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<document id="76B68A1E6ECF30089A344324A83D5231" ID-DOI="10.1051/parasite/2021072" ID-ISSN="1776-1042" ID-PMC="PMC8577333" ID-PubMed="34751646" ID-Zenodo-Dep="12628725" ID-ZooBank="3029BD6E-891B-4635-AFDC-AAF5719A4877" IM.bibliography_approvedBy="valdenar" IM.illustrations_approvedBy="valdenar" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="valdenar" checkinTime="1719954530783" checkinUser="felipe" docAuthor="Cutmore, Scott C. &amp; Cribb, Thomas H." docDate="2021" docId="039B210FFFDEFF9A0951F9EC960AFA21" docLanguage="en" docName="Parasite.28.76.pdf" docOrigin="Parasite (Paris, France) 28 (76)" docSource="http://dx.doi.org/10.1051/parasite/2021072" docStyle="DocumentStyle:A40D95A4C09C797DBB807E438D2BF437.2:Parasite.2013-.journal_article" docStyleId="A40D95A4C09C797DBB807E438D2BF437" docStyleName="Parasite.2013-.journal_article" docStyleVersion="2" docTitle="Holocentricola undefined-A" docType="treatment" docVersion="3" lastPageNumber="11" masterDocId="FFA25977FFD4FF900920FF8D933AFFDA" masterDocTitle="A new order of fishes as hosts of blood flukes (Aporocotylidae); description of a new genus and three new species infecting squirrelfishes (Holocentriformes, Holocentridae) on the Great Barrier Reef" masterLastPageNumber="17" masterPageNumber="1" pageNumber="11" updateTime="1721745722827" updateUser="valdenar" zenodo-license-document="CC-BY-4.0">
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<mods:title id="381810245A31972A433DDD7BE10543C3">A new order of fishes as hosts of blood flukes (Aporocotylidae); description of a new genus and three new species infecting squirrelfishes (Holocentriformes, Holocentridae) on the Great Barrier Reef</mods:title>
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<mods:namePart id="65322249947D8D4B3C4A43392956325A">Cutmore, Scott C.</mods:namePart>
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<mods:namePart id="7D79AA7CF3A0415169099BEE37EDCA48">Cribb, Thomas H.</mods:namePart>
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<treatment id="039B210FFFDEFF9A0951F9EC960AFA21" LSID="urn:lsid:plazi:treatment:039B210FFFDEFF9A0951F9EC960AFA21" httpUri="http://treatment.plazi.org/id/039B210FFFDEFF9A0951F9EC960AFA21" lastPageNumber="11" pageId="10" pageNumber="11">
<subSubSection id="C328C392FFDEFF9A0951F9EC924CF9A3" box="[113,374,1632,1657]" pageId="10" pageNumber="11" type="nomenclature">
<paragraph id="8B8D9019FFDEFF9A0951F9EC924CF9A3" blockId="10.[113,374,1632,1657]" box="[113,374,1632,1657]" pageId="10" pageNumber="11">
<heading id="D0C52775FFDEFF9A0951F9EC924CF9A3" box="[113,374,1632,1657]" fontSize="9" level="2" pageId="10" pageNumber="11" reason="4">
<taxonomicName id="4C32EB9AFFDEFF9A0951F9EC924CF9A3" box="[113,374,1632,1657]" class="Trematoda" family="Aporocotylidae" genus="Holocentricola" kingdom="Animalia" order="Diplostomida" pageId="10" pageNumber="11" phylum="Platyhelminthes" rank="species" species="undefined-A">
<emphasis id="B9464C0BFFDEFF9A0951F9EC924CF9A3" bold="true" box="[113,374,1632,1657]" pageId="10" pageNumber="11">
<emphasis id="B9464C0BFFDEFF9A0951F9EC9210F9A3" bold="true" box="[113,298,1633,1657]" italics="true" pageId="10" pageNumber="11">Holocentricola</emphasis>
sp. A
</emphasis>
</taxonomicName>
</heading>
</paragraph>
</subSubSection>
<subSubSection id="C328C392FFDEFF9A09B9F91592E7F917" pageId="10" pageNumber="11" type="etymology">
<paragraph id="8B8D9019FFDEFF9A09B9F91592E7F917" blockId="10.[113,768,1687,2046]" pageId="10" pageNumber="11">
<emphasis id="B9464C0BFFDEFF9A09B9F91593F3F975" box="[153,201,1688,1711]" italics="true" pageId="10" pageNumber="11">Host</emphasis>
:
<taxonomicName id="4C32EB9AFFDEFF9A09C0F915917BF974" authority="(Forsskal)" baseAuthorityName="Forsskal" baseAuthorityYear="1775" box="[224,577,1687,1711]" family="Holocentridae" genus="Sargocentron" kingdom="Animalia" order="Beryciformes" pageId="10" pageNumber="11" phylum="Chordata" rank="species" species="rubrum">
<emphasis id="B9464C0BFFDEFF9A09C0F91592F3F975" box="[224,457,1688,1711]" italics="true" pageId="10" pageNumber="11">Sargocentron rubrum</emphasis>
(Forsskål)
</taxonomicName>
, Red squirrelfish (
<taxonomicName id="4C32EB9AFFDEFF9A0958F93B920BF917" box="[120,305,1718,1741]" order="Holocentriformes" pageId="10" pageNumber="14" rank="order">Holocentriformes</taxonomicName>
:
<taxonomicName id="4C32EB9AFFDEFF9A081DF93B92E9F917" box="[317,467,1718,1741]" family="Holocentridae" kingdom="Animalia" order="Beryciformes" pageId="10" pageNumber="11" phylum="Chordata" rank="family">Holocentridae</taxonomicName>
).
</paragraph>
</subSubSection>
<subSubSection id="C328C392FFDEFF9A09B9F958960AFA21" pageId="10" pageNumber="11" type="description">
<paragraph id="8B8D9019FFDEFF9A09B9F958929DF8D0" blockId="10.[113,768,1687,2046]" pageId="10" pageNumber="11">
<emphasis id="B9464C0BFFDEFF9A09B9F95893D6F936" box="[153,236,1749,1772]" italics="true" pageId="10" pageNumber="11">Locality</emphasis>
: Off Heron Island (23
<emphasis id="B9464C0BFFDEFF9A08F1F95992E1F936" bold="true" box="[465,475,1748,1772]" italics="true" pageId="10" pageNumber="11">°</emphasis>
<superScript id="7C473D51FFDEFF9A08C2F959913BF93B" attach="left" box="[482,513,1745,1771]" fontSize="6" pageId="10" pageNumber="11">270</superScript>
S, 151
<emphasis id="B9464C0BFFDEFF9A0B6BF959916FF936" bold="true" box="[587,597,1748,1772]" italics="true" pageId="10" pageNumber="11">°</emphasis>
<superScript id="7C473D51FFDEFF9A0B7CF9599140F93B" attach="left" box="[604,634,1745,1771]" fontSize="6" pageId="10" pageNumber="11">550</superScript>
E), southern Great Barrier Reef,
<collectingCountry id="F325D089FFDEFF9A0862F97E9298F8D0" box="[322,418,1779,1802]" name="Australia" pageId="10" pageNumber="11">Australia</collectingCountry>
.
</paragraph>
<paragraph id="8B8D9019FFDEFF9A09B9F89F93E7F89D" blockId="10.[113,768,1687,2046]" pageId="10" pageNumber="11">
<emphasis id="B9464C0BFFDEFF9A09B9F89F922CF8F3" box="[153,278,1810,1833]" italics="true" pageId="10" pageNumber="11">Site in host</emphasis>
: Branchial arteries, vessels of liver, wash of head split.
</paragraph>
<paragraph id="8B8D9019FFDEFF9A09B9F8C29276F85E" blockId="10.[113,768,1687,2046]" pageId="10" pageNumber="11">
<emphasis id="B9464C0BFFDEFF9A09B9F8C29236F8BC" box="[153,268,1871,1894]" italics="true" pageId="10" pageNumber="11">Prevalence</emphasis>
: 3 of 17 Heron Island (adult worms in three); 0 of 2 Lizard Island.
</paragraph>
<paragraph id="8B8D9019FFDEFF9A09B9F80193F4F81B" blockId="10.[113,768,1687,2046]" pageId="10" pageNumber="11">
<emphasis id="B9464C0BFFDEFF9A09B9F80193CAF879" box="[153,240,1932,1955]" italics="true" pageId="10" pageNumber="11">Intensity</emphasis>
: 1 worm per fish, when adult worms were detected.
</paragraph>
<paragraph id="8B8D9019FFDEFF9A09B9F84492BFF824" blockId="10.[113,768,1687,2046]" pageId="10" pageNumber="11">
<emphasis id="B9464C0BFFDEFF9A09B9F8449276F83A" box="[153,332,1993,2016]" italics="true" pageId="10" pageNumber="11">Voucher material</emphasis>
: Two voucher specimens (
<collectionCode id="ED2308DCFFDEFF9A0B40F84491B6F83A" box="[608,652,1993,2016]" country="Australia" lsid="urn:lsid:biocol.org:col:35019" name="Queensland Museum" pageId="10" pageNumber="11" type="Museum">QM</collectionCode>
G239129 30), both hologenophores.
</paragraph>
<paragraph id="8B8D9019FFDEFF9A0A75FD3F9725FC99" blockId="10.[813,1467,690,835]" pageId="10" pageNumber="11">
<emphasis id="B9464C0BFFDEFF9A0A75FD3F97ADFD13" box="[853,1175,690,713]" italics="true" pageId="10" pageNumber="11">Representative DNA sequences</emphasis>
: Partial
<emphasis id="B9464C0BFFDEFF9A0DCEFD3F9628FD13" box="[1262,1298,690,713]" italics="true" pageId="10" pageNumber="11">cox</emphasis>
1 mtDNA, three identical sequences (one submitted to GenBank, OK421329); ITS2 rDNA, one sequence (submitted to GenBank, OK422502); partial 28S rDNA, one sequence (submitted to GenBank, OK422505).
</paragraph>
<paragraph id="8B8D9019FFDEFF9A0A0DFCEA90B6FCA1" blockId="10.[813,908,871,891]" box="[813,908,871,891]" pageId="10" pageNumber="11">
<emphasis id="B9464C0BFFDEFF9A0A0DFCEA90B6FCA1" bold="true" box="[813,908,871,891]" pageId="10" pageNumber="11">Remarks</emphasis>
</paragraph>
<paragraph id="8B8D9019FFDEFF9A0A75FC2D960AFA21" blockId="10.[813,1468,928,1531]" pageId="10" pageNumber="11">
This putative, undescribed species was found co-infecting, with
<taxonomicName id="4C32EB9AFFDEFF9A0A43FC329083FC0C" authorityName="Cutmore &amp; Cribb" authorityYear="2021" box="[867,953,959,982]" class="Trematoda" family="Aporocotylidae" genus="Holocentricola" kingdom="Animalia" order="Diplostomida" pageId="10" pageNumber="11" phylum="Platyhelminthes" rank="species" species="rufus">
<emphasis id="B9464C0BFFDEFF9A0A43FC329083FC0C" box="[867,953,959,982]" italics="true" pageId="10" pageNumber="11">H. rufus</emphasis>
</taxonomicName>
, three individuals of
<taxonomicName id="4C32EB9AFFDEFF9A0DBEFC329632FC0C" baseAuthorityName="Forsskal" baseAuthorityYear="1775" box="[1182,1288,959,982]" family="Holocentridae" genus="Sargocentron" kingdom="Animalia" order="Beryciformes" pageId="10" pageNumber="11" phylum="Chordata" rank="species" species="rubrum">
<emphasis id="B9464C0BFFDEFF9A0DBEFC329632FC0C" box="[1182,1288,959,982]" italics="true" pageId="10" pageNumber="11">S. rubrum</emphasis>
</taxonomicName>
at Heron Island; only
<specimenCount id="9D345B90FFDEFF9A0A47FC50972BFC2E" box="[871,1041,989,1012]" count="3" pageId="10" pageNumber="11" type="generic">three specimens</specimenCount>
were collected, and just two hologenophores were available for morphological analysis. This species is genetically distinct from but sister to
<taxonomicName id="4C32EB9AFFDEFF9A0DE7FB979626FBEB" authorityName="Cutmore &amp; Cribb" authorityYear="2021" box="[1223,1308,1050,1073]" class="Trematoda" family="Aporocotylidae" genus="Holocentricola" kingdom="Animalia" order="Diplostomida" pageId="10" pageNumber="11" phylum="Platyhelminthes" rank="species" species="rufus">
<emphasis id="B9464C0BFFDEFF9A0DE7FB979626FBEB" box="[1223,1308,1050,1073]" italics="true" pageId="10" pageNumber="11">H. rufus</emphasis>
</taxonomicName>
in all phylogenetic analyses; despite the shared host and close phylogenetic affinity, the two species are clearly distinct morphologically. Specimens of
<taxonomicName id="4C32EB9AFFDEFF9A0A9EFBFB97AFFB57" box="[958,1173,1142,1165]" class="Trematoda" family="Aporocotylidae" genus="Holocentricola" kingdom="Animalia" order="Diplostomida" pageId="10" pageNumber="11" phylum="Platyhelminthes" rank="species" species="undefined-A">
<emphasis id="B9464C0BFFDEFF9A0A9EFBFB9763FB57" box="[958,1113,1142,1165]" italics="true" pageId="10" pageNumber="11">Holocentricola</emphasis>
sp. A
</taxonomicName>
are much smaller than those representing
<taxonomicName id="4C32EB9AFFDEFF9A0A9AFB18972CFB76" authorityName="Cutmore &amp; Cribb" authorityYear="2021" box="[954,1046,1173,1196]" class="Trematoda" family="Aporocotylidae" genus="Holocentricola" kingdom="Animalia" order="Diplostomida" pageId="10" pageNumber="11" phylum="Platyhelminthes" rank="species" species="rufus">
<emphasis id="B9464C0BFFDEFF9A0A9AFB18972CFB76" box="[954,1046,1173,1196]" italics="true" pageId="10" pageNumber="11">H. rufus</emphasis>
</taxonomicName>
, with the two hologenophores being narrower (107130
<emphasis id="B9464C0BFFDEFF9A0ADBFB3E972DFB10" box="[1019,1047,1203,1226]" italics="true" pageId="10" pageNumber="11">vs.</emphasis>
142222), having a shorter testis length (70
<emphasis id="B9464C0BFFDEFF9A0A77FB5F9049FB33" box="[855,883,1234,1257]" italics="true" pageId="10" pageNumber="11">vs.</emphasis>
100233), post-testicular space (203210
<emphasis id="B9464C0BFFDEFF9A0C0EFB5F9670FB33" box="[1326,1354,1234,1257]" italics="true" pageId="10" pageNumber="11">vs.</emphasis>
250351), post-ovarian space (153181
<emphasis id="B9464C0BFFDEFF9A0D46FB7D97B8FADD" box="[1126,1154,1264,1287]" italics="true" pageId="10" pageNumber="11">vs.</emphasis>
216301), post-oötype space (5574
<emphasis id="B9464C0BFFDEFF9A0AA1FA8290A7FAFC" box="[897,925,1295,1318]" italics="true" pageId="10" pageNumber="11">vs.</emphasis>
81138), post-male genital pore space (5556
<emphasis id="B9464C0BFFDEFF9A0CBFFA829681FAFC" box="[1439,1467,1295,1318]" italics="true" pageId="10" pageNumber="11">vs.</emphasis>
6696), and post-female genital pore space (5574
<emphasis id="B9464C0BFFDEFF9A0CBFFAA09681FA9E" box="[1439,1467,1325,1348]" italics="true" pageId="10" pageNumber="11">vs.</emphasis>
81138). Specimens of
<taxonomicName id="4C32EB9AFFDEFF9A0D0BFAC19633FAB9" box="[1067,1289,1356,1379]" class="Trematoda" family="Aporocotylidae" genus="Holocentricola" kingdom="Animalia" order="Diplostomida" pageId="10" pageNumber="11" phylum="Platyhelminthes" rank="species" species="undefined-A">
<emphasis id="B9464C0BFFDEFF9A0D0BFAC197FCFAB9" box="[1067,1222,1356,1379]" italics="true" pageId="10" pageNumber="11">Holocentricola</emphasis>
sp. A
</taxonomicName>
also have fewer spines per row than
<taxonomicName id="4C32EB9AFFDEFF9A0ADEFAE7976EFA5B" authorityName="Cutmore &amp; Cribb" authorityYear="2021" box="[1022,1108,1386,1409]" class="Trematoda" family="Aporocotylidae" genus="Holocentricola" kingdom="Animalia" order="Diplostomida" pageId="10" pageNumber="11" phylum="Platyhelminthes" rank="species" species="rufus">
<emphasis id="B9464C0BFFDEFF9A0ADEFAE7976EFA5B" box="[1022,1108,1386,1409]" italics="true" pageId="10" pageNumber="11">H. rufus</emphasis>
</taxonomicName>
(7
<emphasis id="B9464C0BFFDEFF9A0D57FAE797A9FA5B" box="[1143,1171,1386,1409]" italics="true" pageId="10" pageNumber="11">vs.</emphasis>
89), smaller spines (6 long
<emphasis id="B9464C0BFFDEFF9A0A0DFA049073FA7A" box="[813,841,1417,1440]" italics="true" pageId="10" pageNumber="11">vs.</emphasis>
78 long) and shorter spine rows (811 wide
<emphasis id="B9464C0BFFDEFF9A0C65FA04965BFA7A" box="[1349,1377,1417,1440]" italics="true" pageId="10" pageNumber="11">vs.</emphasis>
1122). The two hologenophore slides have been lodged as voucher specimens in the QM in the hope that future collecting in the region will enable the description of this species.
</paragraph>
</subSubSection>
</treatment>
</document>