treatments-xml/data/03/E2/5F/03E25F447318986C0427F9308205B76D.xml
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<document id="DEEB6DE19EE6E22268D316799BED95F5" ID-DOI="10.1016/j.ijppaw.2015.11.004" ID-ISSN="2213-2244" ID-PMC="PMC4840266" ID-PubMed="27141437" ID-Zenodo-Dep="10668344" IM.bibliography_approvedBy="valdenar" IM.illustrations_approvedBy="valdenar" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_approvedBy="valdenar" IM.taxonomicNames_approvedBy="valdenar" IM.treatments_approvedBy="valdenar" checkinTime="1708029322489" checkinUser="felipe" docAuthor="Mvumbi, Dieudonne ́ Makaba, Bobanga, Thierry Lengu, Umesumbu, Solange Efundu, Kunyu, Billy Shako, Kayembe, Jean-Marie Ntumba, Situakibanza, Nani-Tuma H., Mvumbi, Georges Lelo, Melin, Pierrette, Mol, Patrick De &amp; Hayett, Marie-Pierre" docDate="2016" docId="03E25F447318986C0427F9308205B76D" docLanguage="en" docName="IntJParasitolParasitesWildlife.5.1.1-4.pdf" docOrigin="International Journal for Parasitology: Parasites and Wildlife 5 (1)" docSource="http://dx.doi.org/10.1016/j.ijppaw.2015.11.004" docStyle="DocumentStyle:77CC2CF2FBA7BCD129DD16D0D4F96931.1:IntJParasitolParasitesWildlife.2012-.journal_article" docStyleId="77CC2CF2FBA7BCD129DD16D0D4F96931" docStyleName="IntJParasitolParasitesWildlife.2012-.journal_article" docStyleVersion="1" docTitle="Chromista" docType="treatment" docVersion="4" lastPageNumber="2" masterDocId="FFDB273C7319986D047FFFFC875CB677" masterDocTitle="Investigation on possible transmission of monkeys' Plasmodium to human in a populations living in the equatorial rainforest of the Democratic republic of Congo" masterLastPageNumber="4" masterPageNumber="1" pageNumber="2" updateTime="1711651329193" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-NC-ND-4.0">
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<mods:title id="9F90B19D0B46A8D6F462E4A78838A5CC">Investigation on possible transmission of monkeys' Plasmodium to human in a populations living in the equatorial rainforest of the Democratic republic of Congo</mods:title>
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<mods:namePart id="87229702A6790C63ADE95E444443942A">Mvumbi, Dieudonne ́ Makaba</mods:namePart>
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2.3.
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identi
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cation
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Two real-time PCR were identified in the literature and were successively applied to the DNA templates. The first one allows the detection of all
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spp.
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and the second one allows the detection of the four common
<taxonomicName id="4C4B95D17318986C05D1F8A48525B11B" box="[430,633,1880,1900]" pageId="1" pageNumber="2">
<emphasis id="B93F32407318986C05D1F8A48579B11B" box="[430,549,1880,1900]" italics="true" pageId="1" pageNumber="2">Plasmodium</emphasis>
species
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that infect humans in Africa (
<taxonomicName id="4C4B95D17318986C0571F88886D6B1FF" authorityName="Welch" authorityYear="1897" box="[270,394,1908,1928]" family="Plasmodiidae" genus="Plasmodium" kingdom="Chromista" order="Eucoccidiida" pageId="1" pageNumber="2" phylum="Miozoa" rank="species" species="falciparum">
<emphasis id="B93F32407318986C0571F88886D6B1FF" box="[270,394,1908,1928]" italics="true" pageId="1" pageNumber="2">P. falciparum</emphasis>
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,
<taxonomicName id="4C4B95D17318986C05EBF88886A2B1FF" box="[404,510,1908,1928]" family="Plasmodiidae" genus="Plasmodium" kingdom="Chromista" order="Eucoccidiida" pageId="1" pageNumber="2" phylum="Miozoa" rank="species" species="malariae">
<emphasis id="B93F32407318986C05EBF88886A2B1FF" box="[404,510,1908,1928]" italics="true" pageId="1" pageNumber="2">P. malariae</emphasis>
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,
<taxonomicName id="4C4B95D17318986C0677F8888513B1FF" box="[520,591,1908,1928]" family="Plasmodiidae" genus="Plasmodium" kingdom="Chromista" order="Eucoccidiida" pageId="1" pageNumber="2" phylum="Miozoa" rank="species" species="vivax">
<emphasis id="B93F32407318986C0677F8888513B1FF" box="[520,591,1908,1928]" italics="true" pageId="1" pageNumber="2">P. vivax</emphasis>
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and
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<emphasis id="B93F32407318986C0601F88887D6B1D4" italics="true" pageId="1" pageNumber="2">Plasmodium ovale</emphasis>
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).
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As the species specific Real-Time PCR was more sensitive than the pan primer one, we hypothesized that if the first Real-Time PCR was positive and the second negative, it would suggest that a
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<emphasis id="B93F32407318986C075AFF1684C0B689" box="[805,924,234,254]" italics="true" pageId="1" pageNumber="2">Plasmodium</emphasis>
species
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different from the four human species was detected. In that case, sequencing would be considered.
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