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<document id="2CAF58CFE748EDD2486302745A5545DE" ID-DOI="10.12651/JSR.2020.9.2.085" ID-ISSN="2713-8615" ID-Zenodo-Dep="13140525" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1722403497596" checkinUser="felipe" docAuthor="Han, Joo Hyun, Maeng, Soohyun, Park, Yuna, Lee, Sang Eun, Lee, Byoung-Hee &amp; Kim, Ki-eun Lee and Myung Kyum" docDate="2020" docId="5E1987B87705550B2858A3FC24E3F8E8" docLanguage="en" docName="JSpeciesRes.9.2.85-104.pdf" docOrigin="Journal of Species Research 9 (2)" docStyle="DocumentStyle:4D398BFF45FF8CA9E08F546BB06AE372.2:JSpeciesRes.2012-.journal_article" docStyleId="4D398BFF45FF8CA9E08F546BB06AE372" docStyleName="JSpeciesRes.2012-.journal_article" docStyleVersion="2" docTitle="Caballeronia cordobensis BT" docType="treatment" docVersion="2" lastPageNumber="89" masterDocId="A220FFC07707550F2B1FA51B2773FFF7" masterDocTitle="Identification of 12 radiation-resistant bacterial species in the phylum Proteobacteria new to Korea" masterLastPageNumber="104" masterPageNumber="85" pageNumber="87" updateTime="1722403955945" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:title id="AAA1860405988D3C964B818445F83637">Identification of 12 radiation-resistant bacterial species in the phylum Proteobacteria new to Korea</mods:title>
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<mods:namePart id="7C85C3296EFE2769024AB41C269AEB89">Han, Joo Hyun</mods:namePart>
<mods:affiliation id="973CD114997C85DCFA93FF8E352723C8">Department of Bio &amp; Environmental Technology, College of Natural Science, Seoul Womens University, Seoul 01797, Republic of Korea</mods:affiliation>
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<mods:namePart id="B3EB885F9BB3F3653E29E5343102283A">Maeng, Soohyun</mods:namePart>
<mods:affiliation id="AEE6BA61A465F2275A00A0074AB04F4F">Department of Bio &amp; Environmental Technology, College of Natural Science, Seoul Womens University, Seoul 01797, Republic of Korea</mods:affiliation>
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<mods:namePart id="3F6340A649F25F6B3DCCCCE502886B92">Park, Yuna</mods:namePart>
<mods:affiliation id="E4740712EC34851CCD49F97E1BFAB599">Department of Bio &amp; Environmental Technology, College of Natural Science, Seoul Womens University, Seoul 01797, Republic of Korea</mods:affiliation>
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<mods:namePart id="92D207DB65FB4D83E03E07AC3AB95449">Lee, Sang Eun</mods:namePart>
<mods:affiliation id="35F4A3D66C21013FFCFF2BB50B0A356B">Department of Bio &amp; Environmental Technology, College of Natural Science, Seoul Womens University, Seoul 01797, Republic of Korea</mods:affiliation>
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<mods:namePart id="8005B4E28636C57F36BD46302BC79B3D">Lee, Byoung-Hee</mods:namePart>
<mods:affiliation id="3830697AF7F8EA0B1BD9306C9B4125B4">Environmental Research Complex, National Institute of Biological Resources, Incheon 22689, Republic of Korea</mods:affiliation>
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<mods:namePart id="CE40A75A0455D0C836ACAD2DCCFA73A1">Kim, Ki-eun Lee and Myung Kyum</mods:namePart>
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<mods:date id="89FC1B049DD2C5AF0E54242FA4737B35">2020</mods:date>
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<treatment id="5E1987B87705550B2858A3FC24E3F8E8" ID-DOI="http://doi.org/10.5281/zenodo.13140517" ID-Zenodo-Dep="13140517" LSID="urn:lsid:plazi:treatment:5E1987B87705550B2858A3FC24E3F8E8" httpUri="http://treatment.plazi.org/id/5E1987B87705550B2858A3FC24E3F8E8" lastPageId="4" lastPageNumber="89" pageId="2" pageNumber="87">
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<paragraph id="D60F36AE7705550D2858A3FC222AF908" blockId="2.[839,1369,1767,1791]" box="[839,1369,1767,1791]" pageId="2" pageNumber="87">
<heading id="8D4781C27705550D2858A3FC222AF908" box="[839,1369,1767,1791]" fontSize="10" level="2" pageId="2" pageNumber="87" reason="5">
<emphasis id="E4C4EABC7705550D2858A3FC222AF908" bold="true" box="[839,1369,1767,1791]" pageId="2" pageNumber="87">
Description of
<taxonomicName id="11B04D2D7705550D28F1A3FC2242F908" authority="BT" authorityName="BT" box="[1006,1329,1767,1791]" class="Gammaproteobacteria" family="Burkholderiaceae" genus="Caballeronia" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Burkholderiales" pageId="2" pageNumber="87" phylum="Proteobacteria" rank="species" species="cordobensis">
<emphasis id="E4C4EABC7705550D28F1A3FC2274F908" bold="true" box="[1006,1287,1767,1791]" italics="true" pageId="2" pageNumber="87">Caballeronia cordobensis</emphasis>
BT
</taxonomicName>
434
</emphasis>
</heading>
</paragraph>
<paragraph id="D60F36AE7705550B2841A2332249F948" blockId="2.[839,1444,1832,1985]" lastBlockId="4.[839,1444,1573,1823]" lastPageId="4" lastPageNumber="89" pageId="2" pageNumber="87">
Cells are Gram-stain-negative, non-flagellated, and cocci-shaped. Colonies are white-colored after 3 days of incubation on R2A at 25°C. In the BIOLOG GEN III, positive for pH 6, 1% NaCl,
<emphasis id="E4C4EABC7705550D2F8FA29223D3F857" box="[1168,1184,1929,1952]" italics="true" pageId="2" pageNumber="87">α</emphasis>
-
<subScript id="4A3434EB7705550D2FB5A29423CCF856" attach="none" box="[1194,1215,1929,1953]" fontSize="7" pageId="2" pageNumber="87">D-</subScript>
glucose,
<subScript id="4A3434EB7705550D2E3AA2942249F857" attach="right" box="[1317,1338,1929,1952]" fontSize="7" pageId="2" pageNumber="87">D-</subScript>
mannose, D- fructose, D- galactose, L- rhamnose, 1% sodium lactate, myo-inositol, D- glucose-6-PO
<subScript id="4A3434EB7704550C2ACAA32D26ADF9B0" attach="right" box="[469,478,1590,1607]" fontSize="7" pageId="3" pageNumber="88">4</subScript>
, D- fructose-6-PO
<subScript id="4A3434EB7704550C2981A32D25D4F9B0" attach="left" box="[670,679,1590,1607]" fontSize="7" pageId="3" pageNumber="88">4</subScript>
, D- aspartic acid, lincomycin, D- galacturonic acid, D- glucuronic acid, mucic acid, quinic acid, D- saccharic acid, tetrazolium violet, tetrazolium blue,
<emphasis id="E4C4EABC7704550C2ADFA39426BCF950" box="[448,463,1679,1703]" italics="true" pageId="3" pageNumber="88">β</emphasis>
-hydroxy-D, L- butyric acid, propionic acid and acetic acid; weak positive for pH 5,
<emphasis id="E4C4EABC7704550C2B8FA3CA27D1F91F" box="[144,162,1745,1768]" italics="true" pageId="3" pageNumber="88">N</emphasis>
-acetyl-D- glucosamine, L- fucose, D- sorbitol, D- mannitol, D- arabitol, rifamycin SV, glyctl-L- proline, L- arginine, L- aspartic acid, L- glutamic acid, L- histidine, L- pyroglutamic acid, guanidine HCl, L- galactonic acid lactone, D- gluconic acid, vancomycin,
<emphasis id="E4C4EABC7704550C2A4CA2492613F89E" box="[339,352,1874,1897]" italics="true" pageId="3" pageNumber="88">p</emphasis>
-hydroxy-pheylacetic acid, methyl pyruvate, L- lactic acid, citric acid, L- malic acid, bromo-succinic acid, potassium tellurite, tween 40,
<emphasis id="E4C4EABC7704550C2926A289253BF85E" box="[569,584,1938,1961]" italics="true" pageId="3" pageNumber="88">γ</emphasis>
-amino-butryric acid,
<emphasis id="E4C4EABC7704550C2BD6A2A927AAF83E" box="[201,217,1970,1993]" italics="true" pageId="3" pageNumber="88">α</emphasis>
-keto butyric acid and aztreonam; but negative for dextrin, D- maltose, D- trehalose, D- cellobiolose, gentiobiose, sucrose,
<subScript id="4A3434EB7704550C28A4A34E24BDF991" attach="right" box="[955,974,1615,1638]" fontSize="7" pageId="3" pageNumber="88">D-</subScript>
turanose, stachyose,
<subScript id="4A3434EB7704550C2FB0A34E23B1F990" attach="right" box="[1199,1218,1615,1639]" fontSize="7" pageId="3" pageNumber="88">D-</subScript>
raffinose,
<emphasis id="E4C4EABC7704550C2E30A354224CF991" box="[1327,1343,1615,1638]" italics="true" pageId="3" pageNumber="88">α</emphasis>
-
<subScript id="4A3434EB7704550C2E57A34E222FF990" attach="none" box="[1352,1372,1615,1639]" fontSize="7" pageId="3" pageNumber="88">D-</subScript>
lactose, D- melibiose,
<emphasis id="E4C4EABC7704550C28F6A374248BF970" box="[1001,1016,1647,1671]" italics="true" pageId="3" pageNumber="88">β</emphasis>
-methyl-D- glucoside, D- salicin,
<emphasis id="E4C4EABC7704550C2E5FA36B2221F970" box="[1344,1362,1648,1671]" italics="true" pageId="3" pageNumber="88">N</emphasis>
-acetyl-
<emphasis id="E4C4EABC7704550C284FA394242CF950" box="[848,863,1679,1703]" italics="true" pageId="3" pageNumber="88">β</emphasis>
-mannosamin,
<emphasis id="E4C4EABC7704550C2F19A38B236BF950" box="[1030,1048,1680,1703]" italics="true" pageId="3" pageNumber="88">N</emphasis>
-acetyl-D- galactosamin,
<emphasis id="E4C4EABC7704550C2E37A38B2249F950" box="[1320,1338,1680,1703]" italics="true" pageId="3" pageNumber="88">N</emphasis>
-acetyl neuraminic acid, 4% NaCl, 8% NaCl, 3-methyl-glucose,
<subScript id="4A3434EB7704550C2835A3CD244EF91F" attach="none" box="[810,829,1744,1768]" fontSize="7" pageId="3" pageNumber="88">D-</subScript>
fucose, inosine, fusidic acid,
<subScript id="4A3434EB7704550C2F61A3CD23E2F91F" attach="right" box="[1150,1169,1744,1768]" fontSize="7" pageId="3" pageNumber="88">D-</subScript>
serine, glycerol,
<subScript id="4A3434EB7704550C2E55A3CD222FF910" attach="right" box="[1354,1372,1744,1767]" fontSize="7" pageId="3" pageNumber="88">D-</subScript>
serine, troleandomycin, minocycline, gelatin, L- alanine, L- serine, niaproof 4, pectin, glucuronamide, D- lactic acid methyl ester,
<emphasis id="E4C4EABC7704550C28A7A22A24BBF8BF" box="[952,968,1841,1864]" italics="true" pageId="3" pageNumber="88">α</emphasis>
-keto-glutaric acid,
<subScript id="4A3434EB7704550C2F86A22C23DFF8BE" attach="right" box="[1177,1196,1841,1865]" fontSize="7" pageId="3" pageNumber="88">D-</subScript>
malic acid, nalidixic acid, lithium chloride,
<emphasis id="E4C4EABC7704550C2F03A249235FF89E" box="[1052,1068,1874,1897]" italics="true" pageId="3" pageNumber="88">α</emphasis>
-hydroxy-butyric acid, acetoacetic acid, formic acid, sodium butyrate and sodium bromate. In API 20NE test, positive for reduction of nitrates (NO
<subScript id="4A3434EB7704550C2848A2A1242CF83C" attach="left" box="[855,863,1978,1995]" fontSize="7" pageId="3" pageNumber="88">3</subScript>
) to nitrite (NO
<subScript id="4A3434EB7704550C28E0A2A1237BF83C" attach="left" box="[1023,1032,1978,1995]" fontSize="7" pageId="3" pageNumber="88">2</subScript>
<superScript id="21C59BE67704550C2F18A2AB2362F84A" attach="right" box="[1031,1041,1968,1981]" fontSize="7" pageId="3" pageNumber="88">-</superScript>
) and reduction of nitrates (NO
<subScript id="4A3434EB7704550C2E47A2A12213F83C" attach="right" box="[1368,1376,1978,1995]" fontSize="7" pageId="3" pageNumber="88">3</subScript>
) to nitrogen (N
<subScript id="4A3434EB7703550B2A3BA336265FF9C9" attach="left" box="[292,300,1581,1598]" fontSize="7" pageId="4" pageNumber="89">2</subScript>
); weak positive for urease, esculin hydrolysis and gelatin hydrolysis; but negative for indole production on tryptophan, glucose fermentation, arginine dihydrolase and
<emphasis id="E4C4EABC7703550B2BC3A39D2798F969" box="[220,235,1670,1694]" italics="true" pageId="4" pageNumber="89">β</emphasis>
-galactosidase. D- Glucose, L- arabinose, D- mannose, D- mannitol,
<emphasis id="E4C4EABC7703550B2A27A3BC2639F949" box="[312,330,1703,1726]" italics="true" pageId="4" pageNumber="89">N</emphasis>
-acetyl-D- glucosamine, malic acid, phenylacetic acid (weak), potassium gluconate (weak) and trisodium citrate (weak) were utilized whereas, D- maltose, capric acid and adipic acid were not utilized. In API 32GN test, L- rhamnose,
<emphasis id="E4C4EABC7703550B2AA6A23326B8F8C8" box="[441,459,1832,1855]" italics="true" pageId="4" pageNumber="89">N</emphasis>
-acetyl-glucosamine, inositol, sodium acetate, lactic acid,
<subScript id="4A3434EB7703550B2AC3A255269EF897" attach="right" box="[476,493,1864,1888]" fontSize="7" pageId="4" pageNumber="89">L-</subScript>
alanine, potassium 5-ketogluconate, 3-hydroxybenzoic acid, L- serine, D- mannitol, D- glucose, L- arabinose, propionic acid, L- histidine, potassium 2-ketogluconate, 3-hydroxybutyric acid, 4-hydroxybenzoic acid and L- proline were utilized; D- ribose, D- saccharose (sucrose), D- maltose, itaconic acid, suberic acid, sodium malonate, D- fucose, D- sorbitol, capric acid, valeric acid and trisodium citrate were weak utilized whereas, glycogen, salicin and D- melibiose were utilized.
</paragraph>
</subSubSection>
<caption id="82CF66267704550C2B8FA086240DFA1F" ID-DOI="http://doi.org/10.5281/zenodo.13140529" ID-Zenodo-Dep="13140529" httpUri="https://zenodo.org/record/13140529/files/figure.png" pageId="3" pageNumber="88" startId="3.[144,177,1437,1459]" targetBox="[366,1194,259,1401]" targetPageId="3" targetType="figure">
<paragraph id="D60F36AE7704550C2B8FA086240DFA1F" blockId="3.[143,1413,1437,1512]" pageId="3" pageNumber="88">
<emphasis id="E4C4EABC7704550C2B8FA08627BDFA45" bold="true" box="[144,206,1437,1459]" pageId="3" pageNumber="88">Fig. 2.</emphasis>
Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences shows the relationship between the strains isolated in this study and their relatives of the species
<taxonomicName id="11B04D2D7704550C2AE8A0A32590FA3A" box="[503,739,1464,1485]" class="Gammaproteobacteria" family="Burkholderiaceae" genus="Caballeronia" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Burkholderiales" pageId="3" pageNumber="88" phylum="Proteobacteria" rank="species" species="cordobensis">
<emphasis id="E4C4EABC7704550C2AE8A0A32590FA3A" box="[503,739,1464,1485]" italics="true" pageId="3" pageNumber="88">Caballeronia cordobensis</emphasis>
</taxonomicName>
. Bootstrap values (&gt;70%) are shown above nodes for the neighbor-joining methods. Bar: 0.01 and 0.02 substitutions per nucleotide position, respectively.
</paragraph>
</caption>
<caption id="82CF66267703550B2BB1A08F236BFA28" ID-DOI="http://doi.org/10.5281/zenodo.13140531" ID-Zenodo-Dep="13140531" httpUri="https://zenodo.org/record/13140531/files/figure.png" pageId="4" pageNumber="89" startId="4.[174,207,1428,1450]" targetBox="[386,1243,259,1399]" targetPageId="4" targetType="figure">
<paragraph id="D60F36AE7703550B2BB1A08F236BFA28" blockId="4.[174,1444,1428,1503]" pageId="4" pageNumber="89">
<emphasis id="E4C4EABC7703550B2BB1A08F2798FA5E" bold="true" box="[174,235,1428,1450]" pageId="4" pageNumber="89">Fig. 3.</emphasis>
Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences shows the relationship between the strains isolated in this study and their relatives of the species
<taxonomicName id="11B04D2D7703550B293BA0B52404FA34" box="[548,887,1454,1475]" class="Gammaproteobacteria" family="Burkholderiaceae" genus="Paraburkholderia" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Burkholderiales" pageId="4" pageNumber="89" phylum="Proteobacteria" rank="species" species="kirstenboschensis">
<emphasis id="E4C4EABC7703550B293BA0B52404FA34" box="[548,887,1454,1475]" italics="true" pageId="4" pageNumber="89">Paraburkholderia kirstenboschensis</emphasis>
</taxonomicName>
. Bootstrap values (&gt;70%) are shown above nodes for the neighbor-joining methods. Bar: 0.01 and 0.02 substitutions per nucleotide position, respectively.
</paragraph>
</caption>
<subSubSection id="9EAA65257703550B2841A3DC24E3F8E8" pageId="4" pageNumber="89" type="description">
<paragraph id="D60F36AE7703550B2841A3DC24E3F8E8" blockId="4.[839,1444,1573,1823]" pageId="4" pageNumber="89">
Strain BT434 (
<emphasis id="E4C4EABC7703550B2F1BA3DD236BF929" bold="true" box="[1028,1048,1734,1758]" pageId="4" pageNumber="89">=</emphasis>
NIBRBAC000503050) was isolated from a soil sample, Ganghwa Island in
<collectingRegion id="1474F84C7703550B2E13A3FC2216F908" box="[1292,1381,1767,1791]" country="South Korea" name="Incheon" pageId="4" pageNumber="89">Incheon</collectingRegion>
City,
<collectingCountry id="AEA7763E7703550B2858A21324FFF8E8" box="[839,908,1800,1823]" name="South Korea" pageId="4" pageNumber="89">Korea</collectingCountry>
.
</paragraph>
</subSubSection>
</treatment>
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