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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.77.59999" ID-GBIF-Dataset="d773c1ed-26b9-41e3-b1f2-2e0a49555a8a" ID-PMC="PMC7819952" ID-Pensoft-Pub="1314-4049-77-41" ID-Pensoft-UUID="FAE5387DCE535A42BEF6F4E81E9297AA" ID-PubMed="33519268" ModsDocID="1314-4049-77-41" checkinTime="1610662569213" checkinUser="pensoft" docAuthor="Yang, Qin, Jiang, Ning &amp; Tian, Cheng-Ming" docDate="2021" docId="FF202454C6E75DAFAFA6F783DE9AA27A" docLanguage="en" docName="MycoKeys 77: 41-64" docOrigin="MycoKeys 77" docPubDate="2021-01-14" docSource="http://dx.doi.org/10.3897/mycokeys.77.59999" docTitle="Diaporthe multiguttulata F. Huang, K. D. Hyde &amp; Hong Y. Li, in Huang et al., Fungal Biology 119 (5): 343. 2015." docType="treatment" docVersion="4" id="FAE5387DCE535A42BEF6F4E81E9297AA" lastPageNumber="41" masterDocId="FAE5387DCE535A42BEF6F4E81E9297AA" masterDocTitle="New species and records of Diaporthe from Jiangxi Province, China" masterLastPageNumber="64" masterPageNumber="41" pageNumber="41" updateTime="1668136606026" updateUser="ExternalLinkService">
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<mods:titleInfo>
<mods:title>New species and records of Diaporthe from Jiangxi Province, China</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Yang, Qin</mods:namePart>
<mods:affiliation>The Key Laboratory for Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing 100083, China &amp; Forestry Biotechnology Hunan Key Laboratories, Central South University of Forestry and Technology, Changsha 410004, China &amp; The Key Laboratory for Non-Wood Forest Cultivation and Conservation of the Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Jiang, Ning</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-9656-8500</mods:nameIdentifier>
<mods:affiliation>The Key Laboratory for Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing 100083, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Tian, Cheng-Ming</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-3352-7664</mods:nameIdentifier>
<mods:affiliation>The Key Laboratory for Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing 100083, China</mods:affiliation>
<mods:nameIdentifier type="email">chengmt@bjfu.edu.cn</mods:nameIdentifier>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
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<mods:title>MycoKeys</mods:title>
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<mods:part>
<mods:date>2021</mods:date>
<mods:detail type="pubDate">
<mods:number>2021-01-14</mods:number>
</mods:detail>
<mods:detail type="volume">
<mods:number>77</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>41</mods:start>
<mods:end>64</mods:end>
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<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.77.59999</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.77.59999</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-77-41</mods:identifier>
<mods:identifier type="Pensoft-UUID">FAE5387DCE535A42BEF6F4E81E9297AA</mods:identifier>
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<treatment ID-GBIF-Taxon="176584091" LSID="urn:lsid:plazi:treatment:FF202454C6E75DAFAFA6F783DE9AA27A" httpUri="http://treatment.plazi.org/id/FF202454C6E75DAFAFA6F783DE9AA27A" lastPageNumber="41" pageId="0" pageNumber="41">
<subSubSection pageId="0" pageNumber="41" type="nomenclature">
<paragraph pageId="0" pageNumber="41">
<taxonomicName LSID="FF202454-C6E7-5DAF-AFA6-F783DE9AA27A" authority="F. Huang, K. D. Hyde &amp; Hong Y. Li, in Huang et al., Fungal Biology 119 (5): 343. 2015." authorityName="F. Huang, K. D. Hyde &amp; Hong Y. Li, in Huang et al., Fungal Biology 119 (5): 343. 2015." class="Ascomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe multiguttulata" order="Diaporthales" pageId="0" pageNumber="41" phylum="Ascomycota" rank="species" species="multiguttulata">Diaporthe multiguttulata F. Huang, K.D. Hyde &amp; Hong Y. Li, in Huang et al., Fungal Biology 119(5): 343. 2015.</taxonomicName>
<figureCitation captionStart="Figure 6" captionStartId="F7" captionText="Figure 6. Diaporthe multiguttulata on Citrus maxima (BJFC-S 1614) a, b habit of conidiomata on twig c conidiomata on PDA d transverse section through conidiomata e longitudinal section through conidiomata f conidiogenous cells attached with alpha conidia g alpha conidia h the colony on PDA. Scale bars: 200 μm (b, d, e); 10 μm (f, g)." figureDoi="10.3897/mycokeys.77.59999.figure6" httpUri="https://binary.pensoft.net/fig/497466" pageId="0" pageNumber="41">Figure 6</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="41" type="description">
<paragraph pageId="0" pageNumber="41">Description.</paragraph>
<paragraph pageId="0" pageNumber="41">
Conidiomata pycnidial, 692-750(-800)
<normalizedToken originalValue="μm">μm</normalizedToken>
diam., solitary and with single necks erumpent through host bark. Tissue around neck is cylindrical. Locule circular, undivided, 450-565(-600)
<normalizedToken originalValue="μm">μm</normalizedToken>
diam. Conidiophores reduced to conidiogenous cells. Conidiogenous cells unbranched, straight or slightly curved, apical or base sometimes swelling, (8.5-)9-10.5(-11)
<normalizedToken originalValue="×">x</normalizedToken>
1.5-2
<normalizedToken originalValue="μm">μm</normalizedToken>
. Alpha conidia hyaline, aseptate, ellipsoidal, biguttulate or with one large guttulate, rounded at one end, slightly apex at the other end, occasionally submedian constriction, (7.5-)8-9(-10.5)
<normalizedToken originalValue="×">x</normalizedToken>
4-5(-5.5)
<normalizedToken originalValue="μm">μm</normalizedToken>
. Beta conidia not observed.
</paragraph>
<caption doi="10.3897/mycokeys.77.59999.figure6" httpUri="https://binary.pensoft.net/fig/497466" pageId="0" pageNumber="41" start="Figure 6" startId="F7">
<paragraph pageId="0" pageNumber="41">
<emphasis bold="true" pageId="0" pageNumber="41">Figure 6.</emphasis>
<taxonomicName class="Ascomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe multiguttulata" order="Diaporthales" pageId="0" pageNumber="41" phylum="Ascomycota" rank="species" species="multiguttulata">
<emphasis italics="true" pageId="0" pageNumber="41">Diaporthe multiguttulata</emphasis>
</taxonomicName>
on
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Citrus" higherTaxonomySource="GBIF,CoL" kingdom="Fungi" lsidName="Citrus maxima" order="Diaporthales" pageId="0" pageNumber="41" phylum="Ascomycota" rank="species" species="maxima">
<emphasis italics="true" pageId="0" pageNumber="41">Citrus maxima</emphasis>
</taxonomicName>
(BJFC-S1614)
<emphasis bold="true" pageId="0" pageNumber="41">a, b</emphasis>
habit of conidiomata on twig
<emphasis bold="true" pageId="0" pageNumber="41">c</emphasis>
conidiomata on PDA
<emphasis bold="true" pageId="0" pageNumber="41">d</emphasis>
transverse section through conidiomata
<emphasis bold="true" pageId="0" pageNumber="41">e</emphasis>
longitudinal section through conidiomata
<emphasis bold="true" pageId="0" pageNumber="41">f</emphasis>
conidiogenous cells attached with alpha conidia
<emphasis bold="true" pageId="0" pageNumber="41">g</emphasis>
alpha conidia
<emphasis bold="true" pageId="0" pageNumber="41">h</emphasis>
the colony on PDA. Scale bars: 200
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="41">b, d, e</emphasis>
); 10
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="41">f, g</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection pageId="0" pageNumber="41" type="description">
<paragraph pageId="0" pageNumber="41">Culture characters.</paragraph>
<paragraph pageId="0" pageNumber="41">Colony originally flat with white felty aerial mycelium, becoming pale green mycelium with age, margin area irregularly, with visible solitary conidiomata at maturity.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="41" type="materials_examined">
<paragraph pageId="0" pageNumber="41">Specimens examined.</paragraph>
<paragraph pageId="0" pageNumber="41">
China. Jiangxi Province: Ganzhou City, on branches of
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Citrus" higherTaxonomySource="GBIF,CoL" kingdom="Fungi" lsidName="Citrus maxima" order="Diaporthales" pageId="0" pageNumber="41" phylum="Ascomycota" rank="species" species="maxima">
<emphasis italics="true" pageId="0" pageNumber="41">Citrus maxima</emphasis>
</taxonomicName>
,
<geoCoordinate degrees="25" direction="north" minutes="51" orientation="latitude" precision="15" seconds="28" value="25.857779">25°51'28&quot;N</geoCoordinate>
,
<geoCoordinate degrees="114" direction="east" minutes="55" orientation="longitude" precision="15" seconds="19" value="114.92194">114°55'19&quot;E</geoCoordinate>
, 11 May 2018,
<emphasis italics="true" pageId="0" pageNumber="41">Q. Yang</emphasis>
,
<emphasis italics="true" pageId="0" pageNumber="41">Y. Liu</emphasis>
&amp;
<emphasis italics="true" pageId="0" pageNumber="41">Y.M. Liang</emphasis>
(BJFC-S1614; living culture: CFCC 53095, CFCC 53096 and CFCC 53097).
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="41" type="notes">
<paragraph pageId="0" pageNumber="41">Notes.</paragraph>
<paragraph pageId="0" pageNumber="41">
<taxonomicName class="Ascomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe multiguttulata" order="Diaporthales" pageId="0" pageNumber="41" phylum="Ascomycota" rank="species" species="multiguttulata">
<emphasis italics="true" pageId="0" pageNumber="41">Diaporthe multiguttulata</emphasis>
</taxonomicName>
was originally described as an endophyte from a healthy branch of
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Citrus" higherTaxonomySource="GBIF,CoL" kingdom="Fungi" lsidName="Citrus grandis" order="Diaporthales" pageId="0" pageNumber="41" phylum="Ascomycota" rank="species" species="grandis">
<emphasis italics="true" pageId="0" pageNumber="41">Citrus grandis</emphasis>
</taxonomicName>
in Fujian Province, China (
<bibRefCitation DOI="https://doi.org/10.1016/j.funbio.2015.02.006" author="Huang, F" journalOrPublisher="Fungal Biology" pageId="0" pageNumber="41" pagination="331 - 347" refId="B24" refString="Huang, F, Udayanga, D, Wang, X, Hou, X, Mei, X, Fu, Y, Hyde, KD, Li, HY, 2015. Endophytic Diaporthe associated with Citrus: A phylogenetic reassessment with seven new species from China. Fungal Biology 119: 331 - 347, DOI: https://doi.org/10.1016/j.funbio.2015.02.006" title="Endophytic Diaporthe associated with Citrus: A phylogenetic reassessment with seven new species from China." url="https://doi.org/10.1016/j.funbio.2015.02.006" volume="119" year="2015">Huang et al. 2015</bibRefCitation>
). In the present study, three isolates (CFCC 53095, CFCC 53096 and CFCC 53097) from symptomatic branches of
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Citrus" higherTaxonomySource="GBIF,CoL" kingdom="Fungi" lsidName="Citrus maxima" order="Diaporthales" pageId="0" pageNumber="41" phylum="Ascomycota" rank="species" species="maxima">
<emphasis italics="true" pageId="0" pageNumber="41">Citrus maxima</emphasis>
</taxonomicName>
were congruent with
<taxonomicName lsidName="D. multigutullata" pageId="0" pageNumber="41" rank="species" species="multigutullata">
<emphasis italics="true" pageId="0" pageNumber="41">D. multigutullata</emphasis>
</taxonomicName>
, based on DNA sequence data and confirmed from the morphological analysis (Fig.
<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Phylogram of Diaporthe from a Maximum Likelihood analysis based on combined ITS, cal, his 3, tef 1 and tub 2. Values above the branches indicate Maximum Likelihood bootstrap (left, ML BP ≥ 50 %) and Bayesian probabilities (right, BI PP ≥ 0.90). The tree is rooted with Diaporthella corylina. Strains in current study are in blue font and the ex-type cultures are in bold font." figureDoi="10.3897/mycokeys.77.59999.figure1" httpUri="https://binary.pensoft.net/fig/497460" pageId="0" pageNumber="41">1</figureCitation>
). The clade, therefore, was verified as
<taxonomicName lsidName="D. multigutullata" pageId="0" pageNumber="41" rank="species" species="multigutullata">
<emphasis italics="true" pageId="0" pageNumber="41">D. multigutullata</emphasis>
</taxonomicName>
which could exist both as an endophyte and a pathogen.
</paragraph>
</subSubSection>
</treatment>
</document>