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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.78.60063" ID-GBIF-Dataset="ed30d382-c89e-45e4-b917-cfa4c5625c8f" ID-PMC="PMC7884380" ID-Pensoft-Pub="1314-4049-78-1" ID-Pensoft-UUID="F83FEF511ABC587BBDC4A784A3A099D2" ID-PubMed="33613044" ModsDocID="1314-4049-78-1" checkinTime="1612984313627" checkinUser="pensoft" docAuthor="Keirnan, Elizabeth C., Tan, Yu Pei, Laurence, Matthew H., Mertin, Allison A., Liew, Edward C. Y., Summerell, Brett A. &amp; Shivas, Roger G." docDate="2021" docId="4045D173B7445746992446212C012F80" docLanguage="en" docName="MycoKeys 78: 1-20" docOrigin="MycoKeys 78" docPubDate="2021-02-08" docSource="http://dx.doi.org/10.3897/mycokeys.78.60063" docTitle="Nothophoma naiawu E. C. Keirnan, M. H. Laurence, R. G. Shivas &amp; Y. P. Tan 2021, sp. nov." docType="treatment" docVersion="3" id="F83FEF511ABC587BBDC4A784A3A099D2" lastPageNumber="1" masterDocId="F83FEF511ABC587BBDC4A784A3A099D2" masterDocTitle="Cryptic diversity found in Didymellaceae from Australian native legumes" masterLastPageNumber="20" masterPageNumber="1" pageNumber="1" updateTime="1668136617044" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Cryptic diversity found in Didymellaceae from Australian native legumes</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Keirnan, Elizabeth C.</mods:namePart>
<mods:affiliation>School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, SA 5005, Australia</mods:affiliation>
<mods:nameIdentifier type="email">elizabeth.keirnan@adelaide.edu.au</mods:nameIdentifier>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Tan, Yu Pei</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-4565-6148</mods:nameIdentifier>
<mods:affiliation>School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, SA 5005, Australia</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Laurence, Matthew H.</mods:namePart>
<mods:affiliation>Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, Mrs Macquaries Rd, Sydney, NSW 2000, Australia</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Mertin, Allison A.</mods:namePart>
<mods:affiliation>Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, Mrs Macquaries Rd, Sydney, NSW 2000, Australia</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Liew, Edward C. Y.</mods:namePart>
<mods:affiliation>Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, Mrs Macquaries Rd, Sydney, NSW 2000, Australia</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Summerell, Brett A.</mods:namePart>
<mods:affiliation>Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, Mrs Macquaries Rd, Sydney, NSW 2000, Australia</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Shivas, Roger G.</mods:namePart>
<mods:affiliation>Department of Agriculture and Fisheries, Ecosciences Precinct, Dutton Park, QLD 4102, Australia &amp; Centre for Crop Health, University of Southern Queensland, Toowoomba, QLD 4350, Australia</mods:affiliation>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
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<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2021</mods:date>
<mods:detail type="pubDate">
<mods:number>2021-02-08</mods:number>
</mods:detail>
<mods:detail type="volume">
<mods:number>78</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>1</mods:start>
<mods:end>20</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.78.60063</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.78.60063</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-78-1</mods:identifier>
<mods:identifier type="Pensoft-UUID">F83FEF511ABC587BBDC4A784A3A099D2</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="177318539" LSID="urn:lsid:plazi:treatment:4045D173B7445746992446212C012F80" httpUri="http://treatment.plazi.org/id/4045D173B7445746992446212C012F80" lastPageNumber="1" pageId="0" pageNumber="1">
<subSubSection pageId="0" pageNumber="1" type="nomenclature">
<paragraph pageId="0" pageNumber="1">
<taxonomicName LSID="4045D173-B744-5746-9924-46212C012F80" authority="E. C. Keirnan, M. H. Laurence, R. G. Shivas &amp; Y. P. Tan" authorityName="E. C. Keirnan, M. H. Laurence, R. G. Shivas &amp; Y. P. Tan" authorityYear="2021" class="Dothideomycetes" family="Didymellaceae" genus="Nothophoma" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nothophoma naiawu" order="Pleosporales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="naiawu" status="sp. nov.">Nothophoma naiawu E.C. Keirnan, M.H. Laurence, R.G. Shivas &amp; Y.P. Tan</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="1">sp. nov.</taxonomicNameLabel>
<figureCitation captionStart="Figure 5" captionStartId="F6" captionText="Figure 5. Nothophoma naiawu: a pin-prick leaf spots on Senna artemisioides b 14 - d old colonies top to bottom on PDA, MEA, OA (left, top to bottom) and lower surface (right) c upper surface d pycnidia on CLA e pycnidia f conidia. Scale bars: 300 µm (d, e); 10 µm (f)." figureDoi="10.3897/mycokeys.78.60063.figure5" httpUri="https://binary.pensoft.net/fig/506798" pageId="0" pageNumber="1">Fig. 5</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="materials_examined">
<paragraph pageId="0" pageNumber="1">Type.</paragraph>
<paragraph pageId="0" pageNumber="1">
Australia, South Australia, Blanchetown, from
<taxonomicName class="Magnoliopsida" family="Fabaceae" genus="Senna" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Senna artemisioides" order="Fabales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="species" species="artemisioides">
<emphasis italics="true" pageId="0" pageNumber="1">Senna artemisioides</emphasis>
</taxonomicName>
, 22 Oct. 2016,
<emphasis italics="true" pageId="0" pageNumber="1">E.C. Keirnan</emphasis>
, holotype BRIP 69583 (includes culture ex-type).
</paragraph>
<caption doi="10.3897/mycokeys.78.60063.figure5" httpUri="https://binary.pensoft.net/fig/506798" pageId="0" pageNumber="1" start="Figure 5" startId="F6">
<paragraph pageId="0" pageNumber="1">
<emphasis bold="true" pageId="0" pageNumber="1">Figure 5.</emphasis>
<taxonomicName authorityName="E. C. Keirnan, M. H. Laurence, R. G. Shivas &amp; Y. P. Tan" authorityYear="2021" class="Dothideomycetes" family="Didymellaceae" genus="Nothophoma" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nothophoma naiawu" order="Pleosporales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="naiawu">
<emphasis italics="true" pageId="0" pageNumber="1">Nothophoma naiawu</emphasis>
</taxonomicName>
:
<emphasis bold="true" pageId="0" pageNumber="1">a</emphasis>
pin-prick leaf spots on
<taxonomicName class="Magnoliopsida" family="Fabaceae" genus="Senna" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Senna artemisioides" order="Fabales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="species" species="artemisioides">
<emphasis italics="true" pageId="0" pageNumber="1">Senna artemisioides</emphasis>
</taxonomicName>
<emphasis bold="true" pageId="0" pageNumber="1">b</emphasis>
14-d old colonies top to bottom on PDA, MEA, OA (left, top to bottom) and lower surface (right)
<emphasis bold="true" pageId="0" pageNumber="1">c</emphasis>
upper surface
<emphasis bold="true" pageId="0" pageNumber="1">d</emphasis>
pycnidia on CLA
<emphasis bold="true" pageId="0" pageNumber="1">e</emphasis>
pycnidia
<emphasis bold="true" pageId="0" pageNumber="1">f</emphasis>
conidia. Scale bars: 300
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="1">d, e</emphasis>
); 10
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="1">f)</emphasis>
.
</paragraph>
</caption>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="description">
<paragraph pageId="0" pageNumber="1">Description.</paragraph>
<paragraph pageId="0" pageNumber="1">
<emphasis italics="true" pageId="0" pageNumber="1">Colonies</emphasis>
on OA, 21-25 mm diam. after 7 d, flat with scant aerial mycelia, rosy vinaceous, dark at centre; reverse rosy buff, dark at centre, with a few dark radiating fissures; on MEA, 27-30 mm after 7 d, margin entire, flat, with sparse aerial mycelium towards centre rosy vinaceous; reverse peach, darker at centre; on PDA, 27-30 mm after 7 d, margin entire, flat felty, rosy buff; reverse peach, dark at centre.
<emphasis italics="true" pageId="0" pageNumber="1">NaOH spot test</emphasis>
: slightly yellow.
<emphasis italics="true" pageId="0" pageNumber="1">Conidiomata</emphasis>
pycnidial, globose to subglobose, 200-300
<normalizedToken originalValue="μm">μm</normalizedToken>
diam., pale brown becoming black, semi-immersed, confluent on MEA, glabrous, non-papillate; ostiole c. 25
<normalizedToken originalValue="μm">μm</normalizedToken>
diam.; pycnidial wall composed of textura globulosa, pale brown, cells 5-8
<normalizedToken originalValue="μm">μm</normalizedToken>
diam..
<emphasis italics="true" pageId="0" pageNumber="1">Conidiogenous</emphasis>
cells phialidic, cylindrical, very thin-walled, hyaline.
<emphasis italics="true" pageId="0" pageNumber="1">Conidia</emphasis>
aseptate or 1-septate, 8-12
<normalizedToken originalValue="×">x</normalizedToken>
4-6
<normalizedToken originalValue="μm">μm</normalizedToken>
, cylindrical to narrow ellipsoidal, pale yellow.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="etymology">
<paragraph pageId="0" pageNumber="1">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="1">
A variation of the Indigenous Australian Ngayawang
<normalizedToken originalValue="peoples">people's</normalizedToken>
language group, who lived in the Murray River region of South Australia, which includes Blanchetown, the locality where this specimen was collected.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="notes">
<paragraph pageId="0" pageNumber="1">Notes.</paragraph>
<paragraph pageId="0" pageNumber="1">
<taxonomicName authorityName="E. C. Keirnan, M. H. Laurence, R. G. Shivas &amp; Y. P. Tan" authorityYear="2021" class="Dothideomycetes" family="Didymellaceae" genus="Nothophoma" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nothophoma naiawu" order="Pleosporales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="naiawu">
<emphasis italics="true" pageId="0" pageNumber="1">Nothophoma naiawu</emphasis>
</taxonomicName>
is phylogenetically close to
<taxonomicName lsidName="No. eucalyptigena" pageId="0" pageNumber="1" rank="species" species="eucalyptigena">
<emphasis italics="true" pageId="0" pageNumber="1">No. eucalyptigena</emphasis>
</taxonomicName>
and
<taxonomicName lsidName="No. infuscata" pageId="0" pageNumber="1" rank="species" species="infuscata">
<emphasis italics="true" pageId="0" pageNumber="1">No. infuscata</emphasis>
</taxonomicName>
(Fig.
<figureCitation captionStart="Figure 2" captionStartId="F3" captionText="Figure 2. Epicoccum djirangnandiri: a leaf lesions on Swainsona galegifolia b 14 - d old colonies on PDA, MEA, OA (left, top to bottom) and lower surface (right) c upper surface d pycnidia on CLA e conidia. Scale bars: 200 µm (d); 7 µm (e)." figureDoi="10.3897/mycokeys.78.60063.figure2" httpUri="https://binary.pensoft.net/fig/506795" pageId="0" pageNumber="1">2</figureCitation>
).
<taxonomicName authorityName="E. C. Keirnan, M. H. Laurence, R. G. Shivas &amp; Y. P. Tan" authorityYear="2021" class="Dothideomycetes" family="Didymellaceae" genus="Nothophoma" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nothophoma naiawu" order="Pleosporales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="naiawu">
<emphasis italics="true" pageId="0" pageNumber="1">Nothophoma naiawu</emphasis>
</taxonomicName>
is easily distinguished from
<taxonomicName lsidName="No. eucalyptigena" pageId="0" pageNumber="1" rank="species" species="eucalyptigena">
<emphasis italics="true" pageId="0" pageNumber="1">No. eucalyptigena</emphasis>
</taxonomicName>
and
<taxonomicName lsidName="No. infuscata" pageId="0" pageNumber="1" rank="species" species="infuscata">
<emphasis italics="true" pageId="0" pageNumber="1">No. infuscata</emphasis>
</taxonomicName>
by the ITS region (98 % identity to both) and the
<emphasis italics="true" pageId="0" pageNumber="1">rpb</emphasis>
2 locus (95%, and 94% identity, respectively).
<taxonomicName authorityName="L.W.Hou, L.Cai &amp; Crous" authorityYear="2020" class="Dothideomycetes" family="Didymellaceae" genus="Nothophoma" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nothophoma infuscata" order="Pleosporales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="infuscata">
<emphasis italics="true" pageId="0" pageNumber="1">Nothophoma infuscata</emphasis>
</taxonomicName>
produce a pale red discolouration in response to
<emphasis italics="true" pageId="0" pageNumber="1">NaOH spot test</emphasis>
on MEA media, which is distinct from the slightly yellow response by
<taxonomicName lsidName="No. naiawu" pageId="0" pageNumber="1" rank="species" species="naiawu">
<emphasis italics="true" pageId="0" pageNumber="1">No. naiawu</emphasis>
</taxonomicName>
.
</paragraph>
</subSubSection>
</treatment>
</document>