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<document id="7C936EE0FD89259803E02326798C30E0" ID-DOI="10.12651/JSR.2020.9.2.085" ID-ISSN="2713-8615" ID-Zenodo-Dep="13140525" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1722403497596" checkinUser="felipe" docAuthor="Han, Joo Hyun, Maeng, Soohyun, Park, Yuna, Lee, Sang Eun, Lee, Byoung-Hee &amp; Kim, Ki-eun Lee and Myung Kyum" docDate="2020" docId="5E1987B8770B55012858A3052785F97C" docLanguage="en" docName="JSpeciesRes.9.2.85-104.pdf" docOrigin="Journal of Species Research 9 (2)" docStyle="DocumentStyle:4D398BFF45FF8CA9E08F546BB06AE372.2:JSpeciesRes.2012-.journal_article" docStyleId="4D398BFF45FF8CA9E08F546BB06AE372" docStyleName="JSpeciesRes.2012-.journal_article" docStyleVersion="2" docTitle="Massilia agilis BT" docType="treatment" docVersion="2" lastPageNumber="99" masterDocId="A220FFC07707550F2B1FA51B2773FFF7" masterDocTitle="Identification of 12 radiation-resistant bacterial species in the phylum Proteobacteria new to Korea" masterLastPageNumber="104" masterPageNumber="85" pageNumber="97" updateTime="1722403955945" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:title id="48DFA5F1D547327F0C34913F631A08D2">Identification of 12 radiation-resistant bacterial species in the phylum Proteobacteria new to Korea</mods:title>
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<mods:namePart id="A45BE7454AD1C1BF8BD440A2969FDEB4">Han, Joo Hyun</mods:namePart>
<mods:affiliation id="20A68BAC092DF89D179C7001236B1840">Department of Bio &amp; Environmental Technology, College of Natural Science, Seoul Womens University, Seoul 01797, Republic of Korea</mods:affiliation>
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<mods:namePart id="2986CD8F22C097CF196B32AEABB27B51">Maeng, Soohyun</mods:namePart>
<mods:affiliation id="C6A0FEB9B495A49B89BEEADC8F56F2EA">Department of Bio &amp; Environmental Technology, College of Natural Science, Seoul Womens University, Seoul 01797, Republic of Korea</mods:affiliation>
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<mods:namePart id="B5C0FB43739FEC30B6AA6E4DE8AE528D">Park, Yuna</mods:namePart>
<mods:affiliation id="AF29717F95F617115EEDD08BD7A2F4B9">Department of Bio &amp; Environmental Technology, College of Natural Science, Seoul Womens University, Seoul 01797, Republic of Korea</mods:affiliation>
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<mods:namePart id="D85FB1B8E76FF405E63FDBF109B79ABD">Lee, Sang Eun</mods:namePart>
<mods:affiliation id="27FCC3A645524B540FF7C339239F54C8">Department of Bio &amp; Environmental Technology, College of Natural Science, Seoul Womens University, Seoul 01797, Republic of Korea</mods:affiliation>
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<mods:namePart id="8016927392B175F6D3625C1B4461B59B">Lee, Byoung-Hee</mods:namePart>
<mods:affiliation id="45008C360C2581E4BAE6F24F919E4591">Environmental Research Complex, National Institute of Biological Resources, Incheon 22689, Republic of Korea</mods:affiliation>
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<mods:date id="AB95B0B5EFA617288B37973A90B77B1D">2020</mods:date>
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<treatment id="5E1987B8770B55012858A3052785F97C" ID-DOI="http://doi.org/10.5281/zenodo.13140533" ID-Zenodo-Dep="13140533" LSID="urn:lsid:plazi:treatment:5E1987B8770B55012858A3052785F97C" httpUri="http://treatment.plazi.org/id/5E1987B8770B55012858A3052785F97C" lastPageId="14" lastPageNumber="99" pageId="12" pageNumber="97">
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<paragraph id="D60F36AE770B55032858A30523ACF9C1" blockId="12.[839,1247,1565,1590]" box="[839,1247,1565,1590]" pageId="12" pageNumber="97">
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<emphasis id="E4C4EABC770B55032858A30523ACF9C1" bold="true" box="[839,1247,1565,1590]" pageId="12" pageNumber="97">
Description of
<taxonomicName id="11B04D2D770B550328F1A30623C4F9C1" authority="BT" authorityName="BT" box="[1006,1207,1565,1590]" class="Betaproteobacteria" family="Oxalobacteraceae" genus="Massilia" kingdom="Bacteria" order="Burkholderiales" pageId="12" pageNumber="97" phylum="Proteobacteria" rank="species" species="agilis">
<emphasis id="E4C4EABC770B550328F1A30623FEF9C2" bold="true" box="[1006,1165,1565,1589]" italics="true" pageId="12" pageNumber="97">Massilia agilis</emphasis>
BT
</taxonomicName>
427
</emphasis>
</heading>
</paragraph>
<paragraph id="D60F36AE770B55012841A3452687F9DD" blockId="12.[839,1444,1630,1977]" lastBlockId="14.[174,779,1490,1675]" lastPageId="14" lastPageNumber="99" pageId="12" pageNumber="97">
Cells are Gram-stain-negative, non-flagellated, and rod-shaped. Colonies are white-colored after 3 days of incubation on R2A at 25°C. In the BIOLOG GEN III, negative for pH 6,
<subScript id="4A3434EB770B550328C6A3DE2498F920" attach="right" box="[985,1003,1727,1751]" fontSize="7" pageId="12" pageNumber="97">L-</subScript>
aspartic acid,
<subScript id="4A3434EB770B55032F64A3DE23FEF920" attach="right" box="[1147,1165,1727,1751]" fontSize="7" pageId="12" pageNumber="97">L-</subScript>
glutamic acid, lincomycin, quinic acid, tetrazolium blue, L- lactic acid, D- malic acid, L- malic acid, acetic acid, aztreonam, 1% sodium lactate, rifamycin SV, D- gluconic acid, glucuronamide, D- saccharic acid, tetrazolium violet,
<emphasis id="E4C4EABC770B55032F69A25A23F0F8AF" box="[1142,1155,1857,1880]" italics="true" pageId="12" pageNumber="97">p</emphasis>
-hydroxy-pheylacetic acid, methyl pyruvate, citric acid,
<emphasis id="E4C4EABC770B55032F6AA27A23F6F88F" box="[1141,1157,1889,1912]" italics="true" pageId="12" pageNumber="97">α</emphasis>
-keto-glutaric acid, nalidixic acid,
<emphasis id="E4C4EABC770B550328BFA29A24C3F86F" box="[928,944,1921,1944]" italics="true" pageId="12" pageNumber="97">α</emphasis>
-hydroxy-butyric acid,
<emphasis id="E4C4EABC770B55032FB3A29A23C8F86E" box="[1196,1211,1921,1945]" italics="true" pageId="12" pageNumber="97">β</emphasis>
-hydroxy-D, L- butyric acid,
<emphasis id="E4C4EABC770B5503289FA2BA24E3F84F" box="[896,912,1953,1976]" italics="true" pageId="12" pageNumber="97">α</emphasis>
-keto butyric acid, acetoacetic acid, propionic acid, dextrin, D- maltose, D- trehalose, D- cellobiolose, gentiobiose, sucrose, D- turanose, stachyose, pH 5, D- raffinose,
<emphasis id="E4C4EABC770A55022B8FA31A27D3F9EF" box="[144,160,1537,1560]" italics="true" pageId="13" pageNumber="98">α</emphasis>
-D- lactose, D- melibiose,
<emphasis id="E4C4EABC770A55022A82A31A26DFF9EE" box="[413,428,1537,1561]" italics="true" pageId="13" pageNumber="98">β</emphasis>
-methyl-D- glucoside, D- salicin,
<emphasis id="E4C4EABC770A55022B8FA33A27D1F9CF" box="[144,162,1569,1592]" italics="true" pageId="13" pageNumber="98">N</emphasis>
-
<subScript id="4A3434EB770A55022BB7A33A2676F9CE" attach="left" box="[168,261,1569,1593]" fontSize="7" pageId="13" pageNumber="98">acetyl-D-</subScript>
glucosamine,
<emphasis id="E4C4EABC770A55022A85A33A26DFF9CF" box="[410,428,1569,1592]" italics="true" pageId="13" pageNumber="98">N</emphasis>
-acetyl-
<emphasis id="E4C4EABC770A55022AE4A33A2579F9CE" box="[507,522,1569,1593]" italics="true" pageId="13" pageNumber="98">β</emphasis>
-mannosamin,
<emphasis id="E4C4EABC770A550229B8A33A25CAF9CF" box="[679,697,1569,1592]" italics="true" pageId="13" pageNumber="98">N</emphasis>
-acetyl-D- galactosamin,
<emphasis id="E4C4EABC770A55022A7AA3592604F9AE" box="[357,375,1602,1625]" italics="true" pageId="13" pageNumber="98">N</emphasis>
-acetyl neuraminic acid, 1% NaCl, 4% NaCl, 8% NaCl,
<emphasis id="E4C4EABC770A55022A9FA37926E3F98E" box="[384,400,1634,1657]" italics="true" pageId="13" pageNumber="98">α</emphasis>
-D- glucose, D- mannose, D- fructose, D- galactose, 3-methyl-glucose, D- fucose, L- fucose, L- rhamnose, inosine, fusidic acid, D- serine, D- sorbitol, D- mannitol, D- arabitol, myo-inositol, glycerol, D- glucose-6-PO
<subScript id="4A3434EB770A55022A1EA3F12679F90C" attach="left" box="[257,266,1770,1787]" fontSize="7" pageId="13" pageNumber="98">4</subScript>
, D- fructose-6-PO
<subScript id="4A3434EB770A55022AD7A3F126A2F90C" attach="right" box="[456,465,1770,1787]" fontSize="7" pageId="13" pageNumber="98">4</subScript>
, D- aspartic acid, D- serine, troleandomycin, minocycline, gelatin, glyctl-L- proline, L- alanine, L- arginine, L- histidine, L- pyroglutamic acid, L- serine, guanidine HCl, niaproof 4, pectin, D- galacturonic acid, L- galactonic acid lactone, D- glucuronic acid, mucic acid, vancomycin,
<subScript id="4A3434EB770A55022ABAA29126CBF86B" attach="right" box="[421,440,1924,1948]" fontSize="7" pageId="13" pageNumber="98">D-</subScript>
lactic acid methyl ester, bromo-succinic acid, lithium chloride, potassium tellurite, tween 40,
<emphasis id="E4C4EABC770A55022A19A2DE2666F82B" box="[262,277,1989,2012]" italics="true" pageId="13" pageNumber="98">γ</emphasis>
-amino-butryric acid, formic acid, sodium butyrate and sodium bromate. In API 20NE test, positive for urease; but negative for reduction of nitrates (NO
<subScript id="4A3434EB770A55022E47A0F12213FA0C" attach="left" box="[1368,1376,1514,1531]" fontSize="7" pageId="13" pageNumber="98">3</subScript>
) to nitrite (NO
<subScript id="4A3434EB770A55022886A31024D1F9EB" attach="left" box="[921,930,1547,1564]" fontSize="7" pageId="13" pageNumber="98">2</subScript>
<superScript id="21C59BE6770A550228BDA31A24DFF9F9" attach="right" box="[930,940,1537,1550]" fontSize="7" pageId="13" pageNumber="98">-</superScript>
), reduction of nitrates (NO
<subScript id="4A3434EB770A55022FDAA31023BEF9EB" attach="left" box="[1221,1229,1547,1564]" fontSize="7" pageId="13" pageNumber="98">3</subScript>
) to nitrogen (N
<subScript id="4A3434EB770A55022E71A3102205F9EB" attach="left" box="[1390,1398,1547,1564]" fontSize="7" pageId="13" pageNumber="98">2</subScript>
), indole production on tryptophan, glucose fermentation, arginine dihydrolase, esculin hydrolysis, gelatin hydrolysis and
<emphasis id="E4C4EABC770A55022865A37F24FAF98B" box="[890,905,1636,1660]" italics="true" pageId="13" pageNumber="98">β</emphasis>
-galactosidase. D- Maltose (weak) and malic acid (weak) were utilized whereas,
<subScript id="4A3434EB770A55022F9BA39123EAF96B" attach="right" box="[1156,1177,1668,1692]" fontSize="7" pageId="13" pageNumber="98">D-</subScript>
glucose,
<subScript id="4A3434EB770A55022E1FA3912260F96B" attach="right" box="[1280,1299,1668,1692]" fontSize="7" pageId="13" pageNumber="98">L-</subScript>
arabinose, D- mannose, D- mannitol,
<emphasis id="E4C4EABC770A55022F2CA3BE2336F94B" box="[1075,1093,1701,1724]" italics="true" pageId="13" pageNumber="98">N</emphasis>
-acetyl-D- glucosamine, potassium gluconate, capric acid, adipic acid, trisodium citrate and phenylacetic acid were not utilized. In API 32GN test, D- maltose are weakly utilized whereas, L- rhamnose,
<emphasis id="E4C4EABC770A55022836A23D2448F8CA" box="[809,827,1830,1853]" italics="true" pageId="13" pageNumber="98">N</emphasis>
-acetyl-glucosamine, D- ribose, inositol, D- saccharose (sucrose), itaconic acid, suberic acid, sodium malonate, sodium acetate, lactic acid,
<subScript id="4A3434EB770A55022F48A277231BF889" attach="right" box="[1111,1128,1894,1918]" fontSize="7" pageId="13" pageNumber="98">L-</subScript>
alanine, potassium 5-ketogluconate, glycogen, 3-hydroxybenzoic acid, L- serine, D- mannitol, D- glucose, salicin, D- melibiose, D- fucose, D- sorbitol, L- arabinose, propionic acid, capric acid, valeric acid, trisodium citrate, L- histidine, potassium 2-ketogluconate, 3-hydroxybutyric acid, 4-hydroxybenzoic acid and L- proline were not utilized.
</paragraph>
</subSubSection>
<caption id="82CF6626770A55022B90A0352428FA8D" ID-DOI="http://doi.org/10.5281/zenodo.13140560" ID-Zenodo-Dep="13140560" httpUri="https://zenodo.org/record/13140560/files/figure.png" pageId="13" pageNumber="98" startId="13.[143,176,1326,1348]" targetBox="[423,1131,257,1291]" targetPageId="13" targetType="figure">
<paragraph id="D60F36AE770A55022B90A0352428FA8D" blockId="13.[143,1413,1326,1402]" pageId="13" pageNumber="98">
<emphasis id="E4C4EABC770A55022B90A03527A5FAB4" bold="true" box="[143,214,1326,1348]" pageId="13" pageNumber="98">Fig. 12.</emphasis>
Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences shows the relationship between the strains isolated in this study and their relatives of the species
<taxonomicName id="11B04D2D770A55022AEEA05225A7FAA8" box="[497,724,1353,1375]" class="Alphaproteobacteria" family="Sphingomonadaceae" genus="Sphingomonas" kingdom="Bacteria" order="Sphingomonadales" pageId="13" pageNumber="98" phylum="Proteobacteria" rank="species" species="sanguinis">
<emphasis id="E4C4EABC770A55022AEEA05225A7FAA8" box="[497,724,1353,1375]" italics="true" pageId="13" pageNumber="98">Sphingomonas sanguinis</emphasis>
</taxonomicName>
. Bootstrap values (&gt;70%) are shown above nodes for the neighbor-joining methods. Bar: 0.01 and 0.02 substitutions per nucleotide position, respectively.
</paragraph>
</caption>
<caption id="82CF6626770955012BB1A05B24EEFA7C" ID-DOI="http://doi.org/10.5281/zenodo.13140564" ID-Zenodo-Dep="13140564" httpUri="https://zenodo.org/record/13140564/files/figure.png" pageId="14" pageNumber="99" startId="14.[174,207,1344,1366]" targetBox="[395,1221,257,1310]" targetPageId="14" targetType="figure">
<paragraph id="D60F36AE770955012BB1A05B24EEFA7C" blockId="14.[174,1444,1344,1419]" pageId="14" pageNumber="99">
<emphasis id="E4C4EABC770955012BB1A05B2786FAA2" bold="true" box="[174,245,1344,1366]" pageId="14" pageNumber="99">Fig. 13.</emphasis>
Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences shows the relationship between the strains isolated in this study and their relatives of the species
<taxonomicName id="11B04D2D77095501290AA0402471FA87" box="[533,770,1371,1392]" class="Alphaproteobacteria" family="Sphingomonadaceae" genus="Sphingomonas" kingdom="Bacteria" order="Sphingomonadales" pageId="14" pageNumber="99" phylum="Proteobacteria" rank="species" species="azotifigens">
<emphasis id="E4C4EABC77095501290AA0402471FA87" box="[533,770,1371,1392]" italics="true" pageId="14" pageNumber="99">Sphingomonas azotifigens</emphasis>
</taxonomicName>
. Bootstrap values (&gt;70%) are shown above nodes for the neighbor-joining methods. Bar: 0.01 and 0.02 substitutions per nucleotide position, respectively.
</paragraph>
</caption>
<subSubSection id="9EAA6525770955012BDAA3292785F97C" pageId="14" pageNumber="99" type="description">
<paragraph id="D60F36AE770955012BDAA3292785F97C" blockId="14.[174,779,1490,1675]" pageId="14" pageNumber="99">
Strain BT427 (
<emphasis id="E4C4EABC770955012A74A329260CF9BD" bold="true" box="[363,383,1586,1610]" pageId="14" pageNumber="99">=</emphasis>
NIBRBAC000503047) was isolated from a soil sample, Ganghwa Island in
<collectingRegion id="1474F84C77095501296CA34825BFF99C" box="[627,716,1619,1643]" country="South Korea" name="Incheon" pageId="14" pageNumber="99">Incheon</collectingRegion>
City,
<collectingCountry id="AEA7763E770955012BB1A36F2780F97C" box="[174,243,1652,1675]" name="South Korea" pageId="14" pageNumber="99">Korea</collectingCountry>
.
</paragraph>
</subSubSection>
</treatment>
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