FFE54DE5A680FF3A037E" metricMagnitude="-6" metricUnit="kg" metricValue="5.0" pageId="2" pageNumber="28" unit="mg" value="5.0">5 mg</quantity>
via intraperitoneal injection, then observations were made for 12 days. The results showed that administration of
<taxonomicName id="5BABE9A0D002FFE54DECA743FF260332" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="28" phylum="Chordata" rank="species" species="niruri">
<taxonomicName id="5BABE9A0D002FFE54DECA743FF260332" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D002FFE54DECA743FF260332" italics="true" pageId="2" pageNumber="28">P. niruri</emphasis>
</taxonomicName>
aqueous extract resulted in a significant increase compared to controls in the activation of antibody and neutrophil responses. Of the various doses tested, the group of fish that were given
<taxonomicName id="5BABE9A0D002FFE54E34A7F9FE2103D6" box="[316,409,411,434]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="28" phylum="Chordata" rank="species" species="niruri">
<taxonomicName id="5BABE9A0D002FFE54E34A7F9FE2103D6" box="[316,409,411,434]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D002FFE54E34A7F9FE2103D6" box="[316,409,411,434]" italics="true" pageId="2" pageNumber="28">P. niruri</emphasis>
</taxonomicName>
leaf aqueous extract at a dose of
<quantity id="5B533FC6D002FFE54F68A7DBFF1B03B5" box="[96,163,441,465]" metricMagnitude="-5" metricUnit="kg" metricValue="2.0" pageId="2" pageNumber="28" unit="mg" value="20.0">20 mg</quantity>
/kg body weight gave the maximum increase in primary and secondary antibody responses on the 15th day of observation. Whereas the group of fish that were given
<taxonomicName id="5BABE9A0D002FFE54DEDA795FF230049" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="28" phylum="Chordata" rank="species" species="niruri">P. niruri</taxonomicName>
<taxonomicName id="5BABE9A0D002FFE54DEDA795FF230049" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niruri">P. niruri</taxonomicName>
leaf aqueous extract at a dose of
<quantity id="5B533FC6D002FFE54D3FA477FD2C0049" box="[567,660,533,557]" metricMagnitude="-8" metricUnit="kg" metricValue="2.0" pageId="2" pageNumber="28" unit="mg" value="0.02">0.02 mg</quantity>
/kg body weight resulted in a maximum increase in neutrophil levels on day 6
@ -347,7 +347,7 @@ leaf aqueous extract at a dose of
<emphasis id="AEDF4E31D002FFE54E54A430FDF5000E" box="[348,589,594,618]" italics="true" pageId="2" pageNumber="28">Oreochromis niloticus</emphasis>
</taxonomicName>
) that have been given
<taxonomicName id="5BABE9A0D002FFE54FA2A410FEB600ED" box="[170,270,626,649]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="28" phylum="Chordata" rank="species" species="niruri">
<taxonomicName id="5BABE9A0D002FFE54FA2A410FEB600ED" box="[170,270,626,649]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D002FFE54FA2A410FEB600ED" box="[170,270,626,649]" italics="true" pageId="2" pageNumber="28">P. niruri</emphasis>
</taxonomicName>
leaf extract through injection are able to survive against
@ -361,13 +361,13 @@ was significantly the best concentration with the highest survival of tilapia, w
<emphasis id="AEDF4E31D002FFE54B44A6A6FB4702BF" box="[1100,1279,196,219]" italics="true" pageId="2" pageNumber="28">Cyprinus carpio</emphasis>
</taxonomicName>
) after being given feed mixed with
<taxonomicName id="5BABE9A0D002FFE54B0DA681FBDD029E" box="[1029,1125,226,250]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="28" phylum="Chordata" rank="species" species="niruri">P. niruri</taxonomicName>
<taxonomicName id="5BABE9A0D002FFE54B0DA681FBDD029E" box="[1029,1125,226,250]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niruri">P. niruri</taxonomicName>
powder for 60 days was then challenged with
<taxonomicName id="5BABE9A0D002FFE54CE6A763FB3D037D" authorityName="Stanier" authorityYear="1943" baseAuthorityName="Chester" baseAuthorityYear="1901" box="[1006,1157,257,281]" class="Gammaproteobacteria" family="Aeromonadaceae" genus="Aeromonas" kingdom="Bacteria" order="Aeromonadales" pageId="2" pageNumber="28" phylum="Proteobacteria" rank="species" species="hydrophila">A. hydrophila</taxonomicName>
bacteria with a density of
<superScript id="6BDE3F6BD002FFE54AA6A763FA6C0369" attach="right" box="[1454,1492,255,281]" fontSize="6" pageId="2" pageNumber="28">103</superScript>
CFU with the cohabitation method through water in a rearing aquarium, able to survive the attack of these bacteria. Giving
<taxonomicName id="5BABE9A0D002FFE54C30A73CFC290311" box="[824,913,349,373]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="28" phylum="Chordata" rank="species" species="niluri">
<taxonomicName id="5BABE9A0D002FFE54C30A73CFC290311" box="[824,913,349,373]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niluri">
<emphasis id="AEDF4E31D002FFE54C30A73CFC290311" box="[824,913,349,373]" italics="true" pageId="2" pageNumber="28">P. niluri</emphasis>
</taxonomicName>
powder as much as 2% (
@ -398,13 +398,13 @@ The results of research conducted by Ma'at
]
</superScript>
showed that
<taxonomicName id="5BABE9A0D002FFE54CC3A7BBFB950394" box="[971,1069,473,496]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="28" phylum="Chordata" rank="species" species="niruri">
<taxonomicName id="5BABE9A0D002FFE54CC3A7BBFB950394" box="[971,1069,473,496]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D002FFE54CC3A7BBFB950394" box="[971,1069,473,496]" italics="true" pageId="2" pageNumber="28">P. niruri</emphasis>
</taxonomicName>
had an immunomodulating effect
<emphasis id="AEDF4E31D002FFE54AB6A7BBFCD2006A" italics="true" pageId="2" pageNumber="28">in vitro</emphasis>
.
<taxonomicName id="5BABE9A0D002FFE54C72A795FC6B006A" box="[890,979,502,526]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="28" phylum="Chordata" rank="species" species="niruri">P. niruri</taxonomicName>
<taxonomicName id="5BABE9A0D002FFE54C72A795FC6B006A" box="[890,979,502,526]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niruri">P. niruri</taxonomicName>
extract (
<quantity id="5B533FC6D002FFE54B3CA794FB14006A" box="[1076,1196,502,526]" metricMagnitude="-4" metricUnit="kg" metricValue="1.25" metricValueMax="2.0" metricValueMin="0.5" pageId="2" pageNumber="28" unit="mg" value="125.0" valueMax="200.0" valueMin="50.0">50-200 mg</quantity>
/kg) can elicit both cellular and humoral immune responses. As the results of research by Nworu
@ -418,7 +418,7 @@ extract (
showed
<emphasis id="AEDF4E31D002FFE54BA1A451FA83002F" box="[1193,1339,563,587]" italics="true" pageId="2" pageNumber="28">
that
<taxonomicName id="5BABE9A0D002FFE54BD4A456FA83002F" box="[1244,1339,564,587]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="28" phylum="Chordata" rank="species" species="niruri">P. niruri</taxonomicName>
<taxonomicName id="5BABE9A0D002FFE54BD4A456FA83002F" box="[1244,1339,564,587]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niruri">P. niruri</taxonomicName>
</emphasis>
water extract (
<quantity id="5B533FC6D002FFE54C48A430FC00000E" box="[832,952,594,618]" metricMagnitude="-1" metricUnit="kg" metricValue="1.0625" metricValueMax="2.0" metricValueMin="0.125" pageId="2" pageNumber="28" unit="g" value="106.25" valueMax="200.0" valueMin="12.5">12.5-200 g</quantity>
@ -428,7 +428,7 @@ water extract (
<paragraph id="9C149223D002FFE54EB0A4AFFBC30086" blockId="2.[440,1147,716,738]" box="[440,1147,716,738]" pageId="2" pageNumber="28">
<emphasis id="AEDF4E31D002FFE54EB0A4AFFDB40086" bold="true" box="[440,524,717,738]" pageId="2" pageNumber="28">Table 1:</emphasis>
Applications of
<taxonomicName id="5BABE9A0D002FFE54DA5A4AEFBC30086" authority="L. Sebagai Immunostimulant" authorityName="L. Sebagai Immunostimulant" box="[685,1147,716,738]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="28" phylum="Chordata" rank="species" species="niruri">
<taxonomicName id="5BABE9A0D002FFE54DA5A4AEFBC30086" authority="L. Sebagai Immunostimulant" authorityName="L. Sebagai Immunostimulant" box="[685,1147,716,738]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D002FFE54DA5A4AEFCE60086" box="[685,862,716,738]" italics="true" pageId="2" pageNumber="28">Phyllanthus niruri</emphasis>
L. Sebagai Immunostimulant
</taxonomicName>
@ -461,14 +461,14 @@ L. Sebagai Immunostimulant
</th>
<td id="614AF91DD00200184E10A574FE54011A" box="[280,492,790,894]" gridcol="1" gridrow="1" pageId="2" pageNumber="28">
Administration of
<taxonomicName id="5BABE9A0D002FFE54ED8A546FEE80131" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="28" phylum="Chordata" rank="species" species="niruri">
<taxonomicName id="5BABE9A0D002FFE54ED8A546FEE80131" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D002FFE54ED8A546FEE80131" italics="true" pageId="2" pageNumber="28">P. niruri</emphasis>
</taxonomicName>
aqueous extract via intraperitoneal.
</td>
<td id="614AF91DD00200184D01A574FC48011A" box="[521,1008,790,894]" gridcol="2" gridrow="1" pageId="2" pageNumber="28">Injection Fish were injected intraperitoneally with Sheep red blood cells (SRBC). The first dose of 0.1 mL of 5% and the second dose (booster) of 0.1 mL of 25%</td>
<td id="614AF91DD00200184B19A574FACC011A" box="[1041,1396,790,894]" gridcol="3" gridrow="1" pageId="2" pageNumber="28">
<taxonomicName id="5BABE9A0D002FFE54B11A575FBD30148" box="[1049,1131,791,812]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="28" phylum="Chordata" rank="species" species="niruri">
<taxonomicName id="5BABE9A0D002FFE54B11A575FBD30148" box="[1049,1131,791,812]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D002FFE54B11A575FBD30148" box="[1049,1131,791,812]" italics="true" pageId="2" pageNumber="28">P. niruri</emphasis>
</taxonomicName>
extract dose of 20 mg/fish body weight resulted in a maximum increase in antibody levels on day 15.
@ -487,7 +487,7 @@ extract dose of 20 mg/fish body weight resulted in a maximum increase in antibod
</th>
<td id="614AF91DD00200184E10A5E7FE54066E" box="[280,492,901,1034]" gridcol="1" gridrow="2" pageId="2" pageNumber="28">
Administration of
<taxonomicName id="5BABE9A0D002FFE54ED8A5E4FEE701D3" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="28" phylum="Chordata" rank="species" species="niruri">
<taxonomicName id="5BABE9A0D002FFE54ED8A5E4FEE701D3" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D002FFE54ED8A5E4FEE701D3" italics="true" pageId="2" pageNumber="28">P. niruri</emphasis>
</taxonomicName>
leaf aqueous extract by intraperitoneal injection.
@ -495,7 +495,7 @@ leaf aqueous extract by intraperitoneal injection.
<td id="614AF91DD00200184D01A5E7FC48066E" box="[521,1008,901,1034]" gridcol="2" gridrow="2" pageId="2" pageNumber="28">Fish were injected intraperitoneally with HA-BSA (heat aggregated-bovine serum albumin) with a concentration of 5 mg as much as 100 μL.</td>
<td id="614AF91DD00200184B19A5E7FACC066E" box="[1041,1396,901,1034]" gridcol="3" gridrow="2" pageId="2" pageNumber="28">
A dose of
<taxonomicName id="5BABE9A0D002FFE54B8CA5F6FB6A01CD" box="[1156,1234,915,937]" class="Aves" family="Leiothrichidae" genus="Phyllanthus" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="28" phylum="Chordata" rank="species" species="niruri">P. niruri</taxonomicName>
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extract of 0.02 mg/kg body weight resulted in a maximum increase in neutrophil levels on the 6th day.
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@ -512,7 +512,7 @@ extract of 0.02 mg/kg body weight resulted in a maximum increase in neutrophil l
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Administration of
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<taxonomicName id="5BABE9A0D002FFE54ED8A270FEE70626" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D002FFE54ED8A270FEE70626" italics="true" pageId="2" pageNumber="28">P. niruri</emphasis>
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leaf aqueous extract by injection.
@ -539,7 +539,7 @@ bacteria.
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Giving water extract of
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leaves through feed.
@ -564,7 +564,7 @@ Infection of fish with
In
<tableCitation id="D129A798D002FFE54F88A2BAFF6C0694" box="[128,212,1240,1264]" captionStart="Table 1" captionStartId="2.[440,498,717,738]" captionTargetId="graphics-403@2.[108,1479,754,1203]" captionTargetPageId="2" captionText="Table 1: Applications of Phyllanthus niruri L. Sebagai Immunostimulant" httpUri="http://table.plazi.org/id/C8D4C2ABD002FFE54EB0A4AFFBC30086" pageId="2" pageNumber="28" tableUuid="C8D4C2ABD002FFE54EB0A4AFFBC30086">Table 1</tableCitation>
it can be seen that the
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plant used as an immunostimulant is the leaf part, in the form of extract or powder (flour) and the method of administration is also by injection or through feed. Testing the ability of these plants as immunostimulants in fish can be carried out using various challenge test methods and various observational indicators, among others by looking at antibody levels, neurophil levels and survival of fish after being challenged with pathogenic bacteria. The
@ -573,7 +573,7 @@ of fish tested, the challenge test method and the dosage used influenced the res
</paragraph>
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Based on the results of several studies above, it proves that
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<emphasis id="AEDF4E31D002FFE54DECA06EFF260425" italics="true" pageId="2" pageNumber="28">P. niruri</emphasis>
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can be used as an immunomodulator that can induce fish resistance to pathogenic bacteria. As according to the POM Agency (2006)
@ -583,33 +583,33 @@ can be used as an immunomodulator that can induce fish resistance to pathogenic
]
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that
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<emphasis id="AEDF4E31D002FFE54D12A00AFD37041B" box="[538,655,1640,1663]" italics="true" pageId="2" pageNumber="28">P. niruri</emphasis>
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is an immunomodulator, which can improve or enhance the immune system. The main component and is an active compound as an immunostimulant which is contained in
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<taxonomicName id="5BABE9A0D002FFE54DE2A0A6FF26049D" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niruri">
<emphasis id="AEDF4E31D002FFE54DE2A0A6FF26049D" italics="true" pageId="2" pageNumber="28">P niruri</emphasis>
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flavonoids. The mechanism of flavonoids as an immunostimulant is to activate the body's cells by sending intracellular signals to cell receptors, so that the body's cells work optimally. Apart from flavonoids, other compounds contained in
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, including phyllanthin and hyphophyllanthin are two compounds that have antiinflammatory activity so that they can strengthen immunity. Another opinion states that
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has an effect as an immunomodulator through activation and augmentation of the cellular immune system. Specifically
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can activate neutrophils, macrophages, monocytes, T and B lymphocytes. The increase in phagocytosis by neutrophils indicates an accelerated process of eradicating pathogenic microbes, especially for extracellular pathogens, such as viruses, bacteria or fungi.
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can induce an increase in monocytes and macrophages, which are able to lyse infecting intracellular pathogenic cells and expose these pathogenic cells to other immune components in the extracellular compartment. In addition,
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can modulate cytokine secretion, including stimulating IFN-γ, TNF- α, IL-4, IL-6, IL-12 and suppression of IL-10. This shows that
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<taxonomicName id="5BABE9A0D002FFE54A4BA38FFA1A0460" box="[1347,1442,1517,1540]" class="Magnoliopsida" family="Phyllanthaceae" genus="Phyllanthus" kingdom="Plantae" order="Malpighiales" pageId="2" pageNumber="28" phylum="Tracheophyta" rank="species" species="niruri">
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can induce the body's defense, by engaging the cellular immune system against foreign pathogens
@ -619,7 +619,7 @@ can induce the body's defense, by engaging the cellular immune system against fo
]
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. On the basis of the immunostimulatory properties possessed by
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<emphasis id="AEDF4E31D002FFE54A28A02BFAC70404" box="[1312,1407,1609,1632]" italics="true" pageId="2" pageNumber="28">P. niruri</emphasis>
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from a series of non-specific (neutrophil), specific (antibody) parameter tests and other tests, namely the survival rate of fish challenged with pathogenic bacteria can be used either as a routine feed supplement to activate the immune system of cultivated fish, other than that it can be used as an adjuvant in the use of vaccines.

View file

@ -1,67 +1,67 @@
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