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<document id="F4D182DF82D4CB2F8059E614E6D474D7" ID-DOI="10.3897/mycokeys.105.119536" ID-publisher-id="119536" URI-arpha="54724331-66A7-5219-95B5-7306653A0A2D" XM.bibliography_approvedBy="admin" XM.materialsCitations_approvedBy="admin" XM.taxonomicNames_approvedBy="admin" article-type="research-article" checkinTime="1717010140543" checkinUser="pensoft" docAuthor="Wang, Yuchun, Tu, Yiyi, Chen, Xueling, Jiang, Hong, Ren, Hengze, Lu, Qinhua, Wei, Chaoling &amp; Lv, Wuyun" docDate="2024" docId="72A64BE607805DAB9871522A4C052DDB" docLanguage="en" docName="MycoKeys 105: 217-251" docOrigin="MycoKeys 105" docSource="https://mycokeys.pensoft.net/article/119536/download/xml/" docStyle="DocumentStyle:PensoftTaxPub.0000.journal_article.mycokeys" docStyleName="PensoftTaxPub.0000.journal_article.mycokeys" docTitle="Neoascochyta yunnanensis Y. Wang, Y. Tu, X. Chen, H. Jiang, H. Ren, Q. Lu, C. Wei &amp; W. Lv 2024, sp. nov." docType="treatment" docVersion="3" dtd-version="3.0" lastPageNumber="251" masterDocId="5472433166A7521995B57306653A0A2D" masterDocTitle="Didymellaceae species associated with tea plant (Camellia sinensis) in China" masterLastPageNumber="251" masterPageNumber="217" pageNumber="217" updateTime="1717029935025" updateUser="admin">
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<mods:title id="50AB138461AAFA04E83DA6506C8261B2">Didymellaceae species associated with tea plant (Camellia sinensis) in China</mods:title>
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<mods:name id="617C27D9D86EA8F75E176B74FDB2086D" type="personal">
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<mods:namePart id="8DFA662DE056A6BFC1750BD234255DF3">Wang, Yuchun</mods:namePart>
<mods:affiliation id="E5CEB9EB66129192EB6297378997D643">College of Tea Science and Tea Culture, Zhejiang A &amp; F University, Hangzhou 311300, Zhejiang, China</mods:affiliation>
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<mods:namePart id="BCD126E1A03B2EB29B8448447F118D30">Tu, Yiyi</mods:namePart>
<mods:affiliation id="36D48112132E22DFE8F86FD2D9342ED4">College of Tea Science and Tea Culture, Zhejiang A &amp; F University, Hangzhou 311300, Zhejiang, China</mods:affiliation>
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<mods:namePart id="42E5A730378912812C8B55B5E19739D2">Chen, Xueling</mods:namePart>
<mods:affiliation id="196A59944CC1DBEFFC945E66DE95DE4E">College of Tea Science and Tea Culture, Zhejiang A &amp; F University, Hangzhou 311300, Zhejiang, China</mods:affiliation>
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<mods:namePart id="25CA1AC1B972B2BFBD342545ED8846FD">Jiang, Hong</mods:namePart>
<mods:affiliation id="86517CB57FE36F4FCE23E336767ACEBA">College of Tea Science and Tea Culture, Zhejiang A &amp; F University, Hangzhou 311300, Zhejiang, China</mods:affiliation>
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<mods:namePart id="4BBB5532E2F4A70AE4C3E6DEBFD92912">Ren, Hengze</mods:namePart>
<mods:affiliation id="9896DF8AFDBEEBF07EE3DF8EBAB6F3FA">College of Tea Science and Tea Culture, Zhejiang A &amp; F University, Hangzhou 311300, Zhejiang, China</mods:affiliation>
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<mods:namePart id="0C6FD1B69C78CFFD15C03C6BF2B1AB62">Lu, Qinhua</mods:namePart>
<mods:affiliation id="AD4984EC162D9BF6D347F1C1967201A5">Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China</mods:affiliation>
</mods:name>
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<mods:namePart id="05E51CB7D0F018B8596429C0885D81FF">Wei, Chaoling</mods:namePart>
<mods:affiliation id="552B40E287605CEFBF34E27C818250F4">State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, Anhui, China</mods:affiliation>
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<mods:namePart id="3F801744B0B3473A4855BA9ED67171B5">Lv, Wuyun</mods:namePart>
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<mods:affiliation id="515CADE0CC983A0E9424C1328F09C908">College of Tea Science and Tea Culture, Zhejiang A &amp; F University, Hangzhou 311300, Zhejiang, China</mods:affiliation>
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<mods:date id="90983AE9E7B042C3231F928BAB4AB9DE">2024</mods:date>
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<mods:number id="EEFC49766A1FAED5666707612C7B36E3">2024-05-29</mods:number>
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<mods:number id="5A95F5B78FEEF07C6C3A4DD4A6CF6DD6">105</mods:number>
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<mods:identifier id="AB9B6D00408FEC93661CD81103262A46" type="DOI">10.3897/mycokeys.105.119536</mods:identifier>
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<treatment id="72A64BE607805DAB9871522A4C052DDB" ID-DOI="http://doi.org/10.5281/zenodo.11391023" ID-Zenodo-Dep="11391023" ID-arpha="72A64BE6-0780-5DAB-9871-522A4C052DDB" ID-mycobank="849001" LSID="urn:lsid:plazi:treatment:72A64BE607805DAB9871522A4C052DDB" httpUri="http://treatment.plazi.org/id/72A64BE607805DAB9871522A4C052DDB">
<subSubSection id="A918CD23A9DB15D3570AB54CD4AE925A" type="nomenclature">
<paragraph id="80C24802249341E3F5FEAC48005B1D18">
<taxonomicName id="445F14BF5F1FF01DBA164F6AE8FF43DC" authority="Y. Wang, Y. Tu, X. Chen, H. Jiang, H. Ren, Q. Lu, C. Wei &amp; W. Lv" authorityName="Y. Wang, Y. Tu, X. Chen, H. Jiang, H. Ren, Q. Lu, C. Wei &amp; W. Lv" authorityYear="2024" class="Dothideomycetes" family="Didymellaceae" genus="Neoascochyta" kingdom="Fungi" order="Pleosporales" phylum="Ascomycota" rank="species" species="yunnanensis" status="sp. nov.">
<emphasis id="740F02983D95C456D43413D7FE5DD4AA" italics="true">Neoascochyta yunnanensis</emphasis>
Y. Wang, Y. Tu, X. Chen, H. Jiang, H. Ren, Q. Lu, C. Wei &amp; W. Lv
</taxonomicName>
<taxonomicNameLabel id="8CBDD9C367B4FD3710EA42675893CFDB" rank="species">sp. nov.</taxonomicNameLabel>
</paragraph>
</subSubSection>
<subSubSection id="DB69F515E387BD02FE0BEC56B09E8F97" type="description">
<paragraph id="3E2908B976E46266C6267EA8DB00AF65">
<figureCitation id="6A1E569776583EC06CBFE743ECAF13B2" captionStart="Figure 9" captionStartId="F9" captionText="Figure 9. Neoascochyta yunnanensis YCW 1883 A, B colony on PDA (front and reverse) C, D colony on OA (front and reverse) E mycelia F pycnidia forming on PDA G conidia. Scale bars: 10 μm (E G)." figureDoi="10.3897/mycokeys.105.119536.figure9" httpUri="https://binary.pensoft.net/fig/1058577">Fig. 9</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="SECID0E5PCI" type="etymology">
<paragraph id="BCCA391A5B02F9D8A76FF5EF43B6C689">
<heading id="30B08286D4707F7B89F016CF41739D30" reason="title">Etymology.</heading>
</paragraph>
<paragraph id="124CA74C4D4E41889AD94A5D406F8CCA">
Named after the location where it was collected,
<collectingRegion id="23D4AD107A161D125A76AFD74C9D83D8" country="China" name="Yunnan">Yunnan Province</collectingRegion>
.
</paragraph>
</subSubSection>
<subSubSection id="SECID0EDQCI" type="description">
<paragraph id="883524EDE5B11E14EB16FB4373BBCB53">
<heading id="FD087B63BDA8373A24D1A0CAC9E1DF2B" reason="title">Description.</heading>
</paragraph>
<paragraph id="9F300DEB7DE7159DBF8F7F19B15A3584">
<emphasis id="B2EB8F496A9C55FACFFCE3E31198B31D" bold="true" italics="true">Sexual morph</emphasis>
: undetermined.
<emphasis id="832530656418211E9EBE2C5C3E7C8705" bold="true" italics="true">Asexual morph</emphasis>
: Pycnidia smooth, subglobose to ellipsoidal, hyaline. Conidia ellipsoidal to subcylindrical, pale yellow to green, smooth- and thin-walled, abundant, generated from pycnidia, aseptate, 8.511.7 × 4.57 µm (av. = 9.9 ± 0.9 × 5.4 ± 0.6 µm, n = 30). Mycelia lateral branching, septate, hyaline.
</paragraph>
</subSubSection>
<subSubSection id="SECID0ERQCI" type="description">
<paragraph id="0A3D682AFDFCD3CAA36005AE070AB2D9">
<heading id="D542CA9D9BD3F729FD2D7C14CFC31098" reason="title">Culture characteristics.</heading>
</paragraph>
<paragraph id="BB05268BAEC9F90A0214CA76C029066C">
Colonies on PDA reaching
<quantity id="A78F0D54D2FD2CD9E2A8D2CC3888A7D5" metricMagnitude="-2" metricUnit="m" metricValue="4.35" metricValueMax="4.5" metricValueMin="4.2" unit="mm" value="43.5" valueMax="45.0" valueMin="42.0">4245 mm</quantity>
diam. after 7 days 28 ° C in the dark, margin regular, aerial mycelium flat, whitish; reverse black to pale buff. Pycnidia and conidia produced on the colony surface after being cultured for 14 days at 28 ° C in the dark. Colonies on
<abbrev id="ABBRID0EXQCI" xlink_title="oatmeal agar">OA</abbrev>
reaching
<quantity id="4268B834E225B7A1FBD3B906EA4AA497" metricMagnitude="-2" metricUnit="m" metricValue="3.65" metricValueMax="3.9" metricValueMin="3.4" unit="mm" value="36.5" valueMax="39.0" valueMin="34.0">34 39 mm</quantity>
diam. after 7 days at 28 ° C in the dark, margin irregular, aerial mycelium flat, black in the centre, white edges; reverse concolorous.
</paragraph>
<caption id="293830C394AFD091315CD424767419B9" ID-DOI="10.3897/mycokeys.105.119536.figure9" ID-arpha="DF7897FC-A4E3-5E1B-ADBA-38ADD9D00FC6" httpUri="https://binary.pensoft.net/fig/1058577" startId="F9">
<paragraph id="C303E21F8AE42327F34543F1258AED4E">
<label id="EE2983642BC8BF70829BF1F2391C3AD9">Figure 9.</label>
</paragraph>
<paragraph id="18CDF61BD21BA98BBFA09F0F1A9CB3AB">
<taxonomicName id="0802BE478FDFBABC352F000EC380D255" authority="YCW 1883" authorityName="YCW" authorityYear="1883" class="Dothideomycetes" family="Didymellaceae" genus="Neoascochyta" kingdom="Fungi" order="Pleosporales" phylum="Ascomycota" rank="species" species="yunnanensis">
<emphasis id="40B9DD729DFACD723B11B2A7788F6075" italics="true">Neoascochyta yunnanensis</emphasis>
<specimenCode id="20016C35EBB32F47E56E4DD4CD2AC3C8">YCW 1883</specimenCode>
</taxonomicName>
<emphasis id="AEB2817BFAA4C5AEC5C1A46B6726F7B1" bold="true">A, B</emphasis>
colony on PDA (front and reverse)
<emphasis id="347EBCC70F30BE80B3D63886EA3D6F51" bold="true">C, D</emphasis>
colony on
<abbrev id="ABBRID0ESRCI" xlink_title="oatmeal agar">OA</abbrev>
(front and reverse)
<emphasis id="724291AF6B751CE8A6ED17D7B0EA5F6D" bold="true">E</emphasis>
mycelia
<emphasis id="BA11FC494D48D9450D54704E674A9042" bold="true">F</emphasis>
pycnidia forming on PDA
<emphasis id="810A11939B9B1A1F079614893A3AE961" bold="true">G</emphasis>
conidia. Scale bars: 10 μm (
<emphasis id="B7EEC10385852277DAD64108354CECC0" bold="true">E G</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection id="SECID0EDSCI" type="material">
<paragraph id="BCB652B89D1B698F41D04BF5BA9DE940">
<heading id="D976FEB34C6230D11BED26E6D2221B4E" reason="title">Materials examined.</heading>
</paragraph>
<paragraph id="CE4E7BF15B12E17EE4E4D75008C8AFA0">
<materialsCitation id="A51E56B39518C7267357A10B37ADBF6E" collectedFrom="from healthy leaves of C. sinensis" collectingDate="2020-06-13" collectionCode="Y, C" collectorName="Y. C. Wang" country="China" county="Jingdong Yizu Autonomous County" location="Puer City" specimenCode="HMAS 352391, CGMCC 3.24253, YCW 1883" specimenCount="1" stateProvince="Yunnan Province" typeStatus="holotype">
<collectingCountry id="D6F12E71612C2E9001BF75239CBF21B9" name="China">China</collectingCountry>
,
<collectingRegion id="AF06554D50D8D3D04E14759E098DEA57" country="China" name="Yunnan">Yunnan Province</collectingRegion>
,
<location id="D3F9C147A42C97D5DBB6D1AB062F982B" LSID="urn:lsid:plazi:treatment:72A64BE607805DAB9871522A4C052DDB:D3F9C147A42C97D5DBB6D1AB062F982B" country="China" county="Jingdong Yizu Autonomous County" name="Puer City" stateProvince="Yunnan Province">Puer City</location>
,
<collectingCounty id="0AD47C038EFB3C9BB35B0E27CF34F5AA">Jingdong Yizu Autonomous County</collectingCounty>
,
<collectedFrom id="BB1C0E9EB717FA9C0C0391DB6814D57A">
from healthy leaves of
<taxonomicName id="6313B39D71869DD793C3AF6CA33F9BCF" authorityName="YCW" authorityYear="1884" class="Magnoliopsida" family="Theaceae" genus="Camellia" kingdom="Plantae" order="Ericales" phylum="Tracheophyta" rank="species" species="sinensis">
<emphasis id="3C691D76E7C66E3260DA7A03335D1706" italics="true">C. sinensis</emphasis>
</taxonomicName>
</collectedFrom>
,
<date id="4187BF32341035B7F8C41AB37E71D3E3" value="2020-06-13">
<collectingDate id="F884B8E5990384E89A86201DFCAE95CF" value="2020-06-13">13 Jun 2020</collectingDate>
</date>
,
<collectorName id="76751BA2E3F5E031590B6B20E3069B8F">Y. C. Wang</collectorName>
,
<typeStatus id="E300A0C2850F6F678D5D4789FDE70092" type="holotype">Holotype</typeStatus>
<specimenCode id="724FA95C851D43E4AEED24251DC4521B" collectionCode="HMAS" country="China" httpUri="http://grbio.org/cool/qht3-g0qs" name="Chinese Academy of Sciences" type="Herbarium">
<named-content id="7BB069616A6A8A7C3DF16B5489712510" content-type="dwc:institutional_code" xlink_href="http://grbio.org/institution/chinese-academy-sciences" xlink_title="Mycological Herbarium, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China">HMAS</named-content>
352391
</specimenCode>
, culture ex-type
<specimenCode id="E8ED1130877557045D1A6026FBB8FCB8" collectionCode="CGMCC" country="China" httpUri="http://grbio.org/cool/myms-whqv" name="China General Microbiological Culture Collection Center, Chinese Academy of Sciences" type="">
<named-content id="94B158A4A4EDF02093919FFB5875C7B1" content-type="dwc:institutional_code" xlink_href="http://grbio.org/institution/china-general-microbiological-culture-collection-center-chinese-academy-sciences" xlink_title="China General Microbiological Culture Collection Center">CGMCC</named-content>
3.24253
</specimenCode>
=
<specimenCode id="216D11CAFEDCA235E2B1BC8D363C3911">YCW 1883</specimenCode>
.
</materialsCitation>
</paragraph>
</subSubSection>
<subSubSection id="SECID0E5SCI" type="notes">
<paragraph id="657A4CC8B4644CCFF37FABC4719B997C">
<heading id="B7F2B7183ACEAA4F348D24E2EB0CACE8" reason="title">Notes.</heading>
</paragraph>
<paragraph id="E804309A479AB4FF3541151FDA6C7CCA">
<taxonomicName id="B7884D72ABB8891B9C940A17FB3A9401" authorityName="YCW" authorityYear="1883" class="Dothideomycetes" family="Didymellaceae" genus="Neoascochyta" kingdom="Fungi" order="Pleosporales" phylum="Ascomycota" rank="species" species="yunnanensis">
<emphasis id="A9ED9F0440676627CCA734C9538A8747" italics="true">Neoascochyta yunnanensis</emphasis>
</taxonomicName>
is closely related to
<taxonomicName id="61877B2D5809BFCC6292BC417061DC26" class="Dothideomycetes" family="Didymellaceae" genus="Neoascochyta" kingdom="Fungi" order="Pleosporales" phylum="Ascomycota" rank="species" species="rosicola">
<emphasis id="7E3646C1067ED945760A575735311AE6" italics="true">N. rosicola</emphasis>
</taxonomicName>
with high statistical support (99 % / 1,
<abbrev id="ABBRID0E1TCI" xlink_title="Maximum Likelihood">ML</abbrev>
/ PP, Fig.
<figureCitation id="A45D2D2DF3AB76C8139796DD867961A3" captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Phylogenetic tree generated by Maximum Likelihood analysis, based on the combined ITS, LSU, RPB 2 and TUB 2 dataset of Neoascochyta, Paraboeremia, Remotididymella and Stagonosporopsis species. Bootstrap support values above 50 % and Bayesian posterior values above 0.75 are shown at each node (ML / PP). Pleiochaeta setosa CBS 118.25 / CBS 496.63 is used as the outgroup. Ex-type strains are emphasised in bold." figureDoi="10.3897/mycokeys.105.119536.figure4" httpUri="https://binary.pensoft.net/fig/1058572">4</figureCitation>
).
<taxonomicName id="28D9ACEBECE41206209095D38FF04081" authorityName="YCW" authorityYear="1883" class="Dothideomycetes" family="Didymellaceae" genus="Neoascochyta" kingdom="Fungi" order="Pleosporales" phylum="Ascomycota" rank="species" species="yunnanensis">
<emphasis id="98959D7145C065ABF3241899FBEAEE98" italics="true">Neoascochyta yunnanensis</emphasis>
</taxonomicName>
has 2 bp differences in ITS from
<taxonomicName id="83A7DA7D7939BE463B67E20C57D68F2E" class="Dothideomycetes" family="Didymellaceae" genus="Neoascochyta" kingdom="Fungi" order="Pleosporales" phylum="Ascomycota" rank="species" species="rosicola">
<emphasis id="36D92C364CEE87E3D787A0970AC7F508" italics="true">N. rosicola</emphasis>
</taxonomicName>
. In the present study, one strain was isolated from healthy tea plant leaves. It was isolated and identified from tea plant for the first time.
</paragraph>
</subSubSection>
</treatment>
</document>