Didymellaceae species associated with tea plant (Camellia sinensis) in China Author Wang, Yuchun College of Tea Science and Tea Culture, Zhejiang A & F University, Hangzhou 311300, Zhejiang, China Author Tu, Yiyi College of Tea Science and Tea Culture, Zhejiang A & F University, Hangzhou 311300, Zhejiang, China Author Chen, Xueling College of Tea Science and Tea Culture, Zhejiang A & F University, Hangzhou 311300, Zhejiang, China Author Jiang, Hong College of Tea Science and Tea Culture, Zhejiang A & F University, Hangzhou 311300, Zhejiang, China Author Ren, Hengze College of Tea Science and Tea Culture, Zhejiang A & F University, Hangzhou 311300, Zhejiang, China Author Lu, Qinhua Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China Author Wei, Chaoling State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, Anhui, China Author Lv, Wuyun 0000-0003-3781-0763 College of Tea Science and Tea Culture, Zhejiang A & F University, Hangzhou 311300, Zhejiang, China text MycoKeys 2024 2024-05-29 105 217 251 journal article 10.3897/mycokeys.105.119536 Neoascochyta yunnanensis Y. Wang, Y. Tu, X. Chen, H. Jiang, H. Ren, Q. Lu, C. Wei & W. Lv sp. nov. Fig. 9 Etymology. Named after the location where it was collected, Yunnan Province . Description. Sexual morph : undetermined. Asexual morph : Pycnidia smooth, subglobose to ellipsoidal, hyaline. Conidia ellipsoidal to subcylindrical, pale yellow to green, smooth- and thin-walled, abundant, generated from pycnidia, aseptate, 8.5–11.7 × 4.5–7 µm (av. = 9.9 ± 0.9 × 5.4 ± 0.6 µm, n = 30). Mycelia lateral branching, septate, hyaline. Culture characteristics. Colonies on PDA reaching 42–45 mm diam. after 7 days 28 ° C in the dark, margin regular, aerial mycelium flat, whitish; reverse black to pale buff. Pycnidia and conidia produced on the colony surface after being cultured for 14 days at 28 ° C in the dark. Colonies on OA reaching 34 – 39 mm diam. after 7 days at 28 ° C in the dark, margin irregular, aerial mycelium flat, black in the centre, white edges; reverse concolorous. Neoascochyta yunnanensis YCW 1883 A, B colony on PDA (front and reverse) C, D colony on OA (front and reverse) E mycelia F pycnidia forming on PDA G conidia. Scale bars: 10 μm ( E – G ). Materials examined. China , Yunnan Province , Puer City , Jingdong Yizu Autonomous County , from healthy leaves of C. sinensis , 13 Jun 2020 , Y. C. Wang , Holotype HMAS 352391 , culture ex-type CGMCC 3.24253 = YCW 1883 . Notes. Neoascochyta yunnanensis is closely related to N. rosicola with high statistical support (99 % / 1, ML / PP, Fig. 4 ). Neoascochyta yunnanensis has 2 bp differences in ITS from N. rosicola . In the present study, one strain was isolated from healthy tea plant leaves. It was isolated and identified from tea plant for the first time.