299 lines
26 KiB
XML
299 lines
26 KiB
XML
<document ID-CLB-Dataset="53478" ID-DOI="http://dx.doi.org/10.3897/mycokeys.98.103484" ID-GBIF-Dataset="a8094cca-92e5-4638-8da0-0d456b9134ac" ID-Pensoft-Pub="1314-4049-98-19" ID-Pensoft-UUID="EF305950E40550DCB9D464D9FC0264E5" ModsDocID="1314-4049-98-19" checkinTime="1685460034105" checkinUser="pensoft" docAuthor="Liu, Feng, Deng, Yu, Wang, Fei-Hu, Jeewon, Rajesh, Zeng, Qian, Xu, Xiu-Lan, Liu, Ying-Gao & Yang, Chun-Lin" docDate="2023" docId="3CA458FDB4305122B235E65F475059EC" docLanguage="en" docName="MycoKeys 98: 19-35" docOrigin="MycoKeys 98" docPubDate="2023-05-29" docSource="http://dx.doi.org/10.3897/mycokeys.98.103484" docTitle="Microcera pseudaulacaspidis Feng Liu & C. L. Yang, sp. nov." docType="treatment" docVersion="2" id="EF305950E40550DCB9D464D9FC0264E5" lastPageNumber="19" masterDocId="EF305950E40550DCB9D464D9FC0264E5" masterDocTitle="Morphological and molecular analyses reveal two new species of Microcera (Nectriaceae, Hypocreales) associated with scale insects on walnut in China" masterLastPageNumber="35" masterPageNumber="19" pageNumber="19" updateTime="1685460640216" updateUser="ExternalLinkService">
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<mods:title id="0287FB11F3D4484093978951D0B6CDA3">Morphological and molecular analyses reveal two new species of Microcera (Nectriaceae, Hypocreales) associated with scale insects on walnut in China</mods:title>
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<mods:namePart id="65A6906D0338A0B8C51D069A8A8FA583">Liu, Feng</mods:namePart>
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<mods:affiliation id="156EBB120FA890A0E8A4ACA44F4C2E1A">College of Forestry, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China</mods:affiliation>
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<mods:namePart id="BB30CAC65F06AD7BFD5B5DAA997AF686">Deng, Yu</mods:namePart>
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<mods:affiliation id="9E554D4FFF1F56DE6868479474180774">College of Forestry, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China</mods:affiliation>
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<mods:namePart id="07FE5D783098A62C4BE762AB9A983148">Wang, Fei-Hu</mods:namePart>
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<mods:affiliation id="4994F96B3CB40CB49E05612198C8D5C4">College of Forestry, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China</mods:affiliation>
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<mods:namePart id="EF87C3F2E15B20B41D71CC6AF3D088D9">Jeewon, Rajesh</mods:namePart>
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<mods:affiliation id="6B3C09A6EE57970ED95E4CADD910A74F">National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River and Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, College of Forestry, Sichuan Agricultural University, Chengdu, Sichuan, China</mods:affiliation>
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<mods:namePart id="932E753FB84425E9A04BC3CAEBC76ED3">Zeng, Qian</mods:namePart>
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<mods:affiliation id="00586F748DAADB35EAC8754293E135C0">College of Forestry, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China</mods:affiliation>
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<mods:namePart id="3A454308AD82BA2BECF84911A4A90663">Xu, Xiu-Lan</mods:namePart>
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<mods:affiliation id="FDE245A529CD287766A38B1BD279CCD0">Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Reduit, Mauritius</mods:affiliation>
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<mods:namePart id="98252D8130EE28F78F9AE58008955993">Liu, Ying-Gao</mods:namePart>
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<mods:affiliation id="52DA8B66B8098E260509B3F914CD6890">College of Forestry, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China</mods:affiliation>
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<mods:namePart id="308CC7163E3CD0F5831FBC20F598A809">Yang, Chun-Lin</mods:namePart>
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<mods:affiliation id="C1389AEA2DEF31040FA79F79B93DAD68">College of Forestry, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China</mods:affiliation>
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<mods:nameIdentifier id="20F05D8B463B1C3E8A26FC13916EC013" type="email">yangcl0121@163.com</mods:nameIdentifier>
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<mods:title id="42B97BE95C9189C5C6C6F9A555977184">MycoKeys</mods:title>
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<mods:date id="AF0D4CDBBC1FA702025A6A942DE0D323">2023</mods:date>
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<mods:number id="31713B4EB6AB641DF89C2B61BFB38ED0">2023-05-29</mods:number>
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<mods:number id="55B33DE9C1CD0EA99596C6C1669D1796">98</mods:number>
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<mods:start id="F0F871133B6BF37A8580F91508E1A7FC">19</mods:start>
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<mods:end id="4EAECFA9B1F5A220C882000EC038A7BF">35</mods:end>
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<mods:url id="ED4B00840D9059AAA045CC45ADE11F78">http://dx.doi.org/10.3897/mycokeys.98.103484</mods:url>
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<mods:classification id="A52E14927280F76E6AB8ED924B5C59A7">journal article</mods:classification>
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<mods:identifier id="955F304C876CA0AFD7BF404C5EB93DE9" type="DOI">http://dx.doi.org/10.3897/mycokeys.98.103484</mods:identifier>
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<mods:identifier id="BA5311640EAD682911181282438506EF" type="Pensoft-Pub">1314-4049-98-19</mods:identifier>
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<treatment id="3CA458FDB4305122B235E65F475059EC" LSID="urn:lsid:plazi:treatment:3CA458FDB4305122B235E65F475059EC" httpUri="http://treatment.plazi.org/id/3CA458FDB4305122B235E65F475059EC" lastPageNumber="19" pageId="0" pageNumber="19">
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<subSubSection id="0A099E49E6B1FE72BE8BD0C785EBDA69" pageId="0" pageNumber="19" type="nomenclature">
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<paragraph id="2AFC814673028956BDE20D7FEFA0730D" pageId="0" pageNumber="19">
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<taxonomicName id="3F898A9889773A021B998ABBDF0DB5AA" LSID="3CA458FD-B430-5122-B235-E65F475059EC" authority="Feng Liu & C. L. Yang, sp. nov." authorityName="Feng Liu & C. L. Yang, sp. nov." class="Sordariomycetes" family="Nectriaceae" genus="Microcera" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Microcera pseudaulacaspidis" order="Hypocreales" pageId="0" pageNumber="19" phylum="Ascomycota" rank="species" species="pseudaulacaspidis">Microcera pseudaulacaspidis Feng Liu & C.L. Yang, sp. nov.</taxonomicName>
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</paragraph>
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<subSubSection id="3A7AC65A68DB39C819BE73F856D83599" pageId="0" pageNumber="19" type="description">
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<paragraph id="47F416D7C51705246EBC43AB9D351F4D" pageId="0" pageNumber="19">
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<figureCitation id="ADCD43E6FC9F8CD4E6E6B0326D080C61" captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Microcera pseudaulacaspidis (SICAU 22 - 0161) a, b stromata and sporodochia on host substrate c-e conidiophore with developing macroconidia f germinated conidium g-o Macroconidia o, p colonies on PDA after 30 days. Scale bars: 200 µm (a, b); 20 µm (c-e); 10 µm (f-n)." figureDoi="10.3897/mycokeys.98.103484.figure3" httpUri="https://binary.pensoft.net/fig/855946" pageId="0" pageNumber="19">Fig. 3</figureCitation>
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</paragraph>
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<subSubSection id="F6DEB7163AA508996011748CD7790BB8" pageId="0" pageNumber="19" type="etymology">
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<paragraph id="BE4F0DF33A1441220E613586F39EE730" pageId="0" pageNumber="19">Etymology.</paragraph>
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<paragraph id="A29A4B5FF49328DFE2F8BFA4A42D03F7" pageId="0" pageNumber="19">In reference to the generic name of scale insect from which it was isolated.</paragraph>
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</subSubSection>
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<subSubSection id="D25B052847402FDF804FFFCB40720ADB" pageId="0" pageNumber="19" type="holotype">
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<paragraph id="BC1F3E459C2C300CBAAF8625E7BF9D7B" pageId="0" pageNumber="19">Holotype.</paragraph>
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<paragraph id="E115C2D2341CEFF3827277C2AADDB7C3" pageId="0" pageNumber="19">SICAU 22-0161.</paragraph>
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</subSubSection>
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||
<subSubSection id="546B92917E8BDEF86AD03E639799743D" pageId="0" pageNumber="19" type="host">
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<paragraph id="3537F601EC3C6F11C851090A54D47EEC" pageId="0" pageNumber="19">Host.</paragraph>
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<paragraph id="7D23914A2ED4195FF2321B48A7B57FA7" pageId="0" pageNumber="19">
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<taxonomicName id="A726C88052D2FAB8CF8BD2EB4AE56FC0" baseAuthorityName="Targioni Tozzetti" baseAuthorityYear="1886" class="Insecta" family="Diaspididae" genus="Pseudaulacaspis" kingdom="Animalia" lsidName="Pseudaulacaspis pentagona" order="Hemiptera" pageId="0" pageNumber="19" phylum="Arthropoda" rank="species" species="pentagona">
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<emphasis id="D2502793E63AAC418A57FC69ECE32A3C" italics="true" pageId="0" pageNumber="19">Pseudaulacaspis pentagona</emphasis>
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</taxonomicName>
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(
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<taxonomicName id="2512DA79C5395A9C619D063ADF845B9C" class="Insecta" family="Diaspididae" kingdom="Animalia" lsidName="" order="Hemiptera" pageId="0" pageNumber="19" phylum="Arthropoda" rank="family">Diaspididae</taxonomicName>
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,
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<taxonomicName id="D914D6AD6C31F2E2A80A555A5267A183" authorityName="Macgillivray" authorityYear="1921" class="Insecta" kingdom="Animalia" lsidName="" order="Hemiptera" pageId="0" pageNumber="19" phylum="Arthropoda" rank="subOrder" subOrder="Homoptera">Homoptera</taxonomicName>
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)
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</paragraph>
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</subSubSection>
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||
<subSubSection id="59468F0477457C4EDD15FF13A3363C58" pageId="0" pageNumber="19" type="habitat">
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<paragraph id="481C4681867BCE874E0B80AA002BE097" pageId="0" pageNumber="19">Habitat.</paragraph>
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<paragraph id="0617B7742EC2478D1BD792C4BB4D0F51" pageId="0" pageNumber="19">
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On the trunk of
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<taxonomicName id="D465DDBBC8EE4D39FCA53A5F563571D1" class="Magnoliopsida" family="Juglandaceae" genus="Juglans" kingdom="Plantae" lsidName="Juglans regia" order="Fagales" pageId="0" pageNumber="19" phylum="Tracheophyta" rank="species" species="regia">
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<emphasis id="FC3417580B17D79C40341AAF5505886C" italics="true" pageId="0" pageNumber="19">Juglans regia</emphasis>
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</taxonomicName>
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.
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</paragraph>
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</subSubSection>
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<subSubSection id="85AD3577A982319E60FC8DA7CA63A83B" pageId="0" pageNumber="19" type="sexual state">
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<paragraph id="1B25C0F94B2BD6ADCE1C347B50A77CCD" pageId="0" pageNumber="19">Sexual state.</paragraph>
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<paragraph id="1D03D028EE5FBCE4FBE884960E38E789" pageId="0" pageNumber="19">Undetermined.</paragraph>
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</subSubSection>
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<subSubSection id="1092EB5164D5A36A20D2B5C5C18489B0" pageId="0" pageNumber="19" type="asexual state">
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<paragraph id="92A38E77F18EBDE12E18CF7F48B67AEF" pageId="0" pageNumber="19">Asexual state.</paragraph>
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<paragraph id="CC2E3EB11A5C774820FFCF2419BCA4F0" pageId="0" pageNumber="19">
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Stromata byssoid, well-developed, bright orange to orange-red, formed directly on the margin of host scales or their covers with 1-7 sporodochia. Sporodochia 250-900
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<normalizedToken id="71E964E1C746ED2C7596EDEBBFEE3208" originalValue="μm">μm</normalizedToken>
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long, 400-860
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<normalizedToken id="774E0F42385313C25E0D5FB66061F913" originalValue="µm">µm</normalizedToken>
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wide, (x-= 620
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<normalizedToken id="6600FA61D0AAAED97988506472A2DA4A" originalValue="×">x</normalizedToken>
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570
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<normalizedToken id="ED7D12C8028A83C998E0F4DFD902BA34" originalValue="μm">μm</normalizedToken>
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, n = 50), conical, orange-red, upright masses on margin of host scales. Macroconidia 70-120
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<normalizedToken id="E912349C8D4A624E9C407CF96DBD4C61" originalValue="µm">µm</normalizedToken>
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long
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<normalizedToken id="B0EF3E6B38AC7D0ADAFF9D22866961ED" originalValue="×">x</normalizedToken>
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4.2-10.5
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<normalizedToken id="30E1A44FF45031D08F214BB3FA6A8DD5" originalValue="µm">µm</normalizedToken>
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wide (x-= 95.7
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<normalizedToken id="70D6C0BEEFB9492841E539C7C71C022A" originalValue="×">x</normalizedToken>
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6.5
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<normalizedToken id="CFB2BF928DB68596CEE8E2BF5E9A2E46" originalValue="μm">μm</normalizedToken>
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, n = 50), hyaline or jasmine, cylindrical, slightly curved, slender towards each end, 3-10 septate, mostly 7-9 septate, difficult to distinguish apical cell and basal cell. Microconidia and chlamydospores were not observed.
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</paragraph>
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<caption id="A846F6038B15CD0B60410332B6C017AA" doi="10.3897/mycokeys.98.103484.figure3" httpUri="https://binary.pensoft.net/fig/855946" pageId="0" pageNumber="19" start="Figure 3" startId="F3">
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||
<paragraph id="57452012AAD689B63F542D5EC2AAE42B" pageId="0" pageNumber="19">
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<emphasis id="DB82EF6E529D87F2B2C5BAC316D006D0" bold="true" pageId="0" pageNumber="19">Figure 3.</emphasis>
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<taxonomicName id="329B48F8063A3D57A2CAF7D814D4EA67" class="Sordariomycetes" family="Nectriaceae" genus="Microcera" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Microcera pseudaulacaspidis" order="Hypocreales" pageId="0" pageNumber="19" phylum="Ascomycota" rank="species" species="pseudaulacaspidis">
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||
<emphasis id="DBCCBF3E4E4A5FCB8B5E0AE195D2293C" italics="true" pageId="0" pageNumber="19">Microcera pseudaulacaspidis</emphasis>
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</taxonomicName>
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(SICAU 22-0161)
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<emphasis id="909D9128932C84F53FAABD69ED953584" bold="true" pageId="0" pageNumber="19">a, b</emphasis>
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stromata and sporodochia on host substrate
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<emphasis id="B402EEBDD7C12C766A67501E9A709526" bold="true" pageId="0" pageNumber="19">c-e</emphasis>
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conidiophore with developing macroconidia
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<emphasis id="31D7C303C016D116A4A01456C6667C43" bold="true" pageId="0" pageNumber="19">f</emphasis>
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germinated conidium
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||
<emphasis id="EE6C4D49F1AA891EA3E858486E62EA64" bold="true" pageId="0" pageNumber="19">g-o</emphasis>
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Macroconidia
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<emphasis id="C14719F46BA5865F337EC512AE1F1CE8" bold="true" pageId="0" pageNumber="19">o, p</emphasis>
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colonies on PDA after 30 days. Scale bars: 200
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<normalizedToken id="0D17470CEC261486F3AB88EBA830B392" originalValue="µm">µm</normalizedToken>
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(
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<emphasis id="F9155E779EFA999BCAD2164D859B8B08" bold="true" pageId="0" pageNumber="19">a, b</emphasis>
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); 20
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<normalizedToken id="6315F1E2175F13F589A018ECBBF1BB91" originalValue="µm">µm</normalizedToken>
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(
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<emphasis id="FEACBDC84CDF47A6A0BE56D26F539F03" bold="true" pageId="0" pageNumber="19">c-e</emphasis>
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); 10
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<normalizedToken id="5CFC449FE6B4E93778F8B8D76D08971A" originalValue="µm">µm</normalizedToken>
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(
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<emphasis id="7A659D9DCF16DA8D5FB3C5AAE0EAA281" bold="true" pageId="0" pageNumber="19">f-n</emphasis>
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).
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</paragraph>
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</caption>
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</subSubSection>
|
||
<subSubSection id="D32A33E89BCC0A5317793938A2205952" pageId="0" pageNumber="19" type="materials_examined">
|
||
<paragraph id="9AFA1B7B8D0DF23B4951B227C0323317" pageId="0" pageNumber="19">Material examined.</paragraph>
|
||
<paragraph id="80CC8DBFB210089CF38BD2ABC615D17D" pageId="0" pageNumber="19">
|
||
<materialsCitation id="09E1F522FD49ED36B52E44A9BE61908F" collectingDate="2022-04-16" collectorName="Feng Liu, LF" country="China" county="Neijiang City" elevation="340" latitude="29.804167" location="Paifang Village" longLatPrecision="20" longitude="105.11222" municipality="Dongxing District" specimenCount="1" stateProvince="Sichuan Province" typeStatus="holotype">
|
||
<collectingCountry id="6E2B00864C33E2A867C017C0D224972A" name="China">China</collectingCountry>
|
||
,
|
||
<collectingRegion id="42D4CE777EC8767B31800E3B4D722EDF" country="China" name="Sichuan">Sichuan Province</collectingRegion>
|
||
,
|
||
<collectingCounty id="14BBFECFA34F5D7BEA3E0CAA2104900B">Neijiang City</collectingCounty>
|
||
,
|
||
<collectingMunicipality id="13A15694C5B64619FA7A504A6BB1461E">Dongxing District</collectingMunicipality>
|
||
,
|
||
<location id="FBE4A62DAD59F28A2EA04BD7EF914D10" LSID="urn:lsid:plazi:treatment:3CA458FDB4305122B235E65F475059EC:FBE4A62DAD59F28A2EA04BD7EF914D10" country="China" county="Neijiang City" latitude="29.804167" longLatPrecision="20" longitude="105.11222" municipality="Dongxing District" name="Paifang Village" stateProvince="Sichuan Province">Paifang Village</location>
|
||
walnut industrial base (
|
||
<geoCoordinate id="F8C3382F407021113389583659BADA05" degrees="29" direction="north" minutes="48" orientation="latitude" precision="15" seconds="15" value="29.804167">29°48′15″N</geoCoordinate>
|
||
,
|
||
<geoCoordinate id="6B6737BB959681066B8477BAB012E324" degrees="105" direction="east" minutes="06" orientation="longitude" precision="15" seconds="44" value="105.11222">105°06′44″E</geoCoordinate>
|
||
, alt.
|
||
<quantity id="A2A92371A1CBFDACD14AF2A2DBBF7DC7" metricMagnitude="2" metricUnit="m" metricValue="3.4" unit="m" value="340.0">
|
||
<elevation id="49B476C6F6E930D2F2A0457319C80BCB" metricMagnitude="2" metricUnit="m" metricValue="3.4" unit="m" value="340.0">340 m</elevation>
|
||
</quantity>
|
||
), on scale insect
|
||
<taxonomicName id="7257B96756FBA0DA8CD843C5603B4E88" baseAuthorityName="Targioni Tozzetti" baseAuthorityYear="1886" class="Insecta" family="Diaspididae" genus="Pseudaulacaspis" kingdom="Animalia" lsidName="Pseudaulacaspis pentagona" order="Hemiptera" pageId="0" pageNumber="19" phylum="Arthropoda" rank="species" species="pentagona">
|
||
<emphasis id="FA0F38D73D270EA8A00C17A7E3FCCFEE" italics="true" pageId="0" pageNumber="19">Pseudaulacaspis pentagona</emphasis>
|
||
</taxonomicName>
|
||
,
|
||
<collectingDate id="40B3F25931FB686516252D12233B0C74" value="2022-04-16">16 April 2022</collectingDate>
|
||
,
|
||
<collectorName id="AA9FDA898E34A9EF778CEFCFC28F4404">Feng Liu, LF</collectorName>
|
||
202204001, (SICAU 22-0161,
|
||
<typeStatus id="E3CC67A31E580E763DE7C83E2BFB2AFE">holotype</typeStatus>
|
||
), ex-type culture SICAUCC 22-0163
|
||
</materialsCitation>
|
||
.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="38DC39EDFBB1AE9D9F4EEF06A8ECDAA1" pageId="0" pageNumber="19" type="description">
|
||
<paragraph id="3C44832850E3F420201F713AF53FF773" pageId="0" pageNumber="19">Culture characters.</paragraph>
|
||
<paragraph id="0D8AC4377C466BE69603A3D2024E951D" pageId="0" pageNumber="19">
|
||
Colonies from a single macroconidium on PDA grow slowly and reach approximately 2 cm in diameter after 12 days at 25 °C, circular, flat, producing masses of macroconidia in the centre of the colony, measuring 76-125
|
||
<normalizedToken id="CCB607DFEDE096622ADABB1FC89BD416" originalValue="µm">µm</normalizedToken>
|
||
long
|
||
<normalizedToken id="3C65D838E55E9551C1C2C8D2C97FA428" originalValue="×">x</normalizedToken>
|
||
5.3-7.6
|
||
<normalizedToken id="7E314BC569E00044A72C59BDA2FF8401" originalValue="µm">µm</normalizedToken>
|
||
wide (x-= 91.2
|
||
<normalizedToken id="887B44C0F976F9850DCAFC652F6F9056" originalValue="×">x</normalizedToken>
|
||
6.3
|
||
<normalizedToken id="A0E7E78FA32454A0DFEC830B415FDAB8" originalValue="µm">µm</normalizedToken>
|
||
, n = 50), smaller than those in nature, white mycelium on the surface and the back of colonies is dark orange.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="A068962EB41ED7E4927AE1C6E0451FB3" pageId="0" pageNumber="19" type="notes">
|
||
<paragraph id="A348E476FBDEDDB06FCB064145249689" pageId="0" pageNumber="19">Notes.</paragraph>
|
||
<paragraph id="A03C6F0A52449AF3A4AE51422EB48489" pageId="0" pageNumber="19">
|
||
Based on multi-gene phylogenetic analyses,
|
||
<taxonomicName id="59D2CF17A1D961223B095A8005FD8B54" class="Sordariomycetes" family="Nectriaceae" genus="Microcera" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Microcera pseudaulacaspidis" order="Hypocreales" pageId="0" pageNumber="19" phylum="Ascomycota" rank="species" species="pseudaulacaspidis">
|
||
<emphasis id="6E3E5FC40BEF072EB7BD8613FD730B5B" italics="true" pageId="0" pageNumber="19">Microcera pseudaulacaspidis</emphasis>
|
||
</taxonomicName>
|
||
is closely related to
|
||
<taxonomicName id="942FF8B04512C964364A7452BCA47708" lsidName="M. kuwanaspidis" pageId="0" pageNumber="19" rank="species" species="kuwanaspidis">
|
||
<emphasis id="55CFFE2E074DDE766BE8A4696C0D2276" italics="true" pageId="0" pageNumber="19">M. kuwanaspidis</emphasis>
|
||
</taxonomicName>
|
||
(Fig.
|
||
<figureCitation id="E6F21A5A7653E2282421F22C7AE3A2B8" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Phylogram generated from RAxML analysis, based on combined ITS, LSU, tef 1 - α, rpb 1, r pb 2, a cl 1, act, tub 2, cmd A and his 3 sequence data of Microcera isolates. Bootstrap support values from Maximum Likelihood (MLBS, left) higher than 75 % and Bayesian posterior probabilities (BIPP, right) equal to or greater than 0.95 are indicated at the nodes, respectively. The sequences from ex-type strains are in bold. The newly-generated sequence is in red." figureDoi="10.3897/mycokeys.98.103484.figure1" httpUri="https://binary.pensoft.net/fig/855944" pageId="0" pageNumber="19">1</figureCitation>
|
||
). However, we observed significant differences in the DNA sequence data, including base-pair differences and gaps, with values of 1.45% (0 gaps), 17.67% (17 gaps), 3.22% (2 gaps), 1.53% (2 gaps), 1.70% (1 gap) and 3.82% (1 gap) in the ITS, LSU,
|
||
<emphasis id="4287B9A79E65A7F1EED60F928674AB26" italics="true" pageId="0" pageNumber="19">tef</emphasis>
|
||
1-α,
|
||
<emphasis id="290878505BBD1439F065440CD2CF80C4" italics="true" pageId="0" pageNumber="19">tub</emphasis>
|
||
2,
|
||
<emphasis id="C7DA1993562077920C5309E1D0F22F42" italics="true" pageId="0" pageNumber="19">cmd</emphasis>
|
||
A and
|
||
<emphasis id="32F93670E9522A48663B354A3D0921AA" italics="true" pageId="0" pageNumber="19">his</emphasis>
|
||
3 genes, respectively. The PHI test also showed that no significant recombination events between
|
||
<taxonomicName id="8A7CDFE3A26C0B8F8B5A40CB8A33377F" lsidName="M. pseudaulacaspidis" pageId="0" pageNumber="19" rank="species" species="pseudaulacaspidis">
|
||
<emphasis id="6AF0F087B794B4098ABA6EEFC0C34D0F" italics="true" pageId="0" pageNumber="19">M. pseudaulacaspidis</emphasis>
|
||
</taxonomicName>
|
||
and closely phylogenetically-related species occurred (Fig.
|
||
<figureCitation id="1107F2434048CF7FCB04949D0AEDB961" captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. The result of the pairwise homoplasy index (PHI) test of closely-related species using both LogDet transformation and Splits decomposition. PHI test results (Fw) <0.05 indicate significant recombination within the dataset." figureDoi="10.3897/mycokeys.98.103484.figure2" httpUri="https://binary.pensoft.net/fig/855945" pageId="0" pageNumber="19">2</figureCitation>
|
||
). Based on a comparison of their morphological characteristics,
|
||
<taxonomicName id="16BBD8A465E24BEE874B5B198D71015E" lsidName="M. pseudaulacaspidis" pageId="0" pageNumber="19" rank="species" species="pseudaulacaspidis">
|
||
<emphasis id="378B0EFD982D89117BFD3D87B51339D3" italics="true" pageId="0" pageNumber="19">M. pseudaulacaspidis</emphasis>
|
||
</taxonomicName>
|
||
can be distinguished from
|
||
<taxonomicName id="82D62A7432AA0F955100F839814DC7EF" lsidName="M. kuwanaspidis" pageId="0" pageNumber="19" rank="species" species="kuwanaspidis">
|
||
<emphasis id="445EF8470A637DA6CADF667A3041C023" italics="true" pageId="0" pageNumber="19">M. kuwanaspidis</emphasis>
|
||
</taxonomicName>
|
||
by shorter macroconidia (95.7
|
||
<normalizedToken id="F4ED322AF3DF7D1631DBE610D09C5DBE" originalValue="×">x</normalizedToken>
|
||
6.5
|
||
<normalizedToken id="22E061D74A417794B83C8F7C3E54E54D" originalValue="µm">µm</normalizedToken>
|
||
vs. 107
|
||
<normalizedToken id="E2AB2B88E06DA41DF2D7CE7736085CDD" originalValue="×">x</normalizedToken>
|
||
7.3
|
||
<normalizedToken id="F1A14A6789E898A9B44B64F703307B12" originalValue="µm">µm</normalizedToken>
|
||
) with more septa (7-9-septate vs. 5-7-septate) (
|
||
<bibRefCitation id="BDC8580E04F49A65D0C760085074775A" DOI="https://doi.org/10.3390/jof7080628" author="Xu, XL" journalOrPublisher="Journal of Fungi (Basel, Switzerland)" pageId="0" pageNumber="19" pagination=": 628" refId="B46" refString="Xu, XL, Zeng, Q, Lv, YC, Jeewon, R, Maharachchikumbura, S, Wanasinghe, DN, Hyde, KD, Xiao, QG, Liu, YG, Yang, CL, 2021. Insight into the systematics of novel entomopathogenic fungi associated with armored scale insect, Kuwanaspis howardi (Hemiptera: Diaspididae) in China. Journal of Fungi (Basel, Switzerland) 7 (8): 628, DOI: https://doi.org/10.3390/jof7080628" title="Insight into the systematics of novel entomopathogenic fungi associated with armored scale insect, Kuwanaspis howardi (Hemiptera: Diaspididae) in China." url="https://doi.org/10.3390/jof7080628" volume="7" year="2021">Xu et al. 2021</bibRefCitation>
|
||
). Given the morphological dissimilarities, distinct nucleotides at various sites and the well-supported lineage in our phylogeny, we have sufficient evidence to establish
|
||
<taxonomicName id="A0F593A6707A997A7F51EFF000B3F1A3" lsidName="M. pseudaulacaspidis" pageId="0" pageNumber="19" rank="species" species="pseudaulacaspidis">
|
||
<emphasis id="459B55C61165688669CE0320FD6E05B4" italics="true" pageId="0" pageNumber="19">M. pseudaulacaspidis</emphasis>
|
||
</taxonomicName>
|
||
as a new species.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |