319 lines
56 KiB
XML
319 lines
56 KiB
XML
<document id="0C0E77C34E7894EB697DC5F52085A947" ID-CLB-Dataset="28050" ID-DOI="10.11646/zootaxa.4508.2.4" ID-GBIF-Dataset="d281727b-64ea-4ea1-a62e-86196701ad98" ID-ISSN="1175-5326" ID-Zenodo-Dep="2607016" ID-ZooBank="294C2462-ADF9-4C03-ABA0-81FC5B641EBA" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1553585805981" checkinUser="plazi" docAuthor="Pereira, Marcos W. O., Brito, Fabiano Carvalho, Eizirik, Eduardo & Utz, Laura R. P." docDate="2018" docId="497665455A65FF95FF36F8DAFCA143CE" docLanguage="en" docName="zootaxa.4508.2.4.pdf" docOrigin="Zootaxa 4508 (2)" docStyle="DocumentStyle:647186512141C8FC8976D5BCC54AEB7D.9:Zootaxa.2013-.journal_article" docStyleId="647186512141C8FC8976D5BCC54AEB7D" docStyleName="Zootaxa.2013-.journal_article" docStyleVersion="9" docTitle="Vorticella ampullaria Pereira & Brito & Eizirik & Utz 2018, n.sp." docType="treatment" docVersion="8" lastPageNumber="219" masterDocId="B54F1D3D5A61FF9DFFA1FF85FF80421E" masterDocTitle="Description of Two New Species of the Genus Vorticella (Ciliophora: Peritrichia) Epibionts on Pomacea canaliculata (Mollusca: Ampullariidae: Gastropoda) in Southern Brazil" masterLastPageNumber="224" masterPageNumber="211" pageNumber="215" updateTime="1698713012839" updateUser="ExternalLinkService">
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<mods:titleInfo id="66E8E85F095A67A120C34007ECC13B41">
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<mods:title id="2C1DDDBFC508606A4B6222808B0D7963">Description of Two New Species of the Genus Vorticella (Ciliophora: Peritrichia) Epibionts on Pomacea canaliculata (Mollusca: Ampullariidae: Gastropoda) in Southern Brazil</mods:title>
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<mods:name id="CD36CF2F9396B8890B85968B258BA060" type="personal">
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<mods:roleTerm id="75887817E30861F1C136FB4B09D0AABC">Author</mods:roleTerm>
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</mods:role>
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<mods:namePart id="5DAC29F1AE83C8DF546077D0E3D25337">Pereira, Marcos W. O.</mods:namePart>
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<mods:name id="E8FE52B5C24AE539953E86CE0B186762" type="personal">
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<mods:roleTerm id="E726F3C4C1FD04BD1D134F1EFF174705">Author</mods:roleTerm>
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</mods:role>
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<mods:namePart id="1CB54351BCC255198E8CC024021B88E1">Brito, Fabiano Carvalho</mods:namePart>
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</mods:name>
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<mods:name id="32C87E693E14E238BA8A00AF12900F72" type="personal">
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<mods:roleTerm id="6CA63661A448CABA9F62CA757A894EC3">Author</mods:roleTerm>
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</mods:role>
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<mods:namePart id="52A919BF77208A7E951A168D0B0E4111">Eizirik, Eduardo</mods:namePart>
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</mods:name>
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<mods:name id="61323C15688F1913FEF9AE1620DB495A" type="personal">
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<mods:roleTerm id="6E3AB38F54B0D7CA63418542C90A9BA4">Author</mods:roleTerm>
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</mods:role>
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<mods:namePart id="0F091D983876D11F3BC2E370DF733ACC">Utz, Laura R. P.</mods:namePart>
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<mods:typeOfResource id="E57FD75A1FEC0B9959DA4D2E6A7F0895">text</mods:typeOfResource>
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<mods:title id="20933291A55AE6715087DB8C7B8C7945">Zootaxa</mods:title>
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</mods:titleInfo>
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<mods:part id="C001AAD7026CAAD1A73ECDA234BF1764">
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<mods:date id="F1A99FC6F6782EBC386E263C3CE03268">2018</mods:date>
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<mods:number id="8B6ABB9BC75290FA0A82A7F84BF040A5">2018-10-31</mods:number>
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<mods:number id="E17710FD9C86FB2B7DC2C3FEA61A450B">4508</mods:number>
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<mods:detail id="524C9CF32CB8F801C116FFACB5A7018F" type="issue">
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<mods:identifier id="C27644B289C271A6C5AB96B468819937" type="DOI">10.11646/zootaxa.4508.2.4</mods:identifier>
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<mods:identifier id="0B9916A1FCFD32D4697D37918B8EDDD1" type="ISSN">1175-5326</mods:identifier>
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<treatment id="497665455A65FF95FF36F8DAFCA143CE" ID-DOI="http://doi.org/10.5281/zenodo.5962966" ID-GBIF-Taxon="154495762" ID-Zenodo-Dep="5962966" LSID="urn:lsid:plazi:treatment:497665455A65FF95FF36F8DAFCA143CE" httpUri="http://treatment.plazi.org/id/497665455A65FF95FF36F8DAFCA143CE" lastPageId="8" lastPageNumber="219" pageId="4" pageNumber="215">
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<subSubSection id="89C587D85A65FF99FF36F8DAFE594567" box="[151,473,1887,1913]" pageId="4" pageNumber="215" type="nomenclature">
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<paragraph id="C160D4535A65FF99FF36F8DAFE594567" blockId="4.[151,473,1887,1913]" box="[151,473,1887,1913]" pageId="4" pageNumber="215">
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<heading id="9A28633F5A65FF99FF36F8DAFE594567" bold="true" box="[151,473,1887,1913]" fontSize="11" level="1" pageId="4" pageNumber="215" reason="1">
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<emphasis id="F3AB08415A65FF99FF36F8DAFE594567" bold="true" box="[151,473,1887,1913]" pageId="4" pageNumber="215">
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<taxonomicName id="06DFAFD05A65FF99FF36F8DAFE184567" authority="Pereira & Brito & Eizirik & Utz, 2018" authorityName="Pereira & Brito & Eizirik & Utz" authorityYear="2018" box="[151,408,1887,1913]" class="Oligohymenophorea" family="Vorticellidae" genus="Vorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="4" pageNumber="215" phylum="Ciliophora" rank="species" species="ampullaria" status="sp. nov.">
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<emphasis id="F3AB08415A65FF99FF36F8DAFE184567" bold="true" box="[151,408,1887,1913]" italics="true" pageId="4" pageNumber="215">Vorticella ampullaria</emphasis>
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</taxonomicName>
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<taxonomicNameLabel id="E898B53A5A65FF99FE3FF8DAFE594567" box="[414,473,1887,1913]" pageId="4" pageNumber="215" rank="species">n.sp.</taxonomicNameLabel>
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</emphasis>
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</heading>
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</paragraph>
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</subSubSection>
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<subSubSection id="89C587D85A65FF98FF36F823FC7C4303" lastPageId="5" lastPageNumber="216" pageId="4" pageNumber="215" type="diagnosis">
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<paragraph id="C160D4535A65FF98FF36F823FC7C4303" blockId="4.[151,1436,1958,2019]" lastBlockId="5.[151,1437,151,501]" lastPageId="5" lastPageNumber="216" pageId="4" pageNumber="215">
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<emphasis id="F3AB08415A65FF99FF36F823FE9145A1" bold="true" box="[151,273,1958,1983]" pageId="4" pageNumber="215">Diagnosis.</emphasis>
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Freshwater peritrich with an elongate zooid measuring in vivo
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<date id="B561F2935A65FF99FC53F822FBD145A1" box="[1010,1105,1958,1983]" pageId="4" pageNumber="215" value="1970-10-25">70 X 25</date>
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µm on average. Presence of smooth silver line system on pellicle and narrow peristomial lip. A C-shaped macronucleus lies in the middle of the cell. Two contractile vacuoles are present: in oral and aboral regions. All infundibular polykinetids have three rows of kinetosomes each. The middle row of P1 is longer than others; the distal row of P1 is longer than row next to P2, which has different lengths adstomally and terminates at the adstomal curvature of P1. P3 terminates at the same level of P1 adstomally, with the middle row being longer at the abstomal end.
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</paragraph>
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</subSubSection>
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<subSubSection id="89C587D85A64FF98FF66FEA2FB65435E" box="[199,1253,295,320]" pageId="5" pageNumber="216" type="materials_examined">
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<paragraph id="C160D4535A64FF98FF66FEA2FB65435E" blockId="5.[151,1437,151,501]" box="[199,1253,295,320]" pageId="5" pageNumber="216">
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<materialsCitation id="71B7DE0E5A64FF98FF66FEA2FB65435E" ID-GBIF-Occurrence="2028138841" box="[199,1253,295,320]" country="Brazil" location="Rio Grande do Sul" longitude="-50.846386" pageId="5" pageNumber="216" specimenCount="1" stateProvince="Rio Grande do Sul" typeStatus="holotype">
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<emphasis id="F3AB08415A64FF98FF66FEA2FEE8435E" bold="true" box="[199,360,295,320]" pageId="5" pageNumber="216">
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<typeStatus id="1E646AF15A64FF98FF66FEA2FE82435E" box="[199,258,295,320]" pageId="5" pageNumber="216">Type</typeStatus>
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Locality
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</emphasis>
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. Patos Lagoon, Viamão (30°19’70”S;
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<geoCoordinate id="A4EBB2945A64FF98FCB7FEA2FC27435E" box="[790,935,295,320]" direction="west" orientation="longitude" pageId="5" pageNumber="216" precision="15" value="-50.846386">50°50’47”W</geoCoordinate>
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),
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<collectingRegion id="031B1AB15A64FF98FC1BFEA2FB0E435E" box="[954,1166,295,320]" country="Brazil" name="Rio Grande do Sul" pageId="5" pageNumber="216">Rio Grande do Sul</collectingRegion>
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,
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<collectingCountry id="B9C894C35A64FF98FB38FEA2FB61435E" box="[1177,1249,295,320]" name="Brazil" pageId="5" pageNumber="216">Brazil</collectingCountry>
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.
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</materialsCitation>
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</paragraph>
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</subSubSection>
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<subSubSection id="89C587D85A64FF98FF66FEC9FBB043EB" pageId="5" pageNumber="216" type="etymology">
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<paragraph id="C160D4535A64FF98FF66FEC9FB88437A" blockId="5.[151,1437,151,501]" box="[199,1032,332,357]" pageId="5" pageNumber="216">
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<emphasis id="F3AB08415A64FF98FF66FEC9FECE437B" bold="true" box="[199,334,332,357]" pageId="5" pageNumber="216">Etymology.</emphasis>
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The specific epithet refers to the family of the gastropod host.
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</paragraph>
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<paragraph id="C160D4535A64FF98FF66FEEAFBB043EB" blockId="5.[151,1437,151,501]" pageId="5" pageNumber="216">
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<emphasis id="F3AB08415A64FF98FF66FEEAFE274396" bold="true" box="[199,423,367,392]" pageId="5" pageNumber="216">Deposition of slide</emphasis>
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. Type material: A slide with a protargol-stained colony (
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<typeStatus id="1E646AF15A64FF98FB9BFEEAFB204396" box="[1082,1184,367,392]" pageId="5" pageNumber="216" type="holotype">holotype</typeStatus>
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||
) was deposited in the Protist Collection of the Museum of Science and Technology of the Pontifícia Universidade Católica do
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<collectingRegion id="031B1AB15A64FF98FAD5FE11FEB743CE" country="Brazil" name="Rio Grande do Sul" pageId="5" pageNumber="216">Rio Grande do Sul</collectingRegion>
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(PUCRS), under the number MCTP06. The
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<typeStatus id="1E646AF15A64FF98FC88FE3DFC0C43CE" box="[809,908,440,464]" pageId="5" pageNumber="216" type="paratype">paratype</typeStatus>
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, a slide with a marked protargol-stained colony was deposited in the Protist Collection of the Natural History Museum in London.
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</paragraph>
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</subSubSection>
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||
<caption id="95A084DB5A64FF98FF36F8C1FDAC45ED" httpUri="https://zenodo.org/record/2607020/files/figure.png" pageId="5" pageNumber="216" startId="5.[151,250,1860,1882]" targetBox="[151,1433,553,1833]" targetPageId="5">
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<paragraph id="C160D4535A64FF98FF36F8C1FDAC45ED" blockId="5.[151,1436,1860,2035]" pageId="5" pageNumber="216">
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<emphasis id="F3AB08415A64FF98FF36F8C1FE944547" bold="true" box="[151,276,1860,1882]" pageId="5" pageNumber="216">FIGURE 2.</emphasis>
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Schematic drawings of
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<taxonomicName id="06DFAFD05A64FF98FDAEF8C1FD774544" authority="Pereira & Brito & Eizirik & Utz, 2018" authorityName="Pereira & Brito & Eizirik & Utz" authorityYear="2018" box="[527,759,1860,1882]" class="Oligohymenophorea" family="Vorticellidae" genus="Uorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="5" pageNumber="216" phylum="Ciliophora" rank="species" species="veloxiiforme" status="sp. nov.">
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<emphasis id="F3AB08415A64FF98FDAEF8C1FD774544" box="[527,759,1860,1882]" italics="true" pageId="5" pageNumber="216">Uorticella veloxiiforme</emphasis>
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</taxonomicName>
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<emphasis id="F3AB08415A64FF98FD5EF8C1FCBA4547" bold="true" box="[767,826,1860,1881]" pageId="5" pageNumber="216">
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<taxonomicNameLabel id="E898B53A5A64FF98FD5EF8C1FCBA4547" box="[767,826,1860,1881]" pageId="5" pageNumber="216" rank="species">n. sp.</taxonomicNameLabel>
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</emphasis>
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and
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<taxonomicName id="06DFAFD05A64FF98FCCFF8C1FBC24544" authority="Pereira & Brito & Eizirik & Utz, 2018" authorityName="Pereira & Brito & Eizirik & Utz" authorityYear="2018" box="[878,1090,1860,1882]" class="Oligohymenophorea" family="Vorticellidae" genus="Uorticela" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="5" pageNumber="216" phylum="Ciliophora" rank="species" species="ampullaria" status="sp. nov.">
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<emphasis id="F3AB08415A64FF98FCCFF8C1FBC24544" box="[878,1090,1860,1882]" italics="true" pageId="5" pageNumber="216">Uorticela ampullaria</emphasis>
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</taxonomicName>
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<emphasis id="F3AB08415A64FF98FBEBF8C1FBFD4547" bold="true" box="[1098,1149,1860,1881]" pageId="5" pageNumber="216">
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<taxonomicNameLabel id="E898B53A5A64FF98FBEBF8C1FBFD4547" box="[1098,1149,1860,1881]" pageId="5" pageNumber="216" rank="species">sp.n.</taxonomicNameLabel>
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</emphasis>
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<emphasis id="F3AB08415A64FF98FB25F8C0FB4A4544" box="[1156,1226,1861,1882]" italics="true" pageId="5" pageNumber="216">in vivo</emphasis>
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and from protargolstained specimens:
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<emphasis id="F3AB08415A64FF98FEC5F8E7FE064566" bold="true" box="[356,390,1890,1912]" pageId="5" pageNumber="216">A.</emphasis>
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Live zooid of
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<taxonomicName id="06DFAFD05A64FF98FD8DF8E6FD474566" authorityName="Pereira & Brito & Eizirik & Utz" authorityYear="2018" box="[556,711,1890,1912]" class="Oligohymenophorea" family="Vorticellidae" genus="Uorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="5" pageNumber="216" phylum="Ciliophora" rank="species" species="veloxiiforme">
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<emphasis id="F3AB08415A64FF98FD8DF8E6FD474566" box="[556,711,1890,1912]" italics="true" pageId="5" pageNumber="216">U. veloxiiforme</emphasis>
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</taxonomicName>
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showing the contractile vacuole (CV) and a J-shaped macronucleus (MAC) (Bar 15 µm).
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<emphasis id="F3AB08415A64FF98FED4F804FE0C4588" bold="true" box="[373,396,1921,1942]" pageId="5" pageNumber="216">B.</emphasis>
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Protargol-stained zooid of
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<taxonomicName id="06DFAFD05A64FF98FD05F807FCBD4589" authorityName="Pereira & Brito & Eizirik & Utz" authorityYear="2018" box="[676,829,1921,1943]" class="Oligohymenophorea" family="Vorticellidae" genus="Uorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="5" pageNumber="216" phylum="Ciliophora" rank="species" species="veloxiiforme">
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<emphasis id="F3AB08415A64FF98FD05F807FCBD4589" box="[676,829,1921,1943]" italics="true" pageId="5" pageNumber="216">U. veloxiiforme</emphasis>
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</taxonomicName>
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showing the oral poliknetids inside the infundibular region (P1, P2, P3) (Bar 10 µm).
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<emphasis id="F3AB08415A64FF98FE09F81AFE4045AB" bold="true" box="[424,448,1951,1973]" pageId="5" pageNumber="216">C.</emphasis>
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Live zooid of
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<taxonomicName id="06DFAFD05A64FF98FDF8F824FD6445AB" authorityName="Pereira & Brito & Eizirik & Utz" authorityYear="2018" box="[601,740,1951,1974]" class="Oligohymenophorea" family="Vorticellidae" genus="Uorticela" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="5" pageNumber="216" phylum="Ciliophora" rank="species" species="ampullaria">
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<emphasis id="F3AB08415A64FF98FDF8F824FD6445AB" box="[601,740,1951,1974]" italics="true" pageId="5" pageNumber="216">U. ampullaria</emphasis>
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</taxonomicName>
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showing the position of the two contractile vacuoles (CV) and a Cshaped macronucleus (MAC) (Bar 15 µm).
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<emphasis id="F3AB08415A64FF98FDEEF83AFDE845CA" bold="true" box="[591,616,1983,2004]" pageId="5" pageNumber="216">D.</emphasis>
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Protargol-stained zooid of
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<taxonomicName id="06DFAFD05A64FF98FCDAF83AFB8345CA" authorityName="Pereira & Brito & Eizirik & Utz" authorityYear="2018" box="[891,1027,1982,2004]" class="Oligohymenophorea" family="Vorticellidae" genus="Uorticela" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="5" pageNumber="216" phylum="Ciliophora" rank="species" species="ampullaria">
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<emphasis id="F3AB08415A64FF98FCDAF83AFB8345CA" box="[891,1027,1982,2004]" italics="true" pageId="5" pageNumber="216">U. ampullaria</emphasis>
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</taxonomicName>
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showing the oral polykinetids inside the infundibulum (P1, P2, P3)
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<emphasis id="F3AB08415A64FF98FE09F859FE3045ED" bold="true" box="[424,432,2012,2035]" pageId="5" pageNumber="216">(</emphasis>
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Bar 10 µm).
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</paragraph>
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</caption>
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<subSubSection id="89C587D85A67FF95FF66FF12FCA143CE" lastPageId="8" lastPageNumber="219" pageId="6" pageNumber="217" type="materials_examined">
|
||
<paragraph id="C160D4535A67FF9BFF66FF12FE8643CE" blockId="6.[151,1436,151,464]" pageId="6" pageNumber="217">
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<emphasis id="F3AB08415A67FF9BFF66FF12FDBF42AE" bold="true" box="[199,575,151,176]" pageId="6" pageNumber="217">Morphology of live specimens.</emphasis>
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Elongate zooids, 69.8 µm long and 24.9 µm wide on average (
|
||
<tableCitation id="8C5DE1E85A67FF9BFA93FF12FA0A42AE" box="[1330,1418,151,176]" captionStart="TABLE 1" captionStartId="3.[151,239,620,643]" captionText="TABLE 1. Measurements of live specimens of V. veloxiiforme and V. ampullaria attached to Pomacea canaliculata from Patos Lagoon, Brazil. A total number of 25 zooids were measured for each character. All measurements are expressed in µm." pageId="6" pageNumber="217">Table 1</tableCitation>
|
||
), presenting a smooth striated pellicle (visible by high magnification and silver nitrate technique), narrow peristomial lip, and slightly elevated epistomial disk (
|
||
<figureCitation id="59E4C8D65A67FF9BFD53FF5AFCD242E6" box="[754,850,223,248]" captionStart="FIGURE 2" captionStartId="5.[151,250,1860,1882]" captionTargetBox="[151,1433,553,1833]" captionTargetId="figure@5.[151,1436,553,1838]" captionTargetPageId="5" captionText="FIGURE 2. Schematic drawings of Uorticella veloxiiforme n. sp. and Uorticela ampullaria sp.n. in vivo and from protargolstained specimens: A. Live zooid of U. veloxiiforme showing the contractile vacuole (CV) and a J-shaped macronucleus (MAC) (Bar 15 µm). B. Protargol-stained zooid of U. veloxiiforme showing the oral poliknetids inside the infundibular region (P1, P2, P3) (Bar 10 µm). C. Live zooid of U. ampullaria showing the position of the two contractile vacuoles (CV) and a Cshaped macronucleus (MAC) (Bar 15 µm). D. Protargol-stained zooid of U. ampullaria showing the oral polykinetids inside the infundibulum (P1, P2, P3) (Bar 10 µm)." httpUri="https://zenodo.org/record/2607020/files/figure.png" pageId="6" pageNumber="217">Figs. 2C</figureCitation>
|
||
and
|
||
<figureCitation id="59E4C8D65A67FF9BFC28FF65FC2F42E6" box="[905,943,224,248]" captionStart="FIGURE 4" captionStartId="7.[151,250,1483,1505]" captionTargetBox="[151,1436,215,1446]" captionTargetId="figure@7.[151,1436,215,1446]" captionTargetPageId="7" captionText="FIGURE 4. Uorticella ampullaria sp. n. live and protargol-stained specimens: A. Live zooid showing the position of the two contractile vacuoles (CV) and the spamoneme (SM) inside the basal stalk (Bar 10 µm). B. Live zooid showing the position of the aboral contractile vacuole (arrow) (Bar 10 µm). C. Live zooid showing the position of the oral contractile vacuole and Cshaped macronucleus (MAC) (Bar 10 µm). D. Protargol-stained zooid showing the oral polikinetids in the infundibular region (P1, P2, P3) (Bar 5 µm). E. Protargol-stained zooid showing details of the oral polikinetids 1 and 2 (Bar 5 µm). F. Protargolstained zooid showing the micro (MIC) and macronucleus (MAC) (Bar 5 µm)." httpUri="https://zenodo.org/record/2607024/files/figure.png" pageId="6" pageNumber="217">4A</figureCitation>
|
||
). The proportion between upper and middle of the body are similar. A peculiar characteristic for peritrich ciliates was observed: the presence of two contractile vacuoles (one close to the peristome and the other close to the scopula) (
|
||
<figureCitation id="59E4C8D65A67FF9BFC78FEA2FBBD435E" box="[985,1085,295,320]" captionStart="FIGURE 2" captionStartId="5.[151,250,1860,1882]" captionTargetBox="[151,1433,553,1833]" captionTargetId="figure@5.[151,1436,553,1838]" captionTargetPageId="5" captionText="FIGURE 2. Schematic drawings of Uorticella veloxiiforme n. sp. and Uorticela ampullaria sp.n. in vivo and from protargolstained specimens: A. Live zooid of U. veloxiiforme showing the contractile vacuole (CV) and a J-shaped macronucleus (MAC) (Bar 15 µm). B. Protargol-stained zooid of U. veloxiiforme showing the oral poliknetids inside the infundibular region (P1, P2, P3) (Bar 10 µm). C. Live zooid of U. ampullaria showing the position of the two contractile vacuoles (CV) and a Cshaped macronucleus (MAC) (Bar 15 µm). D. Protargol-stained zooid of U. ampullaria showing the oral polykinetids inside the infundibulum (P1, P2, P3) (Bar 10 µm)." httpUri="https://zenodo.org/record/2607020/files/figure.png" pageId="6" pageNumber="217">Figs. 2C</figureCitation>
|
||
,
|
||
<figureCitation id="59E4C8D65A67FF9BFBEBFEADFB5F435E" box="[1098,1247,295,320]" captionStart="FIGURE 4" captionStartId="7.[151,250,1483,1505]" captionTargetBox="[151,1436,215,1446]" captionTargetId="figure@7.[151,1436,215,1446]" captionTargetPageId="7" captionText="FIGURE 4. Uorticella ampullaria sp. n. live and protargol-stained specimens: A. Live zooid showing the position of the two contractile vacuoles (CV) and the spamoneme (SM) inside the basal stalk (Bar 10 µm). B. Live zooid showing the position of the aboral contractile vacuole (arrow) (Bar 10 µm). C. Live zooid showing the position of the oral contractile vacuole and Cshaped macronucleus (MAC) (Bar 10 µm). D. Protargol-stained zooid showing the oral polikinetids in the infundibular region (P1, P2, P3) (Bar 5 µm). E. Protargol-stained zooid showing details of the oral polikinetids 1 and 2 (Bar 5 µm). F. Protargolstained zooid showing the micro (MIC) and macronucleus (MAC) (Bar 5 µm)." httpUri="https://zenodo.org/record/2607024/files/figure.png" pageId="6" pageNumber="217">4A, B and C</figureCitation>
|
||
). The cytoplasm was colorless or slight greenish with many food vacuoles. A C-shape, elongate macronucleus was located vertically in the middle of the cell (
|
||
<figureCitation id="59E4C8D65A67FF9BFD83FEEAFD044396" box="[546,644,367,392]" captionStart="FIGURE 2" captionStartId="5.[151,250,1860,1882]" captionTargetBox="[151,1433,553,1833]" captionTargetId="figure@5.[151,1436,553,1838]" captionTargetPageId="5" captionText="FIGURE 2. Schematic drawings of Uorticella veloxiiforme n. sp. and Uorticela ampullaria sp.n. in vivo and from protargolstained specimens: A. Live zooid of U. veloxiiforme showing the contractile vacuole (CV) and a J-shaped macronucleus (MAC) (Bar 15 µm). B. Protargol-stained zooid of U. veloxiiforme showing the oral poliknetids inside the infundibular region (P1, P2, P3) (Bar 10 µm). C. Live zooid of U. ampullaria showing the position of the two contractile vacuoles (CV) and a Cshaped macronucleus (MAC) (Bar 15 µm). D. Protargol-stained zooid of U. ampullaria showing the oral polykinetids inside the infundibulum (P1, P2, P3) (Bar 10 µm)." httpUri="https://zenodo.org/record/2607020/files/figure.png" pageId="6" pageNumber="217">Figs. 2C</figureCitation>
|
||
, and
|
||
<figureCitation id="59E4C8D65A67FF9BFD61FEF5FD644396" box="[704,740,368,392]" captionStart="FIGURE 4" captionStartId="7.[151,250,1483,1505]" captionTargetBox="[151,1436,215,1446]" captionTargetId="figure@7.[151,1436,215,1446]" captionTargetPageId="7" captionText="FIGURE 4. Uorticella ampullaria sp. n. live and protargol-stained specimens: A. Live zooid showing the position of the two contractile vacuoles (CV) and the spamoneme (SM) inside the basal stalk (Bar 10 µm). B. Live zooid showing the position of the aboral contractile vacuole (arrow) (Bar 10 µm). C. Live zooid showing the position of the oral contractile vacuole and Cshaped macronucleus (MAC) (Bar 10 µm). D. Protargol-stained zooid showing the oral polikinetids in the infundibular region (P1, P2, P3) (Bar 5 µm). E. Protargol-stained zooid showing details of the oral polikinetids 1 and 2 (Bar 5 µm). F. Protargolstained zooid showing the micro (MIC) and macronucleus (MAC) (Bar 5 µm)." httpUri="https://zenodo.org/record/2607024/files/figure.png" pageId="6" pageNumber="217">4C</figureCitation>
|
||
). The scopula was rectangular-shaped, and the stalk measured 114.5 µm in length on average (
|
||
<tableCitation id="8C5DE1E85A67FF9BFE5CFE11FDD243B2" box="[509,594,404,429]" captionStart="TABLE 1" captionStartId="3.[151,239,620,643]" captionText="TABLE 1. Measurements of live specimens of V. veloxiiforme and V. ampullaria attached to Pomacea canaliculata from Patos Lagoon, Brazil. A total number of 25 zooids were measured for each character. All measurements are expressed in µm." pageId="6" pageNumber="217">Table 1</tableCitation>
|
||
). A spasmoneme with endoplasmic granules was observed inside the stalk (
|
||
<figureCitation id="59E4C8D65A67FF9BFF3EFE32FF7A43CE" box="[159,250,439,464]" captionStart="FIGURE 4" captionStartId="7.[151,250,1483,1505]" captionTargetBox="[151,1436,215,1446]" captionTargetId="figure@7.[151,1436,215,1446]" captionTargetPageId="7" captionText="FIGURE 4. Uorticella ampullaria sp. n. live and protargol-stained specimens: A. Live zooid showing the position of the two contractile vacuoles (CV) and the spamoneme (SM) inside the basal stalk (Bar 10 µm). B. Live zooid showing the position of the aboral contractile vacuole (arrow) (Bar 10 µm). C. Live zooid showing the position of the oral contractile vacuole and Cshaped macronucleus (MAC) (Bar 10 µm). D. Protargol-stained zooid showing the oral polikinetids in the infundibular region (P1, P2, P3) (Bar 5 µm). E. Protargol-stained zooid showing details of the oral polikinetids 1 and 2 (Bar 5 µm). F. Protargolstained zooid showing the micro (MIC) and macronucleus (MAC) (Bar 5 µm)." httpUri="https://zenodo.org/record/2607024/files/figure.png" pageId="6" pageNumber="217">Fig. 4A</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<caption id="95A084DB5A67FF9BFF36F818FAF745EE" httpUri="https://zenodo.org/record/2607022/files/figure.png" pageId="6" pageNumber="217" startId="6.[151,250,1949,1971]" targetBox="[151,1435,509,1859]" targetPageId="6">
|
||
<paragraph id="C160D4535A67FF9BFF36F818FAF745EE" blockId="6.[151,1436,1949,2032]" pageId="6" pageNumber="217">
|
||
<emphasis id="F3AB08415A67FF9BFF36F818FE9645AC" bold="true" box="[151,278,1949,1971]" pageId="6" pageNumber="217">FIGURE 3.</emphasis>
|
||
<taxonomicName id="06DFAFD05A67FF9BFEBEF818FD8B45AD" authority="Pereira & Brito & Eizirik & Utz, 2018" authorityName="Pereira & Brito & Eizirik & Utz" authorityYear="2018" box="[287,523,1949,1971]" class="Oligohymenophorea" family="Vorticellidae" genus="Uorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="6" pageNumber="217" phylum="Ciliophora" rank="species" species="veloxiiforme" status="sp. nov.">
|
||
<emphasis id="F3AB08415A67FF9BFEBEF818FD8B45AD" box="[287,523,1949,1971]" italics="true" pageId="6" pageNumber="217">Uorticella veloxiiforme</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="F3AB08415A67FF9BFDB5F818FDD045AC" bold="true" box="[532,592,1949,1970]" pageId="6" pageNumber="217">
|
||
<taxonomicNameLabel id="E898B53A5A67FF9BFDB5F818FDD045AC" box="[532,592,1949,1970]" pageId="6" pageNumber="217" rank="species">n. sp.</taxonomicNameLabel>
|
||
</emphasis>
|
||
silver nitrate-stained species and SEM photomicrographs:
|
||
<emphasis id="F3AB08415A67FF9BFB1CF818FB5745AC" bold="true" box="[1213,1239,1949,1970]" pageId="6" pageNumber="217">A.</emphasis>
|
||
Picture showing a zooid with horizontal striation and the trochal band (arrow) (Bar 10 µm).
|
||
<emphasis id="F3AB08415A67FF9BFC28F839FC2045CF" bold="true" box="[905,928,1980,2001]" pageId="6" pageNumber="217">B.</emphasis>
|
||
SEM micrograph of the zooid showing pellicular pores (arrow) (Bar 2 µm).
|
||
<emphasis id="F3AB08415A67FF9BFE05F85FFE3C45EE" bold="true" box="[420,444,2010,2032]" pageId="6" pageNumber="217">C.</emphasis>
|
||
Silver nitrate-stained zooid showing the silver lines on the pellicle (arrowheads) (Bar 20 µm).
|
||
</paragraph>
|
||
</caption>
|
||
<caption id="95A084DB5A66FF9AFF36FA4EFC344464" httpUri="https://zenodo.org/record/2607024/files/figure.png" pageId="7" pageNumber="218" startId="7.[151,250,1483,1505]" targetBox="[151,1436,215,1446]" targetPageId="7">
|
||
<paragraph id="C160D4535A66FF9AFF36FA4EFC344464" blockId="7.[151,1436,1483,1658]" pageId="7" pageNumber="218">
|
||
<emphasis id="F3AB08415A66FF9AFF36FA4EFE9347FE" bold="true" box="[151,275,1483,1505]" pageId="7" pageNumber="218">FIGURE 4.</emphasis>
|
||
<taxonomicName id="06DFAFD05A66FF9AFEBAFA4EFE7547FF" authority="Pereira & Brito & Eizirik & Utz, 2018" authorityName="Pereira & Brito & Eizirik & Utz" authorityYear="2018" box="[283,501,1483,1505]" class="Oligohymenophorea" family="Vorticellidae" genus="Uorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="7" pageNumber="218" phylum="Ciliophora" rank="species" species="ampullaria" status="sp. nov.">
|
||
<emphasis id="F3AB08415A66FF9AFEBAFA4EFE7547FF" box="[283,501,1483,1505]" italics="true" pageId="7" pageNumber="218">Uorticella ampullaria</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="F3AB08415A66FF9AFE5DFA4EFDB647FE" bold="true" box="[508,566,1483,1504]" pageId="7" pageNumber="218">
|
||
<taxonomicNameLabel id="E898B53A5A66FF9AFE5DFA4EFDB647FE" box="[508,566,1483,1504]" pageId="7" pageNumber="218" rank="species">sp. n.</taxonomicNameLabel>
|
||
</emphasis>
|
||
live and protargol-stained specimens:
|
||
<emphasis id="F3AB08415A66FF9AFC63FA4EFC5A47FE" bold="true" box="[962,986,1483,1504]" pageId="7" pageNumber="218">A.</emphasis>
|
||
Live zooid showing the position of the two contractile vacuoles (CV) and the spamoneme (SM) inside the basal stalk (Bar 10 µm).
|
||
<emphasis id="F3AB08415A66FF9AFBB8FA6FFBB047E1" bold="true" box="[1049,1072,1514,1535]" pageId="7" pageNumber="218">B.</emphasis>
|
||
Live zooid showing the position of the aboral contractile vacuole (arrow) (Bar 10 µm).
|
||
<emphasis id="F3AB08415A66FF9AFD0DF98DFD454400" bold="true" box="[684,709,1544,1566]" pageId="7" pageNumber="218">C.</emphasis>
|
||
Live zooid showing the position of the oral contractile vacuole and Cshaped macronucleus (MAC) (Bar 10 µm).
|
||
<emphasis id="F3AB08415A66FF9AFDF5F9A2FDED4422" bold="true" box="[596,621,1575,1596]" pageId="7" pageNumber="218">D.</emphasis>
|
||
Protargol-stained zooid showing the oral polikinetids in the infundibular region (P1, P2, P3) (Bar 5 µm).
|
||
<emphasis id="F3AB08415A66FF9AFE3DF9C3FE334445" bold="true" box="[412,435,1606,1627]" pageId="7" pageNumber="218">E.</emphasis>
|
||
Protargol-stained zooid showing details of the oral polikinetids 1 and 2 (Bar 5 µm).
|
||
<emphasis id="F3AB08415A66FF9AFABAF9C3FAAC4445" bold="true" box="[1307,1324,1606,1627]" pageId="7" pageNumber="218">F.</emphasis>
|
||
Protargolstained zooid showing the micro (MIC) and macronucleus (MAC) (Bar 5 µm).
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="C160D4535A66FF95FF66F923FA9242E6" blockId="7.[151,1436,1702,2015]" lastBlockId="8.[151,1437,151,464]" lastPageId="8" lastPageNumber="219" pageId="7" pageNumber="218">
|
||
<emphasis id="F3AB08415A66FF9AFF66F923FDE044A1" bold="true" box="[199,608,1702,1727]" pageId="7" pageNumber="218">Morphology of stained specimens.</emphasis>
|
||
The infraciliature and the nuclear apparatus of
|
||
<taxonomicName id="06DFAFD05A66FF9AFBDAF92DFA9544A1" authorityName="Pereira & Brito & Eizirik & Utz" authorityYear="2018" box="[1147,1301,1703,1727]" class="Oligohymenophorea" family="Vorticellidae" genus="Uorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="7" pageNumber="218" phylum="Ciliophora" rank="species" species="ampullaria">
|
||
<emphasis id="F3AB08415A66FF9AFBDAF92DFA9544A1" box="[1147,1301,1703,1727]" italics="true" pageId="7" pageNumber="218">V. ampullaria</emphasis>
|
||
</taxonomicName>
|
||
were easily revealed by silver staining (
|
||
<figureCitation id="59E4C8D65A66FF9AFE47F94FFDCE44FD" box="[486,590,1738,1763]" captionStart="FIGURE 2" captionStartId="5.[151,250,1860,1882]" captionTargetBox="[151,1433,553,1833]" captionTargetId="figure@5.[151,1436,553,1838]" captionTargetPageId="5" captionText="FIGURE 2. Schematic drawings of Uorticella veloxiiforme n. sp. and Uorticela ampullaria sp.n. in vivo and from protargolstained specimens: A. Live zooid of U. veloxiiforme showing the contractile vacuole (CV) and a J-shaped macronucleus (MAC) (Bar 15 µm). B. Protargol-stained zooid of U. veloxiiforme showing the oral poliknetids inside the infundibular region (P1, P2, P3) (Bar 10 µm). C. Live zooid of U. ampullaria showing the position of the two contractile vacuoles (CV) and a Cshaped macronucleus (MAC) (Bar 15 µm). D. Protargol-stained zooid of U. ampullaria showing the oral polykinetids inside the infundibulum (P1, P2, P3) (Bar 10 µm)." httpUri="https://zenodo.org/record/2607020/files/figure.png" pageId="7" pageNumber="218">Figs. 2D</figureCitation>
|
||
and
|
||
<figureCitation id="59E4C8D65A66FF9AFD31F94EFC8944FD" box="[656,777,1738,1763]" captionStart="FIGURE 4" captionStartId="7.[151,250,1483,1505]" captionTargetBox="[151,1436,215,1446]" captionTargetId="figure@7.[151,1436,215,1446]" captionTargetPageId="7" captionText="FIGURE 4. Uorticella ampullaria sp. n. live and protargol-stained specimens: A. Live zooid showing the position of the two contractile vacuoles (CV) and the spamoneme (SM) inside the basal stalk (Bar 10 µm). B. Live zooid showing the position of the aboral contractile vacuole (arrow) (Bar 10 µm). C. Live zooid showing the position of the oral contractile vacuole and Cshaped macronucleus (MAC) (Bar 10 µm). D. Protargol-stained zooid showing the oral polikinetids in the infundibular region (P1, P2, P3) (Bar 5 µm). E. Protargol-stained zooid showing details of the oral polikinetids 1 and 2 (Bar 5 µm). F. Protargolstained zooid showing the micro (MIC) and macronucleus (MAC) (Bar 5 µm)." httpUri="https://zenodo.org/record/2607024/files/figure.png" pageId="7" pageNumber="218">4D and E</figureCitation>
|
||
). All infundibular polykinetids presented three rows of kinetosomes (
|
||
<figureCitation id="59E4C8D65A66FF9AFE93F96BFE134519" box="[306,403,1774,1799]" captionStart="FIGURE 2" captionStartId="5.[151,250,1860,1882]" captionTargetBox="[151,1433,553,1833]" captionTargetId="figure@5.[151,1436,553,1838]" captionTargetPageId="5" captionText="FIGURE 2. Schematic drawings of Uorticella veloxiiforme n. sp. and Uorticela ampullaria sp.n. in vivo and from protargolstained specimens: A. Live zooid of U. veloxiiforme showing the contractile vacuole (CV) and a J-shaped macronucleus (MAC) (Bar 15 µm). B. Protargol-stained zooid of U. veloxiiforme showing the oral poliknetids inside the infundibular region (P1, P2, P3) (Bar 10 µm). C. Live zooid of U. ampullaria showing the position of the two contractile vacuoles (CV) and a Cshaped macronucleus (MAC) (Bar 15 µm). D. Protargol-stained zooid of U. ampullaria showing the oral polykinetids inside the infundibulum (P1, P2, P3) (Bar 10 µm)." httpUri="https://zenodo.org/record/2607020/files/figure.png" pageId="7" pageNumber="218">Figs. 2D</figureCitation>
|
||
and
|
||
<figureCitation id="59E4C8D65A66FF9AFE69F96AFDB44519" box="[456,564,1774,1799]" captionStart="FIGURE 4" captionStartId="7.[151,250,1483,1505]" captionTargetBox="[151,1436,215,1446]" captionTargetId="figure@7.[151,1436,215,1446]" captionTargetPageId="7" captionText="FIGURE 4. Uorticella ampullaria sp. n. live and protargol-stained specimens: A. Live zooid showing the position of the two contractile vacuoles (CV) and the spamoneme (SM) inside the basal stalk (Bar 10 µm). B. Live zooid showing the position of the aboral contractile vacuole (arrow) (Bar 10 µm). C. Live zooid showing the position of the oral contractile vacuole and Cshaped macronucleus (MAC) (Bar 10 µm). D. Protargol-stained zooid showing the oral polikinetids in the infundibular region (P1, P2, P3) (Bar 5 µm). E. Protargol-stained zooid showing details of the oral polikinetids 1 and 2 (Bar 5 µm). F. Protargolstained zooid showing the micro (MIC) and macronucleus (MAC) (Bar 5 µm)." httpUri="https://zenodo.org/record/2607024/files/figure.png" pageId="7" pageNumber="218">4D and E</figureCitation>
|
||
). P1 had three rows of different lengths in the adstomal end (the middle row is longer than the others; the distal row is longer than row next to P2) (
|
||
<figureCitation id="59E4C8D65A66FF9AFC2FF897FC6F4535" box="[910,1007,1810,1835]" captionStart="FIGURE 2" captionStartId="5.[151,250,1860,1882]" captionTargetBox="[151,1433,553,1833]" captionTargetId="figure@5.[151,1436,553,1838]" captionTargetPageId="5" captionText="FIGURE 2. Schematic drawings of Uorticella veloxiiforme n. sp. and Uorticela ampullaria sp.n. in vivo and from protargolstained specimens: A. Live zooid of U. veloxiiforme showing the contractile vacuole (CV) and a J-shaped macronucleus (MAC) (Bar 15 µm). B. Protargol-stained zooid of U. veloxiiforme showing the oral poliknetids inside the infundibular region (P1, P2, P3) (Bar 10 µm). C. Live zooid of U. ampullaria showing the position of the two contractile vacuoles (CV) and a Cshaped macronucleus (MAC) (Bar 15 µm). D. Protargol-stained zooid of U. ampullaria showing the oral polykinetids inside the infundibulum (P1, P2, P3) (Bar 10 µm)." httpUri="https://zenodo.org/record/2607020/files/figure.png" pageId="7" pageNumber="218">Figs. 2D</figureCitation>
|
||
and
|
||
<figureCitation id="59E4C8D65A66FF9AFB85F896FBC74535" box="[1060,1095,1811,1835]" captionStart="FIGURE 4" captionStartId="7.[151,250,1483,1505]" captionTargetBox="[151,1436,215,1446]" captionTargetId="figure@7.[151,1436,215,1446]" captionTargetPageId="7" captionText="FIGURE 4. Uorticella ampullaria sp. n. live and protargol-stained specimens: A. Live zooid showing the position of the two contractile vacuoles (CV) and the spamoneme (SM) inside the basal stalk (Bar 10 µm). B. Live zooid showing the position of the aboral contractile vacuole (arrow) (Bar 10 µm). C. Live zooid showing the position of the oral contractile vacuole and Cshaped macronucleus (MAC) (Bar 10 µm). D. Protargol-stained zooid showing the oral polikinetids in the infundibular region (P1, P2, P3) (Bar 5 µm). E. Protargol-stained zooid showing details of the oral polikinetids 1 and 2 (Bar 5 µm). F. Protargolstained zooid showing the micro (MIC) and macronucleus (MAC) (Bar 5 µm)." httpUri="https://zenodo.org/record/2607024/files/figure.png" pageId="7" pageNumber="218">4E</figureCitation>
|
||
); P2 also had rows of different lengths adstomally that terminated at the curvature of P1 (
|
||
<figureCitation id="59E4C8D65A66FF9AFC83F8B3FC044551" box="[802,900,1846,1871]" captionStart="FIGURE 2" captionStartId="5.[151,250,1860,1882]" captionTargetBox="[151,1433,553,1833]" captionTargetId="figure@5.[151,1436,553,1838]" captionTargetPageId="5" captionText="FIGURE 2. Schematic drawings of Uorticella veloxiiforme n. sp. and Uorticela ampullaria sp.n. in vivo and from protargolstained specimens: A. Live zooid of U. veloxiiforme showing the contractile vacuole (CV) and a J-shaped macronucleus (MAC) (Bar 15 µm). B. Protargol-stained zooid of U. veloxiiforme showing the oral poliknetids inside the infundibular region (P1, P2, P3) (Bar 10 µm). C. Live zooid of U. ampullaria showing the position of the two contractile vacuoles (CV) and a Cshaped macronucleus (MAC) (Bar 15 µm). D. Protargol-stained zooid of U. ampullaria showing the oral polykinetids inside the infundibulum (P1, P2, P3) (Bar 10 µm)." httpUri="https://zenodo.org/record/2607020/files/figure.png" pageId="7" pageNumber="218">Figs. 2D</figureCitation>
|
||
and
|
||
<figureCitation id="59E4C8D65A66FF9AFC1BF8B2FC5E4551" box="[954,990,1847,1871]" captionStart="FIGURE 4" captionStartId="7.[151,250,1483,1505]" captionTargetBox="[151,1436,215,1446]" captionTargetId="figure@7.[151,1436,215,1446]" captionTargetPageId="7" captionText="FIGURE 4. Uorticella ampullaria sp. n. live and protargol-stained specimens: A. Live zooid showing the position of the two contractile vacuoles (CV) and the spamoneme (SM) inside the basal stalk (Bar 10 µm). B. Live zooid showing the position of the aboral contractile vacuole (arrow) (Bar 10 µm). C. Live zooid showing the position of the oral contractile vacuole and Cshaped macronucleus (MAC) (Bar 10 µm). D. Protargol-stained zooid showing the oral polikinetids in the infundibular region (P1, P2, P3) (Bar 5 µm). E. Protargol-stained zooid showing details of the oral polikinetids 1 and 2 (Bar 5 µm). F. Protargolstained zooid showing the micro (MIC) and macronucleus (MAC) (Bar 5 µm)." httpUri="https://zenodo.org/record/2607024/files/figure.png" pageId="7" pageNumber="218">4E</figureCitation>
|
||
). P3 presented rows of same length that terminate at the level of P1 adstomally (
|
||
<figureCitation id="59E4C8D65A66FF9AFDC3F8DEFD46456D" box="[610,710,1883,1908]" captionStart="FIGURE 2" captionStartId="5.[151,250,1860,1882]" captionTargetBox="[151,1433,553,1833]" captionTargetId="figure@5.[151,1436,553,1838]" captionTargetPageId="5" captionText="FIGURE 2. Schematic drawings of Uorticella veloxiiforme n. sp. and Uorticela ampullaria sp.n. in vivo and from protargolstained specimens: A. Live zooid of U. veloxiiforme showing the contractile vacuole (CV) and a J-shaped macronucleus (MAC) (Bar 15 µm). B. Protargol-stained zooid of U. veloxiiforme showing the oral poliknetids inside the infundibular region (P1, P2, P3) (Bar 10 µm). C. Live zooid of U. ampullaria showing the position of the two contractile vacuoles (CV) and a Cshaped macronucleus (MAC) (Bar 15 µm). D. Protargol-stained zooid of U. ampullaria showing the oral polykinetids inside the infundibulum (P1, P2, P3) (Bar 10 µm)." httpUri="https://zenodo.org/record/2607020/files/figure.png" pageId="7" pageNumber="218">Figs. 2D</figureCitation>
|
||
and
|
||
<figureCitation id="59E4C8D65A66FF9AFCA1F8DEFCA1456D" box="[768,801,1883,1907]" captionStart="FIGURE 4" captionStartId="7.[151,250,1483,1505]" captionTargetBox="[151,1436,215,1446]" captionTargetId="figure@7.[151,1436,215,1446]" captionTargetPageId="7" captionText="FIGURE 4. Uorticella ampullaria sp. n. live and protargol-stained specimens: A. Live zooid showing the position of the two contractile vacuoles (CV) and the spamoneme (SM) inside the basal stalk (Bar 10 µm). B. Live zooid showing the position of the aboral contractile vacuole (arrow) (Bar 10 µm). C. Live zooid showing the position of the oral contractile vacuole and Cshaped macronucleus (MAC) (Bar 10 µm). D. Protargol-stained zooid showing the oral polikinetids in the infundibular region (P1, P2, P3) (Bar 5 µm). E. Protargol-stained zooid showing details of the oral polikinetids 1 and 2 (Bar 5 µm). F. Protargolstained zooid showing the micro (MIC) and macronucleus (MAC) (Bar 5 µm)." httpUri="https://zenodo.org/record/2607024/files/figure.png" pageId="7" pageNumber="218">4F</figureCitation>
|
||
). Abstomally, rows of P3 had different lengths with the middle row being longer than the other two (
|
||
<figureCitation id="59E4C8D65A66FF9AFD3BF8FBFD7D4589" box="[666,765,1918,1943]" captionStart="FIGURE 2" captionStartId="5.[151,250,1860,1882]" captionTargetBox="[151,1433,553,1833]" captionTargetId="figure@5.[151,1436,553,1838]" captionTargetPageId="5" captionText="FIGURE 2. Schematic drawings of Uorticella veloxiiforme n. sp. and Uorticela ampullaria sp.n. in vivo and from protargolstained specimens: A. Live zooid of U. veloxiiforme showing the contractile vacuole (CV) and a J-shaped macronucleus (MAC) (Bar 15 µm). B. Protargol-stained zooid of U. veloxiiforme showing the oral poliknetids inside the infundibular region (P1, P2, P3) (Bar 10 µm). C. Live zooid of U. ampullaria showing the position of the two contractile vacuoles (CV) and a Cshaped macronucleus (MAC) (Bar 15 µm). D. Protargol-stained zooid of U. ampullaria showing the oral polykinetids inside the infundibulum (P1, P2, P3) (Bar 10 µm)." httpUri="https://zenodo.org/record/2607020/files/figure.png" pageId="7" pageNumber="218">Figs. 2D</figureCitation>
|
||
and
|
||
<figureCitation id="59E4C8D65A66FF9AFC96F8FAFCD84589" box="[823,856,1919,1943]" captionStart="FIGURE 4" captionStartId="7.[151,250,1483,1505]" captionTargetBox="[151,1436,215,1446]" captionTargetId="figure@7.[151,1436,215,1446]" captionTargetPageId="7" captionText="FIGURE 4. Uorticella ampullaria sp. n. live and protargol-stained specimens: A. Live zooid showing the position of the two contractile vacuoles (CV) and the spamoneme (SM) inside the basal stalk (Bar 10 µm). B. Live zooid showing the position of the aboral contractile vacuole (arrow) (Bar 10 µm). C. Live zooid showing the position of the oral contractile vacuole and Cshaped macronucleus (MAC) (Bar 10 µm). D. Protargol-stained zooid showing the oral polikinetids in the infundibular region (P1, P2, P3) (Bar 5 µm). E. Protargol-stained zooid showing details of the oral polikinetids 1 and 2 (Bar 5 µm). F. Protargolstained zooid showing the micro (MIC) and macronucleus (MAC) (Bar 5 µm)." httpUri="https://zenodo.org/record/2607024/files/figure.png" pageId="7" pageNumber="218">4F</figureCitation>
|
||
). The micro and macronucleus are visible in silver stained specimens (
|
||
<figureCitation id="59E4C8D65A66FF9AFED2F826FE4A45A5" box="[371,458,1955,1980]" captionStart="FIGURE 4" captionStartId="7.[151,250,1483,1505]" captionTargetBox="[151,1436,215,1446]" captionTargetId="figure@7.[151,1436,215,1446]" captionTargetPageId="7" captionText="FIGURE 4. Uorticella ampullaria sp. n. live and protargol-stained specimens: A. Live zooid showing the position of the two contractile vacuoles (CV) and the spamoneme (SM) inside the basal stalk (Bar 10 µm). B. Live zooid showing the position of the aboral contractile vacuole (arrow) (Bar 10 µm). C. Live zooid showing the position of the oral contractile vacuole and Cshaped macronucleus (MAC) (Bar 10 µm). D. Protargol-stained zooid showing the oral polikinetids in the infundibular region (P1, P2, P3) (Bar 5 µm). E. Protargol-stained zooid showing details of the oral polikinetids 1 and 2 (Bar 5 µm). F. Protargolstained zooid showing the micro (MIC) and macronucleus (MAC) (Bar 5 µm)." httpUri="https://zenodo.org/record/2607024/files/figure.png" pageId="7" pageNumber="218">Fig. 4F</figureCitation>
|
||
). The silver nitrate technique, and scanning electron micrographs revealed a pattern of horizontal smooth silver lines in the pellicle (there were 54 between the peristomial lip and the trochal band, on average, and 14 between the trochal band and scopula, on average;
|
||
<tableCitation id="8C5DE1E85A69FF95FC3AFF12FC7042AE" box="[923,1008,151,176]" captionStart="TABLE 2" captionStartId="10.[151,239,152,175]" captionText="TABLE 2. Measurements of stained specimens of Vorticella veloxiiforme and Vorticella ampullaria attached to Pomacea canaliculata from Patos Lagoon, Brazil. Numbers in parenthesis refer to the total number of individuals measured for each character. All measurements are expressed in µm." pageId="8" pageNumber="219">Table 2</tableCitation>
|
||
), a diagnostic character of the genus
|
||
<taxonomicName id="06DFAFD05A69FF95FF36FF39FE8442CA" authorityName="Linnaeus" authorityYear="1767" box="[151,260,188,212]" class="Oligohymenophorea" family="Vorticellidae" genus="Vorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="8" pageNumber="219" phylum="Ciliophora" rank="genus">
|
||
<emphasis id="F3AB08415A69FF95FF36FF39FE8442CA" box="[151,260,188,212]" italics="true" pageId="8" pageNumber="219">Vorticella</emphasis>
|
||
</taxonomicName>
|
||
(
|
||
<figureCitation id="59E4C8D65A69FF95FEB2FF39FE6442CA" box="[275,484,188,213]" captionStart="FIGURE 5" captionStartId="8.[151,250,1895,1917]" captionTargetBox="[150,1436,532,1853]" captionTargetId="figure@8.[150,1436,532,1866]" captionTargetPageId="8" captionText="FIGURE 5. Uorticella ampullaria n. sp. silver nitrate-stained specimens and SEM photomicrographs: A. Picture showing details of the pores on the pellicle (arrow) (Bar 2 µm). B. SEM micrograph of the zooid showing the trochal band (arrow) (Bar 2.5 µm). C. Silver nitrate-stained zooid showing the silver lines on the pellicle (arrow) (Bar 4 µm). D. Silver nitrate-stained zooid showing the macronucleus (MAC), scopula(SC), and somatic myonemes (arrowhead)." httpUri="https://zenodo.org/record/2607026/files/figure.png" pageId="8" pageNumber="219">Figs. 5B, C and D</figureCitation>
|
||
). The presence of pores on the membrane was also observed with SEM. These pores were regularly organized on the pellicle as well as a horizontal pattern of silver lines (
|
||
<figureCitation id="59E4C8D65A69FF95FBF6FF5AFA8442E6" box="[1111,1284,223,248]" captionStart="FIGURE 4" captionStartId="7.[151,250,1483,1505]" captionTargetBox="[151,1436,215,1446]" captionTargetId="figure@7.[151,1436,215,1446]" captionTargetPageId="7" captionText="FIGURE 4. Uorticella ampullaria sp. n. live and protargol-stained specimens: A. Live zooid showing the position of the two contractile vacuoles (CV) and the spamoneme (SM) inside the basal stalk (Bar 10 µm). B. Live zooid showing the position of the aboral contractile vacuole (arrow) (Bar 10 µm). C. Live zooid showing the position of the oral contractile vacuole and Cshaped macronucleus (MAC) (Bar 10 µm). D. Protargol-stained zooid showing the oral polikinetids in the infundibular region (P1, P2, P3) (Bar 5 µm). E. Protargol-stained zooid showing details of the oral polikinetids 1 and 2 (Bar 5 µm). F. Protargolstained zooid showing the micro (MIC) and macronucleus (MAC) (Bar 5 µm)." httpUri="https://zenodo.org/record/2607024/files/figure.png" pageId="8" pageNumber="219">Figs. 4A and B</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="C160D4535A69FF95FF66FE81FCA143CE" blockId="8.[151,1437,151,464]" pageId="8" pageNumber="219">
|
||
<emphasis id="F3AB08415A69FF95FF66FE81FE334303" bold="true" box="[199,435,260,285]" pageId="8" pageNumber="219">Molecular analyses</emphasis>
|
||
<taxonomicName id="06DFAFD05A69FF95FE1EFE81FD454302" authority="Pereira & Brito & Eizirik & Utz, 2018" authorityName="Pereira & Brito & Eizirik & Utz" authorityYear="2018" box="[447,709,260,284]" class="Oligohymenophorea" family="Vorticellidae" genus="Vorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="8" pageNumber="219" phylum="Ciliophora" rank="species" species="veloxiiforme" status="sp. nov.">
|
||
<emphasis id="F3AB08415A69FF95FE1EFE81FD454302" box="[447,709,260,284]" italics="true" pageId="8" pageNumber="219">Vorticella veloxiiforme</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="E898B53A5A69FF95FD72FE80FCE04302" box="[723,864,260,285]" pageId="8" pageNumber="219" rank="variety">
|
||
n.sp. and
|
||
<taxonomicName id="06DFAFD05A69FF95FCEBFE80FCE04302" authority="Pereira & Brito & Eizirik & Utz, 2018" authorityName="Pereira & Brito & Eizirik & Utz" authorityYear="2018" box="[842,864,261,284]" class="Oligohymenophorea" family="Vorticellidae" genus="Uorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="8" pageNumber="219" phylum="Ciliophora" rank="species" species="ampullaria" status="sp. nov.">
|
||
<emphasis id="F3AB08415A69FF95FCEBFE80FCE04302" box="[842,864,261,284]" italics="true" pageId="8" pageNumber="219">V.</emphasis>
|
||
</taxonomicName>
|
||
</taxonomicNameLabel>
|
||
<emphasis id="F3AB08415A69FF95FCCDFE81FC684302" box="[876,1000,260,284]" italics="true" pageId="8" pageNumber="219">ampullaria</emphasis>
|
||
<taxonomicNameLabel id="E898B53A5A69FF95FC54FE80FBAA4302" box="[1013,1066,261,284]" pageId="8" pageNumber="219" rank="species">n.sp.</taxonomicNameLabel>
|
||
clustered with other
|
||
<taxonomicName id="06DFAFD05A69FF95FA8EFE81FA1C4302" authorityName="Linnaeus" authorityYear="1767" box="[1327,1436,260,284]" class="Oligohymenophorea" family="Vorticellidae" genus="Vorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="8" pageNumber="219" phylum="Ciliophora" rank="genus">
|
||
<emphasis id="F3AB08415A69FF95FA8EFE81FA1C4302" box="[1327,1436,260,284]" italics="true" pageId="8" pageNumber="219">Vorticella</emphasis>
|
||
</taxonomicName>
|
||
species within the peritrich order Vorticellida (
|
||
<bibRefCitation id="A54EA9A25A69FF95FD3FFEADFCC8435E" author="Utz, L. R. P. & Simao, T. L. L. & Safi, L. S. L. & Eizirik, E." box="[670,840,296,320]" pageId="8" pageNumber="219" pagination="415 - 420" refId="ref7022" refString="Utz, L. R. P., Simao, T. L. L., Safi, L. S. L. & Eizirik, E. (2010) Expanded Phylogenetic Representation of Genera Opercularia and Epistylis Sheds Light on the Evolution and Higher-Level Taxonomy of Peritrich Ciliates (Ciliophora: Peritrichia). Journal of Eukaryotic Microbiology, 57 (5), 415 - 420. https: // doi. org / 10.1111 / j. 1550 - 7408.2010.00497. x" type="journal article" year="2010">
|
||
Utz
|
||
<emphasis id="F3AB08415A69FF95FD6EFEACFC80435E" box="[719,768,296,320]" italics="true" pageId="8" pageNumber="219">et al</emphasis>
|
||
. 2010
|
||
</bibRefCitation>
|
||
) (
|
||
<figureCitation id="59E4C8D65A69FF95FCFFFEA2FC23435E" box="[862,931,295,320]" captionStart="FIGURE 6" captionStartId="9.[151,250,1875,1897]" captionTargetBox="[248,1337,213,1830]" captionTargetId="figure@9.[228,1357,193,1850]" captionTargetPageId="9" captionText="FIGURE 6. Maximum likelihood phylogeny (topology only) showing the placement of Uorticella veloxiiforme and Uorticella ampullaria (sequences in bold) in a phylogenetic tree of selected Peritrichia species, based on 18s rDNA sequences. Values above and below the branches indicate bootstrap values for adjacent nodes with Maximum likelihood (ML), Maximum parsimony (MP), and neighbor-joining (NJ), followed by the clade posterior probabilities (shown as percentages) estimated with BI. Bootstrap values are reported only for long branches or for the sequences obtained in this study." httpUri="https://zenodo.org/record/2607028/files/figure.png" pageId="8" pageNumber="219">Fig. 6</figureCitation>
|
||
).
|
||
<taxonomicName id="06DFAFD05A69FF95FC19FEACFBD0435E" authorityName="Pereira & Brito & Eizirik & Utz" authorityYear="2018" box="[952,1104,296,320]" class="Oligohymenophorea" family="Vorticellidae" genus="Uorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="8" pageNumber="219" phylum="Ciliophora" rank="species" species="ampullaria">
|
||
<emphasis id="F3AB08415A69FF95FC19FEACFBD0435E" box="[952,1104,296,320]" italics="true" pageId="8" pageNumber="219">V. ampullaria</emphasis>
|
||
</taxonomicName>
|
||
consistently clustered with
|
||
<taxonomicName id="06DFAFD05A69FF95FA26FEACFE98437A" class="Oligohymenophorea" family="Vorticellidae" genus="Vorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="8" pageNumber="219" phylum="Ciliophora" rank="species" species="convallaria">
|
||
<emphasis id="F3AB08415A69FF95FA26FEACFE98437A" italics="true" pageId="8" pageNumber="219">V. convallaria</emphasis>
|
||
</taxonomicName>
|
||
with all methods, a relationship that received moderate to high support (61–66% bootstrap support [BS] in ML, NJ and MP; 0.99 posterior probability [PP] in the BI). The position of
|
||
<taxonomicName id="06DFAFD05A69FF95FBE0FEF4FB684396" authorityName="Pereira & Brito & Eizirik & Utz" authorityYear="2018" box="[1089,1256,368,392]" class="Oligohymenophorea" family="Vorticellidae" genus="Vorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="8" pageNumber="219" phylum="Ciliophora" rank="species" species="veloxiiforme">
|
||
<emphasis id="F3AB08415A69FF95FBE0FEF4FB684396" box="[1089,1256,368,392]" italics="true" pageId="8" pageNumber="219">V. veloxiiforme</emphasis>
|
||
</taxonomicName>
|
||
was less stable, with most methods indicating a closer relationship with
|
||
<taxonomicName id="06DFAFD05A69FF95FCA7FE10FC0A43B2" box="[774,906,404,428]" class="Oligohymenophorea" family="Vorticellidae" genus="Vorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="8" pageNumber="219" phylum="Ciliophora" rank="species" species="aequilata">
|
||
<emphasis id="F3AB08415A69FF95FCA7FE10FC0A43B2" box="[774,906,404,428]" italics="true" pageId="8" pageNumber="219">V. aequilata</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="06DFAFD05A69FF95FC61FE10FBBD43B2" box="[960,1085,404,428]" class="Oligohymenophorea" family="Vorticellidae" genus="Vorticella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peritrichida" pageId="8" pageNumber="219" phylum="Ciliophora" rank="species" species="elongata">
|
||
<emphasis id="F3AB08415A69FF95FC61FE10FBBD43B2" box="[960,1085,404,428]" italics="true" pageId="8" pageNumber="219">V. elongata</emphasis>
|
||
</taxonomicName>
|
||
, albeit with limited support [18 and 43% BS in ML and MP, respectively; 0.74 PP in BI]).
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |