<documentID-CLB-Dataset="147460"ID-DOI="http://dx.doi.org/10.3897/phytokeys.208.89913"ID-GBIF-Dataset="fc59b0b6-86fd-4578-b942-aca758227fd5"ID-PMC="PMC9848972"ID-Pensoft-Pub="1314-2003-208-103"ID-Pensoft-UUID="88C0802178725C0F86DB6E2074FDD5AB"ID-PubMed="36761401"ModsDocID="1314-2003-208-103"checkinTime="1663785939964"checkinUser="pensoft"docAuthor="Lobban, Christopher S., Ashworth, Matt P., Camacho, Terance, Lam, Daryl W. & Theriot, Edward C."docDate="2022"docId="BE45F726136F55E0BC895DA3396ABAE3"docLanguage="en"docName="PhytoKeys 208: 103-184"docOrigin="PhytoKeys 208"docPubDate="2022-09-21"docSource="http://dx.doi.org/10.3897/phytokeys.208.89913"docTitle="Ardissonea densistriata Lobban 2022, sp. nov."docType="treatment"docVersion="6"id="88C0802178725C0F86DB6E2074FDD5AB"lastPageNumber="103"masterDocId="88C0802178725C0F86DB6E2074FDD5AB"masterDocTitle="Revision of Ardissoneaceae (Bacillariophyta, Mediophyceae) from Micronesian populations, with descriptions of two new genera, Ardissoneopsis and Grunowago, and new species in Ardissonea, Synedrosphenia and Climacosphenia"masterLastPageNumber="184"masterPageNumber="103"pageNumber="103"updateTime="1732928098777"updateUser="ExternalLinkService">
<mods:titleid="88AACE4B8B1EE6DF6127F84935451CC6">Revision of Ardissoneaceae (Bacillariophyta, Mediophyceae) from Micronesian populations, with descriptions of two new genera, Ardissoneopsis and Grunowago, and new species in Ardissonea, Synedrosphenia and Climacosphenia</mods:title>
<mods:affiliationid="BB1C5C0846743182D9C9BD0BA2856862">Division of Natural Sciences, University of Guam, Mangilao, GU 96923, Guam, USA</mods:affiliation>
<mods:namePartid="6AD20EDD84C052CE2CA1CC02C4310D37">Ashworth, Matt P.</mods:namePart>
<mods:affiliationid="DF02D2C1A617F0B71C5B592B407D6616">Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas, USA</mods:affiliation>
<mods:affiliationid="452A4C30DDFA0E5E4A391DD210EFE5B9">Division of Natural Sciences, University of Guam, Mangilao, GU 96923, Guam, USA</mods:affiliation>
<mods:namePartid="A4BA1E73DC94293E9EB711FF9A69DA08">Theriot, Edward C.</mods:namePart>
<mods:affiliationid="3F9DA7FE26AA6C1AB17CFC4654AA610B">Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas, USA</mods:affiliation>
<figureCitationid="060DBBE4245C22CED273E31575BFC83C"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Ardissonea densistriata sp. nov. A holotype from Bikar Atoll, BA 1 (LM) B, C external views of valves in SEM; arrows on B point to location of annulus (B Y 42 - 1, C BA- 1) D, E external view of two ends of same frustule showing isopolarity; arrow on E points to apical notch in VC (J 5) F, G internal views of valves with one and two middle lines of foramina, arrows showing asymmetrical pseudoseptum on valve in F and asymmetrical pole of the valvocopula in G; arrowheads point to longitudinal costae (BA 1) H, I external views of frustules from Bikar and Jaluit, respectively, showing profile of pole of valvocopula in H and apical comb of fimbriae on copula in I (arrow). Scale bars: 10 µm (A-C); 5 µm (D, E); 2.5 µm (F-I)."figureDoi="10.3897/phytokeys.208.89913.figure2"httpUri="https://binary.pensoft.net/fig/746718"pageId="0"pageNumber="103">Fig. 2</figureCitation>
<figureCitationid="A3E3DE4251463CD1A1254AE3B990AC16"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Ardissonea densistriata sp. nov. A holotype from Bikar Atoll, BA 1 (LM) B, C external views of valves in SEM; arrows on B point to location of annulus (B Y 42 - 1, C BA- 1) D, E external view of two ends of same frustule showing isopolarity; arrow on E points to apical notch in VC (J 5) F, G internal views of valves with one and two middle lines of foramina, arrows showing asymmetrical pseudoseptum on valve in F and asymmetrical pole of the valvocopula in G; arrowheads point to longitudinal costae (BA 1) H, I external views of frustules from Bikar and Jaluit, respectively, showing profile of pole of valvocopula in H and apical comb of fimbriae on copula in I (arrow). Scale bars: 10 µm (A-C); 5 µm (D, E); 2.5 µm (F-I)."figureDoi="10.3897/phytokeys.208.89913.figure2"httpUri="https://binary.pensoft.net/fig/746718"pageId="0"pageNumber="103">2A-C</figureCitation>
<figureCitationid="9705D579203ED9AEC22C17BEB263AF9A"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Ardissonea densistriata sp. nov. A holotype from Bikar Atoll, BA 1 (LM) B, C external views of valves in SEM; arrows on B point to location of annulus (B Y 42 - 1, C BA- 1) D, E external view of two ends of same frustule showing isopolarity; arrow on E points to apical notch in VC (J 5) F, G internal views of valves with one and two middle lines of foramina, arrows showing asymmetrical pseudoseptum on valve in F and asymmetrical pole of the valvocopula in G; arrowheads point to longitudinal costae (BA 1) H, I external views of frustules from Bikar and Jaluit, respectively, showing profile of pole of valvocopula in H and apical comb of fimbriae on copula in I (arrow). Scale bars: 10 µm (A-C); 5 µm (D, E); 2.5 µm (F-I)."figureDoi="10.3897/phytokeys.208.89913.figure2"httpUri="https://binary.pensoft.net/fig/746718"pageId="0"pageNumber="103">2B, C</figureCitation>
<figureCitationid="65A5D3FE94905783B8CAC67851AD0AFB"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Ardissonea densistriata sp. nov. A holotype from Bikar Atoll, BA 1 (LM) B, C external views of valves in SEM; arrows on B point to location of annulus (B Y 42 - 1, C BA- 1) D, E external view of two ends of same frustule showing isopolarity; arrow on E points to apical notch in VC (J 5) F, G internal views of valves with one and two middle lines of foramina, arrows showing asymmetrical pseudoseptum on valve in F and asymmetrical pole of the valvocopula in G; arrowheads point to longitudinal costae (BA 1) H, I external views of frustules from Bikar and Jaluit, respectively, showing profile of pole of valvocopula in H and apical comb of fimbriae on copula in I (arrow). Scale bars: 10 µm (A-C); 5 µm (D, E); 2.5 µm (F-I)."figureDoi="10.3897/phytokeys.208.89913.figure2"httpUri="https://binary.pensoft.net/fig/746718"pageId="0"pageNumber="103">2A-E</figureCitation>
<figureCitationid="6A79421B21EB2D85A9DE7E24D29B54F9"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Ardissonea densistriata sp. nov. A holotype from Bikar Atoll, BA 1 (LM) B, C external views of valves in SEM; arrows on B point to location of annulus (B Y 42 - 1, C BA- 1) D, E external view of two ends of same frustule showing isopolarity; arrow on E points to apical notch in VC (J 5) F, G internal views of valves with one and two middle lines of foramina, arrows showing asymmetrical pseudoseptum on valve in F and asymmetrical pole of the valvocopula in G; arrowheads point to longitudinal costae (BA 1) H, I external views of frustules from Bikar and Jaluit, respectively, showing profile of pole of valvocopula in H and apical comb of fimbriae on copula in I (arrow). Scale bars: 10 µm (A-C); 5 µm (D, E); 2.5 µm (F-I)."figureDoi="10.3897/phytokeys.208.89913.figure2"httpUri="https://binary.pensoft.net/fig/746718"pageId="0"pageNumber="103">2F, G</figureCitation>
<figureCitationid="54F03C30D3AB5C905628C601EA7F50F2"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Ardissonea densistriata sp. nov. A holotype from Bikar Atoll, BA 1 (LM) B, C external views of valves in SEM; arrows on B point to location of annulus (B Y 42 - 1, C BA- 1) D, E external view of two ends of same frustule showing isopolarity; arrow on E points to apical notch in VC (J 5) F, G internal views of valves with one and two middle lines of foramina, arrows showing asymmetrical pseudoseptum on valve in F and asymmetrical pole of the valvocopula in G; arrowheads point to longitudinal costae (BA 1) H, I external views of frustules from Bikar and Jaluit, respectively, showing profile of pole of valvocopula in H and apical comb of fimbriae on copula in I (arrow). Scale bars: 10 µm (A-C); 5 µm (D, E); 2.5 µm (F-I)."figureDoi="10.3897/phytokeys.208.89913.figure2"httpUri="https://binary.pensoft.net/fig/746718"pageId="0"pageNumber="103">2F</figureCitation>
<figureCitationid="F217CE03921EC12D62A8D15218DD6F23"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Ardissonea densistriata sp. nov. A holotype from Bikar Atoll, BA 1 (LM) B, C external views of valves in SEM; arrows on B point to location of annulus (B Y 42 - 1, C BA- 1) D, E external view of two ends of same frustule showing isopolarity; arrow on E points to apical notch in VC (J 5) F, G internal views of valves with one and two middle lines of foramina, arrows showing asymmetrical pseudoseptum on valve in F and asymmetrical pole of the valvocopula in G; arrowheads point to longitudinal costae (BA 1) H, I external views of frustules from Bikar and Jaluit, respectively, showing profile of pole of valvocopula in H and apical comb of fimbriae on copula in I (arrow). Scale bars: 10 µm (A-C); 5 µm (D, E); 2.5 µm (F-I)."figureDoi="10.3897/phytokeys.208.89913.figure2"httpUri="https://binary.pensoft.net/fig/746718"pageId="0"pageNumber="103">2F</figureCitation>
<figureCitationid="7D4D7C9AE9743245A33EC1E31B752F81"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Ardissonea densistriata sp. nov. A holotype from Bikar Atoll, BA 1 (LM) B, C external views of valves in SEM; arrows on B point to location of annulus (B Y 42 - 1, C BA- 1) D, E external view of two ends of same frustule showing isopolarity; arrow on E points to apical notch in VC (J 5) F, G internal views of valves with one and two middle lines of foramina, arrows showing asymmetrical pseudoseptum on valve in F and asymmetrical pole of the valvocopula in G; arrowheads point to longitudinal costae (BA 1) H, I external views of frustules from Bikar and Jaluit, respectively, showing profile of pole of valvocopula in H and apical comb of fimbriae on copula in I (arrow). Scale bars: 10 µm (A-C); 5 µm (D, E); 2.5 µm (F-I)."figureDoi="10.3897/phytokeys.208.89913.figure2"httpUri="https://binary.pensoft.net/fig/746718"pageId="0"pageNumber="103">2G</figureCitation>
<figureCitationid="03B2B3B6C3A9F1AC6F977CE289C0E3E1"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Ardissonea densistriata sp. nov. A holotype from Bikar Atoll, BA 1 (LM) B, C external views of valves in SEM; arrows on B point to location of annulus (B Y 42 - 1, C BA- 1) D, E external view of two ends of same frustule showing isopolarity; arrow on E points to apical notch in VC (J 5) F, G internal views of valves with one and two middle lines of foramina, arrows showing asymmetrical pseudoseptum on valve in F and asymmetrical pole of the valvocopula in G; arrowheads point to longitudinal costae (BA 1) H, I external views of frustules from Bikar and Jaluit, respectively, showing profile of pole of valvocopula in H and apical comb of fimbriae on copula in I (arrow). Scale bars: 10 µm (A-C); 5 µm (D, E); 2.5 µm (F-I)."figureDoi="10.3897/phytokeys.208.89913.figure2"httpUri="https://binary.pensoft.net/fig/746718"pageId="0"pageNumber="103">2D, I</figureCitation>
<figureCitationid="117E8325ACD8BCCE3AAAB8FAE56F6B71"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Ardissonea densistriata sp. nov. A holotype from Bikar Atoll, BA 1 (LM) B, C external views of valves in SEM; arrows on B point to location of annulus (B Y 42 - 1, C BA- 1) D, E external view of two ends of same frustule showing isopolarity; arrow on E points to apical notch in VC (J 5) F, G internal views of valves with one and two middle lines of foramina, arrows showing asymmetrical pseudoseptum on valve in F and asymmetrical pole of the valvocopula in G; arrowheads point to longitudinal costae (BA 1) H, I external views of frustules from Bikar and Jaluit, respectively, showing profile of pole of valvocopula in H and apical comb of fimbriae on copula in I (arrow). Scale bars: 10 µm (A-C); 5 µm (D, E); 2.5 µm (F-I)."figureDoi="10.3897/phytokeys.208.89913.figure2"httpUri="https://binary.pensoft.net/fig/746718"pageId="0"pageNumber="103">2D, I</figureCitation>
) with 1-3 irregular rows of pores on the exterior, pars interior with a row of small pores partially exposed to exterior and a fimbriate inner edge, particularly well developed at poles (Fig.
<figureCitationid="D4B9978DE7C37D1D9D741FD7BFE306B4"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Ardissonea densistriata sp. nov. A holotype from Bikar Atoll, BA 1 (LM) B, C external views of valves in SEM; arrows on B point to location of annulus (B Y 42 - 1, C BA- 1) D, E external view of two ends of same frustule showing isopolarity; arrow on E points to apical notch in VC (J 5) F, G internal views of valves with one and two middle lines of foramina, arrows showing asymmetrical pseudoseptum on valve in F and asymmetrical pole of the valvocopula in G; arrowheads point to longitudinal costae (BA 1) H, I external views of frustules from Bikar and Jaluit, respectively, showing profile of pole of valvocopula in H and apical comb of fimbriae on copula in I (arrow). Scale bars: 10 µm (A-C); 5 µm (D, E); 2.5 µm (F-I)."figureDoi="10.3897/phytokeys.208.89913.figure2"httpUri="https://binary.pensoft.net/fig/746718"pageId="0"pageNumber="103">2I</figureCitation>
<figureCitationid="1D028166CE9B311AC5ADB87AEEAB5081"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Ardissonea densistriata sp. nov. A holotype from Bikar Atoll, BA 1 (LM) B, C external views of valves in SEM; arrows on B point to location of annulus (B Y 42 - 1, C BA- 1) D, E external view of two ends of same frustule showing isopolarity; arrow on E points to apical notch in VC (J 5) F, G internal views of valves with one and two middle lines of foramina, arrows showing asymmetrical pseudoseptum on valve in F and asymmetrical pole of the valvocopula in G; arrowheads point to longitudinal costae (BA 1) H, I external views of frustules from Bikar and Jaluit, respectively, showing profile of pole of valvocopula in H and apical comb of fimbriae on copula in I (arrow). Scale bars: 10 µm (A-C); 5 µm (D, E); 2.5 µm (F-I)."figureDoi="10.3897/phytokeys.208.89913.figure2"httpUri="https://binary.pensoft.net/fig/746718"pageId="0"pageNumber="103">2D, E</figureCitation>
<figureCitationid="87A901B9BB2D968593466B13A47FB33B"captionStart="Figure 2"captionStartId="F2"captionText="Figure 2. Ardissonea densistriata sp. nov. A holotype from Bikar Atoll, BA 1 (LM) B, C external views of valves in SEM; arrows on B point to location of annulus (B Y 42 - 1, C BA- 1) D, E external view of two ends of same frustule showing isopolarity; arrow on E points to apical notch in VC (J 5) F, G internal views of valves with one and two middle lines of foramina, arrows showing asymmetrical pseudoseptum on valve in F and asymmetrical pole of the valvocopula in G; arrowheads point to longitudinal costae (BA 1) H, I external views of frustules from Bikar and Jaluit, respectively, showing profile of pole of valvocopula in H and apical comb of fimbriae on copula in I (arrow). Scale bars: 10 µm (A-C); 5 µm (D, E); 2.5 µm (F-I)."figureDoi="10.3897/phytokeys.208.89913.figure2"httpUri="https://binary.pensoft.net/fig/746718"pageId="0"pageNumber="103">2A</figureCitation>
. Filamentous seaweed sample from shallow subtidal farmer fish territory in the lagoon, precise location not recorded. Collection number BA1, October 2019. Andrew McInnis leg.
species, this species is easily distinguished by the small size, lanceolate shape and relatively high stria density. It is more readily confused in our flora with
<taxonomicNameid="C6B8027011223EE4B755F32CF77E411D"authorityName="Lobban & Ashworth"authorityYear="2022"family="Ardissoneaceae"genus="Grunowago"kingdom="Plantae"lsidName="Grunowago pacifica"order="Ardissoneales"pageId="0"pageNumber="103"rank="species"species="pacifica">
sp. nov. (see below), but in LM the latter can be distinguished by the lack of an inner wall (indicated by absence of foramina) and presence of a longitudinal costa along the midline, in SEM by the extensive poration on the copula. To date this species has not been observed in Guam samples. A different lanceolate, double-walled
' observed by Sabir et al. (unpublished: http://www.protistcentral.org/Taxa/get/taxa_id/586376 and Ashworth, personal communication). This unnamed species is 83-125
<figureCitationid="0EC1BAE09F64F729CD98607DCEFCF14F"captionStart="Figure 33"captionStartId="F33"captionText="Figure 33. Phylogenetic trees resulting from three analytical methods of the 3 - gene DNA sequence dataset assembled for this manuscript. Only the Ardissoneaceae and their sister group in the molecular phylogeny, Lampriscus, is shown here-the complete trees can be found in Suppl. materials 5 - 7: Figs S 3 - S 5 A Maximum Likelihood phylogeny resolved by RAxML, where values at nodes are bootstrap support values B Maximum Likelihood phylogeny resolved by IQTREE, where values at nodes are bootstrap support values C Bayesian Inference phylogeny resolved by ExaBayes, where values at nodes are posterior probability values. Strain taxonomy (name followed by DNA voucher ID or strain ID) follows currently accepted classification, with proposed genera based on morphology shown to the right of strain ID."figureDoi="10.3897/phytokeys.208.89913.figure33"httpUri="https://binary.pensoft.net/fig/746749"pageId="0"pageNumber="103">33</figureCitation>
