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<document id="3AB235B92514D3F8E517B9F1B44404BB" ID-DOI="10.12651/JSR.2020.9.2.085" ID-ISSN="2713-8615" ID-Zenodo-Dep="13140525" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1722403497596" checkinUser="felipe" docAuthor="Han, Joo Hyun, Maeng, Soohyun, Park, Yuna, Lee, Sang Eun, Lee, Byoung-Hee &amp; Kim, Ki-eun Lee and Myung Kyum" docDate="2020" docId="5E1987B8770D55032858A35124BAFA02" docLanguage="en" docName="JSpeciesRes.9.2.85-104.pdf" docOrigin="Journal of Species Research 9 (2)" docStyle="DocumentStyle:4D398BFF45FF8CA9E08F546BB06AE372.2:JSpeciesRes.2012-.journal_article" docStyleId="4D398BFF45FF8CA9E08F546BB06AE372" docStyleName="JSpeciesRes.2012-.journal_article" docStyleVersion="2" docTitle="Noviherbaspirillum canariense BT" docType="treatment" docVersion="2" lastPageNumber="97" masterDocId="A220FFC07707550F2B1FA51B2773FFF7" masterDocTitle="Identification of 12 radiation-resistant bacterial species in the phylum Proteobacteria new to Korea" masterLastPageNumber="104" masterPageNumber="85" pageNumber="95" updateTime="1722403955945" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:title id="0FE523A8452B95F117493355A2E36814">Identification of 12 radiation-resistant bacterial species in the phylum Proteobacteria new to Korea</mods:title>
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<mods:namePart id="73142FBB3EEC98E42E36E8660B9E4A62">Han, Joo Hyun</mods:namePart>
<mods:affiliation id="99CCA6B55111BB432C5D65732DE80CD7">Department of Bio &amp; Environmental Technology, College of Natural Science, Seoul Womens University, Seoul 01797, Republic of Korea</mods:affiliation>
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<mods:namePart id="E2F16CE5597C58A20AB0708EB3183318">Maeng, Soohyun</mods:namePart>
<mods:affiliation id="17AB3118E09C780C7B0012C76D5A3A20">Department of Bio &amp; Environmental Technology, College of Natural Science, Seoul Womens University, Seoul 01797, Republic of Korea</mods:affiliation>
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<mods:namePart id="4DC2C2277D4325BBCDD796070B6A5BB3">Park, Yuna</mods:namePart>
<mods:affiliation id="0288F0F2811984061990938C3A542E12">Department of Bio &amp; Environmental Technology, College of Natural Science, Seoul Womens University, Seoul 01797, Republic of Korea</mods:affiliation>
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<mods:namePart id="47681867281338857FE4E00ACC8CBEF3">Lee, Sang Eun</mods:namePart>
<mods:affiliation id="0DC0124C46523DDFEBE91D09C8B1F071">Department of Bio &amp; Environmental Technology, College of Natural Science, Seoul Womens University, Seoul 01797, Republic of Korea</mods:affiliation>
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<mods:namePart id="24E92EBC6C5FCDB0A7BA593D446E11C0">Lee, Byoung-Hee</mods:namePart>
<mods:affiliation id="20A5F5A4D993659C93C2EBF793719319">Environmental Research Complex, National Institute of Biological Resources, Incheon 22689, Republic of Korea</mods:affiliation>
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<mods:namePart id="6364F5CC2CA8F2DD20B0F77478024C0F">Kim, Ki-eun Lee and Myung Kyum</mods:namePart>
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<mods:date id="9D7698F9E0A8EAB08965101EF19D1564">2020</mods:date>
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<mods:number id="50F75ABD0DC47B80DD73AF9D4EFCEF29">9</mods:number>
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<treatment id="5E1987B8770D55032858A35124BAFA02" LSID="urn:lsid:plazi:treatment:5E1987B8770D55032858A35124BAFA02" httpUri="http://treatment.plazi.org/id/5E1987B8770D55032858A35124BAFA02" lastPageId="12" lastPageNumber="97" pageId="10" pageNumber="95">
<subSubSection id="9EAA6525770D55032858A351254DF88F" lastPageId="12" lastPageNumber="97" pageId="10" pageNumber="95" type="nomenclature">
<paragraph id="D60F36AE770D55052858A35122E5F995" blockId="10.[839,1430,1610,1634]" box="[839,1430,1610,1634]" pageId="10" pageNumber="95">
<heading id="8D4781C2770D55052858A35122E5F995" box="[839,1430,1610,1634]" centered="true" fontSize="10" level="2" pageId="10" pageNumber="95" reason="5">
<emphasis id="E4C4EABC770D55052858A35122E5F995" bold="true" box="[839,1430,1610,1634]" pageId="10" pageNumber="95">
Description of
<taxonomicName id="11B04D2D770D550528F1A351221DF995" authority="BT" authorityName="BT" box="[1006,1390,1610,1634]" class="Gammaproteobacteria" family="Burkholderiaceae" genus="Noviherbaspirillum" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Burkholderiales" pageId="10" pageNumber="95" phylum="Proteobacteria" rank="species" species="canariense">
<emphasis id="E4C4EABC770D550528F1A3512237F995" bold="true" box="[1006,1348,1610,1634]" italics="true" pageId="10" pageNumber="95">Noviherbaspirillum canariense</emphasis>
BT
</taxonomicName>
339
</emphasis>
</heading>
</paragraph>
<paragraph id="D60F36AE770D55032841A360254DF88F" blockId="10.[839,1444,1659,1973]" lastBlockId="12.[174,779,1501,1977]" lastPageId="12" lastPageNumber="97" pageId="10" pageNumber="95">
Cells are Gram-stain-negative, non-flagellated, and rod-shaped. Colonies are Yellow-colored after 3 days of incubation on R2A at 25°C. In the BIOLOG GEN III, positive for pH 6,
<subScript id="4A3434EB770D55052F10A3F92353F903" attach="right" box="[1039,1056,1756,1780]" fontSize="7" pageId="10" pageNumber="95">L-</subScript>
aspartic acid,
<subScript id="4A3434EB770D55052FA9A3F923B4F903" attach="right" box="[1206,1223,1756,1780]" fontSize="7" pageId="10" pageNumber="95">L-</subScript>
glutamic acid, lincomycin, quinic acid, tetrazolium blue, L- lactic acid, D- malic acid, L- malic acid, acetic acid and aztreonam; weak positive for 1% sodium lactate, rifamycin SV, D- gluconic acid, glucuronamide, D- saccharic acid, tetrazolium violet,
<emphasis id="E4C4EABC770D5505286DA265240CF862" box="[882,895,1918,1941]" italics="true" pageId="10" pageNumber="95">p</emphasis>
-hydroxy-pheylacetic acid, methyl pyruvate, citric acid,
<emphasis id="E4C4EABC770D5505289DA28524E1F842" box="[898,914,1950,1973]" italics="true" pageId="10" pageNumber="95">α</emphasis>
-keto-glutaric acid, nalidixic acid,
<emphasis id="E4C4EABC770D55052E18A2852264F842" box="[1287,1303,1950,1973]" italics="true" pageId="10" pageNumber="95">α</emphasis>
-hydroxy-butyric acid,
<emphasis id="E4C4EABC770C55042A16A338266BF9CC" box="[265,280,1571,1595]" italics="true" pageId="11" pageNumber="96">β</emphasis>
-hydroxy-D, L- butyric acid,
<emphasis id="E4C4EABC770C55042952A33F252EF9CC" box="[589,605,1572,1595]" italics="true" pageId="11" pageNumber="96">α</emphasis>
-keto butyric acid, acetoacetic acid and propionic acid; but negative for dextrin, D- maltose, D- trehalose, D- cellobiolose, gentiobiose, sucrose,
<subScript id="4A3434EB770C55042A5EA3912627F96C" attach="right" box="[321,340,1668,1691]" fontSize="7" pageId="11" pageNumber="96">D-</subScript>
turanose, stachyose, pH 5,
<subScript id="4A3434EB770C55042969A39125FAF96B" attach="right" box="[630,649,1668,1692]" fontSize="7" pageId="11" pageNumber="96">D-</subScript>
raffinose,
<emphasis id="E4C4EABC770C55042B8FA3BE27D3F94B" box="[144,160,1701,1724]" italics="true" pageId="11" pageNumber="96">α</emphasis>
-D- lactose, D- melibiose,
<emphasis id="E4C4EABC770C55042A82A3BF26DFF94B" box="[413,428,1700,1724]" italics="true" pageId="11" pageNumber="96">β</emphasis>
-methyl-D- glucoside, D- salicin,
<emphasis id="E4C4EABC770C55042B8FA3DE27D1F92B" box="[144,162,1733,1756]" italics="true" pageId="11" pageNumber="96">N</emphasis>
-acetyl-D- glucosamine,
<emphasis id="E4C4EABC770C55042A85A3DE26DFF92B" box="[410,428,1733,1756]" italics="true" pageId="11" pageNumber="96">N</emphasis>
-acetyl-
<emphasis id="E4C4EABC770C55042AE4A3DF2579F92B" box="[507,522,1732,1756]" italics="true" pageId="11" pageNumber="96">β</emphasis>
-mannosamin,
<emphasis id="E4C4EABC770C550429B8A3DE25CAF92B" box="[679,697,1733,1756]" italics="true" pageId="11" pageNumber="96">N</emphasis>
-acetyl-D- galactosamin,
<emphasis id="E4C4EABC770C55042A7AA3FE2604F90B" box="[357,375,1765,1788]" italics="true" pageId="11" pageNumber="96">N</emphasis>
-acetyl neuraminic acid, 1% NaCl, 4% NaCl, 8% NaCl,
<emphasis id="E4C4EABC770C55042A9FA21D26E3F8EA" box="[384,400,1798,1821]" italics="true" pageId="11" pageNumber="96">α</emphasis>
-
<subScript id="4A3434EB770C55042A85A21026DCF8EA" attach="none" box="[410,431,1797,1821]" fontSize="7" pageId="11" pageNumber="96">D-</subScript>
glucose,
<subScript id="4A3434EB770C55042909A2102558F8EB" attach="right" box="[534,555,1797,1820]" fontSize="7" pageId="11" pageNumber="96">D-</subScript>
mannose,
<subScript id="4A3434EB770C550429BFA21025C7F8EA" attach="right" box="[672,692,1797,1821]" fontSize="7" pageId="11" pageNumber="96">D-</subScript>
fructose, D- galactose, 3-methyl-glucose, D- fucose, L- fucose, L- rhamnose, inosine, fusidic acid, D- serine, D- sorbitol, D- mannitol, D- arabitol, myo-inositol, glycerol, D- glucose- 6-PO
<subScript id="4A3434EB770C55042BD8A29527A3F868" attach="left" box="[199,208,1934,1951]" fontSize="7" pageId="11" pageNumber="96">4</subScript>
,
<subScript id="4A3434EB770C55042BC0A2972787F869" attach="right" box="[223,244,1926,1950]" fontSize="7" pageId="11" pageNumber="96">D-</subScript>
fructose-6-PO
<subScript id="4A3434EB770C55042A94A29526E7F868" attach="left" box="[395,404,1934,1951]" fontSize="7" pageId="11" pageNumber="96">4</subScript>
,
<subScript id="4A3434EB770C55042ABCA29726C4F869" attach="right" box="[419,439,1926,1950]" fontSize="7" pageId="11" pageNumber="96">D-</subScript>
aspartic acid,
<subScript id="4A3434EB770C5504294FA2972510F869" attach="right" box="[592,611,1926,1950]" fontSize="7" pageId="11" pageNumber="96">D-</subScript>
serine, troleandomycin, minocycline, gelatin, glyctl-L- proline, L- alanine, L- arginine, L- histidine, L- pyroglutamic acid, L- serine, guanidine HCl, niaproof 4, pectin, D- galacturonic acid, L- galactonic acid lactone, D- glucuronic acid, mucic acid, vancomycin,
<subScript id="4A3434EB770C550428ECA3912375F96B" attach="right" box="[1011,1030,1668,1692]" fontSize="7" pageId="11" pageNumber="96">D-</subScript>
lactic acid methyl ester, bromo-succinic acid, lithium chloride, potassium tellurite, tween 40,
<emphasis id="E4C4EABC770C55042836A3DE244BF92B" box="[809,824,1733,1756]" italics="true" pageId="11" pageNumber="96">γ</emphasis>
-amino-butryric acid, formic acid, sodium butyrate and sodium bromate. In API 20NE test, positive for potassium gluconate and malic acid; weak positive for indole production on tryptophan, esculin hydrolysis; but negative for reduction of nitrates (NO
<subScript id="4A3434EB770C55042F4AA256232EF8A9" attach="left" box="[1109,1117,1869,1886]" fontSize="7" pageId="11" pageNumber="96">3</subScript>
) to nitrite (NO
<subScript id="4A3434EB770C55042FE5A2562270F8A9" attach="left" box="[1274,1283,1869,1886]" fontSize="7" pageId="11" pageNumber="96">2</subScript>
<superScript id="21C59BE6770C55042E1CA258227EF8A7" attach="right" box="[1283,1293,1859,1872]" fontSize="7" pageId="11" pageNumber="96">-</superScript>
), reduction of nitrates (NO
<subScript id="4A3434EB770C550428D4A27624A7F889" attach="left" box="[971,980,1901,1918]" fontSize="7" pageId="11" pageNumber="96">3</subScript>
) to nitrogen (N
<subScript id="4A3434EB770C55042F62A27623F5F889" attach="right" box="[1149,1158,1901,1918]" fontSize="7" pageId="11" pageNumber="96">2</subScript>
), glucose fermentation, arginine dihydrolase, urease, gelatin hydrolysis (weak) and adipic acid (weak) were utilized.
<emphasis id="E4C4EABC770C55042FD0A2BD23ADF849" box="[1231,1246,1958,1982]" italics="true" pageId="11" pageNumber="96">β</emphasis>
-Galactosidase, D- glucose, L- arabinose, D- mannose, D- mannitol,
<emphasis id="E4C4EABC770B550329DDA0C525A7FA02" box="[706,724,1502,1525]" italics="true" pageId="12" pageNumber="97">N</emphasis>
-acetyl-D- glucosamine, D- maltose, capric acid, trisodium citrate and phenylacetic acid were not utilized. In API 32GN test, itaconic acid, suberic acid, sodium acetate, lactic acid, propionic acid, valeric acid, 3-hydroxybutyric acid, 3-hydroxybenzoic acid, trisodium citrate and 4-hydroxybenzoic acid were utilized whereas, L- rhamnose,
<emphasis id="E4C4EABC770B55032BB1A3DB27B3F920" box="[174,192,1728,1751]" italics="true" pageId="12" pageNumber="97">N</emphasis>
-acetyl-glucosamine, D- ribose, inositol, D- saccharose (sucrose), D- maltose, sodium malonate, L- alanine, potassium 5-ketogluconate, glycogen, L- serine, D- mannitol, D- glucose, salicin, D- melibiose, D- fucose, D- sorbitol, L- arabinose, capric acid, L- histidine, potassium 2-ketogluconate and L- proline were not utilized.
</paragraph>
</subSubSection>
<caption id="82CF6626770C55042B8FA08A245CFA2A" ID-DOI="http://doi.org/10.5281/zenodo.13140553" ID-Zenodo-Dep="13140553" httpUri="https://zenodo.org/record/13140553/files/figure.png" pageId="11" pageNumber="96" startId="11.[144,177,1425,1447]" targetBox="[408,1144,266,1398]" targetPageId="11" targetType="figure">
<paragraph id="D60F36AE770C55042B8FA08A245CFA2A" blockId="11.[144,1414,1425,1501]" pageId="11" pageNumber="96">
<emphasis id="E4C4EABC770C55042B8FA08A27A4FA51" bold="true" box="[144,215,1425,1447]" pageId="11" pageNumber="96">Fig. 10.</emphasis>
Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences shows the relationship between the strains isolated in this study and their relatives of the species
<taxonomicName id="11B04D2D770C55042AEAA0B725E5FA36" box="[501,662,1452,1473]" class="Alphaproteobacteria" family="Rhizobiaceae" genus="Rhizobium" kingdom="Bacteria" order="Rhizobiales" pageId="11" pageNumber="96" phylum="Proteobacteria" rank="species" species="alamii">
<emphasis id="E4C4EABC770C55042AEAA0B725E5FA36" box="[501,662,1452,1473]" italics="true" pageId="11" pageNumber="96">Rhizobium alamii</emphasis>
</taxonomicName>
. Bootstrap values (&gt;70%) are shown above nodes for the neighbor-joining methods. Bar: 0.01 and 0.02 substitutions per nucleotide position, respectively.
</paragraph>
</caption>
<caption id="82CF6626770B55032BB1A0502409FA60" ID-DOI="http://doi.org/10.5281/zenodo.13140558" ID-Zenodo-Dep="13140558" httpUri="https://zenodo.org/record/13140558/files/figure.png" pageId="12" pageNumber="97" startId="12.[174,207,1355,1377]" targetBox="[375,1248,260,1327]" targetPageId="12" targetType="figure">
<paragraph id="D60F36AE770B55032BB1A0502409FA60" blockId="12.[174,1444,1355,1431]" pageId="12" pageNumber="97">
<emphasis id="E4C4EABC770B55032BB1A0502787FA96" bold="true" box="[174,244,1355,1377]" pageId="12" pageNumber="97">Fig. 11.</emphasis>
Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences shows the relationship between the strains isolated in this study and their relatives of the species
<taxonomicName id="11B04D2D770B5503290AA07D259FFA8B" box="[533,748,1382,1404]" class="Gammaproteobacteria" family="Rhodocyclaceae" genus="Oryzomicrobium" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Burkholderiales" pageId="12" pageNumber="97" phylum="Proteobacteria" rank="species" species="terrae">
<emphasis id="E4C4EABC770B5503290AA07D259FFA8B" box="[533,748,1382,1404]" italics="true" pageId="12" pageNumber="97">Oryzomicrobium terrae</emphasis>
</taxonomicName>
. Bootstrap values (&gt;70%) are shown above nodes for the neighbor-joining methods. Bar: 0.01 and 0.02 substitutions per nucleotide position, respectively.
</paragraph>
</caption>
<subSubSection id="9EAA6525770B55032BDAA29A24BAFA02" pageId="12" pageNumber="97" type="description">
<paragraph id="D60F36AE770B55032BDAA29A24BAFA02" blockId="12.[174,779,1501,1977]" lastBlockId="12.[839,969,1501,1525]" pageId="12" pageNumber="97">
Strain BT339 (
<emphasis id="E4C4EABC770B55032A74A29B260CF86F" bold="true" box="[363,383,1920,1944]" pageId="12" pageNumber="97">=</emphasis>
NIBRBAC000503036) was isolated from a soil sample, Namyangju City in
<collectingRegion id="1474F84C770B55032941A2BA2405FA02" box="[606,886,1501,1977]" country="South Korea" name="Gyeonggi" pageId="12" pageNumber="97">Gyeonggi Province</collectingRegion>
,
<collectingCountry id="AEA7763E770B5503289EA0C524B5FA02" box="[897,966,1502,1525]" name="South Korea" pageId="12" pageNumber="97">Korea</collectingCountry>
.
</paragraph>
</subSubSection>
</treatment>
</document>