treatments-xml/data/97/0A/87/970A878DFFAB0E12FF22FC1BCF63F8FE.xml

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<mods:title id="A1C0CC950259FBF6C5468CFB8CA0CB0C">Egg morphology of six East Palaearctic species of the genus Ephemera Linnaeus (Ephemeroptera: Ephemeridae)</mods:title>
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<paragraph id="1F1C369BFFAB0E12FF22FC1BCF62FC7F" blockId="1.[151,775,966,1030]" box="[151,775,966,993]" pageId="1" pageNumber="382">
<heading id="445481F7FFAB0E12FF22FC1BCF62FC7F" bold="true" box="[151,775,966,993]" fontSize="11" level="1" pageId="1" pageNumber="382" reason="1">
<taxonomicName id="D8A34D18FFAB0E12FF22FC1BCF62FC7F" ID-CoL="6FP7W" authority="McLachlan, 1875" authorityName="McLachlan" authorityYear="1875" box="[151,775,966,993]" class="Insecta" family="Ephemeridae" genus="Ephemera" kingdom="Animalia" order="Ephemeroptera" pageId="1" pageNumber="382" phylum="Arthropoda" rank="species" species="orientalis" subGenus="Ephemera">
<emphasis id="2DD7EA89FFAB0E12FF22FC1BCF62FC7F" bold="true" box="[151,775,966,993]" pageId="1" pageNumber="382">
<emphasis id="2DD7EA89FFAB0E12FF22FC1BCD71FC7E" bold="true" box="[151,276,966,993]" italics="true" pageId="1" pageNumber="382">Ephemera</emphasis>
(
<emphasis id="2DD7EA89FFAB0E12FE93FC1BCDC6FC7E" bold="true" box="[294,419,966,993]" italics="true" pageId="1" pageNumber="382">Ephemera</emphasis>
)
<emphasis id="2DD7EA89FFAB0E12FE01FC1BCE43FC7E" bold="true" box="[436,550,966,993]" italics="true" pageId="1" pageNumber="382">orientalis</emphasis>
<bibRefCitation id="7B324B6AFFAB0E12FD9BFC1ACF62FC7F" author="McLachlan, R." box="[558,775,966,993]" pageId="1" pageNumber="382" pagination="167 - 190" refId="ref7290" refString="McLachlan, R. (1875) A sketch of our present knowledge of the neuropterous fauna of Japan (excluding Odonata and Trichoptera). Transactions of the Entomological Society, London, 167 - 190. https: // doi. org / 10.1111 / j. 1365 - 2311.1875. tb 01906. x" type="book chapter" year="1875">McLachlan, 1875</bibRefCitation>
</emphasis>
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</heading>
</paragraph>
</subSubSection>
<subSubSection id="57B96510FFAB0E12FF22FC31CD79FB99" box="[151,284,1004,1030]" pageId="1" pageNumber="382" type="description">
<paragraph id="1F1C369BFFAB0E12FF22FC31CD79FB99" blockId="1.[151,775,966,1030]" box="[151,284,1004,1030]" pageId="1" pageNumber="382">
<figureCitation id="87982A1EFFAB0E12FF22FC31CD79FB99" box="[151,284,1004,1030]" captionStart-0="FIGURES 14" captionStart-1="FIGURES 58" captionStartId-0="2.[151,264,1943,1967]" captionStartId-1="3.[151,264,1943,1967]" captionTargetBox-0="[246,1341,181,1919]" captionTargetBox-1="[232,1355,181,1919]" captionTargetId-0="figure-18@2.[246,1341,181,1919]" captionTargetId-1="figure-16@3.[232,1355,181,1919]" captionTargetPageId-0="2" captionTargetPageId-1="3" captionText-0="FIGURES 14. Egg morphology of Ephemera (Ephemera) orientalis: 12, general shape; 34, micropyle of the complex extrachorion-adhesive layer. CM—canal micropylar; MO—micropylar opening; mp—micropyle; SG—sperm guide." captionText-1="FIGURES 58. Egg morphology of Ephemera (Ephemera) orientalis: 56, egg surface without complex extrachorion-adhesive layer; 7, proximal part of micropylar canal on chorion surface; 8, thickness of the layer forming the eggshell. AL-Ex—complex adhesive-extrachorion layer; CS—chorion surface; MO—micropylar opening; mp—micropyle." figureDoi-0="http://doi.org/10.5281/zenodo.13618717" figureDoi-1="http://doi.org/10.5281/zenodo.13618721" httpUri-0="https://zenodo.org/record/13618717/files/figure.png" httpUri-1="https://zenodo.org/record/13618721/files/figure.png" pageId="1" pageNumber="382">Figures 18</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="57B96510FFAB0E12FF22FBEBC938FB04" pageId="1" pageNumber="382" type="materials_examined">
<paragraph id="1F1C369BFFAB0E12FF22FBEBC938FB04" blockId="1.[151,1437,1078,1889]" pageId="1" pageNumber="382">
<emphasis id="2DD7EA89FFAB0E12FF22FBEBCD12FBCF" bold="true" box="[151,375,1078,1104]" pageId="1" pageNumber="382">Material examined</emphasis>
.
<materialsCitation id="AFCB3CC6FFAB0E12FE31FBEAC9F9FBCE" box="[388,1436,1079,1105]" country="Russia" location="Kabarga River" municipality="Ussuri River" pageId="1" pageNumber="382" specimenCount="1" stateProvince="Primorskiy">
<collectingCountry id="67B4760BFFAB0E12FE31FBEACDB6FBCE" box="[388,467,1079,1105]" name="Russia" pageId="1" pageNumber="382">Russia</collectingCountry>
:
<collectingRegion id="DD67F879FFAB0E12FE6EFBEACEEFFBCE" box="[475,650,1079,1105]" country="Russia" name="Primorskiy" pageId="1" pageNumber="382">Primorsky Krai</collectingRegion>
,
<collectingMunicipality id="FF78ACE1FFAB0E12FD26FBEACF7AFBCE" box="[659,799,1079,1105]" pageId="1" pageNumber="382">Ussuri River</collectingMunicipality>
basin,
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, below the road bridge, Vladivostok
</materialsCitation>
<materialsCitation id="AFCB3CC6FFAB0E12FF19FB81CD01FB04" collectingDate="2000-06-17" collectingDateMax="2005-06-15" collectingDateMin="2000-06-17" collectionCode="T" country="Russia" location="Stepanovka village" municipality="Ussuri River" pageId="1" pageNumber="382" specimenCount="5" specimenCount-female="5" stateProvince="Khabarovsk">
<collectingRegion id="DD67F879FFAB0E12FF19FB81CD56FBE9" box="[172,307,1116,1142]" country="Russia" name="Khabarovsk" pageId="1" pageNumber="382">Khabarovsk</collectingRegion>
highway,
<date id="6B1D105BFFAB0E12FE1DFB81CE4DFBE9" box="[424,552,1116,1142]" pageId="1" pageNumber="382" value="2000-06-17">
<collectingDate id="7B59E9B3FFAB0E12FE1DFB81CE4DFBE9" box="[424,552,1116,1142]" pageId="1" pageNumber="382" value="2000-06-17">17.06.2000</collectingDate>
</date>
,
<specimenCount id="09A5FD12FFAB0E12FD81FB81CE33FBE9" box="[564,598,1116,1142]" count="3" pageId="1" pageNumber="382" type="female">3♀</specimenCount>
adults,
<collectionCode id="79B2AE5EFFAB0E12FD1BFB80CEA5FBE8" box="[686,704,1117,1143]" name="Tavera, Department of Geology and Geophysics" pageId="1" pageNumber="382">T</collectionCode>
. Tiunova;
<collectingMunicipality id="FF78ACE1FFAB0E12FC83FB81CFA3FBE9" box="[822,966,1116,1142]" pageId="1" pageNumber="382">Ussuri River</collectingMunicipality>
, near
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,
<date id="6B1D105BFFAB0E12FB58FB81C90BFBE8" box="[1261,1390,1116,1143]" pageId="1" pageNumber="382" value="2005-06-15">
<collectingDate id="7B59E9B3FFAB0E12FB58FB81C90BFBE8" box="[1261,1390,1116,1143]" pageId="1" pageNumber="382" value="2005-06-15">15.06.2005</collectingDate>
</date>
,
<specimenCount id="09A5FD12FFAB0E12FACCFB81C9F9FBE9" box="[1401,1436,1116,1142]" count="2" pageId="1" pageNumber="382" type="female">2♀</specimenCount>
adults,
<collectionCode id="79B2AE5EFFAB0E12FF5DFB5FCC9FFB03" box="[232,250,1154,1180]" name="Tavera, Department of Geology and Geophysics" pageId="1" pageNumber="382">T</collectionCode>
. Tiunova
</materialsCitation>
;
<materialsCitation id="AFCB3CC6FFAB0E12FEDAFB5CC93CFB04" box="[367,1369,1153,1180]" collectingDate="2005-06-30" collectionCode="T" country="Russia" county="Khabarovsky Krai" location="Bystrinsk village" municipality="River" pageId="1" pageNumber="382" specimenCount="1" specimenCount-female="1" stateProvince="Amur">
<collectingCounty id="F67D4E17FFAB0E12FEDAFB5CCE24FB04" box="[367,577,1153,1179]" pageId="1" pageNumber="382">Khabarovsky Krai</collectingCounty>
,
<collectingRegion id="DD67F879FFAB0E12FDFFFB5FCEEEFB03" box="[586,651,1154,1180]" country="Russia" name="Amur" pageId="1" pageNumber="382">Amur</collectingRegion>
<collectingMunicipality id="FF78ACE1FFAB0E12FD27FB5CCEB7FB04" box="[658,722,1153,1179]" pageId="1" pageNumber="382">River</collectingMunicipality>
, near
<location id="1A7C6040FFAB0E12FCA1FB5CCFB3FB04" LSID="urn:lsid:plazi:treatment:970A878DFFAB0E12FF22FC1BCF63F8FE:1A7C6040FFAB0E12FCA1FB5CCFB3FB04" box="[788,982,1153,1179]" country="Russia" county="Khabarovsky Krai" municipality="River" name="Bystrinsk village" pageId="1" pageNumber="382" stateProvince="Amur">Bystrinsk village</location>
,
<date id="6B1D105BFFAB0E12FC54FB5CC807FB03" box="[993,1122,1153,1180]" pageId="1" pageNumber="382" value="2005-06-30">
<collectingDate id="7B59E9B3FFAB0E12FC54FB5CC807FB03" box="[993,1122,1153,1180]" pageId="1" pageNumber="382" value="2005-06-30">30.06.2005</collectingDate>
</date>
,
<specimenCount id="09A5FD12FFAB0E12FBD8FB5CC8F5FB04" box="[1133,1168,1153,1179]" count="1" pageId="1" pageNumber="382" type="female">1♀</specimenCount>
adult,
<collectionCode id="79B2AE5EFFAB0E12FB69FB5FC88BFB03" box="[1244,1262,1154,1180]" name="Tavera, Department of Geology and Geophysics" pageId="1" pageNumber="382">T</collectionCode>
. Tiunova
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="57B96510FFAB0E12FF72FB7BCF63F8FE" pageId="1" pageNumber="382" type="distribution">
<paragraph id="1F1C369BFFAB0E12FF72FB7BC9EAFB5E" blockId="1.[151,1437,1078,1889]" box="[199,1423,1190,1217]" pageId="1" pageNumber="382">
<emphasis id="2DD7EA89FFAB0E12FF72FB7BCD3FFB5F" box="[199,346,1190,1217]" italics="true" pageId="1" pageNumber="382">
<emphasis id="2DD7EA89FFAB0E12FF72FB7BCD36FB5E" bold="true" box="[199,339,1190,1217]" italics="true" pageId="1" pageNumber="382">Distribution</emphasis>
.
</emphasis>
Siberia, Far-East
<collectingCountry id="67B4760BFFAB0E12FD91FB7ACE17FB5E" box="[548,626,1191,1217]" name="Russia" pageId="1" pageNumber="382">Russia</collectingCountry>
,
<collectingCountry id="67B4760BFFAB0E12FDC8FB7ACEA5FB5E" box="[637,704,1191,1217]" name="Japan" pageId="1" pageNumber="382">Japan</collectingCountry>
,
<collectingCountry id="67B4760BFFAB0E12FD7FFB7ACF5FFB5E" box="[714,826,1191,1217]" name="Mongolia" pageId="1" pageNumber="382">Mongolia</collectingCountry>
,
<collectingCountry id="67B4760BFFAB0E12FCF0FB7ACFE9FB5E" box="[837,908,1191,1217]" name="South Korea" pageId="1" pageNumber="382">Korea</collectingCountry>
, N.E.
<collectingCountry id="67B4760BFFAB0E12FC67FB7AC87DFB5E" box="[978,1048,1191,1217]" name="China" pageId="1" pageNumber="382">China</collectingCountry>
,
<collectingCountry id="67B4760BFFAB0E12FB96FB7AC8C3FB5E" box="[1059,1190,1191,1217]" name="Kazakhstan" pageId="1" pageNumber="382">Kazakhstan</collectingCountry>
(Irtysh River basin).
</paragraph>
<paragraph id="1F1C369BFFAB0E12FF72FB11C97FFAE4" blockId="1.[151,1437,1078,1889]" pageId="1" pageNumber="382">
The egg has been described by
<bibRefCitation id="7B324B6AFFAB0E12FD9EFB11CEB1FB79" author="Okazaki, H." box="[555,724,1228,1254]" pageId="1" pageNumber="382" pagination="8 - 10" refId="ref7347" refString="Okazaki, H. (1981) On the eggs of Japanese mayflies (1). Biology of Inland Waters, 2, 8 - 10." type="journal article" year="1981">Okazaki (1981</bibRefCitation>
, p. 9: fig. 5; 1984, p. 21: fig. 16),
<bibRefCitation id="7B324B6AFFAB0E12FBEDFB11C971FB79" author="Su, C. - R. &amp; Zhu, C. - D." box="[1112,1300,1228,1254]" pageId="1" pageNumber="382" pagination="121 - 123" refId="ref7405" refString="Su, C. - R. &amp; Zhu, C. - D. (1997) Observation on the chorion structure of four species of mayfly by scanning electron microscope. Journal of Nanjing Normal University (Nature Science), 20 (4), 121 - 123." type="journal article" year="1997">Su &amp; Zhu (1997</bibRefCitation>
, p. 122: fig. 70), and
<bibRefCitation id="7B324B6AFFAB0E12FF4CFB2CCD98FA94" author="Tojo, K. &amp; Machida, R." box="[249,509,1265,1291]" pageId="1" pageNumber="382" pagination="573 - 579" refId="ref7457" refString="Tojo, K. &amp; Machida, R. (1998) Egg Structures of Japanes Ephemeridae species (Ephemeroptera). Entomological Science, 1 (4), 573 - 579." type="journal article" year="1998">Tojo &amp; Machida (1998</bibRefCitation>
, p. 576: fig. 3). From the above descriptions, the following can be emphasized: the egg is rectangular in shape, with a length of 187.0 µm and a width of 120.8 µm. In the work of Tojo and Machida, the egg is ellipsoidal in shape, about 200x100 µm, the adhesive layer is thick (34 µm), smooth, the chorion has well-defined reticulation, the opening diameter is 11.5 µm, and the sperm guide is hood-like in shape.
</paragraph>
<paragraph id="1F1C369BFFAB0E12FF72FA5ACF63F8FE" blockId="1.[151,1437,1078,1889]" pageId="1" pageNumber="382">
According to our data, the egg has an oval shape close to quadrangular with rounded corners (
<figureCitation id="87982A1EFFAB0E12FA90FA5AC9EAFA3E" box="[1317,1423,1415,1441]" captionStart="FIGURES 14" captionStartId="2.[151,264,1943,1967]" captionTargetBox="[246,1341,181,1919]" captionTargetId="figure-18@2.[246,1341,181,1919]" captionTargetPageId="2" captionText="FIGURES 14. Egg morphology of Ephemera (Ephemera) orientalis: 12, general shape; 34, micropyle of the complex extrachorion-adhesive layer. CM—canal micropylar; MO—micropylar opening; mp—micropyle; SG—sperm guide." figureDoi="http://doi.org/10.5281/zenodo.13618717" httpUri="https://zenodo.org/record/13618717/files/figure.png" pageId="1" pageNumber="382">Figs 12</figureCitation>
). Dimensions: 173.0206.0 µm in length (191.3 µm) and 104.1123.4 µm in width (111.0 µm). The attachment structure is a complex extrachorion-adhesive layer (AL-Ex) that covers the entire surface of the chorion (CS) (
<figureCitation id="87982A1EFFAB0E12FADFFA0CCCA6F98E" captionStart="FIGURES 14" captionStartId="2.[151,264,1943,1967]" captionTargetBox="[246,1341,181,1919]" captionTargetId="figure-18@2.[246,1341,181,1919]" captionTargetPageId="2" captionText="FIGURES 14. Egg morphology of Ephemera (Ephemera) orientalis: 12, general shape; 34, micropyle of the complex extrachorion-adhesive layer. CM—canal micropylar; MO—micropylar opening; mp—micropyle; SG—sperm guide." figureDoi="http://doi.org/10.5281/zenodo.13618717" httpUri="https://zenodo.org/record/13618717/files/figure.png" pageId="1" pageNumber="382">Figs 12</figureCitation>
,
<figureCitation id="87982A1EFFAB0E12FF65FA2BCC9BF98E" box="[208,254,1526,1553]" captionStart="FIGURES 58" captionStartId="3.[151,264,1943,1967]" captionTargetBox="[232,1355,181,1919]" captionTargetId="figure-16@3.[232,1355,181,1919]" captionTargetPageId="3" captionText="FIGURES 58. Egg morphology of Ephemera (Ephemera) orientalis: 56, egg surface without complex extrachorion-adhesive layer; 7, proximal part of micropylar canal on chorion surface; 8, thickness of the layer forming the eggshell. AL-Ex—complex adhesive-extrachorion layer; CS—chorion surface; MO—micropylar opening; mp—micropyle." figureDoi="http://doi.org/10.5281/zenodo.13618721" httpUri="https://zenodo.org/record/13618721/files/figure.png" pageId="1" pageNumber="382">56</figureCitation>
). Its thickness for
<taxonomicName id="D8A34D18FFAB0E12FE7BFA2ACE3CF98E" authorityName="McLachlan" authorityYear="1875" box="[462,601,1527,1553]" class="Insecta" family="Ephemeridae" genus="Ephemera" kingdom="Animalia" order="Ephemeroptera" pageId="1" pageNumber="382" phylum="Arthropoda" rank="species" species="orientalis">
<emphasis id="2DD7EA89FFAB0E12FE7BFA2ACE3CF98E" box="[462,601,1527,1553]" italics="true" pageId="1" pageNumber="382">E. orientalis</emphasis>
</taxonomicName>
is 1.6 1.8 µm (
<figureCitation id="87982A1EFFAB0E12FCA0FA2ACF3FF98E" box="[789,858,1527,1553]" captionStart="FIGURES 58" captionStartId="3.[151,264,1943,1967]" captionTargetBox="[232,1355,181,1919]" captionTargetId="figure-16@3.[232,1355,181,1919]" captionTargetPageId="3" captionText="FIGURES 58. Egg morphology of Ephemera (Ephemera) orientalis: 56, egg surface without complex extrachorion-adhesive layer; 7, proximal part of micropylar canal on chorion surface; 8, thickness of the layer forming the eggshell. AL-Ex—complex adhesive-extrachorion layer; CS—chorion surface; MO—micropylar opening; mp—micropyle." figureDoi="http://doi.org/10.5281/zenodo.13618721" httpUri="https://zenodo.org/record/13618721/files/figure.png" pageId="1" pageNumber="382">Fig. 8</figureCitation>
). The surface of the adhesive layer (AL) is slightly bumpy (
<figureCitation id="87982A1EFFAB0E12FF4DF9C1CD04F9A9" box="[248,353,1564,1590]" captionStart="FIGURES 14" captionStartId="2.[151,264,1943,1967]" captionTargetBox="[246,1341,181,1919]" captionTargetId="figure-18@2.[246,1341,181,1919]" captionTargetPageId="2" captionText="FIGURES 14. Egg morphology of Ephemera (Ephemera) orientalis: 12, general shape; 34, micropyle of the complex extrachorion-adhesive layer. CM—canal micropylar; MO—micropylar opening; mp—micropyle; SG—sperm guide." figureDoi="http://doi.org/10.5281/zenodo.13618717" httpUri="https://zenodo.org/record/13618717/files/figure.png" pageId="1" pageNumber="382">Figs 12</figureCitation>
). There are one or two micropyles (mp) per egg in the equatorial area (
<figureCitation id="87982A1EFFAB0E12FB06F9C1C979F9A9" box="[1203,1308,1564,1590]" captionStart="FIGURES 14" captionStartId="2.[151,264,1943,1967]" captionTargetBox="[246,1341,181,1919]" captionTargetId="figure-18@2.[246,1341,181,1919]" captionTargetPageId="2" captionText="FIGURES 14. Egg morphology of Ephemera (Ephemera) orientalis: 12, general shape; 34, micropyle of the complex extrachorion-adhesive layer. CM—canal micropylar; MO—micropylar opening; mp—micropyle; SG—sperm guide." figureDoi="http://doi.org/10.5281/zenodo.13618717" httpUri="https://zenodo.org/record/13618717/files/figure.png" pageId="1" pageNumber="382">Figs 12</figureCitation>
,
<figureCitation id="87982A1EFFAB0E12FA99F9C1C93FF9A8" box="[1324,1370,1564,1591]" captionStart="FIGURES 58" captionStartId="3.[151,264,1943,1967]" captionTargetBox="[232,1355,181,1919]" captionTargetId="figure-16@3.[232,1355,181,1919]" captionTargetPageId="3" captionText="FIGURES 58. Egg morphology of Ephemera (Ephemera) orientalis: 56, egg surface without complex extrachorion-adhesive layer; 7, proximal part of micropylar canal on chorion surface; 8, thickness of the layer forming the eggshell. AL-Ex—complex adhesive-extrachorion layer; CS—chorion surface; MO—micropylar opening; mp—micropyle." figureDoi="http://doi.org/10.5281/zenodo.13618721" httpUri="https://zenodo.org/record/13618721/files/figure.png" pageId="1" pageNumber="382">56</figureCitation>
). The micropyles are “tagenoform-type,” with a well-expressed sperm guide (SG) oval and relatively deep (
<figureCitation id="87982A1EFFAB0E12FA92F99CC9F5F9C4" box="[1319,1424,1601,1627]" captionStart="FIGURES 14" captionStartId="2.[151,264,1943,1967]" captionTargetBox="[246,1341,181,1919]" captionTargetId="figure-18@2.[246,1341,181,1919]" captionTargetPageId="2" captionText="FIGURES 14. Egg morphology of Ephemera (Ephemera) orientalis: 12, general shape; 34, micropyle of the complex extrachorion-adhesive layer. CM—canal micropylar; MO—micropylar opening; mp—micropyle; SG—sperm guide." figureDoi="http://doi.org/10.5281/zenodo.13618717" httpUri="https://zenodo.org/record/13618717/files/figure.png" pageId="1" pageNumber="382">Figs 34</figureCitation>
). Sperm guide is 5.810.4 µm long and 5.06.1 µm wide. It should be noted that the sperm guide is only clearly visible on the adhesive surface of the egg. Once the adhesive layer has been removed, the sperm guide is not visible on the chorion (
<figureCitation id="87982A1EFFAB0E12FEFCF96CCDCBF954" box="[329,430,1713,1739]" captionStart="FIGURES 14" captionStartId="2.[151,264,1943,1967]" captionTargetBox="[246,1341,181,1919]" captionTargetId="figure-18@2.[246,1341,181,1919]" captionTargetPageId="2" captionText="FIGURES 14. Egg morphology of Ephemera (Ephemera) orientalis: 12, general shape; 34, micropyle of the complex extrachorion-adhesive layer. CM—canal micropylar; MO—micropylar opening; mp—micropyle; SG—sperm guide." figureDoi="http://doi.org/10.5281/zenodo.13618717" httpUri="https://zenodo.org/record/13618717/files/figure.png" pageId="1" pageNumber="382">Figs 3, 4</figureCitation>
,
<figureCitation id="87982A1EFFAB0E12FE0FF96CCDAFF954" box="[442,458,1713,1739]" captionStart="FIGURES 58" captionStartId="3.[151,264,1943,1967]" captionTargetBox="[232,1355,181,1919]" captionTargetId="figure-16@3.[232,1355,181,1919]" captionTargetPageId="3" captionText="FIGURES 58. Egg morphology of Ephemera (Ephemera) orientalis: 56, egg surface without complex extrachorion-adhesive layer; 7, proximal part of micropylar canal on chorion surface; 8, thickness of the layer forming the eggshell. AL-Ex—complex adhesive-extrachorion layer; CS—chorion surface; MO—micropylar opening; mp—micropyle." figureDoi="http://doi.org/10.5281/zenodo.13618721" httpUri="https://zenodo.org/record/13618721/files/figure.png" pageId="1" pageNumber="382">6</figureCitation>
). The micropylar canal, 1218 µm long and 46 µm wide, protrudes slightly above the adhesive layer (
<figureCitation id="87982A1EFFAB0E12FEFFF90ACDD4F96E" box="[330,433,1751,1777]" captionStart="FIGURES 14" captionStartId="2.[151,264,1943,1967]" captionTargetBox="[246,1341,181,1919]" captionTargetId="figure-18@2.[246,1341,181,1919]" captionTargetPageId="2" captionText="FIGURES 14. Egg morphology of Ephemera (Ephemera) orientalis: 12, general shape; 34, micropyle of the complex extrachorion-adhesive layer. CM—canal micropylar; MO—micropylar opening; mp—micropyle; SG—sperm guide." figureDoi="http://doi.org/10.5281/zenodo.13618717" httpUri="https://zenodo.org/record/13618717/files/figure.png" pageId="1" pageNumber="382">Figs 23</figureCitation>
,
<figureCitation id="87982A1EFFAB0E12FE0BF90BCDABF96E" box="[446,462,1750,1777]" captionStart="FIGURES 58" captionStartId="3.[151,264,1943,1967]" captionTargetBox="[232,1355,181,1919]" captionTargetId="figure-16@3.[232,1355,181,1919]" captionTargetPageId="3" captionText="FIGURES 58. Egg morphology of Ephemera (Ephemera) orientalis: 56, egg surface without complex extrachorion-adhesive layer; 7, proximal part of micropylar canal on chorion surface; 8, thickness of the layer forming the eggshell. AL-Ex—complex adhesive-extrachorion layer; CS—chorion surface; MO—micropylar opening; mp—micropyle." figureDoi="http://doi.org/10.5281/zenodo.13618721" httpUri="https://zenodo.org/record/13618721/files/figure.png" pageId="1" pageNumber="382">5</figureCitation>
). As shown previously (
<bibRefCitation id="7B324B6AFFAB0E12FD56F90AC87EF96E" author="Ubero-Pascal, N. A. &amp; Puig, M. A." box="[739,1051,1751,1777]" pageId="1" pageNumber="382" pagination="15 - 29" refId="ref7527" refString="Ubero-Pascal, N. A. &amp; Puig, M. A. (2007) Egg morphology update based on new chorionic data of Potamanthus luteus (Linnaeus), Ephemera danica Muller and Oligoneuriella rhenana (Imhoff) (Insecta, Ephemeroptera) obtained by scanning electron microscopy. Zootaxa, 1465 (1), 15 - 29. https: // doi. org / 10.11646 / zootaxa. 1465.1.2" type="journal article" year="2007">Ubero-Pascal &amp; Puig 2007</bibRefCitation>
), features of the chorion structure are visible on SEM only after removal of the adhesive layer. For
<emphasis id="2DD7EA89FFAB0E12FCCEF920C868F889" box="[891,1037,1788,1814]" italics="true" pageId="1" pageNumber="382">
<taxonomicName id="D8A34D18FFAB0E12FCCEF920C86CF889" authorityName="McLachlan" authorityYear="1875" box="[891,1033,1788,1814]" class="Insecta" family="Ephemeridae" genus="Ephemera" kingdom="Animalia" order="Ephemeroptera" pageId="1" pageNumber="382" phylum="Arthropoda" rank="species" species="orientalis">E. orientalis</taxonomicName>
,
</emphasis>
the surface of the chorion is finely wrinkled (
<figureCitation id="87982A1EFFAB0E12FEBFF8FCCD17F8A3" box="[266,370,1825,1852]" captionStart="FIGURES 58" captionStartId="3.[151,264,1943,1967]" captionTargetBox="[232,1355,181,1919]" captionTargetId="figure-16@3.[232,1355,181,1919]" captionTargetPageId="3" captionText="FIGURES 58. Egg morphology of Ephemera (Ephemera) orientalis: 56, egg surface without complex extrachorion-adhesive layer; 7, proximal part of micropylar canal on chorion surface; 8, thickness of the layer forming the eggshell. AL-Ex—complex adhesive-extrachorion layer; CS—chorion surface; MO—micropylar opening; mp—micropyle." figureDoi="http://doi.org/10.5281/zenodo.13618721" httpUri="https://zenodo.org/record/13618721/files/figure.png" pageId="1" pageNumber="382">Figs 56</figureCitation>
). The thickened proximal part of the tunnel-type micropylar canal and micropylar opening are clearly visible on the surface of the chorion. (
<figureCitation id="87982A1EFFAB0E12FD26F89ACE9CF8FE" box="[659,761,1863,1889]" captionStart="FIGURES 58" captionStartId="3.[151,264,1943,1967]" captionTargetBox="[232,1355,181,1919]" captionTargetId="figure-16@3.[232,1355,181,1919]" captionTargetPageId="3" captionText="FIGURES 58. Egg morphology of Ephemera (Ephemera) orientalis: 56, egg surface without complex extrachorion-adhesive layer; 7, proximal part of micropylar canal on chorion surface; 8, thickness of the layer forming the eggshell. AL-Ex—complex adhesive-extrachorion layer; CS—chorion surface; MO—micropylar opening; mp—micropyle." figureDoi="http://doi.org/10.5281/zenodo.13618721" httpUri="https://zenodo.org/record/13618721/files/figure.png" pageId="1" pageNumber="382">Figs 67</figureCitation>
).
</paragraph>
</subSubSection>
</treatment>
</document>