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<document id="7CC8AAC498B68971960102AD376FBD2F" ID-DOI="10.5281/zenodo.10396362" ID-ISSN="1942-1354" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1707168658757" checkinUser="felipe" docAuthor="Zhang, Jing, Cong, Qian &amp; Grishin, Nick V." docDate="2023" docId="03810139FFCCBB43C0CAFE19E709B775" docLanguage="en" docName="InsectaMundi.2023.1026.1-115.pdf" docOrigin="Insecta Mundi 2023 (26)" docSource="http://dx.doi.org/10.5281/zenodo.10396362" docStyle="DocumentStyle:F9976E9B7F8A094D890004515A7A0592.3:InsectaMundi.2020-.journal_article" docStyleId="F9976E9B7F8A094D890004515A7A0592" docStyleName="InsectaMundi.2020-.journal_article" docStyleVersion="3" docTitle="Pythonides lera Zhang &amp; Cong &amp; Grishin 2023, new species" docType="treatment" docVersion="1" lastPageNumber="18" masterDocId="FFB87941FFDFBB50C00AFF56E355B262" masterDocTitle="Supplementary Materials and Appendix" masterLastPageNumber="115" masterPageNumber="1" pageNumber="18" updateTime="1707169288913" updateUser="GgImagineBatch" zenodo-license-document="CC-BY-NC-3.0">
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<mods:title id="CC25FBFE2E33195BA643D42498327A59">Supplementary Materials and Appendix</mods:title>
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<mods:name id="7869B575F0A76BE23CD30CC0E2CD968D" type="personal">
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<mods:namePart id="21BD1B12C1F3C77A201FF2A062792A98">Zhang, Jing</mods:namePart>
<mods:affiliation id="EF7623185B01D11D56040E278C42FB53">McDermott Center for Human Growth and Development and Department of Biophysics University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 8816 USA</mods:affiliation>
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<mods:namePart id="4A5B857AFDDB32D4EA38AF54DB92078A">Cong, Qian</mods:namePart>
<mods:affiliation id="64BC91485588A2430EA399FA3B00A41E">McDermott Center for Human Growth and Development and Department of Biophysics University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 8816 USA</mods:affiliation>
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<mods:namePart id="DEC2C9E56A73CDDF2256A0BBFDF9A6A8">Grishin, Nick V.</mods:namePart>
<mods:affiliation id="C04DF2D76812255B228D0CC72CC63ED0">Departments of Biophysics and Biochemistry University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 9050 USA</mods:affiliation>
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<mods:title id="8C37FEAF39EC94D639B02F97081F2A4F">Insecta Mundi</mods:title>
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<mods:date id="9B23C383C23EBBCF9B707F6290A39A36">2023</mods:date>
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<emphasis id="B95C6C3DFFCCBB43C0CAFE19E1C4B30E" bold="true" box="[192,657,335,364]" pageId="19" pageNumber="18">
<taxonomicName id="4C28CBACFFCCBB43C0CAFE19E2A5B30E" authority="Grishin" authorityName="Zhang &amp; Cong &amp; Grishin" authorityYear="2023" box="[192,496,335,364]" class="Insecta" family="Hesperiidae" genus="Pythonides" kingdom="Animalia" order="Lepidoptera" pageId="19" pageNumber="18" phylum="Arthropoda" rank="species" species="lera" status="sp. nov.">
<emphasis id="B95C6C3DFFCCBB43C0CAFE19E2D1B30E" bold="true" box="[192,388,335,364]" italics="true" pageId="19" pageNumber="18">Pythonides lera</emphasis>
Grishin
</taxonomicName>
,
<taxonomicNameLabel id="A26FD146FFCCBB43C1F6FE06E1C4B30E" box="[508,657,335,364]" pageId="19" pageNumber="18" rank="species">new species</taxonomicNameLabel>
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https://zoobank.org/
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<subSubSection id="C332E3A4FFCCBB43C0CAFEC3E157B3CD" box="[192,514,405,432]" pageId="19" pageNumber="18" type="description">
<paragraph id="8B97B02FFFCCBB43C0CAFEC3E157B3CD" blockId="19.[192,927,335,432]" box="[192,514,405,432]" pageId="19" pageNumber="18">
(
<figureCitation id="1313ACAAFFCCBB43C0C2FEC3E252B3CD" box="[200,263,405,431]" captionStart="Figure 2" captionStartId="20.[192,261,1419,1443]" captionTargetBox="[193,1440,268,1379]" captionTargetId="figure-157@20.[192,1440,266,1382]" captionTargetPageId="20" captionText="Figure 2. Phylogenetic trees of Achlyodini inferred from protein-coding regions in a) the Z chromosome and b) the mitochondrial genome (in segments). See Fig. 1 legend for other notations." pageId="19" pageNumber="18">Fig. 2</figureCitation>
part, 3536, 248249)
</paragraph>
</subSubSection>
<subSubSection id="C332E3A4FFCCBB43C0CAFEE8E7D8B184" pageId="19" pageNumber="18" type="diagnosis">
<paragraph id="8B97B02FFFCCBB43C0CAFEE8E247B16B" blockId="19.[191,1441,446,777]" pageId="19" pageNumber="18">
<emphasis id="B95C6C3DFFCCBB43C0CAFEE8E2BDB3BA" bold="true" box="[192,488,446,473]" pageId="19" pageNumber="18">Definition and diagnosis.</emphasis>
A specimen from
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identified as
<taxonomicName id="4C28CBACFFCCBB43C38AFEE9E65DB3BB" authority="(Hewitson, 1868)" baseAuthorityName="Hewitson" baseAuthorityYear="1868" box="[896,1288,447,473]" class="Insecta" family="Hesperiidae" genus="Pythonides" kingdom="Animalia" order="Lepidoptera" pageId="19" pageNumber="18" phylum="Arthropoda" rank="species" species="lerina">
<emphasis id="B95C6C3DFFCCBB43C38AFEE9E76AB3BB" box="[896,1087,447,473]" italics="true" pageId="19" pageNumber="18">Pythonides lerina</emphasis>
(
<bibRefCitation id="EFB9CDDEFFCCBB43C444FEE9E655B3BB" author="Hewitson WC" box="[1102,1280,447,473]" pageId="19" pageNumber="18" pagination="25 - 56" refId="ref77559" refString="Hewitson WC. 1868. Descriptions of one hundred new species of Hesperiidae 2. John Van Voorst; London. p. 25 - 56." type="book chapter" year="1868">Hewitson, 1868</bibRefCitation>
)
</taxonomicName>
(
<typeStatus id="54930E8DFFCCBB43C51CFEE9E61DB3BB" box="[1302,1352,447,473]" pageId="19" pageNumber="18">type</typeStatus>
locality in
<collectingCountry id="F33FF0BFFFCCBB43C0D7FEB6E2D1B398" box="[221,388,480,506]" name="French Guiana" pageId="19" pageNumber="18">French Guiana</collectingCountry>
and
<collectingCountry id="F33FF0BFFFCCBB43C1B1FEB6E155B398" box="[443,512,480,506]" name="Brazil" pageId="19" pageNumber="18">Brazil</collectingCountry>
: Para) is genetically differentiated from it (COI barcodes differ by 5% (33 bp)) (
<figureCitation id="1313ACAAFFCCBB43C57FFEB6E39AB07E" captionStart="Figure 2" captionStartId="20.[192,261,1419,1443]" captionTargetBox="[193,1440,268,1379]" captionTargetId="figure-157@20.[192,1440,266,1382]" captionTargetPageId="20" captionText="Figure 2. Phylogenetic trees of Achlyodini inferred from protein-coding regions in a) the Z chromosome and b) the mitochondrial genome (in segments). See Fig. 1 legend for other notations." pageId="19" pageNumber="18">Fig. 2</figureCitation>
) and therefore is a new species. This new species keys to
<taxonomicName id="4C28CBACFFCCBB43C353FD55E0E6B07E" baseAuthorityName="Hewitson" baseAuthorityYear="1868" box="[857,947,514,540]" class="Insecta" family="Hesperiidae" genus="Pythonides" kingdom="Animalia" order="Lepidoptera" pageId="19" pageNumber="18" phylum="Arthropoda" rank="species" species="lerina">
<emphasis id="B95C6C3DFFCCBB43C353FD55E0E6B07E" box="[857,947,514,540]" italics="true" pageId="19" pageNumber="18">P. lerina</emphasis>
</taxonomicName>
(E.41.4) in
<bibRefCitation id="EFB9CDDEFFCCBB43C432FD55E79FB07F" author="Evans WH" box="[1080,1226,514,541]" pageId="19" pageNumber="18" pagination="26 - 53" refId="ref77110" refString="Evans WH. 1953. A catalogue of the American Hesperiidae indicating the classification and nomenclature adopted in the British Museum (Natural History). Part III. Pyrginae. Section 2. The Trustees of the British Museum (Natural History); London. v + 246 p., pl. 26 - 53." type="book chapter" year="1953">Evans (1953)</bibRefCitation>
and differs from it by a more elongated lower hyaline spot in the forewing discal cell, less developed network of brown “webbing” on the ventral hindwing (
<figureCitation id="1313ACAAFFCCBB43C1E9FD10E10AB002" box="[483,607,581,608]" captionStart="Figures 3348" captionStartId="96.[192,271,1841,1865]" captionTargetBox="[198,1434,372,1745]" captionTargetId="figure-67@96.[192,1440,361,1751]" captionTargetPageId="96" captionText="Figures 3348. Holotypes, data in text. 3334) Ridens angulinea sp. n. 3536) Pythonides lera sp. n. 3738) Pythonides latemarginatus sp. n. 3940) Gindanes variegatus sp. n. 4142) Milanion (Milanion) virga sp. n. 4344) Milanion (Milanion) furvus sp. n. 4546) Milanion (Milanion) laricus sp. n. 4748) Charidia ronda sp. n." pageId="19" pageNumber="18">Fig. 3536</figureCitation>
), and more robust harpe that protrudes dorsad of costa (249). Due to the cryptic nature of this species, most reliable identification is achieved by DNA and a combination of the following base pairs is diagnostic in the nuclear genome: aly393.15.7:G381C, aly393.15.7:A395T, aly531.17.6:C195T, aly82.4.5:A198T, aly173.28.2:A84G, aly345.5.1:C36C (not T), aly
<date id="FF9696EFFFCCBB43C3A3FDFDE75FB0A7" box="[937,1034,683,709]" pageId="19" pageNumber="18" value="2116-06-01">2116.6.1</date>
:C247C (not T), aly221.16.10:A57A (not T), aly3512.5.3:T141T (not C), aly2012.21.2:C863C (not G), and COI barcode: T79C, T133T, A190T, T280C, T536C.
</paragraph>
<paragraph id="8B97B02FFFCCBB43C0CAFC4EE7D8B184" blockId="19.[192,1440,792,998]" pageId="19" pageNumber="18">
<emphasis id="B95C6C3DFFCCBB43C0CAFC4EE11BB150" bold="true" box="[192,590,792,818]" pageId="19" pageNumber="18">
Barcode sequence of the
<typeStatus id="54930E8DFFCCBB43C1EAFC4EE11CB150" box="[480,585,792,818]" pageId="19" pageNumber="18" type="holotype">holotype</typeStatus>
.
</emphasis>
Sample NVG-18018B09, GenBank OR837637, 658 base pairs: AACTTTATATTTTATTTTTGGAATTTGAGCTGGTATAGTTGGAACATCTTTAAGTTTATTAATTCGAACTGAACTAGGCAACCCTGGTTCTTTAATT GGAGATGATCAAATTTATAATACCATTGTAACTGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGTAATT GACTAGTACCCCTTATACTAGGAGCTCCTGATATAGCCTTTCCTCGAATAAATAATATAAGTTTTTGACTATTACCTCCTTCTCTCACATTATTAAT TTCAAGAAGTATTGTAGAAAATGGAACTGGAACTGGATGAACAGTTTACCCCCCTCTTTCTTCTAATATTGCTCATCAAGGTTCTTCCGTAGATTTA GCTATTTTTTCCCTGCATTTAGCTGGAATTTCATCAATTTTAGGTGCTATTAATTTTATTACTACAATTATTAATATACGAATTAGAAACCTTTCTT TTGATCAAATACCTTTATTCGTTTGAGCAGTAGGAATTACAGCATTACTTCTACTATTATCATTACCTGTTTTAGCAGGAGCTATTACTATACTATT AACTGATCGAAATTTAAATACATCATTTTTTGACCCTGCTGGAGGAGGAGACCCAATTTTATATCAACATTTATTT
</paragraph>
</subSubSection>
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<emphasis id="B95C6C3DFFCCBB43C0CAFCAFE23DB670" bold="true" box="[192,360,1016,1043]" pageId="19" pageNumber="18">Type material.</emphasis>
<materialsCitation id="3B40BA72FFCCBB43C164FCAFE2BAB6F8" collectingDate="2011-02-05" collectionCode="DC, USNM, V" collectorName="Kinyon &amp; Nick &amp; Grishin &amp; QR Code" country="Peru" elevation="1050" location="Smithsonian Institution" municipality="National Museum of Natural History" pageId="19" pageNumber="18" specimenCount="2" specimenCount-male="2" stateProvince="Cusco" typeStatus="holotype">
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<typeStatus id="54930E8DFFCCBB43C164FCAFE288B671" box="[366,477,1017,1043]" pageId="19" pageNumber="18" type="holotype">Holotype</typeStatus>
:
</emphasis>
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currently deposited in the
<collectingMunicipality id="6BF32A55FFCCBB43C323FCAEE793B670" box="[809,1222,1016,1043]" pageId="19" pageNumber="18">National Museum of Natural History</collectingMunicipality>
,
<location id="8EF7E6F4FFCCBB43C4DAFCAEE253B656" LSID="urn:lsid:plazi:treatment:03810139FFCCBB43C0CAFE19E709B775:8EF7E6F4FFCCBB43C4DAFCAEE253B656" country="Peru" municipality="National Museum of Natural History" name="Smithsonian Institution" pageId="19" pageNumber="18" stateProvince="Cusco">Smithsonian Institution</location>
, Washington,
<collectionCode id="ED3928EAFFCCBB43C1A1FB4CE280B656" box="[427,469,1050,1076]" pageId="19" pageNumber="18">DC</collectionCode>
, USA (
<collectionCode id="ED3928EAFFCCBB43C223FB4CE12BB656" box="[553,638,1050,1076]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34871" lsid="urn:lsid:biocol.org:col:34871" name="Smithsonian Institution, National Museum of Natural History" pageId="19" pageNumber="18" type="Museum">USNM</collectionCode>
), illustrated in
<figureCitation id="1313ACAAFFCCBB43C324FB4CE0F2B657" box="[814,935,1050,1077]" captionStart="Figures 3348" captionStartId="96.[192,271,1841,1865]" captionTargetBox="[198,1434,372,1745]" captionTargetId="figure-67@96.[192,1440,361,1751]" captionTargetPageId="96" captionText="Figures 3348. Holotypes, data in text. 3334) Ridens angulinea sp. n. 3536) Pythonides lera sp. n. 3738) Pythonides latemarginatus sp. n. 3940) Gindanes variegatus sp. n. 4142) Milanion (Milanion) virga sp. n. 4344) Milanion (Milanion) furvus sp. n. 4546) Milanion (Milanion) laricus sp. n. 4748) Charidia ronda sp. n." pageId="19" pageNumber="18">Fig. 3536</figureCitation>
, bears the following four rectangular labels, three white: [
<collectingCountry id="F33FF0BFFFCCBB43C151FB6AE2F1B634" box="[347,420,1084,1110]" name="Peru" pageId="19" pageNumber="18">PERU</collectingCountry>
:
<collectingRegion id="49EC7ECDFFCCBB43C1ADFB6AE2B8B634" box="[423,493,1084,1110]" country="Peru" name="Cusco" pageId="19" pageNumber="18">Cuzco</collectingRegion>
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</quantity>
| Quitacalzone | Cosnipata Road 1530 |
<date id="FF9696EFFFCCBB43C40AFB6AE70AB635" box="[1024,1119,1084,1111]" pageId="19" pageNumber="18" value="2011-02-05">
<collectingDate id="EFD26F07FFCCBB43C40AFB6AE70AB635" box="[1024,1119,1084,1111]" pageId="19" pageNumber="18" value="2011-02-05">5.ii.2011</collectingDate>
</date>
<collectorName id="26DDD5F9FFCCBB43C46FFB6AE7EDB634" box="[1125,1208,1084,1110]" pageId="19" pageNumber="18">Kinyon</collectorName>
], [DNA sample ID: | NVG-18018B09 | c/o
<collectorName id="26DDD5F9FFCCBB43C1B5FB0BE2A1B615" box="[447,500,1117,1143]" pageId="19" pageNumber="18">Nick</collectorName>
<collectionCode id="ED3928EAFFCCBB43C1F1FB08E15BB61A" box="[507,526,1118,1144]" country="Canada" lsid="urn:lsid:biocol.org:col:13946" name="Royal British Columbia Museum - Herbarium" pageId="19" pageNumber="18" type="Museum">V</collectionCode>
.
<collectorName id="26DDD5F9FFCCBB43C21DFB0BE138B615" box="[535,621,1117,1143]" pageId="19" pageNumber="18">Grishin</collectorName>
], [USNMENT | {
<collectorName id="26DDD5F9FFCCBB43C331FB08E0F2B615" box="[827,935,1117,1144]" pageId="19" pageNumber="18">QR Code</collectorName>
} | 01450938], and one red [
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<specimenCount id="9D2E7BA6FFCCBB43C577FB09E6C7B619" box="[1405,1426,1119,1147]" count="1" pageId="19" pageNumber="18" type="male"></specimenCount>
|
<taxonomicName id="4C28CBACFFCCBB43C0CAFB29E26BB6FB" authorityName="Hubner" authorityYear="1819" box="[192,318,1151,1177]" class="Insecta" family="Hesperiidae" genus="Pythonides" kingdom="Animalia" order="Lepidoptera" pageId="19" pageNumber="18" phylum="Arthropoda" rank="genus">Pythonides</taxonomicName>
|
<taxonomicName id="4C28CBACFFCCBB43C158FB29E28DB6FB" authority="Grishin" authorityName="Zhang &amp; Cong &amp; Grishin" authorityYear="2023" box="[338,472,1151,1177]" class="Insecta" family="Hesperiidae" genus="Pythonides" kingdom="Animalia" order="Lepidoptera" pageId="19" pageNumber="18" phylum="Arthropoda" rank="species" species="lera">lera Grishin</taxonomicName>
].
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</paragraph>
<paragraph id="8B97B02FFFCCBB43C0CAFBFFE74EB6A6" blockId="19.[192,1051,1193,1220]" box="[192,1051,1193,1220]" pageId="19" pageNumber="18">
<emphasis id="B95C6C3DFFCCBB43C0CAFBFFE20EB6A1" bold="true" box="[192,347,1193,1219]" pageId="19" pageNumber="18">
<typeStatus id="54930E8DFFCCBB43C0CAFBFFE3ACB6A1" box="[192,249,1193,1219]" pageId="19" pageNumber="18">Type</typeStatus>
locality.
</emphasis>
<collectingCountry id="F33FF0BFFFCCBB43C16BFBFCE2CDB6A6" box="[353,408,1194,1220]" name="Peru" pageId="19" pageNumber="18">Peru</collectingCountry>
:
<collectingRegion id="49EC7ECDFFCCBB43C1A8FBFFE2BFB6A1" box="[418,490,1193,1219]" country="Peru" name="Cusco" pageId="19" pageNumber="18">Cuzco</collectingRegion>
, Cosñipata Road, Quitacalzone, elevation
<quantity id="4CD01DCAFFCCBB43C3CBFBFFE742B6A6" box="[961,1047,1193,1220]" metricMagnitude="3" metricUnit="m" metricValue="1.05" pageId="19" pageNumber="18" unit="m" value="1050.0">1050 m</quantity>
.
</paragraph>
</subSubSection>
<subSubSection id="C332E3A4FFCCBB43C0CAFB85E631B68F" box="[192,1380,1235,1261]" pageId="19" pageNumber="18" type="etymology">
<paragraph id="8B97B02FFFCCBB43C0CAFB85E631B68F" blockId="19.[192,1380,1235,1261]" box="[192,1380,1235,1261]" pageId="19" pageNumber="18">
<emphasis id="B95C6C3DFFCCBB43C0CAFB85E211B68F" bold="true" box="[192,324,1235,1261]" pageId="19" pageNumber="18">Etymology.</emphasis>
The name is formed from its sister species name
<emphasis id="B95C6C3DFFCCBB43C362FB85E0D1B68F" box="[872,900,1235,1261]" italics="true" pageId="19" pageNumber="18">ler</emphasis>
[in]
<emphasis id="B95C6C3DFFCCBB43C3A4FB82E0E9B68F" box="[942,956,1236,1261]" italics="true" pageId="19" pageNumber="18">a</emphasis>
and is a feminine noun in apposition.
</paragraph>
</subSubSection>
<subSubSection id="C332E3A4FFCCBB43C0CAFBAAE709B775" box="[192,1116,1276,1303]" pageId="19" pageNumber="18" type="distribution">
<paragraph id="8B97B02FFFCCBB43C0CAFBAAE709B775" blockId="19.[192,1116,1276,1303]" box="[192,1116,1276,1303]" pageId="19" pageNumber="18">
<emphasis id="B95C6C3DFFCCBB43C0CAFBAAE20EB774" bold="true" box="[192,347,1276,1302]" pageId="19" pageNumber="18">Distribution.</emphasis>
Currently known only from the
<typeStatus id="54930E8DFFCCBB43C2CDFBAAE07CB774" box="[711,809,1276,1302]" pageId="19" pageNumber="18" type="holotype">holotype</typeStatus>
collected in southern
<collectingCountry id="F33FF0BFFFCCBB43C42BFBABE70DB775" box="[1057,1112,1277,1303]" name="Peru" pageId="19" pageNumber="18">Peru</collectingCountry>
.
</paragraph>
</subSubSection>
</treatment>
</document>