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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.97.102653" ID-Pensoft-Pub="1314-4049-97-1" ID-Pensoft-UUID="B1CB07CBA9615AC78EBABF12E18FB465" ModsDocID="1314-4049-97-1" checkinTime="1682545342214" checkinUser="pensoft" docAuthor="Lin, Lu, Bai, Yukun, Pan, Meng, Tian, Chengming &amp; Fan, Xinlei" docDate="2023" docId="CB6DB2A7D5885F588699D19771F30133" docLanguage="en" docName="MycoKeys 97: 1-19" docOrigin="MycoKeys 97" docPubDate="2023-04-26" docSource="http://dx.doi.org/10.3897/mycokeys.97.102653" docTitle="Dothiorella baihuashanensis L. Lin &amp; X. L. Fan 2023, sp. nov." docType="treatment" docVersion="1" id="B1CB07CBA9615AC78EBABF12E18FB465" lastPageNumber="1" masterDocId="B1CB07CBA9615AC78EBABF12E18FB465" masterDocTitle="Morphology and molecular analyses reveal three new species of Botryosphaeriales isolated from diseased plant branches in China" masterLastPageNumber="19" masterPageNumber="1" pageNumber="1" updateTime="1682545342214" updateUser="pensoft">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Morphology and molecular analyses reveal three new species of Botryosphaeriales isolated from diseased plant branches in China</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Lin, Lu</mods:namePart>
<mods:affiliation>The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Bai, Yukun</mods:namePart>
<mods:affiliation>The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Pan, Meng</mods:namePart>
<mods:affiliation>The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Tian, Chengming</mods:namePart>
<mods:affiliation>The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Fan, Xinlei</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-4946-4442</mods:nameIdentifier>
<mods:affiliation>The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China</mods:affiliation>
<mods:nameIdentifier type="email">xinleifan@bjfu.edu.cn</mods:nameIdentifier>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2023</mods:date>
<mods:detail type="pubDate">
<mods:number>2023-04-26</mods:number>
</mods:detail>
<mods:detail type="volume">
<mods:number>97</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>1</mods:start>
<mods:end>19</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.97.102653</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.97.102653</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-97-1</mods:identifier>
<mods:identifier type="Pensoft-UUID">B1CB07CBA9615AC78EBABF12E18FB465</mods:identifier>
</mods:mods>
<treatment LSID="urn:lsid:plazi:treatment:CB6DB2A7D5885F588699D19771F30133" httpUri="http://treatment.plazi.org/id/CB6DB2A7D5885F588699D19771F30133" lastPageNumber="1" pageId="0" pageNumber="1">
<subSubSection pageId="0" pageNumber="1" type="nomenclature">
<paragraph pageId="0" pageNumber="1">
<taxonomicName LSID="CB6DB2A7-D588-5F58-8699-D19771F30133" authority="L. Lin &amp; X. L. Fan" authorityName="L. Lin &amp; X. L. Fan" authorityYear="2023" class="Dothideomycetes" family="Botryosphaeriaceae" genus="Dothiorella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Dothiorella baihuashanensis" order="Botryosphaeriales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="baihuashanensis" status="sp. nov.">Dothiorella baihuashanensis L. Lin &amp; X.L. Fan</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="1">sp. nov.</taxonomicNameLabel>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="description">
<paragraph pageId="0" pageNumber="1">
<figureCitation captionStart="Figure 5" captionStartId="F5" captionText="Figure 5. Dothiorella baihuashanensis (BJFC CF 20230107) A, B habit of conidiomata on twig C transverse section of a conidioma D longitudinal section through a conidioma E, F conidiogenous cells G conidia H colony on PDA at 14 days I colony on MEA at 14 days. Scale bars: 1 mm (A); 200 µm (B-D); 10 µm (E-G)." figureDoi="10.3897/mycokeys.97.102653.figure5" httpUri="https://binary.pensoft.net/fig/842803" pageId="0" pageNumber="1">Fig. 5</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="etymology">
<paragraph pageId="0" pageNumber="1">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="1">Named after the collection site of the type specimen, Baihuashan Natural Scenic Area in Beijing City.</paragraph>
<caption doi="10.3897/mycokeys.97.102653.figure5" httpUri="https://binary.pensoft.net/fig/842803" pageId="0" pageNumber="1" start="Figure 5" startId="F5">
<paragraph pageId="0" pageNumber="1">
<emphasis bold="true" pageId="0" pageNumber="1">Figure 5.</emphasis>
<taxonomicName authorityName="L. Lin &amp; X. L. Fan" authorityYear="2023" class="Dothideomycetes" family="Botryosphaeriaceae" genus="Dothiorella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Dothiorella baihuashanensis" order="Botryosphaeriales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="baihuashanensis">
<emphasis italics="true" pageId="0" pageNumber="1">Dothiorella baihuashanensis</emphasis>
</taxonomicName>
(BJFC CF20230107)
<emphasis bold="true" pageId="0" pageNumber="1">A, B</emphasis>
habit of conidiomata on twig
<emphasis bold="true" pageId="0" pageNumber="1">C</emphasis>
transverse section of a conidioma
<emphasis bold="true" pageId="0" pageNumber="1">D</emphasis>
longitudinal section through a conidioma
<emphasis bold="true" pageId="0" pageNumber="1">E, F</emphasis>
conidiogenous cells
<emphasis bold="true" pageId="0" pageNumber="1">G</emphasis>
conidia
<emphasis bold="true" pageId="0" pageNumber="1">H</emphasis>
colony on PDA at 14 days
<emphasis bold="true" pageId="0" pageNumber="1">I</emphasis>
colony on MEA at 14 days. Scale bars: 1 mm (
<emphasis bold="true" pageId="0" pageNumber="1">A</emphasis>
); 200
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="1">B-D</emphasis>
); 10
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="1">E-G</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="description">
<paragraph pageId="0" pageNumber="1">Description.</paragraph>
<paragraph pageId="0" pageNumber="1">
Conidiomata pycnidial, superficial or immersed, separate, ovoid, 350-500
<normalizedToken originalValue="µm">µm</normalizedToken>
in diam, occasionally aggregated into botryose clusters. Disc black, 200-300
<normalizedToken originalValue="µm">µm</normalizedToken>
in diam. Ostioles single, central, papillate. Conidiophores reduced to conidiogenous cells. Conidiogenous cells hyaline, holoblastic, cylindrical to subcylindrical or broadly lageniform, 7.5-16.0
<normalizedToken originalValue="×">x</normalizedToken>
3.5-6.5
<normalizedToken originalValue="μm">μm</normalizedToken>
(av.
<normalizedToken originalValue="±">+/-</normalizedToken>
S.D. = 11.7
<normalizedToken originalValue="±">+/-</normalizedToken>
2.2
<normalizedToken originalValue="×">x</normalizedToken>
4.6
<normalizedToken originalValue="±">+/-</normalizedToken>
0.7
<normalizedToken originalValue="μm">μm</normalizedToken>
). Conidia1-septate, hazel to blackish brown, mostly truncate at the base and constricted at the septum or with a thickening at the base of the septum, moderately thick-walled, ovoid or oblong to ellipsoidal, 22.5-35.0
<normalizedToken originalValue="×">x</normalizedToken>
11.0-19.0
<normalizedToken originalValue="μm">μm</normalizedToken>
(av.
<normalizedToken originalValue="±">+/-</normalizedToken>
S.D. = 27.9
<normalizedToken originalValue="±">+/-</normalizedToken>
2.9
<normalizedToken originalValue="×">x</normalizedToken>
14.3
<normalizedToken originalValue="±">+/-</normalizedToken>
2.2
<normalizedToken originalValue="μm">μm</normalizedToken>
).
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="description">
<paragraph pageId="0" pageNumber="1">Culture characters.</paragraph>
<paragraph pageId="0" pageNumber="1">Colonies on PDA spreading, covering a 90 mm plate after 14 days at 25 °C, upper white to pale grey, reverse buff to dark grey. Colonies on MEA spreading, covering a 90 mm plate after 14 days at 25 °C, uniform with appressed aerial mycelium and crenate edge, upper white to pale grey, reverse honey to dark grey.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="materials_examined">
<paragraph pageId="0" pageNumber="1">Materials examined.</paragraph>
<paragraph pageId="0" pageNumber="1">
<materialsCitation collectingDate="2022-08-23" collectorName="Lu Lin, Xinlei Fan" country="China" county="Qingshui County" latitude="39.838375" location="Mentougou District" longLatPrecision="1" longitude="115.57256" municipality="City" specimenCount="1" stateProvince="Beijing" typeStatus="holotype">
<collectingCountry name="China">China</collectingCountry>
,
<collectingRegion country="China" name="Beijing">Beijing</collectingRegion>
<collectingMunicipality>City</collectingMunicipality>
,
<location LSID="urn:lsid:plazi:treatment:CB6DB2A7D5885F588699D19771F30133:D073715F2E625625DCE933BD1AE284F0" country="China" county="Qingshui County" latitude="39.838375" longLatPrecision="1" longitude="115.57256" municipality="City" name="Mentougou District" stateProvince="Beijing">Mentougou District</location>
,
<collectingCounty>Qingshui County</collectingCounty>
,
<location LSID="urn:lsid:plazi:treatment:CB6DB2A7D5885F588699D19771F30133:76FDB1A0A00C8EDA5E7EA6A3E16A9E54" country="China" county="Qingshui County" latitude="39.838375" longLatPrecision="1" longitude="115.57256" municipality="City" name="Baihuashan Natural Scenic Area" stateProvince="Beijing">Baihuashan Natural Scenic Area</location>
,
<geoCoordinate degrees="39" direction="north" minutes="50" orientation="latitude" precision="1" seconds="18.21" value="39.83839">39°50'18.21&quot;N</geoCoordinate>
,
<geoCoordinate degrees="115" direction="east" minutes="34" orientation="longitude" precision="1" seconds="21.13" value="115.57253">115°34'21.13&quot;E</geoCoordinate>
, on dead branches of
<taxonomicName class="Pinopsida" family="Cupressaceae" genus="Juniperus" kingdom="Plantae" lsidName="Juniperus chinensis" order="Pinales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="species" species="chinensis">
<emphasis italics="true" pageId="0" pageNumber="1">Juniperus chinensis</emphasis>
</taxonomicName>
,
<collectingDate value="2022-08-23">23 August 2022</collectingDate>
,
<collectorName>Lu Lin</collectorName>
&amp;
<collectorName>Xinlei Fan</collectorName>
(
<typeStatus>holotype</typeStatus>
BJFC CF20230107, ex-holotype culture CFCC 58549);
<geoCoordinate degrees="39" direction="north" minutes="50" orientation="latitude" precision="1" seconds="18.16" value="39.838375">39°50'18.16&quot;N</geoCoordinate>
,
<geoCoordinate degrees="115" direction="east" minutes="34" orientation="longitude" precision="1" seconds="21.24" value="115.57256">115°34'21.24&quot;E</geoCoordinate>
, on dead branches of
<taxonomicName class="Pinopsida" family="Cupressaceae" genus="Juniperus" kingdom="Plantae" lsidName="Juniperus chinensis" order="Pinales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="species" species="chinensis">
<emphasis italics="true" pageId="0" pageNumber="1">Juniperus chinensis</emphasis>
</taxonomicName>
,
<collectingDate value="2022-08-23">23 August 2022</collectingDate>
,
<collectorName>Lu Lin</collectorName>
&amp;
<collectorName>Xinlei Fan</collectorName>
(
<typeStatus>paratype</typeStatus>
BJFC CF20230108, ex-paratype culture CFCC 58788)
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="notes">
<paragraph pageId="0" pageNumber="1">Notes.</paragraph>
<paragraph pageId="0" pageNumber="1">
The isolates CFCC 58549 and 58788 in this study formed a distinct linage in the phylogenetic trees of each individual gene (ITS,
<emphasis italics="true" pageId="0" pageNumber="1">tef1</emphasis>
-α, and
<emphasis italics="true" pageId="0" pageNumber="1">tub2</emphasis>
) and the combined gene dataset (Fig.
<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Phylogram of Dothiorella resulting from a maximum likelihood analysis based on combined ITS, tef 1 and tub 2 loci. Numbers above the branches indicateML bootstrap values (ML-BS ≥ 70 %) and Bayesian Posterior Probabilities (BPP ≥ 0.9). The tree is rooted with Lasiodiplodia americana CFCC 50065. Ex-type isolates are in bold. Isolates from the present study are marked in blue." figureDoi="10.3897/mycokeys.97.102653.figure2" httpUri="https://binary.pensoft.net/fig/842800" pageId="0" pageNumber="1">2</figureCitation>
). They were isolated from the branches
<taxonomicName class="Pinopsida" family="Cupressaceae" genus="Juniperus" kingdom="Plantae" lsidName="Juniperus chinensis" order="Pinales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="species" species="chinensis">
<emphasis italics="true" pageId="0" pageNumber="1">Juniperus chinensis</emphasis>
</taxonomicName>
.
<taxonomicName class="Dothideomycetes" family="Botryosphaeriaceae" genus="Dothiorella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Dothiorella iberica" order="Botryosphaeriales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="iberica">
<emphasis italics="true" pageId="0" pageNumber="1">Dothiorella iberica</emphasis>
</taxonomicName>
was also recorded to host genus
<taxonomicName authorityName="C.Linnaeus" authorityYear="1753" class="Pinopsida" family="Cupressaceae" genus="Juniperus" kingdom="Plantae" lsidName="Juniperus" order="Pinales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="1">Juniperus</emphasis>
</taxonomicName>
(
<bibRefCitation DOI="https://doi.org/10.1007/s10658-012-0122-2" author="Alves, A" journalOrPublisher="European Journal of Plant Pathology" pageId="0" pageNumber="1" pagination="791 - 804" refId="B1" refString="Alves, A, Barradas, C, Phillips, AJL, Correia, A, 2013. Diversity of Botryosphaeriaceae species associated with conifers in Portugal. European Journal of Plant Pathology 135 (4): 791 - 804, DOI: https://doi.org/10.1007/s10658-012-0122-2" title="Diversity of Botryosphaeriaceae species associated with conifers in Portugal." url="https://doi.org/10.1007/s10658-012-0122-2" volume="135" year="2013">Alves et al. 2013</bibRefCitation>
). However, these two species are not closely related in our phylogenetic analysis.
</paragraph>
</subSubSection>
</treatment>
</document>