161 lines
18 KiB
XML
161 lines
18 KiB
XML
<document id="E80B92769CB6EED28E99E1856AF5110D" ID-CLB-Dataset="298642" ID-DOI="10.33910/2686-9519-2023-15-4-712-723" ID-GBIF-Dataset="8d14e039-7ef9-4095-9397-e40671140cf4" ID-ISSN="add_ISSN_here" ID-Zenodo-Dep="11362243" ID-ZooBank="7EDFB492-33F5-4F6F-BB32-FEC4BB86098F" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="guilherme" IM.taxonomicNames_approvedBy="guilherme" IM.treatments_approvedBy="guilherme" checkinTime="1716858179615" checkinUser="felipe" docAuthor="Ataev, Gennady L." docDate="2023" docId="03E087B9682BFFC227F7FAF48F286CE7" docLanguage="en" docName="AmurianZoolJ.XV.4.712-723.pdf" docOrigin="Amurian Zoological Journal XV (4)" docSource="http://dx.doi.org/10.33910/2686-9519-2023-15-4-712-723" docStyle="DocumentStyle:FCE553B9173680C72BD6FA2C2AEC2C2C.2:AmurianZoolJ.2019-.journal_article.russian" docStyleId="FCE553B9173680C72BD6FA2C2AEC2C2C" docStyleName="AmurianZoolJ.2019-.journal_article.russian" docStyleVersion="2" docTitle="Biomphalaria pfeifferi" docType="treatment" docVersion="6" lastPageNumber="716" masterDocId="FFD9FFC16829FFC6247FFFBA8A5F6D7B" masterDocTitle="ЭкспериментаΛьное изучение вΛияния метацеркарий Echinostoma caproni на выживаемость моΛΛюсков Biomphalaria pfeifferi" masterLastPageNumber="723" masterPageNumber="712" pageNumber="714" updateTime="1718739848650" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-NC-4.0">
|
||
<mods:mods id="F78ACB31273ABA7834C52A5D59260390" xmlns:mods="http://www.loc.gov/mods/v3">
|
||
<mods:titleInfo id="7CAA976A9EAC84EC2B2F6B70E4113D1E">
|
||
<mods:title id="BA4FAFAF4C7A55B4B32D1BA68D0370DE">ЭкспериментаΛьное изучение вΛияния метацеркарий Echinostoma caproni на выживаемость моΛΛюсков Biomphalaria pfeifferi</mods:title>
|
||
</mods:titleInfo>
|
||
<mods:name id="58EFD672C806F11147E5D7B18147ADF8" type="personal">
|
||
<mods:role id="0AA31FF45C0F97A553D0E484EC51877B">
|
||
<mods:roleTerm id="FAF77CC9D1C0A6C76C76DC7991EB72D0">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="15779BE692B71EB19C7DA2C733D05986">Ataev, Gennady L.</mods:namePart>
|
||
</mods:name>
|
||
<mods:typeOfResource id="DA2B10A13979DCC77449C4419050786C">text</mods:typeOfResource>
|
||
<mods:relatedItem id="6B69280F561E09A891D19F7619DF2B28" type="host">
|
||
<mods:titleInfo id="BCC200A96334D3DA107A09AAE1BA30DC">
|
||
<mods:title id="4EE54621A5171125D9FBA94C91FB4B77">Amurian Zoological Journal</mods:title>
|
||
</mods:titleInfo>
|
||
<mods:part id="B0C36ACFF445CA0721AEE9D1BDBCD8C5">
|
||
<mods:date id="124C572C417303C86BD3402540342969">2023</mods:date>
|
||
<mods:detail id="91904F2FF859571662A17215DDEFE73F" type="pubDate">
|
||
<mods:number id="8F223C8F44840241632CB65EDD74D1E2">2023-12-28</mods:number>
|
||
</mods:detail>
|
||
<mods:detail id="42F8A43CA1A72F671C7FCE51D717754D" type="volume">
|
||
<mods:number id="FF3D4C940F371835D36DAD298DB643D1">XV</mods:number>
|
||
</mods:detail>
|
||
<mods:detail id="0AC0011AB5458A1ADDFDDC8E3CA7A9E0" type="issue">
|
||
<mods:number id="C0F7EFC6C6E40EF0B16E90DB691B475D">4</mods:number>
|
||
</mods:detail>
|
||
<mods:extent id="B1C1D329F49FA7936E394B26EFAB7776" unit="page">
|
||
<mods:start id="CF667BF9CFB196EC2D97FA24B8E8B533">712</mods:start>
|
||
<mods:end id="557A53CC1514354A745CC65A3A133849">723</mods:end>
|
||
</mods:extent>
|
||
</mods:part>
|
||
</mods:relatedItem>
|
||
<mods:location id="F79A2208F5A0253317451945A9615AD7">
|
||
<mods:url id="1336B35D16B68BF08CFFFA5E9766F612">http://dx.doi.org/10.33910/2686-9519-2023-15-4-712-723</mods:url>
|
||
</mods:location>
|
||
<mods:classification id="13D903433547218A227790317191E918">journal article</mods:classification>
|
||
<mods:identifier id="F5A52E570B2D04EEC6586ED84BD76A63" type="CLB-Dataset">298642</mods:identifier>
|
||
<mods:identifier id="D5E6C393551F427A28B160A831ED9992" type="DOI">10.33910/2686-9519-2023-15-4-712-723</mods:identifier>
|
||
<mods:identifier id="CE0701B49DC0E42EBC2880D32ABB048F" type="GBIF-Dataset">8d14e039-7ef9-4095-9397-e40671140cf4</mods:identifier>
|
||
<mods:identifier id="E9442D55C0843428BE38D3480ADE783E" type="ISSN">add_ISSN_here</mods:identifier>
|
||
<mods:identifier id="E1D67382ED809345CC6B00282D6A822F" type="Zenodo-Dep">11362243</mods:identifier>
|
||
<mods:identifier id="7688BBE3554DE700B47D7C882C387E21" type="ZooBank">7EDFB492-33F5-4F6F-BB32-FEC4BB86098F</mods:identifier>
|
||
</mods:mods>
|
||
<treatment id="03E087B9682BFFC227F7FAF48F286CE7" ID-DOI="http://doi.org/10.5281/zenodo.12110258" ID-GBIF-Taxon="233223865" ID-Zenodo-Dep="12110258" LSID="urn:lsid:plazi:treatment:03E087B9682BFFC227F7FAF48F286CE7" httpUri="http://treatment.plazi.org/id/03E087B9682BFFC227F7FAF48F286CE7" lastPageId="4" lastPageNumber="716" pageId="2" pageNumber="714">
|
||
<subSubSection id="C3536524682BFFC427F7FAF48F7E68EA" pageId="2" pageNumber="714" type="nomenclature">
|
||
<paragraph id="8BF636AF682BFFC427F7FAF48F7E68EA" blockId="2.[879,1313,1358,1425]" pageId="2" pageNumber="714">
|
||
<heading id="D0BE81C3682BFFC427F7FAF48F7E68EA" centered="true" fontSize="12" level="2" pageId="2" pageNumber="714" reason="9">
|
||
<emphasis id="B93DEABD682BFFC427F7FAF48F7E68EA" bold="true" italics="true" pageId="2" pageNumber="714">
|
||
Mortality of
|
||
<taxonomicName id="4C494D2C682BFFC42046FAF58E916810" ID-CoL="LV3X" authority="(Krauss, 1848)" baseAuthorityName="Krauss" baseAuthorityYear="1848" box="[1081,1230,1358,1387]" class="Gastropoda" family="Planorbidae" genus="Biomphalaria" kingdom="Animalia" order="Littorinimorpha" pageId="2" pageNumber="714" phylum="Mollusca" rank="species" species="pfeifferi">B. pfeifferi</taxonomicName>
|
||
due to metacercariae autoinfection
|
||
</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C3536524682BFFC2273FFA118F286CE7" lastPageId="4" lastPageNumber="716" pageId="2" pageNumber="714" type="description">
|
||
<paragraph id="8BF636AF682BFFC4273FFA1189B36BA2" blockId="2.[794,1399,1451,2027]" pageId="2" pageNumber="714">
|
||
The first deaths of
|
||
<taxonomicName id="4C494D2C682BFFC42038FA168E8C68BC" baseAuthorityName="Krauss" baseAuthorityYear="1848" box="[1095,1235,1451,1480]" class="Gastropoda" family="Planorbidae" genus="Biomphalaria" kingdom="Animalia" order="Littorinimorpha" pageId="2" pageNumber="714" phylum="Mollusca" rank="species" species="pfeifferi">
|
||
<emphasis id="B93DEABD682BFFC42038FA168E8C68BC" box="[1095,1235,1451,1480]" italics="true" pageId="2" pageNumber="714">B. pfeifferi</emphasis>
|
||
</taxonomicName>
|
||
snails from Group 1 were registered 3 weeks
|
||
<collectionCode id="ED58AE6A682BFFC42092FA698F526894" box="[1261,1293,1491,1519]" country="Russia" name="Paleontological Institute" pageId="2" pageNumber="714">PI</collectionCode>
|
||
. This is when the release of cercariae first began. More than half of the snails died a month
|
||
<collectionCode id="ED58AE6A682BFFC42090F99B8F536B46" box="[1263,1292,1569,1597]" country="Russia" name="Paleontological Institute" pageId="2" pageNumber="714">PI</collectionCode>
|
||
(
|
||
<figureCitation id="13722A2A682BFFC42164F99A8F386B46" box="[1307,1383,1568,1597]" captionStart="Fig" captionStartId="4.[163,210,1814,1843]" captionTargetBox="[191,1359,1168,1798]" captionTargetId="figure-429@4.[191,1359,1168,1805]" captionTargetPageId="4" captionText="Fig. 2. Dynamics of mortality of Biomphalaria pfeifferi molluscs. Group 1* — red line / 38 days PI; Group 2* — green line / 56 days PI; Group 3* — blue line / 60 days PI; Group 4 — lilac line (magenta) / 58 days PI Рис. 2. Àинамика смертности моΛΛюсков Biomphalaria pfeifferi. Серия 1* — красная Λиния /38 Δней P. I.; Серия 2* — зеΛёная Λиния / 56 Δней P. I.; Серия 3* — гоΛубая Λиния / 60 Δней P. I.; Серия 4 — ΛиΛовая Λиния (magenta) / 58 Δней P. I." figureDoi="http://doi.org/10.5281/zenodo.11362249" httpUri="https://zenodo.org/record/11362249/files/figure.png" pageId="2" pageNumber="714">Fig. 2</figureCitation>
|
||
). The last snail from Group 1 died 38 days
|
||
<collectionCode id="ED58AE6A682BFFC4212DF9F28F2D6B1F" box="[1362,1394,1608,1636]" country="Russia" name="Paleontological Institute" pageId="2" pageNumber="714">PI</collectionCode>
|
||
. By contrast, only 3 uninfected snails (6%) from the control group (Group 5) died during the experiment.
|
||
</paragraph>
|
||
<paragraph id="8BF636AF682BFFC4273FF95E89FA6A90" blockId="2.[794,1399,1451,2027]" pageId="2" pageNumber="714">As we mentioned earlier, the density of cercariae in the aquaria of Groups 2 and 4 was lowered artificially by either frequent filtration of water (Group 2) or by using uninfected snails (Group 4) as a biological cercarial filter in the aquarium with infected snails (Group 3).</paragraph>
|
||
<caption id="DF366627682AFFC524DFF9F48E3B6AB9" ID-DOI="http://doi.org/10.5281/zenodo.11362245" ID-Zenodo-Dep="11362245" httpUri="https://zenodo.org/record/11362245/files/figure.png" pageId="3" pageNumber="715" startId="3.[160,207,1614,1643]" targetBox="[151,1398,151,1987]" targetPageId="3" targetType="figure">
|
||
<paragraph id="8BF636AF682AFFC524DFF9F48B846A7B" blockId="3.[160,1392,1614,1987]" pageId="3" pageNumber="715">
|
||
<emphasis id="B93DEABD682AFFC524DFF9F48AA26B10" bold="true" box="[160,253,1614,1643]" pageId="3" pageNumber="715">Fig. 1.</emphasis>
|
||
Stages of development of an
|
||
<taxonomicName id="4C494D2C682AFFC526D2F9F489946B10" authorityName="Richard" authorityYear="1964" box="[685,971,1614,1643]" class="Trematoda" family="Echinostomatidae" genus="Echinostoma" kingdom="Animalia" order="Plagiorchiida" pageId="3" pageNumber="715" phylum="Platyhelminthes" rank="species" species="caproni">
|
||
<emphasis id="B93DEABD682AFFC526D2F9F489946B10" box="[685,971,1614,1643]" italics="true" pageId="3" pageNumber="715">Echinostoma caproni</emphasis>
|
||
</taxonomicName>
|
||
trematode (
|
||
<emphasis id="B93DEABD682AFFC52002F9F48EE36B11" box="[1149,1212,1614,1642]" italics="true" pageId="3" pageNumber="715">A–D</emphasis>
|
||
):
|
||
<emphasis id="B93DEABD682AFFC520A2F9F48EAC6B11" box="[1245,1267,1614,1642]" italics="true" pageId="3" pageNumber="715">A</emphasis>
|
||
— SEM microphotograph of a mother redia;
|
||
<emphasis id="B93DEABD682AFFC52605F9CE88D26BEB" box="[634,653,1652,1680]" italics="true" pageId="3" pageNumber="715">B</emphasis>
|
||
— histological section of a cercaria embryo in a daughter redia;
|
||
<emphasis id="B93DEABD682AFFC5248AF9238B556BCE" box="[245,266,1689,1717]" italics="true" pageId="3" pageNumber="715">C</emphasis>
|
||
— SEM micrograph of a cercaria with a discarded tail;
|
||
<emphasis id="B93DEABD682AFFC5207DF9208E466BCD" box="[1026,1049,1690,1718]" italics="true" pageId="3" pageNumber="715">D</emphasis>
|
||
— SEM micrograph of a metacercaria. Legend:
|
||
<emphasis id="B93DEABD682AFFC525AEF9048BAD6BA0" box="[465,498,1726,1755]" italics="true" pageId="3" pageNumber="715">bp</emphasis>
|
||
— birth pore,
|
||
<emphasis id="B93DEABD682AFFC526C4F90488936BA0" box="[699,716,1726,1755]" italics="true" pageId="3" pageNumber="715">h</emphasis>
|
||
— hemocytes,
|
||
<emphasis id="B93DEABD682AFFC527E2F90589E86BA0" box="[925,951,1727,1755]" italics="true" pageId="3" pageNumber="715">m</emphasis>
|
||
— mouth,
|
||
<emphasis id="B93DEABD682AFFC5202EF9058E336BA0" box="[1105,1132,1727,1755]" italics="true" pageId="3" pageNumber="715">os</emphasis>
|
||
— oral sucker,
|
||
<emphasis id="B93DEABD682AFFC52141F9058F176BA0" box="[1342,1352,1727,1755]" italics="true" pageId="3" pageNumber="715">t</emphasis>
|
||
— tail,
|
||
<emphasis id="B93DEABD682AFFC524A6F95E8AAC6A7B" box="[217,243,1764,1792]" italics="true" pageId="3" pageNumber="715">vs</emphasis>
|
||
— ventral sucker
|
||
</paragraph>
|
||
<paragraph id="8BF636AF682AFFC524DFF8AB8E3B6AB9" blockId="3.[160,1392,1614,1987]" pageId="3" pageNumber="715">
|
||
<emphasis id="B93DEABD682AFFC524DFF8AB8AAA6A56" bold="true" box="[160,245,1808,1837]" pageId="3" pageNumber="715">Рис. 1.</emphasis>
|
||
СтаΔии развития трематоΔ
|
||
<taxonomicName id="4C494D2C682AFFC5260BF8AA89DC6A56" authorityName="Richard" authorityYear="1964" box="[628,899,1808,1837]" class="Trematoda" family="Echinostomatidae" genus="Echinostoma" kingdom="Animalia" order="Plagiorchiida" pageId="3" pageNumber="715" phylum="Platyhelminthes" rank="species" species="caproni">
|
||
<emphasis id="B93DEABD682AFFC5260BF8AA89DC6A56" box="[628,899,1808,1837]" italics="true" pageId="3" pageNumber="715">Echinostoma caproni</emphasis>
|
||
</taxonomicName>
|
||
(
|
||
<emphasis id="B93DEABD682AFFC527EDF8AB898F6A56" box="[914,976,1809,1837]" italics="true" pageId="3" pageNumber="715">A–D</emphasis>
|
||
):
|
||
<emphasis id="B93DEABD682AFFC52799F8AB89A36A56" box="[998,1020,1809,1837]" italics="true" pageId="3" pageNumber="715">A</emphasis>
|
||
— SEM-микрофотография материнской реΔии;
|
||
<emphasis id="B93DEABD682AFFC525C6F88D8B936A28" box="[441,460,1847,1875]" italics="true" pageId="3" pageNumber="715">B</emphasis>
|
||
— гистоΛогический срез эмбриона церкарии в Δочерней реΔии;
|
||
<emphasis id="B93DEABD682AFFC52148F88C8F136A29" box="[1335,1356,1846,1874]" italics="true" pageId="3" pageNumber="715">C</emphasis>
|
||
— SEM-микрофотография церкарии с отброшенным хвостом;
|
||
<emphasis id="B93DEABD682AFFC5279FF8E689A86A03" box="[992,1015,1884,1912]" italics="true" pageId="3" pageNumber="715">D</emphasis>
|
||
— SEM-микрофотография метацеркарии. УсΛовные обозначения:
|
||
<emphasis id="B93DEABD682AFFC526C5F83A88846AE6" box="[698,731,1920,1949]" italics="true" pageId="3" pageNumber="715">bp</emphasis>
|
||
— роΔиΛьная пора;
|
||
<emphasis id="B93DEABD682AFFC52793F83A89A26AE6" box="[1004,1021,1920,1949]" italics="true" pageId="3" pageNumber="715">h</emphasis>
|
||
— гемоциты;
|
||
<emphasis id="B93DEABD682AFFC520C4F83B8E8A6AE6" box="[1211,1237,1921,1949]" italics="true" pageId="3" pageNumber="715">m</emphasis>
|
||
— ротовое отверстие;
|
||
<emphasis id="B93DEABD682AFFC52544F81D8B096AB8" box="[315,342,1959,1987]" italics="true" pageId="3" pageNumber="715">os</emphasis>
|
||
— ротовая присоска;
|
||
<emphasis id="B93DEABD682AFFC526F9F81D88CF6AB8" box="[646,656,1959,1987]" italics="true" pageId="3" pageNumber="715">t</emphasis>
|
||
— хвост;
|
||
<emphasis id="B93DEABD682AFFC52767F81D896D6AB8" box="[792,818,1959,1987]" italics="true" pageId="3" pageNumber="715">vs</emphasis>
|
||
— брюшная присоска
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BF636AF682DFFC224C2FF238BE96F8D" blockId="4.[151,756,153,1142]" pageId="4" pageNumber="716">
|
||
This artificial decrease of cercarial density led to the extension of the lifespan of infected snails (Group 2 — up to 56 days, Group 3 — up to 60 days). The experiment also confirmed the pathogenicity of metacercariae since snails from Group 4 died within 58 days. Therefore, the results of the experiments on Groups 2, 3, and 4 indicate that the mortality of snails is attributed to metacercarial infection. The analysis of the survivorship curves of different experimental groups confirms this assumption (
|
||
<figureCitation id="13722A2A682DFFC22506FD858B9A6F27" box="[377,453,575,604]" captionStart="Fig" captionStartId="4.[163,210,1814,1843]" captionTargetBox="[191,1359,1168,1798]" captionTargetId="figure-429@4.[191,1359,1168,1805]" captionTargetPageId="4" captionText="Fig. 2. Dynamics of mortality of Biomphalaria pfeifferi molluscs. Group 1* — red line / 38 days PI; Group 2* — green line / 56 days PI; Group 3* — blue line / 60 days PI; Group 4 — lilac line (magenta) / 58 days PI Рис. 2. Àинамика смертности моΛΛюсков Biomphalaria pfeifferi. Серия 1* — красная Λиния /38 Δней P. I.; Серия 2* — зеΛёная Λиния / 56 Δней P. I.; Серия 3* — гоΛубая Λиния / 60 Δней P. I.; Серия 4 — ΛиΛовая Λиния (magenta) / 58 Δней P. I." figureDoi="http://doi.org/10.5281/zenodo.11362249" httpUri="https://zenodo.org/record/11362249/files/figure.png" pageId="4" pageNumber="716">Fig. 2</figureCitation>
|
||
). The graph shows that snails from Group 1 started dying sooner and faster than snails from the other groups. At the same time, survivorship curves for Groups 2–4 look very similar.
|
||
</paragraph>
|
||
<paragraph id="8BF636AF682DFFC224C2FD458ED56DA7" blockId="4.[151,756,153,1142]" lastBlockId="4.[794,1399,153,412]" pageId="4" pageNumber="716">
|
||
These findings are also confirmed by statistical analysis. According to the Friedman test, the conditions of the experiment significantly affect the mortality dynamics of
|
||
<taxonomicName id="4C494D2C682DFFC224E8FC208B7B6ECE" baseAuthorityName="Krauss" baseAuthorityYear="1848" box="[151,292,921,950]" class="Gastropoda" family="Planorbidae" genus="Biomphalaria" kingdom="Animalia" order="Littorinimorpha" pageId="4" pageNumber="716" phylum="Mollusca" rank="species" species="pfeifferi">
|
||
<emphasis id="B93DEABD682DFFC224E8FC208B7B6ECE" box="[151,292,921,950]" italics="true" pageId="4" pageNumber="716">B. pfeifferi</emphasis>
|
||
</taxonomicName>
|
||
snails. A pairwise comparison of death times between Groups 1–4 (Wilcoxon test, including the Bonferroni correction) shows that the findings on Group 1 are different from the findings on Groups 2, 3, and 4 (p <0.001). At the same time, survivorship curves for Groups 2, 3 and 4 are statistically indistinguishable (p> 0.05).
|
||
</paragraph>
|
||
<paragraph id="8BF636AF682DFFC2273FFF5C8F286CE7" blockId="4.[794,1399,153,412]" pageId="4" pageNumber="716">
|
||
Thus, the analysis of survivorship curves of
|
||
<taxonomicName id="4C494D2C682DFFC22765FEB789C56C53" baseAuthorityName="Krauss" baseAuthorityYear="1848" box="[794,922,268,297]" class="Gastropoda" family="Planorbidae" genus="Biomphalaria" kingdom="Animalia" order="Littorinimorpha" pageId="4" pageNumber="716" phylum="Mollusca" rank="species" species="pfeifferi">
|
||
<emphasis id="B93DEABD682DFFC22765FEB789C56C53" box="[794,922,268,297]" italics="true" pageId="4" pageNumber="716">B. pfeifferi</emphasis>
|
||
</taxonomicName>
|
||
snails from different groups confirmed our assumption that
|
||
<taxonomicName id="4C494D2C682DFFC220E8FE898F416C34" authorityName="Richard" authorityYear="1964" box="[1175,1310,307,335]" class="Trematoda" family="Echinostomatidae" genus="Echinostoma" kingdom="Animalia" order="Plagiorchiida" pageId="4" pageNumber="716" phylum="Platyhelminthes" rank="species" species="caproni">
|
||
<emphasis id="B93DEABD682DFFC220E8FE898F416C34" box="[1175,1310,307,335]" italics="true" pageId="4" pageNumber="716">E. caproni</emphasis>
|
||
</taxonomicName>
|
||
metacercariae are pathogenic to snails regardless of whether they were initially exposed to rediae or not.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |