treatments-xml/data/03/9A/AF/039AAF577F56FFBDFCDB560AFA7968F3.xml

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<mods:title id="7A3CB352D8C3C063CB2EF585CC03BF39">Morphological and Molecular Identification of Isospora sepetibensis (Chromista: Miozoa: Eimeriidae) from a New Host, Trichothraupis melanops (Passeriformes: Thraupidae: Tachyphoninae) in South America</mods:title>
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<mods:namePart id="177DD32DD1725B4FB55D44D2B1330693">Genovez-Oliveira, Jhon Lennon</mods:namePart>
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<mods:namePart id="C92E72D9C6618CD98BC8D8DAB27B5BE5">Cardozo, Sergian V.</mods:namePart>
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<mods:namePart id="158094EDA8A014DF4CDD5346F9AE31E1">De Oliveira, Águida A.</mods:namePart>
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<mods:namePart id="B5A6DB8E138835DA0BDE8135FC93C8F4">De Lima, Viviane M.</mods:namePart>
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<mods:namePart id="1674A6030FE089C3A1187FE808B33048">Ferreira, Ildemar</mods:namePart>
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<mods:namePart id="2DFB5F43B40BEFF3FF2122787C1EEFC6">Berto, Bruno P.</mods:namePart>
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<paragraph id="8B8C1E417F56FFBFFCDB560AFA5068F7" blockId="1.[809,1456,1619,1682]" pageId="1" pageNumber="18">
<heading id="D0C4A92D7F56FFBFFCDB560AFA5068F7" bold="true" centered="true" fontSize="11" level="2" pageId="1" pageNumber="18" reason="4">
<taxonomicName id="4C3365C27F56FFBFFCDB560AFDF368F7" authority="Berto, Flausino, Luz, Ferreira, Lopes, 2008" authorityName="Berto, Flausino, Luz, Ferreira, Lopes" authorityYear="2008" family="Eimeriidae" genus="Isospora" kingdom="Chromista" order="Eucoccidiida" pageId="1" pageNumber="18" phylum="Miozoa" rank="species" species="sepetibensis">
<emphasis id="B947C2537F56FFBFFCDB560AFBFE6808" bold="true" box="[809,1456,1619,1646]" pageId="1" pageNumber="18">
<emphasis id="B947C2537F56FFBFFCDB560AFA666808" bold="true" box="[809,1064,1619,1646]" italics="true" pageId="1" pageNumber="18">Isospora sepetibensis</emphasis>
<bibRefCitation id="EFA263B07F56FFBFFBC2560AFBFE6808" author="Berto B. P. &amp; Flausino W. &amp; Luz H. R. &amp; Ferreira I. &amp; Lopes C. W. G." box="[1072,1456,1620,1646]" pageId="1" pageNumber="18" pagination="77 - 81" refId="ref3966" refString="Berto B. P., Flausino W., Luz H. R., Ferreira I., Lopes C. W. G. (2008) Three new coccidian parasites of Brazilian tanager (Ramphoce- lus bresilius dorsalis) from South America. Acta Protozool., 47: 77 - 81" type="journal article" year="2008">Berto, Flausino, Luz, Ferreira,</bibRefCitation>
</emphasis>
<emphasis id="B947C2537F56FFBFFCDB5626FA5068F7" bold="true" box="[809,1054,1655,1682]" pageId="1" pageNumber="18">
Lopes, 2008 (
<figureCitation id="130802C47F56FFBFFC3F5629FA5A68F7" box="[973,1044,1655,1681]" captionStart="Fig" captionStartId="2.[132,167,1137,1159]" captionTargetBox="[128,1459,223,1108]" captionTargetId="figure-5@2.[128,1459,223,1108]" captionTargetPageId="2" captionText="Fig. 1. Photomicrographs of sporulated oocysts of Isospora sepetibensis, a coccidium species recovered from the black-goggled tanager Trichothraupis melanops. Note the inner (il) and outer (ol) layer of the oocyst wall, nucleus (n), polar granule (pg), Stieda body (sb), sub- Stieda body (ssb), sporocyst residuum (sr), striations (str) and the refractile body (rb). Sheather’s sugar solution. Scale-bar: 10 µm." figureDoi="http://doi.org/10.5281/zenodo.8356934" httpUri="https://zenodo.org/record/8356934/files/figure.png" pageId="1" pageNumber="18">Fig. 1</figureCitation>
)
</emphasis>
</taxonomicName>
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</paragraph>
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<paragraph id="8B8C1E417F56FFBFFCBD56F4FDCB69FF" blockId="1.[809,1456,1706,1945]" pageId="1" pageNumber="18">
<emphasis id="B947C2537F56FFBFFCBD56F4FAB068A2" bold="true" box="[847,1278,1706,1732]" pageId="1" pageNumber="18">Description of sporulated oocyst:</emphasis>
Oocyst shape (n = 18) sub-spherical to elongate ovoidal; 22–29 × 19–22 (25.9 × 20.7) length/width (L/W) ratio 1.1–1.4 (1.26). Wall bi-layered, 1.1–2.0 (1.3) thick, outer layer smooth, 
<emphasis id="B947C2537F56FFBFFC7D5767FDED6934" box="[911,931,1849,1874]" italics="true" pageId="1" pageNumber="18">c.</emphasis>
2/3 of total thickness. Micropyle and oocyst residuum absent, but one or two polar granules are present.
</paragraph>
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<paragraph id="8B8C1E417F55FFBCFF76542FFB256AD9" blockId="2.[132,1463,1137,1216]" pageId="2" pageNumber="19">
<emphasis id="B947C2537F55FFBCFF76542FFE886AE1" bold="true" box="[132,198,1137,1159]" pageId="2" pageNumber="19">Fig. 1.</emphasis>
Photomicrographs of sporulated oocysts of 
<taxonomicName id="4C3365C27F55FFBCFD84542CFD086AE1" authorityName="Berto, Flausino, Luz, Ferreira, Lopes" authorityYear="2008" box="[630,838,1138,1159]" family="Eimeriidae" genus="Isospora" kingdom="Chromista" order="Eucoccidiida" pageId="2" pageNumber="19" phylum="Miozoa" rank="species" species="sepetibensis">
<emphasis id="B947C2537F55FFBCFD84542CFD086AE1" box="[630,838,1138,1159]" italics="true" pageId="2" pageNumber="19">Isospora sepetibensis</emphasis>
</taxonomicName>
, a coccidium species recovered from the black-goggled tanager 
<taxonomicName id="4C3365C27F55FFBCFF7654D0FF3B6AC5" baseAuthorityName="Vieillot" baseAuthorityYear="1818" box="[132,373,1166,1187]" class="Aves" family="Thraupidae" genus="Trichothraupis" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="19" phylum="Chordata" rank="species" species="melanops">
<emphasis id="B947C2537F55FFBCFF7654D0FF3B6AC5" box="[132,373,1166,1187]" italics="true" pageId="2" pageNumber="19">Trichothraupis melanops</emphasis>
</taxonomicName>
. Note the inner (il) and outer (ol) layer of the oocyst wall, nucleus (n), polar granule (pg), Stieda body (sb), sub- Stieda body (ssb), sporocyst residuum (sr), striations (str) and the refractile body (rb). Sheather’s sugar solution. Scale-bar: 10 µm.
</paragraph>
</caption>
<paragraph id="8B8C1E417F55FFBCFF585511FFA86965" blockId="2.[132,779,1359,1945]" pageId="2" pageNumber="19">
<emphasis id="B947C2537F55FFBCFF585511FCFC6B0F" bold="true" box="[170,690,1359,1385]" pageId="2" pageNumber="19">Description of sporocyst and sporozoites:</emphasis>
Sporocysts (n = 18), ellipsoidal, 15–18 × 9–11 (16.8 × 10.3); L/W ratio 1.5–1.8 (1.65). Stieda body present, knob-like, 1.1–1.3 (1.2) high, 2.0–2.2 (2.1) wide; sub-Stieda body present, large to trapezoidal or, occasionally, irregular, 1.2–2.3 (1.7) high, 2.7–3.5 (3.1) wide; para-Stieda body absent. Sporocyst residuum present, consisting of numerous small granules dispersed between the sporozoites or as a distinctly sub-spherical body that appear to be membrane-bounded, 5.5–6.7 (6.1). Sporozoite vermiform with one posterior refractile body, centrally located nucleus and striations.
</paragraph>
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<paragraph id="8B8C1E417F55FFBCFF585751FCD56929" blockId="2.[132,779,1359,1945]" pageId="2" pageNumber="19">
<emphasis id="B947C2537F55FFBCFF585751FEA0694F" bold="true" box="[170,238,1807,1833]" pageId="2" pageNumber="19">Host:</emphasis>
<taxonomicName id="4C3365C27F55FFBCFEF05751FD44694F" authority="(Vieillot, 1818)" baseAuthorityName="Vieillot" baseAuthorityYear="1818" box="[258,778,1807,1833]" class="Aves" family="Thraupidae" genus="Trichothraupis" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="19" phylum="Chordata" rank="species" species="melanops">
<emphasis id="B947C2537F55FFBCFEF05751FC7B694F" box="[258,565,1807,1833]" italics="true" pageId="2" pageNumber="19">Trichothraupis melanops</emphasis>
(Vieillot, 1818)
</taxonomicName>
(
<taxonomicName id="4C3365C27F55FFBCFF7E576AFF796928" box="[140,311,1844,1870]" class="Aves" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="19" phylum="Chordata" rank="order">Passeriformes</taxonomicName>
: 
<taxonomicName id="4C3365C27F55FFBCFEB0576AFF826928" box="[322,460,1844,1870]" class="Aves" family="Thraupidae" kingdom="Animalia" order="Passeriformes" pageId="2" pageNumber="19" phylum="Chordata" rank="family">Thraupidae</taxonomicName>
: Tachyphoninae).
</paragraph>
</subSubSection>
<subSubSection id="C3294DCA7F55FFBCFF585707FA9268C2" pageId="2" pageNumber="19" type="materials_examined">
<paragraph id="8B8C1E417F55FFBCFF585707FC0869FF" blockId="2.[132,779,1359,1945]" pageId="2" pageNumber="19">
<emphasis id="B947C2537F55FFBCFF585707FF546915" bold="true" box="[170,282,1881,1907]" pageId="2" pageNumber="19">Locality:</emphasis>
<materialsCitation id="3B5B141C7F55FFBCFED95707FC0F69FF" country="Brazil" latitude="-22.438055" location="Itatiaia National Park" longLatPrecision="1" longitude="-44.62583" pageId="2" pageNumber="19" specimenCount="1">
<location id="8EEC489A7F55FFBCFED95707FC776915" LSID="urn:lsid:plazi:treatment:039AAF577F56FFBDFCDB560AFA7968F3:8EEC489A7F55FFBCFED95707FC776915" box="[299,569,1881,1907]" country="Brazil" latitude="-22.438055" longLatPrecision="1" longitude="-44.62583" name="Itatiaia National Park" pageId="2" pageNumber="19">Itatiaia National Park</location>
(
<geoCoordinate id="EE0778867F55FFBCFDA15707FD4B6915" box="[595,773,1881,1907]" degrees="22" direction="south" minutes="26" orientation="latitude" pageId="2" pageNumber="19" precision="1" seconds="17.00" value="-22.438055">22°26’17.00”S</geoCoordinate>
, 
<geoCoordinate id="EE0778867F55FFBCFF765721FF0D69FF" box="[132,323,1919,1945]" degrees="44" direction="west" minutes="37" orientation="longitude" pageId="2" pageNumber="19" precision="1" seconds="33.00" value="-44.62583">44°37’33.00”W</geoCoordinate>
), Southeastern 
<collectingCountry id="F3245ED17F55FFBCFE045721FC0F69FF" box="[502,577,1919,1945]" name="Brazil" pageId="2" pageNumber="19">Brazil</collectingCountry>
</materialsCitation>
.
</paragraph>
<paragraph id="8B8C1E417F55FFBCFCA45511FA9268C2" blockId="2.[816,1463,1359,1946]" pageId="2" pageNumber="19">
<emphasis id="B947C2537F55FFBCFCA45511FDAA6B0F" bold="true" box="[854,996,1359,1385]" pageId="2" pageNumber="19">Specimens:</emphasis>
Photomicrographs and oocysts in 2.5% K 
<subScript id="17B71C047F55FFBCFCB455DBFD016BF5" attach="right" box="[838,847,1413,1427]" fontSize="6" pageId="2" pageNumber="19">2</subScript>
Cr 
<subScript id="17B71C047F55FFBCFC9955DBFD3A6BF5" attach="both" box="[875,884,1413,1427]" fontSize="6" pageId="2" pageNumber="19">2</subScript>
O 
<subScript id="17B71C047F55FFBCFC7B55DBFDDC6BF5" attach="left" box="[905,914,1413,1427]" fontSize="6" pageId="2" pageNumber="19">7</subScript>
solution (
<bibRefCitation id="EFA263B07F55FFBCFBF6552CFABC6BEA" author="Williams R. B. &amp; Thebo P. &amp; Marshall R. N. &amp; Marshall J. A." box="[1028,1266,1394,1420]" pageId="2" pageNumber="19" pagination="69 - 76" refId="ref5377" refString="Williams R. B., Thebo P., Marshall R. N., Marshall J. A. (2010) Coc- cidian oocysts as type-specimens: long-term storage in aqueous potassium dichromate solution preserves DNA. Syst. Parasitol., 76: 69 - 76" type="journal article" year="2010">
Williams 
<emphasis id="B947C2537F55FFBCFB85552DFAFE6BEA" box="[1143,1200,1394,1420]" italics="true" pageId="2" pageNumber="19">et al.</emphasis>
2010
</bibRefCitation>
) are deposited at the Museu de Zoologia at the Universidade Federal Ru- ral do 
<collectingRegion id="49F7D0A37F55FFBCFC7255E6FA7F6BB4" box="[896,1073,1464,1490]" country="Brazil" name="Rio de Janeiro" pageId="2" pageNumber="19">Rio de Janeiro</collectingRegion>
, 
<collectingCountry id="F3245ED17F55FFBCFBCC55E6FAC76BB4" box="[1086,1161,1464,1490]" name="Brazil" pageId="2" pageNumber="19">Brazil</collectingCountry>
, under accession number MZURPTZ2019014. Photomicrographs are also de- posited and available (http://r1.ufrrj.br/labicoc/colecao. html) in the Parasitology Collection of the Laboratório de Biologia de Coccídios, at UFRRJ, under repository number 92/2019. Photographs of the host specimens are deposited in the same collection.
</paragraph>
</subSubSection>
<subSubSection id="C3294DCA7F55FFBCFCA456F3FB406935" pageId="2" pageNumber="19" type="biology_ecology">
<paragraph id="8B8C1E417F55FFBCFCA456F3FA2268A1" blockId="2.[816,1463,1359,1946]" box="[854,1132,1709,1735]" pageId="2" pageNumber="19">
<emphasis id="B947C2537F55FFBCFCA456F3FDA468A1" bold="true" box="[854,1002,1709,1735]" pageId="2" pageNumber="19">Site in host:</emphasis>
Unknown.
</paragraph>
<paragraph id="8B8C1E417F55FFBCFCA4568EFB30688D" blockId="2.[816,1463,1359,1946]" box="[854,1406,1744,1771]" pageId="2" pageNumber="19">
<emphasis id="B947C2537F55FFBCFCA4568EFDA6688C" bold="true" box="[854,1000,1744,1770]" pageId="2" pageNumber="19">Prevalence:</emphasis>
67% (15 out of 10 birds infected).
</paragraph>
<paragraph id="8B8C1E417F55FFBCFCA456ADFB406935" blockId="2.[816,1463,1359,1946]" pageId="2" pageNumber="19">
<emphasis id="B947C2537F55FFBCFCA456ADFABD696B" bold="true" box="[854,1267,1779,1805]" pageId="2" pageNumber="19">Representative DNA sequence:</emphasis>
Representative 
<emphasis id="B947C2537F55FFBCFCC25748FD266956" box="[816,872,1814,1840]" italics="true" pageId="2" pageNumber="19">cox1</emphasis>
sequence is deposited in the GenBank database under the accession number MK682606.
</paragraph>
</subSubSection>
<subSubSection id="C3294DCA7F55FFBDFCA45702FA7968F3" lastPageId="3" lastPageNumber="20" pageId="2" pageNumber="19" type="discussion">
<paragraph id="8B8C1E417F55FFBDFCA45702FA7968F3" blockId="2.[816,1463,1359,1946]" lastBlockId="3.[809,1456,1446,1685]" lastPageId="3" lastPageNumber="20" pageId="2" pageNumber="19">
<emphasis id="B947C2537F55FFBCFCA45702FA3D6910" bold="true" box="[854,1139,1884,1910]" pageId="2" pageNumber="19">Phylogenetic analysis:</emphasis>
The amplification of the DNA from the fifteen oocysts of 
<taxonomicName id="4C3365C27F55FFBCFB4957DEFB2D69FF" authorityName="Berto, Flausino, Luz, Ferreira, Lopes" authorityYear="2008" box="[1211,1379,1919,1945]" family="Eimeriidae" genus="Isospora" kingdom="Chromista" order="Eucoccidiida" pageId="2" pageNumber="19" phylum="Miozoa" rank="species" species="sepetibensis">
<emphasis id="B947C2537F55FFBCFB4957DEFB2D69FF" box="[1211,1379,1919,1945]" italics="true" pageId="2" pageNumber="19">I. sepetibensis</emphasis>
</taxonomicName>
recovered from 
<taxonomicName id="4C3365C27F54FFBDFF0E55F9FFC26BA6" baseAuthorityName="Vieillot" baseAuthorityYear="1818" box="[252,396,1446,1472]" class="Aves" family="Thraupidae" genus="Trichothraupis" kingdom="Animalia" order="Passeriformes" pageId="3" pageNumber="20" phylum="Chordata" rank="species" species="melanops">
<emphasis id="B947C2537F54FFBDFF0E55F9FFC26BA6" box="[252,396,1446,1472]" italics="true" pageId="3" pageNumber="20">T. melanops</emphasis>
</taxonomicName>
showed a clear band of 
<emphasis id="B947C2537F54FFBDFD4B55F9FC836BA6" box="[697,717,1447,1472]" italics="true" pageId="3" pageNumber="20">c.</emphasis>
250 bp. The phylogenetic analysis was constructed with 33 sequences of 
<taxonomicName id="4C3365C27F54FFBDFED455AEFF84686F" box="[294,458,1519,1545]" family="Eimeriidae" genus="Isospora" kingdom="Chromista" order="Eucoccidiida" pageId="3" pageNumber="20" phylum="Miozoa" rank="species" species="undetermined">
<emphasis id="B947C2537F54FFBDFED455AEFFC3686F" box="[294,397,1520,1545]" italics="true" pageId="3" pageNumber="20">Isospora</emphasis>
spp.
</taxonomicName>
closest to 
<taxonomicName id="4C3365C27F54FFBDFDA555AEFD4D686F" authorityName="Berto, Flausino, Luz, Ferreira, Lopes" authorityYear="2008" box="[599,771,1519,1545]" family="Eimeriidae" genus="Isospora" kingdom="Chromista" order="Eucoccidiida" pageId="3" pageNumber="20" phylum="Miozoa" rank="species" species="sepetibensis">
<emphasis id="B947C2537F54FFBDFDA555AEFD4D686F" box="[599,771,1519,1545]" italics="true" pageId="3" pageNumber="20">I. sepetibensis</emphasis>
</taxonomicName>
available on GenBank (
<figureCitation id="130802C47F54FFBDFE6B564DFFAA684B" box="[409,484,1555,1581]" captionStart="Fig" captionStartId="3.[125,160,1276,1298]" captionTargetBox="[142,1449,242,1246]" captionTargetId="figure-7@3.[125,1456,223,1263]" captionTargetPageId="3" captionText="Fig. 2. Maximum likelihood tree estimated from the cox1 sequences. Numbers at nodes represent bootstrap support (1,000 replicates; only values&gt; 50% shown) for Neighbor-Joining and Maximum Likelihood, respectively. The scale-bar represents the number of nucleotide substitutions per site." figureDoi="http://doi.org/10.5281/zenodo.8356936" httpUri="https://zenodo.org/record/8356936/files/figure.png" pageId="3" pageNumber="20">Fig. 2</figureCitation>
). 
<taxonomicName id="4C3365C27F54FFBDFE0E564AFF036834" authority="(Railliet, Lucet, 1891)" baseAuthorityName="Railliet, Lucet" baseAuthorityYear="1891" family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="3" pageNumber="20" phylum="Miozoa" rank="species" species="tenella">
<emphasis id="B947C2537F54FFBDFE0E564AFCFB684B" box="[508,693,1555,1581]" italics="true" pageId="3" pageNumber="20">Eimeria tenella</emphasis>
(Railliet, Lucet, 1891)
</taxonomicName>
was used as the outgroup. 
<taxonomicName id="4C3365C27F54FFBDFD6E5666FF456810" authorityName="Berto, Flausino, Luz, Ferreira, Lopes" authorityYear="2008" family="Eimeriidae" genus="Isospora" kingdom="Chromista" order="Eucoccidiida" pageId="3" pageNumber="20" phylum="Miozoa" rank="species" species="sepetibensis">
<emphasis id="B947C2537F54FFBDFD6E5666FF456810" italics="true" pageId="3" pageNumber="20">Isospora sepetibensis</emphasis>
</taxonomicName>
sat in a large group with the highest similarity of 98.1% with an 
<taxonomicName id="4C3365C27F54FFBDFE6F56DFFC6068FD" box="[413,558,1665,1691]" family="Eimeriidae" genus="Isospora" kingdom="Chromista" order="Eucoccidiida" pageId="3" pageNumber="20" phylum="Miozoa" rank="species" species="undetermined">
<emphasis id="B947C2537F54FFBDFE6F56DFFC4A68FC" box="[413,516,1665,1690]" italics="true" pageId="3" pageNumber="20">Isospora</emphasis>
sp.
</taxonomicName>
reported as pseudoparasite of bank voles 
<taxonomicName id="4C3365C27F54FFBDFE5F56FBFE8F6885" authority="(Schreber, 1780)" baseAuthorityName="Schreber" baseAuthorityYear="1780" class="Mammalia" family="Cricetidae" genus="Myodes" kingdom="Animalia" order="Rodentia" pageId="3" pageNumber="20" phylum="Chordata" rank="species" species="glareolus">
<emphasis id="B947C2537F54FFBDFE5F56FBFCCF68D9" box="[429,641,1701,1727]" italics="true" pageId="3" pageNumber="20">Myodes glareolus</emphasis>
(Schreber, 1780)
</taxonomicName>
in 
<collectingCountry id="F3245ED17F54FFBDFF105697FFD46885" box="[226,410,1737,1763]" name="Czech Republic" pageId="3" pageNumber="20">Czech Republic</collectingCountry>
(
<bibRefCitation id="EFA263B07F54FFBDFE545697FCE06885" author="Trefancova A. &amp; Macova A. &amp; Kvicerova J." box="[422,686,1737,1763]" pageId="3" pageNumber="20" pagination="104 - 120" refId="ref5287" refString="Trefancova A., Macova A., Kvicerova J. (2019) Isosporan Oocysts in the Faeces of Bank Voles (Myodes glareolus; Arvicolinae, Rodentia): Real Parasites, or Pseudoparasites?. Protist, 170: 104 - 120" type="journal article" year="2019">
Trefancová 
<emphasis id="B947C2537F54FFBDFDC35694FC246885" box="[561,618,1737,1763]" italics="true" pageId="3" pageNumber="20">et al.</emphasis>
2019
</bibRefCitation>
). It was close to the clade with 
<taxonomicName id="4C3365C27F54FFBDFE7956B0FFBC6961" authorityName="Schneider" authorityYear="1881" box="[395,498,1774,1799]" family="Eimeriidae" genus="Isospora" kingdom="Chromista" order="Eucoccidiida" pageId="3" pageNumber="20" phylum="Miozoa" rank="genus">
<emphasis id="B947C2537F54FFBDFE7956B0FFBC6961" box="[395,498,1774,1799]" italics="true" pageId="3" pageNumber="20">Isospora</emphasis>
</taxonomicName>
isolates from the spectacled warbler 
<taxonomicName id="4C3365C27F54FFBDFEDF574CFCAA694A" authority="Temminck, 1820" authorityName="Temminck" authorityYear="1820" box="[301,740,1810,1836]" class="Aves" family="Sylviidae" genus="Sylvia" kingdom="Animalia" order="Passeriformes" pageId="3" pageNumber="20" phylum="Chordata" rank="species" species="conspicillata">
<emphasis id="B947C2537F54FFBDFEDF574CFC5B694A" box="[301,533,1810,1836]" italics="true" pageId="3" pageNumber="20">Sylvia conspicillata</emphasis>
Temminck, 
<quantity id="4CCBB3A47F54FFBDFD5B574CFCAA694A" box="[681,740,1810,1836]" metricMagnitude="1" metricUnit="m" metricValue="4.6228" pageId="3" pageNumber="20" unit="in" value="1820.0">1820</quantity>
</taxonomicName>
in Macaronesia (
<bibRefCitation id="EFA263B07F54FFBDFED15768FFA16937" author="Illera J. C. &amp; Fernandez-Alvarez A. &amp; Hernandez-Flores C. N." box="[291,495,1846,1873]" pageId="3" pageNumber="20" pagination="1858 - 1870" refId="ref4557" refString="Illera J. C., Fernandez-Alvarez A., Hernandez-Flores C. N., Foron- da P. (2015) Unforeseen biogeographical patterns in a multiple parasite system in Macaronesia. J. Biogeogr., 42: 1858 - 1870" type="journal article" year="2015">
Illera 
<emphasis id="B947C2537F54FFBDFE9E5769FFE66936" box="[364,424,1846,1872]" italics="true" pageId="3" pageNumber="20">et al.</emphasis>
2015
</bibRefCitation>
); and also was close to the clade with 
<taxonomicName id="4C3365C27F54FFBDFEC35705FF9D6913" box="[305,467,1883,1909]" family="Eimeriidae" genus="Isospora" kingdom="Chromista" order="Eucoccidiida" pageId="3" pageNumber="20" phylum="Miozoa" rank="species" species="undetermined">
<emphasis id="B947C2537F54FFBDFEC35705FFD66912" box="[305,408,1883,1908]" italics="true" pageId="3" pageNumber="20">Isospora</emphasis>
spp.
</taxonomicName>
recently sequenced from Neotropical passerines (
<bibRefCitation id="EFA263B07F54FFBDFE515721FCB169FF" author="Silva-Carvalho L. M. &amp; Pastura D. G. N. &amp; Gomes J. V. &amp; Siqueira P. B. &amp; Rodrigues M. B. &amp; Lima V. M. &amp; Berto B. P." box="[419,767,1919,1945]" pageId="3" pageNumber="20" pagination="455 - 463" refId="ref5021" refString="Silva-Carvalho L. M., Pastura D. G. N., Gomes J. V., Siqueira P. B., Rodrigues M. B., Lima V. M., Berto B. P. (2018 a). Isospora lopesi n. sp. (Protozoa: Apicomplexa: Eimeriidae) from the eastern white-throated spadebill Platyrinchus mystaceus Vieillot (Passeriformes: Tyranni: Tyrannidae) in South America. Syst. Parasitol., 95: 455 - 463" type="journal article" year="2018">
Silva-Carvalho 
<emphasis id="B947C2537F54FFBDFD9757DEFCE869FF" box="[613,678,1919,1945]" italics="true" pageId="3" pageNumber="20">et al.</emphasis>
2018a
</bibRefCitation>
, 
<bibRefCitation id="EFA263B07F54FFBDFCDB55F8FD386BA6" author="Silva-Carvalho L. M. &amp; Pastura D. G. N. &amp; Rodrigues M. B. &amp; Gomes J. V. &amp; Oliveira M. S. &amp; Siqueira P. B. &amp; Oliveira J. L. G. &amp; Soares S. S. &amp; Oliveira A. A. &amp; Lima V. M. &amp; Ferreira I. &amp; Berto B. P." box="[809,886,1446,1472]" pageId="3" pageNumber="20" pagination="2635 - 2641" refId="ref5111" refString="Silva-Carvalho L. M., Pastura D. G. N., Rodrigues M. B., Gomes J. V., Oliveira M. S., Siqueira P. B., Oliveira J. L. G., Soares S. S., Oliveira A. A., Lima V. M., Ferreira I., Berto B. P. (2018 b). Isospora sagittulae McQuistion &amp; Capparella (1992 (Apicom- plexa: Eimeriidae) from antbirds (Passeriformes: Thamnophili- dae) in the Amazon and Atlantic Forest of Brazil: with notes on its distribution and dispersion in the Neotropical region. Parasi- tol. Res., 117: 2635 - 2641" type="journal article" year="2018">2018b</bibRefCitation>
; 
<bibRefCitation id="EFA263B07F54FFBDFC7655F8FAC36BA6" author="Rodrigues M. B. &amp; Oliveira J. L. G. &amp; Silva-Carvalho L. M. &amp; Pastura D. G. N. &amp; Gomes J. V. &amp; Oliveira M. S. &amp; Siqueira P. B. &amp; Oliveira A. A. &amp; Lima V. M. &amp; Ferreira I. &amp; Berto B. P." box="[900,1165,1446,1472]" pageId="3" pageNumber="20" refId="ref4751" refString="Rodrigues M. B., Oliveira J. L. G., Silva-Carvalho L. M., Pastura D. G. N., Gomes J. V., Oliveira M. S., Siqueira P. B., Oliveira A. A., Lima V. M., Ferreira I., Berto B. P. (2019). The vulner- able Sporophila frontalis (Verreaux) and Haplospiza unicolor Cabanis as new hosts for Isospora sporophilae Carvalho-Filho, Meireles, Ribeiro &amp; Lopes, 2005 (Eimeriidae) in Brazil. Syst. Parasitol. [in press]" type="book" year="2019">
Rodrigues 
<emphasis id="B947C2537F54FFBDFBF455F9FA0D6BA6" box="[1030,1091,1446,1472]" italics="true" pageId="3" pageNumber="20">et al.</emphasis>
2019
</bibRefCitation>
). Subsequently, a subset with only 215 bp long 
<emphasis id="B947C2537F54FFBDFBB25594FA396B82" box="[1088,1143,1482,1508]" italics="true" pageId="3" pageNumber="20">cox1</emphasis>
gene sequences was constructed (
<figureCitation id="130802C47F54FFBDFC6455B3FD936861" box="[918,989,1517,1543]" captionStart="Fig" captionStartId="4.[132,167,798,820]" captionTargetBox="[140,1450,251,766]" captionTargetId="figure-5@4.[132,1463,223,787]" captionTargetPageId="4" captionText="Fig. 3. Maximum likelihood tree estimated from the 215 bp long cox1 sequences. Numbers at nodes represent bootstrap support (1,000 replicates; only values&gt; 50% shown) for Neighbor-Joining and Maximum Likelihood, respectively. The scale-bar represents the number of nucleotide substitutions per site." figureDoi="http://doi.org/10.5281/zenodo.8356938" httpUri="https://zenodo.org/record/8356938/files/figure.png" pageId="3" pageNumber="20">Fig. 3</figureCitation>
). In this analysis, 
<taxonomicName id="4C3365C27F54FFBDFB4155B0FBE16861" authorityName="Berto, Flausino, Luz, Ferreira, Lopes" authorityYear="2008" box="[1203,1455,1517,1543]" family="Eimeriidae" genus="Isospora" kingdom="Chromista" order="Eucoccidiida" pageId="3" pageNumber="20" phylum="Miozoa" rank="species" species="sepetibensis">
<emphasis id="B947C2537F54FFBDFB4155B0FBE16861" box="[1203,1455,1517,1543]" italics="true" pageId="3" pageNumber="20">Isospora sepetibensis</emphasis>
</taxonomicName>
sat in the same clade of 
<taxonomicName id="4C3365C27F54FFBDFBB2564FFA90684D" box="[1088,1246,1553,1579]" family="Eimeriidae" genus="Isospora" kingdom="Chromista" order="Eucoccidiida" pageId="3" pageNumber="20" phylum="Miozoa" rank="species" species="undetermined">
<emphasis id="B947C2537F54FFBDFBB2564FFAE9684C" box="[1088,1191,1553,1578]" italics="true" pageId="3" pageNumber="20">Isospora</emphasis>
spp.
</taxonomicName>
from Neotropical passerines, with the highest similarity of 98.5% with 
<taxonomicName id="4C3365C27F54FFBDFCDB5606FB1B6814" authority="Silva-Carvalho &amp; Berto, 2018" authorityName="Silva-Carvalho &amp; Berto" authorityYear="2018" box="[809,1365,1624,1650]" family="Eimeriidae" genus="Isospora" kingdom="Chromista" order="Eucoccidiida" pageId="3" pageNumber="20" phylum="Miozoa" rank="species" species="lopesi">
<emphasis id="B947C2537F54FFBDFCDB5606FDAE6814" box="[809,992,1624,1650]" italics="true" pageId="3" pageNumber="20">Isospora lopesi</emphasis>
Silva-Carvalho &amp; Berto, 2018
</taxonomicName>
(
<bibRefCitation id="EFA263B07F54FFBDFA9A5606FA6468F3" author="Silva-Carvalho L. M. &amp; Pastura D. G. N. &amp; Gomes J. V. &amp; Siqueira P. B. &amp; Rodrigues M. B. &amp; Lima V. M. &amp; Berto B. P." pageId="3" pageNumber="20" pagination="455 - 463" refId="ref5021" refString="Silva-Carvalho L. M., Pastura D. G. N., Gomes J. V., Siqueira P. B., Rodrigues M. B., Lima V. M., Berto B. P. (2018 a). Isospora lopesi n. sp. (Protozoa: Apicomplexa: Eimeriidae) from the eastern white-throated spadebill Platyrinchus mystaceus Vieillot (Passeriformes: Tyranni: Tyrannidae) in South America. Syst. Parasitol., 95: 455 - 463" type="journal article" year="2018">
Silva-Carvalho 
<emphasis id="B947C2537F54FFBDFC6E5622FD9868F3" box="[924,982,1659,1685]" italics="true" pageId="3" pageNumber="20">et al.</emphasis>
2018a
</bibRefCitation>
).
</paragraph>
</subSubSection>
</treatment>
</document>