<documentid="AAFCF2DCC22F075C62C2FB5741A674CF"ID-DOI="10.5852/ejt.2018.429"ID-GBIF-Dataset="33bd05ac-2eba-486a-8bd9-afbd5916298f"ID-ISSN="2118-9773"ID-Zenodo-Dep="1255274"IM.illustrations_approvedBy="guilherme"IM.materialsCitations_approvedBy="guilherme"IM.metadata_approvedBy="guilherme"IM.taxonomicNames_approvedBy="felipe"IM.treatments_approvedBy="guilherme"checkinTime="1527623702466"checkinUser="plazi"docAuthor="Santamaría, Sergi, Enghoff, Henrik & Reboleira, Ana Sofía P. S."docDate="2018"docId="03E287DE8A0AFFCA72D0FC4EEECDFBB8"docLanguage="en"docName="ejt-429_santamaria_enghoff_repoleira.pdf.imf"docOrigin="European Journal of Taxonomy 429"docStyle="DocumentStyle:EF2B578F1D15862ADE45B0C07C620911.14:EJT.2018-.journal_article.type1"docStyleId="EF2B578F1D15862ADE45B0C07C620911"docStyleName="EJT.2018-.journal_article.type1"docStyleVersion="14"docTitle="Troglomyces dioicus Santamaría & Enghoff & Reboleira 2018, sp. nov."docType="treatment"docVersion="11"lastPageNumber="13"masterDocId="FFDBFFA68A00FFC67368FFDAEC64FFE8"masterDocTitle="New species of Troglomyces and Diplopodomyces (Laboulbeniales, Ascomycota) from millipedes (Diplopoda)"masterLastPageNumber="20"masterPageNumber="1"pageNumber="11"updateTime="1736261088761"updateUser="guilherme">
<mods:titleid="EB6135C792E494C28B1372B025D985C6">New species of Troglomyces and Diplopodomyces (Laboulbeniales, Ascomycota) from millipedes (Diplopoda)</mods:title>
<taxonomicNameid="4C4B4D4B8A0AFFCC72D0FC4EE87EFC47"ID-CoL="598Z5"authority="Santam., Enghoff & Reboleira"authorityName="Santamaría & Enghoff & Reboleira"authorityYear="2018"box="[440,1050,916,943]"class="Laboulbeniomycetes"family="Laboulbeniaceae"genus="Troglomyces"higherTaxonomySource="GBIF"kingdom="Fungi"order="Laboulbeniales"pageId="10"pageNumber="11"phylum="Ascomycota"rank="species"species="dioicus"status="sp. nov.">
<figureCitationid="13702A4D8A0AFFCC719FFC05EF58FC11"box="[759,828,991,1017]"captionStart="Fig. 4"captionStartId="11.[189,232,1852,1878]"captionTargetBox="[189,1399,1002,1811]"captionTargetId="figure@11.[189,1399,1002,1811]"captionTargetPageId="11"captionText="Fig. 4. Troglomyces dioicus Santam., Enghoff & Reboleira sp. nov. A–B. Mature female thalli with labelling of cells and other elements. C. Mature male thallus with labelling of cells and other elements. D. Paired immature thalli where original spore apex are labelled (sx for female at left; arrow for male at right). Scale bars: 50 µm (A‒B, D), 25 µm (C). Photographs from: SS E617a (A), SS E617c (B, D) and C-F-92269 (C)."httpUri="https://zenodo.org/record/1255282/files/figure.png"pageId="10"pageNumber="11">Fig. 4</figureCitation>
<paragraphid="8BF436C88A0AFFCC73D5FB89EE20FB51"blockId="10.[189,1399,1107,1209]"pageId="10"pageNumber="11">First dioecious species in the genus, with female thalli characterized by a very short cell III and a septum II-III placed under the level of the perithecial base, whereas in the other species cell III is taller and the septum II-III is clearly higher up.</paragraph>
<paragraphid="8BF436C88A0AFFCC73D5FAC8EFF8FAC4"blockId="10.[189,924,1298,1324]"box="[189,924,1298,1324]"pageId="10"pageNumber="11">Self-defined by its species epithet, meaning “two households”.</paragraph>
<materialsCitationid="3B233C958A0AFFCC7382FA5DE844F9F8"ID-GBIF-Occurrence="1846714277"collectedFrom="on Nepalmatoiulus sp. (Julida)"collectingDate="2014-06-10"collectingDateMax="2014-06-18"collectingDateMin="2014-06-10"collectorName="Brunke & Schillhammer"country="Myanmar"elevation="1730"latitude="22.969168"location="7 km NW Mogok, above road Mogok-Bemardmyo, S. Panlin village, west slope of Mt. Taung Mae"longLatPrecision="21"longitude="96.453606"municipality="Division"pageId="10"pageNumber="11"specimenCode="C-F-92269"specimenCount="1"stateProvince="Mandalay"typeStatus="holotype">
<locationid="8E9460138A0AFFCC713BFA76EE49FA03"LSID="urn:lsid:plazi:treatment:03E287DE8A0AFFCA72D0FC4EEECDFBB8:8E9460138A0AFFCC713BFA76EE49FA03"country="Myanmar"latitude="22.969168"longLatPrecision="21"longitude="96.453606"municipality="Division"name="7 km NW Mogok, above road Mogok-Bemardmyo, S. Panlin village, west slope of Mt. Taung Mae"pageId="10"pageNumber="11"stateProvince="Mandalay">
<materialsCitationid="3B233C958A0AFFCC7382F9E0ED35F977"ID-GBIF-Occurrence="1846714274"collectedFrom="Nepalmatoiulus sp. (Julida"collectingDate="2014-06-10"collectingDateMax="2014-06-18"collectingDateMin="2014-06-10"collectionCode="BCB- Mycotheca"collectorName="Brunke & Schillhammer"country="Myanmar"elevation="1730"latitude="22.969168"location="MYANMAR"longLatPrecision="21"longitude="96.453606"municipality="Division"pageId="10"pageNumber="11"specimenCode="SS E617a, SS E617c, SS E617d, SS E617e"specimenCount="1"stateProvince="Mandalay"typeStatus="isotype">
<paragraphid="8BF436C88A0AFFCC73D5F922E80CF8FA"blockId="10.[189,1128,1784,1811]"box="[189,1128,1784,1811]"pageId="10"pageNumber="11">Dioecious. Both male and female thalli are hyaline except for the darkened feet.</paragraph>
<paragraphid="8BF436C88A0AFFCC73D5F8E6EEB9F803"blockId="10.[189,1399,1852,2027]"pageId="10"pageNumber="11">Male thalli. Consisting of six superposed cells. Basal cell (I) about 3 times as long as broad. Suprabasal cell (II) small and squared. Cell III 1.5 times as long as broad. Primary septum well distinguished. Fourth cell 1.5 times as long as broad. Fifth cell squared, very small, bearing the single, apical, bottleshaped antheridium. No spiny remains of spore apex because it is coincident with the apex of efferent tube, i.e., where the discharging pore is found.</paragraph>
<paragraphid="8BF436C88A0BFFCD73D5FED1ED16FE5B"blockId="11.[189,1399,267,435]"pageId="11"pageNumber="12">Female thalli. Basal cell (I) twice or more as long as broad, enlarged distally, slightly constricted at the base. Suprabasal cell (II) as long as broad, laterally adnate to cell VI, broadened at base where in contact with cell I, narrowed toward distal end. Cell III very narrow, 3 times as long as broad, laterally adnate to perithecium. Septum I-II horizontal. Septum II-III horizontal or slightly oblique, below the level of septum VI-VII.</paragraph>
Primary appendage unbranched, forming an angle of almost 90º with main axis of thallus, consisting of up to 4 cells, of which the lower is about 4 times as long as broad, the others gradually shorter and tapering distally. Spiny remains of spore apex (sx) difficult to see in mature thalli (
<figureCitationid="13702A4D8A0BFFCD77F6FDFAE89BFDD2"box="[1182,1279,544,570]"captionStart="Fig. 4"captionStartId="11.[189,232,1852,1878]"captionTargetBox="[189,1399,1002,1811]"captionTargetId="figure@11.[189,1399,1002,1811]"captionTargetPageId="11"captionText="Fig. 4. Troglomyces dioicus Santam., Enghoff & Reboleira sp. nov. A–B. Mature female thalli with labelling of cells and other elements. C. Mature male thallus with labelling of cells and other elements. D. Paired immature thalli where original spore apex are labelled (sx for female at left; arrow for male at right). Scale bars: 50 µm (A‒B, D), 25 µm (C). Photographs from: SS E617a (A), SS E617c (B, D) and C-F-92269 (C)."httpUri="https://zenodo.org/record/1255282/files/figure.png"pageId="11"pageNumber="12">Fig. 4A</figureCitation>
), but well visible in very young thalli (
<figureCitationid="13702A4D8A0BFFCD7161FD99EE0CFDB5"box="[521,616,579,605]"captionStart="Fig. 4"captionStartId="11.[189,232,1852,1878]"captionTargetBox="[189,1399,1002,1811]"captionTargetId="figure@11.[189,1399,1002,1811]"captionTargetPageId="11"captionText="Fig. 4. Troglomyces dioicus Santam., Enghoff & Reboleira sp. nov. A–B. Mature female thalli with labelling of cells and other elements. C. Mature male thallus with labelling of cells and other elements. D. Paired immature thalli where original spore apex are labelled (sx for female at left; arrow for male at right). Scale bars: 50 µm (A‒B, D), 25 µm (C). Photographs from: SS E617a (A), SS E617c (B, D) and C-F-92269 (C)."httpUri="https://zenodo.org/record/1255282/files/figure.png"pageId="11"pageNumber="12">Fig. 4D</figureCitation>
).
</paragraph>
<paragraphid="8BF436C88A0BFFCD73D5FD5FED34FCA7"blockId="11.[189,1399,645,847]"pageId="11"pageNumber="12">Perithecial stalk cell (VI) triangular, as long as broad, resting in the concave apico-ventral side of cell II. Secondary stalk cell (VII) wedge-shaped, resting on the outer (ventral) corner of cell VI. Perithecium fusiform, stout, with broad venter, maximum width near lower 1/4‒1/5th, gradually tapering upwards, more abruptly upwards from w2-w3 septa. The tip is asymmetric, slightly bent dorsally, forming first a neat constriction, followed by a bulge and ending with four small lips. Dorsal side rather straight, ventral side convex.</paragraph>
<taxonomicNameid="4C4B4D4B8A0BFFCD7271F8E6EFE1F8BE"authority="Santam., Enghoff & Reboleira"authorityName="Santamaría & Enghoff & Reboleira"authorityYear="2018"box="[281,901,1852,1879]"class="Laboulbeniomycetes"family="Laboulbeniaceae"genus="Troglomyces"higherTaxonomySource="GBIF"kingdom="Fungi"order="Laboulbeniales"pageId="11"pageNumber="12"phylum="Ascomycota"rank="species"species="dioicus"status="sp. nov.">
. Paired immature thalli where original spore apex are labelled (sx for female at left; arrow for male at right). Scale bars: 50 µm (A‒B, D), 25 µm (C). Photographs from: SS E617a (A), SS E617c (B, D) and C-F-92269 (C).
</paragraph>
</caption>
<paragraphid="8BF436C88A0CFFCA73D5FED1E8A7FE86"blockId="12.[189,1398,267,366]"pageId="12"pageNumber="13">Length of male thalli from foot to apex of antheridium 88–93 µm. Antheridium 21–26 × 5–6 µm. Length of female thalli from foot to apex of perithecium 159‒201 µm. Perithecium (including basal cells) 91‒117 µm. Primary appendage (from primary septum to apex, undamaged) 50‒70 µm.</paragraph>
<figureCitationid="13702A4D8A0CFFCA73D5FD95ED72FD82"box="[189,278,591,618]"captionStart="Fig. 4"captionStartId="11.[189,232,1852,1878]"captionTargetBox="[189,1399,1002,1811]"captionTargetId="figure@11.[189,1399,1002,1811]"captionTargetPageId="11"captionText="Fig. 4. Troglomyces dioicus Santam., Enghoff & Reboleira sp. nov. A–B. Mature female thalli with labelling of cells and other elements. C. Mature male thallus with labelling of cells and other elements. D. Paired immature thalli where original spore apex are labelled (sx for female at left; arrow for male at right). Scale bars: 50 µm (A‒B, D), 25 µm (C). Photographs from: SS E617a (A), SS E617c (B, D) and C-F-92269 (C)."httpUri="https://zenodo.org/record/1255282/files/figure.png"pageId="12"pageNumber="13">Fig. 4A</figureCitation>
we have found a pair of females without any trace of antheridia or paired males (often seen as remains of the darkened feet, e.g.,
<figureCitationid="13702A4D8A0CFFCA713BFDA9EECAFD66"box="[595,686,627,654]"captionStart="Fig. 4"captionStartId="11.[189,232,1852,1878]"captionTargetBox="[189,1399,1002,1811]"captionTargetId="figure@11.[189,1399,1002,1811]"captionTargetPageId="11"captionText="Fig. 4. Troglomyces dioicus Santam., Enghoff & Reboleira sp. nov. A–B. Mature female thalli with labelling of cells and other elements. C. Mature male thallus with labelling of cells and other elements. D. Paired immature thalli where original spore apex are labelled (sx for female at left; arrow for male at right). Scale bars: 50 µm (A‒B, D), 25 µm (C). Photographs from: SS E617a (A), SS E617c (B, D) and C-F-92269 (C)."httpUri="https://zenodo.org/record/1255282/files/figure.png"pageId="12"pageNumber="13">Fig. 4B</figureCitation>
The species seems very rare and the thalli were scarce on legs 2, 3 and several anterior post-gonopodal legs. Undamaged mature male thalli with spermatia present in the efferent neck have been seen only twice (
<figureCitationid="13702A4D8A0CFFCA7265FD24ED0EFCF0"box="[269,362,766,792]"captionStart="Fig. 4"captionStartId="11.[189,232,1852,1878]"captionTargetBox="[189,1399,1002,1811]"captionTargetId="figure@11.[189,1399,1002,1811]"captionTargetPageId="11"captionText="Fig. 4. Troglomyces dioicus Santam., Enghoff & Reboleira sp. nov. A–B. Mature female thalli with labelling of cells and other elements. C. Mature male thallus with labelling of cells and other elements. D. Paired immature thalli where original spore apex are labelled (sx for female at left; arrow for male at right). Scale bars: 50 µm (A‒B, D), 25 µm (C). Photographs from: SS E617a (A), SS E617c (B, D) and C-F-92269 (C)."httpUri="https://zenodo.org/record/1255282/files/figure.png"pageId="12"pageNumber="13">Fig. 4C</figureCitation>
); immature thalli are more common and help us to understand that the efferent neck and pore (through which spermatia discharge) coincide with the original acute ascospore apex (
<figureCitationid="13702A4D8A0CFFCA7679FCF8E916FCD4"box="[1297,1394,802,828]"captionStart="Fig. 4"captionStartId="11.[189,232,1852,1878]"captionTargetBox="[189,1399,1002,1811]"captionTargetId="figure@11.[189,1399,1002,1811]"captionTargetPageId="11"captionText="Fig. 4. Troglomyces dioicus Santam., Enghoff & Reboleira sp. nov. A–B. Mature female thalli with labelling of cells and other elements. C. Mature male thallus with labelling of cells and other elements. D. Paired immature thalli where original spore apex are labelled (sx for female at left; arrow for male at right). Scale bars: 50 µm (A‒B, D), 25 µm (C). Photographs from: SS E617a (A), SS E617c (B, D) and C-F-92269 (C)."httpUri="https://zenodo.org/record/1255282/files/figure.png"pageId="12"pageNumber="13">Fig. 4D</figureCitation>
although most species are monoecious. A good example is the genus
<taxonomicNameid="4C4B4D4B8A0CFFCA7161FC76EFF7FC2E"authority="Mont. & C. P. Robin"authorityName="Mont. & C.P.Robin"box="[521,915,940,967]"class="Insecta"family="Pseudococcidae"genus="Laboulbenia"higherTaxonomySource="GBIF"kingdom="Animalia"order="Hemiptera"pageId="12"pageNumber="13"phylum="Arthropoda"rank="genus">
