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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.101.115370" ID-Pensoft-Pub="1314-4049-101-1" ID-Pensoft-UUID="FBF8EACC1A7B50CA918E4C7D2FB08E8E" ModsDocID="1314-4049-101-1" checkinTime="1704593452343" checkinUser="pensoft" docAuthor="He, Jiao, Li, De-Wei, Cui, Wen-Li &amp; Huang, Lin" docDate="2024" docId="6214D26A572553219BB3C5AA4938E345" docLanguage="en" docName="MycoKeys 101: 1-44" docOrigin="MycoKeys 101" docPubDate="2024-01-05" docSource="http://dx.doi.org/10.3897/mycokeys.101.115370" docTitle="Alternaria shandongensis Lin Huang, Jiao He &amp; D. W. Li 2024, sp. nov." docType="treatment" docVersion="2" id="FBF8EACC1A7B50CA918E4C7D2FB08E8E" lastPageNumber="1" masterDocId="FBF8EACC1A7B50CA918E4C7D2FB08E8E" masterDocTitle="Seven new species of Alternaria (Pleosporales, Pleosporaceae) associated with Chinese fir, based on morphological and molecular evidence" masterLastPageNumber="44" masterPageNumber="1" pageNumber="1" updateTime="1704593756116" updateUser="ExternalLinkService">
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<mods:title id="E1ACADF467FE4D40E2686A300534BA3F">Seven new species of Alternaria (Pleosporales, Pleosporaceae) associated with Chinese fir, based on morphological and molecular evidence</mods:title>
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<mods:namePart id="571506946F23C4AA0FF38E08BAD7AAC8">He, Jiao</mods:namePart>
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<mods:affiliation id="413FD670F1533296D8D1D8F5666DF7A1">Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China</mods:affiliation>
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<mods:namePart id="9F361E29E2768FD5CB3DE5740E93D105">Li, De-Wei</mods:namePart>
<mods:nameIdentifier id="0B551590A00A17517322AE4066EADE4E" type="ORCID">https://orcid.org/0000-0002-2788-7938</mods:nameIdentifier>
<mods:affiliation id="D2A5BA42F58CBBF6BE15AA50247B17BC">The Connecticut Agricultural Experiment Station Valley Laboratory, Windsor, CT 06095, USA</mods:affiliation>
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<mods:namePart id="9B18936DF02422A4EB658D002C8135FE">Cui, Wen-Li</mods:namePart>
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<mods:affiliation id="005223F0B21E5EBA97F78C6B1DDB358A">Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China</mods:affiliation>
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<mods:namePart id="B60006167CAF29DFFA09A4CF97331AF8">Huang, Lin</mods:namePart>
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<mods:affiliation id="7177D42C6386E7F98DEA248EE1D6031E">Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China</mods:affiliation>
<mods:nameIdentifier id="11DB465A72D33FC9C4436A5024B6853D" type="email">lhuang@njfu.edu.cn</mods:nameIdentifier>
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<mods:date id="14447D190946D2208CC73B667FCDE660">2024</mods:date>
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<mods:number id="F6FFADC09A1890AAB41B04D6A2A282C7">2024-01-05</mods:number>
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<mods:number id="5F9D2A91C43DD5D413CFC6D7CFB20BCB">101</mods:number>
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<treatment id="6214D26A572553219BB3C5AA4938E345" LSID="urn:lsid:plazi:treatment:6214D26A572553219BB3C5AA4938E345" httpUri="http://treatment.plazi.org/id/6214D26A572553219BB3C5AA4938E345" lastPageNumber="1" pageId="0" pageNumber="1">
<subSubSection id="09082AB22699ED2FAE78F1B18A958104" pageId="0" pageNumber="1" type="nomenclature">
<paragraph id="C8F4324F2FE71767E317BA262BD68F70" pageId="0" pageNumber="1">
<taxonomicName id="3D03F44A7476426D63201EA5769B93C5" LSID="6214D26A-5725-5321-9BB3-C5AA4938E345" authority="Lin Huang, Jiao He &amp; D. W. Li" authorityName="Lin Huang, Jiao He &amp; D. W. Li" authorityYear="2024" class="Dothideomycetes" family="Pleosporaceae" genus="Alternaria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Alternaria shandongensis" order="Pleosporales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="shandongensis" status="sp. nov.">Alternaria shandongensis Lin Huang, Jiao He &amp; D.W. Li</taxonomicName>
<taxonomicNameLabel id="7DC7C2442980150642C77120AD48B611" pageId="0" pageNumber="1">sp. nov.</taxonomicNameLabel>
</paragraph>
</subSubSection>
<subSubSection id="E6D2779A0F4C07DD4D97E3193C88BA3B" pageId="0" pageNumber="1" type="description">
<paragraph id="F462D4117D28BC2E32269170C52E280E" pageId="0" pageNumber="1">
<figureCitation id="D8E1ADA360924459BFB9001D68712056" captionStart="Figure 8" captionStartId="F8" captionText="Figure 8. Alternaria shandongensis (SDHG 12) A colony on PCA after 6 days at 25 ° C in the dark B-D sporulation patterns E, F conidiophores and conidiogenous cells G conidia. Scale bars: 50 μm (B, C); 10 μm (D-G)." figureDoi="10.3897/mycokeys.101.115370.figure8" httpUri="https://binary.pensoft.net/fig/962740" pageId="0" pageNumber="1">Fig. 8</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="D20DFD80B70E1EC551363188CF7D822A" pageId="0" pageNumber="1" type="holotype">
<paragraph id="E5B23D905A85BFCB6C922FC4A695942D" pageId="0" pageNumber="1">Holotype.</paragraph>
<paragraph id="D9B254EBDC30D115E10060F628F5205F" pageId="0" pageNumber="1">
China, Shandong Province, Yantai City, Penglai District, Hougou village,
<geoCoordinate id="A76F7DD9D489F2DB6FCE5EE17EB10490" degrees="37" direction="north" minutes="27" orientation="latitude" precision="15" seconds="32" value="37.45889">37°27'32&quot;N</geoCoordinate>
,
<geoCoordinate id="9BB75EBDB603EB4746A0D17EAAD63820" degrees="120" direction="east" minutes="46" orientation="longitude" precision="15" seconds="48" value="120.78001">120°46'48&quot;E</geoCoordinate>
, isolated from leaf spots of
<taxonomicName id="3C030E89BE500267AB1C0AB626549AEB" class="Mucoromycetes" family="Choanephoraceae" genus="Cunninghamia" kingdom="Fungi" lsidName="Cunninghamia lanceolata" order="Mucorales" pageId="0" pageNumber="1" phylum="Zygomycota" rank="species" species="lanceolata">
<emphasis id="DD5CBEA884B436BC7D8F17331F167FFA" italics="true" pageId="0" pageNumber="1">Cunninghamia lanceolata</emphasis>
</taxonomicName>
, May 2017, Wen-Li Cui, (holotype: CFCC 59354). Holotype specimen is a living specimen being maintained via lyophilisation at the China Forestry Culture Collection Center (CFCC). Ex-type (SDHG12) is maintained at the Forest Pathology Laboratory, Nanjing Forestry University.
</paragraph>
</subSubSection>
<subSubSection id="A40CB60584C104042FD575B0BA83FC62" pageId="0" pageNumber="1" type="etymology">
<paragraph id="8EA69C851752812DBD7DBDEB8C70ACCF" pageId="0" pageNumber="1">Etymology.</paragraph>
<paragraph id="4DB422F1FDB75428A22E96D8F26D39AC" pageId="0" pageNumber="1">Epithet is after Shandong Province where the type specimen was collected.</paragraph>
</subSubSection>
<subSubSection id="CD2B76765BC72F9C3AF9446692AC6743" pageId="0" pageNumber="1" type="distribution">
<paragraph id="00D3720471E4A33379A1B416DFE911AA" pageId="0" pageNumber="1">Host/distribution.</paragraph>
<paragraph id="2AAE534413DA357C3301009A0866E6AB" pageId="0" pageNumber="1">
From
<taxonomicName id="89625A8F02A0576DCCB73CC51C2B0623" authorityName="González-Sponga" authorityYear="1987" lsidName="C. lanceolata" pageId="0" pageNumber="1" rank="species" species="lanceolata">
<emphasis id="A700EF758DAB3B4BB16A44EA2C7D2CAB" italics="true" pageId="0" pageNumber="1">C. lanceolata</emphasis>
</taxonomicName>
in Hougou village, Penglai District, Yantai City, Shandong Province, China.
</paragraph>
</subSubSection>
<subSubSection id="E6D2ED0B1B6C1FAF9DD770613EAF12EE" pageId="0" pageNumber="1" type="description">
<paragraph id="0A397FB2FBBB7746B12010865E6EC3BB" pageId="0" pageNumber="1">Description.</paragraph>
<paragraph id="32BB7ABE04918F7A4133A72FDE78B995" pageId="0" pageNumber="1">
Mycelium
<normalizedToken id="38967B07492A624367BE22DD82B94A71" originalValue="superficial">superficial</normalizedToken>
on the PCA medium, composed of septate, branched, smooth, thin-walled, pale brown hyphae. Conidiophores solitary, emerging from aerial or creeping hyphae, straight or geniculate, simple or branched, with one or several apical conidiogenous loci, 1-5 septate, variable in length, (16.8-)23.6-51.1(-68.8)
<normalizedToken id="5F240B4DD68EE3DFF8BE72EB025A25EC" originalValue="×">x</normalizedToken>
(3.0-)3.4-4.3(-5.0)
<normalizedToken id="1C495779BD553845BA69D37F41FD76EF" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="A64B92DACD03D70105041AC8765B268A" originalValue="±">+/-</normalizedToken>
SD = 37.3
<normalizedToken id="6CF76A4445C8AAD88F870960480BB8E5" originalValue="±">+/-</normalizedToken>
13.8
<normalizedToken id="09E24397651497A789AF9E503E9B4A40" originalValue="×">x</normalizedToken>
3.8
<normalizedToken id="FD9CD3EDE9887C0FE2A5B9879FEF5999" originalValue="±">+/-</normalizedToken>
0.4
<normalizedToken id="9BBE935BD51B8275FC6BE74465D1C0FD" originalValue="μm">μm</normalizedToken>
, n = 35). Each conidiogenous locus bears a primary chain of 9-13 conidia; each primary chain usually has 1-3 lateral branches (secondary chains) of 1-2 conidia. Conidiogenous cells apical or subapical, cylindrical, light brown, smooth, (3.9-)4.8-9.6(-17.3)
<normalizedToken id="0627D64FFC7DD7DE2E69442A77DF72DB" originalValue="×">x</normalizedToken>
(2.5-)3.2-4.3(-4.8)
<normalizedToken id="1DE67DF8031A4AB1A3BEE6F72C5A72D3" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="DE86423E3B33E675543422182E7307E2" originalValue="±">+/-</normalizedToken>
SD = 7.2
<normalizedToken id="AED6913282025CF910FE3C9B4D1854D2" originalValue="±">+/-</normalizedToken>
2.4
<normalizedToken id="D054E10B97DCF095E09FF72D61685B65" originalValue="×">x</normalizedToken>
3.7
<normalizedToken id="3CD81B06A4B89E6D2F9C4F28704DED98" originalValue="±">+/-</normalizedToken>
0.6
<normalizedToken id="F02876CF919E7AD5ADDD76E566DD37E8" originalValue="μm">μm</normalizedToken>
, n = 46), mono- or polytretic. Conidial bodies ovoid to ellipsoid, brown to dark brown, (14.8-)20.1-31.2(-51.5)
<normalizedToken id="D18CB7409FDE8D520ADBE7D155835E96" originalValue="×">x</normalizedToken>
(7.5-)9.3-14.1(-17.0)
<normalizedToken id="2F923A942F19FCD62502C7FADA1A1F33" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="0691B970F3660821AAE79A107D6F8707" originalValue="±">+/-</normalizedToken>
SD = 25.6
<normalizedToken id="18ADE14BF53D19779F30541A24DA960C" originalValue="±">+/-</normalizedToken>
5.6
<normalizedToken id="344E302794DF4011AA5886495CE0159E" originalValue="×">x</normalizedToken>
11.7
<normalizedToken id="E4639899735D7CF3A7A630B62E170117" originalValue="±">+/-</normalizedToken>
2.4
<normalizedToken id="2BE17E93938E9C12650B43F961AE9DE7" originalValue="μm">μm</normalizedToken>
, n = 66), with 2-7 transverse and 0-3 longitudinal septa, mostly smooth to occasionally roughened. Secondary conidia commonly produced via a short lateral secondary conidiophore. Secondary conidiophores (false beaks) at the apical end and median of conidium, short, mostly single-celled, (2.9-)2.7-10.3(-23.5)
<normalizedToken id="DC4B8E793D83C5F61FB08DD7975FC7F6" originalValue="μm">μm</normalizedToken>
<normalizedToken id="8D3AC9662F4A913CE16D008152AC0657" originalValue="×">x</normalizedToken>
(2.0-)2.3-3.1(-3.7)
<normalizedToken id="3934E2B16E161EA98581D81931A4B393" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="0A4CB4179B1AB5B2D5231F82D3A0E41A" originalValue="±">+/-</normalizedToken>
SD = 6.5
<normalizedToken id="EF4A1F1907B06AAADEED7AA9929DBCA4" originalValue="±">+/-</normalizedToken>
3.9
<normalizedToken id="286A2B74E49492F86615E9CB1640998A" originalValue="μm">μm</normalizedToken>
<normalizedToken id="18E593BBD04B2F0409086E65F39BBF61" originalValue="×">x</normalizedToken>
2.7
<normalizedToken id="50CC3A88B90AFCC388DF17DE01324C64" originalValue="±">+/-</normalizedToken>
0.4
<normalizedToken id="F0FE33BF7EA699106DAB0306C2F2C970" originalValue="μm">μm</normalizedToken>
, n = 34). Conidial beakless mostly with a conical cell at the apex. Chlamydospores not observed.
</paragraph>
<caption id="07D49C70CC4B585C615293262B86A164" doi="10.3897/mycokeys.101.115370.figure8" httpUri="https://binary.pensoft.net/fig/962740" pageId="0" pageNumber="1" start="Figure 8" startId="F8">
<paragraph id="9F8733A36D62107C82DC4AF859C95FDA" pageId="0" pageNumber="1">
<emphasis id="9CD7F7DE708A1CA41C7520AC9E83F5D4" bold="true" pageId="0" pageNumber="1">Figure 8.</emphasis>
<taxonomicName id="F693A1ACFFE99D34282E44F7D1BF2BF4" authorityName="Lin Huang, Jiao He &amp; D. W. Li" authorityYear="2024" class="Dothideomycetes" family="Moraceae" genus="Alternaria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Alternaria shandongensis" order="Pleosporales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="shandongensis">
<emphasis id="6592ED07895438D8709D0EAFB606FD7B" italics="true" pageId="0" pageNumber="1">Alternaria shandongensis</emphasis>
</taxonomicName>
(SDHG12)
<emphasis id="5CEBFFC10E48CE7EBA1523A98F3B67D8" bold="true" pageId="0" pageNumber="1">A</emphasis>
colony on PCA after 6 days at 25 °C in the dark
<emphasis id="E2FD3482662773D40A60A5841803332A" bold="true" pageId="0" pageNumber="1">B-D</emphasis>
sporulation patterns
<emphasis id="F04918BFFF3D788C7F7F77BD89AB3815" bold="true" pageId="0" pageNumber="1">E, F</emphasis>
conidiophores and conidiogenous cells
<emphasis id="6B5F0AA2FEF9BEDAE2D600CB68AA4B76" bold="true" pageId="0" pageNumber="1">G</emphasis>
conidia. Scale bars: 50
<normalizedToken id="412D26D8016DF1341DAA4507560C08A9" originalValue="μm">μm</normalizedToken>
(
<emphasis id="F4D2FC28B5E4B5961B7469B09B0210E3" bold="true" pageId="0" pageNumber="1">B, C</emphasis>
); 10
<normalizedToken id="8E43D03652EE494F5FD78F6390DBF700" originalValue="μm">μm</normalizedToken>
(
<emphasis id="84781E6A76BFD9FF306F7F12F9CFD22C" bold="true" pageId="0" pageNumber="1">D-G</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection id="65D3A95AB1747AC25B6BBED376CC5C39" pageId="0" pageNumber="1" type="description">
<paragraph id="6C41BB5033295821C9F6A91A3A3911B4" pageId="0" pageNumber="1">Culture characteristics.</paragraph>
<paragraph id="059D9CFF850A90761E9E910F79DE2B4D" pageId="0" pageNumber="1">
Colonies on PCA incubated at 25 °C in the dark growing at 7.6
<normalizedToken id="2D9850F08E03478A2C61980A9F5EA538" originalValue="±">+/-</normalizedToken>
0.7 mm/d; aerial hypha sparse, dark green to black; reverse centre grey, sporulation abundant; diffusible pigment absent.
</paragraph>
</subSubSection>
<subSubSection id="286CEFB1439F43623CD5B6B46E178CB4" pageId="0" pageNumber="1" type="materials_examined">
<paragraph id="F8BE6CD9F0B779776D1968B1B61EAC79" pageId="0" pageNumber="1">Additional materials examined.</paragraph>
<paragraph id="655BE769324E291F70F049EDB0FACC6F" pageId="0" pageNumber="1">
<materialsCitation id="F2DA7C00D290CC4E068B7ECDB4D060EA" collectingDate="2017-05" country="China" county="Yantai City" latitude="37.45889" location="Hougou village" longLatPrecision="19" longitude="120.78001" municipality="Penglai District" specimenCount="1" stateProvince="Shandong Province">
<collectingCountry id="7C39A608562E5278B4960A1377732469" name="China">China</collectingCountry>
,
<collectingRegion id="ED0E8236F58A11B585FBA256BC0A66F2" country="China" name="Shandong">Shandong Province</collectingRegion>
,
<collectingCounty id="84D0D27EE72FBA369134C5D41943B2A0">Yantai City</collectingCounty>
,
<collectingMunicipality id="96E400D8717533DBCE44FB37C2C92A5B">Penglai District</collectingMunicipality>
,
<location id="D6EE8B63D5D957CC1AF91C08DAC1DB7E" LSID="urn:lsid:plazi:treatment:6214D26A572553219BB3C5AA4938E345:D6EE8B63D5D957CC1AF91C08DAC1DB7E" country="China" county="Yantai City" latitude="37.45889" longLatPrecision="19" longitude="120.78001" municipality="Penglai District" name="Hougou village" stateProvince="Shandong Province">Hougou village</location>
,
<geoCoordinate id="2B62B19D9856241C31D467C5A6FD863C" degrees="37" direction="north" minutes="27" orientation="latitude" precision="15" seconds="32" value="37.45889">37°27'32&quot;N</geoCoordinate>
,
<geoCoordinate id="BF84B52DF4CBE52DF2D7D4B3110D931E" degrees="120" direction="east" minutes="46" orientation="longitude" precision="15" seconds="48" value="120.78001">120°46'48&quot;E</geoCoordinate>
, isolated from leaf spots of
<taxonomicName id="FC83934FE4C490D550B2A391409C740A" class="Mucoromycetes" family="Choanephoraceae" genus="Cunninghamia" kingdom="Fungi" lsidName="Cunninghamia lanceolata" order="Mucorales" pageId="0" pageNumber="1" phylum="Zygomycota" rank="species" species="lanceolata">
<emphasis id="313359BD880C5B6046EF9EDB92BEC7D4" italics="true" pageId="0" pageNumber="1">Cunninghamia lanceolata</emphasis>
</taxonomicName>
,
<collectingDate id="85586EF71402018FA39DD668CD89E2C4" value="2017-05">May 2017</collectingDate>
, Wen-Li Cui, SDHG12-1, SDHG12-2, SDHG12-3, SDHG12-4
</materialsCitation>
;
<materialsCitation id="4FB674D9F8201CF7662459489C40FBD2" collectingDate="2017-05" collectorName="Cui, LY" country="China" latitude="25.1575" location="Lianfeng Town" longLatPrecision="21" longitude="117.030556" municipality="Longyan City" specimenCount="1" stateProvince="Fujian Province">
<collectingCountry id="24035C440DA570ABA90942A0A4DACAFE" name="China">China</collectingCountry>
,
<collectingRegion id="C11B0DF97265A7509DE1175E9148B840" country="China" name="Fujian">Fujian Province</collectingRegion>
,
<collectingMunicipality id="096D29FC20F2E0C5B91E346E1FC3CBD1">Longyan City</collectingMunicipality>
,
<location id="4117F3B8C3BD155C0EBE28994BBC3955" LSID="urn:lsid:plazi:treatment:6214D26A572553219BB3C5AA4938E345:4117F3B8C3BD155C0EBE28994BBC3955" country="China" latitude="25.1575" longLatPrecision="21" longitude="117.030556" municipality="Longyan City" name="Lianfeng Town" stateProvince="Fujian Province">Lianfeng Town</location>
,
<geoCoordinate id="55B3F3050FB7B587F7A14AA56A7B97AD" degrees="25" direction="north" minutes="09" orientation="latitude" precision="15" seconds="27" value="25.1575">25°09'27&quot;N</geoCoordinate>
,
<geoCoordinate id="6D87AE6EABA961F3C117224A1548EFCE" degrees="117" direction="east" minutes="01" orientation="longitude" precision="15" seconds="50" value="117.030556">117°01'50&quot;E</geoCoordinate>
, isolated from leaf spots of
<taxonomicName id="466ED9C0D9EDA28330BD46B3B91C8F19" authorityName="González-Sponga" authorityYear="1987" lsidName="C. lanceolata" pageId="0" pageNumber="1" rank="species" species="lanceolata">
<emphasis id="3C490650262FAC95DB20F1746716C89D" italics="true" pageId="0" pageNumber="1">C. lanceolata</emphasis>
</taxonomicName>
,
<collectingDate id="B3ACEC1CC9505DF57F502ABFADE52D3E" value="2017-05">May 2017</collectingDate>
, Wen-Li
<collectorName id="829882CB8B1AE8F6CC1A36E4D25C4C1C">Cui, LY</collectorName>
15
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="1A6B9F56BEB956ED6FAD2A2245A31DE3" pageId="0" pageNumber="1" type="notes">
<paragraph id="890C4866D90C10A943259DB68731C288" pageId="0" pageNumber="1">Notes.</paragraph>
<paragraph id="DAF2DCAAC560A017B4469E1450F7AE73" pageId="0" pageNumber="1">
The isolates of
<taxonomicName id="98F4930EBA9B4479CEAD0221D810661C" lsidName="A. shandongensis" pageId="0" pageNumber="1" rank="species" species="shandongensis">
<emphasis id="AECF5870C319A760AB0DD83D76C7D760" italics="true" pageId="0" pageNumber="1">A. shandongensis</emphasis>
</taxonomicName>
were phylogenetically close to
<taxonomicName id="CB2991BECAE01D22DD1A96B12F563F9D" lsidName="A. kunyuensis" pageId="0" pageNumber="1" rank="species" species="kunyuensis">
<emphasis id="5B9BEE7A35ED3006E74C853C6827F230" italics="true" pageId="0" pageNumber="1">A. kunyuensis</emphasis>
</taxonomicName>
(this study, XXG21),
<taxonomicName id="30011C9678B1020FE5387EC4480AAF5F" lsidName="A. hunanensis" pageId="0" pageNumber="1" rank="species" species="hunanensis">
<emphasis id="2763EF94A761440F7D7AAC9FCB1500F7" italics="true" pageId="0" pageNumber="1">A. hunanensis</emphasis>
</taxonomicName>
(this study, HN43-10-2),
<taxonomicName id="75B2900F69AA5087D034E0F901CFE883" lsidName="A. longqiaoensis" pageId="0" pageNumber="1" rank="species" species="longqiaoensis">
<emphasis id="E2A86CF88A4F000D8F36FA34A420D0D1" italics="true" pageId="0" pageNumber="1">A. longqiaoensis</emphasis>
</taxonomicName>
(this study, HN43-14),
<taxonomicName id="26286344352921E1D8CE0E95B7C8A0E1" lsidName="A. vaccinii" pageId="0" pageNumber="1" rank="species" species="vaccinii">
<emphasis id="C1B13AB1596727F160ACCE3927231435" italics="true" pageId="0" pageNumber="1">A. vaccinii</emphasis>
</taxonomicName>
(ex-type, CBS 118818),
<taxonomicName id="BC911C4B4AA4086B36F0237FFA0B728E" lsidName="A. platycodonis" pageId="0" pageNumber="1" rank="species" species="platycodonis">
<emphasis id="8BC75AB88D4418AEF9F80018BC76853F" italics="true" pageId="0" pageNumber="1">A. platycodonis</emphasis>
</taxonomicName>
(ex-type, CBS 121348),
<taxonomicName id="A3D7798F68F8895444592B39E813CA69" lsidName="A. rhadina" pageId="0" pageNumber="1" rank="species" species="rhadina">
<emphasis id="937CFD0C037BD6E72186861E8721F4F7" italics="true" pageId="0" pageNumber="1">A. rhadina</emphasis>
</taxonomicName>
(ex-type, CBS 595.93),
<taxonomicName id="9E27502B26BDD3979A3B4AD8AC2EE3A5" lsidName="A. citriarbusti" pageId="0" pageNumber="1" rank="species" species="citriarbusti">
<emphasis id="F7EA94CE56AFBA992F778235EC0EC7A1" italics="true" pageId="0" pageNumber="1">A. citriarbusti</emphasis>
</taxonomicName>
(ex-type, CBS 102598) and
<taxonomicName id="15719F450B9733E6A7DD700197F65050" lsidName="A. tomaticola" pageId="0" pageNumber="1" rank="species" species="tomaticola">
<emphasis id="AEF6825AA7A437C339FE33A39A3C5E02" italics="true" pageId="0" pageNumber="1">A. tomaticola</emphasis>
</taxonomicName>
(ex-type, CBS 118814) (Fig.
<figureCitation id="D892D261574D44121AC94DBE93ECEBAA" captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Splitgraphs showing the results of the pairwise homoplasy index (PHI) test of newly described taxa and closely-related species using both LogDet transformation and splits decomposition A the PHI of Alternaria xinyangensis sp. nov. and A. dongshanqiaoensis sp. nov. with their phylogenetically related isolates or species B the PHI of A. shandongensis sp. nov., A. kunyuensis sp. nov., A. hunanensis sp. nov. and A. longqiaoensis sp. nov. with their phylogenetically related isolates or species C the PHI of A. cunninghamiicola sp. nov. with their phylogenetically-related isolates or species. PHI test value (Φw) &lt;0.05 indicate significant recombination within a dataset. * indicates strains of this study. T indicates the ex-type strains, ET indicates the ex-epitype strains, HT indicates the ex-holotype strains." figureDoi="10.3897/mycokeys.101.115370.figure2" httpUri="https://binary.pensoft.net/fig/962734" pageId="0" pageNumber="1">2</figureCitation>
). Between
<taxonomicName id="87174AFE63B7D463A4D6A9C4785DE1DD" lsidName="A. shandongensis" pageId="0" pageNumber="1" rank="species" species="shandongensis">
<emphasis id="D4EB5865BA86C592423E04E361BD8EE1" italics="true" pageId="0" pageNumber="1">A. shandongensis</emphasis>
</taxonomicName>
isolates and
<taxonomicName id="344F1987D552F8CBBFC854C1396F5B8B" lsidName="A. kunyuensis" pageId="0" pageNumber="1" rank="species" species="kunyuensis">
<emphasis id="AB925CD0AD3DE074ACB8292F03032CBE" italics="true" pageId="0" pageNumber="1">A. kunyuensis</emphasis>
</taxonomicName>
XXG21, there were 1/453 differences in Alt a1, 2/499 in GAPDH, 1/664 in OPA10-2, 5/757 in RPB2, 1/996 in SSU and 3/293 in TEF1. Between
<taxonomicName id="EE832D535819E337793DEA9582DD58FD" lsidName="A. shandongensis" pageId="0" pageNumber="1" rank="species" species="shandongensis">
<emphasis id="77AB2FE4006EC3DB9A9DF010B7E4F5C8" italics="true" pageId="0" pageNumber="1">A. shandongensis</emphasis>
</taxonomicName>
isolates and
<taxonomicName id="616B9D58916BC17E295500FF043743C7" lsidName="A. hunanensis" pageId="0" pageNumber="1" rank="species" species="hunanensis">
<emphasis id="1B4ADE70D121FE72A9E2232D9F8EB0E7" italics="true" pageId="0" pageNumber="1">A. hunanensis</emphasis>
</taxonomicName>
HN43-10-2, there were 1/453 differences in Alt a1, 2/499 in GAPDH, 1/510 in ITS, 5/401 in endoPG and 1/757 in RPB2. Between
<taxonomicName id="48D334BA1A8ACB7F894331F65E16E977" lsidName="A. shandongensis" pageId="0" pageNumber="1" rank="species" species="shandongensis">
<emphasis id="D9CC9B0FAB04132ED6B60A9A7DB1E991" italics="true" pageId="0" pageNumber="1">A. shandongensis</emphasis>
</taxonomicName>
isolates and
<taxonomicName id="4560555D582D384FD2284DE0A0CB595C" lsidName="A. longqiaoensis" pageId="0" pageNumber="1" rank="species" species="longqiaoensis">
<emphasis id="9E608F8187BBDB33682DB9D81CF04653" italics="true" pageId="0" pageNumber="1">A. longqiaoensis</emphasis>
</taxonomicName>
HN43-14, there were 3/453 differences in Alt a1, 2/499 in GAPDH, 2/510 in ITS, 3/401 in endoPG, 1/757 in RPB2 and 18/996 in SSU. Between
<taxonomicName id="3A502F84830F1060F4FC32D10C116F54" lsidName="A. shandongensis" pageId="0" pageNumber="1" rank="species" species="shandongensis">
<emphasis id="AB866306DF3824C2D5DF61CAD257EC60" italics="true" pageId="0" pageNumber="1">A. shandongensis</emphasis>
</taxonomicName>
isolates and
<taxonomicName id="B43ECE6D61875CAD6B6CC8C28FC28B96" lsidName="A. vaccinii" pageId="0" pageNumber="1" rank="species" species="vaccinii">
<emphasis id="3391F5B749050F23268727B5A1C77016" italics="true" pageId="0" pageNumber="1">A. vaccinii</emphasis>
</taxonomicName>
CBS 118818 (ex-type), there were 5/453 differences in Alt a1, 4/499 in GAPDH, 3/510 in ITS, 4/401 in endoPG and 1/757 in RPB2. Between
<taxonomicName id="D8E179E5321432E321BEA2CB8D83F776" lsidName="A. shandongensis" pageId="0" pageNumber="1" rank="species" species="shandongensis">
<emphasis id="5A2AD0E12E9BA38A47AB0136836ED28A" italics="true" pageId="0" pageNumber="1">A. shandongensis</emphasis>
</taxonomicName>
isolates and
<taxonomicName id="5FD3ECE546F31E8AF9B1C55C5B7D7739" lsidName="A. platycodonis" pageId="0" pageNumber="1" rank="species" species="platycodonis">
<emphasis id="1ED2CC5F0EB52B09DE37153A4A350C14" italics="true" pageId="0" pageNumber="1">A. platycodonis</emphasis>
</taxonomicName>
CBS 121348 (ex-type), there were 2/453 differences in Alt a1, 4/499 in GAPDH, 3/510 in ITS, 3/401 in endoPG and 1/757 in RPB2. Between
<taxonomicName id="144DE12FD2AE67326660117A080B88D5" lsidName="A. shandongensis" pageId="0" pageNumber="1" rank="species" species="shandongensis">
<emphasis id="B06068F3D19EC17310262200AB32A3D4" italics="true" pageId="0" pageNumber="1">A. shandongensis</emphasis>
</taxonomicName>
isolates and
<taxonomicName id="5FEE6FE265B2B39BA127D4F6DE6CCFC2" lsidName="A. rhadina" pageId="0" pageNumber="1" rank="species" species="rhadina">
<emphasis id="A98A5FA0AE86A54AC71D6809E61ED441" italics="true" pageId="0" pageNumber="1">A. rhadina</emphasis>
</taxonomicName>
CBS 595.93 (ex-type), there were 2/453 differences in Alt a1, 4/499 in GAPDH, 3/510 in ITS, 3/401 in endoPG and 1/757 in RPB2. Between
<taxonomicName id="5E78C7189CDC89DDF77DFFAA2C772DC0" lsidName="A. shandongensis" pageId="0" pageNumber="1" rank="species" species="shandongensis">
<emphasis id="B663E60621D1C4A6EC159A198EC3BCAF" italics="true" pageId="0" pageNumber="1">A. shandongensis</emphasis>
</taxonomicName>
isolates and
<taxonomicName id="FA98B47F810E2C97B1E17DB0C94B08A4" lsidName="A. citriarbusti" pageId="0" pageNumber="1" rank="species" species="citriarbusti">
<emphasis id="22DC27B9746F07221BF65230F76E1FDE" italics="true" pageId="0" pageNumber="1">A. citriarbusti</emphasis>
</taxonomicName>
CBS 102598 (ex-type), there were 2/453 differences in Alt a1, 2/499 in GAPDH, 3/510 in ITS, 3/401 in endoPG and 1/757 in RPB2. Between
<taxonomicName id="93A484F517C1551513B2369EDD003D1E" lsidName="A. shandongensis" pageId="0" pageNumber="1" rank="species" species="shandongensis">
<emphasis id="D247749F5426D4C6553B8B80BC642F7F" italics="true" pageId="0" pageNumber="1">A. shandongensis</emphasis>
</taxonomicName>
isolates and
<taxonomicName id="AC5CB91DA4984A7BF41631BF695F3E88" lsidName="A. tomaticola" pageId="0" pageNumber="1" rank="species" species="tomaticola">
<emphasis id="1FDCA445FCEA59ED1E96A16CF053E4C0" italics="true" pageId="0" pageNumber="1">A. tomaticola</emphasis>
</taxonomicName>
CBS 118814 (ex-type), there were 4/453 differences in Alt a1, 2/499 in GAPDH, 3/510 in ITS, 3/401 in endoPG and 1/757 in RPB2. The PHI analysis showed that there was no significant recombination between
<taxonomicName id="22B10EFE1BEED5EF6FDC5F4D138CE3AD" lsidName="A. shandongensis" pageId="0" pageNumber="1" rank="species" species="shandongensis">
<emphasis id="28362E3D732D0833A691537F69BF65D5" italics="true" pageId="0" pageNumber="1">A. shandongensis</emphasis>
</taxonomicName>
isolates and its related species (Φw = 0.3502) (Fig.
<figureCitation id="FBD877754F30D45E5C2E4E66B17EF885" captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Splitgraphs showing the results of the pairwise homoplasy index (PHI) test of newly described taxa and closely-related species using both LogDet transformation and splits decomposition A the PHI of Alternaria xinyangensis sp. nov. and A. dongshanqiaoensis sp. nov. with their phylogenetically related isolates or species B the PHI of A. shandongensis sp. nov., A. kunyuensis sp. nov., A. hunanensis sp. nov. and A. longqiaoensis sp. nov. with their phylogenetically related isolates or species C the PHI of A. cunninghamiicola sp. nov. with their phylogenetically-related isolates or species. PHI test value (Φw) &lt;0.05 indicate significant recombination within a dataset. * indicates strains of this study. T indicates the ex-type strains, ET indicates the ex-epitype strains, HT indicates the ex-holotype strains." figureDoi="10.3897/mycokeys.101.115370.figure2" httpUri="https://binary.pensoft.net/fig/962734" pageId="0" pageNumber="1">2B</figureCitation>
). Distinguishing characteristics of this new species and their related species of
<taxonomicName id="E10B370A13A99B74F218D1FE6DCF2436" class="Dothideomycetes" family="Moraceae" genus="Alternaria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Alternaria" order="Pleosporales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="genus">
<emphasis id="7082609B466B93C5345C86ACAEBE6091" italics="true" pageId="0" pageNumber="1">Alternaria</emphasis>
</taxonomicName>
are shown in Table
<tableCitation id="3AAED4959958B147808DB9EE122001B1" captionStart="Table 2" captionStartId="T2" captionText="Table 2. Distinguishing characteristics of the new species and similar known species of Alternaria spp. under growth conditionsa." httpUri="http://table.plazi.org/id/C175884AFAED43FDBFB74450B8D1CF39" pageId="0" pageNumber="1" tableUuid="C175884AFAED43FDBFB74450B8D1CF39">2</tableCitation>
. Morphologically, conidia in chains of the
<taxonomicName id="C282BC557560ED33F7E4D2A81D9D399F" lsidName="A. shandongensis" pageId="0" pageNumber="1" rank="species" species="shandongensis">
<emphasis id="755FD78D159621A4E35CD9A307EDED41" italics="true" pageId="0" pageNumber="1">A. shandongensis</emphasis>
</taxonomicName>
isolates were more than those of
<taxonomicName id="73095B0DE25BE9D9C1EC3DD81EED16A5" lsidName="A. kunyuensis" pageId="0" pageNumber="1" rank="species" species="kunyuensis">
<emphasis id="494EF59BA968A9B70FEFAC9772869998" italics="true" pageId="0" pageNumber="1">A. kunyuensis</emphasis>
</taxonomicName>
XXG21 (9-13 conidia vs. 6-8 conidia),
<taxonomicName id="757C543D99C82881C0C8E418B7CF8037" lsidName="A. hunanensis" pageId="0" pageNumber="1" rank="species" species="hunanensis">
<emphasis id="988BF25A86DA4AD05457D3C2961FCE09" italics="true" pageId="0" pageNumber="1">A. hunanensis</emphasis>
</taxonomicName>
HN43-10-2 (9-13 conidia vs. 3-7 conidia),
<taxonomicName id="4F48EA440BF884EB55C2C15761FF9723" lsidName="A. longqiaoensis" pageId="0" pageNumber="1" rank="species" species="longqiaoensis">
<emphasis id="0847EDBF7F7ADD9600EA2573FD25F96C" italics="true" pageId="0" pageNumber="1">A. longqiaoensis</emphasis>
</taxonomicName>
HN43-14 (9-13 conidia vs. 4-8 conidia),
<taxonomicName id="AC6C81ACB32F1CEAF4FB11681EDB021F" lsidName="A. citriarbusti" pageId="0" pageNumber="1" rank="species" species="citriarbusti">
<emphasis id="247077C1C9FF96B0E3F1A141E48FEBC6" italics="true" pageId="0" pageNumber="1">A. citriarbusti</emphasis>
</taxonomicName>
CBS 102598 (ex-type) (9-13 conidia vs. 5-8 conidia) (
<bibRefCitation id="566DECECCBD405EBAA8CBC4ACC925E14" DOI="https://doi.org/10.1046/j.1439-0507.1999.00531.x" author="Simmons, EG" journalOrPublisher="Mycotaxon" pageId="0" pageNumber="1" pagination="263 - 323" refId="B78" refString="Simmons, EG, 1999. Alternaria themes and variations (226-235): Classification of citrus pathogens. Mycotaxon 70: 263 - 323, DOI: https://doi.org/10.1046/j.1439-0507.1999.00531.x" title="Alternaria themes and variations (226 - 235): Classification of citrus pathogens." url="https://doi.org/10.1046/j.1439-0507.1999.00531.x" volume="70" year="1999">Simmons 1999</bibRefCitation>
) and
<taxonomicName id="4F2E6335980F92893B2428ECDF76771B" lsidName="A. platycodonis" pageId="0" pageNumber="1" rank="species" species="platycodonis">
<emphasis id="AC7E3705BD754AC90088297AAC50E4D3" italics="true" pageId="0" pageNumber="1">A. platycodonis</emphasis>
</taxonomicName>
CBS 121348 (ex-type) (9-13 conidia vs. 8-10 conidia) (
<bibRefCitation id="D8A769C207691CBBDC9A968F1ACF23E4" author="Zhang, TY" journalOrPublisher="Journal of Wuyi University 武夷学院学报" pageId="0" pageNumber="1" refId="B99" refString="Zhang, TY, 2003. Alternaria. Flora Fungorum Sinicorum." title="Alternaria. Flora Fungorum Sinicorum." year="2003">Zhang 2003</bibRefCitation>
). Conidiophores of the
<taxonomicName id="6AFB83846104AA40E3554D72D0FDEC74" lsidName="A. shandongensis" pageId="0" pageNumber="1" rank="species" species="shandongensis">
<emphasis id="E70EF98E43D300922825DB1961C143AA" italics="true" pageId="0" pageNumber="1">A. shandongensis</emphasis>
</taxonomicName>
isolates were significantly shorter than those of
<taxonomicName id="07B5C56573FA768D35D4084147550DAC" lsidName="A. vaccinii" pageId="0" pageNumber="1" rank="species" species="vaccinii">
<emphasis id="075558BBA055C4419853A90D09E9408B" italics="true" pageId="0" pageNumber="1">A. vaccinii</emphasis>
</taxonomicName>
CBS 118818 (ex-type) (23.6-51.1
<normalizedToken id="CFD0CDAB73B8C738A003F5C540DD141F" originalValue="×">x</normalizedToken>
3.4-4.3
<normalizedToken id="245096464AD5B250E14E2E5FEFBF3F19" originalValue="μm">μm</normalizedToken>
vs. 100-200
<normalizedToken id="2C91B198A82BC2F8C94C2956A8BB773B" originalValue="×">x</normalizedToken>
3-4
<normalizedToken id="84E23172B5E6D9F12B201709BAD9607F" originalValue="μm">μm</normalizedToken>
) (
<bibRefCitation id="A4C724CAF8570B8BCB9DB0B2FC629423" author="Simmons, EG" journalOrPublisher="Mycotaxon" pageId="0" pageNumber="1" refId="B79" refString="Simmons, EG, 2007. Alternaria: An Identification Manual. CBS Biodiversity Series, 775 pp." title="Alternaria: An Identification Manual. CBS Biodiversity Series, 775 pp." year="2007">Simmons 2007</bibRefCitation>
),
<taxonomicName id="6B464AB17815E7EA0BE968FBA2F6EC96" lsidName="A. rhadina" pageId="0" pageNumber="1" rank="species" species="rhadina">
<emphasis id="A6453751566B3285046CEB05C799FFF7" italics="true" pageId="0" pageNumber="1">A. rhadina</emphasis>
</taxonomicName>
CBS 595.93 (ex-type) (23.6-51.1
<normalizedToken id="563E24A248818A2AA885219905EAC9E4" originalValue="×">x</normalizedToken>
3.4-4.3
<normalizedToken id="9B5955B6902E94CC1B4AE9FD5180287B" originalValue="μm">μm</normalizedToken>
vs. 60-110
<normalizedToken id="E10746AD7DBD547DBE30E8FFF27FB4B3" originalValue="×">x</normalizedToken>
3-4
<normalizedToken id="B09802E1025981B569CE491DC9C80864" originalValue="μm">μm</normalizedToken>
) (
<bibRefCitation id="A5A31871E3AC120C8B20395D5B48F1EC" author="Simmons, EG" journalOrPublisher="Mycotaxon" pageId="0" pageNumber="1" pagination="91 - 107" refId="B76" refString="Simmons, EG, 1993. Alternaria themes and variations (63-72). Mycotaxon 48: 91 - 107" title="Alternaria themes and variations (63 - 72)." volume="48" year="1993">Simmons 1993</bibRefCitation>
),
<taxonomicName id="AD8177E5391DA481AA829246D0E42134" lsidName="A. citriarbusti" pageId="0" pageNumber="1" rank="species" species="citriarbusti">
<emphasis id="668CE4D49F1454D7AC529BFFD108D686" italics="true" pageId="0" pageNumber="1">A. citriarbusti</emphasis>
</taxonomicName>
CBS 102598 (ex-type) (23.6-51.1
<normalizedToken id="60DB71CA29B04D556C58B38BC2ADA371" originalValue="×">x</normalizedToken>
3.4-4.3
<normalizedToken id="20D2CE7EAE4C89EDBD6ED7C85C1FEBF6" originalValue="μm">μm</normalizedToken>
vs. 200
<normalizedToken id="AD55C8EF103E7F5891532A8E73C4166A" originalValue="×">x</normalizedToken>
5
<normalizedToken id="492B9F5D66650F8F993676017E70E11F" originalValue="μm">μm</normalizedToken>
) (
<bibRefCitation id="83B57599E909E834060DB39129DB8CD2" DOI="https://doi.org/10.1046/j.1439-0507.1999.00531.x" author="Simmons, EG" journalOrPublisher="Mycotaxon" pageId="0" pageNumber="1" pagination="263 - 323" refId="B78" refString="Simmons, EG, 1999. Alternaria themes and variations (226-235): Classification of citrus pathogens. Mycotaxon 70: 263 - 323, DOI: https://doi.org/10.1046/j.1439-0507.1999.00531.x" title="Alternaria themes and variations (226 - 235): Classification of citrus pathogens." url="https://doi.org/10.1046/j.1439-0507.1999.00531.x" volume="70" year="1999">Simmons 1999</bibRefCitation>
) and
<taxonomicName id="7F4086101EC5BDA971A42873A5748D4B" lsidName="A. tomaticola" pageId="0" pageNumber="1" rank="species" species="tomaticola">
<emphasis id="A1B4678F00E644EF5092D942A15176DC" italics="true" pageId="0" pageNumber="1">A. tomaticola</emphasis>
</taxonomicName>
CBS 118814 (ex-type) (23.6-51.1
<normalizedToken id="3D522F871AE106F690605A7395AD35FC" originalValue="×">x</normalizedToken>
3.4-4.3
<normalizedToken id="5D81986E838BCD6C1077CC2046439483" originalValue="μm">μm</normalizedToken>
vs. 50-80
<normalizedToken id="CB2386DCCF7B30E30932EFA2D7F69D50" originalValue="×">x</normalizedToken>
3-5
<normalizedToken id="774CAFEE12FBAD5FA21E7424DAE937BA" originalValue="μm">μm</normalizedToken>
) (
<bibRefCitation id="A0C38365426F4589C1B7F89305232193" author="Simmons, EG" journalOrPublisher="Mycotaxon" pageId="0" pageNumber="1" refId="B79" refString="Simmons, EG, 2007. Alternaria: An Identification Manual. CBS Biodiversity Series, 775 pp." title="Alternaria: An Identification Manual. CBS Biodiversity Series, 775 pp." year="2007">Simmons 2007</bibRefCitation>
). In conclusion, the phylogenetic and morphological evidence supports this fungus as being a new species within the
<taxonomicName id="644E7F7EF1705562CDBF9DDC1C10964C" class="Dothideomycetes" family="Moraceae" genus="Alternaria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Alternaria alternata" order="Pleosporales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="alternata">
<emphasis id="242E6540A3A0A6515638B429C76DA0E5" italics="true" pageId="0" pageNumber="1">Alternaria alternata</emphasis>
</taxonomicName>
species complex.
</paragraph>
</subSubSection>
</treatment>
</document>