Investigation on possible transmission of monkeys' Plasmodium to human in a populations living in the equatorial rainforest of the Democratic republic of Congo Author Mvumbi, Dieudonne ́ Makaba Biochemistry and Molecular Biology Unit, Department of Basic Sciences, School of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of Author Bobanga, Thierry Lengu Author Umesumbu, Solange Efundu Author Kunyu, Billy Shako Author Kayembe, Jean-Marie Ntumba Author Situakibanza, Nani-Tuma H. Author Mvumbi, Georges Lelo Author Melin, Pierrette Author Mol, Patrick De Author Hayett, Marie-Pierre text International Journal for Parasitology: Parasites and Wildlife 2016 2016-04-30 5 1 1 4 http://dx.doi.org/10.1016/j.ijppaw.2015.11.004 journal article 10.1016/j.ijppaw.2015.11.004 2213-2244 PMC4840266 27141437 10668344 2.3. Plasmodium species identi fi cation Two real-time PCR were identified in the literature and were successively applied to the DNA templates. The first one allows the detection of all Plasmodium spp. and the second one allows the detection of the four common Plasmodium species that infect humans in Africa ( P. falciparum , P. malariae , P. vivax and Plasmodium ovale ). As the species specific Real-Time PCR was more sensitive than the pan primer one, we hypothesized that if the first Real-Time PCR was positive and the second negative, it would suggest that a Plasmodium species different from the four human species was detected. In that case, sequencing would be considered.