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<mods:title id="5FD83CB4BF60424DE5F8E5A000530932">New reports, phylogenetic analysis, and a key to Lactarius Pers. in the Greater Yellowstone Ecosystem informed by molecular data</mods:title>
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<subSubSection id="5CD54DCEC0E14079693A9B44D8F1918B" pageId="8" pageNumber="9" type="multiple">
<paragraph id="8F892B4BF1EB9A00B75D9E673B750529" pageId="8" pageNumber="9">Taxon classification Fungi Russulales Russulaceae</paragraph>
</subSubSection>
<subSubSection id="337B90DBAE658AE1175A2ABFDD603EC5" pageId="8" pageNumber="9" type="nomenclature">
<paragraph id="EBF52997E1E9B895DA785F8229F1885B" pageId="8" pageNumber="9">
1.
<taxonomicName id="852796DC2A0CD51BF65FE122B1094089" ID-CoL="5PMG3" authority="Hesler &amp; A. H. Sm." class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" infraspecific-rank="v." kingdom="Fungi" lsidName="Lactarius alpinus var. mitis" order="Russulales" pageId="8" pageNumber="9" phylum="Basidiomycota" rank="variety" species="alpinus" variety="mitis">Lactarius alpinus v. mitis Hesler &amp; A.H. Sm.</taxonomicName>
Figure 3
</paragraph>
</subSubSection>
<subSubSection id="D1D4EE01B1A85F0B57AFDFAA11AE4470" pageId="8" pageNumber="9" type="description">
<paragraph id="D291DBAFCE1D14C422FBA60407E7E619" pageId="8" pageNumber="9">Description.</paragraph>
<paragraph id="484B74C201779B71C08E9F342BFCE5F3" pageId="8" pageNumber="9">
Pileus 8-20 mm in diameter (to 80 mm in
<bibRefCitation id="EEA798AA60ADCE0776503429731C480C" author="Hesler, LR" journalOrPublisher="University of Michigan Press" pageId="50" pageNumber="51" pagination="1 - 841" title="North American species of Lactarius" year="1979">Hesler and Smith 1979</bibRefCitation>
), plano-convex to infundibuliform,
<normalizedToken id="881C9ACEB21C895204D76F9124F3A811" originalValue="±">+/-</normalizedToken>
papillate, dry, finely rimulose to minutely squamulose, red-brown to orange-brown to pinkish cinnamon; margin finely crenulate, incurved when young, becoming straight to wavy in age. Lamellae adnate to subdecurrent, subdistant to crowded, cream to creamy tan, discoloring slightly darker where damaged. Stipe 8-23
<normalizedToken id="DE8BC94BB599409F1CA5C2B312264DAB" originalValue="×">x</normalizedToken>
5-8 mm (30-50
<normalizedToken id="C9694A7274B3B8F0195E1DBAA4627785" originalValue="×">x</normalizedToken>
10-15 mm in
<bibRefCitation id="32B3054012E8638889873187A47BD70D" author="Hesler, LR" journalOrPublisher="University of Michigan Press" pageId="50" pageNumber="51" pagination="1 - 841" title="North American species of Lactarius" year="1979">Hesler and Smith 1979</bibRefCitation>
), equal to slightly clavate, smooth, dry, pale creamy yellow to creamy tan, solid, becoming hollow. Context concolorous with pileus. Latex scarce, watery, white, unchanging. Odor mild. Taste mild.
</paragraph>
<paragraph id="61F82D94519B0622F3D0AF7213F621FA" pageId="8" pageNumber="9">
Basidiospores 7-9.5
<normalizedToken id="F1F36B0F3A072678256BB31E7AB89F18" originalValue="×">x</normalizedToken>
5-7.5
<normalizedToken id="36F48D62CB0F0BCA1F4AF2DC2997B80E" originalValue="µm">µm</normalizedToken>
, Q = 1.3-1.5, ellipsoid; ornamentation forming a broken to partial reticulum. Pleuromacrocystidia 60-100
<normalizedToken id="D54B2CB45053F0D38D37B77A6256A868" originalValue="×">x</normalizedToken>
6-10
<normalizedToken id="06E3913FBB0B4DC404DDE2A8C496F4A7" originalValue="µm">µm</normalizedToken>
, numerous, strongly projecting, mucronate to fusoid; apex acute to moniliform. Cheilomacrocystidia 50-80
<normalizedToken id="93BB0C9FEBCF8CD913CF9F125FA6DD17" originalValue="×">x</normalizedToken>
6-10
<normalizedToken id="3740307627368EB56C830B3E1491D964" originalValue="µm">µm</normalizedToken>
, numerous, strongly projecting, mucronate to fusoid; apex acute to moniliform.
</paragraph>
</subSubSection>
<subSubSection id="F89697E5E4F6B0C485A8B2FF8F8A9ADB" pageId="8" pageNumber="9" type="ecology">
<paragraph id="FB7CF7D7D9A6D824038E5E68DCA6FB5E" pageId="8" pageNumber="9">Ecology and distribution.</paragraph>
<paragraph id="8BB279DD6CEC243A0AB9C4E66247546F" pageId="8" pageNumber="9">
In western and into eastern North America with
<taxonomicName id="1FB9F4C96C0ABF32CA5BB749DCEC6DBD" class="Magnoliopsida" family="Betulaceae" genus="Alnus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Alnus" order="Fagales" pageId="8" pageNumber="9" phylum="Tracheophyta" rank="genus">Alnus</taxonomicName>
. In the GYE only one collection has been made thus far and it was from a riparian area near
<taxonomicName id="6348E1B23E9C6D31D979B754F36B6689" class="Magnoliopsida" family="Betulaceae" genus="Alnus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Alnus incana" order="Fagales" pageId="8" pageNumber="9" phylum="Tracheophyta" rank="species" species="incana">Alnus incana</taxonomicName>
and
<taxonomicName id="89993A681C7F67A6CB5D0FBF09BA8472" class="Magnoliopsida" family="Salicaceae" genus="Populus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Populus trichocarpa" order="Malpighiales" pageId="8" pageNumber="9" phylum="Tracheophyta" rank="species" species="trichocarpa">Populus trichocarpa</taxonomicName>
, late summer. Further sampling under
<taxonomicName id="C093F9232EA410E2367084C6E678BD71" class="Magnoliopsida" family="Betulaceae" genus="Alnus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Alnus" order="Fagales" pageId="8" pageNumber="9" phylum="Tracheophyta" rank="genus">Alnus</taxonomicName>
in the Rocky Mountains is necessary.
</paragraph>
</subSubSection>
<subSubSection id="470F4327838EC5BDAAFA58D4097E0066" pageId="8" pageNumber="9" type="materials_examined">
<paragraph id="312870780A214CA66190309148A6E2EC" pageId="8" pageNumber="9">Specimens examined.</paragraph>
<paragraph id="A14BDE35E63137713D656C4478B0093A" pageId="8" pageNumber="9">
U.S.A. MONTANA: Carbon County, Red Lodge, along Rock Creek, under
<taxonomicName id="E2E79E9FC63D890FF31A89FF49A99F7C" class="Magnoliopsida" family="Betulaceae" genus="Alnus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Alnus incana" order="Fagales" pageId="8" pageNumber="9" phylum="Tracheophyta" rank="species" species="incana">Alnus incana</taxonomicName>
and
<taxonomicName id="AA1AEAF1B9F14C0C7B65C85F2196CF14" class="Magnoliopsida" family="Salicaceae" genus="Populus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Populus trichocarpa" order="Malpighiales" pageId="8" pageNumber="9" phylum="Tracheophyta" rank="species" species="trichocarpa">Populus trichocarpa</taxonomicName>
, 6 Sept 2015, EB161-15 (MONT).
</paragraph>
</subSubSection>
<subSubSection id="F25346A244CF1F9BC0D18FD5C8F0E21D" pageId="9" pageNumber="10" type="discussion">
<paragraph id="76E85DDB731FE6FDA445CC3505C91220" pageId="9" pageNumber="10">
<pageBreakToken id="7E2D41C551B4AA47C7CF7F3B5AF05A77" pageId="9" pageNumber="10" start="start">Discussion</pageBreakToken>
.
</paragraph>
<paragraph id="B8AD70886C7175901680744BED581596" pageId="9" pageNumber="10">
This is the first report of
<taxonomicName id="2D6B63A154637BB42E66E3C30705091B" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" infraspecific-rank="v." kingdom="Fungi" lsidName="Lactarius alpinus var. mitis" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="variety" species="alpinus" variety="mitis">Lactarius alpinus v. mitis</taxonomicName>
from the GYE. According to
<bibRefCitation id="3ED771EB7E4E281844A41877DABA5F36" author="Hesler, LR" journalOrPublisher="University of Michigan Press" pageId="50" pageNumber="51" pagination="1 - 841" title="North American species of Lactarius" year="1979">Hesler and Smith (1979)</bibRefCitation>
,
<taxonomicName id="55EEC0096879FACA5BC1D66831BB5099" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" infraspecific-rank="v." kingdom="Fungi" lsidName="Lactarius alpinus var. mitis" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="variety" species="alpinus" variety="mitis">Lactarius alpinus v. mitis</taxonomicName>
has duller pileus and lamellae colors than
<taxonomicName id="C63DC140494F9B689735A63892103D34" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius alpinus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="alpinus">Lactarius alpinus</taxonomicName>
v.
<taxonomicName id="CD82FC8F5638EC48F08827EC12CAF1C6" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius alpinus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="alpinus">alpinus</taxonomicName>
Peck, which typically develops bright yellow to apricot colors; variety
<taxonomicName id="A7CE63372466E14779D31AB0EEF5081A" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius mitis var. mitis" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="variety" species="mitis" variety="mitis">mitis</taxonomicName>
also has longer and more conspicuous macrocystidia and a mild rather than slowly acrid taste. The collection described here likely did not reach full maturity, and the basidiomes are smaller than reported in
<bibRefCitation id="DD077464E5431A5E01C37D8C4346D1FB" author="Hesler, LR" journalOrPublisher="University of Michigan Press" pageId="50" pageNumber="51" pagination="1 - 841" title="North American species of Lactarius" year="1979">Hesler and Smith (1979)</bibRefCitation>
. Phylogenetic analyses including material of
<taxonomicName id="50B85B55D688153835633835EADA940C" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius alpinus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="alpinus">Lactarius alpinus</taxonomicName>
v.
<taxonomicName id="9912496E70AEDAA5DF0FE5A1FB6F2A9C" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius alpinus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="alpinus">alpinus</taxonomicName>
from near the type locality suggest that
<taxonomicName id="C9F4424FAD059CE997426FE7AAFE5F8E" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" infraspecific-rank="v." kingdom="Fungi" lsidName="Lactarius alpinus var. mitis" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="variety" species="alpinus" variety="mitis">Lactarius alpinus v. mitis</taxonomicName>
and
<taxonomicName id="EB2B3B2487F51904204BC1AD68667B5E" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius alpinus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="alpinus">Lactarius alpinus</taxonomicName>
v.
<taxonomicName id="A8684755B8279B9001D72A5BC146FDB9" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius alpinus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="alpinus">alpinus</taxonomicName>
are distinct species (Figure 2A, 2C), however a more in depth analysis incorporating type material of at least
<taxonomicName id="B1F6CA4B4A1FCC7A77184A87138ECE76" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" infraspecific-rank="v." kingdom="Fungi" lsidName="Lactarius alpinus var. mitis" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="variety" species="alpinus" variety="mitis">Lactarius alpinus v. mitis</taxonomicName>
should be carried out before making this determination final. For an interesting discussion on
<taxonomicName id="F413E68E28FCCA9E58A6A10DA8F874B1" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius alpinus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="alpinus">Lactarius alpinus</taxonomicName>
see
<bibRefCitation id="199A58405815EDF0859172CD9736D5E4" author="Nuytinck, J" journalOrPublisher="Omphalina" pageId="51" pageNumber="52" pagination="12 - 14" title="Lactariusalpinus." volume="7" year="2016">Nuytinck and Voitk (2016)</bibRefCitation>
and
<bibRefCitation id="3B653E3B6C13448900A5E7CAF00AA257" author="Rochet, J" journalOrPublisher="BMC Evolutionary Biology" pageId="51" pageNumber="52" pagination="40 - 50" title="Comparative phylogenies and host specialization in the alder ectomycorrhizal fungi Alnicola, Alpova and Lactarius (Basidiomycota) in Europe." url="10.1186/1471-2148-11-40" volume="11" year="2011">Rochet et al. (2011)</bibRefCitation>
.
</paragraph>
<paragraph id="C990675F4FDA2A597274A2081163B264" pageId="9" pageNumber="10">
While
<taxonomicName id="C772B39745EB42F5C3207D5E5EF3337D" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" infraspecific-rank="v." kingdom="Fungi" lsidName="Lactarius alpinus var. mitis" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="variety" species="alpinus" variety="mitis">Lactarius alpinus v. mitis</taxonomicName>
appears to be quite distantly related to
<taxonomicName id="EDD741230BBC7BBC723BD7B41429B0A2" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius alpinus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="alpinus">Lactarius alpinus</taxonomicName>
v.
<taxonomicName id="A1C934301073B83CF8C5AE040EF6F7FA" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius alpinus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="alpinus">alpinus</taxonomicName>
, it is very closely related to the European
<taxonomicName id="9732013868038CCDF6783B3D72D8D95D" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius lilacinus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="lilacinus">Lactarius lilacinus</taxonomicName>
(Lasch) Fr., the recently described Mexican
<taxonomicName id="E5923953EF2ACEAE75FB8CA02D523B77" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius cuspidoaurantiacus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="cuspidoaurantiacus">Lactarius cuspidoaurantiacus</taxonomicName>
Montoya, Bandala &amp; Garay-Serr., and to a lesser extent
<taxonomicName id="43AC46C87BE8EDE941BDA2B626E20D4F" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius lepidotus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="lepidotus">Lactarius lepidotus</taxonomicName>
Hesler &amp; A.H. Sm., all of which are also associated with
<taxonomicName id="453F3CB90053F1623CC83FDB0121FADE" class="Magnoliopsida" family="Betulaceae" genus="Alnus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Alnus" order="Fagales" pageId="9" pageNumber="10" phylum="Tracheophyta" rank="genus">Alnus</taxonomicName>
(Figure 2A).
<taxonomicName id="84834026D28EC5C6DD0BDA69FA35EBDC" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius lilacinus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="lilacinus">Lactarius lilacinus</taxonomicName>
is typically more pink to violet in color and has a fruity smell.
<taxonomicName id="3F732520818D88A15D5170A822C06F8A" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius cuspidoaurantiacus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="cuspidoaurantiacus">Lactarius cuspidoaurantiacus</taxonomicName>
appears to have more yellow-orange basidiomes, more orange colored, distant lamellae, and a
<taxonomicName id="83F94C3DE39A9BABA89332301364CC41" class="Magnoliopsida" family="Geraniaceae" genus="Pelargonium" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Pelargonium" order="Geraniales" pageId="9" pageNumber="10" phylum="Tracheophyta" rank="genus">Pelargonium</taxonomicName>
-like odor (
<bibRefCitation id="0337C7E634276E3BEEFB675D6EE777FD" author="Montoya, L" journalOrPublisher="Mycologia" pageId="51" pageNumber="52" pagination="949 - 962" title="Two new species of Lactarius associated with Alnusacuminatasubsp. arguta in Mexico." url="10.3852/14-006" volume="106" year="2014">Montoya et al. 2014</bibRefCitation>
).
<taxonomicName id="9218C41C852501340970EC5304E8B065" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius lepidotus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="lepidotus">Lactarius lepidotus</taxonomicName>
has a more gray-brown to beige pileus. Neither
<taxonomicName id="75B0511D24ED7D143634D7E4655C6D6C" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius lilacinus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="lilacinus">Lactarius lilacinus</taxonomicName>
,
<taxonomicName id="32FFB26A921D96AA02F7D80D37AC0289" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius cuspidoaurantiacus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="cuspidoaurantiacus">Lactarius cuspidoaurantiacus</taxonomicName>
or
<taxonomicName id="FB12B21D9D8F832AA63052C238EC462C" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lactarius lepidotus" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="species" species="lepidotus">Lactarius lepidotus</taxonomicName>
have been reported from the Rocky Mountains.
</paragraph>
<caption id="D73387737D54EF43DEBB09C40A540552" pageId="9" pageNumber="10">
<paragraph id="A9E42F65A665F4851EE38CC86AD3FA5D" pageId="9" pageNumber="10">
Figure 3.
<taxonomicName id="5253D011D0094E677F2A960FCD7A7936" class="Agaricomycetes" family="Russulaceae" genus="Lactarius" higherTaxonomySource="CoL" infraspecific-rank="v." kingdom="Fungi" lsidName="Lactarius alpinus var. mitis" order="Russulales" pageId="9" pageNumber="10" phylum="Basidiomycota" rank="variety" species="alpinus" variety="mitis">Lactarius alpinus v. mitis</taxonomicName>
. Top collection EB161-15 under
<taxonomicName id="463567D3D3975BABCC4586EB83F10AC3" class="Magnoliopsida" family="Betulaceae" genus="Alnus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Alnus incana" order="Fagales" pageId="9" pageNumber="10" phylum="Tracheophyta" rank="species" species="incana">Alnus incana</taxonomicName>
and
<taxonomicName id="4B65D57C7511C9EC2A51941A6008931F" class="Magnoliopsida" family="Salicaceae" genus="Populus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Populus trichocarpa" order="Malpighiales" pageId="9" pageNumber="10" phylum="Tracheophyta" rank="species" species="trichocarpa">Populus trichocarpa</taxonomicName>
, Red Lodge, Montana, USA. Bottom collection EB516-15 under
<taxonomicName id="F2B642E619FF1806CED9D230718FDB4C" class="Magnoliopsida" family="Betulaceae" genus="Alnus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Alnus rubra" order="Fagales" pageId="9" pageNumber="10" phylum="Tracheophyta" rank="species" species="rubra">Alnus rubra</taxonomicName>
, western Oregon, USA. Scale bars: 2 cm. Photos by E. Barge.
</paragraph>
</caption>
</subSubSection>
</treatment>
</document>