New species and records of Diaporthe from Jiangxi Province, China Author Yang, Qin The Key Laboratory for Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing 100083, China & Forestry Biotechnology Hunan Key Laboratories, Central South University of Forestry and Technology, Changsha 410004, China & The Key Laboratory for Non-Wood Forest Cultivation and Conservation of the Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China Author Jiang, Ning https://orcid.org/0000-0002-9656-8500 The Key Laboratory for Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing 100083, China Author Tian, Cheng-Ming https://orcid.org/0000-0002-3352-7664 The Key Laboratory for Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing 100083, China chengmt@bjfu.edu.cn text MycoKeys 2021 2021-01-14 77 41 64 http://dx.doi.org/10.3897/mycokeys.77.59999 journal article http://dx.doi.org/10.3897/mycokeys.77.59999 1314-4049-77-41 FAE5387DCE535A42BEF6F4E81E9297AA Diaporthe multiguttulata F. Huang, K.D. Hyde & Hong Y. Li, in Huang et al., Fungal Biology 119(5): 343. 2015. Figure 6 Description. Conidiomata pycnidial, 692-750(-800) μm diam., solitary and with single necks erumpent through host bark. Tissue around neck is cylindrical. Locule circular, undivided, 450-565(-600) μm diam. Conidiophores reduced to conidiogenous cells. Conidiogenous cells unbranched, straight or slightly curved, apical or base sometimes swelling, (8.5-)9-10.5(-11) x 1.5-2 μm . Alpha conidia hyaline, aseptate, ellipsoidal, biguttulate or with one large guttulate, rounded at one end, slightly apex at the other end, occasionally submedian constriction, (7.5-)8-9(-10.5) x 4-5(-5.5) μm . Beta conidia not observed. Figure 6. Diaporthe multiguttulata on Citrus maxima (BJFC-S1614) a, b habit of conidiomata on twig c conidiomata on PDA d transverse section through conidiomata e longitudinal section through conidiomata f conidiogenous cells attached with alpha conidia g alpha conidia h the colony on PDA. Scale bars: 200 μm ( b, d, e ); 10 μm ( f, g ). Culture characters. Colony originally flat with white felty aerial mycelium, becoming pale green mycelium with age, margin area irregularly, with visible solitary conidiomata at maturity. Specimens examined. China. Jiangxi Province: Ganzhou City, on branches of Citrus maxima , 25°51'28"N , 114°55'19"E , 11 May 2018, Q. Yang , Y. Liu & Y.M. Liang (BJFC-S1614; living culture: CFCC 53095, CFCC 53096 and CFCC 53097). Notes. Diaporthe multiguttulata was originally described as an endophyte from a healthy branch of Citrus grandis in Fujian Province, China ( Huang et al. 2015 ). In the present study, three isolates (CFCC 53095, CFCC 53096 and CFCC 53097) from symptomatic branches of Citrus maxima were congruent with D. multigutullata , based on DNA sequence data and confirmed from the morphological analysis (Fig. 1 ). The clade, therefore, was verified as D. multigutullata which could exist both as an endophyte and a pathogen.