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<mods:title id="92EDFFDD81249F989DB87C26520B1F46">Orostylis gen. nov., a new genus of Dalytyphloplanida with seven new species (Platyhelminthes: Rhabdocoela)</mods:title>
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<taxonomicName id="BAC24D32FF9CF3735AD2F939FE90F896" authority="Van Steenkiste, Gobert &amp; Artois" box="[151,370,1783,1810]" family="Solenopharyngidae" genus="Orostylis" kingdom="Animalia" order="Rhabdocoela" pageId="6" pageNumber="35" phylum="Platyhelminthes" rank="species" species="donanae" status="sp. nov.">
<emphasis id="4FB6EAA3FF9CF3735AD2F939FE90F896" bold="true" box="[151,370,1783,1810]" italics="true" pageId="6" pageNumber="35">Orostylis donanae</emphasis>
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Van Steenkiste, Gobert &amp; Artois
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(
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,
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n. sp.
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Van Steenkiste
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. (2013)
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Neodalyellida sp.
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in
<bibRefCitation id="19534B40FF9CF3735B39F8B7FD91F815" author="Stephenson, I. &amp; Van Steenkiste, N. W. L. &amp; Leander, B. S." box="[380,627,1913,1937]" pageId="6" pageNumber="35" pagination="41 - 57" refId="ref10292" refString="Stephenson, I., Van Steenkiste, N. W. L. &amp; Leander, B. S. (2019) Molecular phylogeny of neodalyellid flatworms (Rhabdocoela), including three new species from British Columbia. Journal of Zoological Systematics and Evolutionary Research, 57, 41 - 57. https: // doi. org / 10.1111 / jzs. 12243" type="journal article" year="2019">
Stephenson
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(2019)
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.
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(
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locality.
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<geoCoordinate id="18F65076FF9DF3725BA8FF74FD98FF51" box="[493,634,186,213]" degrees="36" direction="north" minutes="47" orientation="latitude" pageId="7" pageNumber="36" precision="15" seconds="57" value="36.799168">36°4757”N</geoCoordinate>
;
<geoCoordinate id="18F65076FF9DF37258C3FF74FCEAFF51" box="[646,776,186,213]" degrees="6" direction="west" minutes="23" orientation="longitude" pageId="7" pageNumber="36" precision="15" seconds="21" value="-6.3891664">6°2321”W</geoCoordinate>
)
<location id="781D606AFF9DF372595DFF74FCB2FF50" LSID="urn:lsid:plazi:treatment:F56B87A7FF9CF37D5AD2F939FE49FE70:781D606AFF9DF372595DFF74FCB2FF50" box="[792,848,186,212]" country="Spain" county="Guadalquivir river" latitude="36.799168" longLatPrecision="19" longitude="-6.3891664" municipality="Donana" name="Sand" pageId="7" pageNumber="36" stateProvince="Andalucia">Sand</location>
from
<collectingMunicipality id="9D19ACCBFF9DF37259D0FF75FC0EFF51" box="[917,1004,187,213]" pageId="7" pageNumber="36">Doñana</collectingMunicipality>
beach sampled at the low water mark, at the
<collectingCounty id="941C4E3DFF9DF3725A99FF10FE49FF7C" box="[220,427,222,248]" pageId="7" pageNumber="36">Guadalquivir river</collectingCounty>
mouth (
<date id="097C1071FF9DF372584CFF10FD79FF7C" box="[521,667,222,248]" pageId="7" pageNumber="36" value="2008-04-06">
<collectingDate id="1938E999FF9DF372584CFF10FD79FF7C" box="[521,667,222,248]" pageId="7" pageNumber="36" value="2008-04-06">Apr. 6, 2008</collectingDate>
</date>
)
</materialsCitation>
.
</paragraph>
</subSubSection>
<caption id="29BD6639FF9DF3725AD2F947FF0AF889" ID-DOI="http://doi.org/10.5281/zenodo.6346921" ID-Zenodo-Dep="6346921" httpUri="https://zenodo.org/record/6346921/files/figure.png" pageId="7" pageNumber="36" startId="7.[151,250,1673,1697]" targetBox="[179,1407,272,1649]" targetPageId="7">
<paragraph id="7D7D36B1FF9DF3725AD2F947FF0AF889" blockId="7.[151,1436,1673,1805]" pageId="7" pageNumber="36">
<emphasis id="4FB6EAA3FF9DF3725AD2F947FEEFF925" bold="true" box="[151,269,1673,1697]" pageId="7" pageNumber="36">FIGURE 4</emphasis>
. Photos of live and whole-mounted specimens.
<emphasis id="4FB6EAA3FF9DF37258B0F947FCE5F925" bold="true" box="[757,775,1673,1697]" pageId="7" pageNumber="36">A</emphasis>
. Live specimen of
<taxonomicName id="BAC24D32FF9DF3725989F947FB4CF925" box="[972,1198,1673,1697]" family="Solenopharyngidae" genus="Orostylis" kingdom="Animalia" order="Rhabdocoela" pageId="7" pageNumber="36" phylum="Platyhelminthes" rank="species" species="asinaraensis" status="sp. nov.">
<emphasis id="4FB6EAA3FF9DF3725989F947FB4CF925" box="[972,1198,1673,1697]" italics="true" pageId="7" pageNumber="36">Orostylis asinaraensis</emphasis>
</taxonomicName>
<emphasis id="4FB6EAA3FF9DF3725EF0F947FAFCF925" bold="true" box="[1205,1310,1673,1697]" pageId="7" pageNumber="36">
<taxonomicNameLabel id="548557D8FF9DF3725EF0F947FAE4F925" box="[1205,1286,1673,1697]" pageId="7" pageNumber="36" rank="species">sp. nov.</taxonomicNameLabel>
B
</emphasis>
. Live specimen of
<taxonomicName id="BAC24D32FF9DF3725AA1F963FE69F941" box="[228,395,1709,1733]" family="Solenopharyngidae" genus="Orostylis" kingdom="Animalia" order="Rhabdocoela" pageId="7" pageNumber="36" phylum="Platyhelminthes" rank="species" species="caecus" status="sp. nov.">
<emphasis id="4FB6EAA3FF9DF3725AA1F963FE69F941" box="[228,395,1709,1733]" italics="true" pageId="7" pageNumber="36">Orostylis caecus</emphasis>
</taxonomicName>
<emphasis id="4FB6EAA3FF9DF3725BD4F963FE1BF941" bold="true" box="[401,505,1709,1733]" pageId="7" pageNumber="36">
<taxonomicNameLabel id="548557D8FF9DF3725BD4F963FE03F941" box="[401,481,1709,1733]" pageId="7" pageNumber="36" rank="species">sp. nov.</taxonomicNameLabel>
C
</emphasis>
. Live specimen of
<taxonomicName id="BAC24D32FF9DF37258FEF963FC94F941" box="[699,886,1709,1733]" family="Solenopharyngidae" genus="Orostylis" kingdom="Animalia" order="Rhabdocoela" pageId="7" pageNumber="36" phylum="Platyhelminthes" rank="species" species="distortus" status="sp. nov.">
<emphasis id="4FB6EAA3FF9DF37258FEF963FC94F941" box="[699,886,1709,1733]" italics="true" pageId="7" pageNumber="36">Orostylis distortus</emphasis>
</taxonomicName>
<emphasis id="4FB6EAA3FF9DF3725939F963FC06F941" bold="true" box="[892,996,1709,1733]" pageId="7" pageNumber="36">
<taxonomicNameLabel id="548557D8FF9DF3725939F963FC2EF941" box="[892,972,1709,1733]" pageId="7" pageNumber="36" rank="species">sp. nov.</taxonomicNameLabel>
D
</emphasis>
. Live specimen of
<taxonomicName id="BAC24D32FF9DF3725EE3F963FAA4F941" box="[1190,1350,1709,1733]" family="Solenopharyngidae" genus="Orostylis" kingdom="Animalia" order="Rhabdocoela" pageId="7" pageNumber="36" phylum="Platyhelminthes" rank="species" species="dohae" status="sp. nov.">
<emphasis id="4FB6EAA3FF9DF3725EE3F963FAA4F941" box="[1190,1350,1709,1733]" italics="true" pageId="7" pageNumber="36">Orostylis dohae</emphasis>
</taxonomicName>
<emphasis id="4FB6EAA3FF9DF3725F09F963FF4AF96D" bold="true" pageId="7" pageNumber="36">
<taxonomicNameLabel id="548557D8FF9DF3725F09F963FA7EF941" box="[1356,1436,1709,1733]" pageId="7" pageNumber="36" rank="species">sp. nov.</taxonomicNameLabel>
E
</emphasis>
. Stylet in whole-mounted specimen of
<taxonomicName id="BAC24D32FF9DF3725873F91FFD0DF96D" box="[566,751,1745,1769]" family="Solenopharyngidae" genus="Orostylis" kingdom="Animalia" order="Rhabdocoela" pageId="7" pageNumber="36" phylum="Platyhelminthes" rank="species" species="donanae" status="sp. nov.">
<emphasis id="4FB6EAA3FF9DF3725873F91FFD0DF96D" box="[566,751,1745,1769]" italics="true" pageId="7" pageNumber="36">Orostylis donanae</emphasis>
</taxonomicName>
<emphasis id="4FB6EAA3FF9DF37258B3F91FFCB9F96D" bold="true" box="[758,859,1745,1769]" pageId="7" pageNumber="36">
<taxonomicNameLabel id="548557D8FF9DF37258B3F91FFCA4F96D" box="[758,838,1745,1769]" pageId="7" pageNumber="36" rank="species">sp. nov.</taxonomicNameLabel>
F
</emphasis>
. Stylet in whole-mounted specimen of
<taxonomicName id="BAC24D32FF9DF3725EAFF91FFA7EF96D" box="[1258,1436,1745,1769]" family="Solenopharyngidae" genus="Orostylis" kingdom="Animalia" order="Rhabdocoela" pageId="7" pageNumber="36" phylum="Platyhelminthes" rank="species" species="gallicus" status="sp. nov.">
<emphasis id="4FB6EAA3FF9DF3725EAFF91FFA7EF96D" box="[1258,1436,1745,1769]" italics="true" pageId="7" pageNumber="36">Orostylis gallicus</emphasis>
</taxonomicName>
<taxonomicNameLabel id="548557D8FF9DF3725AD2F93BFF0AF889" box="[151,232,1781,1805]" pageId="7" pageNumber="36" rank="species">sp. nov.</taxonomicNameLabel>
</paragraph>
</caption>
<subSubSection id="35D8653AFF9DF3725A82F8F5FBE4F8FE" pageId="7" pageNumber="36" type="materials_examined">
<paragraph id="7D7D36B1FF9DF3725A82F8F5FBE4F8FE" blockId="7.[151,1436,1851,1950]" pageId="7" pageNumber="36">
<emphasis id="4FB6EAA3FF9DF3725A82F8F5FECDF8D1" bold="true" box="[199,303,1851,1877]" pageId="7" pageNumber="36">Material</emphasis>
.
<materialsCitation id="CDAA3CECFF9DF3725B04F8F5FC29F8FE" ID-GBIF-Occurrence="3699958304" collectionCode="FMNH, KV, XIV" pageId="7" pageNumber="36" specimenCount="1" typeStatus="holotype">
Observations on live specimens. Two whole mounts, one of which designated
<typeStatus id="A2798813FF9DF3725E90F8F5FAD5F8D1" box="[1237,1335,1851,1877]" pageId="7" pageNumber="36" type="holotype">holotype</typeStatus>
(
<collectionCode id="1BD3AE74FF9DF3725F0CF8F2FA7EF8D2" box="[1353,1436,1852,1878]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34795" lsid="urn:lsid:biocol.org:col:34795" name="Field Museum of Natural History" pageId="7" pageNumber="36" type="Museum">FMNH</collectionCode>
<collectionCode id="1BD3AE74FF9DF3725AD2F891FF22F8FD" box="[151,192,1887,1913]" pageId="7" pageNumber="36">KV</collectionCode>
.694), the other one deposited in the HU reference collection HU
<collectionCode id="1BD3AE74FF9DF37259DCF8AEFC29F8FE" box="[921,971,1888,1914]" pageId="7" pageNumber="36">XIV</collectionCode>
</materialsCitation>
.3.09.
</paragraph>
</subSubSection>
<subSubSection id="35D8653AFF9DF3725A82F84AFB5AF819" box="[199,1208,1923,1950]" pageId="7" pageNumber="36" type="etymology">
<paragraph id="7D7D36B1FF9DF3725A82F84AFB5AF819" blockId="7.[151,1436,1851,1950]" box="[199,1208,1923,1950]" pageId="7" pageNumber="36">
<emphasis id="4FB6EAA3FF9DF3725A82F84AFEAAF81A" bold="true" box="[199,328,1924,1950]" pageId="7" pageNumber="36">Etymology</emphasis>
. Species epithet named after the
<typeStatus id="A2798813FF9DF37258FEF84AFD09F81A" box="[699,747,1924,1950]" pageId="7" pageNumber="36">type</typeStatus>
locality in Doñana National Park,
<collectingCountry id="05D57621FF9DF3725E34F84DFB56F819" box="[1137,1204,1923,1949]" name="Spain" pageId="7" pageNumber="36">Spain</collectingCountry>
.
</paragraph>
</subSubSection>
<subSubSection id="35D8653AFF9DF37D5A82F805FE49FE70" lastPageId="8" lastPageNumber="37" pageId="7" pageNumber="36" type="description">
<paragraph id="7D7D36B1FF9DF37D5A82F805FE49FE70" blockId="7.[199,1437,1995,2022]" lastBlockId="8.[151,1437,150,501]" lastPageId="8" lastPageNumber="37" pageId="7" pageNumber="36">
<emphasis id="4FB6EAA3FF9DF3725A82F805FEB3F861" bold="true" box="[199,337,1995,2021]" pageId="7" pageNumber="36">Description</emphasis>
. Specimens have an elongate body with a pair of small, bean-shaped eyes near the anterior end. Whole-mounted specimens are about 0.5 (
<typeStatus id="A2798813FF92F37D5836FF58FD3AFF35" box="[627,728,150,177]" pageId="8" pageNumber="37" type="holotype">holotype</typeStatus>
) and 0.9 mm (HU XIV.3.09) long respectively. The pharynx is cylindrical in shape and located just behind the eyes. Based on observations of live specimens and whole mounts, the general internal organisation appears similar to that of
<taxonomicName id="BAC24D32FF92F37D5962FF10FC3AFF7C" box="[807,984,222,248]" family="Solenopharyngidae" genus="Orostylis" kingdom="Animalia" order="Rhabdocoela" pageId="8" pageNumber="37" phylum="Platyhelminthes" rank="species" species="dohae" status="sp. nov.">
<emphasis id="4FB6EAA3FF92F37D5962FF10FC3AFF7C" box="[807,984,222,248]" italics="true" pageId="8" pageNumber="37">Orostylis dohae</emphasis>
</taxonomicName>
<emphasis id="4FB6EAA3FF92F37D599AFF10FBDBFF7C" bold="true" box="[991,1081,222,248]" pageId="8" pageNumber="37">
<taxonomicNameLabel id="548557D8FF92F37D599AFF10FBDBFF7C" box="[991,1081,222,248]" pageId="8" pageNumber="37" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
, with two testes just behind the pharynx. The male copulatory organ is situated alongside the pharynx, in the anterior part of the body, with the distal end pointing anteriorly. The copulatory bulb contains an elongate seminal vesicle and connects distally to a sclerotised stylet. This stylet (
<emphasis id="4FB6EAA3FF92F37D5BE5FE84FDCFFEE0" bold="true" box="[416,557,330,356]" pageId="8" pageNumber="37">
<figureCitation id="E5F92A34FF92F37D5BE5FE84FDE3FEE0" box="[416,513,330,356]" captionStart="FIGURE 3" captionStartId="6.[151,250,1569,1593]" captionTargetBox="[159,1426,320,1538]" captionTargetId="figure-93@6.[151,1436,309,1546]" captionTargetPageId="6" captionText="FIGURE 3. Drawings of the stylets of the new species of Orostylis gen. nov. included in this work. A. Orostylis dohae sp. nov. BC. Orostylis asinaraensis sp. nov. D. Orostylis caecus sp. nov. E. Orostylis donanae sp. nov. F. Orostylis gallicus sp. nov. G. Orostylis distortus sp. nov. H. Orostylis timucuorum sp. nov. All drawings are from the holotypes, except for C and D which are from reference material. The stylets are oriented with the proximal base pointing to the top and the distal tip pointing to the bottom of the plate." figureDoi="http://doi.org/10.5281/zenodo.6346917" httpUri="https://zenodo.org/record/6346917/files/figure.png" pageId="8" pageNumber="37">Figs. 3E</figureCitation>
,
<figureCitation id="E5F92A34FF92F37D5849FE84FDCFFEE0" box="[524,557,330,356]" captionStart="FIGURE 4" captionStartId="7.[151,250,1673,1697]" captionTargetBox="[179,1407,272,1649]" captionTargetId="figure-66@7.[179,1407,272,1649]" captionTargetPageId="7" captionText="FIGURE 4. Photos of live and whole-mounted specimens. A. Live specimen of Orostylis asinaraensis sp. nov. B. Live specimen of Orostylis caecus sp. nov. C. Live specimen of Orostylis distortus sp. nov. D. Live specimen of Orostylis dohae sp. nov. E. Stylet in whole-mounted specimen of Orostylis donanae sp. nov. F. Stylet in whole-mounted specimen of Orostylis gallicus sp. nov." figureDoi="http://doi.org/10.5281/zenodo.6346921" httpUri="https://zenodo.org/record/6346921/files/figure.png" pageId="8" pageNumber="37">4E</figureCitation>
</emphasis>
) consists of a corkscrew-shaped folded plate or “gutter” and is 7494 µm long. The proximal base of the stylet is about 15 µm wide and bears an extension that is folded towards the anterior. As no serial slides were available for this species, it is not clear whether the male gonopore opens into the oral cavity, as is the case in
<taxonomicName id="BAC24D32FF92F37D5B0EFE78FE1EFE54" box="[331,508,438,464]" family="Solenopharyngidae" genus="Orostylis" kingdom="Animalia" order="Rhabdocoela" pageId="8" pageNumber="37" phylum="Platyhelminthes" rank="species" species="dohae" status="sp. nov.">
<emphasis id="4FB6EAA3FF92F37D5B0EFE78FE1EFE54" box="[331,508,438,464]" italics="true" pageId="8" pageNumber="37">Orostylis dohae</emphasis>
</taxonomicName>
<emphasis id="4FB6EAA3FF92F37D5846FE78FDBEFE54" bold="true" box="[515,604,438,464]" pageId="8" pageNumber="37">
<taxonomicNameLabel id="548557D8FF92F37D5846FE78FDBEFE54" box="[515,604,438,464]" pageId="8" pageNumber="37" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
No details on the anatomy of the female genital system could be observed on live specimens alone.
</paragraph>
</subSubSection>
</treatment>
</document>