treatments-xml/data/03/C7/23/03C72374FF88D43FFCD620648C22FCE7.xml

<document id="9F65034C7A28C2ACB3C48E77A9E645B1" ID-DOI="10.1186/s13071-023-05772-7" ID-GBIF-Dataset="ba9ceb1a-d033-4fb1-a682-ab2ded48f85b" ID-ISSN="1756-3305" ID-Zenodo-Dep="11552130" IM.bibliography_approvedBy="guilherme" IM.illustrations_approvedBy="guilherme" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="guilherme" IM.tables_approvedBy="guilherme" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="guilherme" checkinTime="1718040611580" checkinUser="felipe" docAuthor="Ghanei-Motlagh, Reza, Fast, Mark D., Groman, David, Kumar, Gokhlesh, Soliman, Hatem, El-Matbouli, Mansour &amp; Saleh, Mona" docDate="2023" docId="03C72374FF88D43FFCD620648C22FCE7" docLanguage="en" docName="ParasiteVector.16.182.pdf" docOrigin="Parasites &amp; Vectors (182) 16 (1)" docSource="http://dx.doi.org/10.1186/s13071-023-05772-7" docStyle="DocumentStyle:F140E6D4AE1E76E09EF03FD1AEF09E53.3:ParasiteVector.2015-.journal_article" docStyleId="F140E6D4AE1E76E09EF03FD1AEF09E53" docStyleName="ParasiteVector.2015-.journal_article" docStyleVersion="3" docTitle="Huffmanela persica Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh 2023, sp. nov." docType="treatment" docVersion="4" lastPageNumber="10" masterDocId="FFFE5B0CFF8BD436FFFB2449880FFF84" masterDocTitle="Description, molecular identification and pathological lesions of Huffmanela persica sp. nov. (Nematoda: Trichosomoididae: Huffmanelinae) from the daggertooth pike conger Muraenesox cinereus" masterLastPageNumber="20" masterPageNumber="1" pageNumber="4" updateTime="1719327636606" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:title id="C5A63031DD0B2A01BAB6A16C3B28BB67">Description, molecular identification and pathological lesions of Huffmanela persica sp. nov. (Nematoda: Trichosomoididae: Huffmanelinae) from the daggertooth pike conger Muraenesox cinereus</mods:title>
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<mods:namePart id="F1FA77B53B8D24ACF43F8823348A8E8B">Ghanei-Motlagh, Reza</mods:namePart>
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<mods:namePart id="B7816190CE341374383E020EF0B51DB2">Fast, Mark D.</mods:namePart>
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<mods:namePart id="ED84782EA95BB81C472097234A457727">Groman, David</mods:namePart>
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<mods:namePart id="AA6E35F7C4BD586909E37D1D9AA3F554">Soliman, Hatem</mods:namePart>
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<mods:namePart id="88D790B582AF507A1FF8376CDEF1B25F">Saleh, Mona</mods:namePart>
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<taxonomicName id="4C6EE9E1FF88D435FCD620648BFBFBC0" ID-CoL="BJ2FF" authority="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh, 2023" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[813,1012,1069,1092]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="3" pageNumber="4" phylum="Nematoda" rank="species" species="persica" status="sp. nov.">
<emphasis id="B91A4E70FF88D435FCD620648BFBFBC0" bold="true" box="[813,1012,1069,1092]" italics="true" pageId="3" pageNumber="4">Huffmanela persica</emphasis>
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<emphasis id="B91A4E70FF88D435FCD620048C0DFBE0" bold="true" box="[813,1026,1101,1124]" italics="true" pageId="3" pageNumber="4">Taxonomic summary</emphasis>
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<typeStatus id="54D52CC0FF88D435FCD620258B6EFB07" box="[813,865,1132,1155]" pageId="3" pageNumber="4">Type</typeStatus>
definitive host
</emphasis>
: Daggertooth pike conger, 
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<emphasis id="B91A4E70FF88D435FAB020258BC4FB27" italics="true" pageId="3" pageNumber="4">Muraenesox cinereus</emphasis>
(Forsskål)
</taxonomicName>
, 
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, 
<taxonomicName id="4C6EE9E1FF88D435FAFE20C28B5AFB47" family="Muraenesocidae" kingdom="Animalia" order="Anguilliformes" pageId="3" pageNumber="4" phylum="Chordata" rank="family">Muraenesocidae</taxonomicName>
, date of collection, 
<date id="FFD0B4A2FF88D435FBDD20E28CCAFB47" box="[1062,1221,1195,1219]" pageId="3" pageNumber="4" value="2021-02">February 2021</date>
.
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<materialsCitation id="3B06983FFF88D435FCB920858C99FA87" ID-GBIF-Occurrence="4899303301" country="Iran" latitude="28.283333" location="Persian Gulf" longLatPrecision="1233" longitude="51.216667" municipality="Zir Ahak" pageId="3" pageNumber="4" specimenCount="1" stateProvince="Bushehr" typeStatus="holotype">
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<typeStatus id="54D52CC0FF88D435FCB920858B79FB67" box="[834,886,1228,1251]" pageId="3" pageNumber="4">Type</typeStatus>
locality
</emphasis>
: 
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off the coast of 
<collectingMunicipality id="6BB50818FF88D435FAD520828D97FB67" box="[1326,1432,1227,1251]" pageId="3" pageNumber="4">Zir Ahak</collectingMunicipality>
, 
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, 
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(
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, 
<geoCoordinate id="EE5AF4A5FF88D435FBCE20A28C81FA87" box="[1077,1166,1259,1283]" degrees="51" direction="east" minutes="13" orientation="longitude" pageId="3" pageNumber="4" precision="925" value="51.216667">51°13’ E</geoCoordinate>
)
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.
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<paragraph id="8BD19262FF88D432FCB921458AEBF9D4" blockId="3.[813,1437,1034,1475]" lastBlockId="4.[151,775,1528,1936]" lastPageId="4" lastPageNumber="5" pageId="3" pageNumber="4">
<emphasis id="B91A4E70FF88D435FCB921458BF2FAA7" box="[834,1021,1291,1315]" italics="true" pageId="3" pageNumber="4">Site of infection</emphasis>
: Ovary, tunica serosa of stomach (
<figureCitation id="13558EE7FF88D435FCCF21628B85FAC7" box="[820,906,1323,1347]" captionStart="Fig" captionStartId="4.[829,860,1097,1117]" captionTargetBox="[840,1411,238,1082]" captionTargetId="figure-450@4.[837,1412,236,1084]" captionTargetPageId="4" captionText="Fig. 2 Macroscopic and microscopic appearance of fully developed eggs of Huffmanela persica sp. nov. A Grossly visible lesions of eggs previously deposited by adult forms of H.persica sp. nov. in the form of dark spots of various size within the infected tissues (ovary and serosa of stomach) of Muraenesox cinereus. B Wet mount prepared from infected ovary illustrating variously oriented advanced eggs of H.persica sp.nov.within the egg clusters" figureDoi="http://doi.org/10.5281/zenodo.11552134" httpUri="https://zenodo.org/record/11552134/files/figure.png" pageId="3" pageNumber="4">Fig. 2A</figureCitation>
). Conspicuous masses of eggs of 
<taxonomicName id="4C6EE9E1FF88D435FAFF21658D7AFAC7" authority="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh, 2023" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[1284,1397,1324,1347]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="3" pageNumber="4" phylum="Nematoda" rank="species" species="persica" status="sp. nov.">
<emphasis id="B91A4E70FF88D435FAFF21658D7AFAC7" box="[1284,1397,1324,1347]" italics="true" pageId="3" pageNumber="4">H. persica</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A229F30BFF88D435FA8621658B54FAE7" pageId="3" pageNumber="4" rank="species">sp. nov.</taxonomicNameLabel>
were observed in the ovaries of all 13 fish infected. Te egg aggregations were simultaneously found in the serosae of stomach of 3 out of 13 fish infected by the nematode. All fish examined were also infected by larval (viscera) and adult (stomach and intestine) forms of anisakids and raphidascaridids. Adult forms (male and female) of 
<taxonomicName id="4C6EE9E1FF8FD432FEC5227089A2F9D4" authority="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh, 2023" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[318,429,1593,1616]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="persica" status="sp. nov.">
<emphasis id="B91A4E70FF8FD432FEC5227089A2F9D4" box="[318,429,1593,1616]" italics="true" pageId="4" pageNumber="5">H. persica</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A229F30BFF8FD432FE4F22718A06F9CB" box="[436,521,1592,1615]" pageId="4" pageNumber="5" rank="species">sp. nov.</taxonomicNameLabel>
were not observed.
</paragraph>
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<emphasis id="B91A4E70FF88D435FF6C226788ECF9C0" bold="true" box="[151,227,1582,1604]" pageId="3" pageNumber="4">Table 1</emphasis>
List of primers used in this study
</paragraph>
</caption>
<paragraph id="8BD19262FF88D435FF6C22128C11F806" pageId="3" pageNumber="4">
<table id="F96E60C2FF882BC9FF6C22128D99F806" box="[151,1430,1627,1922]" gridcols="4" gridrows="9" pageId="3" pageNumber="4">
<tr id="355E9020FF882BC9FF6C22128D99F9F4" box="[151,1430,1627,1648]" gridrow="0" pageId="3" pageNumber="4">
<th id="768FF95CFF882BC9FF6C22128923F9F4" box="[151,300,1627,1648]" gridcol="0" gridrow="0" pageId="3" pageNumber="4">Molecular marker</th>
<th id="768FF95CFF882BC9FDFB22128A66F9F4" box="[512,617,1627,1648]" gridcol="1" gridrow="0" pageId="3" pageNumber="4">Primer set</th>
<th id="768FF95CFF882BC9FCC622128C7DF9F4" box="[829,1138,1627,1648]" gridcol="2" gridrow="0" pageId="3" pageNumber="4">
Sequence (5 
<emphasis id="B91A4E70FF88D435FC5D22128BA4F9EB" bold="true" box="[934,939,1627,1647]" italics="true" pageId="3" pageNumber="4">′</emphasis>
-3 
<emphasis id="B91A4E70FF88D435FC4722128BCEF9EB" bold="true" box="[956,961,1627,1647]" italics="true" pageId="3" pageNumber="4">′</emphasis>
)
</th>
<th id="768FF95CFF882BC9FAC222128D99F9F4" box="[1337,1430,1627,1648]" gridcol="3" gridrow="0" pageId="3" pageNumber="4">References</th>
</tr>
<tr id="355E9020FF882BC9FF6C22C58D99F924" box="[151,1430,1676,1696]" gridrow="1" pageId="3" pageNumber="4">
<th id="768FF95CFF882BC9FF6C22C58923F924" box="[151,300,1676,1696]" gridcol="0" gridrow="1" pageId="3" pageNumber="4">18S rDNA</th>
<td id="768FF95CFF882BC9FDFB22C58A66F924" box="[512,617,1676,1696]" gridcol="1" gridrow="1" pageId="3" pageNumber="4">Nem_18S_F</td>
<td id="768FF95CFF882BC9FCC622C58C7DF924" box="[829,1138,1676,1696]" gridcol="2" gridrow="1" pageId="3" pageNumber="4">CGCGAATRGCTCATTACAACAGC</td>
<td id="768FF95CFF882BC9FAC222C58D99F924" box="[1337,1430,1676,1696]" gridcol="3" gridrow="1" pageId="3" pageNumber="4">[27]</td>
</tr>
<tr id="355E9020FF882BC9FF6C22E58D99F944" box="[151,1430,1708,1728]" gridrow="2" pageId="3" pageNumber="4" rowspan-0="1" rowspan-3="1">
<td id="768FF95CFF882BC9FDFB22E58A66F944" box="[512,617,1708,1728]" gridcol="1" gridrow="2" pageId="3" pageNumber="4">Nem_18S_R</td>
<td id="768FF95CFF882BC9FCC622E58C7DF944" box="[829,1138,1708,1728]" gridcol="2" gridrow="2" pageId="3" pageNumber="4">GGGCGGTATCTGATCGCC</td>
</tr>
<tr id="355E9020FF882BC9FF6C22858D99F964" box="[151,1430,1740,1760]" gridrow="3" pageId="3" pageNumber="4">
<th id="768FF95CFF882BC9FF6C22858923F964" box="[151,300,1740,1760]" gridcol="0" gridrow="3" pageId="3" pageNumber="4">28S rDNA</th>
<td id="768FF95CFF882BC9FDFB22858A66F964" box="[512,617,1740,1760]" gridcol="1" gridrow="3" pageId="3" pageNumber="4">D2A</td>
<td id="768FF95CFF882BC9FCC622858C7DF964" box="[829,1138,1740,1760]" gridcol="2" gridrow="3" pageId="3" pageNumber="4">ACAAGTACCGTGAGGGAAAGT</td>
<td id="768FF95CFF882BC9FAC222858D99F964" box="[1337,1430,1740,1760]" gridcol="3" gridrow="3" pageId="3" pageNumber="4">
[
<bibRefCitation id="EFFFEF93FF88D435FAC522858D5CF964" author="Ribosomal DNA" box="[1342,1363,1740,1760]" pageId="3" pageNumber="4" pagination="221 - 36" refId="ref12794" refString="28. Nadler SA, Hudspeth DS. Ribosomal DNA and phylogeny of the Ascaridoidea (Nemata: Secernentea): implications for morphological evolution and classification. Mol Phylogenet Evol. 1998; 10: 221 - 36." type="journal article" year="1998">28</bibRefCitation>
, 
<bibRefCitation id="EFFFEF93FF88D435FAA722858D7EF964" author="Pereira FB &amp; Tavares LE &amp; Scholz T &amp; Luque JL" box="[1372,1393,1740,1760]" pageId="3" pageNumber="4" pagination="49 - 61" refId="ref12834" refString="29. Pereira FB, Tavares LE, Scholz T, Luque JL. A morphological and molecular study of two species of Raphidascaroides Yamaguti, 1941 (Nematoda: Anisakidae), parasites of doradid catfish (Siluriformes) in South America, with a description of R. moraveci n. sp. Syst Parasitol. 2015; 91: 49 - 61." type="journal article" year="2015">29</bibRefCitation>
]
</td>
</tr>
<tr id="355E9020FF882BC9FF6C22A58D99F884" box="[151,1430,1772,1792]" gridrow="4" pageId="3" pageNumber="4" rowspan-0="1" rowspan-3="1">
<td id="768FF95CFF882BC9FDFB22A58A66F884" box="[512,617,1772,1792]" gridcol="1" gridrow="4" pageId="3" pageNumber="4">D3B</td>
<td id="768FF95CFF882BC9FCC622A58C7DF884" box="[829,1138,1772,1792]" gridcol="2" gridrow="4" pageId="3" pageNumber="4">TGCGAAGGAACCAGCTACTA</td>
</tr>
<tr id="355E9020FF882BC9FF6C23448D99F8A5" box="[151,1430,1805,1825]" gridrow="5" pageId="3" pageNumber="4" rowspan-0="1" rowspan-3="1">
<td id="768FF95CFF882BC9FDFB23448A66F8A5" box="[512,617,1805,1825]" gridcol="1" gridrow="5" pageId="3" pageNumber="4">28SF</td>
<td id="768FF95CFF882BC9FCC623448C7DF8A5" box="[829,1138,1805,1825]" gridcol="2" gridrow="5" pageId="3" pageNumber="4">AGCGGAGGAAAAGAAACTAA</td>
</tr>
<tr id="355E9020FF882BC9FF6C23648D99F8C5" box="[151,1430,1837,1857]" gridrow="6" pageId="3" pageNumber="4" rowspan-0="1" rowspan-3="1">
<td id="768FF95CFF882BC9FDFB23648A66F8C5" box="[512,617,1837,1857]" gridcol="1" gridrow="6" pageId="3" pageNumber="4">28SR</td>
<td id="768FF95CFF882BC9FCC623648C7DF8C5" box="[829,1138,1837,1857]" gridcol="2" gridrow="6" pageId="3" pageNumber="4">ATCCGTGTTTCAAGACGGG</td>
</tr>
<tr id="355E9020FF882BC9FF6C23048D99F8E5" box="[151,1430,1869,1889]" gridrow="7" pageId="3" pageNumber="4">
<th id="768FF95CFF882BC9FF6C23048923F8E5" box="[151,300,1869,1889]" gridcol="0" gridrow="7" pageId="3" pageNumber="4">ITS region</th>
<td id="768FF95CFF882BC9FDFB23048A66F8E5" box="[512,617,1869,1889]" gridcol="1" gridrow="7" pageId="3" pageNumber="4">NC5</td>
<td id="768FF95CFF882BC9FCC623048C7DF8E5" box="[829,1138,1869,1889]" gridcol="2" gridrow="7" pageId="3" pageNumber="4">GTAGGTGAACCTGCGGAAGGATCATT</td>
<td id="768FF95CFF882BC9FAC223048D99F8E5" box="[1337,1430,1869,1889]" gridcol="3" gridrow="7" pageId="3" pageNumber="4">[30]</td>
</tr>
<tr id="355E9020FF882BC9FF6C23278D99F806" box="[151,1430,1902,1922]" gridrow="8" pageId="3" pageNumber="4" rowspan-0="1" rowspan-3="1">
<td id="768FF95CFF882BC9FDFB23278A66F806" box="[512,617,1902,1922]" gridcol="1" gridrow="8" pageId="3" pageNumber="4">NC2</td>
<td id="768FF95CFF882BC9FCC623278C7DF806" box="[829,1138,1902,1922]" gridcol="2" gridrow="8" pageId="3" pageNumber="4">TTAGTTTCTTTTCCTCCGCT</td>
</tr>
</table>
</paragraph>
<caption id="DF11C2EAFF8FD432FF6C24A78A20FEA5" ID-Table-UUID="DF11C2EAFF8FD432FF6C24A78A20FEA5" httpUri="http://table.plazi.org/id/DF11C2EAFF8FD432FF6C24A78A20FEA5" pageId="4" pageNumber="5" startId="4.[151,209,238,260]" targetBox="[151,773,312,1404]" targetIsTable="true" targetPageId="4" targetType="table">
<paragraph id="8BD19262FF8FD432FF6C24A78A20FEA5" blockId="4.[151,774,238,289]" pageId="4" pageNumber="5">
<emphasis id="B91A4E70FF8FD432FF6C24A788ECFE80" bold="true" box="[151,227,238,260]" pageId="4" pageNumber="5">Table 2</emphasis>
List of GenBank accession numbers for sequence data used in phylogenetic analyses in this study
</paragraph>
</caption>
<paragraph id="8BD19262FF8FD432FF6C25718AB7FAF8" pageId="4" pageNumber="5">
<table id="F96E60C2FF8F2BC9FF6C25718B0AFAF8" box="[151,773,312,1404]" gridcols="3" gridrows="33" pageId="4" pageNumber="5">
<tr id="355E9020FF8F2BC9FF6C25718B0AFEE0" box="[151,773,312,356]" gridrow="0" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C25718975FEE0" box="[151,378,312,356]" gridcol="0" gridrow="0" pageId="4" pageNumber="5">Species</th>
<th id="768FF95CFF8F2BC9FE3225718A68FEE0" box="[457,615,312,356]" gridcol="1" gridrow="0" pageId="4" pageNumber="5">Accession number (18S rDNA)</th>
<th id="768FF95CFF8F2BC9FD6C25718B0AFEE0" box="[663,773,312,356]" gridcol="2" gridrow="0" pageId="4" pageNumber="5">References</th>
</tr>
<tr id="355E9020FF8F2BC9FF6C25C98B0AFE10" box="[151,773,384,404]" gridrow="1" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C25C98975FE10" box="[151,378,384,404]" gridcol="0" gridrow="1" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C25C98914FE10" authority="LC" authorityName="LC" box="[151,283,384,404]" class="Enoplea" family="Capillariidae" genus="Capillaria" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="anatis">
<emphasis id="B91A4E70FF8FD432FF6C25C98914FE10" box="[151,283,384,404]" italics="true" pageId="4" pageNumber="5">Capillaria anatis</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE3225C98A68FE10" box="[457,615,384,404]" gridcol="1" gridrow="1" pageId="4" pageNumber="5">LC052335</td>
<td id="768FF95CFF8F2BC9FD6C25C98B0AFE10" box="[663,773,384,404]" gridcol="2" gridrow="1" pageId="4" pageNumber="5">[77]</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C25E98B0AFE30" box="[151,773,416,436]" gridrow="2" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C25E98975FE30" box="[151,378,416,436]" gridcol="0" gridrow="2" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C25E98927FE30" authority="LC" authorityName="LC" box="[151,296,416,436]" class="Enoplea" family="Capillariidae" genus="Capillaria" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="bursata">
<emphasis id="B91A4E70FF8FD432FF6C25E98927FE30" box="[151,296,416,436]" italics="true" pageId="4" pageNumber="5">Capillaria bursata</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE3225E98A68FE30" box="[457,615,416,436]" gridcol="1" gridrow="2" pageId="4" pageNumber="5">LC425006</td>
<td id="768FF95CFF8F2BC9FD6C25E98B0AFE30" box="[663,773,416,436]" gridcol="2" gridrow="2" pageId="4" pageNumber="5">
[
<bibRefCitation id="EFFFEF93FF8FD432FD6625E98ABDFE30" author="Sakaguchi S &amp; Yunus M &amp; Sugi S &amp; Sato H." box="[669,690,416,436]" pageId="4" pageNumber="5" pagination="957 - 72" refId="ref14966" refString="78. Sakaguchi S, Yunus M, Sugi S, Sato H. Integrated taxonomic approaches to seven species of capillariid nematodes (Nematoda: Trichocephalida: Trichinelloidea) in poultry from Japan and Indonesia, with special reference to their 18 S rDNA phylogenetic relationships. Parasitol Res. 2020; 119: 957 - 72." type="journal article" year="2020">78</bibRefCitation>
]
</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C25888B0AFE51" box="[151,773,449,469]" gridrow="3" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C25888975FE51" box="[151,378,449,469]" gridcol="0" gridrow="3" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C25888920FE51" authority="LC" authorityName="LC" box="[151,303,449,469]" class="Enoplea" family="Capillariidae" genus="Capillaria" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="madseni">
<emphasis id="B91A4E70FF8FD432FF6C25888920FE51" box="[151,303,449,469]" italics="true" pageId="4" pageNumber="5">Capillaria madseni</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE3225888A68FE51" box="[457,615,449,469]" gridcol="1" gridrow="3" pageId="4" pageNumber="5">LC052348</td>
<td id="768FF95CFF8F2BC9FD6C25888B0AFE51" box="[663,773,449,469]" gridcol="2" gridrow="3" pageId="4" pageNumber="5">[77]</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C25A88B0AFE71" box="[151,773,481,501]" gridrow="4" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C25A88975FE71" box="[151,378,481,501]" gridcol="0" gridrow="4" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C25A88953FE71" authority="LC" authorityName="LC" box="[151,348,481,501]" class="Enoplea" family="Capillariidae" genus="Capillaria" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="pudendotecta">
<emphasis id="B91A4E70FF8FD432FF6C25A88953FE71" box="[151,348,481,501]" italics="true" pageId="4" pageNumber="5">Capillaria pudendotecta</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE3225A88A68FE71" box="[457,615,481,501]" gridcol="1" gridrow="4" pageId="4" pageNumber="5">LC052343</td>
<td id="768FF95CFF8F2BC9FD6C25A88B0AFE71" box="[663,773,481,501]" gridcol="2" gridrow="4" pageId="4" pageNumber="5">[77]</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C26488B0AFD91" box="[151,773,513,533]" gridrow="5" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C26488975FD91" box="[151,378,513,533]" gridcol="0" gridrow="5" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C26488939FD91" authority="LC" authorityName="LC" box="[151,310,513,533]" class="Enoplea" family="Capillariidae" genus="Capillaria" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="spinulosa">
<emphasis id="B91A4E70FF8FD432FF6C26488939FD91" box="[151,310,513,533]" italics="true" pageId="4" pageNumber="5">Capillaria spinulosa</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE3226488A68FD91" box="[457,615,513,533]" gridcol="1" gridrow="5" pageId="4" pageNumber="5">LC424999</td>
<td id="768FF95CFF8F2BC9FD6C26488B0AFD91" box="[663,773,513,533]" gridcol="2" gridrow="5" pageId="4" pageNumber="5">
[
<bibRefCitation id="EFFFEF93FF8FD432FD6626488ABDFD91" author="Sakaguchi S &amp; Yunus M &amp; Sugi S &amp; Sato H." box="[669,690,513,533]" pageId="4" pageNumber="5" pagination="957 - 72" refId="ref14966" refString="78. Sakaguchi S, Yunus M, Sugi S, Sato H. Integrated taxonomic approaches to seven species of capillariid nematodes (Nematoda: Trichocephalida: Trichinelloidea) in poultry from Japan and Indonesia, with special reference to their 18 S rDNA phylogenetic relationships. Parasitol Res. 2020; 119: 957 - 72." type="journal article" year="2020">78</bibRefCitation>
]
</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C266B8B0AFDB2" box="[151,773,546,566]" gridrow="6" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C266B8975FDB2" box="[151,378,546,566]" gridcol="0" gridrow="6" pageId="4" pageNumber="5">
<emphasis id="B91A4E70FF8FD432FF6C266B8908FDB2" box="[151,263,546,566]" italics="true" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C266B88EAFDB2" authorityName="Zeder" authorityYear="1800" box="[151,229,546,566]" class="Enoplea" family="Capillariidae" genus="Capillaria" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="genus">Capillaria</taxonomicName>
suis
</emphasis>
</th>
<td id="768FF95CFF8F2BC9FE32266B8A68FDB2" box="[457,615,546,566]" gridcol="1" gridrow="6" pageId="4" pageNumber="5">LC052376</td>
<td id="768FF95CFF8F2BC9FD6C266B8B0AFDB2" box="[663,773,546,566]" gridcol="2" gridrow="6" pageId="4" pageNumber="5">[77]</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C260B8B0AFDD2" box="[151,773,578,598]" gridrow="7" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C260B8975FDD2" box="[151,378,578,598]" gridcol="0" gridrow="7" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C260B894EFDD2" authority="EU" authorityName="EU" box="[151,321,578,598]" class="Enoplea" family="Capillariidae" genus="Capillaria" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="tenuissima">
<emphasis id="B91A4E70FF8FD432FF6C260B894EFDD2" box="[151,321,578,598]" italics="true" pageId="4" pageNumber="5">Capillaria tenuissima</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE32260B8A68FDD2" box="[457,615,578,598]" gridcol="1" gridrow="7" pageId="4" pageNumber="5">EU004822</td>
<td id="768FF95CFF8F2BC9FD6C260B8B0AFDD2" box="[663,773,578,598]" gridcol="2" gridrow="7" pageId="4" pageNumber="5">
[
<bibRefCitation id="EFFFEF93FF8FD432FD66260B8ABDFDD2" author="Honisch M &amp; Krone O." box="[669,690,578,598]" pageId="4" pageNumber="5" pagination="129 - 33" refId="ref15025" refString="79. Honisch M, Krone O. Phylogenetic relationships of Spiruromorpha from birds of prey based on 18 S rDNA. J Helminthol. 2008; 82: 129 - 33." type="journal article" year="2008">79</bibRefCitation>
]
</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C262B8B0AFDF2" box="[151,773,610,630]" gridrow="8" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C262B8975FDF2" box="[151,378,610,630]" gridcol="0" gridrow="8" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C262B8950FDF2" authority="ON" authorityName="ON" box="[151,351,610,630]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" isUncertain="true" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="huffmani">
<emphasis id="B91A4E70FF8FD432FF6C262B88F6FDF2" box="[151,249,610,630]" italics="true" pageId="4" pageNumber="5">Huffmanela</emphasis>
cf. 
<emphasis id="B91A4E70FF8FD432FEE8262B8950FDF2" box="[275,351,610,630]" italics="true" pageId="4" pageNumber="5">huffmani</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE32262B8A68FDF2" box="[457,615,610,630]" gridcol="1" gridrow="8" pageId="4" pageNumber="5">ON838247</td>
<td id="768FF95CFF8F2BC9FD6C262B8B0AFDF2" box="[663,773,610,630]" gridcol="2" gridrow="8" pageId="4" pageNumber="5">[5]</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C26CB8B0AFD12" box="[151,773,642,662]" gridrow="9" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C26CB8975FD12" box="[151,378,642,662]" gridcol="0" gridrow="9" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C26CB8950FD12" authority="ON" authorityName="ON" box="[151,351,642,662]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" isUncertain="true" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="huffmani">
<emphasis id="B91A4E70FF8FD432FF6C26CB88F6FD12" box="[151,249,642,662]" italics="true" pageId="4" pageNumber="5">Huffmanela</emphasis>
cf. 
<emphasis id="B91A4E70FF8FD432FEE826CB8950FD12" box="[275,351,642,662]" italics="true" pageId="4" pageNumber="5">huffmani</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE3226CB8A68FD12" box="[457,615,642,662]" gridcol="1" gridrow="9" pageId="4" pageNumber="5">ON838248</td>
<td id="768FF95CFF8F2BC9FD6C26CB8B0AFD12" box="[663,773,642,662]" gridcol="2" gridrow="9" pageId="4" pageNumber="5">[5]</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C26EA8B0AFD33" box="[151,773,675,695]" gridrow="10" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C26EA8975FD33" box="[151,378,675,695]" gridcol="0" gridrow="10" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C26EA8946FD33" authority="ON" authorityName="ON" box="[151,329,675,695]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="huffmani">
<emphasis id="B91A4E70FF8FD432FF6C26EA8946FD33" box="[151,329,675,695]" italics="true" pageId="4" pageNumber="5">Huffmanela huffmani</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE3226EA8A68FD33" box="[457,615,675,695]" gridcol="1" gridrow="10" pageId="4" pageNumber="5">ON838249</td>
<td id="768FF95CFF8F2BC9FD6C26EA8B0AFD33" box="[663,773,675,695]" gridcol="2" gridrow="10" pageId="4" pageNumber="5">[5]</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C268A8B0AFD53" box="[151,773,707,727]" gridrow="11" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C268A8975FD53" box="[151,378,707,727]" gridcol="0" gridrow="11" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C268A8946FD53" authority="ON" authorityName="ON" box="[151,329,707,727]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="huffmani">
<emphasis id="B91A4E70FF8FD432FF6C268A8946FD53" box="[151,329,707,727]" italics="true" pageId="4" pageNumber="5">Huffmanela huffmani</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE32268A8A68FD53" box="[457,615,707,727]" gridcol="1" gridrow="11" pageId="4" pageNumber="5">ON838251</td>
<td id="768FF95CFF8F2BC9FD6C268A8B0AFD53" box="[663,773,707,727]" gridcol="2" gridrow="11" pageId="4" pageNumber="5">[5]</td>
</tr>
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<emphasis id="B91A4E70FF8FD432FF6C26AA8956FD73" box="[151,345,739,759]" italics="true" pageId="4" pageNumber="5">Huffmanela markgracei</emphasis>
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<td id="768FF95CFF8F2BC9FD6C26AA8B0AFD73" box="[663,773,739,759]" gridcol="2" gridrow="12" pageId="4" pageNumber="5">[5]</td>
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<emphasis id="B91A4E70FF8FD432FF6C274A8939FC93" box="[151,310,771,791]" italics="true" pageId="4" pageNumber="5">Huffmanela persica</emphasis>
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<td id="768FF95CFF8F2BC9FD6C274A8B0AFC93" box="[663,773,771,791]" gridcol="2" gridrow="13" pageId="4" pageNumber="5">This study</td>
</tr>
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<emphasis id="B91A4E70FF8FD432FF6C276D88F6FCBC" box="[151,249,804,824]" italics="true" pageId="4" pageNumber="5">Huffmanela</emphasis>
sp.
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</th>
<td id="768FF95CFF8F2BC9FE32276D8A68FCBC" box="[457,615,804,824]" gridcol="1" gridrow="14" pageId="4" pageNumber="5">ON838246</td>
<td id="768FF95CFF8F2BC9FD6C276D8B0AFCBC" box="[663,773,804,824]" gridcol="2" gridrow="14" pageId="4" pageNumber="5">[5]</td>
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<emphasis id="B91A4E70FF8FD432FF6C270D8975FCDC" box="[151,378,836,856]" italics="true" pageId="4" pageNumber="5">Pseudocapillaria tomentosa</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE32270D8A68FCDC" box="[457,615,836,856]" gridcol="1" gridrow="15" pageId="4" pageNumber="5">KU987805</td>
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[
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<taxonomicName id="4C6EE9E1FF8FD432FF6C272D892EFCFC" authority="AY" authorityName="AY" box="[151,289,868,888]" class="Enoplea" family="Trichinellidae" genus="Trichinella" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="britovi">
<emphasis id="B91A4E70FF8FD432FF6C272D892EFCFC" box="[151,289,868,888]" italics="true" pageId="4" pageNumber="5">Trichinella britovi</emphasis>
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<taxonomicName id="4C6EE9E1FF8FD432FF6C27CD8923FC1C" authority="AY" authorityName="AY" box="[151,300,900,920]" class="Enoplea" family="Trichinellidae" genus="Trichinella" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="murrelli">
<emphasis id="B91A4E70FF8FD432FF6C27CD8923FC1C" box="[151,300,900,920]" italics="true" pageId="4" pageNumber="5">Trichinella murrelli</emphasis>
</taxonomicName>
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<td id="768FF95CFF8F2BC9FE3227CD8A68FC1C" box="[457,615,900,920]" gridcol="1" gridrow="17" pageId="4" pageNumber="5">AY851259</td>
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<taxonomicName id="4C6EE9E1FF8FD432FF6C27EC892DFC3D" authority="AY" authorityName="AY" box="[151,290,933,953]" class="Enoplea" family="Trichinellidae" genus="Trichinella" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="nativa">
<emphasis id="B91A4E70FF8FD432FF6C27EC892DFC3D" box="[151,290,933,953]" italics="true" pageId="4" pageNumber="5">Trichinella nativa</emphasis>
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<td id="768FF95CFF8F2BC9FD6C27EC8B0AFC3D" box="[663,773,933,953]" gridcol="2" gridrow="18" pageId="4" pageNumber="5">
[
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<th id="768FF95CFF8F2BC9FF6C278C8975FC5D" box="[151,378,965,985]" gridcol="0" gridrow="19" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C278C8927FC5D" authority="AY" authorityName="AY" box="[151,296,965,985]" class="Enoplea" family="Trichinellidae" genus="Trichinella" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="nelsoni">
<emphasis id="B91A4E70FF8FD432FF6C278C8927FC5D" box="[151,296,965,985]" italics="true" pageId="4" pageNumber="5">Trichinella nelsoni</emphasis>
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<td id="768FF95CFF8F2BC9FE32278C8A68FC5D" box="[457,615,965,985]" gridcol="1" gridrow="19" pageId="4" pageNumber="5">AY851261</td>
<td id="768FF95CFF8F2BC9FD6C278C8B0AFC5D" box="[663,773,965,985]" gridcol="2" gridrow="19" pageId="4" pageNumber="5">
[
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<th id="768FF95CFF8F2BC9FF6C27AC8975FC7D" box="[151,378,997,1017]" gridcol="0" gridrow="20" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C27AC8922FC7D" authority="AY" authorityName="AY" box="[151,301,997,1017]" class="Enoplea" family="Trichinellidae" genus="Trichinella" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="papuae">
<emphasis id="B91A4E70FF8FD432FF6C27AC8922FC7D" box="[151,301,997,1017]" italics="true" pageId="4" pageNumber="5">Trichinella papuae</emphasis>
</taxonomicName>
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<td id="768FF95CFF8F2BC9FE3227AC8A68FC7D" box="[457,615,997,1017]" gridcol="1" gridrow="20" pageId="4" pageNumber="5">AY851263</td>
<td id="768FF95CFF8F2BC9FD6C27AC8B0AFC7D" box="[663,773,997,1017]" gridcol="2" gridrow="20" pageId="4" pageNumber="5">
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<th id="768FF95CFF8F2BC9FF6C204C8975FB9D" box="[151,378,1029,1049]" gridcol="0" gridrow="21" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C204C896FFB9D" authority="MF" authorityName="MF" box="[151,352,1029,1049]" class="Enoplea" family="Trichinellidae" genus="Trichinella" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="patagoniensis">
<emphasis id="B91A4E70FF8FD432FF6C204C896FFB9D" box="[151,352,1029,1049]" italics="true" pageId="4" pageNumber="5">Trichinella patagoniensis</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE32204C8A68FB9D" box="[457,615,1029,1049]" gridcol="1" gridrow="21" pageId="4" pageNumber="5">MF628272</td>
<td id="768FF95CFF8F2BC9FD6C204C8B0AFB9D" box="[663,773,1029,1049]" gridcol="2" gridrow="21" pageId="4" pageNumber="5">
[
<bibRefCitation id="EFFFEF93FF8FD432FD66204C8ABDFB9D" author="Krivokapich SJ" box="[669,690,1029,1049]" pageId="4" pageNumber="5" refId="ref15162" refString="82. Krivokapich SJ. Detection and molecular, biological, morphological and evolutionary study of the first autochthonous Trichinella species found in the Neotropical region. PhD thesis (In Argentinian). 2017; 165 p. https: // hdl. handle. net / 20.500.12110 / tesis _ n 6276 _ Krivokapich." type="book" year="2017">82</bibRefCitation>
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<tr id="355E9020FF8F2BC9FF6C206F8B0AFBBE" box="[151,773,1062,1082]" gridrow="22" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C206F8975FBBE" box="[151,378,1062,1082]" gridcol="0" gridrow="22" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C206F892AFBBE" box="[151,293,1062,1082]" class="Enoplea" family="Trichinellidae" genus="Trichinella" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="spiralis">
<emphasis id="B91A4E70FF8FD432FF6C206F892AFBBE" box="[151,293,1062,1082]" italics="true" pageId="4" pageNumber="5">Trichinella spiralis</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE32206F8A68FBBE" box="[457,615,1062,1082]" gridcol="1" gridrow="22" pageId="4" pageNumber="5">U60231</td>
<td id="768FF95CFF8F2BC9FD6C206F8B0AFBBE" box="[663,773,1062,1082]" gridcol="2" gridrow="22" pageId="4" pageNumber="5">[83]</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C200F8B0AFBDE" box="[151,773,1094,1114]" gridrow="23" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C200F8975FBDE" box="[151,378,1094,1114]" gridcol="0" gridrow="23" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C200F896DFBDE" authority="AY" authorityName="AY" box="[151,354,1094,1114]" class="Enoplea" family="Trichinellidae" genus="Trichinella" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="zimbabwensis">
<emphasis id="B91A4E70FF8FD432FF6C200F896DFBDE" box="[151,354,1094,1114]" italics="true" pageId="4" pageNumber="5">Trichinella zimbabwensis</emphasis>
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</th>
<td id="768FF95CFF8F2BC9FE32200F8A68FBDE" box="[457,615,1094,1114]" gridcol="1" gridrow="23" pageId="4" pageNumber="5">AY851264</td>
<td id="768FF95CFF8F2BC9FD6C200F8B0AFBDE" box="[663,773,1094,1114]" gridcol="2" gridrow="23" pageId="4" pageNumber="5">
[
<bibRefCitation id="EFFFEF93FF8FD432FD66200F8ABDFBDE" author="Zarlenga DS &amp; Rosenthal BM &amp; La Rosa G &amp; Pozio E &amp; Hoberg EP" box="[669,690,1094,1114]" pageId="4" pageNumber="5" pagination="7354 - 9" refId="ref15112" refString="81. Zarlenga DS, Rosenthal BM, La Rosa G, Pozio E, Hoberg EP. Post-Miocene expansion, colonization, and host switching drove speciation among extant nematodes of the archaic genus Trichinella. Proc Natl Acad Sci. 2006; 103: 7354 - 9." type="journal article" year="2006">81</bibRefCitation>
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<tr id="355E9020FF8F2BC9FF6C202F8B0AFBFE" box="[151,773,1126,1146]" gridrow="24" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C202F8975FBFE" box="[151,378,1126,1146]" gridcol="0" gridrow="24" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C202F8975FBFE" authority="LC" authorityName="LC" box="[151,378,1126,1146]" family="Trichosomoididae" genus="Trichosomoides" higherTaxonomySource="GBIF" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="crassicauda">
<emphasis id="B91A4E70FF8FD432FF6C202F8975FBFE" box="[151,378,1126,1146]" italics="true" pageId="4" pageNumber="5">Trichosomoides crassicauda</emphasis>
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</th>
<td id="768FF95CFF8F2BC9FE32202F8A68FBFE" box="[457,615,1126,1146]" gridcol="1" gridrow="24" pageId="4" pageNumber="5">LC425007</td>
<td id="768FF95CFF8F2BC9FD6C202F8B0AFBFE" box="[663,773,1126,1146]" gridcol="2" gridrow="24" pageId="4" pageNumber="5">Unpublished</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C20CF8B0AFB1E" box="[151,773,1158,1178]" gridrow="25" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C20CF8975FB1E" box="[151,378,1158,1178]" gridcol="0" gridrow="25" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C20CF8927FB1E" authority="HF" authorityName="HF" box="[151,296,1158,1178]" class="Enoplea" family="Trichuridae" genus="Trichuris" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="arvicolae">
<emphasis id="B91A4E70FF8FD432FF6C20CF8927FB1E" box="[151,296,1158,1178]" italics="true" pageId="4" pageNumber="5">Trichuris arvicolae</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE3220CF8A68FB1E" box="[457,615,1158,1178]" gridcol="1" gridrow="25" pageId="4" pageNumber="5">HF586908</td>
<td id="768FF95CFF8F2BC9FD6C20CF8B0AFB1E" box="[663,773,1158,1178]" gridcol="2" gridrow="25" pageId="4" pageNumber="5">[84]</td>
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<tr id="355E9020FF8F2BC9FF6C20EE8B0AFB3F" box="[151,773,1191,1211]" gridrow="26" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C20EE8975FB3F" box="[151,378,1191,1211]" gridcol="0" gridrow="26" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C20EE8912FB3F" authority="HF" authorityName="HF" box="[151,285,1191,1211]" class="Enoplea" family="Trichuridae" genus="Trichuris" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="discolor">
<emphasis id="B91A4E70FF8FD432FF6C20EE8912FB3F" box="[151,285,1191,1211]" italics="true" pageId="4" pageNumber="5">Trichuris discolor</emphasis>
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</th>
<td id="768FF95CFF8F2BC9FE3220EE8A68FB3F" box="[457,615,1191,1211]" gridcol="1" gridrow="26" pageId="4" pageNumber="5">HF586910</td>
<td id="768FF95CFF8F2BC9FD6C20EE8B0AFB3F" box="[663,773,1191,1211]" gridcol="2" gridrow="26" pageId="4" pageNumber="5">[84]</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C208E8B0AFB5F" box="[151,773,1223,1243]" gridrow="27" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C208E8975FB5F" box="[151,378,1223,1243]" gridcol="0" gridrow="27" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C208E8903FB5F" authority="AF" authorityName="AF" box="[151,268,1223,1243]" class="Enoplea" family="Trichuridae" genus="Trichuris" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="muris">
<emphasis id="B91A4E70FF8FD432FF6C208E8903FB5F" box="[151,268,1223,1243]" italics="true" pageId="4" pageNumber="5">Trichuris muris</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE32208E8A68FB5F" box="[457,615,1223,1243]" gridcol="1" gridrow="27" pageId="4" pageNumber="5">AF036637</td>
<td id="768FF95CFF8F2BC9FD6C208E8B0AFB5F" box="[663,773,1223,1243]" gridcol="2" gridrow="27" pageId="4" pageNumber="5">[85]</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C20AE8B0AFB7F" box="[151,773,1255,1275]" gridrow="28" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C20AE8975FB7F" box="[151,378,1255,1275]" gridcol="0" gridrow="28" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C20AE88F2FB7F" authority="HF" authorityName="HF" box="[151,253,1255,1275]" class="Enoplea" family="Trichuridae" genus="Trichuris" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="ovis">
<emphasis id="B91A4E70FF8FD432FF6C20AE88F2FB7F" box="[151,253,1255,1275]" italics="true" pageId="4" pageNumber="5">Trichuris ovis</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE3220AE8A68FB7F" box="[457,615,1255,1275]" gridcol="1" gridrow="28" pageId="4" pageNumber="5">HF586911</td>
<td id="768FF95CFF8F2BC9FD6C20AE8B0AFB7F" box="[663,773,1255,1275]" gridcol="2" gridrow="28" pageId="4" pageNumber="5">[84]</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C214E8B0AFA9F" box="[151,773,1287,1307]" gridrow="29" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C214E8975FA9F" box="[151,378,1287,1307]" gridcol="0" gridrow="29" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C214E892DFA9F" authority="HF" authorityName="HF" box="[151,290,1287,1307]" class="Enoplea" family="Trichuridae" genus="Trichuris" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="skrjabini">
<emphasis id="B91A4E70FF8FD432FF6C214E892DFA9F" box="[151,290,1287,1307]" italics="true" pageId="4" pageNumber="5">Trichuris skrjabini</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE32214E8A68FA9F" box="[457,615,1287,1307]" gridcol="1" gridrow="29" pageId="4" pageNumber="5">HF586912</td>
<td id="768FF95CFF8F2BC9FD6C214E8B0AFA9F" box="[663,773,1287,1307]" gridcol="2" gridrow="29" pageId="4" pageNumber="5">[84]</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C21618B0AFAB8" box="[151,773,1320,1340]" gridrow="30" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C21618975FAB8" box="[151,378,1320,1340]" gridcol="0" gridrow="30" pageId="4" pageNumber="5">
<emphasis id="B91A4E70FF8FD432FF6C216188F3FAB8" box="[151,252,1320,1340]" italics="true" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C216188D5FAB8" authorityName="Roederer" authorityYear="1761" box="[151,218,1320,1340]" class="Enoplea" family="Trichuridae" genus="Trichuris" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="genus">Trichuris</taxonomicName>
suis
</emphasis>
</th>
<td id="768FF95CFF8F2BC9FE3221618A68FAB8" box="[457,615,1320,1340]" gridcol="1" gridrow="30" pageId="4" pageNumber="5">HF586905</td>
<td id="768FF95CFF8F2BC9FD6C21618B0AFAB8" box="[663,773,1320,1340]" gridcol="2" gridrow="30" pageId="4" pageNumber="5">[84]</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C21018B0AFAD8" box="[151,773,1352,1372]" gridrow="31" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C21018975FAD8" box="[151,378,1352,1372]" gridcol="0" gridrow="31" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C2101892DFAD8" authority="LC" authorityName="LC" box="[151,290,1352,1372]" class="Enoplea" family="Trichuridae" genus="Trichuris" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="trichiura">
<emphasis id="B91A4E70FF8FD432FF6C2101892DFAD8" box="[151,290,1352,1372]" italics="true" pageId="4" pageNumber="5">Trichuris trichiura</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE3221018A68FAD8" box="[457,615,1352,1372]" gridcol="1" gridrow="31" pageId="4" pageNumber="5">LC596914</td>
<td id="768FF95CFF8F2BC9FD6C21018B0AFAD8" box="[663,773,1352,1372]" gridcol="2" gridrow="31" pageId="4" pageNumber="5">Unpublished</td>
</tr>
<tr id="355E9020FF8F2BC9FF6C21218B0AFAF8" box="[151,773,1384,1404]" gridrow="32" pageId="4" pageNumber="5">
<th id="768FF95CFF8F2BC9FF6C21218975FAF8" box="[151,378,1384,1404]" gridcol="0" gridrow="32" pageId="4" pageNumber="5">
<taxonomicName id="4C6EE9E1FF8FD432FF6C21218902FAF8" authority="HF" authorityName="HF" box="[151,269,1384,1404]" class="Enoplea" family="Trichuridae" genus="Trichuris" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="vulpis">
<emphasis id="B91A4E70FF8FD432FF6C21218902FAF8" box="[151,269,1384,1404]" italics="true" pageId="4" pageNumber="5">Trichuris vulpis</emphasis>
</taxonomicName>
</th>
<td id="768FF95CFF8F2BC9FE3221218A68FAF8" box="[457,615,1384,1404]" gridcol="1" gridrow="32" pageId="4" pageNumber="5">HF586909</td>
<td id="768FF95CFF8F2BC9FD6C21218B0AFAF8" box="[663,773,1384,1404]" gridcol="2" gridrow="32" pageId="4" pageNumber="5">[84]</td>
</tr>
</table>
</paragraph>
<caption id="DF11C2EAFF8FD432FCC620008C9BFB79" ID-DOI="http://doi.org/10.5281/zenodo.11552134" ID-Zenodo-Dep="11552134" httpUri="https://zenodo.org/record/11552134/files/figure.png" pageId="4" pageNumber="5" startId="4.[829,860,1097,1117]" targetBox="[840,1411,238,1082]" targetPageId="4" targetType="figure">
<paragraph id="8BD19262FF8FD432FCC620008C9BFB79" blockId="4.[829,1406,1097,1277]" pageId="4" pageNumber="5">
<emphasis id="B91A4E70FF8FD432FCC620008B60FBD9" bold="true" box="[829,879,1097,1117]" pageId="4" pageNumber="5">Fig. 2</emphasis>
Macroscopic and microscopic appearance of fully developed eggs of 
<taxonomicName id="4C6EE9E1FF8FD432FC7B202A8C10FBF3" authority="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh, 2023" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[896,1055,1123,1143]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="persica" status="sp. nov.">
<emphasis id="B91A4E70FF8FD432FC7B202A8C10FBF3" box="[896,1055,1123,1143]" italics="true" pageId="4" pageNumber="5">Huffmanela persica</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A229F30BFF8FD432FBD8202A8C6BFBF3" box="[1059,1124,1123,1143]" pageId="4" pageNumber="5" rank="species">sp. nov.</taxonomicNameLabel>
<emphasis id="B91A4E70FF8FD432FB9C202A8C7AFBF3" bold="true" box="[1127,1141,1123,1143]" pageId="4" pageNumber="5">A</emphasis>
Grossly visible lesions of eggs previously deposited by adult forms of 
<taxonomicName id="4C6EE9E1FF8FD432FB7320378CDAFB16" authority="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh, 2023" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[1160,1237,1150,1170]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="persica" status="sp. nov.">
<emphasis id="B91A4E70FF8FD432FB7320378CDAFB16" box="[1160,1237,1150,1170]" italics="true" pageId="4" pageNumber="5">H.persica</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A229F30BFF8FD432FB2120378D14FB16" box="[1242,1307,1150,1170]" pageId="4" pageNumber="5" rank="species">sp. nov.</taxonomicNameLabel>
in the form of dark spots of various size within the infected tissues (ovary and serosa of stomach) of 
<taxonomicName id="4C6EE9E1FF8FD432FC0D20FA8C90FB43" baseAuthorityName="Forsskal" baseAuthorityYear="1775" box="[1014,1183,1203,1223]" family="Muraenesocidae" genus="Muraenesox" kingdom="Animalia" order="Anguilliformes" pageId="4" pageNumber="5" phylum="Chordata" rank="species" species="cinereus">
<emphasis id="B91A4E70FF8FD432FC0D20FA8C90FB43" box="[1014,1183,1203,1223]" italics="true" pageId="4" pageNumber="5">Muraenesox cinereus</emphasis>
</taxonomicName>
. 
<emphasis id="B91A4E70FF8FD432FB5320FA8CBBFB43" bold="true" box="[1192,1204,1203,1223]" pageId="4" pageNumber="5">B</emphasis>
Wet mount prepared from infected ovary illustrating variously oriented advanced eggs of 
<taxonomicName id="4C6EE9E1FF8FD432FCC620A08B85FB79" authority="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh, 2023" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[829,906,1257,1277]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="persica" status="sp.nov.">
<emphasis id="B91A4E70FF8FD432FCC620A08B85FB79" box="[829,906,1257,1277]" italics="true" pageId="4" pageNumber="5">H.persica</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A229F30BFF8FD432FC7420A08BDFFB79" box="[911,976,1257,1277]" pageId="4" pageNumber="5" rank="species">sp.nov.</taxonomicNameLabel>
within the egg clusters
</paragraph>
</caption>
<paragraph id="8BD19262FF8FD432FF5622118A6AF9F4" blockId="4.[151,775,1528,1936]" box="[173,613,1624,1648]" pageId="4" pageNumber="5">
<emphasis id="B91A4E70FF8FD432FF562211892AF9F4" box="[173,293,1624,1648]" italics="true" pageId="4" pageNumber="5">Prevalence</emphasis>
: 65% (13 infected out of 20).
</paragraph>
</subSubSection>
<subSubSection id="C374C1E9FF8FD432FF5622318A48F934" pageId="4" pageNumber="5" type="etymology">
<paragraph id="8BD19262FF8FD432FF5622318A48F934" blockId="4.[151,775,1528,1936]" pageId="4" pageNumber="5">
<emphasis id="B91A4E70FF8FD432FF562231892EF914" box="[173,289,1656,1680]" italics="true" pageId="4" pageNumber="5">Etymology</emphasis>
: Te specific name 
<taxonomicName id="4C6EE9E1FF8FD432FDFF22308AB0F914" authority="(Persian)" baseAuthorityName="Persian" box="[516,703,1656,1680]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="persica">
<emphasis id="B91A4E70FF8FD432FDFF22308A5CF914" box="[516,595,1657,1680]" italics="true" pageId="4" pageNumber="5">persica</emphasis>
(Persian)
</taxonomicName>
refers to the country of its occurrence (
<collectingCountry id="F379D2F2FF8FD432FDF322D18A35F934" box="[520,570,1688,1712]" name="Iran" pageId="4" pageNumber="5">Iran</collectingCountry>
).
</paragraph>
</subSubSection>
<subSubSection id="C374C1E9FF8FD432FF5622F189DAF8B4" pageId="4" pageNumber="5" type="description">
<paragraph id="8BD19262FF8FD432FF5622F189DAF8B4" blockId="4.[151,775,1528,1936]" pageId="4" pageNumber="5">
<emphasis id="B91A4E70FF8FD432FF5622F189EFF954" box="[173,480,1720,1744]" italics="true" pageId="4" pageNumber="5">Molecular characterization</emphasis>
: Nucleotide sequences of the 18S rDNA (OQ418445), 28S rDNA (OQ428648) and ITS rDNA (OQ428649) of the new species have been deposited in GenBank.
</paragraph>
</subSubSection>
<subSubSection id="C374C1E9FF8FD432FF5623718CC0FA14" pageId="4" pageNumber="5" type="materials_examined">
<paragraph id="8BD19262FF8FD432FF5623718CC0FA14" blockId="4.[151,775,1528,1936]" lastBlockId="4.[813,1436,1363,1424]" pageId="4" pageNumber="5">
<emphasis id="B91A4E70FF8FD432FF562371899EF8CB" box="[173,401,1848,1872]" italics="true" pageId="4" pageNumber="5">Deposited specimens</emphasis>
: 
<materialsCitation id="3B06983FFF8FD432FE5B23718CC4FA14" ID-GBIF-Occurrence="4899303302" country="Czech Republic" county="Ceske Budejovice" location="Helminthological Collection" municipality="Czech Academy of Sciences" pageId="4" pageNumber="5" specimenCount="1" typeStatus="syntype">
<typeStatus id="54D52CC0FF8FD432FE5B237189F6F8D4" box="[416,505,1848,1872]" pageId="4" pageNumber="5" type="syntype">Syntype</typeStatus>
eggs collected from the host (
<taxonomicName id="4C6EE9E1FF8FD432FF2323118957F8EB" baseAuthorityName="Forsskal" baseAuthorityYear="1775" box="[216,344,1880,1903]" family="Muraenesocidae" genus="Muraenesox" kingdom="Animalia" order="Anguilliformes" pageId="4" pageNumber="5" phylum="Chordata" rank="species" species="cinereus">
<emphasis id="B91A4E70FF8FD432FF2323118957F8EB" box="[216,344,1880,1903]" italics="true" pageId="4" pageNumber="5">M. cinereus</emphasis>
</taxonomicName>
) were deposited in the 
<location id="8EB1C4B9FF8FD432FD9A23118933F814" LSID="urn:lsid:plazi:treatment:03C72374FF88D43FFCD620648C22FCE7:8EB1C4B9FF8FD432FD9A23118933F814" country="Czech Republic" county="Ceske Budejovice" municipality="Czech Academy of Sciences" name="Helminthological Collection" pageId="4" pageNumber="5">Helminthological Collection</location>
, 
<location id="8EB1C4B9FF8FD432FEBC23318A5AF814" LSID="urn:lsid:plazi:treatment:03C72374FF88D43FFCD620648C22FCE7:8EB1C4B9FF8FD432FEBC23318A5AF814" box="[327,597,1912,1936]" country="Czech Republic" county="Ceske Budejovice" municipality="Czech Academy of Sciences" name="Institute of Parasitology" pageId="4" pageNumber="5">Institute of Parasitology</location>
, 
<location id="8EB1C4B9FF8FD432FDA423318B09F814" LSID="urn:lsid:plazi:treatment:03C72374FF88D43FFCD620648C22FCE7:8EB1C4B9FF8FD432FDA423318B09F814" box="[607,774,1912,1936]" country="Czech Republic" county="Ceske Budejovice" municipality="Czech Academy of Sciences" name="Biology Centre" pageId="4" pageNumber="5">Biology Centre</location>
of the 
<collectingMunicipality id="6BB50818FF8FD432FC8021118CCFFAF4" box="[891,1216,1368,1392]" pageId="4" pageNumber="5">Czech Academy of Sciences</collectingMunicipality>
, 
<collectingCounty id="62B0EAEEFF8FD432FB35211A8D97FAF4" box="[1230,1432,1363,1392]" pageId="4" pageNumber="5">České Budějovice</collectingCounty>
, 
<collectingCountry id="F379D2F2FF8FD432FCD621318BD4FA14" box="[813,987,1400,1424]" name="Czech Republic" pageId="4" pageNumber="5">Czech Republic</collectingCountry>
(catalog no. N-1277)
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="C374C1E9FF8FD43FFCD621F38C22FCE7" lastPageId="9" lastPageNumber="10" pageId="4" pageNumber="5" type="description">
<paragraph id="8BD19262FF8FD432FCD621F38C3FFA54" blockId="4.[813,1437,1463,1776]" box="[813,1072,1463,1488]" pageId="4" pageNumber="5">
<heading id="D099250EFF8FD432FCD621F38C3FFA54" bold="true" box="[813,1072,1463,1488]" fontSize="9" level="2" pageId="4" pageNumber="5" reason="7">
<emphasis id="B91A4E70FF8FD432FCD621F38C3FFA54" bold="true" box="[813,1072,1463,1488]" pageId="4" pageNumber="5">
<emphasis id="B91A4E70FF8FD432FCD621F38C26FA54" bold="true" box="[813,1065,1466,1488]" italics="true" pageId="4" pageNumber="5">ZooBank registration</emphasis>
:
</emphasis>
</heading>
</paragraph>
<paragraph id="8BD19262FF8FD432FCD621908D78F974" blockId="4.[813,1437,1463,1776]" pageId="4" pageNumber="5">
To comply with the regulations set out in article 8.5 of the amended 2012 version of the International Code of Zoological Nomenclature [
<bibRefCitation id="EFFFEF93FF8FD432FBA522518C77F9B4" author="Bull Zool Nomencl" box="[1118,1144,1560,1584]" pageId="4" pageNumber="5" pagination="161 - 9" refId="ref13180" refString="37. International Commission on Zoological Nomenclature (ICZN). Amendment of articles 8, 9, 10, 21 and 78 of the international code of zoological nomenclature to expand and refine methods of publication. Bull Zool Nomencl. 2012; 69: 161 - 9." type="journal article" year="2012">37</bibRefCitation>
], details of the new species have been submitted to ZooBank. Te Life Science Identifier (LSID) of the article is 
<uri id="FFFF9E60FF8FD432FB2422118D98F914" pageId="4" pageNumber="5">
urn:lsid:zoobank. org:pub: 
<uuid id="FFC8A8B7FF8FD432FC7E22318D98F914" box="[901,1431,1656,1680]" pageId="4" pageNumber="5">84ABB846-5AF2-4008-97F6-573A68DFD1BB</uuid>
</uri>
. Te LSID for the new species name 
<taxonomicName id="4C6EE9E1FF8FD432FB1A22D18B59F954" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="persica">
<emphasis id="B91A4E70FF8FD432FB1A22D18B59F954" italics="true" pageId="4" pageNumber="5">Huffmanela persica</emphasis>
</taxonomicName>
is 
<uri id="FFFF9E60FF8FD432FC5522F18D7CF974" pageId="4" pageNumber="5">
urn:lsid:zoobank.org:act: 
<uri id="FFFF9E60FF8FD432FB2422F18D7CF974" pageId="4" pageNumber="5">
urn:lsid:zoobank. org:act: 
<uuid id="FFC8A8B7FF8FD432FC8322918D7CF974" box="[888,1395,1752,1776]" pageId="4" pageNumber="5">E12D7B01-E298-4BEE-AD69-8C075F84B33E</uuid>
</uri>
</uri>
.
</paragraph>
<paragraph id="8BD19262FF8FD432FCD623538BF5F8B5" blockId="4.[813,1436,1818,1936]" box="[813,1018,1818,1841]" pageId="4" pageNumber="5">
<heading id="D099250EFF8FD432FCD623538BF5F8B5" box="[813,1018,1818,1841]" fontSize="9" level="2" pageId="4" pageNumber="5" reason="5">
<emphasis id="B91A4E70FF8FD432FCD623538BF5F8B5" bold="true" box="[813,1018,1818,1841]" pageId="4" pageNumber="5">Description of eggs</emphasis>
</heading>
</paragraph>
<paragraph id="8BD19262FF8FD433FCD623718A42FDC7" blockId="4.[813,1436,1818,1936]" lastBlockId="5.[151,775,235,580]" lastPageId="5" lastPageNumber="6" pageId="4" pageNumber="5">
Grossly visible eggs of 
<taxonomicName id="4C6EE9E1FF8FD432FBCD23708CA8F8D4" authority="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh, 2023" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[1078,1191,1849,1872]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="persica" status="sp. nov.">
<emphasis id="B91A4E70FF8FD432FBCD23708CA8F8D4" box="[1078,1191,1849,1872]" italics="true" pageId="4" pageNumber="5">H. persica</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A229F30BFF8FD432FB4923708D06F8D4" box="[1202,1289,1849,1872]" pageId="4" pageNumber="5" rank="species">sp. nov.</taxonomicNameLabel>
were mostly observed in the form of black aggregates with different dimensions which were randomly distributed in the infected tissues (
<figureCitation id="13558EE7FF8ED433FEB024A28996FE87" box="[331,409,235,259]" captionStart="Fig" captionStartId="4.[829,860,1097,1117]" captionTargetBox="[840,1411,238,1082]" captionTargetId="figure-450@4.[837,1412,236,1084]" captionTargetPageId="4" captionText="Fig. 2 Macroscopic and microscopic appearance of fully developed eggs of Huffmanela persica sp. nov. A Grossly visible lesions of eggs previously deposited by adult forms of H.persica sp. nov. in the form of dark spots of various size within the infected tissues (ovary and serosa of stomach) of Muraenesox cinereus. B Wet mount prepared from infected ovary illustrating variously oriented advanced eggs of H.persica sp.nov.within the egg clusters" figureDoi="http://doi.org/10.5281/zenodo.11552134" httpUri="https://zenodo.org/record/11552134/files/figure.png" pageId="5" pageNumber="6">Fig. 2B</figureCitation>
). Scalpel scrapings obtained from fresh and fixed egg clusters showed thousands of variously oriented eggs with untinted, brown or black eggshells depending on the stage of development of each egg. Four stages of egg development (from less developed to fully developed eggs) are reported herein based upon morphology and morphometry of eggs as well as embryonic stages of development observed in eggs. Tese include stage I (meiotic stage), stage II (early mitotic embryonated stage), stage III (late embryonated stage) and stage IV (vermiform larvated stage).
</paragraph>
<paragraph id="8BD19262FF8ED433FF6C2624891EFD00" blockId="5.[151,775,621,1123]" box="[151,273,621,644]" pageId="5" pageNumber="6">
<heading id="D099250EFF8ED433FF6C2624891EFD00" bold="true" box="[151,273,621,644]" fontSize="9" level="2" pageId="5" pageNumber="6" reason="7">
<emphasis id="B91A4E70FF8ED433FF6C2624891EFD00" bold="true" box="[151,273,621,644]" italics="true" pageId="5" pageNumber="6">Stage I eggs</emphasis>
</heading>
</paragraph>
<paragraph id="8BD19262FF8ED433FF6C26C28AC1FBE7" blockId="5.[151,775,621,1123]" pageId="5" pageNumber="6">
Stage I eggs (
<figureCitation id="13558EE7FF8ED433FEC726C289CFFD27" box="[316,448,651,675]" captionStart="Fig" captionStartId="5.[167,198,1528,1548]" captionTargetBox="[230,1357,237,1690]" captionTargetId="figure-88@6.[227,1361,236,1693]" captionTargetPageId="6" captionText="Fig. 3 General morphology and surface ornamentation pattern of eggs of Huffmanela persica sp. nov. in various stages of development. A–H Photomicrographs and a–h corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). A, a Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). B, b Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. C, c Two-celled mitotic stage of early embryonic development (embryonated). D, d Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers.E, e Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. F, f Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer.G, g Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). H, h Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. I–T Photomicrographs of less developed (I–L), moderately developed (M–P) and fully developed (Q–T) eggs, where the first set of images (I, M, Q) represents overview of these variously advanced eggs, and the second (J, N, R), third (K, O, S) and fourth (L, P, T) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs" figureDoi="http://doi.org/10.5281/zenodo.12518542" httpUri="https://zenodo.org/record/12518542/files/figure.png" pageId="5" pageNumber="6">Figs. 3A, a</figureCitation>
; 
<figureCitation id="13558EE7FF8ED433FE2926C28A18FD27" box="[466,535,651,675]" captionStart="Fig" captionStartId="7.[167,198,1166,1186]" captionTargetBox="[169,1420,239,1148]" captionTargetId="figure-286@7.[166,1421,236,1153]" captionTargetPageId="7" captionText="Fig. 4 Scanning electron micrographs of poorly developed (A–D) and fully developed (E–I) eggs of Huffmanela persica sp. nov. (separated from infected ovary).Less developed eggs spherical shaped (white arrow shows a shrunken and wrinkled egg) and with no evidently developed polar plugs. Fully developed eggs oblong and containing two plugs at poles. Eggs completely surrounded by a UL bearing uniformly based mammiform mounds adorned with tendril-like vermiform appendage emerging from the apex,occasionally adjoined to that of a neighboring mound (green arrowheads). Note the illusory appearance of serrated-like ridges (occasionally in the form of illusory interconnecting ridges,black arrows) in side views which is caused by overlapping of the mound bases (well observed in less developed eggs, red arrowheads). Outer surface of eggshell with irregular protuberances (yellow arrowheads)" figureDoi="http://doi.org/10.5281/zenodo.11552140" httpUri="https://zenodo.org/record/11552140/files/figure.png" pageId="5" pageNumber="6">4A–D</figureCitation>
; 
<figureCitation id="13558EE7FF8ED433FDD226C28A44FD27" box="[553,587,651,675]" captionStart="Fig" captionStartId="8.[167,198,1521,1541]" captionTargetBox="[290,1304,240,1508]" captionTargetId="figure-87@8.[284,1304,236,1508]" captionTargetPageId="8" captionText="Fig. 5 Photomicrographs from histological sections of infected tissues of a daggertooth pike conger eel. A, B Histological sections of the stomach infected by eggs of Huffmanela persica sp. nov. at various stages of development as well as degenerated encysted metazoans (⁎). C, D Sections of the tunica serosa of the stomach parasitized with completely developed eggs.E Sections of the ovarian lamellae infected by both clusters of immature (➔) and developing (➤) eggs, representing immature and previtellogenic oocytes embedded within a loose fibro-granulomatous infiltration containing histiocytes and eosinophilic granular leukocytes (⁎). F Magnified view of histological section of infected ovary showing a fibro-granulomatous infiltrate surrounding clusters of eggs at different stages of development (mainly highly developed eggs, ➤). G High magnification (100×) view of a fully developed egg of H. persica (cross-sectional view) showing larva in-folded within the eggshell and a protruding polar plug at either end. H Less developed eggs with a nearly central nucleus (⁎) and thin eggshell layer and I fully developed eggs containing twisted larvae of H. persica cut on varying planes of section" figureDoi="http://doi.org/10.5281/zenodo.11552142" httpUri="https://zenodo.org/record/11552142/files/figure.png" pageId="5" pageNumber="6">5H</figureCitation>
) were evidently recently laid eggs in probably meiosis I with a distinctly spherical nucleus mainly located centrally in the granular cytoplasm and no developed or two poorly developed plugs at poles. Eggs in stage I spherical shaped (occasionally shrunken and wrinkled), with light amber rigid eggshell walls and no discernable layering. Egg morphometrics (
<emphasis id="B91A4E70FF8ED433FF0027258906FC07" box="[251,265,876,899]" italics="true" pageId="5" pageNumber="6">n</emphasis>
= 15) recorded in stage I was as follows: total length (with uterine layer, UL) 34.14–44.32 (38.12 ± 2.97); total length (without UL) 30.51–39.51 (34.06 ± 2.76); total width (with UL) 32.65–41.25 (35.50 ± 2.06); total width (without UL) 30.45–37.20 (32.60 ± 1.72); shell thickness (with UL) 3.27–6.07 (4.74 ± 0.81); shell thickness (without UL) 1.54–2.99 (2.47 ± 0.44); nucleus length 8.2–10.71 (9.24 ± 0.87); nucleus width 7.57–10.08 (8.53 ± 0.74).
</paragraph>
<paragraph id="8BD19262FF8ED433FF6C20C48918FB20" blockId="5.[151,775,1165,1476]" box="[151,279,1165,1188]" pageId="5" pageNumber="6">
<heading id="D099250EFF8ED433FF6C20C48918FB20" bold="true" box="[151,279,1165,1188]" fontSize="9" level="2" pageId="5" pageNumber="6" reason="7">
<emphasis id="B91A4E70FF8ED433FF6C20C48918FB20" bold="true" box="[151,279,1165,1188]" italics="true" pageId="5" pageNumber="6">Stage II eggs</emphasis>
</heading>
</paragraph>
<paragraph id="8BD19262FF8ED433FF6C20E28BB8FD47" blockId="5.[151,775,1165,1476]" lastBlockId="5.[813,1436,235,707]" pageId="5" pageNumber="6">
Stage II represents various stages of early embryonic development within the eggshell from single-cell (probably meiosis II) stage to multiple cell mitotic stages (
<figureCitation id="13558EE7FF8ED433FF64214289AAFAA7" box="[159,421,1291,1315]" captionStart="Fig" captionStartId="5.[167,198,1528,1548]" captionTargetBox="[230,1357,237,1690]" captionTargetId="figure-88@6.[227,1361,236,1693]" captionTargetPageId="6" captionText="Fig. 3 General morphology and surface ornamentation pattern of eggs of Huffmanela persica sp. nov. in various stages of development. A–H Photomicrographs and a–h corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). A, a Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). B, b Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. C, c Two-celled mitotic stage of early embryonic development (embryonated). D, d Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers.E, e Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. F, f Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer.G, g Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). H, h Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. I–T Photomicrographs of less developed (I–L), moderately developed (M–P) and fully developed (Q–T) eggs, where the first set of images (I, M, Q) represents overview of these variously advanced eggs, and the second (J, N, R), third (K, O, S) and fourth (L, P, T) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs" figureDoi="http://doi.org/10.5281/zenodo.12518542" httpUri="https://zenodo.org/record/12518542/files/figure.png" pageId="5" pageNumber="6">Fig. 3B, b; C, c; D, d</figureCitation>
). Eggs in the single-cell stage (
<figureCitation id="13558EE7FF8ED433FF642162891BFAC7" box="[159,276,1323,1347]" captionStart="Fig" captionStartId="5.[167,198,1528,1548]" captionTargetBox="[230,1357,237,1690]" captionTargetId="figure-88@6.[227,1361,236,1693]" captionTargetPageId="6" captionText="Fig. 3 General morphology and surface ornamentation pattern of eggs of Huffmanela persica sp. nov. in various stages of development. A–H Photomicrographs and a–h corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). A, a Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). B, b Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. C, c Two-celled mitotic stage of early embryonic development (embryonated). D, d Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers.E, e Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. F, f Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer.G, g Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). H, h Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. I–T Photomicrographs of less developed (I–L), moderately developed (M–P) and fully developed (Q–T) eggs, where the first set of images (I, M, Q) represents overview of these variously advanced eggs, and the second (J, N, R), third (K, O, S) and fourth (L, P, T) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs" figureDoi="http://doi.org/10.5281/zenodo.12518542" httpUri="https://zenodo.org/record/12518542/files/figure.png" pageId="5" pageNumber="6">Fig. 3B, b</figureCitation>
) were distinguished by having a spherical nucleus present in the granular cytoplasm and two newly developed polar plugs. Eggs in the two-cell stage (
<figureCitation id="13558EE7FF8ED433FD48212288ACFA27" captionStart="Fig" captionStartId="5.[167,198,1528,1548]" captionTargetBox="[230,1357,237,1690]" captionTargetId="figure-88@6.[227,1361,236,1693]" captionTargetPageId="6" captionText="Fig. 3 General morphology and surface ornamentation pattern of eggs of Huffmanela persica sp. nov. in various stages of development. A–H Photomicrographs and a–h corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). A, a Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). B, b Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. C, c Two-celled mitotic stage of early embryonic development (embryonated). D, d Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers.E, e Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. F, f Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer.G, g Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). H, h Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. I–T Photomicrographs of less developed (I–L), moderately developed (M–P) and fully developed (Q–T) eggs, where the first set of images (I, M, Q) represents overview of these variously advanced eggs, and the second (J, N, R), third (K, O, S) and fourth (L, P, T) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs" figureDoi="http://doi.org/10.5281/zenodo.12518542" httpUri="https://zenodo.org/record/12518542/files/figure.png" pageId="5" pageNumber="6">Fig. 3C, c</figureCitation>
) had a symmetric pattern as the result of the first cell division. Eggs containing multi-cell stages (
<figureCitation id="13558EE7FF8ED433FD7221E28AF0FA47" box="[649,767,1451,1475]" captionStart="Fig" captionStartId="5.[167,198,1528,1548]" captionTargetBox="[230,1357,237,1690]" captionTargetId="figure-88@6.[227,1361,236,1693]" captionTargetPageId="6" captionText="Fig. 3 General morphology and surface ornamentation pattern of eggs of Huffmanela persica sp. nov. in various stages of development. A–H Photomicrographs and a–h corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). A, a Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). B, b Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. C, c Two-celled mitotic stage of early embryonic development (embryonated). D, d Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers.E, e Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. F, f Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer.G, g Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). H, h Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. I–T Photomicrographs of less developed (I–L), moderately developed (M–P) and fully developed (Q–T) eggs, where the first set of images (I, M, Q) represents overview of these variously advanced eggs, and the second (J, N, R), third (K, O, S) and fourth (L, P, T) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs" figureDoi="http://doi.org/10.5281/zenodo.12518542" httpUri="https://zenodo.org/record/12518542/files/figure.png" pageId="5" pageNumber="6">Fig. 3D, d</figureCitation>
) showed further development of embryo, where the inner space of the rigid eggshell wall was partly or completely occupied by a multicellular mass. Eggs in stage II were elliptical or oblong, light yellow to light brown, with polar plugs (apparently two-layered) slightly or markedly protruding and no distinct shell layers. Egg morphometrics (
<emphasis id="B91A4E70FF8ED433FCCE25E58B4CFE47" box="[821,835,428,451]" italics="true" pageId="5" pageNumber="6">n</emphasis>
= 40) obtained in stage II: total length (inclusive of protruding polar plugs and UL) 46.79–65.84 (58.26 ± 4.83); total length (exclusive of protruding part of polar plugs and UL) 43.24–61.85 (54.08 ± 4.85); total width (with UL) 31.51–40.80 (35.43 ± 2.23); total width (without UL) 30.35–39.52 (33.57 ± 2.35); shell thickness (with UL) 3.43–8.44 (5.20 ± 1.11); shell thickness (without UL) 2.70–7.57 (4.28 ± 1.14); polar plug width 7.52–15.76 (9.78 ± 1.65).
</paragraph>
<paragraph id="8BD19262FF8ED433FCD626A48BBCFC80" blockId="5.[813,1437,749,1443]" box="[813,947,749,772]" pageId="5" pageNumber="6">
<heading id="D099250EFF8ED433FCD626A48BBCFC80" bold="true" box="[813,947,749,772]" fontSize="9" level="2" pageId="5" pageNumber="6" reason="7">
<emphasis id="B91A4E70FF8ED433FCD626A48BBCFC80" bold="true" box="[813,947,749,772]" italics="true" pageId="5" pageNumber="6">Stage III eggs</emphasis>
</heading>
</paragraph>
<paragraph id="8BD19262FF8ED433FCD627458D70FA27" blockId="5.[813,1437,749,1443]" pageId="5" pageNumber="6">
Eggs in stage III (
<figureCitation id="13558EE7FF8ED433FC0C27428C98FCA7" box="[1015,1175,779,803]" captionStart="Fig" captionStartId="5.[167,198,1528,1548]" captionTargetBox="[230,1357,237,1690]" captionTargetId="figure-88@6.[227,1361,236,1693]" captionTargetPageId="6" captionText="Fig. 3 General morphology and surface ornamentation pattern of eggs of Huffmanela persica sp. nov. in various stages of development. A–H Photomicrographs and a–h corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). A, a Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). B, b Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. C, c Two-celled mitotic stage of early embryonic development (embryonated). D, d Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers.E, e Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. F, f Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer.G, g Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). H, h Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. I–T Photomicrographs of less developed (I–L), moderately developed (M–P) and fully developed (Q–T) eggs, where the first set of images (I, M, Q) represents overview of these variously advanced eggs, and the second (J, N, R), third (K, O, S) and fourth (L, P, T) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs" figureDoi="http://doi.org/10.5281/zenodo.12518542" httpUri="https://zenodo.org/record/12518542/files/figure.png" pageId="5" pageNumber="6">Fig. 3E, e; F, f</figureCitation>
) demonstrating higher development of embryo were characterized by possessing spatially differentiated forms (bean-like or tadpole-like embryos; 
<figureCitation id="13558EE7FF8ED433FC3027228C6AFC07" box="[971,1125,875,899]" captionStart="Fig" captionStartId="5.[167,198,1528,1548]" captionTargetBox="[230,1357,237,1690]" captionTargetId="figure-88@6.[227,1361,236,1693]" captionTargetPageId="6" captionText="Fig. 3 General morphology and surface ornamentation pattern of eggs of Huffmanela persica sp. nov. in various stages of development. A–H Photomicrographs and a–h corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). A, a Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). B, b Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. C, c Two-celled mitotic stage of early embryonic development (embryonated). D, d Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers.E, e Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. F, f Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer.G, g Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). H, h Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. I–T Photomicrographs of less developed (I–L), moderately developed (M–P) and fully developed (Q–T) eggs, where the first set of images (I, M, Q) represents overview of these variously advanced eggs, and the second (J, N, R), third (K, O, S) and fourth (L, P, T) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs" figureDoi="http://doi.org/10.5281/zenodo.12518542" httpUri="https://zenodo.org/record/12518542/files/figure.png" pageId="5" pageNumber="6">Fig. 3E, e; F, f</figureCitation>
), two easily distinguishable apparent shell layers and mild to moderate degrees of embryonic bending within the eggshell. Eggs in stage III elliptical or oblong in shape, brown in color, containing two protruding polar plugs, with bilayer shell inclusive of a thin and light (hyaline) inner layer alongside with a thick and dark outer layer (this layering is an optical illusion under light microscopy caused by the birefringent properties of chitin; see Bond and Huffman, 2023) [
<bibRefCitation id="EFFFEF93FF8ED433FA8F20228D81FB07" author="Bond AT &amp; Huffman DG" box="[1396,1422,1131,1155]" pageId="5" pageNumber="6" refId="ref12658" refString="25. Bond AT, Huffman DG. Nematode eggshells: a new anatomical and terminological framework, with a critical review of relevant literature and suggested guidelines for the interpretation and reporting of eggshell imagery. Parasite. 2023; 30: 6." type="journal volume" year="2023">25</bibRefCitation>
]. Morphometric measurements of eggs (
<emphasis id="B91A4E70FF8ED433FB2C20C58CEAFB27" box="[1239,1253,1164,1187]" italics="true" pageId="5" pageNumber="6">n</emphasis>
= 27) in stage III are summarized as follows: total length (with protruding polar plugs and UL) 59.39–70.98 (62.44 ± 2.60); total length (without protruding part of polar plugs and UL) 54.75–61.67 (57.39 ± 1.94); total width (with UL) 32.46– 44.75 (34.95 ± 3.03); total width (without UL) 30.62– 43.30 (32.88 ± 3.05); shell thickness (with UL) 3.81–5.73 (4.90 ± 0.52); shell thickness (without UL) 2.92–5.02 (3.77 ± 0.46); polar plug width 8.18–11.09 (9.39 ± 0.68).
</paragraph>
<footnote id="E8758E6CFF8ED433FF5C2194898EFA75" box="[167,385,1501,1521]" pageId="5" pageNumber="6">
<paragraph id="8BD19262FF8ED433FF5C2194898EFA75" blockId="5.[167,385,1501,1521]" box="[167,385,1501,1521]" pageId="5" pageNumber="6">(See figure on next page.)</paragraph>
</footnote>
<caption id="DF11C2EAFF8ED433FF5C21B18A50F805" ID-DOI="http://doi.org/10.5281/zenodo.12518542" ID-Zenodo-Dep="12518542" httpUri="https://zenodo.org/record/12518542/files/figure.png" pageId="5" pageNumber="6" startId="5.[167,198,1528,1548]" targetBox="[230,1357,237,1690]" targetPageId="6">
<paragraph id="8BD19262FF8ED433FF5C21B18A50F805" blockId="5.[167,1413,1528,1921]" pageId="5" pageNumber="6">
<figureCitation id="13558EE7FF8ED433FF5C21B188D5F988" box="[167,218,1528,1548]" captionStart="Fig" captionStartId="6.[167,198,1705,1725]" captionTargetBox="[230,1357,237,1690]" captionTargetId="figure-88@6.[227,1361,236,1693]" captionTargetPageId="6" captionText="Fig. 3 (See legend on previous page.)" figureDoi="http://doi.org/10.5281/zenodo.11552138" httpUri="https://zenodo.org/record/11552138/files/figure.png" pageId="5" pageNumber="6">
<emphasis id="B91A4E70FF8ED433FF5C21B188D5F988" bold="true" box="[167,218,1528,1548]" pageId="5" pageNumber="6">Fig. 3</emphasis>
</figureCitation>
General morphology and surface ornamentation pattern of eggs of 
<taxonomicName id="4C6EE9E1FF8ED433FCDF21B18BCDF988" authority="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh, 2023" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[804,962,1528,1548]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="5" pageNumber="6" phylum="Nematoda" rank="species" species="persica" status="sp. nov.">
<emphasis id="B91A4E70FF8ED433FCDF21B18BCDF988" box="[804,962,1528,1548]" italics="true" pageId="5" pageNumber="6">Huffmanela persica</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A229F30BFF8ED433FC3C21B18C08F988" box="[967,1031,1528,1548]" pageId="5" pageNumber="6" rank="species">sp. nov.</taxonomicNameLabel>
in various stages of development. 
<emphasis id="B91A4E70FF8ED433FAD621B18D5CF988" bold="true" box="[1325,1363,1528,1548]" pageId="5" pageNumber="6">A–H</emphasis>
Photomicrographs and 
<emphasis id="B91A4E70FF8ED433FE8B225B899EF9A2" bold="true" box="[368,401,1554,1574]" pageId="5" pageNumber="6">a–h</emphasis>
corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). 
<emphasis id="B91A4E70FF8ED433FB02225B8D08F9A2" bold="true" box="[1273,1287,1554,1574]" pageId="5" pageNumber="6">A</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FAF4225B8D15F9A2" bold="true" box="[1295,1306,1554,1574]" pageId="5" pageNumber="6">a</emphasis>
Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). 
<emphasis id="B91A4E70FF8ED433FC8322018B8BF9D8" bold="true" box="[888,900,1608,1628]" pageId="5" pageNumber="6">B</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FC7722018B97F9D8" bold="true" box="[908,920,1608,1628]" pageId="5" pageNumber="6">b</emphasis>
Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. 
<emphasis id="B91A4E70FF8ED433FD90222B8A77F9F2" bold="true" box="[619,632,1634,1654]" pageId="5" pageNumber="6">C</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FD7B222B8A85F9F2" bold="true" box="[640,650,1634,1654]" pageId="5" pageNumber="6">c</emphasis>
Two-celled mitotic stage of early embryonic development (embryonated). 
<emphasis id="B91A4E70FF8ED433FAF3222B8D18F9F2" bold="true" box="[1288,1303,1634,1654]" pageId="5" pageNumber="6">D</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FAE4222B8D24F9F2" bold="true" box="[1311,1323,1634,1654]" pageId="5" pageNumber="6">d</emphasis>
Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers. 
<emphasis id="B91A4E70FF8ED433FF2422D188E5F928" bold="true" box="[223,234,1688,1708]" pageId="5" pageNumber="6">E</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FF0822D188F1F928" bold="true" box="[243,254,1688,1708]" pageId="5" pageNumber="6">e</emphasis>
Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. 
<emphasis id="B91A4E70FF8ED433FF2C22FB88EDF942" bold="true" box="[215,226,1714,1734]" pageId="5" pageNumber="6">F</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FF1122FB88FEF942" bold="true" box="[234,241,1714,1734]" pageId="5" pageNumber="6">f</emphasis>
Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer. 
<emphasis id="B91A4E70FF8ED433FBE322FB8C29F942" bold="true" box="[1048,1062,1714,1734]" pageId="5" pageNumber="6">G</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FBD422FB8C34F942" bold="true" box="[1071,1083,1714,1734]" pageId="5" pageNumber="6">g</emphasis>
Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). 
<emphasis id="B91A4E70FF8ED433FCA322848B69F965" bold="true" box="[856,870,1741,1761]" pageId="5" pageNumber="6">H</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FC9522848B75F965" bold="true" box="[878,890,1741,1761]" pageId="5" pageNumber="6">h</emphasis>
Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. 
<emphasis id="B91A4E70FF8ED433FDB122A18A6AF978" bold="true" box="[586,613,1768,1788]" pageId="5" pageNumber="6">I–T</emphasis>
Photomicrographs of less developed (
<emphasis id="B91A4E70FF8ED433FC5722A18BC8F978" bold="true" box="[940,967,1768,1788]" pageId="5" pageNumber="6">I–L</emphasis>
), moderately developed (
<emphasis id="B91A4E70FF8ED433FB5A22A18CC6F978" bold="true" box="[1185,1225,1768,1788]" pageId="5" pageNumber="6">M–P</emphasis>
) and fully developed (
<emphasis id="B91A4E70FF8ED433FF56234B88DDF892" bold="true" box="[173,210,1794,1814]" pageId="5" pageNumber="6">Q–T</emphasis>
) eggs, where the first set of images (
<emphasis id="B91A4E70FF8ED433FDF3234B8A01F892" bold="true" box="[520,526,1794,1814]" pageId="5" pageNumber="6">I</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FDED234B8A27F892" bold="true" box="[534,552,1794,1814]" pageId="5" pageNumber="6">M</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FDCB234B8A30F892" bold="true" box="[560,575,1794,1814]" pageId="5" pageNumber="6">Q</emphasis>
) represents overview of these variously advanced eggs, and the second (
<emphasis id="B91A4E70FF8ED433FB50234B8CBCF892" bold="true" box="[1195,1203,1794,1814]" pageId="5" pageNumber="6">J</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FB40234B8CC6F892" bold="true" box="[1211,1225,1794,1814]" pageId="5" pageNumber="6">N</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FB29234B8CD1F892" bold="true" box="[1234,1246,1794,1814]" pageId="5" pageNumber="6">R</emphasis>
), third (
<emphasis id="B91A4E70FF8ED433FAE4234B8D24F892" bold="true" box="[1311,1323,1794,1814]" pageId="5" pageNumber="6">K</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FACF234B8D4CF892" bold="true" box="[1332,1347,1794,1814]" pageId="5" pageNumber="6">O</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FAB0234B8D59F892" bold="true" box="[1355,1366,1794,1814]" pageId="5" pageNumber="6">S</emphasis>
) and fourth (
<emphasis id="B91A4E70FF8ED433FF1D235488FEF8B5" bold="true" box="[230,241,1821,1841]" pageId="5" pageNumber="6">L</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FF022354890AF8B5" bold="true" box="[249,261,1821,1841]" pageId="5" pageNumber="6">P</emphasis>
, 
<emphasis id="B91A4E70FF8ED433FEF523548916F8B5" bold="true" box="[270,281,1821,1841]" pageId="5" pageNumber="6">T</emphasis>
) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs
</paragraph>
</caption>
<caption id="DF11C2EAFF8DD430FF5C22E089E3F939" ID-DOI="http://doi.org/10.5281/zenodo.11552138" ID-Zenodo-Dep="11552138" box="[167,492,1705,1725]" httpUri="https://zenodo.org/record/11552138/files/figure.png" pageId="6" pageNumber="7" startId="6.[167,198,1705,1725]" targetBox="[230,1357,237,1690]" targetPageId="6" targetType="figure">
<paragraph id="8BD19262FF8DD430FF5C22E089E3F939" blockId="6.[167,492,1705,1725]" box="[167,492,1705,1725]" pageId="6" pageNumber="7">
<emphasis id="B91A4E70FF8DD430FF5C22E088D5F939" bold="true" box="[167,218,1705,1725]" pageId="6" pageNumber="7">Fig. 3</emphasis>
(See legend on previous page.)
</paragraph>
</caption>
<paragraph id="8BD19262FF8DD430FF6C23428910F8A6" blockId="6.[151,775,1803,1921]" box="[151,287,1803,1826]" pageId="6" pageNumber="7">
<heading id="D099250EFF8DD430FF6C23428910F8A6" bold="true" box="[151,287,1803,1826]" fontSize="9" level="2" pageId="6" pageNumber="7" reason="7">
<emphasis id="B91A4E70FF8DD430FF6C23428910F8A6" bold="true" box="[151,287,1803,1826]" italics="true" pageId="6" pageNumber="7">Stage IV eggs</emphasis>
</heading>
</paragraph>
<paragraph id="8BD19262FF8DD431FF6C23608A95F977" blockId="6.[151,775,1803,1921]" lastBlockId="7.[151,775,1435,1780]" lastPageId="7" lastPageNumber="8" pageId="6" pageNumber="7">
Stage IV eggs (
<figureCitation id="13558EE7FF8DD430FEBB23608A09F8C5" box="[320,518,1833,1858]" captionStart="Fig" captionStartId="5.[167,198,1528,1548]" captionTargetBox="[230,1357,237,1690]" captionTargetId="figure-88@6.[227,1361,236,1693]" captionTargetPageId="6" captionText="Fig. 3 General morphology and surface ornamentation pattern of eggs of Huffmanela persica sp. nov. in various stages of development. A–H Photomicrographs and a–h corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). A, a Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). B, b Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. C, c Two-celled mitotic stage of early embryonic development (embryonated). D, d Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers.E, e Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. F, f Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer.G, g Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). H, h Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. I–T Photomicrographs of less developed (I–L), moderately developed (M–P) and fully developed (Q–T) eggs, where the first set of images (I, M, Q) represents overview of these variously advanced eggs, and the second (J, N, R), third (K, O, S) and fourth (L, P, T) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs" figureDoi="http://doi.org/10.5281/zenodo.12518542" httpUri="https://zenodo.org/record/12518542/files/figure.png" pageId="6" pageNumber="7">Figs. 3G, g; H, h</figureCitation>
; 
<figureCitation id="13558EE7FF8DD430FDEF23608A45F8C5" box="[532,586,1833,1857]" captionStart="Fig" captionStartId="7.[167,198,1166,1186]" captionTargetBox="[169,1420,239,1148]" captionTargetId="figure-286@7.[166,1421,236,1153]" captionTargetPageId="7" captionText="Fig. 4 Scanning electron micrographs of poorly developed (A–D) and fully developed (E–I) eggs of Huffmanela persica sp. nov. (separated from infected ovary).Less developed eggs spherical shaped (white arrow shows a shrunken and wrinkled egg) and with no evidently developed polar plugs. Fully developed eggs oblong and containing two plugs at poles. Eggs completely surrounded by a UL bearing uniformly based mammiform mounds adorned with tendril-like vermiform appendage emerging from the apex,occasionally adjoined to that of a neighboring mound (green arrowheads). Note the illusory appearance of serrated-like ridges (occasionally in the form of illusory interconnecting ridges,black arrows) in side views which is caused by overlapping of the mound bases (well observed in less developed eggs, red arrowheads). Outer surface of eggshell with irregular protuberances (yellow arrowheads)" figureDoi="http://doi.org/10.5281/zenodo.11552140" httpUri="https://zenodo.org/record/11552140/files/figure.png" pageId="6" pageNumber="7">4E–I</figureCitation>
; 
<figureCitation id="13558EE7FF8DD430FDA323608A9EF8C5" box="[600,657,1833,1857]" captionStart="Fig" captionStartId="8.[167,198,1521,1541]" captionTargetBox="[290,1304,240,1508]" captionTargetId="figure-87@8.[284,1304,236,1508]" captionTargetPageId="8" captionText="Fig. 5 Photomicrographs from histological sections of infected tissues of a daggertooth pike conger eel. A, B Histological sections of the stomach infected by eggs of Huffmanela persica sp. nov. at various stages of development as well as degenerated encysted metazoans (⁎). C, D Sections of the tunica serosa of the stomach parasitized with completely developed eggs.E Sections of the ovarian lamellae infected by both clusters of immature (➔) and developing (➤) eggs, representing immature and previtellogenic oocytes embedded within a loose fibro-granulomatous infiltration containing histiocytes and eosinophilic granular leukocytes (⁎). F Magnified view of histological section of infected ovary showing a fibro-granulomatous infiltrate surrounding clusters of eggs at different stages of development (mainly highly developed eggs, ➤). G High magnification (100×) view of a fully developed egg of H. persica (cross-sectional view) showing larva in-folded within the eggshell and a protruding polar plug at either end. H Less developed eggs with a nearly central nucleus (⁎) and thin eggshell layer and I fully developed eggs containing twisted larvae of H. persica cut on varying planes of section" figureDoi="http://doi.org/10.5281/zenodo.11552142" httpUri="https://zenodo.org/record/11552142/files/figure.png" pageId="6" pageNumber="7">5G, I</figureCitation>
) were differentiated by having brown to dark-brown color, oblong or elliptical shape, varying degrees of larval formation within egg from threefold stage (pretzel, 
<figureCitation id="13558EE7FF8DD430FAF923438D7CF8A6" box="[1282,1395,1802,1826]" captionStart="Fig" captionStartId="5.[167,198,1528,1548]" captionTargetBox="[230,1357,237,1690]" captionTargetId="figure-88@6.[227,1361,236,1693]" captionTargetPageId="6" captionText="Fig. 3 General morphology and surface ornamentation pattern of eggs of Huffmanela persica sp. nov. in various stages of development. A–H Photomicrographs and a–h corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). A, a Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). B, b Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. C, c Two-celled mitotic stage of early embryonic development (embryonated). D, d Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers.E, e Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. F, f Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer.G, g Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). H, h Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. I–T Photomicrographs of less developed (I–L), moderately developed (M–P) and fully developed (Q–T) eggs, where the first set of images (I, M, Q) represents overview of these variously advanced eggs, and the second (J, N, R), third (K, O, S) and fourth (L, P, T) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs" figureDoi="http://doi.org/10.5281/zenodo.12518542" httpUri="https://zenodo.org/record/12518542/files/figure.png" pageId="6" pageNumber="7">Fig. 3G, g</figureCitation>
) to five–sixfold stage (fully developed larva, 
<figureCitation id="13558EE7FF8DD430FB1023638D58F8C6" box="[1259,1367,1834,1858]" captionStart="Fig" captionStartId="5.[167,198,1528,1548]" captionTargetBox="[230,1357,237,1690]" captionTargetId="figure-88@6.[227,1361,236,1693]" captionTargetPageId="6" captionText="Fig. 3 General morphology and surface ornamentation pattern of eggs of Huffmanela persica sp. nov. in various stages of development. A–H Photomicrographs and a–h corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). A, a Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). B, b Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. C, c Two-celled mitotic stage of early embryonic development (embryonated). D, d Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers.E, e Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. F, f Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer.G, g Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). H, h Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. I–T Photomicrographs of less developed (I–L), moderately developed (M–P) and fully developed (Q–T) eggs, where the first set of images (I, M, Q) represents overview of these variously advanced eggs, and the second (J, N, R), third (K, O, S) and fourth (L, P, T) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs" figureDoi="http://doi.org/10.5281/zenodo.12518542" httpUri="https://zenodo.org/record/12518542/files/figure.png" pageId="6" pageNumber="7">Fig. 3H, h</figureCitation>
), dark outer layer much thicker in width and two distinctly protruding polar plugs. Morphometric data obtained from stage IV eggs (
<emphasis id="B91A4E70FF8CD431FE8021D58986FA37" box="[379,393,1436,1459]" italics="true" pageId="7" pageNumber="8">n</emphasis>
= 71) were as follows: total length (with protruding polar plugs and UL) 60.37–75.13 (66.62 ± 3.04); total length (without protruding part of polar plugs and UL) 54.37–67.86 (62.03 ± 2.94); total width (with UL) 32.54–44.63 (36.46 ± 2.65); total width (without UL) 30.96–43.39 (34.41 ± 2.78); shell thickness (with UL) 4.48–7.17 (5.63± 0.53); shell thickness (without UL) 3.51–6.11 (4.63 ± 0.55); polar plug width 8.43–12.00 (10.29 ± 0.79); larval width in fully developed eggs 6.09–9.16 (7.91 ± 0.61); larval length (
<emphasis id="B91A4E70FF8CD431FD9222F58A78F957" box="[617,631,1724,1747]" italics="true" pageId="7" pageNumber="8">n</emphasis>
= 17) in fully developed eggs 196.27–231.49 (209.51 ± 10.64).
</paragraph>
<caption id="DF11C2EAFF8CD431FF5C20C78A11FAC6" ID-DOI="http://doi.org/10.5281/zenodo.11552140" ID-Zenodo-Dep="11552140" httpUri="https://zenodo.org/record/11552140/files/figure.png" pageId="7" pageNumber="8" startId="7.[167,198,1166,1186]" targetBox="[169,1420,239,1148]" targetPageId="7" targetType="figure">
<paragraph id="8BD19262FF8CD431FF5C20C78A11FAC6" blockId="7.[167,1412,1166,1346]" pageId="7" pageNumber="8">
<emphasis id="B91A4E70FF8CD431FF5C20C788D5FB26" bold="true" box="[167,218,1166,1186]" pageId="7" pageNumber="8">Fig. 4</emphasis>
Scanning electron micrographs of poorly developed (
<emphasis id="B91A4E70FF8CD431FD5020C78ADDFB26" bold="true" box="[683,722,1166,1186]" pageId="7" pageNumber="8">A–D</emphasis>
) and fully developed (
<emphasis id="B91A4E70FF8CD431FC7420C78BA4FB26" bold="true" box="[911,939,1166,1186]" pageId="7" pageNumber="8">E–I</emphasis>
) eggs of 
<taxonomicName id="4C6EE9E1FF8CD431FC0320C78C98FB26" authority="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh, 2023" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[1016,1175,1166,1186]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="7" pageNumber="8" phylum="Nematoda" rank="species" species="persica" status="sp. nov.">
<emphasis id="B91A4E70FF8CD431FC0320C78C98FB26" box="[1016,1175,1166,1186]" italics="true" pageId="7" pageNumber="8">Huffmanela persica</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A229F30BFF8CD431FB6020C78CD3FB26" box="[1179,1244,1166,1186]" pageId="7" pageNumber="8" rank="species">sp. nov.</taxonomicNameLabel>
(separated from infected ovary).Less developed eggs spherical shaped (white arrow shows a shrunken and wrinkled egg) and with no evidently developed polar plugs. Fully developed eggs oblong and containing two plugs at poles. Eggs completely surrounded by a UL bearing uniformly based mammiform mounds adorned with tendril-like vermiform appendage emerging from the apex,occasionally adjoined to that of a neighboring mound (green arrowheads). Note the illusory appearance of serrated-like ridges (occasionally in the form of illusory interconnecting ridges,black arrows) in side views which is caused by overlapping of the mound bases (well observed in less developed eggs, red arrowheads). Outer surface of eggshell with irregular protuberances (yellow arrowheads)
</paragraph>
</caption>
<paragraph id="8BD19262FF8CD431FF6C235489CEF8B0" blockId="7.[151,775,1821,1908]" box="[151,449,1821,1844]" pageId="7" pageNumber="8">
<heading id="D099250EFF8CD431FF6C235489CEF8B0" bold="true" box="[151,449,1821,1844]" fontSize="9" level="2" pageId="7" pageNumber="8" reason="4">
<emphasis id="B91A4E70FF8CD431FF6C235489CEF8B0" bold="true" box="[151,449,1821,1844]" pageId="7" pageNumber="8">Egg surface ornamentations</emphasis>
</heading>
</paragraph>
<paragraph id="8BD19262FF8CD431FF6C23758D60F8F7" blockId="7.[151,775,1821,1908]" lastBlockId="7.[813,1437,1435,1907]" pageId="7" pageNumber="8">
Te rigid eggshell wall (or its integral outermost layer, 
<taxonomicName id="4C6EE9E1FF8CD431FF6C2312893EF8F7" authority="Ovi" authorityName="Ovi" box="[151,305,1883,1907]" class="Gastropoda" family="Amphibulimidae" genus="Pellicula" kingdom="Animalia" order="Stylommatophora" pageId="7" pageNumber="8" phylum="Mollusca" rank="genus">
<emphasis id="B91A4E70FF8CD431FF6C2312893EF8F7" box="[151,305,1883,1907]" italics="true" pageId="7" pageNumber="8">Pellicula Ovi</emphasis>
</taxonomicName>
) at all stages adorned with irregular protuberances on the surface (
<figureCitation id="13558EE7FF8CD431FB6F21D28D40FA37" box="[1172,1359,1435,1459]" captionStart="Fig" captionStartId="7.[167,198,1166,1186]" captionTargetBox="[169,1420,239,1148]" captionTargetId="figure-286@7.[166,1421,236,1153]" captionTargetPageId="7" captionText="Fig. 4 Scanning electron micrographs of poorly developed (A–D) and fully developed (E–I) eggs of Huffmanela persica sp. nov. (separated from infected ovary).Less developed eggs spherical shaped (white arrow shows a shrunken and wrinkled egg) and with no evidently developed polar plugs. Fully developed eggs oblong and containing two plugs at poles. Eggs completely surrounded by a UL bearing uniformly based mammiform mounds adorned with tendril-like vermiform appendage emerging from the apex,occasionally adjoined to that of a neighboring mound (green arrowheads). Note the illusory appearance of serrated-like ridges (occasionally in the form of illusory interconnecting ridges,black arrows) in side views which is caused by overlapping of the mound bases (well observed in less developed eggs, red arrowheads). Outer surface of eggshell with irregular protuberances (yellow arrowheads)" figureDoi="http://doi.org/10.5281/zenodo.11552140" httpUri="https://zenodo.org/record/11552140/files/figure.png" pageId="7" pageNumber="8">Fig. 4B–D, H, I</figureCitation>
). Eggs thoroughly surrounded by a thin transparent UL decorated with closely and regularly spaced mammiform mounds (superficial projections, 
<figureCitation id="13558EE7FF8CD431FB6321B28D01F997" box="[1176,1294,1531,1555]" captionStart="Fig" captionStartId="5.[167,198,1528,1548]" captionTargetBox="[230,1357,237,1690]" captionTargetId="figure-88@6.[227,1361,236,1693]" captionTargetPageId="6" captionText="Fig. 3 General morphology and surface ornamentation pattern of eggs of Huffmanela persica sp. nov. in various stages of development. A–H Photomicrographs and a–h corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). A, a Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). B, b Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. C, c Two-celled mitotic stage of early embryonic development (embryonated). D, d Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers.E, e Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. F, f Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer.G, g Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). H, h Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. I–T Photomicrographs of less developed (I–L), moderately developed (M–P) and fully developed (Q–T) eggs, where the first set of images (I, M, Q) represents overview of these variously advanced eggs, and the second (J, N, R), third (K, O, S) and fourth (L, P, T) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs" figureDoi="http://doi.org/10.5281/zenodo.12518542" httpUri="https://zenodo.org/record/12518542/files/figure.png" pageId="7" pageNumber="8">Figs. 3 I–T</figureCitation>
, 
<figureCitation id="13558EE7FF8CD431FAE121B28D9BF997" box="[1306,1428,1531,1555]" captionStart="Fig" captionStartId="7.[167,198,1166,1186]" captionTargetBox="[169,1420,239,1148]" captionTargetId="figure-286@7.[166,1421,236,1153]" captionTargetPageId="7" captionText="Fig. 4 Scanning electron micrographs of poorly developed (A–D) and fully developed (E–I) eggs of Huffmanela persica sp. nov. (separated from infected ovary).Less developed eggs spherical shaped (white arrow shows a shrunken and wrinkled egg) and with no evidently developed polar plugs. Fully developed eggs oblong and containing two plugs at poles. Eggs completely surrounded by a UL bearing uniformly based mammiform mounds adorned with tendril-like vermiform appendage emerging from the apex,occasionally adjoined to that of a neighboring mound (green arrowheads). Note the illusory appearance of serrated-like ridges (occasionally in the form of illusory interconnecting ridges,black arrows) in side views which is caused by overlapping of the mound bases (well observed in less developed eggs, red arrowheads). Outer surface of eggshell with irregular protuberances (yellow arrowheads)" figureDoi="http://doi.org/10.5281/zenodo.11552140" httpUri="https://zenodo.org/record/11552140/files/figure.png" pageId="7" pageNumber="8">4B–D, F, G</figureCitation>
) appearing to form pointed serrated-like ridges (
<figureCitation id="13558EE7FF8CD431FAB622528B6DF9D7" captionStart="Fig" captionStartId="5.[167,198,1528,1548]" captionTargetBox="[230,1357,237,1690]" captionTargetId="figure-88@6.[227,1361,236,1693]" captionTargetPageId="6" captionText="Fig. 3 General morphology and surface ornamentation pattern of eggs of Huffmanela persica sp. nov. in various stages of development. A–H Photomicrographs and a–h corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). A, a Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). B, b Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. C, c Two-celled mitotic stage of early embryonic development (embryonated). D, d Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers.E, e Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. F, f Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer.G, g Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). H, h Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. I–T Photomicrographs of less developed (I–L), moderately developed (M–P) and fully developed (Q–T) eggs, where the first set of images (I, M, Q) represents overview of these variously advanced eggs, and the second (J, N, R), third (K, O, S) and fourth (L, P, T) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs" figureDoi="http://doi.org/10.5281/zenodo.12518542" httpUri="https://zenodo.org/record/12518542/files/figure.png" pageId="7" pageNumber="8">Fig. 3J, N, R</figureCitation>
) (occasionally appearing as interconnecting ridges; 
<figureCitation id="13558EE7FF8CD431FCD622128B72F9F6" box="[813,893,1627,1651]" captionStart="Fig" captionStartId="5.[167,198,1528,1548]" captionTargetBox="[230,1357,237,1690]" captionTargetId="figure-88@6.[227,1361,236,1693]" captionTargetPageId="6" captionText="Fig. 3 General morphology and surface ornamentation pattern of eggs of Huffmanela persica sp. nov. in various stages of development. A–H Photomicrographs and a–h corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). A, a Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). B, b Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. C, c Two-celled mitotic stage of early embryonic development (embryonated). D, d Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers.E, e Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. F, f Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer.G, g Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). H, h Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. I–T Photomicrographs of less developed (I–L), moderately developed (M–P) and fully developed (Q–T) eggs, where the first set of images (I, M, Q) represents overview of these variously advanced eggs, and the second (J, N, R), third (K, O, S) and fourth (L, P, T) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs" figureDoi="http://doi.org/10.5281/zenodo.12518542" httpUri="https://zenodo.org/record/12518542/files/figure.png" pageId="7" pageNumber="8">Figs. 3J</figureCitation>
, 
<figureCitation id="13558EE7FF8CD431FC7322128BA6F9F7" box="[904,937,1627,1651]" captionStart="Fig" captionStartId="7.[167,198,1166,1186]" captionTargetBox="[169,1420,239,1148]" captionTargetId="figure-286@7.[166,1421,236,1153]" captionTargetPageId="7" captionText="Fig. 4 Scanning electron micrographs of poorly developed (A–D) and fully developed (E–I) eggs of Huffmanela persica sp. nov. (separated from infected ovary).Less developed eggs spherical shaped (white arrow shows a shrunken and wrinkled egg) and with no evidently developed polar plugs. Fully developed eggs oblong and containing two plugs at poles. Eggs completely surrounded by a UL bearing uniformly based mammiform mounds adorned with tendril-like vermiform appendage emerging from the apex,occasionally adjoined to that of a neighboring mound (green arrowheads). Note the illusory appearance of serrated-like ridges (occasionally in the form of illusory interconnecting ridges,black arrows) in side views which is caused by overlapping of the mound bases (well observed in less developed eggs, red arrowheads). Outer surface of eggshell with irregular protuberances (yellow arrowheads)" figureDoi="http://doi.org/10.5281/zenodo.11552140" httpUri="https://zenodo.org/record/11552140/files/figure.png" pageId="7" pageNumber="8">4D</figureCitation>
) on the egg surface (the appearance of ridges is most likely a geometric illusion; see 
<figureCitation id="13558EE7FF8CD431FB1B22328D49F917" box="[1248,1350,1659,1683]" captionStart-0="Fig" captionStart-1="Fig" captionStart-2="Fig" captionStartId-0="2.[167,198,1741,1761]" captionStartId-1="4.[829,860,1097,1117]" captionStartId-2="5.[167,198,1528,1548]" captionTargetBox-0="[178,750,1297,1726]" captionTargetBox-1="[840,1411,238,1082]" captionTargetBox-2="[230,1357,237,1690]" captionTargetId-0="figure-727@2.[176,751,1296,1729]" captionTargetId-1="figure-450@4.[837,1412,236,1084]" captionTargetId-2="figure-88@6.[227,1361,236,1693]" captionTargetPageId-0="2" captionTargetPageId-1="4" captionTargetPageId-2="6" captionText-0="Fig. 1 Representation of an advanced egg of Huffmanela persica sp. nov. with measurements considered in this study.Total length with protruding polar plug and UL (black line); total length without protruding polar plug and UL (red line); total width with UL (yellow line); total width without UL (light blue line); shell thickness with UL (green line); shell thickness without UL (white line); polar plug width (dark blue line)" captionText-1="Fig. 2 Macroscopic and microscopic appearance of fully developed eggs of Huffmanela persica sp. nov. A Grossly visible lesions of eggs previously deposited by adult forms of H.persica sp. nov. in the form of dark spots of various size within the infected tissues (ovary and serosa of stomach) of Muraenesox cinereus. B Wet mount prepared from infected ovary illustrating variously oriented advanced eggs of H.persica sp.nov.within the egg clusters" captionText-2="Fig. 3 General morphology and surface ornamentation pattern of eggs of Huffmanela persica sp. nov. in various stages of development. A–H Photomicrographs and a–h corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). A, a Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). B, b Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. C, c Two-celled mitotic stage of early embryonic development (embryonated). D, d Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers.E, e Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. F, f Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer.G, g Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). H, h Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. I–T Photomicrographs of less developed (I–L), moderately developed (M–P) and fully developed (Q–T) eggs, where the first set of images (I, M, Q) represents overview of these variously advanced eggs, and the second (J, N, R), third (K, O, S) and fourth (L, P, T) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs" figureDoi-0="http://doi.org/10.5281/zenodo.11552132" figureDoi-1="http://doi.org/10.5281/zenodo.11552134" figureDoi-2="http://doi.org/10.5281/zenodo.12518542" httpUri-0="https://zenodo.org/record/11552132/files/figure.png" httpUri-1="https://zenodo.org/record/11552134/files/figure.png" httpUri-2="https://zenodo.org/record/12518542/files/figure.png" pageId="7" pageNumber="8">Figs. 1–3</figureCitation>
, 7, 8 of Žďárská et al. 2001, Fig. 30 of Bond, 2020 and Fig. 24 of Bond and Huffman, 2023) [
<bibRefCitation id="EFFFEF93FF8CD431FB9222F28C8CF957" author="Bond AT &amp; Huffman DG" box="[1129,1155,1723,1747]" pageId="7" pageNumber="8" refId="ref12658" refString="25. Bond AT, Huffman DG. Nematode eggshells: a new anatomical and terminological framework, with a critical review of relevant literature and suggested guidelines for the interpretation and reporting of eggshell imagery. Parasite. 2023; 30: 6." type="journal volume" year="2023">25</bibRefCitation>
], resulting in manifestation of a fine superficial sculpture on the surface of eggs examined with light microscope (
<figureCitation id="13558EE7FF8CD431FB6C22B28D2FF897" box="[1175,1312,1787,1811]" captionStart="Fig" captionStartId="5.[167,198,1528,1548]" captionTargetBox="[230,1357,237,1690]" captionTargetId="figure-88@6.[227,1361,236,1693]" captionTargetPageId="6" captionText="Fig. 3 General morphology and surface ornamentation pattern of eggs of Huffmanela persica sp. nov. in various stages of development. A–H Photomicrographs and a–h corresponding line drawings of individual eggs at different stages of development (scale bars: 20 µm). A, a Eggs in stage I at very early stage,probably meiosis I, with a spherical nucleus in granular cytoplasm and incompletely developed chitinous layer and polar plugs (note early appearance of superficial projections of UL already apparent). B, b Eggs in stage II at later stage of development (probably meiosis II); chitin deposition appears to be complete. C, c Two-celled mitotic stage of early embryonic development (embryonated). D, d Later multicellular stage of embryonic development; chitinous layer still uniformly translucent with no apparent division into outer and inner chitinous layers.E, e Eggs in stage III with bean-like embryo and chitinous layer appearing two-layered under bright-field (light) microscopy with darker inner layer. F, f Tadpole-like embryos with UL appearing to have been partially dislodged from chitinous layer.G, g Eggs in stage IV with darker-brown shell; embryo now vermiform (larvated) and in-folded three times (pretzel stage). H, h Later stage IV egg with chitinous layer very dark brown; larva nearing final development and folded 5–6 times. I–T Photomicrographs of less developed (I–L), moderately developed (M–P) and fully developed (Q–T) eggs, where the first set of images (I, M, Q) represents overview of these variously advanced eggs, and the second (J, N, R), third (K, O, S) and fourth (L, P, T) series of images focus on the pattern of their surface ornamentation by adjusting the focal plane. Black and yellow arrows represent illusions of superficial ridges (well demonstrated in less developed eggs; occasionally appearing as interconnecting ridges, blue arrowhead) and sculptures on the egg surface, respectively. Green arrowheads exhibit irregular protuberances on the eggshell surface.Red arrowheads indicate an illusory spinous appearance in fully developed eggs" figureDoi="http://doi.org/10.5281/zenodo.12518542" httpUri="https://zenodo.org/record/12518542/files/figure.png" pageId="7" pageNumber="8">Fig. 3K, O, S</figureCitation>
). UL mammiform mounds predominantly with a tendril-like vermiform appendage extending from the tip, sometimes adjoined to that of a neighboring mound (
<figureCitation id="13558EE7FF8CD431FB0823128D6CF8F7" box="[1267,1379,1883,1907]" captionStart="Fig" captionStartId="7.[167,198,1166,1186]" captionTargetBox="[169,1420,239,1148]" captionTargetId="figure-286@7.[166,1421,236,1153]" captionTargetPageId="7" captionText="Fig. 4 Scanning electron micrographs of poorly developed (A–D) and fully developed (E–I) eggs of Huffmanela persica sp. nov. (separated from infected ovary).Less developed eggs spherical shaped (white arrow shows a shrunken and wrinkled egg) and with no evidently developed polar plugs. Fully developed eggs oblong and containing two plugs at poles. Eggs completely surrounded by a UL bearing uniformly based mammiform mounds adorned with tendril-like vermiform appendage emerging from the apex,occasionally adjoined to that of a neighboring mound (green arrowheads). Note the illusory appearance of serrated-like ridges (occasionally in the form of illusory interconnecting ridges,black arrows) in side views which is caused by overlapping of the mound bases (well observed in less developed eggs, red arrowheads). Outer surface of eggshell with irregular protuberances (yellow arrowheads)" figureDoi="http://doi.org/10.5281/zenodo.11552140" httpUri="https://zenodo.org/record/11552140/files/figure.png" pageId="7" pageNumber="8">Fig. 4A–F</figureCitation>
).
</paragraph>
<caption id="DF11C2EAFF83D43EFF5C21B88A95F95E" ID-DOI="http://doi.org/10.5281/zenodo.11552142" ID-Zenodo-Dep="11552142" httpUri="https://zenodo.org/record/11552142/files/figure.png" pageId="8" pageNumber="9" startId="8.[167,198,1521,1541]" targetBox="[290,1304,240,1508]" targetPageId="8" targetType="figure">
<paragraph id="8BD19262FF83D43EFF5C21B88A95F95E" blockId="8.[167,1412,1521,1754]" pageId="8" pageNumber="9">
<emphasis id="B91A4E70FF83D43EFF5C21B888D5F981" bold="true" box="[167,218,1521,1541]" pageId="8" pageNumber="9">Fig. 5</emphasis>
Photomicrographs from histological sections of infected tissues of a daggertooth pike conger eel. 
<emphasis id="B91A4E70FF83D43EFBD821B88C3EF981" bold="true" box="[1059,1073,1521,1541]" pageId="8" pageNumber="9">A</emphasis>
, 
<emphasis id="B91A4E70FF83D43EFBC221B88C4AF981" bold="true" box="[1081,1093,1521,1541]" pageId="8" pageNumber="9">B</emphasis>
Histological sections of the stomach infected by eggs of 
<taxonomicName id="4C6EE9E1FF83D43EFEB4224589E2F9A4" authority="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh, 2023" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[335,493,1548,1568]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="8" pageNumber="9" phylum="Nematoda" rank="species" species="persica" status="sp. nov.">
<emphasis id="B91A4E70FF83D43EFEB4224589E2F9A4" box="[335,493,1548,1568]" italics="true" pageId="8" pageNumber="9">Huffmanela persica</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A229F30BFF83D43EFE0922458A3CF9A4" box="[498,563,1548,1568]" pageId="8" pageNumber="9" rank="species">sp. nov.</taxonomicNameLabel>
at various stages of development as well as degenerated encysted metazoans (⁎). 
<emphasis id="B91A4E70FF83D43EFB1522458CF4F9A4" bold="true" box="[1262,1275,1548,1568]" pageId="8" pageNumber="9">C</emphasis>
, 
<emphasis id="B91A4E70FF83D43EFAF822458D1DF9A4" bold="true" box="[1283,1298,1548,1568]" pageId="8" pageNumber="9">D</emphasis>
Sections of the tunica serosa of the stomach parasitized with completely developed eggs. 
<emphasis id="B91A4E70FF83D43EFCAC226F8B6DF9BE" bold="true" box="[855,866,1574,1594]" pageId="8" pageNumber="9">E</emphasis>
Sections of the ovarian lamellae infected by both clusters of immature (➔) and developing (➤) eggs, representing immature and previtellogenic oocytes embedded within a loose fibro-granulomatous infiltration containing histiocytes and eosinophilic granular leukocytes (⁎). 
<emphasis id="B91A4E70FF83D43EFCE522158B26F9F4" bold="true" box="[798,809,1628,1648]" pageId="8" pageNumber="9">F</emphasis>
Magnified view of histological section of infected ovary showing a fibro-granulomatous infiltrate surrounding clusters of eggs at different stages of development (mainly highly developed eggs, ➤). 
<emphasis id="B91A4E70FF83D43EFAFC223F8D1AF90E" bold="true" box="[1287,1301,1654,1674]" pageId="8" pageNumber="9">G</emphasis>
High magnification (100×) view of a fully developed egg of 
<taxonomicName id="4C6EE9E1FF83D43EFD8822D88ACEF921" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[627,705,1681,1701]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="8" pageNumber="9" phylum="Nematoda" rank="species" species="persica">
<emphasis id="B91A4E70FF83D43EFD8822D88ACEF921" box="[627,705,1681,1701]" italics="true" pageId="8" pageNumber="9">H. persica</emphasis>
</taxonomicName>
(cross-sectional view) showing larva in-folded within the eggshell and a protruding polar plug at either end. 
<emphasis id="B91A4E70FF83D43EFE8322E58989F944" bold="true" box="[376,390,1708,1728]" pageId="8" pageNumber="9">H</emphasis>
Less developed eggs with a nearly central nucleus (⁎) and thin eggshell layer and 
<emphasis id="B91A4E70FF83D43EFBC422E58C4AF944" bold="true" box="[1087,1093,1708,1728]" pageId="8" pageNumber="9">I</emphasis>
fully developed eggs containing twisted larvae of 
<taxonomicName id="4C6EE9E1FF83D43EFEC3228F8989F95E" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[312,390,1734,1754]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="8" pageNumber="9" phylum="Nematoda" rank="species" species="persica">
<emphasis id="B91A4E70FF83D43EFEC3228F8989F95E" box="[312,390,1734,1754]" italics="true" pageId="8" pageNumber="9">H. persica</emphasis>
</taxonomicName>
cut on varying planes of section
</paragraph>
</caption>
<paragraph id="8BD19262FF83D43EFF6C23538965F8B5" blockId="8.[151,775,1818,1937]" box="[151,362,1818,1841]" pageId="8" pageNumber="9">
<heading id="D099250EFF83D43EFF6C23538965F8B5" bold="true" box="[151,362,1818,1841]" fontSize="9" level="2" pageId="8" pageNumber="9" reason="4">
<emphasis id="B91A4E70FF83D43EFF6C23538965F8B5" bold="true" box="[151,362,1818,1841]" pageId="8" pageNumber="9">Pathological lesions</emphasis>
</heading>
</paragraph>
<paragraph id="8BD19262FF83D43FFF6C237089BFFE67" blockId="8.[151,775,1818,1937]" lastBlockId="9.[151,775,235,483]" lastPageId="9" lastPageNumber="10" pageId="8" pageNumber="9">
Te serosal surface of the stomach in infected fish contained eggs of 
<taxonomicName id="4C6EE9E1FF83D43EFEBE231389B7F8F5" authority="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh, 2023" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[325,440,1882,1905]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="8" pageNumber="9" phylum="Nematoda" rank="species" species="persica" status="sp. nov.">
<emphasis id="B91A4E70FF83D43EFEBE231389B7F8F5" box="[325,440,1882,1905]" italics="true" pageId="8" pageNumber="9">H. persica</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A229F30BFF83D43EFE3F23108A13F8F4" box="[452,540,1881,1904]" pageId="8" pageNumber="9" rank="species">sp. nov.</taxonomicNameLabel>
at various stages of development (
<figureCitation id="13558EE7FF83D43EFECE233089A5F815" box="[309,426,1913,1937]" captionStart="Fig" captionStartId="8.[167,198,1521,1541]" captionTargetBox="[290,1304,240,1508]" captionTargetId="figure-87@8.[284,1304,236,1508]" captionTargetPageId="8" captionText="Fig. 5 Photomicrographs from histological sections of infected tissues of a daggertooth pike conger eel. A, B Histological sections of the stomach infected by eggs of Huffmanela persica sp. nov. at various stages of development as well as degenerated encysted metazoans (⁎). C, D Sections of the tunica serosa of the stomach parasitized with completely developed eggs.E Sections of the ovarian lamellae infected by both clusters of immature (➔) and developing (➤) eggs, representing immature and previtellogenic oocytes embedded within a loose fibro-granulomatous infiltration containing histiocytes and eosinophilic granular leukocytes (⁎). F Magnified view of histological section of infected ovary showing a fibro-granulomatous infiltrate surrounding clusters of eggs at different stages of development (mainly highly developed eggs, ➤). G High magnification (100×) view of a fully developed egg of H. persica (cross-sectional view) showing larva in-folded within the eggshell and a protruding polar plug at either end. H Less developed eggs with a nearly central nucleus (⁎) and thin eggshell layer and I fully developed eggs containing twisted larvae of H. persica cut on varying planes of section" figureDoi="http://doi.org/10.5281/zenodo.11552142" httpUri="https://zenodo.org/record/11552142/files/figure.png" pageId="8" pageNumber="9">Fig. 5A–D</figureCitation>
) and degenerated encapsulated metazoans (
<figureCitation id="13558EE7FF83D43EFC4823508C28F8B5" box="[947,1063,1817,1842]" captionStart="Fig" captionStartId="8.[167,198,1521,1541]" captionTargetBox="[290,1304,240,1508]" captionTargetId="figure-87@8.[284,1304,236,1508]" captionTargetPageId="8" captionText="Fig. 5 Photomicrographs from histological sections of infected tissues of a daggertooth pike conger eel. A, B Histological sections of the stomach infected by eggs of Huffmanela persica sp. nov. at various stages of development as well as degenerated encysted metazoans (⁎). C, D Sections of the tunica serosa of the stomach parasitized with completely developed eggs.E Sections of the ovarian lamellae infected by both clusters of immature (➔) and developing (➤) eggs, representing immature and previtellogenic oocytes embedded within a loose fibro-granulomatous infiltration containing histiocytes and eosinophilic granular leukocytes (⁎). F Magnified view of histological section of infected ovary showing a fibro-granulomatous infiltrate surrounding clusters of eggs at different stages of development (mainly highly developed eggs, ➤). G High magnification (100×) view of a fully developed egg of H. persica (cross-sectional view) showing larva in-folded within the eggshell and a protruding polar plug at either end. H Less developed eggs with a nearly central nucleus (⁎) and thin eggshell layer and I fully developed eggs containing twisted larvae of H. persica cut on varying planes of section" figureDoi="http://doi.org/10.5281/zenodo.11552142" httpUri="https://zenodo.org/record/11552142/files/figure.png" pageId="8" pageNumber="9">Fig. 5A, B</figureCitation>
; more likely larval forms of 
<taxonomicName id="4C6EE9E1FF83D43EFA9723538B6DF8D5" authorityName="Dujardin" authorityYear="1845" class="Chromadorea" family="Anisakidae" genus="Anisakis" kingdom="Animalia" order="Rhabditida" pageId="8" pageNumber="9" phylum="Nematoda" rank="genus">
<emphasis id="B91A4E70FF83D43EFA9723538B6DF8D5" italics="true" pageId="8" pageNumber="9">Anisakis</emphasis>
</taxonomicName>
and less likely adult forms of 
<taxonomicName id="4C6EE9E1FF83D43EFB3223708D44F8D5" authorityName="Moravec" authorityYear="1987" box="[1225,1355,1849,1873]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="8" pageNumber="9" phylum="Nematoda" rank="genus">
<emphasis id="B91A4E70FF83D43EFB3223708D44F8D5" box="[1225,1355,1849,1873]" italics="true" pageId="8" pageNumber="9">Huffmanela</emphasis>
</taxonomicName>
as the females are usually extremely long, are rarely found encapsulated and are not much thicker than the eggs), all of which were enclosed in a fibro-granulomatous infiltrate. Ovigerous lamellae of the ovary were infected with nests of developing eggs from the nematode 
<taxonomicName id="4C6EE9E1FF82D43FFD6A25658B0EFEC7" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[657,769,300,323]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="persica">
<emphasis id="B91A4E70FF82D43FFD6A25658B0EFEC7" box="[657,769,300,323]" italics="true" pageId="9" pageNumber="10">H. persica</emphasis>
</taxonomicName>
. Te eggs were located within both clusters of immature and previtellogenic oocytes and the connective tissue of the lamellae and encased by a fibro-granulomatous infiltrate containing both histiocytes and eosinophilic granular leukocytes (
<figureCitation id="13558EE7FF82D43FFEC0258289ABFE67" box="[315,420,459,483]" captionStart="Fig" captionStartId="8.[167,198,1521,1541]" captionTargetBox="[290,1304,240,1508]" captionTargetId="figure-87@8.[284,1304,236,1508]" captionTargetPageId="8" captionText="Fig. 5 Photomicrographs from histological sections of infected tissues of a daggertooth pike conger eel. A, B Histological sections of the stomach infected by eggs of Huffmanela persica sp. nov. at various stages of development as well as degenerated encysted metazoans (⁎). C, D Sections of the tunica serosa of the stomach parasitized with completely developed eggs.E Sections of the ovarian lamellae infected by both clusters of immature (➔) and developing (➤) eggs, representing immature and previtellogenic oocytes embedded within a loose fibro-granulomatous infiltration containing histiocytes and eosinophilic granular leukocytes (⁎). F Magnified view of histological section of infected ovary showing a fibro-granulomatous infiltrate surrounding clusters of eggs at different stages of development (mainly highly developed eggs, ➤). G High magnification (100×) view of a fully developed egg of H. persica (cross-sectional view) showing larva in-folded within the eggshell and a protruding polar plug at either end. H Less developed eggs with a nearly central nucleus (⁎) and thin eggshell layer and I fully developed eggs containing twisted larvae of H. persica cut on varying planes of section" figureDoi="http://doi.org/10.5281/zenodo.11552142" httpUri="https://zenodo.org/record/11552142/files/figure.png" pageId="9" pageNumber="10">Fig. 5E, F</figureCitation>
).
</paragraph>
<paragraph id="8BD19262FF82D43FFF6C26448C22FCE7" blockId="9.[151,776,525,867]" lastBlockId="9.[813,1437,235,867]" pageId="9" pageNumber="10">
<emphasis id="B91A4E70FF82D43FFF6C26448A97FDA0" bold="true" box="[151,664,525,548]" pageId="9" pageNumber="10">Gene characterization and phylogenetic analysis</emphasis>
Sequencing of purified PCR products of 18S, 28S and ITS regions from 
<taxonomicName id="4C6EE9E1FF82D43FFE93260589D4FDE7" authority="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh, 2023" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[360,475,588,611]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="persica" status="sp. nov.">
<emphasis id="B91A4E70FF82D43FFE93260589D4FDE7" box="[360,475,588,611]" italics="true" pageId="9" pageNumber="10">H. persica</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A229F30BFF82D43FFE1E26058A32FDE7" box="[485,573,588,611]" pageId="9" pageNumber="10" rank="species">sp. nov.</taxonomicNameLabel>
resulted in DNA fragments with different lengths (855 bp, 1264 bp and 1127 bp, respectively). Te BLAST analysis revealed that 18S rDNA gene sequence of 
<taxonomicName id="4C6EE9E1FF82D43FFDD726E58AAEFD47" authority="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh, 2023" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[556,673,684,707]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="persica" status="sp. nov.">
<emphasis id="B91A4E70FF82D43FFDD726E58AAEFD47" box="[556,673,684,707]" italics="true" pageId="9" pageNumber="10">H. persica</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A229F30BFF82D43FFD5626E58B08FD47" box="[685,775,684,707]" pageId="9" pageNumber="10" rank="species">sp. nov.</taxonomicNameLabel>
shared 93–96% identity (ID) and 83–96% query coverage (QC) with 
<taxonomicName id="4C6EE9E1FF82D43FFE9126A28A41FC87" box="[362,590,747,771]" pageId="9" pageNumber="10">
<emphasis id="B91A4E70FF82D43FFE9126A289E0FC87" box="[362,495,747,771]" italics="true" pageId="9" pageNumber="10">Huffmanela</emphasis>
species
</taxonomicName>
collected from freshwater bony fish including 
<taxonomicName id="4C6EE9E1FF82D43FFE0427458AA2FCA7" box="[511,685,779,803]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" isUncertain="true" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="huffmani">
<emphasis id="B91A4E70FF82D43FFE0427458A16FCA7" box="[511,537,780,803]" italics="true" pageId="9" pageNumber="10">H.</emphasis>
cf. 
<emphasis id="B91A4E70FF82D43FFDBC27428AA2FCA7" box="[583,685,779,803]" italics="true" pageId="9" pageNumber="10">huffmani</emphasis>
</taxonomicName>
of Bullard et al., 2022 (ON838248, 93.12% ID and 96% QC), 
<taxonomicName id="4C6EE9E1FF82D43FFF6C2705892DFCE7" box="[151,290,843,867]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="huffmani">
<emphasis id="B91A4E70FF82D43FFF6C2705892DFCE7" box="[151,290,843,867]" italics="true" pageId="9" pageNumber="10">H. huffmani</emphasis>
</taxonomicName>
(ON838249, 94.62% ID and 88% QC), 
<taxonomicName id="4C6EE9E1FF82D43FFD1627058B9CFE87" box="[749,915,235,867]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="huffmani">
<emphasis id="B91A4E70FF82D43FFD1627058B9CFE87" box="[749,915,235,867]" italics="true" pageId="9" pageNumber="10">H. huffmani</emphasis>
</taxonomicName>
(ON838251, 96.08% ID and 83% QC) and 
<taxonomicName id="4C6EE9E1FF82D43FFA7924A58BB9FEA7" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" isUncertain="true" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="huffmani">
<emphasis id="B91A4E70FF82D43FFA7924A58D92FE87" box="[1410,1437,236,259]" italics="true" pageId="9" pageNumber="10">H.</emphasis>
cf. 
<emphasis id="B91A4E70FF82D43FFCAB25428BB9FEA7" box="[848,950,267,291]" italics="true" pageId="9" pageNumber="10">huffmani</emphasis>
</taxonomicName>
(ON838247, 95.80% ID and 83% QC) and 86–89% ID and 81–95% QC with those isolated from marine cartilaginous fish including 
<taxonomicName id="4C6EE9E1FF82D43FFB1D25028D93FEE7" box="[1254,1436,331,355]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="undetermined">
<emphasis id="B91A4E70FF82D43FFB1D25028D64FEE7" box="[1254,1387,331,355]" italics="true" pageId="9" pageNumber="10">Huffmanela</emphasis>
sp.
</taxonomicName>
(ON838247, 86.28% ID and 95% QC) and 
<taxonomicName id="4C6EE9E1FF82D43FFAEC25228CBBFE27" authority="Ruiz and Bullard, 2013" authorityName="Ruiz and Bullard" authorityYear="2013" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="markgracei">
<emphasis id="B91A4E70FF82D43FFAEC25228BA5FE27" italics="true" pageId="9" pageNumber="10">Huffmanela markgracei</emphasis>
Ruiz and Bullard, 2013
</taxonomicName>
(ON838250, 89.38% ID and 81% QC). According to the ML tree generated from 18S rDNA sequences (
<figureCitation id="13558EE7FF82D43FFB9425828CA0FE67" box="[1135,1199,459,483]" captionStart="Fig" captionStartId="9.[167,198,1847,1867]" captionTargetBox="[167,1419,929,1833]" captionTargetId="figure-351@9.[166,1421,928,1834]" captionTargetPageId="9" captionText="Fig. 6 Maximum likelihood (ML) phylogram reconstructed using the 18S rDNA dataset of the new species Huffmanela persica sp. nov. and other related species within the families Trichosomoididae,Trichinellidae,Trichuridae and Capillariidae. ML analysis was performed using the substitution model K2+G with 1000 bootstrap replications" figureDoi="http://doi.org/10.5281/zenodo.11552144" httpUri="https://zenodo.org/record/11552144/files/figure.png" pageId="9" pageNumber="10">Fig. 6</figureCitation>
), 
<taxonomicName id="4C6EE9E1FF82D43FFB3325858D35FE67" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[1224,1338,460,483]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="persica">
<emphasis id="B91A4E70FF82D43FFB3325858D35FE67" box="[1224,1338,460,483]" italics="true" pageId="9" pageNumber="10">H. persica</emphasis>
</taxonomicName>
was not grouped with previously reported species of 
<taxonomicName id="4C6EE9E1FF82D43FFA9925A28B8EFDA7" authorityName="Moravec" authorityYear="1987" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="genus">
<emphasis id="B91A4E70FF82D43FFA9925A28B8EFDA7" italics="true" pageId="9" pageNumber="10">Huffmanela</emphasis>
</taxonomicName>
. However, a sister relationship was recovered between the new species of marine origin with freshwater species of 
<taxonomicName id="4C6EE9E1FF82D43FFC0E26028C75FDE7" authorityName="Moravec" authorityYear="1987" box="[1013,1146,587,611]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="genus">
<emphasis id="B91A4E70FF82D43FFC0E26028C75FDE7" box="[1013,1146,587,611]" italics="true" pageId="9" pageNumber="10">Huffmanela</emphasis>
</taxonomicName>
(
<taxonomicName id="4C6EE9E1FF82D43FFB7426058D14FDE7" box="[1167,1307,587,611]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="huffmani">
<emphasis id="B91A4E70FF82D43FFB7426058D14FDE7" box="[1167,1307,587,611]" italics="true" pageId="9" pageNumber="10">H. huffmani</emphasis>
</taxonomicName>
and 
<taxonomicName id="4C6EE9E1FF82D43FFAA726058B9CFD07" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" isUncertain="true" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="huffmani">
<emphasis id="B91A4E70FF82D43FFAA726058D79FDE7" box="[1372,1398,588,611]" italics="true" pageId="9" pageNumber="10">H.</emphasis>
cf. 
<emphasis id="B91A4E70FF82D43FFCD626228B9CFD07" box="[813,915,619,643]" italics="true" pageId="9" pageNumber="10">huffmani</emphasis>
</taxonomicName>
), all of which are known to infect teleost fish. A sister group relationship was also found between 
<taxonomicName id="4C6EE9E1FF82D43FFCD626E28C04FD47" box="[813,1035,683,707]" pageId="9" pageNumber="10">
<emphasis id="B91A4E70FF82D43FFCD626E28BBDFD47" box="[813,946,683,707]" italics="true" pageId="9" pageNumber="10">Huffmanela</emphasis>
species
</taxonomicName>
infecting teleost fish (
<taxonomicName id="4C6EE9E1FF82D43FFAF626E58D99FD47" box="[1293,1430,683,707]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="huffmani">
<emphasis id="B91A4E70FF82D43FFAF626E58D99FD47" box="[1293,1430,683,707]" italics="true" pageId="9" pageNumber="10">H. huffmani</emphasis>
</taxonomicName>
, 
<taxonomicName id="4C6EE9E1FF82D43FFCD626858BEFFD67" box="[813,992,715,739]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" isUncertain="true" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="huffmani">
<emphasis id="B91A4E70FF82D43FFCD626858B48FD67" box="[813,839,716,739]" italics="true" pageId="9" pageNumber="10">H.</emphasis>
cf. 
<emphasis id="B91A4E70FF82D43FFC8126828BEFFD67" box="[890,992,715,739]" italics="true" pageId="9" pageNumber="10">huffmani</emphasis>
</taxonomicName>
and 
<taxonomicName id="4C6EE9E1FF82D43FFBDA26858C99FD67" authorityName="Ghanei-Motlagh &amp; Fast &amp; Groman &amp; Kumar &amp; Soliman &amp; El-Matbouli &amp; Saleh" authorityYear="2023" box="[1057,1174,716,739]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="persica">
<emphasis id="B91A4E70FF82D43FFBDA26858C99FD67" box="[1057,1174,716,739]" italics="true" pageId="9" pageNumber="10">H. persica</emphasis>
</taxonomicName>
) with those parasitizing elasmobranch fish (
<taxonomicName id="4C6EE9E1FF82D43FFBC126A58CD4FC87" authorityName="Ruiz and Bullard" authorityYear="2013" box="[1082,1243,747,771]" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="markgracei">
<emphasis id="B91A4E70FF82D43FFBC126A58CD4FC87" box="[1082,1243,747,771]" italics="true" pageId="9" pageNumber="10">H. markgracei</emphasis>
</taxonomicName>
and 
<taxonomicName id="4C6EE9E1FF82D43FFAEC26A28B41FCA7" class="Enoplea" family="Trichosomoididae" genus="Huffmanela" kingdom="Animalia" order="Trichinellida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="undetermined">
<emphasis id="B91A4E70FF82D43FFAEC26A28D93FC87" box="[1303,1436,747,771]" italics="true" pageId="9" pageNumber="10">Huffmanela</emphasis>
sp.
</taxonomicName>
). Phylogenetic analysis based on 18S rDNA dataset strongly corroborated monophyly of the subfamily 
<taxonomicName id="4C6EE9E1FF82D43FFCD627028BDDFCE7" box="[813,978,843,867]" pageId="9" pageNumber="10" rank="subFamily" subFamily="Huffmanelinae">Huffmanelinae</taxonomicName>
(
<figureCitation id="13558EE7FF82D43FFC1A27028C11FCE7" box="[993,1054,843,867]" captionStart="Fig" captionStartId="9.[167,198,1847,1867]" captionTargetBox="[167,1419,929,1833]" captionTargetId="figure-351@9.[166,1421,928,1834]" captionTargetPageId="9" captionText="Fig. 6 Maximum likelihood (ML) phylogram reconstructed using the 18S rDNA dataset of the new species Huffmanela persica sp. nov. and other related species within the families Trichosomoididae,Trichinellidae,Trichuridae and Capillariidae. ML analysis was performed using the substitution model K2+G with 1000 bootstrap replications" figureDoi="http://doi.org/10.5281/zenodo.11552144" httpUri="https://zenodo.org/record/11552144/files/figure.png" pageId="9" pageNumber="10">Fig. 6</figureCitation>
).
</paragraph>
</subSubSection>
</treatment>
</document>