242 lines
26 KiB
XML
242 lines
26 KiB
XML
<document id="BFB9A1A94BC641DB9BA7CB98D56AA3EC" ID-CLB-Dataset="27131" ID-DOI="10.11646/zootaxa.4586.1.2" ID-GBIF-Dataset="6c27fa6f-8be8-4b9a-8f3f-f6581007b008" ID-ISSN="1175-5326" ID-Zenodo-Dep="2642667" ID-ZooBank="2D01FAB0-53DF-4B89-A891-A51C548D4B72" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1555445116910" checkinUser="plazi" docAuthor="Morek, Witold, Suzuki, Atsushi C., Schill, Ralph O., Georgiev, Dilian, Yankova, Maria, Marley, Nigel J. & Michalczyk, Łukasz" docDate="2019" docId="922D878EDA473A78FF14FB4DFAFF161F" docLanguage="en" docName="zootaxa.4586.1.2.pdf" docOrigin="Zootaxa 4586 (1)" docStyle="DocumentStyle:647186512141C8FC8976D5BCC54AEB7D.9:Zootaxa.2013-.journal_article" docStyleId="647186512141C8FC8976D5BCC54AEB7D" docStyleName="Zootaxa.2013-.journal_article" docStyleVersion="9" docTitle="Milnesium alpigenum Morek, Suzuki, Schill, Georgiev, Yankova, Marley & Michalczyk, 2019, sp. nov." docType="treatment" docVersion="9" lastPageNumber="55" masterDocId="6E14FFF6DA543A6CFF83FF9DFFF81234" masterDocTitle="Redescription of Milnesium alpigenum Ehrenberg, 1853 (Tardigrada: Apochela) and a description of Milnesium inceptum sp. nov., a tardigrade laboratory model" masterLastPageNumber="64" masterPageNumber="35" pageNumber="54" updateTime="1698721921257" updateUser="ExternalLinkService">
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<mods:title id="43AF5CEF1492BC84B807AFEA625A3EE6">Redescription of Milnesium alpigenum Ehrenberg, 1853 (Tardigrada: Apochela) and a description of Milnesium inceptum sp. nov., a tardigrade laboratory model</mods:title>
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<mods:namePart id="252D06007C1938072F8E1B2EDB801461">Morek, Witold</mods:namePart>
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<mods:namePart id="BD358D6ADF30E4EB9DD6CE5BF5EF75B1">Suzuki, Atsushi C.</mods:namePart>
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<mods:roleTerm id="5AA6C27C67C65ECCF22099208D3F9B1D">Author</mods:roleTerm>
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<mods:namePart id="6956CFFD07049A46268325E48E162413">Schill, Ralph O.</mods:namePart>
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<mods:name id="BC8255C84C5DD2A54EC317CC6CF373BC" type="personal">
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<mods:roleTerm id="36EDC7303E7E44D6030F67747D8FA6B5">Author</mods:roleTerm>
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</mods:role>
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<mods:namePart id="FB90A36551324B40AD63579E4B7A5266">Georgiev, Dilian</mods:namePart>
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</mods:name>
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</mods:role>
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<mods:namePart id="3BB82C4AE83EAF0AC471C8FFA6D89A44">Yankova, Maria</mods:namePart>
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</mods:role>
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<mods:namePart id="65E8ED70C113C9CF66E3AE640535E20E">Marley, Nigel J.</mods:namePart>
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</mods:role>
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<mods:namePart id="25E70FE0DD78D6A40134734F6802B401">Michalczyk, Łukasz</mods:namePart>
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<mods:title id="8F916402538C127A462B6BAE322C864A">Zootaxa</mods:title>
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<mods:part id="C477655DD83C18537849AF3D1FF7C938">
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<mods:date id="A2B731EF6509D0D86F79BC967BE7FE68">2019</mods:date>
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<mods:number id="94449BC59A40E81550E84E1682407411">2019-04-16</mods:number>
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<mods:number id="11E86881592E83712983E81661B22AF9">4586</mods:number>
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<mods:number id="75493DBD9AD2FCF693EEF70CA17DE794">1</mods:number>
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<mods:classification id="B33CD14807449F56152AF54107F1B532">journal article</mods:classification>
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<mods:identifier id="A569B3D15CA6947B30FB33AAFF5B37CB" type="CLB-Dataset">27131</mods:identifier>
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<mods:identifier id="A47FC6B1C1618926E07BE7D00F203525" type="DOI">10.11646/zootaxa.4586.1.2</mods:identifier>
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<mods:identifier id="FA79C3AE75D9150E76FF5EDA7595277F" type="ISSN">1175-5326</mods:identifier>
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</mods:mods>
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<treatment id="922D878EDA473A78FF14FB4DFAFF161F" ID-GBIF-Taxon="156202643" LSID="urn:lsid:plazi:treatment:922D878EDA473A78FF14FB4DFAFF161F" httpUri="http://treatment.plazi.org/id/922D878EDA473A78FF14FB4DFAFF161F" lastPageId="20" lastPageNumber="55" pageId="19" pageNumber="54">
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<subSubSection id="529E6513DA473A7FFF14FB4DFCCA16DE" box="[151,818,1231,1258]" pageId="19" pageNumber="54" type="nomenclature">
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<paragraph id="1A3B3698DA473A7FFF14FB4DFCCA16DE" blockId="19.[151,818,1231,1258]" box="[151,818,1231,1258]" pageId="19" pageNumber="54">
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<heading id="417381F4DA473A7FFF14FB4DFCCA16DE" bold="true" box="[151,818,1231,1258]" fontSize="11" level="1" pageId="19" pageNumber="54" reason="1">
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<emphasis id="28F0EA8ADA473A7FFF14FB4DFCCA16DE" bold="true" box="[151,818,1231,1258]" pageId="19" pageNumber="54">
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Delineation of
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<taxonomicName id="DD844D1BDA473A7FFECEFB52FE0F16DD" authorityName="Ehrenberg" authorityYear="1853" box="[333,503,1231,1257]" class="Polychaeta" family="Aphroditidae" genus="Milnesium" kingdom="Animalia" order="Phyllodocida" pageId="19" pageNumber="54" phylum="Annelida" rank="species" species="alpigenum">
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<emphasis id="28F0EA8ADA473A7FFECEFB52FE0F16DD" bold="true" box="[333,503,1231,1257]" italics="true" pageId="19" pageNumber="54">M. alpigenum</emphasis>
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</taxonomicName>
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and
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<taxonomicName id="DD844D1BDA473A7FFDB7FB52FD3516DD" authority="Morek & Suzuki & Schill & Georgiev & Yankova & Marley & Michalczyk, 2019" authorityName="Morek & Suzuki & Schill & Georgiev & Yankova & Marley & Michalczyk" authorityYear="2019" box="[564,717,1231,1257]" class="Polychaeta" family="Aphroditidae" genus="Milnesium" kingdom="Animalia" order="Phyllodocida" pageId="19" pageNumber="54" phylum="Annelida" rank="species" species="inceptum" status="sp. nov.">
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<emphasis id="28F0EA8ADA473A7FFDB7FB52FD3516DD" bold="true" box="[564,717,1231,1257]" italics="true" pageId="19" pageNumber="54">M. inceptum</emphasis>
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</taxonomicName>
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<taxonomicNameLabel id="33C357F1DA473A7FFD57FB4DFCCA16DE" box="[724,818,1232,1258]" pageId="19" pageNumber="54" rank="species">sp. nov.</taxonomicNameLabel>
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</emphasis>
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</heading>
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||
</paragraph>
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||
</subSubSection>
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||
<subSubSection id="529E6513DA473A78FF14FA8BFAFF161F" lastPageId="20" lastPageNumber="55" pageId="19" pageNumber="54" type="description">
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<paragraph id="1A3B3698DA473A7FFF14FA8BFDC2178A" blockId="19.[151,1437,1302,1867]" pageId="19" pageNumber="54">
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The two species are genetically distinct but morphologically very similar, although not identical. Therefore, they could be classified as pseudocryptic species,
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<emphasis id="28F0EA8ADA473A7FFD12FAA1FD4C1767" box="[657,692,1340,1363]" italics="true" pageId="19" pageNumber="54">i.e.</emphasis>
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species that can be differentiated morphologically but only with a detailed analysis; in this case—with the use of statistical testing of morphometric traits measured in a number of specimens, since the classical identification based on qualitative traits and non-overlapping morphometric ranges is not sufficient to tell the species apart.
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</paragraph>
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<paragraph id="1A3B3698DA473A7FFF44FA57FDC8157F" blockId="19.[151,1437,1302,1867]" pageId="19" pageNumber="54">
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The PCA analysis indicated three components comprising 59.3% of the total variance in the
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<emphasis id="28F0EA8ADA473A7FFB49FA51FB1817D7" box="[1226,1248,1484,1507]" italics="true" pageId="19" pageNumber="54">pt</emphasis>
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ratios. The three factors were as follows: PC1: the
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<emphasis id="28F0EA8ADA473A7FFD9FFA72FDCA1432" box="[540,562,1519,1542]" italics="true" pageId="19" pageNumber="54">pt</emphasis>
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of the primary and secondary branches of all claws (38.2%), PC2: the
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<emphasis id="28F0EA8ADA473A7FFAE5FA72FA841432" box="[1382,1404,1519,1542]" italics="true" pageId="19" pageNumber="54">pt</emphasis>
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of external and posterior spurs (11.3%), and PC3: the
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<emphasis id="28F0EA8ADA473A7FFD70F989FCF1141F" box="[755,777,1556,1579]" italics="true" pageId="19" pageNumber="54">pt</emphasis>
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of buccal tube widths and stylet support insertion point (9.8%). The relationships between the principal components are shown in
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<figureCitation id="82BF2A1DDA473A7FFC5EF9ABFBD8147B" box="[989,1056,1590,1615]" captionStart="FIGURE 3" captionStartId="20.[151,250,1117,1139]" captionTargetBox="[1070,1401,571,1023]" captionTargetId="graphics@20.[1069,1401,599,1023]" captionTargetPageId="20" captionText="FIGURE 3. Graphs illustrating the relationships between the first three principal components revealed by the PCA for the single population of M. alpigenum Ehrenberg, 1853 and the three pooled populations of M. inceptum sp. nov." httpUri="https://zenodo.org/record/2642673/files/figure.png" pageId="19" pageNumber="54">Fig. 3</figureCitation>
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. The two species did not differ in PC1 and PC3 but in contrast, they differed in PC2, thus comparisons of the first three principal components did not result in congruent conclusions. Specifically, when PC1 and PC3 were compared (
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<figureCitation id="82BF2A1DDA473A7FFBD6F9E3FB5714A3" box="[1109,1199,1662,1687]" captionStart="FIGURE 3" captionStartId="20.[151,250,1117,1139]" captionTargetBox="[1070,1401,571,1023]" captionTargetId="graphics@20.[1069,1401,599,1023]" captionTargetPageId="20" captionText="FIGURE 3. Graphs illustrating the relationships between the first three principal components revealed by the PCA for the single population of M. alpigenum Ehrenberg, 1853 and the three pooled populations of M. inceptum sp. nov." httpUri="https://zenodo.org/record/2642673/files/figure.png" pageId="19" pageNumber="54">Fig. 3B</figureCitation>
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), ranges for the two species largely overlapped. In the PC1
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<emphasis id="28F0EA8ADA473A7FFDDDF939FD8D148F" box="[606,629,1700,1723]" italics="true" pageId="19" pageNumber="54">vs</emphasis>
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PC2 comparison (
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<figureCitation id="82BF2A1DDA473A7FFCCDF93FFC53148F" box="[846,939,1698,1723]" captionStart="FIGURE 3" captionStartId="20.[151,250,1117,1139]" captionTargetBox="[1070,1401,571,1023]" captionTargetId="graphics@20.[1069,1401,599,1023]" captionTargetPageId="20" captionText="FIGURE 3. Graphs illustrating the relationships between the first three principal components revealed by the PCA for the single population of M. alpigenum Ehrenberg, 1853 and the three pooled populations of M. inceptum sp. nov." httpUri="https://zenodo.org/record/2642673/files/figure.png" pageId="19" pageNumber="54">Fig. 3A</figureCitation>
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), the overlap between species was smaller. Finally, when PC2 and PC3 were compared, the ranges barely overlapped (
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<figureCitation id="82BF2A1DDA473A7FFC68F95BFBBD14EB" box="[1003,1093,1734,1759]" captionStart="FIGURE 3" captionStartId="20.[151,250,1117,1139]" captionTargetBox="[1070,1401,571,1023]" captionTargetId="graphics@20.[1069,1401,599,1023]" captionTargetPageId="20" captionText="FIGURE 3. Graphs illustrating the relationships between the first three principal components revealed by the PCA for the single population of M. alpigenum Ehrenberg, 1853 and the three pooled populations of M. inceptum sp. nov." httpUri="https://zenodo.org/record/2642673/files/figure.png" pageId="19" pageNumber="54">Fig. 3C</figureCitation>
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). Thus, we compared only the traits constituting PC2 (
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<emphasis id="28F0EA8ADA473A7FFE24F971FE311537" box="[423,457,1772,1795]" italics="true" pageId="19" pageNumber="54">i.e.</emphasis>
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the
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<emphasis id="28F0EA8ADA473A7FFE7FF971FDEA1537" box="[508,530,1772,1795]" italics="true" pageId="19" pageNumber="54">pt</emphasis>
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values of claw spurs) with a series of
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<emphasis id="28F0EA8ADA473A7FFC4DF971FC2E1537" box="[974,982,1772,1795]" italics="true" pageId="19" pageNumber="54">t</emphasis>
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-tests and adjusted α-levels. Student’s
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<emphasis id="28F0EA8ADA473A7FFA08F971FA6B1537" box="[1419,1427,1772,1795]" italics="true" pageId="19" pageNumber="54">t</emphasis>
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- tests revealed significant differences in three of the eight compared traits (mean values, ±
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<collectingRegion id="D840F87ADA473A7FFB0DF892FB4C1513" box="[1166,1204,1807,1831]" country="Serbia" name="Sid" pageId="19" pageNumber="54">SD</collectingRegion>
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, and [ranges] for
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<emphasis id="28F0EA8ADA473A7FFAFEF892FE47157F" italics="true" pageId="19" pageNumber="54">
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<taxonomicName id="DD844D1BDA473A7FFAFEF892FEF5157F" authorityName="Ehrenberg" authorityYear="1853" class="Polychaeta" family="Aphroditidae" genus="Milnesium" kingdom="Animalia" order="Phyllodocida" pageId="19" pageNumber="54" phylum="Annelida" rank="species" species="alpigenum">M. alpigenum</taxonomicName>
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vs
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<taxonomicName id="DD844D1BDA473A7FFEB1F8A9FE47157F" authority="Morek & Suzuki & Schill & Georgiev & Yankova & Marley & Michalczyk, 2019" authorityName="Morek & Suzuki & Schill & Georgiev & Yankova & Marley & Michalczyk" authorityYear="2019" box="[306,447,1844,1867]" class="Polychaeta" family="Aphroditidae" genus="Milnesium" kingdom="Animalia" order="Phyllodocida" pageId="19" pageNumber="54" phylum="Annelida" rank="species" species="inceptum" status="sp. nov.">M. inceptum</taxonomicName>
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</emphasis>
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||
<taxonomicNameLabel id="33C357F1DA473A7FFE46F8AFFDE7157F" box="[453,543,1842,1867]" pageId="19" pageNumber="54" rank="species">
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||
<emphasis id="28F0EA8ADA473A7FFE46F8AFFDE7157F" bold="true" box="[453,543,1842,1867]" pageId="19" pageNumber="54">sp. nov.</emphasis>
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</taxonomicNameLabel>
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):
|
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</paragraph>
|
||
<paragraph id="1A3B3698DA473A7FFF14F8E6FB4F15A7" blockId="19.[151,1230,1914,2021]" box="[151,1207,1914,1943]" pageId="19" pageNumber="54">
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• spur on external claw I:
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<emphasis id="28F0EA8ADA473A7FFE55F8E6FDFF15A7" box="[470,519,1915,1939]" italics="true" pageId="19" pageNumber="54">13.3</emphasis>
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±
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<emphasis id="28F0EA8ADA473A7FFD9EF8E6FDB815A7" box="[541,576,1915,1939]" italics="true" pageId="19" pageNumber="54">1.5</emphasis>
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||
[
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||
<emphasis id="28F0EA8ADA473A7FFDD2F8E6FD4615A7" box="[593,702,1915,1939]" italics="true" pageId="19" pageNumber="54">11.3–17.8</emphasis>
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]
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||
<emphasis id="28F0EA8ADA473A7FFD4CF8E1FCAD15A7" box="[719,853,1915,1939]" italics="true" pageId="19" pageNumber="54">vs 11.3 ±1.3</emphasis>
|
||
[
|
||
<emphasis id="28F0EA8ADA473A7FFCE5F8E7FC3015A7" box="[870,968,1914,1939]" italics="true" pageId="19" pageNumber="54">7.6–14.8</emphasis>
|
||
],
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||
<emphasis id="28F0EA8ADA473A7FFC5CF8E1FC1F15A7" box="[991,999,1916,1939]" italics="true" pageId="19" pageNumber="54">t</emphasis>
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<subScript id="860034DDDA473A7FFC64F814FC0E15A3" attach="left" box="[999,1014,1929,1943]" fontSize="6" pageId="19" pageNumber="54">46</subScript>
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=4.002,
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<emphasis id="28F0EA8ADA473A7FFBD0F8E1FB9915A7" box="[1107,1121,1916,1939]" italics="true" pageId="19" pageNumber="54">p</emphasis>
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<0.001;
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</paragraph>
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<paragraph id="1A3B3698DA473A7FFF14F83FFB3D158E" blockId="19.[151,1230,1914,2021]" box="[151,1221,1953,1982]" pageId="19" pageNumber="54">
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• spur on external claw II:
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<emphasis id="28F0EA8ADA473A7FFE5CF83FFDB1158E" box="[479,585,1954,1978]" italics="true" pageId="19" pageNumber="54">15.3 ±1.4</emphasis>
|
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[
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<emphasis id="28F0EA8ADA473A7FFDD9F83FFD32158E" box="[602,714,1954,1978]" italics="true" pageId="19" pageNumber="54">12.7–17.5</emphasis>
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]
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<emphasis id="28F0EA8ADA473A7FFD59F83FFC9B158E" box="[730,867,1954,1978]" italics="true" pageId="19" pageNumber="54">vs 12.9 ±2.0</emphasis>
|
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[
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<emphasis id="28F0EA8ADA473A7FFCF7F83CFC2D158E" box="[884,981,1953,1978]" italics="true" pageId="19" pageNumber="54">9.2–16.6</emphasis>
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],
|
||
<emphasis id="28F0EA8ADA473A7FFC6EF83FFC0D158D" box="[1005,1013,1954,1977]" italics="true" pageId="19" pageNumber="54">t</emphasis>
|
||
<subScript id="860034DDDA473A7FFC77F82DFBFB158A" attach="left" box="[1012,1027,1968,1982]" fontSize="6" pageId="19" pageNumber="54">54</subScript>
|
||
=4.630,
|
||
<emphasis id="28F0EA8ADA473A7FFBE2F83FFB97158D" box="[1121,1135,1954,1977]" italics="true" pageId="19" pageNumber="54">p</emphasis>
|
||
<0.001;
|
||
</paragraph>
|
||
<paragraph id="1A3B3698DA473A7FFF14F854FB3615D4" blockId="19.[151,1230,1914,2021]" box="[151,1230,1992,2021]" pageId="19" pageNumber="54">
|
||
• spur on external claw III:
|
||
<emphasis id="28F0EA8ADA473A7FFE6AF855FDAB15D4" box="[489,595,1992,2016]" italics="true" pageId="19" pageNumber="54">15.2 ±1.4</emphasis>
|
||
[
|
||
<emphasis id="28F0EA8ADA473A7FFDE7F855FD2C15D4" box="[612,724,1992,2016]" italics="true" pageId="19" pageNumber="54">12.4–17.8</emphasis>
|
||
]
|
||
<emphasis id="28F0EA8ADA473A7FFD67F854FC9515D4" box="[740,877,1992,2016]" italics="true" pageId="19" pageNumber="54">vs 12.4 ±1.8</emphasis>
|
||
[
|
||
<emphasis id="28F0EA8ADA473A7FFCFEF855FC2715D5" box="[893,991,1992,2017]" italics="true" pageId="19" pageNumber="54">8.9–16.8</emphasis>
|
||
],
|
||
<emphasis id="28F0EA8ADA473A7FFC75F854FC0615D4" box="[1014,1022,1993,2016]" italics="true" pageId="19" pageNumber="54">t</emphasis>
|
||
<subScript id="860034DDDA473A7FFC7DF84AFBF515D1" attach="left" box="[1022,1037,2007,2021]" fontSize="6" pageId="19" pageNumber="54">48</subScript>
|
||
=5.758,
|
||
<emphasis id="28F0EA8ADA473A7FFBE9F854FB8015D4" box="[1130,1144,1993,2016]" italics="true" pageId="19" pageNumber="54">p</emphasis>
|
||
<0.001.
|
||
</paragraph>
|
||
<paragraph id="1A3B3698DA403A78FF44FF05FBA012E0" blockId="20.[151,1437,151,501]" pageId="20" pageNumber="55">
|
||
In other words, the analysis showed that
|
||
<taxonomicName id="DD844D1BDA403A78FD22FF04FCC71284" authorityName="Ehrenberg" authorityYear="1853" box="[673,831,151,176]" class="Polychaeta" family="Aphroditidae" genus="Milnesium" kingdom="Animalia" order="Phyllodocida" pageId="20" pageNumber="55" phylum="Annelida" rank="species" species="alpigenum">
|
||
<emphasis id="28F0EA8ADA403A78FD22FF04FCC71284" box="[673,831,151,176]" italics="true" pageId="20" pageNumber="55">M. alpigenum</emphasis>
|
||
</taxonomicName>
|
||
has statistically longer (relatively to the buccal tube length) external spurs than
|
||
<taxonomicName id="DD844D1BDA403A78FE4BFF20FDAC12E0" authority="Morek & Suzuki & Schill & Georgiev & Yankova & Marley & Michalczyk, 2019" authorityName="Morek & Suzuki & Schill & Georgiev & Yankova & Marley & Michalczyk" authorityYear="2019" box="[456,596,189,212]" class="Polychaeta" family="Aphroditidae" genus="Milnesium" kingdom="Animalia" order="Phyllodocida" pageId="20" pageNumber="55" phylum="Annelida" rank="species" species="inceptum" status="sp. nov.">
|
||
<emphasis id="28F0EA8ADA403A78FE4BFF20FDAC12E0" box="[456,596,189,212]" italics="true" pageId="20" pageNumber="55">M. inceptum</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="33C357F1DA403A78FDD8FF21FD4C12E1" box="[603,692,188,213]" pageId="20" pageNumber="55" rank="species">
|
||
<emphasis id="28F0EA8ADA403A78FDD8FF21FD4C12E1" bold="true" box="[603,692,188,213]" pageId="20" pageNumber="55">sp. nov.</emphasis>
|
||
</taxonomicNameLabel>
|
||
(compare also
|
||
<figureCitation id="82BF2A1DDA403A78FCDCFF21FC6412E0" box="[863,924,188,213]" captionStart="FIGURE 1" captionStartId="9.[151,250,1887,1909]" captionTargetBox="[390,1211,189,1846]" captionTargetId="figure@9.[390,1211,189,1846]" captionTargetPageId="9" captionText="FIGURE 1. Milnesium alpigenum Ehrenberg, 1853. A—habitus, ventral view (neotype, PCM). B—habitus, dorsal view (SEM). C—dorsal cuticle, the arrow indicates area with visible pseudopores (neotype, PCM). D—dorsal cuticle with the barely visible outline of a pseudoplate (neoparatype, SEM). E—buccal apparatus (neotype, PCM). F—six peribuccal lamellae with the 4+2 configuration (neoparatype, SEM). G—claws III with the cuticular bar below (neoparatype, PCM). H—claws IV (neotype, PCM). All scale bars in µm." httpUri="https://zenodo.org/record/2642669/files/figure.png" pageId="20" pageNumber="55">Fig. 1</figureCitation>
|
||
G–H and 2H–I).
|
||
</paragraph>
|
||
<paragraph id="1A3B3698DA403A78FF44FF7DFD4213BC" blockId="20.[151,1437,151,501]" pageId="20" pageNumber="55">
|
||
In contrast to subtle morphometric differences, the two species exhibit considerable genetic distances in all four analysed markers. Specifically, they differ by 1.0% in 18S rRNA, 5.2% in 28S rRNA, 21.6% in ITS-2, and by 18.1% in COI. Most importantly, however, the two species are not immediately related to each other (see
|
||
<figureCitation id="82BF2A1DDA403A78FAB7FEBAFA8D1374" box="[1332,1397,295,320]" captionStart="FIGURE 4" captionStartId="21.[151,250,1306,1328]" captionTargetBox="[220,1389,455,1242]" captionTargetId="graphics@21.[268,1009,455,1142]" captionTargetPageId="21" captionText="FIGURE 4. The positions of M. alpigenum Ehrenberg, 1853 and M. inceptum sp. nov. on the Milnesium phylogenetic tree based on the concatenated data set of ITS-2 and COI sequences. Branch support values are BI posterior probabilities. Species names in square brackets and in grey font are GenBank labels that are incorrect species identifications, uncertain identifications, or invalid names (correct identifications are provided in black font, before the incorrect labels). Filled circles represent both ITS-2 and COI sequences, empty circles indicate COI sequences only. Scale bar shows the number of substitutions per site." httpUri="https://zenodo.org/record/2642675/files/figure.png" pageId="20" pageNumber="55">Fig. 4</figureCitation>
|
||
for the positions of both species on the
|
||
<taxonomicName id="DD844D1BDA403A78FDBEFED1FD4B1351" authorityName="Doyere" authorityYear="1840" box="[573,691,332,357]" class="Polychaeta" family="Aphroditidae" genus="Milnesium" kingdom="Animalia" order="Phyllodocida" pageId="20" pageNumber="55" phylum="Annelida" rank="genus">
|
||
<emphasis id="28F0EA8ADA403A78FDBEFED1FD4B1351" box="[573,691,332,357]" italics="true" pageId="20" pageNumber="55">Milnesium</emphasis>
|
||
</taxonomicName>
|
||
phylogenetic tree), which is the strongest evidence that the two species represent separate phylogenetic lineages.
|
||
</paragraph>
|
||
<paragraph id="1A3B3698DA403A78FF44FE08FEA913C1" blockId="20.[151,1437,151,501]" pageId="20" pageNumber="55">
|
||
To conclude, differences both in phenotypic and genetic traits unequivocally show that
|
||
<taxonomicName id="DD844D1BDA403A78FB15FE08FADB1398" authority="Morek & Suzuki & Schill & Georgiev & Yankova & Marley & Michalczyk, 2019" authorityName="Morek & Suzuki & Schill & Georgiev & Yankova & Marley & Michalczyk" authorityYear="2019" box="[1174,1315,405,428]" class="Polychaeta" family="Aphroditidae" genus="Milnesium" kingdom="Animalia" order="Phyllodocida" pageId="20" pageNumber="55" phylum="Annelida" rank="species" species="inceptum" status="sp. nov.">
|
||
<emphasis id="28F0EA8ADA403A78FB15FE08FADB1398" box="[1174,1315,405,428]" italics="true" pageId="20" pageNumber="55">M. inceptum</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="33C357F1DA403A78FAAAFE09FA7B1399" box="[1321,1411,404,429]" pageId="20" pageNumber="55" rank="species">
|
||
<emphasis id="28F0EA8ADA403A78FAAAFE09FA7B1399" bold="true" box="[1321,1411,404,429]" pageId="20" pageNumber="55">sp. nov.</emphasis>
|
||
</taxonomicNameLabel>
|
||
is a
|
||
<emphasis id="28F0EA8ADA403A78FF31FE2AFEB213E4" box="[178,330,439,464]" italics="true" pageId="20" pageNumber="55">bona species</emphasis>
|
||
. Nevertheless, an extreme care must be taken when identifying these species using solely phenotypic data.
|
||
</paragraph>
|
||
<paragraph id="1A3B3698DA403A78FF26FDA6FBD01047" blockId="20.[165,1401,571,1067]" pageId="20" pageNumber="55">
|
||
<emphasis id="28F0EA8ADA403A78FF26FDA6FBF31062" bold="true" box="[165,1035,571,598]" pageId="20" pageNumber="55">A B C</emphasis>
|
||
<subScript id="860034DDDA403A78FDEEFDF4FBD01047" attach="right" box="[621,1064,617,627]" fontSize="4" pageId="20" pageNumber="55">0.2 0.2</subScript>
|
||
</paragraph>
|
||
<paragraph id="1A3B3698DA403A78FF46FD16FF2C10A1" blockId="20.[165,1401,571,1067]" box="[197,212,651,661]" pageId="20" pageNumber="55">0.2</paragraph>
|
||
<paragraph id="1A3B3698DA403A78FDEEFD58FF2C10EB" blockId="20.[165,1401,571,1067]" pageId="20" pageNumber="55">0.1 0.1 0.1</paragraph>
|
||
<paragraph id="1A3B3698DA403A78FF29FD63FBF4117C" blockId="20.[165,1401,571,1067]" pageId="20" pageNumber="55">))) % % % 9 0 0... 2 0 0 1 0.0 1 0.0 1 0.0 (((2 3 3 PC PC PC</paragraph>
|
||
<paragraph id="1A3B3698DA403A78FF42FCF4FBD011B2" blockId="20.[165,1401,571,1067]" box="[193,1064,873,902]" pageId="20" pageNumber="55">-0.1 -0.1 -0.1</paragraph>
|
||
<paragraph id="1A3B3698DA403A78FF42FC2FFF2C1188" blockId="20.[165,1401,571,1067]" box="[193,212,946,956]" pageId="20" pageNumber="55">-0.2</paragraph>
|
||
<paragraph id="1A3B3698DA403A78FDEAFC4AFBD011D6" blockId="20.[165,1401,571,1067]" box="[617,1064,983,994]" pageId="20" pageNumber="55">-0.2 -0.2</paragraph>
|
||
<paragraph id="1A3B3698DA403A78FF7FFC63FAFF161F" blockId="20.[165,1401,571,1067]" pageId="20" pageNumber="55">-0.2 0.0 0.2 -0.2 0.0 0.2 -0.1 0.0 -0.1 PC1 (3 5.8%) PC1 (3 5.8%) PC2 (1 2.9%)</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |