Morphology and molecular analyses reveal three new species of Botryosphaeriales isolated from diseased plant branches in China Author Lin, Lu The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China Author Bai, Yukun The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China Author Pan, Meng The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China Author Tian, Chengming The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China Author Fan, Xinlei https://orcid.org/0000-0002-4946-4442 The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China xinleifan@bjfu.edu.cn text MycoKeys 2023 2023-04-26 97 1 19 http://dx.doi.org/10.3897/mycokeys.97.102653 journal article http://dx.doi.org/10.3897/mycokeys.97.102653 1314-4049-97-1 B1CB07CBA9615AC78EBABF12E18FB465 Dothiorella baihuashanensis L. Lin & X.L. Fan sp. nov. Fig. 5 Etymology. Named after the collection site of the type specimen, Baihuashan Natural Scenic Area in Beijing City. Figure 5. Dothiorella baihuashanensis (BJFC CF20230107) A, B habit of conidiomata on twig C transverse section of a conidioma D longitudinal section through a conidioma E, F conidiogenous cells G conidia H colony on PDA at 14 days I colony on MEA at 14 days. Scale bars: 1 mm ( A ); 200 µm ( B-D ); 10 µm ( E-G ). Description. Conidiomata pycnidial, superficial or immersed, separate, ovoid, 350-500 µm in diam, occasionally aggregated into botryose clusters. Disc black, 200-300 µm in diam. Ostioles single, central, papillate. Conidiophores reduced to conidiogenous cells. Conidiogenous cells hyaline, holoblastic, cylindrical to subcylindrical or broadly lageniform, 7.5-16.0 x 3.5-6.5 μm (av. +/- S.D. = 11.7 +/- 2.2 x 4.6 +/- 0.7 μm ). Conidia1-septate, hazel to blackish brown, mostly truncate at the base and constricted at the septum or with a thickening at the base of the septum, moderately thick-walled, ovoid or oblong to ellipsoidal, 22.5-35.0 x 11.0-19.0 μm (av. +/- S.D. = 27.9 +/- 2.9 x 14.3 +/- 2.2 μm ). Culture characters. Colonies on PDA spreading, covering a 90 mm plate after 14 days at 25 °C, upper white to pale grey, reverse buff to dark grey. Colonies on MEA spreading, covering a 90 mm plate after 14 days at 25 °C, uniform with appressed aerial mycelium and crenate edge, upper white to pale grey, reverse honey to dark grey. Materials examined. China , Beijing City , Mentougou District , Qingshui County , Baihuashan Natural Scenic Area , 39°50'18.21"N , 115°34'21.13"E , on dead branches of Juniperus chinensis , 23 August 2022 , Lu Lin & Xinlei Fan ( holotype BJFC CF20230107, ex-holotype culture CFCC 58549); 39°50'18.16"N , 115°34'21.24"E , on dead branches of Juniperus chinensis , 23 August 2022 , Lu Lin & Xinlei Fan ( paratype BJFC CF20230108, ex-paratype culture CFCC 58788) . Notes. The isolates CFCC 58549 and 58788 in this study formed a distinct linage in the phylogenetic trees of each individual gene (ITS, tef1 -α, and tub2 ) and the combined gene dataset (Fig. 2 ). They were isolated from the branches Juniperus chinensis . Dothiorella iberica was also recorded to host genus Juniperus ( Alves et al. 2013 ). However, these two species are not closely related in our phylogenetic analysis.