Add updates up until 2024-12-06 14:59:30

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ggserver 2024-12-06 15:05:57 +00:00
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@ -157,8 +157,10 @@ Verhoeff, 1908
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<emphasis id="8BFFEAF4B7240E398BB5E3E4FE964D97" box="[189,308,475,501]" italics="true" pageId="37" pageNumber="38">Armadillo</emphasis>
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<emphasis id="8BFFEAF4B7240E398A33E3E4FE0E4D97" box="[315,428,475,501]" italics="true" pageId="37" pageNumber="38">tuberosus</emphasis>
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@ -201,18 +201,18 @@ Campos-Filho
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@ -236,7 +236,7 @@ leg.;
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<location id="BC54603DB7240E398A88E163FDED4F17" LSID="urn:lsid:plazi:treatment:312287F0B7240E39892AE34EFB7E48E3:BC54603DB7240E398A88E163FDED4F17" box="[384,591,859,886]" country="Colombia" latitude="10.179698" longLatPrecision="1" longitude="-75.729645" name="Casa Sra. Miriam" pageId="37" pageNumber="38" stateProvince="Bolivar, Cartagena de Indias, Islas del Rosario, Isla Grande">Casa Sra. Miriam</location>
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@ -278,9 +278,9 @@ leg.;
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<location id="BC54603DB7240E398971E1BEFD5C4FF9" LSID="urn:lsid:plazi:treatment:312287F0B7240E39892AE34EFB7E48E3:BC54603DB7240E398971E1BEFD5C4FF9" box="[633,766,897,923]" country="Colombia" latitude="10.175973" longLatPrecision="1" longitude="-75.7408" name="El Silencio" pageId="37" pageNumber="38" stateProvince="Bolivar, Cartagena de Indias, Islas del Rosario, Isla Grande">El Silencio</location>
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<location id="BC54603DB7240E398803E1BDFC594FF9" LSID="urn:lsid:plazi:treatment:312287F0B7240E39892AE34EFB074B8A:BC54603DB7240E398803E1BDFC594FF9" box="[779,1019,897,924]" country="Colombia" latitude="10.175973" longLatPrecision="1" longitude="-75.7408" name="Laguna del Silencio" pageId="37" pageNumber="38" stateProvince="Bolivar, Cartagena de Indias, Islas del Rosario, Isla Grande">Laguna del Silencio</location>
<location id="BC54603DB7240E398803E1BDFC594FF9" LSID="urn:lsid:plazi:treatment:312287F0B7240E39892AE34EFB7E48E3:BC54603DB7240E398803E1BDFC594FF9" box="[779,1019,897,924]" country="Colombia" latitude="10.175973" longLatPrecision="1" longitude="-75.7408" name="Laguna del Silencio" pageId="37" pageNumber="38" stateProvince="Bolivar, Cartagena de Indias, Islas del Rosario, Isla Grande">Laguna del Silencio</location>
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@ -340,136 +340,5 @@ Campos-Filho
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<emphasis id="8BFFEAF4B7240E398BB5E693FC1248AB" bold="true" box="[189,944,1196,1225]" italics="true" pageId="37" pageNumber="38">
Molecular phylogenetic analyses of
<taxonomicName id="7E8B4D65B7240E398995E693FC1248AB" authority="Audouin, 1826" authorityName="Audouin" authorityYear="1826" box="[669,944,1196,1225]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="genus">Tylos Audouin, 1826</taxonomicName>
</emphasis>
</paragraph>
<paragraph id="B93436E6B7240E398BB5E6DBFC684A15" blockId="37.[188,1400,1251,1655]" pageId="37" pageNumber="38">
Sequences obtained for the three species of
<taxonomicName id="7E8B4D65B7240E3989D2E6DCFCBA489F" authorityName="Audouin" authorityYear="1826" box="[730,792,1251,1277]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="genus">
<emphasis id="8BFFEAF4B7240E3989D2E6DCFCBA489F" box="[730,792,1251,1277]" italics="true" pageId="37" pageNumber="38">Tylos</emphasis>
</taxonomicName>
from
<collectingCountry id="C19C7676B7240E398860E6DCFC30489F" box="[872,914,1251,1277]" name="Iceland" pageId="37" pageNumber="38">Isla</collectingCountry>
Grande and the specimen from Florida have been deposited under GenBank Accession Numbers
<accessionNumber id="A6D8AB05B7240E39887BE736FBA24946" box="[883,1024,1289,1316]" httpUri="https://www.ncbi.nlm.nih.gov/nuccore/MW532964" pageId="37" pageNumber="38" type="GenBank">MW532964</accessionNumber>
<accessionNumber id="A6D8AB05B7240E398F07E736FB3F4946" box="[1039,1181,1289,1316]" httpUri="https://www.ncbi.nlm.nih.gov/nuccore/MW532970" pageId="37" pageNumber="38" type="GenBank">MW532970</accessionNumber>
and
<accessionNumber id="A6D8AB05B7240E398FD2E736FACA4946" box="[1242,1384,1289,1316]" httpUri="https://www.ncbi.nlm.nih.gov/nuccore/MW533069" pageId="37" pageNumber="38" type="GenBank">MW533069</accessionNumber>
<accessionNumber id="A6D8AB05B7240E398BB5E710FEED4928" box="[189,335,1327,1354]" httpUri="https://www.ncbi.nlm.nih.gov/nuccore/MW533075" pageId="37" pageNumber="38" type="GenBank">MW533075</accessionNumber>
. Only the 12S rDNA sequence was obtained for the specimen from
<collectingCountry id="C19C7676B7240E398F6DE710FB2D492B" box="[1125,1167,1327,1353]" name="Iceland" pageId="37" pageNumber="38">Isla</collectingCountry>
Grande assigned to
<taxonomicName id="7E8B4D65B7240E398BB5E76AFEEE490C" authorityName="Giordani Soika" authorityYear="1954" box="[189,332,1365,1390]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="marcuzzii">
<emphasis id="8BFFEAF4B7240E398BB5E76AFEEE490C" box="[189,332,1365,1390]" italics="true" pageId="37" pageNumber="38">T. marcuzzii</emphasis>
</taxonomicName>
. Phylogenetic analyses using only 12S rDNA group this sequence with the
<specimenCount id="AF8DFD6FB7240E398FC1E76AFAD4490D" box="[1225,1398,1365,1391]" pageId="37" pageNumber="38" type="generic">two specimens</specimenCount>
of
<taxonomicName id="7E8B4D65B7240E398BD7E744FED349F6" authorityName="Giordani Soika" authorityYear="1954" box="[223,369,1403,1428]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="marcuzzii">
<emphasis id="8BFFEAF4B7240E398BD7E744FED349F6" box="[223,369,1403,1428]" italics="true" pageId="37" pageNumber="38">T. marcuzzii</emphasis>
</taxonomicName>
from
<collectingCountry id="C19C7676B7240E398AB7E745FE5F49F6" box="[447,509,1402,1428]" name="Cuba" pageId="37" pageNumber="38">Cuba</collectingCountry>
reported in
<bibRefCitation id="DD1A4B17B7240E39899CE745FC3A49F6" author="Hurtado L. A. &amp; Lee E. J. &amp; Mateos M. &amp; Taiti S." box="[660,920,1402,1428]" pageId="37" pageNumber="38" pagination="94081" refId="ref22475" refString="Hurtado L. A., Lee E. J., Mateos M. &amp; Taiti S. 2014. Global diversification at the harsh sea-land interface: mitochondrial phylogeny of the supralittoral isopod genus Tylos (Tylidae, Oniscidea). PloS ONE 9 (4): e 94081. https: // doi. org / 10.1371 / journal. pone. 0094081" type="journal article" year="2014">
Hurtado
<emphasis id="8BFFEAF4B7240E3989F6E744FC9E49F6" box="[766,828,1402,1428]" italics="true" pageId="37" pageNumber="38">et al.</emphasis>
(2014)
</bibRefCitation>
; see
<figureCitation id="21B02A63B7240E3988DEE745FB8D49F6" box="[982,1071,1402,1428]" captionStart="Fig" captionStartId="38.[189,232,1719,1745]" captionTargetBox="[199,1386,815,1675]" captionTargetId="figure-147@38.[189,1398,810,1692]" captionTargetPageId="38" captionText="Fig. 18. Phylogenetic relationships among lineages of Tylos Audouin, 1826 from Isla Grande and their closest relatives. Majority rule consensus tree of the Bayesian posterior distribution (GTR+G+I model; 2 runs; 4 million generations; sampled every 1000; 10% burn-in), based on the dataset combining the 12S rDNA and 16S rDNA genes (Supplementary file 3). Nodes with less than 70% support (posterior probability, bootstrap, and SH-aLRT) for any dataset have been collapsed. Dashed lines represent relationships redrawn based on single gene analyses (Supplementary file 1 and Supplementary file 2). The split between the outgroup (T. marcuzzii) and the ingroup received 100% support in all analyses. Specimens from Isla Grande, Colombia are marked with a star. GenBank accession numbers for newly generated sequences are provided in parentheses." figureDoi="http://doi.org/10.5281/zenodo.6037954" httpUri="https://zenodo.org/record/6037954/files/figure.png" pageId="37" pageNumber="38">Fig. 18</figureCitation>
. The 12S rDNA p-distance between
<taxonomicName id="7E8B4D65B7240E398A2FE79EFE1549D8" authorityName="Giordani Soika" authorityYear="1954" box="[295,439,1441,1466]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="marcuzzii">
<emphasis id="8BFFEAF4B7240E398A2FE79EFE1549D8" box="[295,439,1441,1466]" italics="true" pageId="37" pageNumber="38">T. marcuzzii</emphasis>
</taxonomicName>
from
<collectingCountry id="C19C7676B7240E398909E79FFD8949D8" box="[513,555,1440,1466]" name="Iceland" pageId="37" pageNumber="38">Isla</collectingCountry>
Grande and
<taxonomicName id="7E8B4D65B7240E3989CEE79EFCF549D8" authorityName="Giordani Soika" authorityYear="1954" box="[710,855,1441,1466]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="marcuzzii">
<emphasis id="8BFFEAF4B7240E3989CEE79EFCF549D8" box="[710,855,1441,1466]" italics="true" pageId="37" pageNumber="38">T. marcuzzii</emphasis>
</taxonomicName>
from
<collectingCountry id="C19C7676B7240E3988A8E79FFC7C49D8" box="[928,990,1440,1466]" name="Cuba" pageId="37" pageNumber="38">Cuba</collectingCountry>
is 2.02.7%, whereas the distance between the specimens from the two Cuban localities is 2.0% (
<tableCitation id="F409035DB7240E3988AEE7F9FBA34982" box="[934,1025,1478,1504]" captionStart="Table 2" captionStartId="38.[189,259,265,291]" captionTargetPageId="38" captionText="Table 2. Genetic p-distance for 16S rDNA (above the diagonal) and 12S rDNA (below the diagonal) between the main genetic lineages of Tylos Audouin, 1826 in the Caribbean and Atlantic." httpUri="http://table.plazi.org/id/EDF4666EB7270E3A8BB5E336FB734D24" pageId="37" pageNumber="38" tableUuid="EDF4666EB7270E3A8BB5E336FB734D24">Table 2</tableCitation>
). Only the 16S rDNA sequence was obtained for the specimen of
<taxonomicName id="7E8B4D65B7240E398955E7D3FD6B4A67" authorityName="Budde-Lund" authorityYear="1885" box="[605,713,1516,1541]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="niveus">
<emphasis id="8BFFEAF4B7240E398955E7D3FD6B4A67" box="[605,713,1516,1541]" italics="true" pageId="37" pageNumber="38">T. niveus</emphasis>
</taxonomicName>
from the Florida Keys, the
<typeStatus id="66308844B7240E398F2AE7D3FBF74A64" box="[1058,1109,1516,1542]" pageId="37" pageNumber="38">type</typeStatus>
locality of this species. Phylogenetic analyses of 16S rDNA show that specimens identified as
<taxonomicName id="7E8B4D65B7240E398F03E42DFBD44A49" authorityName="Budde-Lund" authorityYear="1885" box="[1035,1142,1554,1579]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="niveus">
<emphasis id="8BFFEAF4B7240E398F03E42DFBD44A49" box="[1035,1142,1554,1579]" italics="true" pageId="37" pageNumber="38">T. niveus</emphasis>
</taxonomicName>
from
<collectingCountry id="C19C7676B7240E398FC8E42EFB484A49" box="[1216,1258,1553,1579]" name="Iceland" pageId="37" pageNumber="38">Isla</collectingCountry>
Grande are most closely related to the
<taxonomicName id="7E8B4D65B7240E398AF8E407FDFA4A33" authorityName="Budde-Lund" authorityYear="1885" box="[496,600,1592,1617]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="niveus">
<emphasis id="8BFFEAF4B7240E398AF8E407FDFA4A33" box="[496,600,1592,1617]" italics="true" pageId="37" pageNumber="38">T. niveus</emphasis>
</taxonomicName>
specimen from the Florida Keys. The 16S rDNA p-distance between
<taxonomicName id="7E8B4D65B7240E398BB4E462FE874A14" authorityName="Budde-Lund" authorityYear="1885" box="[188,293,1629,1654]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="niveus">
<emphasis id="8BFFEAF4B7240E398BB4E462FE874A14" box="[188,293,1629,1654]" italics="true" pageId="37" pageNumber="38">T. niveus</emphasis>
</taxonomicName>
from
<collectingCountry id="C19C7676B7240E398A66E462FE3A4A15" box="[366,408,1629,1655]" name="Iceland" pageId="37" pageNumber="38">Isla</collectingCountry>
Grande and the Florida Keys is 2.7% (
<tableCitation id="F409035DB7240E398869E462FC1E4A15" box="[865,956,1629,1655]" captionStart="Table 2" captionStartId="38.[189,259,265,291]" captionTargetPageId="38" captionText="Table 2. Genetic p-distance for 16S rDNA (above the diagonal) and 12S rDNA (below the diagonal) between the main genetic lineages of Tylos Audouin, 1826 in the Caribbean and Atlantic." httpUri="http://table.plazi.org/id/EDF4666EB7270E3A8BB5E336FB734D24" pageId="37" pageNumber="38" tableUuid="EDF4666EB7270E3A8BB5E336FB734D24">Table 2</tableCitation>
).
</paragraph>
<paragraph id="B93436E6B7240E398BB5E49FFB074B8A" blockId="37.[188,1399,1696,2025]" pageId="37" pageNumber="38">
The concatenated (12S rDNA and 16S rDNA) dataset was comprised of 43 taxa and 740 characters (excluding characters with ambiguous homology), 236 of which were parsimony-informative. The specimens assigned to
<taxonomicName id="7E8B4D65B7240E398AC7E4D2FDC24B64" authorityName="Giordani Soika" authorityYear="1954" box="[463,608,1773,1798]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="marcuzzii">
<emphasis id="8BFFEAF4B7240E398AC7E4D2FDC24B64" box="[463,608,1773,1798]" italics="true" pageId="37" pageNumber="38">T. marcuzzii</emphasis>
</taxonomicName>
were used as outgroup. Five main lineages were identified for the ingroup taxa in the concatenated phylogenetic analyses, which formed a basal polytomy (
<figureCitation id="21B02A63B7240E398E04E52DFACA4B4E" box="[1292,1384,1810,1836]" captionStart="Fig" captionStartId="38.[189,232,1719,1745]" captionTargetBox="[199,1386,815,1675]" captionTargetId="figure-147@38.[189,1398,810,1692]" captionTargetPageId="38" captionText="Fig. 18. Phylogenetic relationships among lineages of Tylos Audouin, 1826 from Isla Grande and their closest relatives. Majority rule consensus tree of the Bayesian posterior distribution (GTR+G+I model; 2 runs; 4 million generations; sampled every 1000; 10% burn-in), based on the dataset combining the 12S rDNA and 16S rDNA genes (Supplementary file 3). Nodes with less than 70% support (posterior probability, bootstrap, and SH-aLRT) for any dataset have been collapsed. Dashed lines represent relationships redrawn based on single gene analyses (Supplementary file 1 and Supplementary file 2). The split between the outgroup (T. marcuzzii) and the ingroup received 100% support in all analyses. Specimens from Isla Grande, Colombia are marked with a star. GenBank accession numbers for newly generated sequences are provided in parentheses." figureDoi="http://doi.org/10.5281/zenodo.6037954" httpUri="https://zenodo.org/record/6037954/files/figure.png" pageId="37" pageNumber="38">Fig. 18</figureCitation>
): (1)
<taxonomicName id="7E8B4D65B7240E398BE1E507FEF74B33" authorityName="Budde-Lund" authorityYear="1885" box="[233,341,1848,1873]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="niveus">
<emphasis id="8BFFEAF4B7240E398BE1E507FEF74B33" box="[233,341,1848,1873]" italics="true" pageId="37" pageNumber="38">T. niveus</emphasis>
</taxonomicName>
from
<collectingCountry id="C19C7676B7240E398AAAE507FD964B33" box="[418,564,1847,1874]" name="Puerto Rico" pageId="37" pageNumber="38">Puerto Rico</collectingCountry>
; (2)
<taxonomicName id="7E8B4D65B7240E398967E508FD7A4B30" box="[623,728,1847,1874]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="undetermined">
<emphasis id="8BFFEAF4B7240E398967E508FD0F4B33" box="[623,685,1847,1873]" italics="true" pageId="37" pageNumber="38">Tylos</emphasis>
sp.
</taxonomicName>
from Yaguanabo,
<collectingCountry id="C19C7676B7240E3988B0E508FC584B33" box="[952,1018,1847,1873]" name="Cuba" pageId="37" pageNumber="38">Cuba</collectingCountry>
; (3)
<taxonomicName id="7E8B4D65B7240E398F3CE507FB024B33" authorityName="Budde-Lund" authorityYear="1885" box="[1076,1184,1848,1873]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="niveus">
<emphasis id="8BFFEAF4B7240E398F3CE507FB024B33" box="[1076,1184,1848,1873]" italics="true" pageId="37" pageNumber="38">T. niveus</emphasis>
</taxonomicName>
from Florida and
<collectingCountry id="C19C7676B7240E398BB5E562FF454B15" box="[189,231,1885,1911]" name="Iceland" pageId="37" pageNumber="38">Isla</collectingCountry>
Grande; (4)
<taxonomicName id="7E8B4D65B7240E398A8FE561FE514B15" authority="López-Orozco &amp; Carpio-Díaz &amp; Borja-Arrieta &amp; Navas-S &amp; Campos-Filho &amp; Taiti &amp; Mateos &amp; Olazaran &amp; Caballero &amp; Jotty &amp; Gómez-Estrada &amp; Hurtado, 2022" authorityName="López-Orozco &amp; Carpio-Díaz &amp; Borja-Arrieta &amp; Navas-S &amp; Campos-Filho &amp; Taiti &amp; Mateos &amp; Olazaran &amp; Caballero &amp; Jotty &amp; Gómez-Estrada &amp; Hurtado" authorityYear="2022" box="[391,499,1886,1911]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="negroi" status="sp. nov.">
<emphasis id="8BFFEAF4B7240E398A8FE561FE514B15" box="[391,499,1886,1911]" italics="true" pageId="37" pageNumber="38">T. negroi</emphasis>
</taxonomicName>
<taxonomicNameLabel id="90CC578FB7240E398AF5E561FDFF4B1A" box="[509,605,1886,1912]" pageId="37" pageNumber="38" rank="species">sp. nov.</taxonomicNameLabel>
; and (5)
<taxonomicName id="7E8B4D65B7240E3989C3E562FC244B15" authorityName="Holmes &amp; Gay" authorityYear="1909" box="[715,902,1885,1911]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" sensu="lato" species="punctatus">
<emphasis id="8BFFEAF4B7240E3989C3E562FC244B15" box="[715,902,1885,1911]" italics="true" pageId="37" pageNumber="38">Tylos punctatus</emphasis>
</taxonomicName>
<taxonomicNameLabel id="90CC578FB7240E398886E561FBA64B15" box="[910,1028,1885,1912]" pageId="37" pageNumber="38" sensu="lato">sensu lato</taxonomicNameLabel>
from the Northeastern Pacific. The range of 16S rDNA and 12S rDNA p-distances between the four basal lineages excluding
<taxonomicName id="7E8B4D65B7240E398FEEE5BBFAD14BFF" authorityName="Holmes &amp; Gay" authorityYear="1909" box="[1254,1395,1924,1949]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="punctatus">
<emphasis id="8BFFEAF4B7240E398FEEE5BBFAD14BFF" box="[1254,1395,1924,1949]" italics="true" pageId="37" pageNumber="38">T. punctatus</emphasis>
</taxonomicName>
is 11.113.6% and 9.715.5%, respectively. The range of 16S rDNA and 12S rDNA p-distances between these ingroup lineages and
<taxonomicName id="7E8B4D65B7240E398AF4E5F0FD2E4B8A" authorityName="Giordani Soika" authorityYear="1954" box="[508,652,1999,2024]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="marcuzzii">
<emphasis id="8BFFEAF4B7240E398AF4E5F0FD2E4B8A" box="[508,652,1999,2024]" italics="true" pageId="37" pageNumber="38">T. marcuzzii</emphasis>
</taxonomicName>
is 19.422.2% and 19.523.8%, respectively.
</paragraph>
</subSubSection>
</treatment>
</document>

View file

@ -0,0 +1,279 @@
<document id="C540AA4A6AD30EA2F5E9174CAD606DFC" ID-CLB-Dataset="20702" ID-DOI="10.5852/ejt.2022.793.1643" ID-GBIF-Dataset="a449750d-d375-4849-9be9-6de9a848b659" ID-ISSN="2118-9773" ID-Zenodo-Dep="6037892" ID-ZooBank="E9F1E40A-E78B-48C4-A077-124D01696B5E" IM.materialsCitations_approvedBy="juliana" IM.metadata_approvedBy="felipe" IM.tables_approvedBy="juliana" IM.taxonomicNames_approvedBy="juliana" IM.treatmentCitations_approvedBy="juliana" IM.treatments_approvedBy="juliana" checkinTime="1644507622231" checkinUser="felipe" docAuthor="López-Orozco, Carlos Mario, Carpio-Díaz, Yesenia M., Borja-Arrieta, Ricardo, Navas-S, Gabriel R., Campos-Filho, Ivanklin Soares, Taiti, Stefano, Mateos, Mariana, Olazaran, Alexandra, Caballero, Isabel C., Jotty, Karick, Gómez-Estrada, Harold &amp; Hurtado, Luis A." docDate="2022" docId="312287F0B7240E398BB5E693FB074B8A" docLanguage="en" docName="ejt-793_1-50.pdf" docOrigin="European Journal of Taxonomy 793" docSource="http://dx.doi.org/10.5852/ejt.2022.793.1643" docStyle="DocumentStyle:EF2B578F1D15862ADE45B0C07C620911.14:EJT.2018-.journal_article.type1" docStyleId="EF2B578F1D15862ADE45B0C07C620911" docStyleName="EJT.2018-.journal_article.type1" docStyleVersion="14" docTitle="Tylos Audouin 1826" docType="treatment" docVersion="1" lastPageNumber="38" masterDocId="CD1BFF88B7010E1C8B08E23FFFA24C62" masterDocTitle="A glimpse into a remarkable unknown diversity of oniscideans along the Caribbean coasts revealed on a tiny island" masterLastPageNumber="50" masterPageNumber="1" pageNumber="38" updateTime="1733497110535" updateUser="juliana" zenodo-license-document="CC-BY-4.0" zenodo-license-figures="CC-BY-4.0" zenodo-license-treatments="UNSPECIFIED">
<mods:mods id="F15F40941994EBDF181D84480684670C" xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo id="3CABFC0491143AB934433314C02938FD">
<mods:title id="AC807017D67BB68C88214B4C024B084D">A glimpse into a remarkable unknown diversity of oniscideans along the Caribbean coasts revealed on a tiny island</mods:title>
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<mods:role id="861C9FBB05F84C4DE68DB3BAB0506FBB">
<mods:roleTerm id="D689C2E96826AEFA02EF04C89CA95A80">Author</mods:roleTerm>
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<mods:namePart id="2BA7EE2BD05DFC576F6B689279A5440C">López-Orozco, Carlos Mario</mods:namePart>
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<mods:affiliation id="E310A5377C0F6AF2FF71BB2189394F24">Grupo de Investigación en Biología Descriptiva y Aplicada, Universidad de Cartagena, Programa de Biología, Campus San Pablo, Cartagena de Indias, Colombia.</mods:affiliation>
<mods:nameIdentifier id="C6F5014B353E41A293D7CA7F1A56B0BF" type="email">clopezo1@unicartagena.edu.co</mods:nameIdentifier>
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<mods:affiliation id="E1914E41F0BD0647D6818BCEEC188C7E">Department of Ecology and Conservation Biology, Texas A &amp; M University, College Station, Texas, USA.</mods:affiliation>
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<subSubSection id="F191656DB7240E398BB5E693FC1248AB" box="[189,944,1196,1225]" pageId="37" pageNumber="38" type="nomenclature">
<paragraph id="B93436E6B7240E398BB5E693FC1248AB" blockId="37.[189,944,1196,1225]" box="[189,944,1196,1225]" pageId="37" pageNumber="38">
<emphasis id="8BFFEAF4B7240E398BB5E693FC1248AB" bold="true" box="[189,944,1196,1225]" italics="true" pageId="37" pageNumber="38">
Molecular phylogenetic analyses of
<taxonomicName id="7E8B4D65B7240E398995E693FC1248AB" ID-CoL="7Q5LR" authority="Audouin, 1826" authorityName="Audouin" authorityYear="1826" box="[669,944,1196,1225]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="genus">Tylos Audouin, 1826</taxonomicName>
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<paragraph id="B93436E6B7240E398BB5E6DBFC684A15" blockId="37.[188,1400,1251,1655]" pageId="37" pageNumber="38">
Sequences obtained for the three species of
<taxonomicName id="7E8B4D65B7240E3989D2E6DCFCBA489F" authorityName="Audouin" authorityYear="1826" box="[730,792,1251,1277]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="genus">
<emphasis id="8BFFEAF4B7240E3989D2E6DCFCBA489F" box="[730,792,1251,1277]" italics="true" pageId="37" pageNumber="38">Tylos</emphasis>
</taxonomicName>
from
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Grande and the specimen from Florida have been deposited under GenBank Accession Numbers
<accessionNumber id="A6D8AB05B7240E39887BE736FBA24946" box="[883,1024,1289,1316]" httpUri="https://www.ncbi.nlm.nih.gov/nuccore/MW532964" pageId="37" pageNumber="38" type="GenBank">MW532964</accessionNumber>
<accessionNumber id="A6D8AB05B7240E398F07E736FB3F4946" box="[1039,1181,1289,1316]" httpUri="https://www.ncbi.nlm.nih.gov/nuccore/MW532970" pageId="37" pageNumber="38" type="GenBank">MW532970</accessionNumber>
and
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<accessionNumber id="A6D8AB05B7240E398BB5E710FEED4928" box="[189,335,1327,1354]" httpUri="https://www.ncbi.nlm.nih.gov/nuccore/MW533075" pageId="37" pageNumber="38" type="GenBank">MW533075</accessionNumber>
. Only the 12S rDNA sequence was obtained for the specimen from
<collectingCountry id="C19C7676B7240E398F6DE710FB2D492B" box="[1125,1167,1327,1353]" name="Iceland" pageId="37" pageNumber="38">Isla</collectingCountry>
Grande assigned to
<taxonomicName id="7E8B4D65B7240E398BB5E76AFEEE490C" authorityName="Giordani Soika" authorityYear="1954" box="[189,332,1365,1390]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="marcuzzii">
<emphasis id="8BFFEAF4B7240E398BB5E76AFEEE490C" box="[189,332,1365,1390]" italics="true" pageId="37" pageNumber="38">T. marcuzzii</emphasis>
</taxonomicName>
. Phylogenetic analyses using only 12S rDNA group this sequence with the
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of
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<emphasis id="8BFFEAF4B7240E398BD7E744FED349F6" box="[223,369,1403,1428]" italics="true" pageId="37" pageNumber="38">T. marcuzzii</emphasis>
</taxonomicName>
from
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reported in
<bibRefCitation id="DD1A4B17B7240E39899CE745FC3A49F6" author="Hurtado L. A. &amp; Lee E. J. &amp; Mateos M. &amp; Taiti S." box="[660,920,1402,1428]" pageId="37" pageNumber="38" pagination="94081" refId="ref22475" refString="Hurtado L. A., Lee E. J., Mateos M. &amp; Taiti S. 2014. Global diversification at the harsh sea-land interface: mitochondrial phylogeny of the supralittoral isopod genus Tylos (Tylidae, Oniscidea). PloS ONE 9 (4): e 94081. https: // doi. org / 10.1371 / journal. pone. 0094081" type="journal article" year="2014">
Hurtado
<emphasis id="8BFFEAF4B7240E3989F6E744FC9E49F6" box="[766,828,1402,1428]" italics="true" pageId="37" pageNumber="38">et al.</emphasis>
(2014)
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; see
<figureCitation id="21B02A63B7240E3988DEE745FB8D49F6" box="[982,1071,1402,1428]" captionStart="Fig" captionStartId="38.[189,232,1719,1745]" captionTargetBox="[199,1386,815,1675]" captionTargetId="figure-147@38.[189,1398,810,1692]" captionTargetPageId="38" captionText="Fig. 18. Phylogenetic relationships among lineages of Tylos Audouin, 1826 from Isla Grande and their closest relatives. Majority rule consensus tree of the Bayesian posterior distribution (GTR+G+I model; 2 runs; 4 million generations; sampled every 1000; 10% burn-in), based on the dataset combining the 12S rDNA and 16S rDNA genes (Supplementary file 3). Nodes with less than 70% support (posterior probability, bootstrap, and SH-aLRT) for any dataset have been collapsed. Dashed lines represent relationships redrawn based on single gene analyses (Supplementary file 1 and Supplementary file 2). The split between the outgroup (T. marcuzzii) and the ingroup received 100% support in all analyses. Specimens from Isla Grande, Colombia are marked with a star. GenBank accession numbers for newly generated sequences are provided in parentheses." figureDoi="http://doi.org/10.5281/zenodo.6037954" httpUri="https://zenodo.org/record/6037954/files/figure.png" pageId="37" pageNumber="38">Fig. 18</figureCitation>
. The 12S rDNA p-distance between
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<emphasis id="8BFFEAF4B7240E398A2FE79EFE1549D8" box="[295,439,1441,1466]" italics="true" pageId="37" pageNumber="38">T. marcuzzii</emphasis>
</taxonomicName>
from
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Grande and
<taxonomicName id="7E8B4D65B7240E3989CEE79EFCF549D8" authorityName="Giordani Soika" authorityYear="1954" box="[710,855,1441,1466]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="marcuzzii">
<emphasis id="8BFFEAF4B7240E3989CEE79EFCF549D8" box="[710,855,1441,1466]" italics="true" pageId="37" pageNumber="38">T. marcuzzii</emphasis>
</taxonomicName>
from
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is 2.02.7%, whereas the distance between the specimens from the two Cuban localities is 2.0% (
<tableCitation id="F409035DB7240E3988AEE7F9FBA34982" box="[934,1025,1478,1504]" captionStart="Table 2" captionStartId="38.[189,259,265,291]" captionTargetPageId="38" captionText="Table 2. Genetic p-distance for 16S rDNA (above the diagonal) and 12S rDNA (below the diagonal) between the main genetic lineages of Tylos Audouin, 1826 in the Caribbean and Atlantic." httpUri="http://table.plazi.org/id/EDF4666EB7270E3A8BB5E336FB734D24" pageId="37" pageNumber="38" tableUuid="EDF4666EB7270E3A8BB5E336FB734D24">Table 2</tableCitation>
). Only the 16S rDNA sequence was obtained for the specimen of
<taxonomicName id="7E8B4D65B7240E398955E7D3FD6B4A67" authorityName="Budde-Lund" authorityYear="1885" box="[605,713,1516,1541]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="niveus">
<emphasis id="8BFFEAF4B7240E398955E7D3FD6B4A67" box="[605,713,1516,1541]" italics="true" pageId="37" pageNumber="38">T. niveus</emphasis>
</taxonomicName>
from the Florida Keys, the
<typeStatus id="66308844B7240E398F2AE7D3FBF74A64" box="[1058,1109,1516,1542]" pageId="37" pageNumber="38">type</typeStatus>
locality of this species. Phylogenetic analyses of 16S rDNA show that specimens identified as
<taxonomicName id="7E8B4D65B7240E398F03E42DFBD44A49" authorityName="Budde-Lund" authorityYear="1885" box="[1035,1142,1554,1579]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="niveus">
<emphasis id="8BFFEAF4B7240E398F03E42DFBD44A49" box="[1035,1142,1554,1579]" italics="true" pageId="37" pageNumber="38">T. niveus</emphasis>
</taxonomicName>
from
<collectingCountry id="C19C7676B7240E398FC8E42EFB484A49" box="[1216,1258,1553,1579]" name="Iceland" pageId="37" pageNumber="38">Isla</collectingCountry>
Grande are most closely related to the
<taxonomicName id="7E8B4D65B7240E398AF8E407FDFA4A33" authorityName="Budde-Lund" authorityYear="1885" box="[496,600,1592,1617]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="niveus">
<emphasis id="8BFFEAF4B7240E398AF8E407FDFA4A33" box="[496,600,1592,1617]" italics="true" pageId="37" pageNumber="38">T. niveus</emphasis>
</taxonomicName>
specimen from the Florida Keys. The 16S rDNA p-distance between
<taxonomicName id="7E8B4D65B7240E398BB4E462FE874A14" authorityName="Budde-Lund" authorityYear="1885" box="[188,293,1629,1654]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="niveus">
<emphasis id="8BFFEAF4B7240E398BB4E462FE874A14" box="[188,293,1629,1654]" italics="true" pageId="37" pageNumber="38">T. niveus</emphasis>
</taxonomicName>
from
<collectingCountry id="C19C7676B7240E398A66E462FE3A4A15" box="[366,408,1629,1655]" name="Iceland" pageId="37" pageNumber="38">Isla</collectingCountry>
Grande and the Florida Keys is 2.7% (
<tableCitation id="F409035DB7240E398869E462FC1E4A15" box="[865,956,1629,1655]" captionStart="Table 2" captionStartId="38.[189,259,265,291]" captionTargetPageId="38" captionText="Table 2. Genetic p-distance for 16S rDNA (above the diagonal) and 12S rDNA (below the diagonal) between the main genetic lineages of Tylos Audouin, 1826 in the Caribbean and Atlantic." httpUri="http://table.plazi.org/id/EDF4666EB7270E3A8BB5E336FB734D24" pageId="37" pageNumber="38" tableUuid="EDF4666EB7270E3A8BB5E336FB734D24">Table 2</tableCitation>
).
</paragraph>
<paragraph id="B93436E6B7240E398BB5E49FFB074B8A" blockId="37.[188,1399,1696,2025]" pageId="37" pageNumber="38">
The concatenated (12S rDNA and 16S rDNA) dataset was comprised of 43 taxa and 740 characters (excluding characters with ambiguous homology), 236 of which were parsimony-informative. The specimens assigned to
<taxonomicName id="7E8B4D65B7240E398AC7E4D2FDC24B64" authorityName="Giordani Soika" authorityYear="1954" box="[463,608,1773,1798]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="marcuzzii">
<emphasis id="8BFFEAF4B7240E398AC7E4D2FDC24B64" box="[463,608,1773,1798]" italics="true" pageId="37" pageNumber="38">T. marcuzzii</emphasis>
</taxonomicName>
were used as outgroup. Five main lineages were identified for the ingroup taxa in the concatenated phylogenetic analyses, which formed a basal polytomy (
<figureCitation id="21B02A63B7240E398E04E52DFACA4B4E" box="[1292,1384,1810,1836]" captionStart="Fig" captionStartId="38.[189,232,1719,1745]" captionTargetBox="[199,1386,815,1675]" captionTargetId="figure-147@38.[189,1398,810,1692]" captionTargetPageId="38" captionText="Fig. 18. Phylogenetic relationships among lineages of Tylos Audouin, 1826 from Isla Grande and their closest relatives. Majority rule consensus tree of the Bayesian posterior distribution (GTR+G+I model; 2 runs; 4 million generations; sampled every 1000; 10% burn-in), based on the dataset combining the 12S rDNA and 16S rDNA genes (Supplementary file 3). Nodes with less than 70% support (posterior probability, bootstrap, and SH-aLRT) for any dataset have been collapsed. Dashed lines represent relationships redrawn based on single gene analyses (Supplementary file 1 and Supplementary file 2). The split between the outgroup (T. marcuzzii) and the ingroup received 100% support in all analyses. Specimens from Isla Grande, Colombia are marked with a star. GenBank accession numbers for newly generated sequences are provided in parentheses." figureDoi="http://doi.org/10.5281/zenodo.6037954" httpUri="https://zenodo.org/record/6037954/files/figure.png" pageId="37" pageNumber="38">Fig. 18</figureCitation>
): (1)
<taxonomicName id="7E8B4D65B7240E398BE1E507FEF74B33" authorityName="Budde-Lund" authorityYear="1885" box="[233,341,1848,1873]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="niveus">
<emphasis id="8BFFEAF4B7240E398BE1E507FEF74B33" box="[233,341,1848,1873]" italics="true" pageId="37" pageNumber="38">T. niveus</emphasis>
</taxonomicName>
from
<collectingCountry id="C19C7676B7240E398AAAE507FD964B33" box="[418,564,1847,1874]" name="Puerto Rico" pageId="37" pageNumber="38">Puerto Rico</collectingCountry>
; (2)
<taxonomicName id="7E8B4D65B7240E398967E508FD7A4B30" box="[623,728,1847,1874]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="undetermined">
<emphasis id="8BFFEAF4B7240E398967E508FD0F4B33" box="[623,685,1847,1873]" italics="true" pageId="37" pageNumber="38">Tylos</emphasis>
sp.
</taxonomicName>
from Yaguanabo,
<collectingCountry id="C19C7676B7240E3988B0E508FC584B33" box="[952,1018,1847,1873]" name="Cuba" pageId="37" pageNumber="38">Cuba</collectingCountry>
; (3)
<taxonomicName id="7E8B4D65B7240E398F3CE507FB024B33" authorityName="Budde-Lund" authorityYear="1885" box="[1076,1184,1848,1873]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="niveus">
<emphasis id="8BFFEAF4B7240E398F3CE507FB024B33" box="[1076,1184,1848,1873]" italics="true" pageId="37" pageNumber="38">T. niveus</emphasis>
</taxonomicName>
from Florida and
<collectingCountry id="C19C7676B7240E398BB5E562FF454B15" box="[189,231,1885,1911]" name="Iceland" pageId="37" pageNumber="38">Isla</collectingCountry>
Grande; (4)
<taxonomicName id="7E8B4D65B7240E398A8FE561FE514B15" authority="López-Orozco &amp; Carpio-Díaz &amp; Borja-Arrieta &amp; Navas-S &amp; Campos-Filho &amp; Taiti &amp; Mateos &amp; Olazaran &amp; Caballero &amp; Jotty &amp; Gómez-Estrada &amp; Hurtado, 2022" authorityName="López-Orozco &amp; Carpio-Díaz &amp; Borja-Arrieta &amp; Navas-S &amp; Campos-Filho &amp; Taiti &amp; Mateos &amp; Olazaran &amp; Caballero &amp; Jotty &amp; Gómez-Estrada &amp; Hurtado" authorityYear="2022" box="[391,499,1886,1911]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="negroi" status="sp. nov.">
<emphasis id="8BFFEAF4B7240E398A8FE561FE514B15" box="[391,499,1886,1911]" italics="true" pageId="37" pageNumber="38">T. negroi</emphasis>
</taxonomicName>
<taxonomicNameLabel id="90CC578FB7240E398AF5E561FDFF4B1A" box="[509,605,1886,1912]" pageId="37" pageNumber="38" rank="species">sp. nov.</taxonomicNameLabel>
; and (5)
<taxonomicName id="7E8B4D65B7240E3989C3E562FC244B15" authorityName="Holmes &amp; Gay" authorityYear="1909" box="[715,902,1885,1911]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" sensu="lato" species="punctatus">
<emphasis id="8BFFEAF4B7240E3989C3E562FC244B15" box="[715,902,1885,1911]" italics="true" pageId="37" pageNumber="38">Tylos punctatus</emphasis>
</taxonomicName>
<taxonomicNameLabel id="90CC578FB7240E398886E561FBA64B15" box="[910,1028,1885,1912]" pageId="37" pageNumber="38" sensu="lato">sensu lato</taxonomicNameLabel>
from the Northeastern Pacific. The range of 16S rDNA and 12S rDNA p-distances between the four basal lineages excluding
<taxonomicName id="7E8B4D65B7240E398FEEE5BBFAD14BFF" authorityName="Holmes &amp; Gay" authorityYear="1909" box="[1254,1395,1924,1949]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="punctatus">
<emphasis id="8BFFEAF4B7240E398FEEE5BBFAD14BFF" box="[1254,1395,1924,1949]" italics="true" pageId="37" pageNumber="38">T. punctatus</emphasis>
</taxonomicName>
is 11.113.6% and 9.715.5%, respectively. The range of 16S rDNA and 12S rDNA p-distances between these ingroup lineages and
<taxonomicName id="7E8B4D65B7240E398AF4E5F0FD2E4B8A" authorityName="Giordani Soika" authorityYear="1954" box="[508,652,1999,2024]" class="Malacostraca" family="Tylidae" genus="Tylos" kingdom="Animalia" order="Isopoda" pageId="37" pageNumber="38" phylum="Arthropoda" rank="species" species="marcuzzii">
<emphasis id="8BFFEAF4B7240E398AF4E5F0FD2E4B8A" box="[508,652,1999,2024]" italics="true" pageId="37" pageNumber="38">T. marcuzzii</emphasis>
</taxonomicName>
is 19.422.2% and 19.523.8%, respectively.
</paragraph>
</subSubSection>
</treatment>
</document>