Cryptic diversity found in Didymellaceae from Australian native legumes Author Keirnan, Elizabeth C. School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, SA 5005, Australia elizabeth.keirnan@adelaide.edu.au Author Tan, Yu Pei https://orcid.org/0000-0002-4565-6148 School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, SA 5005, Australia Author Laurence, Matthew H. Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, Mrs Macquaries Rd, Sydney, NSW 2000, Australia Author Mertin, Allison A. Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, Mrs Macquaries Rd, Sydney, NSW 2000, Australia Author Liew, Edward C. Y. Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, Mrs Macquaries Rd, Sydney, NSW 2000, Australia Author Summerell, Brett A. Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, Mrs Macquaries Rd, Sydney, NSW 2000, Australia Author Shivas, Roger G. Department of Agriculture and Fisheries, Ecosciences Precinct, Dutton Park, QLD 4102, Australia & Centre for Crop Health, University of Southern Queensland, Toowoomba, QLD 4350, Australia text MycoKeys 2021 2021-02-08 78 1 20 http://dx.doi.org/10.3897/mycokeys.78.60063 journal article http://dx.doi.org/10.3897/mycokeys.78.60063 1314-4049-78-1 F83FEF511ABC587BBDC4A784A3A099D2 Nothophoma naiawu E.C. Keirnan, M.H. Laurence, R.G. Shivas & Y.P. Tan sp. nov. Fig. 5 Type. Australia, South Australia, Blanchetown, from Senna artemisioides , 22 Oct. 2016, E.C. Keirnan , holotype BRIP 69583 (includes culture ex-type). Figure 5. Nothophoma naiawu : a pin-prick leaf spots on Senna artemisioides b 14-d old colonies top to bottom on PDA, MEA, OA (left, top to bottom) and lower surface (right) c upper surface d pycnidia on CLA e pycnidia f conidia. Scale bars: 300 µm ( d, e ); 10 µm ( f) . Description. Colonies on OA, 21-25 mm diam. after 7 d, flat with scant aerial mycelia, rosy vinaceous, dark at centre; reverse rosy buff, dark at centre, with a few dark radiating fissures; on MEA, 27-30 mm after 7 d, margin entire, flat, with sparse aerial mycelium towards centre rosy vinaceous; reverse peach, darker at centre; on PDA, 27-30 mm after 7 d, margin entire, flat felty, rosy buff; reverse peach, dark at centre. NaOH spot test : slightly yellow. Conidiomata pycnidial, globose to subglobose, 200-300 μm diam., pale brown becoming black, semi-immersed, confluent on MEA, glabrous, non-papillate; ostiole c. 25 μm diam.; pycnidial wall composed of textura globulosa, pale brown, cells 5-8 μm diam.. Conidiogenous cells phialidic, cylindrical, very thin-walled, hyaline. Conidia aseptate or 1-septate, 8-12 x 4-6 μm , cylindrical to narrow ellipsoidal, pale yellow. Etymology. A variation of the Indigenous Australian Ngayawang people's language group, who lived in the Murray River region of South Australia, which includes Blanchetown, the locality where this specimen was collected. Notes. Nothophoma naiawu is phylogenetically close to No. eucalyptigena and No. infuscata (Fig. 2 ). Nothophoma naiawu is easily distinguished from No. eucalyptigena and No. infuscata by the ITS region (98 % identity to both) and the rpb 2 locus (95%, and 94% identity, respectively). Nothophoma infuscata produce a pale red discolouration in response to NaOH spot test on MEA media, which is distinct from the slightly yellow response by No. naiawu .