treatments-xml/data/03/95/B0/0395B06BFFCFFFAD7B53C50AFB3DF84F.xml

<document id="4F72E22F6FFC8BA538F5EB5C3AB98D59" ID-CLB-Dataset="300338" ID-DOI="10.22271/fish.2024.v12.i1a.2893" ID-GBIF-Dataset="5e0ea56f-c06a-4f6d-9a21-b389d8206297" ID-ISSN="2347-5129" ID-Zenodo-Dep="12550280" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="juliana" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_approvedBy="juliana" IM.taxonomicNames_approvedBy="juliana" IM.treatments_approvedBy="juliana" checkinTime="1719420516578" checkinUser="felipe" docAuthor="Choudhury, Mrinmoy, Deka, Prantik &amp; Purkayastha, Debashree" docDate="2024" docId="0395B06BFFCFFFAD7B53C50AFB3DF84F" docLanguage="en" docName="IntJFishAquatStud.12.1.A.63-66.pdf" docOrigin="International Journal of Fisheries and Aquatic Studies 12 (1)" docSource="http://dx.doi.org/10.22271/fish.2024.v12.i1a.2893" docStyle="DocumentStyle:4C58C81CAA25A52E947307742031BFD1.3:IntJFishAquatStud.2021-.journal_article" docStyleId="4C58C81CAA25A52E947307742031BFD1" docStyleName="IntJFishAquatStud.2021-.journal_article" docStyleVersion="3" docTitle="Channa punctata" docType="treatment" docVersion="2" lastPageNumber="65" masterDocId="FFACC813FFCEFFAF7B33C171FFEFFFFB" masterDocTitle="Sub-lethal toxic effect of cadmium chloride (CdCl) on freshwater murrel Channa punctata (BLOCH)" masterLastPageNumber="66" masterPageNumber="63" pageNumber="64" updateTime="1722265097404" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:title id="5BE6BE1853DCA1F6ADFC3888967C077F">Sub-lethal toxic effect of cadmium chloride (CdCl) on freshwater murrel Channa punctata (BLOCH)</mods:title>
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<mods:namePart id="21541CD7EB5985DEFA48CD05C3392165">Choudhury, Mrinmoy</mods:namePart>
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<mods:namePart id="941E1A8451BF939EA41343FA4E23C7F4">Deka, Prantik</mods:namePart>
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<mods:namePart id="C7E83C4D02B6307D40911D32F0776302">Purkayastha, Debashree</mods:namePart>
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<mods:title id="177729E23C88A3A33349B128F84E7ADD">International Journal of Fisheries and Aquatic Studies</mods:title>
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<mods:date id="D7EFC043CC5A09473ADDB9602E490C48">2024</mods:date>
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<heading id="D0CBB611FFCFFFAE7B53C50AFECFFB68" box="[96,288,1147,1171]" fontSize="36" level="2" pageId="1" pageNumber="64" reason="5">
<emphasis id="B948DD6FFFCFFFAE7B53C50AFF53FB68" bold="true" box="[96,188,1147,1171]" pageId="1" pageNumber="64">Species:</emphasis>
<taxonomicName id="4C3C7AFEFFCFFFAE7BF0C50DFECFFB68" ID-CoL="TM48" authority="(Bloch)" baseAuthorityName="Bloch" box="[195,288,1148,1171]" family="Channidae" genus="Channa" kingdom="Animalia" order="Perciformes" pageId="1" pageNumber="64" phylum="Chordata" rank="species" species="punctata">
<emphasis id="B948DD6FFFCFFFAE7BF0C50DFECFFB68" box="[195,288,1148,1171]" italics="true" pageId="1" pageNumber="64">punctata</emphasis>
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<paragraph id="8B83017DFFCFFFAE7A0FC4C9FDF1FA36" blockId="1.[316,542,1464,1486]" box="[316,542,1464,1486]" pageId="1" pageNumber="64">
<emphasis id="B948DD6FFFCFFFAE7A0FC4C9FE97FA36" bold="true" box="[316,376,1464,1485]" pageId="1" pageNumber="64">Fig 1:</emphasis>
<taxonomicName id="4C3C7AFEFFCFFFAE7A4DC4C9FDF1FA36" box="[382,542,1464,1486]" family="Channidae" genus="Channa" kingdom="Animalia" order="Perciformes" pageId="1" pageNumber="64" phylum="Chordata" rank="species" species="punctata">Channa punctata</taxonomicName>
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<paragraph id="8B83017DFFCFFFAE7B53C485FE86F9F7" blockId="1.[96,361,1524,1548]" box="[96,361,1524,1548]" pageId="1" pageNumber="64">
<heading id="D0CBB611FFCFFFAE7B53C485FE86F9F7" bold="true" box="[96,361,1524,1548]" fontSize="36" level="1" pageId="1" pageNumber="64" reason="1">
<emphasis id="B948DD6FFFCFFFAE7B53C485FE86F9F7" bold="true" box="[96,361,1524,1548]" pageId="1" pageNumber="64">Materials and Methods</emphasis>
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<heading id="D0CBB611FFCFFFAE7B53C763FDF6F9D1" bold="true" box="[96,537,1554,1578]" fontSize="10" level="2" pageId="1" pageNumber="64" reason="3">
<emphasis id="B948DD6FFFCFFFAE7B53C763FDF6F9D1" bold="true" box="[96,537,1554,1578]" pageId="1" pageNumber="64">Collection and maintenance of test fish</emphasis>
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<taxonomicName id="4C3C7AFEFFCFFFAE7B53C741FECDF9B3" box="[96,290,1584,1608]" family="Channidae" genus="Channa" kingdom="Animalia" order="Perciformes" pageId="1" pageNumber="64" phylum="Chordata" rank="species" species="punctata">
<emphasis id="B948DD6FFFCFFFAE7B53C741FECDF9B3" box="[96,290,1584,1608]" italics="true" pageId="1" pageNumber="64">Channa punctata</emphasis>
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were collected from culture pond near Serispore T.E., Hailakandi, 
<collectingRegion id="49F8CF9FFFCFFFAE7AA0C73EFE31F99C" box="[403,478,1615,1639]" country="India" name="Assam" pageId="1" pageNumber="64">Assam</collectingRegion>
, 
<collectingCountry id="F32B41EDFFCFFFAE7ADFC73EFDCCF99C" box="[492,547,1615,1639]" name="India" pageId="1" pageNumber="64">India</collectingCountry>
and brought to the laboratory in aerated containers. In the laboratory, the fishes were maintained in an aquarium with constant aeration. Fishes were fed with commercially available fish meal twice daily and were acclimatized in the aquarium for a week before the experiment.
</paragraph>
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<emphasis id="B948DD6FFFCFFFAE780BC1F6FB30FF64" bold="true" box="[824,1247,135,159]" pageId="1" pageNumber="64">Experimental design of fish bio-assay</emphasis>
</heading>
</paragraph>
<paragraph id="8B83017DFFCFFFAE780BC1D7FA69FEE2" blockId="1.[824,1491,165,281]" pageId="1" pageNumber="64">
For test setup, 
<taxonomicName id="4C3C7AFEFFCFFFAE78E4C1D4FB60FF46" box="[983,1167,165,189]" family="Channidae" genus="Channa" kingdom="Animalia" order="Perciformes" pageId="1" pageNumber="64" phylum="Chordata" rank="species" species="punctata">
<emphasis id="B948DD6FFFCFFFAE78E4C1D4FB60FF46" box="[983,1167,165,189]" italics="true" pageId="1" pageNumber="64">Channa punctata</emphasis>
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were kept in a tank (Group-I) with heavy metal (CdCl 
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) for seven days. A control with no treatment was also run simultaneously in another tank. After seventh day, the protein content of liver was examined.
</paragraph>
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<emphasis id="B948DD6FFFCFFFAE780BC04EFBC8FEAC" bold="true" box="[824,1063,319,343]" pageId="1" pageNumber="64">Experimental Design</emphasis>
</heading>
</paragraph>
<paragraph id="8B83017DFFCFFFAE780BC02CFAC4FD72" blockId="1.[824,1491,349,649]" pageId="1" pageNumber="64">
The fishes were randomly divided into three groups 
<emphasis id="B948DD6FFFCFFFAE7E9BC02FFA3CFE8E" box="[1448,1491,350,373]" italics="true" pageId="1" pageNumber="64">viz.,</emphasis>
control, low dose, sub-lethal-1 (SL-I, 5ppm) and high dose sub-lethal 2(SL-II, 10ppm). They were kept in three different buckets containing 5 litres of water obtained from a pond and were labelled as control, SL-1(5ppm) and SL-2 (10ppm). The experiment was run for seven days. Fishes were taken out from all the tanks after seven days and were anesthetized and the total length and body weight were recorded. Test fishes were sacrificed and tissues like gills and liver were dissected and stored at refrigerator until further analysis.
</paragraph>
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<heading id="D0CBB611FFCFFFAE780BC3DEFB69FD1D" bold="true" fontSize="10" level="2" pageId="1" pageNumber="64" reason="3">
<emphasis id="B948DD6FFFCFFFAE780BC3DEFB69FD1D" bold="true" pageId="1" pageNumber="64">Procedure for estimation of total protein content Estimation of protein content</emphasis>
</heading>
</paragraph>
<paragraph id="8B83017DFFCFFFAE780BC39DFBDEFCD9" blockId="1.[824,1491,748,803]" pageId="1" pageNumber="64">
Total protein content was estimated by the protocol developed by Lowry 
<emphasis id="B948DD6FFFCFFFAE7894C27DFC32FCD9" box="[935,989,778,803]" italics="true" pageId="1" pageNumber="64">et al.</emphasis>
(1951).
</paragraph>
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<heading id="D0CBB611FFCFFFAE780BC238FC35FC9A" bold="true" box="[824,986,841,865]" fontSize="10" level="2" pageId="1" pageNumber="64" reason="3">
<emphasis id="B948DD6FFFCFFFAE780BC238FC35FC9A" bold="true" box="[824,986,841,865]" pageId="1" pageNumber="64">Reagents used</emphasis>
</heading>
</paragraph>
<paragraph id="8B83017DFFCFFFAE780BC216FB67FC84" blockId="1.[824,1491,871,1232]" box="[824,1160,871,895]" pageId="1" pageNumber="64">
<heading id="D0CBB611FFCFFFAE780BC216FB67FC84" bold="true" box="[824,1160,871,895]" fontSize="10" level="2" pageId="1" pageNumber="64" reason="3">
<emphasis id="B948DD6FFFCFFFAE780BC216FB67FC84" bold="true" box="[824,1160,871,895]" pageId="1" pageNumber="64">A. Alkaline copper Reagent.</emphasis>
</heading>
</paragraph>
<paragraph id="8B83017DFFCFFFAE780BC2F7FB00FC66" blockId="1.[824,1491,871,1232]" box="[824,1263,901,926]" pageId="1" pageNumber="64">
Reagent A: 2 % Na 
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CO 
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in
</quantity>
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NaOH.
</paragraph>
<paragraph id="8B83017DFFCFFFAE780BC2D4FA2DFC47" blockId="1.[824,1491,871,1232]" box="[824,1474,932,957]" pageId="1" pageNumber="64">
Reagent B: 0.5% CuSO 
<subScript id="17B80338FFCFFFAE7F06C2DFFBADFC47" attach="left" box="[1077,1090,932,956]" fontSize="6" pageId="1" pageNumber="64">4.</subScript>
5H 
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O in sodium potassium tartarate
</paragraph>
<paragraph id="8B83017DFFCFFFAE780BC2B2FB7AFC02" blockId="1.[824,1491,871,1232]" pageId="1" pageNumber="64">The alkaline copper reagent was made by mixing 50 ml of reagent A and 1 ml of reagent B.</paragraph>
<paragraph id="8B83017DFFCFFFAE780BC570FC48FBAE" blockId="1.[824,1491,871,1232]" pageId="1" pageNumber="64">B. Folin Ciocalteu phenol reagent (It is commercially available which is diluted with distilled water in the ratio of 1:2</paragraph>
<paragraph id="8B83017DFFCFFFAE780BC52DFC06FB8F" blockId="1.[824,1491,871,1232]" box="[824,1001,1116,1140]" pageId="1" pageNumber="64">
C. 
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NaOH
</paragraph>
<paragraph id="8B83017DFFCFFFAE780BC50AFC01FB68" blockId="1.[824,1491,871,1232]" box="[824,1006,1147,1171]" pageId="1" pageNumber="64">D. 70% alcohol</paragraph>
<paragraph id="8B83017DFFCFFFAE780BC5EBFB2BFB2B" blockId="1.[824,1491,871,1232]" pageId="1" pageNumber="64">
E. Protein (stock) Standard solution: 
<quantity id="4CC4AC98FFCFFFAE7E31C5E8FAA3FB4A" box="[1282,1356,1177,1201]" metricMagnitude="-4" metricUnit="kg" metricValue="1.0" pageId="1" pageNumber="64" unit="mg" value="100.0">100mg</quantity>
% Bovine Serum Albumin in 
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NaOH.
</paragraph>
<paragraph id="8B83017DFFCFFFAE780BC587FBE4FAF5" blockId="1.[824,1035,1270,1294]" box="[824,1035,1270,1294]" pageId="1" pageNumber="64">
<heading id="D0CBB611FFCFFFAE780BC587FBE4FAF5" bold="true" box="[824,1035,1270,1294]" fontSize="10" level="2" pageId="1" pageNumber="64" reason="3">
<emphasis id="B948DD6FFFCFFFAE780BC587FBE4FAF5" bold="true" box="[824,1035,1270,1294]" pageId="1" pageNumber="64">Working standard</emphasis>
</heading>
</paragraph>
<paragraph id="8B83017DFFCFFFAE780BC465FA53FAD7" blockId="1.[824,1468,1300,1324]" box="[824,1468,1300,1324]" pageId="1" pageNumber="64">10 ml of the stock was diluted to 100ml with distilled water.</paragraph>
<paragraph id="8B83017DFFCFFFAE780BC423FC25FA91" blockId="1.[824,970,1362,1386]" box="[824,970,1362,1386]" pageId="1" pageNumber="64">
<heading id="D0CBB611FFCFFFAE780BC423FC25FA91" bold="true" box="[824,970,1362,1386]" fontSize="10" level="2" pageId="1" pageNumber="64" reason="3">
<emphasis id="B948DD6FFFCFFFAE780BC423FC25FA91" bold="true" box="[824,970,1362,1386]" pageId="1" pageNumber="64">The protocol</emphasis>
</heading>
</paragraph>
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1 ml of 70% alcohol was added to the test tube after 0.2 ml of tissue homogenate was pipetted out. For ten minutes, the tubes were centrifuged at 5,000 rpm. After discarding the supernatant, 1 ml of 
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NaOH was used to dissolve the precipitate. 5 ml of Alkaline Copper Reagent was added, and it was left at room temperature for ten minutes. The Folin-Ciocalteu phenol reagent was added after ten minutes, and it was left in the dark for thirty minutes. The UV-Vis Spectrophotometer was used to measure the absorbance at 
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against a blank for the reagent. Simultaneously, a series of graded protein standard volumes were run. The data are given as milligrams of protein per gram of tissue weight that is moist.
</paragraph>
</subSubSection>
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<paragraph id="8B83017DFFCFFFAE7B4EC654FA59F8C1" blockId="1.[125,1462,1828,1850]" box="[125,1462,1828,1850]" pageId="1" pageNumber="64">
<emphasis id="B948DD6FFFCFFFAE7B4EC654FF3DF8C1" bold="true" box="[125,210,1829,1850]" pageId="1" pageNumber="64">Table 1:</emphasis>
Morphological and behavioural changes in fishes when exposed to doses (5 ppm and 10 ppm) of CdCl 
<subScript id="17B80338FFCFFFAE7F83C65AFB57F8C1" attach="left" box="[1200,1208,1835,1850]" fontSize="6" pageId="1" pageNumber="64">2</subScript>
at different exposure time
</paragraph>
</caption>
<paragraph id="8B83017DFFCFFFAE7B58C620FA9EF7C9" pageId="1" pageNumber="64">
<table id="F93CF3DDFFCF00507B58C620FA28F7C9" box="[107,1479,1873,2098]" colsContinueIn="2.[151,1393,135,464]" gridcols="8" gridrows="8" pageId="1" pageNumber="64">
<tr id="350C033FFFCF00507B58C620FA28F89F" box="[107,1479,1873,1892]" gridrow="0" isHeader="true" pageId="1" pageNumber="64">
<th id="76DD6A43FFCF00507B58C620FF19F89F" box="[107,246,1873,1892]" gridcol="0" gridrow="0" pageId="1" pageNumber="64">Exposure Time</th>
<th id="76DD6A43FFCF00507BCDC620FED0F89F" box="[254,319,1873,1892]" gridcol="1" gridrow="0" pageId="1" pageNumber="64">Dose</th>
<th id="76DD6A43FFCF00507A74C620FE34F89F" box="[327,475,1873,1892]" gridcol="2" gridrow="0" pageId="1" pageNumber="64">Colour Changes</th>
<th id="76DD6A43FFCF00507AD5C620FD61F89F" box="[486,654,1873,1892]" gridcol="3" gridrow="0" pageId="1" pageNumber="64">Opercular activity</th>
<th id="76DD6A43FFCF005079AFC620FC4DF89F" box="[668,930,1873,1892]" gridcol="4" gridrow="0" pageId="1" pageNumber="64">Bottom to surface movement</th>
<th id="76DD6A43FFCF00507882C620FBB9F89F" box="[945,1110,1873,1892]" gridcol="5" gridrow="0" pageId="1" pageNumber="64">Resting at Bottom</th>
<th id="76DD6A43FFCF00507F52C620FAECF89F" box="[1121,1283,1873,1892]" gridcol="6" gridrow="0" pageId="1" pageNumber="64">Mucous Secretion</th>
<th id="76DD6A43FFCF00507E23C620FA28F89F" box="[1296,1479,1873,1892]" gridcol="7" gridrow="0" pageId="1" pageNumber="64">
<emphasis id="B948DD6FFFCFFFAE7E23C620FA28F89F" bold="true" box="[1296,1479,1873,1892]" pageId="1" pageNumber="64">Loss of Equilibrium</emphasis>
</th>
</tr>
<tr id="350C033FFFCF00507B58C61BFA28F886" box="[107,1479,1898,1917]" gridrow="1" pageId="1" pageNumber="64">
<th id="76DD6A43FFCF00507B58C61BFA28F886" box="[107,1479,1898,1917]" colspan="8" colspanRight="7" gridcol="0" gridrow="1" pageId="1" pageNumber="64">Control</th>
</tr>
<tr id="350C033FFFCF00507B58C6F5FA28F86C" box="[107,1479,1924,1943]" gridrow="2" pageId="1" pageNumber="64">
<th id="76DD6A43FFCF00507B58C6F5FF19F84A" box="[107,246,1924,1969]" gridcol="0" gridrow="2" pageId="1" pageNumber="64" rowspan="2" rowspanBelow="1">Day 1</th>
<td id="76DD6A43FFCF00507BCDC6F5FED0F86C" box="[254,319,1924,1943]" gridcol="1" gridrow="2" pageId="1" pageNumber="64">SL-I</td>
<td id="76DD6A43FFCF00507A74C6F5FE34F86C" box="[327,475,1924,1943]" gridcol="2" gridrow="2" pageId="1" pageNumber="64">=</td>
<td id="76DD6A43FFCF00507AD5C6F5FD61F86C" box="[486,654,1924,1943]" gridcol="3" gridrow="2" pageId="1" pageNumber="64">*</td>
<td id="76DD6A43FFCF005079AFC6F5FC4DF86C" box="[668,930,1924,1943]" gridcol="4" gridrow="2" pageId="1" pageNumber="64">+</td>
<td id="76DD6A43FFCF00507882C6F5FBB9F86C" box="[945,1110,1924,1943]" gridcol="5" gridrow="2" pageId="1" pageNumber="64">=</td>
<td id="76DD6A43FFCF00507F52C6F5FAECF86C" box="[1121,1283,1924,1943]" gridcol="6" gridrow="2" pageId="1" pageNumber="64">+</td>
<td id="76DD6A43FFCF00507E23C6F5FA28F86C" box="[1296,1479,1924,1943]" gridcol="7" gridrow="2" pageId="1" pageNumber="64">+</td>
</tr>
<tr id="350C033FFFCF00507B58C6EFFA28F84A" box="[107,1479,1950,1969]" gridrow="3" pageId="1" pageNumber="64">
<td id="76DD6A43FFCF00507BCDC6EFFED0F84A" box="[254,319,1950,1969]" gridcol="1" gridrow="3" pageId="1" pageNumber="64">SL-II</td>
<td id="76DD6A43FFCF00507A74C6EFFE34F84A" box="[327,475,1950,1969]" gridcol="2" gridrow="3" pageId="1" pageNumber="64">*</td>
<td id="76DD6A43FFCF00507AD5C6EFFD61F84A" box="[486,654,1950,1969]" gridcol="3" gridrow="3" pageId="1" pageNumber="64">*</td>
<td id="76DD6A43FFCF005079AFC6EFFC4DF84A" box="[668,930,1950,1969]" gridcol="4" gridrow="3" pageId="1" pageNumber="64">+</td>
<td id="76DD6A43FFCF00507882C6EFFBB9F84A" box="[945,1110,1950,1969]" gridcol="5" gridrow="3" pageId="1" pageNumber="64">=</td>
<td id="76DD6A43FFCF00507F52C6EFFAECF84A" box="[1121,1283,1950,1969]" gridcol="6" gridrow="3" pageId="1" pageNumber="64">+</td>
<td id="76DD6A43FFCF00507E23C6EFFA28F84A" box="[1296,1479,1950,1969]" gridcol="7" gridrow="3" pageId="1" pageNumber="64">*</td>
</tr>
<tr id="350C033FFFCF00507B58C6C9FA28F830" box="[107,1479,1976,1995]" gridrow="4" pageId="1" pageNumber="64">
<th id="76DD6A43FFCF00507B58C6C9FA28F830" box="[107,1479,1976,1995]" colspan="8" colspanRight="7" gridcol="0" gridrow="4" pageId="1" pageNumber="64">Control</th>
</tr>
<tr id="350C033FFFCF00507B58C6A3FA28F81E" box="[107,1479,2002,2021]" gridrow="5" pageId="1" pageNumber="64">
<th id="76DD6A43FFCF00507B58C6A3FF19F805" box="[107,246,2002,2046]" gridcol="0" gridrow="5" pageId="1" pageNumber="64" rowspan="2" rowspanBelow="1">Day 2</th>
<td id="76DD6A43FFCF00507BCDC6A3FED0F81E" box="[254,319,2002,2021]" gridcol="1" gridrow="5" pageId="1" pageNumber="64">SL-I</td>
<td id="76DD6A43FFCF00507A74C6A3FE34F81E" box="[327,475,2002,2021]" gridcol="2" gridrow="5" pageId="1" pageNumber="64">+</td>
<td id="76DD6A43FFCF00507AD5C6A3FD61F81E" box="[486,654,2002,2021]" gridcol="3" gridrow="5" pageId="1" pageNumber="64">=</td>
<td id="76DD6A43FFCF005079AFC6A3FC4DF81E" box="[668,930,2002,2021]" gridcol="4" gridrow="5" pageId="1" pageNumber="64">=</td>
<td id="76DD6A43FFCF00507882C6A3FBB9F81E" box="[945,1110,2002,2021]" gridcol="5" gridrow="5" pageId="1" pageNumber="64">*</td>
<td id="76DD6A43FFCF00507F52C6A3FAECF81E" box="[1121,1283,2002,2021]" gridcol="6" gridrow="5" pageId="1" pageNumber="64">+</td>
<td id="76DD6A43FFCF00507E23C6A3FA28F81E" box="[1296,1479,2002,2021]" gridcol="7" gridrow="5" pageId="1" pageNumber="64">=</td>
</tr>
<tr id="350C033FFFCF00507B58C69AFA28F805" box="[107,1479,2027,2046]" gridrow="6" pageId="1" pageNumber="64">
<td id="76DD6A43FFCF00507BCDC69AFED0F805" box="[254,319,2027,2046]" gridcol="1" gridrow="6" pageId="1" pageNumber="64">SL-II</td>
<td id="76DD6A43FFCF00507A74C69AFE34F805" box="[327,475,2027,2046]" gridcol="2" gridrow="6" pageId="1" pageNumber="64">=</td>
<td id="76DD6A43FFCF00507AD5C69AFD61F805" box="[486,654,2027,2046]" gridcol="3" gridrow="6" pageId="1" pageNumber="64">=</td>
<td id="76DD6A43FFCF005079AFC69AFC4DF805" box="[668,930,2027,2046]" gridcol="4" gridrow="6" pageId="1" pageNumber="64">*</td>
<td id="76DD6A43FFCF00507882C69AFBB9F805" box="[945,1110,2027,2046]" gridcol="5" gridrow="6" pageId="1" pageNumber="64">*</td>
<td id="76DD6A43FFCF00507F52C69AFAECF805" box="[1121,1283,2027,2046]" gridcol="6" gridrow="6" pageId="1" pageNumber="64">+</td>
<td id="76DD6A43FFCF00507E23C69AFA28F805" box="[1296,1479,2027,2046]" gridcol="7" gridrow="6" pageId="1" pageNumber="64">*</td>
</tr>
<tr id="350C033FFFCF00507B58C974FA28F7C9" box="[107,1479,2053,2098]" gridrow="7" pageId="1" pageNumber="64">
<th id="76DD6A43FFCF00507B58C974FF19F7C9" box="[107,246,2053,2098]" gridcol="0" gridrow="7" pageId="1" pageNumber="64">Day 3</th>
<td id="76DD6A43FFCF00507BCDC974FED0F7C9" box="[254,319,2053,2098]" gridcol="1" gridrow="7" pageId="1" pageNumber="64">Control SL-I</td>
<td id="76DD6A43FFCF00507A74C974FE34F7C9" box="[327,475,2053,2098]" gridcol="2" gridrow="7" pageId="1" pageNumber="64">+</td>
<td id="76DD6A43FFCF00507AD5C974FD61F7C9" box="[486,654,2053,2098]" gridcol="3" gridrow="7" pageId="1" pageNumber="64">*</td>
<td id="76DD6A43FFCF005079AFC974FC4DF7C9" box="[668,930,2053,2098]" gridcol="4" gridrow="7" pageId="1" pageNumber="64">=</td>
<td id="76DD6A43FFCF00507882C974FBB9F7C9" box="[945,1110,2053,2098]" gridcol="5" gridrow="7" pageId="1" pageNumber="64">=</td>
<td id="76DD6A43FFCF00507F52C974FAECF7C9" box="[1121,1283,2053,2098]" gridcol="6" gridrow="7" pageId="1" pageNumber="64">+</td>
<td id="76DD6A43FFCF00507E23C974FA28F7C9" box="[1296,1479,2053,2098]" gridcol="7" gridrow="7" pageId="1" pageNumber="64">=</td>
</tr>
</table>
</paragraph>
<paragraph id="8B83017DFFCCFFAD7A3BC1F6FA9EFE2B" pageId="2" pageNumber="65">
<table id="F93CF3DDFFCC00507BA4C1F6FA9EFE2B" box="[151,1393,135,464]" colsContinueFrom="1.[107,1479,1873,2098]" gridcols="8" gridrows="13" pageId="2" pageNumber="65">
<tr id="350C033FFFCC00507BA4C1F6FA9EFF61" box="[151,1393,135,154]" gridrow="0" pageId="2" pageNumber="65" rowspan-0="1">
<th id="76DD6A43FFCC00507BCDC1F6FED0FF61" box="[254,319,135,154]" gridcol="1" gridrow="0" pageId="2" pageNumber="65">SL-II</th>
<th id="76DD6A43FFCC00507AB8C1F6FE78FF61" box="[395,407,135,154]" gridcol="2" gridrow="0" pageId="2" pageNumber="65">=</th>
<th id="76DD6A43FFCC00507900C1F6FDD0FF61" box="[563,575,135,154]" gridcol="3" gridrow="0" pageId="2" pageNumber="65">*</th>
<th id="76DD6A43FFCC0050782AC1F6FCCAFF61" box="[793,805,135,154]" gridcol="4" gridrow="0" pageId="2" pageNumber="65">*</th>
<th id="76DD6A43FFCC005078CEC1F6FBE6FF61" box="[1021,1033,135,154]" gridcol="5" gridrow="0" pageId="2" pageNumber="65">=</th>
<th id="76DD6A43FFCC00507F9FC1F6FB57FF61" box="[1196,1208,135,154]" gridcol="6" gridrow="0" pageId="2" pageNumber="65">+</th>
<th id="76DD6A43FFCC00507E56C1F6FA9EFF61" box="[1381,1393,135,154]" gridcol="7" gridrow="0" pageId="2" pageNumber="65">*</th>
</tr>
<tr id="350C033FFFCC00507BA4C1D1FA9EFF48" box="[151,1393,160,179]" gridrow="1" pageId="2" pageNumber="65" rowspan-2="1" rowspan-3="1" rowspan-4="1" rowspan-5="1" rowspan-6="1" rowspan-7="1">
<th id="76DD6A43FFCC00507BA4C1D1FF24FF1C" box="[151,203,160,231]" gridcol="0" gridrow="1" pageId="2" pageNumber="65" rowspan="3" rowspanBelow="2">Day 4</th>
<td id="76DD6A43FFCC00507BCDC1D1FED0FF48" box="[254,319,160,179]" gridcol="1" gridrow="1" pageId="2" pageNumber="65">Control</td>
</tr>
<tr id="350C033FFFCC00507BA4C1CBFA9EFF36" box="[151,1393,186,205]" gridrow="2" pageId="2" pageNumber="65">
<td id="76DD6A43FFCC00507BCDC1CBFED0FF36" box="[254,319,186,205]" gridcol="1" gridrow="2" pageId="2" pageNumber="65">SL-I</td>
<td id="76DD6A43FFCC00507AB8C1CBFE78FF36" box="[395,407,186,205]" gridcol="2" gridrow="2" pageId="2" pageNumber="65">+</td>
<td id="76DD6A43FFCC00507900C1CBFDD0FF36" box="[563,575,186,205]" gridcol="3" gridrow="2" pageId="2" pageNumber="65">*</td>
<td id="76DD6A43FFCC0050782AC1CBFCCAFF36" box="[793,805,186,205]" gridcol="4" gridrow="2" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC005078CEC1CBFBE6FF36" box="[1021,1033,186,205]" gridcol="5" gridrow="2" pageId="2" pageNumber="65">*</td>
<td id="76DD6A43FFCC00507F9FC1CBFB57FF36" box="[1196,1208,186,205]" gridcol="6" gridrow="2" pageId="2" pageNumber="65">+</td>
<td id="76DD6A43FFCC00507E56C1CBFA9EFF36" box="[1381,1393,186,205]" gridcol="7" gridrow="2" pageId="2" pageNumber="65">*</td>
</tr>
<tr id="350C033FFFCC00507BA4C1A5FA9EFF1C" box="[151,1393,212,231]" gridrow="3" pageId="2" pageNumber="65">
<td id="76DD6A43FFCC00507BCDC1A5FED0FF1C" box="[254,319,212,231]" gridcol="1" gridrow="3" pageId="2" pageNumber="65">SL-II</td>
<td id="76DD6A43FFCC00507AB8C1A5FE78FF1C" box="[395,407,212,231]" gridcol="2" gridrow="3" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC00507900C1A5FDD0FF1C" box="[563,575,212,231]" gridcol="3" gridrow="3" pageId="2" pageNumber="65">*</td>
<td id="76DD6A43FFCC0050782AC1A5FCCAFF1C" box="[793,805,212,231]" gridcol="4" gridrow="3" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC005078CEC1A5FBE6FF1C" box="[1021,1033,212,231]" gridcol="5" gridrow="3" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC00507F9FC1A5FB57FF1C" box="[1196,1208,212,231]" gridcol="6" gridrow="3" pageId="2" pageNumber="65">+</td>
<td id="76DD6A43FFCC00507E56C1A5FA9EFF1C" box="[1381,1393,212,231]" gridcol="7" gridrow="3" pageId="2" pageNumber="65">*</td>
</tr>
<tr id="350C033FFFCC00507BA4C19FFA9EFEFA" box="[151,1393,238,257]" gridrow="4" pageId="2" pageNumber="65" rowspan-2="1" rowspan-3="1" rowspan-4="1" rowspan-5="1" rowspan-6="1" rowspan-7="1">
<th id="76DD6A43FFCC00507BA4C19FFF24FECE" box="[151,203,238,309]" gridcol="0" gridrow="4" pageId="2" pageNumber="65" rowspan="3" rowspanBelow="2">Day 5</th>
<td id="76DD6A43FFCC00507BCDC19FFED0FEFA" box="[254,319,238,257]" gridcol="1" gridrow="4" pageId="2" pageNumber="65">Control</td>
</tr>
<tr id="350C033FFFCC00507BA4C079FA9EFEE0" box="[151,1393,264,283]" gridrow="5" pageId="2" pageNumber="65">
<td id="76DD6A43FFCC00507BCDC079FED0FEE0" box="[254,319,264,283]" gridcol="1" gridrow="5" pageId="2" pageNumber="65">SL-I</td>
<td id="76DD6A43FFCC00507AB8C079FE78FEE0" box="[395,407,264,283]" gridcol="2" gridrow="5" pageId="2" pageNumber="65">+</td>
<td id="76DD6A43FFCC00507900C079FDD0FEE0" box="[563,575,264,283]" gridcol="3" gridrow="5" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC0050782AC079FCCAFEE0" box="[793,805,264,283]" gridcol="4" gridrow="5" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC005078CEC079FBE6FEE0" box="[1021,1033,264,283]" gridcol="5" gridrow="5" pageId="2" pageNumber="65">*</td>
<td id="76DD6A43FFCC00507F9FC079FB57FEE0" box="[1196,1208,264,283]" gridcol="6" gridrow="5" pageId="2" pageNumber="65">*</td>
<td id="76DD6A43FFCC00507E56C079FA9EFEE0" box="[1381,1393,264,283]" gridcol="7" gridrow="5" pageId="2" pageNumber="65">=</td>
</tr>
<tr id="350C033FFFCC00507BA4C053FA9EFECE" box="[151,1393,290,309]" gridrow="6" pageId="2" pageNumber="65">
<td id="76DD6A43FFCC00507BCDC053FED0FECE" box="[254,319,290,309]" gridcol="1" gridrow="6" pageId="2" pageNumber="65">SL-II</td>
<td id="76DD6A43FFCC00507AB8C053FE78FECE" box="[395,407,290,309]" gridcol="2" gridrow="6" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC00507900C053FDD0FECE" box="[563,575,290,309]" gridcol="3" gridrow="6" pageId="2" pageNumber="65">+</td>
<td id="76DD6A43FFCC0050782AC053FCCAFECE" box="[793,805,290,309]" gridcol="4" gridrow="6" pageId="2" pageNumber="65">*</td>
<td id="76DD6A43FFCC005078CEC053FBE6FECE" box="[1021,1033,290,309]" gridcol="5" gridrow="6" pageId="2" pageNumber="65">+</td>
<td id="76DD6A43FFCC00507F9FC053FB57FECE" box="[1196,1208,290,309]" gridcol="6" gridrow="6" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC00507E56C053FA9EFECE" box="[1381,1393,290,309]" gridcol="7" gridrow="6" pageId="2" pageNumber="65">*</td>
</tr>
<tr id="350C033FFFCC00507BA4C04DFA9EFEB4" box="[151,1393,316,335]" gridrow="7" pageId="2" pageNumber="65" rowspan-2="1" rowspan-3="1" rowspan-4="1" rowspan-5="1" rowspan-6="1" rowspan-7="1">
<th id="76DD6A43FFCC00507BA4C04DFF24FE79" box="[151,203,316,386]" gridcol="0" gridrow="7" pageId="2" pageNumber="65" rowspan="3" rowspanBelow="2">Day 6</th>
<td id="76DD6A43FFCC00507BCDC04DFED0FEB4" box="[254,319,316,335]" gridcol="1" gridrow="7" pageId="2" pageNumber="65">Control</td>
</tr>
<tr id="350C033FFFCC00507BA4C024FA9EFE93" box="[151,1393,341,360]" gridrow="8" pageId="2" pageNumber="65">
<td id="76DD6A43FFCC00507BCDC024FED0FE93" box="[254,319,341,360]" gridcol="1" gridrow="8" pageId="2" pageNumber="65">SL-I</td>
<td id="76DD6A43FFCC00507AB8C024FE78FE93" box="[395,407,341,360]" gridcol="2" gridrow="8" pageId="2" pageNumber="65">+</td>
<td id="76DD6A43FFCC00507900C024FDD0FE93" box="[563,575,341,360]" gridcol="3" gridrow="8" pageId="2" pageNumber="65">*</td>
<td id="76DD6A43FFCC0050782AC024FCCAFE93" box="[793,805,341,360]" gridcol="4" gridrow="8" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC005078CEC024FBE6FE93" box="[1021,1033,341,360]" gridcol="5" gridrow="8" pageId="2" pageNumber="65">+</td>
<td id="76DD6A43FFCC00507F9FC024FB57FE93" box="[1196,1208,341,360]" gridcol="6" gridrow="8" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC00507E56C024FA9EFE93" box="[1381,1393,341,360]" gridcol="7" gridrow="8" pageId="2" pageNumber="65">*</td>
</tr>
<tr id="350C033FFFCC00507BA4C01EFA9EFE79" box="[151,1393,367,386]" gridrow="9" pageId="2" pageNumber="65">
<td id="76DD6A43FFCC00507BCDC01EFED0FE79" box="[254,319,367,386]" gridcol="1" gridrow="9" pageId="2" pageNumber="65">SL-II</td>
<td id="76DD6A43FFCC00507AB8C01EFE78FE79" box="[395,407,367,386]" gridcol="2" gridrow="9" pageId="2" pageNumber="65">+</td>
<td id="76DD6A43FFCC00507900C01EFDD0FE79" box="[563,575,367,386]" gridcol="3" gridrow="9" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC0050782AC01EFCCAFE79" box="[793,805,367,386]" gridcol="4" gridrow="9" pageId="2" pageNumber="65">+</td>
<td id="76DD6A43FFCC005078CEC01EFBE6FE79" box="[1021,1033,367,386]" gridcol="5" gridrow="9" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC00507F9FC01EFB57FE79" box="[1196,1208,367,386]" gridcol="6" gridrow="9" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC00507E56C01EFA9EFE79" box="[1381,1393,367,386]" gridcol="7" gridrow="9" pageId="2" pageNumber="65">+</td>
</tr>
<tr id="350C033FFFCC00507BA4C0F8FA9EFE67" box="[151,1393,393,412]" gridrow="10" pageId="2" pageNumber="65" rowspan-2="1" rowspan-3="1" rowspan-4="1" rowspan-5="1" rowspan-6="1" rowspan-7="1">
<th id="76DD6A43FFCC00507BA4C0F8FF24FE2B" box="[151,203,393,464]" gridcol="0" gridrow="10" pageId="2" pageNumber="65" rowspan="3" rowspanBelow="2">Day 7</th>
<td id="76DD6A43FFCC00507BCDC0F8FED0FE67" box="[254,319,393,412]" gridcol="1" gridrow="10" pageId="2" pageNumber="65">Control</td>
</tr>
<tr id="350C033FFFCC00507BA4C0D2FA9EFE4D" box="[151,1393,419,438]" gridrow="11" pageId="2" pageNumber="65">
<td id="76DD6A43FFCC00507BCDC0D2FED0FE4D" box="[254,319,419,438]" gridcol="1" gridrow="11" pageId="2" pageNumber="65">SL-I</td>
<td id="76DD6A43FFCC00507AB8C0D2FE78FE4D" box="[395,407,419,438]" gridcol="2" gridrow="11" pageId="2" pageNumber="65">*</td>
<td id="76DD6A43FFCC00507900C0D2FDD0FE4D" box="[563,575,419,438]" gridcol="3" gridrow="11" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC0050782AC0D2FCCAFE4D" box="[793,805,419,438]" gridcol="4" gridrow="11" pageId="2" pageNumber="65">+</td>
<td id="76DD6A43FFCC005078CEC0D2FBE6FE4D" box="[1021,1033,419,438]" gridcol="5" gridrow="11" pageId="2" pageNumber="65">+</td>
<td id="76DD6A43FFCC00507F9FC0D2FB57FE4D" box="[1196,1208,419,438]" gridcol="6" gridrow="11" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC00507E56C0D2FA9EFE4D" box="[1381,1393,419,438]" gridcol="7" gridrow="11" pageId="2" pageNumber="65">=</td>
</tr>
<tr id="350C033FFFCC00507BA4C0CCFA9EFE2B" box="[151,1393,445,464]" gridrow="12" pageId="2" pageNumber="65">
<td id="76DD6A43FFCC00507BCDC0CCFED0FE2B" box="[254,319,445,464]" gridcol="1" gridrow="12" pageId="2" pageNumber="65">SL-II</td>
<td id="76DD6A43FFCC00507AB8C0CCFE78FE2B" box="[395,407,445,464]" gridcol="2" gridrow="12" pageId="2" pageNumber="65">+</td>
<td id="76DD6A43FFCC00507900C0CCFDD0FE2B" box="[563,575,445,464]" gridcol="3" gridrow="12" pageId="2" pageNumber="65">=</td>
<td id="76DD6A43FFCC0050782AC0CCFCCAFE2B" box="[793,805,445,464]" gridcol="4" gridrow="12" pageId="2" pageNumber="65">*</td>
<td id="76DD6A43FFCC005078CEC0CCFBE6FE2B" box="[1021,1033,445,464]" gridcol="5" gridrow="12" pageId="2" pageNumber="65">+</td>
<td id="76DD6A43FFCC00507F9FC0CCFB57FE2B" box="[1196,1208,445,464]" gridcol="6" gridrow="12" pageId="2" pageNumber="65">*</td>
<td id="76DD6A43FFCC00507E56C0CCFA9EFE2B" box="[1381,1393,445,464]" gridcol="7" gridrow="12" pageId="2" pageNumber="65">+</td>
</tr>
</table>
</paragraph>
<tableNote id="76DA00F3FFCCFFAD7B53C0A6FD61FDBB" pageId="2" pageNumber="65" targetBox="[151,1393,135,464]" targetPageId="2">
<paragraph id="8B83017DFFCCFFAD7B53C0A6FE6AFE16" blockId="2.[96,654,471,576]" box="[96,389,471,493]" pageId="2" pageNumber="65">SL-I = 5 ppm; SL-II = 10 ppm</paragraph>
<paragraph id="8B83017DFFCCFFAD7B53C082FDC4FDF2" blockId="2.[96,654,471,576]" box="[96,555,499,521]" pageId="2" pageNumber="65">“=” indicates no change or no increase/decrease</paragraph>
<paragraph id="8B83017DFFCCFFAD7B53C37FFD63FDDF" blockId="2.[96,654,471,576]" box="[96,652,526,548]" pageId="2" pageNumber="65">“+” indicates occurrence of changes or increase in change</paragraph>
<paragraph id="8B83017DFFCCFFAD7B53C35BFD61FDBB" blockId="2.[96,654,471,576]" box="[96,654,554,576]" pageId="2" pageNumber="65">“*” indicates occurrence of changes or decrease in change</paragraph>
</tableNote>
<caption id="DF4351F5FFCCFFAD7B99C314FA66FD81" ID-Table-UUID="DF4351F5FFCCFFAD7B99C314FA66FD81" box="[170,1417,612,634]" httpUri="http://table.plazi.org/id/DF4351F5FFCCFFAD7B99C314FA66FD81" pageId="2" pageNumber="65" startId="2.[170,229,613,634]" targetBox="[168,1362,658,765]" targetIsTable="true" targetPageId="2" targetType="table">
<paragraph id="8B83017DFFCCFFAD7B99C314FA66FD81" blockId="2.[170,1417,612,634]" box="[170,1417,612,634]" pageId="2" pageNumber="65">
<emphasis id="B948DD6FFFCCFFAD7B99C314FF11FD81" bold="true" box="[170,254,613,634]" pageId="2" pageNumber="65">Table 2:</emphasis>
Protein Content of the tissues (gill and liver) of 
<taxonomicName id="4C3C7AFEFFCCFFAD79E3C315FCAFFD81" box="[720,832,612,634]" family="Channidae" genus="Channa" kingdom="Animalia" order="Perciformes" pageId="2" pageNumber="65" phylum="Chordata" rank="species" species="punctata">
<emphasis id="B948DD6FFFCCFFAD79E3C315FCAFFD81" box="[720,832,612,634]" italics="true" pageId="2" pageNumber="65">C. punctata</emphasis>
</taxonomicName>
at different concentrations of CdCl 
<subScript id="17B80338FFCCFFAD7FA7C31AFB73FD81" attach="left" box="[1172,1180,619,634]" fontSize="6" pageId="2" pageNumber="65">2</subScript>
after 7 days of exposure
</paragraph>
</caption>
<paragraph id="8B83017DFFCCFFAD7BE5C3E3FABDFD06" pageId="2" pageNumber="65">
<table id="F93CF3DDFFCC00507B9BC3E3FABDFD06" box="[168,1362,658,765]" gridcols="4" gridrows="4" pageId="2" pageNumber="65">
<tr id="350C033FFFCC00507B9BC3E3FABDFD5C" box="[168,1362,658,679]" gridrow="0" isHeader="true" pageId="2" pageNumber="65">
<th id="76DD6A43FFCC00507B9BC3E3FE95FD5C" box="[168,378,658,679]" gridcol="0" gridrow="0" pageId="2" pageNumber="65">
<emphasis id="B948DD6FFFCCFFAD7BE5C3E3FEA2FD5C" bold="true" box="[214,333,658,679]" pageId="2" pageNumber="65">Treatments</emphasis>
</th>
<th id="76DD6A43FFCC0050790EC3E3FD75FD5C" box="[573,666,658,679]" gridcol="1" gridrow="0" pageId="2" pageNumber="65">
<emphasis id="B948DD6FFFCCFFAD790EC3E3FD75FD5C" bold="true" box="[573,666,658,679]" pageId="2" pageNumber="65">Duration</emphasis>
</th>
<th id="76DD6A43FFCC005078A5C3E3FC17FD5C" box="[918,1016,658,679]" gridcol="2" gridrow="0" pageId="2" pageNumber="65">
<emphasis id="B948DD6FFFCCFFAD7887C3E3FC34FD5C" bold="true" box="[948,987,658,679]" pageId="2" pageNumber="65">Gill</emphasis>
</th>
<th id="76DD6A43FFCC00507FC2C3E3FABDFD5C" box="[1265,1362,658,679]" gridcol="3" gridrow="0" pageId="2" pageNumber="65">
<emphasis id="B948DD6FFFCCFFAD7E35C3E3FAD1FD5C" bold="true" box="[1286,1342,658,679]" pageId="2" pageNumber="65">Liver</emphasis>
</th>
</tr>
<tr id="350C033FFFCC00507B9BC3DFFABDFD3F" box="[168,1362,686,708]" gridrow="1" pageId="2" pageNumber="65">
<th id="76DD6A43FFCC00507B9BC3DFFE95FD3F" box="[168,378,686,708]" gridcol="0" gridrow="1" pageId="2" pageNumber="65">5 ppm CdCl2 (mg/L)</th>
<td id="76DD6A43FFCC0050790EC3DFFD75FD3F" box="[573,666,686,708]" gridcol="1" gridrow="1" pageId="2" pageNumber="65">7 Days</td>
<td id="76DD6A43FFCC005078A5C3DFFC17FD3F" box="[918,1016,686,708]" gridcol="2" gridrow="1" pageId="2" pageNumber="65">70.3±0.25</td>
<td id="76DD6A43FFCC00507FC2C3DFFABDFD3F" box="[1265,1362,686,708]" gridcol="3" gridrow="1" pageId="2" pageNumber="65">79.3±0.20</td>
</tr>
<tr id="350C033FFFCC00507B9BC3BBFABDFD1B" box="[168,1362,714,736]" gridrow="2" pageId="2" pageNumber="65">
<th id="76DD6A43FFCC00507B9BC3BBFE95FD1B" box="[168,378,714,736]" gridcol="0" gridrow="2" pageId="2" pageNumber="65">10 ppm CdCl2 (mg/L)</th>
<td id="76DD6A43FFCC0050790EC3BBFD75FD1B" box="[573,666,714,736]" gridcol="1" gridrow="2" pageId="2" pageNumber="65">7 Days</td>
<td id="76DD6A43FFCC005078A5C3BBFC17FD1B" box="[918,1016,714,736]" gridcol="2" gridrow="2" pageId="2" pageNumber="65">65.3±0.30</td>
<td id="76DD6A43FFCC00507FC2C3BBFABDFD1B" box="[1265,1362,714,736]" gridcol="3" gridrow="2" pageId="2" pageNumber="65">71.3±0.25</td>
</tr>
<tr id="350C033FFFCC00507B9BC396FABDFD06" box="[168,1362,743,765]" gridrow="3" pageId="2" pageNumber="65">
<th id="76DD6A43FFCC00507B9BC396FE95FD06" box="[168,378,743,765]" gridcol="0" gridrow="3" pageId="2" pageNumber="65">Control</th>
<td id="76DD6A43FFCC0050790EC396FD75FD06" box="[573,666,743,765]" gridcol="1" gridrow="3" pageId="2" pageNumber="65">7 Days</td>
<td id="76DD6A43FFCC005078A5C396FC17FD06" box="[918,1016,743,765]" gridcol="2" gridrow="3" pageId="2" pageNumber="65">82.5±0.20</td>
<td id="76DD6A43FFCC00507FC2C396FABDFD06" box="[1265,1362,743,765]" gridcol="3" gridrow="3" pageId="2" pageNumber="65">89.7±0.15</td>
</tr>
</table>
</paragraph>
<caption id="DF4351F5FFCCFFAD7BFCC75EFA8BF9BF" ID-DOI="http://doi.org/10.5281/zenodo.12550285" ID-Zenodo-Dep="12550285" box="[207,1380,1583,1605]" httpUri="https://zenodo.org/record/12550285/files/figure.png" pageId="2" pageNumber="65" startId="2.[207,240,1583,1604]" targetBox="[271,1336,816,1551]" targetPageId="2" targetType="figure">
<paragraph id="8B83017DFFCCFFAD7BFCC75EFA8BF9BF" blockId="2.[207,1380,1583,1605]" box="[207,1380,1583,1605]" pageId="2" pageNumber="65">
<emphasis id="B948DD6FFFCCFFAD7BFCC75EFEE5F9BF" bold="true" box="[207,266,1583,1604]" pageId="2" pageNumber="65">Fig 1:</emphasis>
Protein Content of the tissues (gill and liver) of 
<taxonomicName id="4C3C7AFEFFCCFFAD79EFC75EFCA3F9BE" box="[732,844,1583,1605]" family="Channidae" genus="Channa" kingdom="Animalia" order="Perciformes" pageId="2" pageNumber="65" phylum="Chordata" rank="species" species="punctata">
<emphasis id="B948DD6FFFCCFFAD79EFC75EFCA3F9BE" box="[732,844,1583,1605]" italics="true" pageId="2" pageNumber="65">C. punctata</emphasis>
</taxonomicName>
at 5 ppm and 10 ppm of CdCl 
<subScript id="17B80338FFCCFFAD7F43C747FB97F9BE" attach="left" box="[1136,1144,1590,1605]" fontSize="6" pageId="2" pageNumber="65">2</subScript>
after 7 days of exposure
</paragraph>
</caption>
<subSubSection id="C32652F6FFCCFFAD7B53C71AFB3DF84F" pageId="2" pageNumber="65" type="discussion">
<paragraph id="8B83017DFFCCFFAD7B53C71AFE8CF978" blockId="2.[96,355,1643,1667]" box="[96,355,1643,1667]" pageId="2" pageNumber="65">
<heading id="D0CBB611FFCCFFAD7B53C71AFE8CF978" bold="true" box="[96,355,1643,1667]" fontSize="36" level="1" pageId="2" pageNumber="65" reason="1">
<emphasis id="B948DD6FFFCCFFAD7B53C71AFE8CF978" bold="true" box="[96,355,1643,1667]" pageId="2" pageNumber="65">Results and Discussion</emphasis>
</heading>
</paragraph>
<paragraph id="8B83017DFFCCFFAD7B53C7F8FC5BF944" blockId="2.[96,763,1673,2095]" lastBlockId="2.[824,1491,1642,1972]" pageId="2" pageNumber="65">
The present study indicated that the heavy metal Cadmium Chloride (CdCl 
<subScript id="17B80338FFCCFFAD7A3BC7C0FEFEF944" attach="left" box="[264,273,1713,1727]" fontSize="6" pageId="2" pageNumber="65">2</subScript>
) has a dreadful effect on the freshwater fish 
<taxonomicName id="4C3C7AFEFFCCFFAD7B53C7B7FE98F925" authority="(Bloch)" baseAuthorityName="Bloch" box="[96,375,1734,1758]" family="Channidae" genus="Channa" kingdom="Animalia" order="Perciformes" pageId="2" pageNumber="65" phylum="Chordata" rank="species" species="punctata">
<emphasis id="B948DD6FFFCCFFAD7B53C7B7FEF4F925" box="[96,283,1734,1758]" italics="true" pageId="2" pageNumber="65">Channa punctata</emphasis>
(Bloch)
</taxonomicName>
(Qureshi et al, 2011) 
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[
<bibRefCitation id="EFAD7C8CFFCCFFAD7944C7B5FD6EF929" author="Ahmad B &amp; Qureshi TA &amp; Manohar S &amp; Kaur P &amp; Khaliq R." box="[631,641,1732,1746]" pageId="2" pageNumber="65" pagination="531 - 536" refId="ref2247" refString="3. Ahmad B, Qureshi TA, Manohar S, Kaur P, Khaliq R. Effect of cadmium chloride on the histoarchitecture of liver and kidney of a freshwater Catfish, Clariasbatrachus. International Journal of Environmental Sciences. 2011; 2 (2): 531 - 536." type="journal article" year="2011">3</bibRefCitation>
]
</superScript>
. From the behavioural responses of fish exposed to sub-lethal concentration of the heavy metal showed that they were under stress. The increase in opercular movement and the bottom to upward movement of the fishes to overcome hypoxic condition was seen in the 5 
<superScript id="7C49AC35FFCCFFAD7ABFC62CFE76F890" attach="left" box="[396,409,1885,1899]" fontSize="6" pageId="2" pageNumber="65">th</superScript>
and 7 
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day of exposure. Mucus secretion, starting of depletion of scales, skin lesions on dorsal and lateral side of the body, copious mucus, clumping of gills increased with the increasing concentration of the toxicant (Rastogi, 
<emphasis id="B948DD6FFFCCFFAD7A15C6AAFEBAF809" box="[294,341,2010,2034]" italics="true" pageId="2" pageNumber="65">et al</emphasis>
., 2018) 
<superScript id="7C49AC35FFCCFFAD7A9CC6A9FE2CF81D" attach="right" box="[431,451,2008,2022]" fontSize="6" pageId="2" pageNumber="65">
[
<bibRefCitation id="EFAD7C8CFFCCFFAD7A87C6A9FE51F81D" author="Rastogi S &amp; Yadav P &amp; Singh M." box="[436,446,2008,2022]" pageId="2" pageNumber="65" pagination="66 - 68" refId="ref2344" refString="5. Rastogi S, Yadav P, Singh M. Toxicity of cadmium and its effect on the morphology of the fresh water Teleost, Heteropneustes fossilis (Bloch). International Journal on Biological Sciences. 2018; 9 (1): 66 - 68." type="journal article" year="2018">5</bibRefCitation>
]
</superScript>
. Colour changes of the body from blackish to pale yellowish and restlessness again confirmed the anxiety level of the fishes in the experiment (
<tableCitation id="C6BE34C6FFCCFFAD780CC71BFC76F979" box="[831,921,1642,1666]" captionStart="Table 1" captionStartId="1.[125,184,1829,1850]" captionTargetBox="[107,1479,1873,2098]" captionTargetId="graphics-840@1.[102,1485,1866,2100]" captionTargetPageId="1" captionText="Table 1: Morphological and behavioural changes in fishes when exposed to doses (5 ppm and 10 ppm) of CdCl2 at different exposure time" httpUri="http://table.plazi.org/id/DF4351F5FFCFFFAE7B4EC654FA59F8C1" pageId="2" pageNumber="65" tableUuid="DF4351F5FFCFFFAE7B4EC654FA59F8C1">Table 1</tableCitation>
). Also many visible lesions were observed near operculum, mouth, pectoral and pelvic fins and also near the caudal fins.
</paragraph>
<paragraph id="8B83017DFFCCFFAD780BC7B7FB3DF84F" blockId="2.[824,1491,1642,1972]" pageId="2" pageNumber="65">
Also from the total protein content assay, it was observed that the gills and liver tissues when exposed to sub lethal heavy metal concentration of CdCl 
<subScript id="17B80338FFCCFFAD7F4CC67CFB67F8E0" attach="left" box="[1151,1160,1805,1819]" fontSize="6" pageId="2" pageNumber="65">2</subScript>
(5ppm and 10ppm) showed gradual decline in the protein content with the increase in exposure time, which further confirms the toxicity of the heavy metal (
<tableCitation id="C6BE34C6FFCCFFAD78E6C62EFBDDF88C" box="[981,1074,1887,1911]" captionStart="Table 2" captionStartId="2.[170,229,613,634]" captionTargetBox="[168,1362,658,765]" captionTargetId="graphics-818@2.[99,1488,650,767]" captionTargetPageId="2" captionText="Table 2: Protein Content of the tissues (gill and liver) of C. punctata at different concentrations of CdCl2 after 7 days of exposure" httpUri="http://table.plazi.org/id/DF4351F5FFCCFFAD7B99C314FA66FD81" pageId="2" pageNumber="65" tableUuid="DF4351F5FFCCFFAD7B99C314FA66FD81">Table 2</tableCitation>
). This result indicates that the vital organs like gills and liver if affected proves lethal in survival of the fishes (Agrahari 
<emphasis id="B948DD6FFFCCFFAD7F1CC6ECFBB1F84F" box="[1071,1118,1948,1972]" italics="true" pageId="2" pageNumber="65">et al</emphasis>
., 2007) 
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[
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]
</superScript>
.
</paragraph>
</subSubSection>
</treatment>
</document>