743 lines
132 KiB
XML
743 lines
132 KiB
XML
<document id="DE5F2D32AF17A805689D4C6094BD759E" ID-CLB-Dataset="31251" ID-DOI="10.11646/zootaxa.4362.1.5" ID-GBIF-Dataset="99c9eb85-21bc-470b-beda-e3f5fd023404" ID-ISSN="1175-5326" ID-Zenodo-Dep="1076306" ID-ZooBank="F72F750D-563E-4960-BAB3-CBEE139A288C" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1512393337967" checkinUser="plazi" docAuthor="Negrete, Lisandro & Brusa, Francisco" docDate="2017" docId="0397BB6CFFF9FFCDBDBE92CAC8E5FC08" docLanguage="en" docName="zootaxa.4362.1.5.pdf" docOrigin="Zootaxa 4362 (1)" docStyle="DocumentStyle:647186512141C8FC8976D5BCC54AEB7D.9:Zootaxa.2013-.journal_article" docStyleId="647186512141C8FC8976D5BCC54AEB7D" docStyleName="Zootaxa.2013-.journal_article" docStyleVersion="9" docTitle="Imbira negrita Negrete & Brusa 2017, sp. nov." docType="treatment" docVersion="8" lastPageNumber="112" masterDocId="FFAEC314FFFEFFC0BD299334CD6EFF88" masterDocTitle="Increasing diversity of land planarians (Platyhelminthes: Geoplanidae) in the Interior Atlantic Forest with the description of two new species and new records from Argentina" masterLastPageNumber="127" masterPageNumber="99" pageNumber="106" updateTime="1698506383038" updateUser="ExternalLinkService">
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<mods:mods id="0E59D52C1ACE61A2CF8322DC840BE33D" xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo id="5A66E0908F12AA14744F10C2D6C031E1">
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<mods:title id="F1BABAA15F4417EACAF8EB392247C733">Increasing diversity of land planarians (Platyhelminthes: Geoplanidae) in the Interior Atlantic Forest with the description of two new species and new records from Argentina</mods:title>
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<mods:name id="F17A0B5697987FFA0A3DC642F70532FA" type="personal">
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<mods:roleTerm id="3EBD49A972DB6B0E39C5B5B72662161C">Author</mods:roleTerm>
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<mods:namePart id="4D95334BF495F077E3C14D02E718FDA8">Negrete, Lisandro</mods:namePart>
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</mods:name>
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<mods:name id="C5F2702CB8D57C378306881012E92315" type="personal">
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<mods:roleTerm id="F71EC58AB999C63CB147EFC27AFA2BEC">Author</mods:roleTerm>
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</mods:role>
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<mods:namePart id="685DC51858676E3149F03A8850C57DFD">Brusa, Francisco</mods:namePart>
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</mods:name>
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<mods:typeOfResource id="9D20FDECADABFE915B8ECE5FF3B2BF32">text</mods:typeOfResource>
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<mods:title id="2F8B2486859F3172E31703AD2FE0CDD6">Zootaxa</mods:title>
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<mods:part id="257A85C070C38FF6F21B709AD300E79B">
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<mods:date id="1346A6DAFECF31A48133649132C2A15F">2017</mods:date>
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<mods:number id="27490783D11367D066109C434C2B9454">2017-12-04</mods:number>
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<mods:detail id="EAFD20E49C0F836147B52A4595E383BD" type="volume">
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<mods:number id="7DC173C34704434C77A282421E9735E8">4362</mods:number>
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<mods:detail id="628CD087C993437404BCD5EC88B93088" type="issue">
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<mods:number id="FCA01D4F80C64BC8A5BFF00EF57722EE">1</mods:number>
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<mods:classification id="74A39F658820657608DF5FC6097BCF40">journal article</mods:classification>
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<mods:identifier id="3E8F04338F53C72BCB14FD07357D2FD1" type="CLB-Dataset">31251</mods:identifier>
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<mods:identifier id="70B33E877F6051B86DB497A4BDBEC801" type="DOI">10.11646/zootaxa.4362.1.5</mods:identifier>
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<mods:identifier id="406EE438E54B2304E230FA5BE3BF5045" type="GBIF-Dataset">99c9eb85-21bc-470b-beda-e3f5fd023404</mods:identifier>
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<mods:identifier id="3895FB54E9260C44AED0E71CEEAF9DDC" type="ISSN">1175-5326</mods:identifier>
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<mods:identifier id="01F2E765A8E0B84693F692F4C17300CE" type="Zenodo-Dep">1076306</mods:identifier>
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</mods:mods>
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<treatment id="0397BB6CFFF9FFCDBDBE92CAC8E5FC08" ID-DOI="http://doi.org/10.5281/zenodo.6042420" ID-GBIF-Taxon="137584797" ID-Zenodo-Dep="6042420" LSID="urn:lsid:plazi:treatment:0397BB6CFFF9FFCDBDBE92CAC8E5FC08" httpUri="http://treatment.plazi.org/id/0397BB6CFFF9FFCDBDBE92CAC8E5FC08" lastPageId="13" lastPageNumber="112" pageId="7" pageNumber="106">
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<subSubSection id="C32459F1FFF9FFC7BDBE92CACCF4FDB1" pageId="7" pageNumber="106" type="nomenclature">
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<paragraph id="8B810A7AFFF9FFC7BDBE92CACCC2FD90" blockId="7.[151,428,510,570]" box="[151,428,510,536]" pageId="7" pageNumber="106">
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<heading id="D0C9BD16FFF9FFC7BDBE92CACCC2FD90" bold="true" box="[151,428,510,536]" fontSize="11" level="1" pageId="7" pageNumber="106" reason="1">
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<emphasis id="B94AD668FFF9FFC7BDBE92CACCC2FD90" bold="true" box="[151,428,510,536]" pageId="7" pageNumber="106">
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<taxonomicName id="4C3E71F9FFF9FFC7BDBE92CACC29FD90" authority="Negrete & Brusa, 2017" authorityName="Negrete & Brusa" authorityYear="2017" box="[151,327,510,536]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="7" pageNumber="106" phylum="Platyhelminthes" rank="species" species="negrita" status="sp. nov.">
|
||
<emphasis id="B94AD668FFF9FFC7BDBE92CACC29FD90" bold="true" box="[151,327,510,536]" italics="true" pageId="7" pageNumber="106">Imbira negrita</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A2796B13FFF9FFC7BC6792CACCC2FD90" box="[334,428,510,536]" pageId="7" pageNumber="106" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="8B810A7AFFF9FFC7BDBE9115CCF4FDB1" blockId="7.[151,428,510,570]" box="[151,410,545,570]" pageId="7" pageNumber="106">
|
||
(
|
||
<figureCitation id="130516FFFFF9FFC7BDB69115CC65FDB2" box="[159,267,545,570]" captionStart-0="FIGURE 4" captionStart-1="FIGURE 5" captionStart-2="FIGURE 6" captionStartId-0="9.[151,249,1834,1856]" captionStartId-1="10.[151,249,1686,1708]" captionStartId-2="12.[151,249,1434,1456]" captionTargetBox-0="[249,1350,342,1810]" captionTargetBox-1="[254,1337,197,1646]" captionTargetBox-2="[254,1331,199,1400]" captionTargetId-0="figure@9.[234,1353,338,1812]" captionTargetId-1="figure@10.[246,1340,193,1664]" captionTargetId-2="figure@12.[248,1338,193,1412]" captionTargetPageId-0="9" captionTargetPageId-1="10" captionTargetPageId-2="12" captionText-0="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." captionText-1="FIGURE 5. Imbira negrita sp. nov. (holotype). (A) Sagittal section of the pharynx. (B–D) Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm." captionText-2="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri-0="https://zenodo.org/record/1076328/files/figure.png" httpUri-1="https://zenodo.org/record/1076330/files/figure.png" httpUri-2="https://zenodo.org/record/1076334/files/figure.png" pageId="7" pageNumber="106">Figs. 4–6</figureCitation>
|
||
,
|
||
<tableCitation id="C6BC3FC1FFF9FFC7BC3F9115CC1AFDB1" box="[278,372,545,570]" captionStart="TABLE 1" captionStartId="8.[151,239,152,175]" captionText="TABLE 1. Measurements (mm) from fixed specimens of Imbira negrita sp. nov. CS: width of creeping sole; DG: distance from gonopore to anterior end; DM: distance from mouth to anterior end. The numbers given in parentheses represent the position relative to body length (%). Thickness (µm) of cutaneous (CM) and parenchymal (PM) musculatures at the pre-pharyngeal region. CMI (cutaneous muscular index): ratio of height of cutaneous musculature to body height. PMI (parenchymal muscular index): ratio of height of parenchymal musculature to body height. Both indices measured at pre-pharyngeal region. (-) not measured." pageId="7" pageNumber="106">Tables 1</tableCitation>
|
||
,
|
||
<tableCitation id="C6BC3FC1FFF9FFC7BCAB9115CCFFFDB1" box="[386,401,545,569]" captionStart="TABLE 2" captionStartId="11.[151,239,362,385]" captionText="TABLE 2. Measurements (mm) of reproductive organs of Imbira negrita sp. nov. DPVP, distance between prostatic vesicle and pharyngeal pouch; LCOD, length of common glandular ovovitelline duct; LFA, length of female atrium; LFC, length of female canal; LMA, length of male atrium; LPP, length of penis papilla; LPV, length of prostatic vesicle. The numbers given in parentheses represent the position relative to body length (%)." pageId="7" pageNumber="106">2</tableCitation>
|
||
)
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C32459F1FFF9FFC7BDBE915DCE5AFC62" pageId="7" pageNumber="106" type="materials_examined">
|
||
<paragraph id="8B810A7AFFF9FFC7BDBE915DCE5AFC62" blockId="7.[151,1437,617,2010]" pageId="7" pageNumber="106">
|
||
<emphasis id="B94AD668FFF9FFC7BDBE915DCC2AFD0A" bold="true" box="[151,324,617,642]" pageId="7" pageNumber="106">Type material.</emphasis>
|
||
Holotype. MLP–He 7415, Moconá Provincial Park,
|
||
<date id="FF802CBAFFF9FFC7BEB8915DC930FD0A" box="[913,1118,617,642]" pageId="7" pageNumber="106" value="2015-11-06">6 November 2015</date>
|
||
; cephalic region: transversal sections on 21 slides; anterior region at the level of the ovaries: sagittal sections on 46 slides; pre-pharyngeal region: transverse sections on 6 slides; pharyngeal region: sagittal sections on 30 slides; post-pharyngeal region: sagittal sections on 17 slides; copulatory apparatus: sagittal sections on 25 slides.
|
||
<emphasis id="B94AD668FFF9FFC7B96F91E2C9AAFD65" box="[1094,1220,725,749]" italics="true" pageId="7" pageNumber="106">Paratype 1</emphasis>
|
||
, MLP–He 7416-1, Moconá Provincial Park,
|
||
<date id="FF802CBAFFF9FFC7BC9291CDCFE2FC9A" box="[443,652,761,786]" pageId="7" pageNumber="106" value="2015-11-06">6 November 2015</date>
|
||
; cephalic region: transversal sections on 17 slides; anterior region at the level of the ovaries: sagittal sections on 24 slides; pre-pharyngeal region: transverse sections on 6 slides; pharyngeal region: sagittal sections on 13 slides; copulatory apparatus: sagittal sections on 12 slides.
|
||
<emphasis id="B94AD668FFF9FFC7B8319076C8FBFCD1" box="[1304,1429,833,857]" italics="true" pageId="7" pageNumber="106">Paratype 2</emphasis>
|
||
, MLP–He 7416-2, Moconá Provincial Park,
|
||
<date id="FF802CBAFFF9FFC7BFA39051CE35FCF6" box="[650,859,869,894]" pageId="7" pageNumber="106" value="2015-11-06">6 November 2015</date>
|
||
; cephalic region: transversal sections on 15 slides; anterior region at the level of the ovaries: sagittal sections on 21 slides; pre-pharyngeal region: transverse sections on 6 slides; pharynx and copulatory apparatus: sagittal sections on 16 slides.
|
||
<emphasis id="B94AD668FFF9FFC7BED7909AC914FC4D" box="[1022,1146,941,965]" italics="true" pageId="7" pageNumber="106">Paratype 3</emphasis>
|
||
, MLP–He 7417, Moconá Provincial Park,
|
||
<date id="FF802CBAFFF9FFC7BC7B90E6CF6DFC62" box="[338,515,977,1002]" pageId="7" pageNumber="106" value="2016-10-08">8 October 2016</date>
|
||
; preserved in 70% ethanol.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C32459F1FFF9FFC7BDEE90C1C9EDFBDD" pageId="7" pageNumber="106" type="diagnosis">
|
||
<paragraph id="8B810A7AFFF9FFC7BDEE90C1C9EDFBDD" blockId="7.[151,1437,617,2010]" pageId="7" pageNumber="106">
|
||
<emphasis id="B94AD668FFF9FFC7BDEE90C1CC2FFB86" bold="true" box="[199,321,1013,1038]" pageId="7" pageNumber="106">Diagnosis.</emphasis>
|
||
Elongated body with parallel margins;
|
||
<quantity id="4CC6A79FFFF9FFC7BFD790C1CE13FB86" box="[766,893,1013,1038]" metricMagnitude="-2" metricUnit="m" metricValue="8.0" metricValueMax="11.0" metricValueMin="5.0" pageId="7" pageNumber="106" unit="mm" value="80.0" valueMax="110.0" valueMin="50.0">50–110mm</quantity>
|
||
in length in maximum extension; dorsal surface jet black and ventral surface ivory; eyes marginal along the body; short female canal opening in the postero-dorsal portion of the female atrium; epithelial lining of female atrium with stratified appearance.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C32459F1FFF9FFC7BDEE9755C813FBF2" box="[199,1405,1121,1146]" pageId="7" pageNumber="106" type="materials_examined">
|
||
<paragraph id="8B810A7AFFF9FFC7BDEE9755C813FBF2" blockId="7.[151,1437,617,2010]" box="[199,1405,1121,1146]" pageId="7" pageNumber="106">
|
||
<materialsCitation id="3B560027FFF9FFC7BDEE9755C813FBF2" ID-GBIF-Occurrence="1703235509" box="[199,1405,1121,1146]" country="Argentina" latitude="-27.14889" location="Misiones province" longLatPrecision="20" longitude="-53.898335" pageId="7" pageNumber="106" specimenCount="1" stateProvince="Misiones province" typeStatus="holotype">
|
||
<emphasis id="B94AD668FFF9FFC7BDEE9755CC0BFBF2" bold="true" box="[199,357,1121,1146]" pageId="7" pageNumber="106">
|
||
<typeStatus id="5485B4D8FFF9FFC7BDEE9755CC6CFBF2" box="[199,258,1121,1146]" pageId="7" pageNumber="106">Type</typeStatus>
|
||
locality.
|
||
</emphasis>
|
||
Moconá Provincial Park (
|
||
<geoCoordinate id="EE0A6CBDFFF9FFC7BFA59755CE76FBF2" box="[652,792,1121,1146]" direction="south" orientation="latitude" pageId="7" pageNumber="106" precision="15" value="-27.14889">27°08’56’’S</geoCoordinate>
|
||
,
|
||
<geoCoordinate id="EE0A6CBDFFF9FFC7BE0A9755CED7FBF2" box="[803,953,1121,1146]" direction="west" orientation="longitude" pageId="7" pageNumber="106" precision="15" value="-53.898335">53°53’54’’W</geoCoordinate>
|
||
),
|
||
<collectingRegion id="49FAC498FFF9FFC7BEE79755C9CEFBF2" box="[974,1184,1121,1146]" country="Argentina" name="Misiones" pageId="7" pageNumber="106">Misiones province</collectingRegion>
|
||
,
|
||
<collectingCountry id="F3294AEAFFF9FFC7B9859755C875FBF2" box="[1196,1307,1121,1146]" name="Argentina" pageId="7" pageNumber="106">Argentina</collectingCountry>
|
||
(
|
||
<figureCitation id="130516FFFFF9FFC7B8039755C802FBF2" box="[1322,1388,1121,1146]" captionStart="FIGURE 1" captionStartId="1.[151,249,1866,1888]" captionTargetBox="[151,1423,878,1841]" captionTargetId="figure@1.[151,1436,878,1841]" captionTargetPageId="1" captionText="FIGURE 1. Map with the known distribution of Geoplana quagga, Obama ladislavii, and Paraba multicolor throughout Brazil (original extension of the Atlantic Forest in dark grey). In detail, the Argentine portion of the Atlantic Forest (Misiones province) with the new records of these species and localities of the new species here described. (CIAR) Centro de Investigaciones Antonia Ramos, (MO) Moconá Provincial Park, (OS) Osununú Private Reserve, (SA) San Antonio, (SE) Salto Encantado Provincial Park." httpUri="https://zenodo.org/record/1076310/files/figure.png" pageId="7" pageNumber="106">Fig. 1</figureCitation>
|
||
).
|
||
</materialsCitation>
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C32459F1FFF9FFC7BDEE97B1CFFBFB6E" pageId="7" pageNumber="106" type="etymology">
|
||
<paragraph id="8B810A7AFFF9FFC7BDEE97B1CFFBFB6E" blockId="7.[151,1437,617,2010]" pageId="7" pageNumber="106">
|
||
<emphasis id="B94AD668FFF9FFC7BDEE97B1CC20FB16" bold="true" box="[199,334,1157,1182]" pageId="7" pageNumber="106">Etymology.</emphasis>
|
||
The species name alludes to the black pigmentation of the dorsum (black=negra/o in Spanish), since in
|
||
<collectingCountry id="F3294AEAFFF9FFC7BDD6979DCC01FB4A" box="[255,367,1193,1218]" name="Argentina" pageId="7" pageNumber="106">Argentina</collectingCountry>
|
||
and other south American Spanish-speaking countries,
|
||
<taxonomicName id="4C3E71F9FFF9FFC7B92C979EC938FB49" authorityName="Negrete & Brusa" authorityYear="2017" box="[1029,1110,1194,1217]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="7" pageNumber="106" phylum="Platyhelminthes" rank="species" species="negrita">
|
||
<emphasis id="B94AD668FFF9FFC7B92C979EC938FB49" box="[1029,1110,1194,1217]" italics="true" pageId="7" pageNumber="106">negrita</emphasis>
|
||
</taxonomicName>
|
||
is a colloquial way to call someone or something black or very obscure.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C32459F1FFF9FFCDBDEE97C5C8E5FC08" lastPageId="13" lastPageNumber="112" pageId="7" pageNumber="106" type="description">
|
||
<paragraph id="8B810A7AFFF9FFC7BDEE97C5C870F9A2" blockId="7.[151,1437,617,2010]" pageId="7" pageNumber="106">
|
||
<emphasis id="B94AD668FFF9FFC7BDEE97C5CC37FA82" bold="true" box="[199,345,1265,1290]" pageId="7" pageNumber="106">Description.</emphasis>
|
||
<emphasis id="B94AD668FFF9FFC7BC4897C5CF39FA81" box="[353,599,1265,1289]" italics="true" pageId="7" pageNumber="106">External morphology.</emphasis>
|
||
Body elongate, with parallel margins, and flattened in cross section (
|
||
<figureCitation id="130516FFFFF9FFC7B84697C5CDB0FAA6" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="7" pageNumber="106">Fig. 4A–C</figureCitation>
|
||
). Anterior tip blunt, and posterior body region gradually sharpening, with pointed end (
|
||
<figureCitation id="130516FFFFF9FFC7B9F49621C855FAA6" box="[1245,1339,1301,1326]" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="7" pageNumber="106">Fig. 4A</figureCitation>
|
||
). Dorsal surface jet black and ventral surface ivory, only whitish around the mouth and gonopore (
|
||
<figureCitation id="130516FFFFF9FFC7B9A2960DC989FADA" box="[1163,1255,1337,1362]" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="7" pageNumber="106">Fig. 4A</figureCitation>
|
||
). After fixation, dorsal ground colour black grey and ventral surface oyster white. Eyes, with small clear halos, exclusively marginal, being uniserial throughout the body, except in the middle third, where they are irregularly bi-serial. When crawling, the maximum length of the largest specimen was
|
||
<quantity id="4CC6A79FFFF9FFC7BE159692CEFDFA36" box="[828,915,1446,1470]" metricMagnitude="-1" metricUnit="m" metricValue="1.1" pageId="7" pageNumber="106" unit="mm" value="110.0">110mm</quantity>
|
||
, whereas that after fixation was
|
||
<quantity id="4CC6A79FFFF9FFC7B82C9691C80BFA36" box="[1285,1381,1445,1470]" metricMagnitude="-2" metricUnit="m" metricValue="9.05" pageId="7" pageNumber="106" unit="mm" value="90.5">90.5mm</quantity>
|
||
. The maximum length of the smallest specimen was
|
||
<quantity id="4CC6A79FFFF9FFC7BF9896FDCE60FA6A" box="[689,782,1481,1506]" metricMagnitude="-2" metricUnit="m" metricValue="3.6399999999999997" pageId="7" pageNumber="106" unit="mm" value="36.4">36.4mm</quantity>
|
||
after fixation (
|
||
<tableCitation id="C6BC3FC1FFF9FFC7BE9196FDC963FA6A" box="[952,1037,1481,1506]" captionStart="TABLE 1" captionStartId="8.[151,239,152,175]" captionText="TABLE 1. Measurements (mm) from fixed specimens of Imbira negrita sp. nov. CS: width of creeping sole; DG: distance from gonopore to anterior end; DM: distance from mouth to anterior end. The numbers given in parentheses represent the position relative to body length (%). Thickness (µm) of cutaneous (CM) and parenchymal (PM) musculatures at the pre-pharyngeal region. CMI (cutaneous muscular index): ratio of height of cutaneous musculature to body height. PMI (parenchymal muscular index): ratio of height of parenchymal musculature to body height. Both indices measured at pre-pharyngeal region. (-) not measured." pageId="7" pageNumber="106">Table 1</tableCitation>
|
||
). Maximum width ranged between
|
||
<quantity id="4CC6A79FFFF9FFC7BDBE96DACD88F98E" box="[151,230,1518,1542]" metricMagnitude="-3" metricUnit="m" metricValue="2.9" pageId="7" pageNumber="106" unit="mm" value="2.9">2.9mm</quantity>
|
||
and
|
||
<quantity id="4CC6A79FFFF9FFC7BC0A96D9CC1AF98E" box="[291,372,1517,1542]" metricMagnitude="-3" metricUnit="m" metricValue="5.3" pageId="7" pageNumber="106" unit="mm" value="5.3">5.3mm</quantity>
|
||
, whereas maximum height ranged between 1 and
|
||
<quantity id="4CC6A79FFFF9FFC7BE9696D9C97FF98E" box="[959,1041,1517,1542]" metricMagnitude="-3" metricUnit="m" metricValue="1.6" pageId="7" pageNumber="106" unit="mm" value="1.6">1.6mm</quantity>
|
||
. The mouth and the gonopore are located at 71–81% (mean: 76%) and 88–93% (mean: 90%) from the anterior tip, respectively (
|
||
<tableCitation id="C6BC3FC1FFF9FFC7B9909525C863F9A2" box="[1209,1293,1553,1578]" captionStart="TABLE 1" captionStartId="8.[151,239,152,175]" captionText="TABLE 1. Measurements (mm) from fixed specimens of Imbira negrita sp. nov. CS: width of creeping sole; DG: distance from gonopore to anterior end; DM: distance from mouth to anterior end. The numbers given in parentheses represent the position relative to body length (%). Thickness (µm) of cutaneous (CM) and parenchymal (PM) musculatures at the pre-pharyngeal region. CMI (cutaneous muscular index): ratio of height of cutaneous musculature to body height. PMI (parenchymal muscular index): ratio of height of parenchymal musculature to body height. Both indices measured at pre-pharyngeal region. (-) not measured." pageId="7" pageNumber="106">Table 1</tableCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8B810A7AFFF9FFC7BDEE9501CC0BF852" blockId="7.[151,1437,617,2010]" pageId="7" pageNumber="106">
|
||
<emphasis id="B94AD668FFF9FFC7BDEE9501CCD9F9C5" box="[199,439,1589,1613]" italics="true" pageId="7" pageNumber="106">Internal morphology.</emphasis>
|
||
Sensory pits (30–50µm deep), as simple invaginations of ventral epidermis, contour the anterior tip and extend along the body margins in a single irregular row until ~
|
||
<quantity id="4CC6A79FFFF9FFC7BED5956DC958F9FA" box="[1020,1078,1625,1650]" metricMagnitude="-3" metricUnit="m" metricValue="5.0" pageId="7" pageNumber="106" unit="mm" value="5.0">5mm</quantity>
|
||
from the anterior end (
|
||
<figureCitation id="130516FFFFF9FFC7B81E956DC8E1F9FA" box="[1335,1423,1625,1650]" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="7" pageNumber="106">Fig. 4B</figureCitation>
|
||
). Sensory pits at intervals of 30–40µm near anterior tip, posteriorly becoming more gradually spaced. At the prepharyngeal region, the ventral epidermis (25–40µm high), which is ciliated on the creeping sole (~100% of body width at the pre-pharyngeal region), receives fine granular cyanophil secretion (more abundant in the ventro-lateral margins of the body), fine and coarse granular erythrophil secretions, and coarse granular xanthophil secretion. Cell bodies of these secretions located in the subjacent parenchyma, below cutaneous musculature (
|
||
<figureCitation id="130516FFFFF9FFC7B83A9439C8E1F8AE" box="[1299,1423,1805,1830]" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="7" pageNumber="106">Fig. 4C, E</figureCitation>
|
||
). Additionally, small rhabdites are highly abundant. The same
|
||
<typeStatus id="5485B4D8FFF9FFC7BE649406CEE6F8C2" box="[845,904,1842,1866]" pageId="7" pageNumber="106">types</typeStatus>
|
||
of secretory cells discharge through the dorsal epidermis (20–25µm high), with rhammites covering the whole dorsal epidermis (
|
||
<figureCitation id="130516FFFFF9FFC7B9469461C999F8E6" box="[1135,1271,1877,1902]" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="7" pageNumber="106">Fig. 4C, D</figureCitation>
|
||
). There is no glandular margin. The dorsal and ventral epidermis of the cephalic region receives the same
|
||
<typeStatus id="5485B4D8FFF9FFC7B9B5944EC9B9F81A" box="[1180,1239,1914,1938]" pageId="7" pageNumber="106">types</typeStatus>
|
||
of secretory cells as the pre-pharyngeal region, except xanthophil secretion. The creeping sole of the cephalic region occupies ~85% of the body width.
|
||
</paragraph>
|
||
<caption id="DF415AF2FFF6FFC8BDBE93ACCE4FFEC7" pageId="8" pageNumber="107">
|
||
<paragraph id="8B810A7AFFF6FFC8BDBE93ACCE4FFEC7" blockId="8.[151,1436,151,335]" pageId="8" pageNumber="107">
|
||
<emphasis id="B94AD668FFF6FFC8BDBE93ACCC60FF27" bold="true" box="[151,270,152,175]" pageId="8" pageNumber="107">TABLE 1.</emphasis>
|
||
Measurements (mm) from fixed specimens of
|
||
<taxonomicName id="4C3E71F9FFF6FFC8BE0993A3CEAEFF27" authority="Negrete & Brusa, 2017" authorityName="Negrete & Brusa" authorityYear="2017" box="[800,960,151,175]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="8" pageNumber="107" phylum="Platyhelminthes" rank="species" species="negrita" status="sp. nov.">
|
||
<emphasis id="B94AD668FFF6FFC8BE0993A3CEAEFF27" box="[800,960,151,175]" italics="true" pageId="8" pageNumber="107">Imbira negrita</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A2796B13FFF6FFC8BEE293ACC94BFF27" box="[971,1061,152,175]" pageId="8" pageNumber="107" rank="species">
|
||
<emphasis id="B94AD668FFF6FFC8BEE293ACC94BFF27" bold="true" box="[971,1061,152,175]" pageId="8" pageNumber="107">sp. nov.</emphasis>
|
||
</taxonomicNameLabel>
|
||
CS: width of creeping sole; DG: distance from gonopore to anterior end; DM: distance from mouth to anterior end. The numbers given in parentheses represent the position relative to body length (%). Thickness (µm) of cutaneous (CM) and parenchymal (PM) musculatures at the pre-pharyngeal region. CMI (cutaneous muscular index): ratio of height of cutaneous musculature to body height. PMI (parenchymal muscular index): ratio of height of parenchymal musculature to body height. Both indices measured at pre-pharyngeal region. (-) not measured.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8B810A7AFFF6FFC8BCA4925EC9C4FCCF" pageId="8" pageNumber="107">
|
||
<table id="F93EF8DAFFF6003FBDB6925EC860FCCF" box="[159,1294,362,839]" gridcols="5" gridrows="12" pageId="8" pageNumber="107">
|
||
<tr id="350E0838FFF6003FBDB6925EC860FE08" box="[159,1294,362,384]" gridrow="0" pageId="8" pageNumber="107" rowspan-0="1">
|
||
<th id="76DF6144FFF6003FBCA4925ECC83FE08" box="[397,493,362,384]" gridcol="1" gridrow="0" pageId="8" pageNumber="107">Holotype</th>
|
||
<th id="76DF6144FFF6003FBFBD925ECE68FE08" box="[660,774,362,384]" gridcol="2" gridrow="0" pageId="8" pageNumber="107">Paratype 1</th>
|
||
<th id="76DF6144FFF6003FBEB2925EC963FE08" box="[923,1037,362,384]" gridcol="3" gridrow="0" pageId="8" pageNumber="107">Paratype 2</th>
|
||
<th id="76DF6144FFF6003FB98B925EC860FE08" box="[1186,1294,362,384]" gridcol="4" gridrow="0" pageId="8" pageNumber="107">Paratype 3</th>
|
||
</tr>
|
||
<tr id="350E0838FFF6003FBDB692A0C860FE22" box="[159,1294,404,426]" gridrow="1" pageId="8" pageNumber="107">
|
||
<th id="76DF6144FFF6003FBDB692A0CC7CFE22" box="[159,274,404,426]" gridcol="0" gridrow="1" pageId="8" pageNumber="107">Length</th>
|
||
<td id="76DF6144FFF6003FBCA492A0CC83FE22" box="[397,493,404,426]" gridcol="1" gridrow="1" pageId="8" pageNumber="107">90.5</td>
|
||
<td id="76DF6144FFF6003FBFBD92A0CE68FE22" box="[660,774,404,426]" gridcol="2" gridrow="1" pageId="8" pageNumber="107">55.7</td>
|
||
<td id="76DF6144FFF6003FBEB292A0C963FE22" box="[923,1037,404,426]" gridcol="3" gridrow="1" pageId="8" pageNumber="107">36.4</td>
|
||
<td id="76DF6144FFF6003FB98B92A0C860FE22" box="[1186,1294,404,426]" gridcol="4" gridrow="1" pageId="8" pageNumber="107">80</td>
|
||
</tr>
|
||
<tr id="350E0838FFF6003FBDB69289C860FE5B" box="[159,1294,445,467]" gridrow="2" pageId="8" pageNumber="107">
|
||
<th id="76DF6144FFF6003FBDB69289CC7CFE5B" box="[159,274,445,467]" gridcol="0" gridrow="2" pageId="8" pageNumber="107">Width</th>
|
||
<td id="76DF6144FFF6003FBCA49289CC83FE5B" box="[397,493,445,467]" gridcol="1" gridrow="2" pageId="8" pageNumber="107">5.3</td>
|
||
<td id="76DF6144FFF6003FBFBD9289CE68FE5B" box="[660,774,445,467]" gridcol="2" gridrow="2" pageId="8" pageNumber="107">3.6</td>
|
||
<td id="76DF6144FFF6003FBEB29289C963FE5B" box="[923,1037,445,467]" gridcol="3" gridrow="2" pageId="8" pageNumber="107">2.9</td>
|
||
<td id="76DF6144FFF6003FB98B9289C860FE5B" box="[1186,1294,445,467]" gridcol="4" gridrow="2" pageId="8" pageNumber="107">5</td>
|
||
</tr>
|
||
<tr id="350E0838FFF6003FBDB692D2C860FE74" box="[159,1294,486,508]" gridrow="3" pageId="8" pageNumber="107">
|
||
<th id="76DF6144FFF6003FBDB692D2CC7CFE74" box="[159,274,486,508]" gridcol="0" gridrow="3" pageId="8" pageNumber="107">Height</th>
|
||
<td id="76DF6144FFF6003FBCA492D2CC83FE74" box="[397,493,486,508]" gridcol="1" gridrow="3" pageId="8" pageNumber="107">1.6</td>
|
||
<td id="76DF6144FFF6003FBFBD92D2CE68FE74" box="[660,774,486,508]" gridcol="2" gridrow="3" pageId="8" pageNumber="107">1.3</td>
|
||
<td id="76DF6144FFF6003FBEB292D2C963FE74" box="[923,1037,486,508]" gridcol="3" gridrow="3" pageId="8" pageNumber="107">1</td>
|
||
<td id="76DF6144FFF6003FB98B92D2C860FE74" box="[1186,1294,486,508]" gridcol="4" gridrow="3" pageId="8" pageNumber="107">-</td>
|
||
</tr>
|
||
<tr id="350E0838FFF6003FBDB69124C860FDAE" box="[159,1294,528,550]" gridrow="4" pageId="8" pageNumber="107">
|
||
<th id="76DF6144FFF6003FBDB69124CC7CFDAE" box="[159,274,528,550]" gridcol="0" gridrow="4" pageId="8" pageNumber="107">DM</th>
|
||
<td id="76DF6144FFF6003FBCA49124CC83FDAE" box="[397,493,528,550]" gridcol="1" gridrow="4" pageId="8" pageNumber="107">64 (71%)</td>
|
||
<td id="76DF6144FFF6003FBFBD9124CE68FDAE" box="[660,774,528,550]" gridcol="2" gridrow="4" pageId="8" pageNumber="107">45.1 (81%)</td>
|
||
<td id="76DF6144FFF6003FBEB29124C963FDAE" box="[923,1037,528,550]" gridcol="3" gridrow="4" pageId="8" pageNumber="107">27.8 (76%)</td>
|
||
<td id="76DF6144FFF6003FB98B9124C860FDAE" box="[1186,1294,528,550]" gridcol="4" gridrow="4" pageId="8" pageNumber="107">62 (77%)</td>
|
||
</tr>
|
||
<tr id="350E0838FFF6003FBDB6910DC860FDC7" box="[159,1294,569,591]" gridrow="5" pageId="8" pageNumber="107">
|
||
<th id="76DF6144FFF6003FBDB6910DCC7CFDC7" box="[159,274,569,591]" gridcol="0" gridrow="5" pageId="8" pageNumber="107">DG</th>
|
||
<td id="76DF6144FFF6003FBCA4910DCC83FDC7" box="[397,493,569,591]" gridcol="1" gridrow="5" pageId="8" pageNumber="107">80 (88%)</td>
|
||
<td id="76DF6144FFF6003FBFBD910DCE68FDC7" box="[660,774,569,591]" gridcol="2" gridrow="5" pageId="8" pageNumber="107">52.1 (93%)</td>
|
||
<td id="76DF6144FFF6003FBEB2910DC963FDC7" box="[923,1037,569,591]" gridcol="3" gridrow="5" pageId="8" pageNumber="107">31.9 (88%)</td>
|
||
<td id="76DF6144FFF6003FB98B910DC860FDC7" box="[1186,1294,569,591]" gridcol="4" gridrow="5" pageId="8" pageNumber="107">72 (90%)</td>
|
||
</tr>
|
||
<tr id="350E0838FFF6003FBDB69156C860FDF0" box="[159,1294,610,632]" gridrow="6" pageId="8" pageNumber="107">
|
||
<th id="76DF6144FFF6003FBDB69156CC7CFDF0" box="[159,274,610,632]" gridcol="0" gridrow="6" pageId="8" pageNumber="107">CS</th>
|
||
<td id="76DF6144FFF6003FBCA49156CC83FDF0" box="[397,493,610,632]" gridcol="1" gridrow="6" pageId="8" pageNumber="107">100%</td>
|
||
<td id="76DF6144FFF6003FBFBD9156CE68FDF0" box="[660,774,610,632]" gridcol="2" gridrow="6" pageId="8" pageNumber="107">100%</td>
|
||
<td id="76DF6144FFF6003FBEB29156C963FDF0" box="[923,1037,610,632]" gridcol="3" gridrow="6" pageId="8" pageNumber="107">90%</td>
|
||
<td id="76DF6144FFF6003FB98B9156C860FDF0" box="[1186,1294,610,632]" gridcol="4" gridrow="6" pageId="8" pageNumber="107">-</td>
|
||
</tr>
|
||
<tr id="350E0838FFF6003FBDB691B8C860FD2A" box="[159,1294,652,674]" gridrow="7" pageId="8" pageNumber="107">
|
||
<th id="76DF6144FFF6003FBDB691B8CC7CFD2A" box="[159,274,652,674]" gridcol="0" gridrow="7" pageId="8" pageNumber="107">CM dorsal</th>
|
||
<td id="76DF6144FFF6003FBCA491B8CC83FD2A" box="[397,493,652,674]" gridcol="1" gridrow="7" pageId="8" pageNumber="107">62.5</td>
|
||
<td id="76DF6144FFF6003FBFBD91B8CE68FD2A" box="[660,774,652,674]" gridcol="2" gridrow="7" pageId="8" pageNumber="107">65</td>
|
||
<td id="76DF6144FFF6003FBEB291B8C963FD2A" box="[923,1037,652,674]" gridcol="3" gridrow="7" pageId="8" pageNumber="107">62.5</td>
|
||
<td id="76DF6144FFF6003FB98B91B8C860FD2A" box="[1186,1294,652,674]" gridcol="4" gridrow="7" pageId="8" pageNumber="107">-</td>
|
||
</tr>
|
||
<tr id="350E0838FFF6003FBDB69181C860FD43" box="[159,1294,693,715]" gridrow="8" pageId="8" pageNumber="107">
|
||
<th id="76DF6144FFF6003FBDB69181CC7CFD43" box="[159,274,693,715]" gridcol="0" gridrow="8" pageId="8" pageNumber="107">CM ventral</th>
|
||
<td id="76DF6144FFF6003FBCA49181CC83FD43" box="[397,493,693,715]" gridcol="1" gridrow="8" pageId="8" pageNumber="107">75</td>
|
||
<td id="76DF6144FFF6003FBFBD9181CE68FD43" box="[660,774,693,715]" gridcol="2" gridrow="8" pageId="8" pageNumber="107">75</td>
|
||
<td id="76DF6144FFF6003FBEB29181C963FD43" box="[923,1037,693,715]" gridcol="3" gridrow="8" pageId="8" pageNumber="107">57.5</td>
|
||
<td id="76DF6144FFF6003FB98B9181C860FD43" box="[1186,1294,693,715]" gridcol="4" gridrow="8" pageId="8" pageNumber="107">-</td>
|
||
</tr>
|
||
<tr id="350E0838FFF6003FBDB691EAC860FD7C" box="[159,1294,734,756]" gridrow="9" pageId="8" pageNumber="107">
|
||
<th id="76DF6144FFF6003FBDB691EACC7CFD7C" box="[159,274,734,756]" gridcol="0" gridrow="9" pageId="8" pageNumber="107">CMI</th>
|
||
<td id="76DF6144FFF6003FBCA491EACC83FD7C" box="[397,493,734,756]" gridcol="1" gridrow="9" pageId="8" pageNumber="107">9%</td>
|
||
<td id="76DF6144FFF6003FBFBD91EACE68FD7C" box="[660,774,734,756]" gridcol="2" gridrow="9" pageId="8" pageNumber="107">11%</td>
|
||
<td id="76DF6144FFF6003FBEB291EAC963FD7C" box="[923,1037,734,756]" gridcol="3" gridrow="9" pageId="8" pageNumber="107">12%</td>
|
||
<td id="76DF6144FFF6003FB98B91EAC860FD7C" box="[1186,1294,734,756]" gridcol="4" gridrow="9" pageId="8" pageNumber="107">-</td>
|
||
</tr>
|
||
<tr id="350E0838FFF6003FBDB6903CC860FC96" box="[159,1294,776,798]" gridrow="10" pageId="8" pageNumber="107">
|
||
<th id="76DF6144FFF6003FBDB6903CCC7CFC96" box="[159,274,776,798]" gridcol="0" gridrow="10" pageId="8" pageNumber="107">PM</th>
|
||
<td id="76DF6144FFF6003FBCA4903CCC83FC96" box="[397,493,776,798]" gridcol="1" gridrow="10" pageId="8" pageNumber="107">90</td>
|
||
<td id="76DF6144FFF6003FBFBD903CCE68FC96" box="[660,774,776,798]" gridcol="2" gridrow="10" pageId="8" pageNumber="107">95</td>
|
||
<td id="76DF6144FFF6003FBEB2903CC963FC96" box="[923,1037,776,798]" gridcol="3" gridrow="10" pageId="8" pageNumber="107">50</td>
|
||
<td id="76DF6144FFF6003FB98B903CC860FC96" box="[1186,1294,776,798]" gridcol="4" gridrow="10" pageId="8" pageNumber="107">-</td>
|
||
</tr>
|
||
<tr id="350E0838FFF6003FBDB69005C860FCCF" box="[159,1294,817,839]" gridrow="11" pageId="8" pageNumber="107">
|
||
<th id="76DF6144FFF6003FBDB69005CC7CFCCF" box="[159,274,817,839]" gridcol="0" gridrow="11" pageId="8" pageNumber="107">PMI</th>
|
||
<td id="76DF6144FFF6003FBCA49005CC83FCCF" box="[397,493,817,839]" gridcol="1" gridrow="11" pageId="8" pageNumber="107">6%</td>
|
||
<td id="76DF6144FFF6003FBFBD9005CE68FCCF" box="[660,774,817,839]" gridcol="2" gridrow="11" pageId="8" pageNumber="107">7%</td>
|
||
<td id="76DF6144FFF6003FBEB29005C963FCCF" box="[923,1037,817,839]" gridcol="3" gridrow="11" pageId="8" pageNumber="107">5%</td>
|
||
<td id="76DF6144FFF6003FB98B9005C860FCCF" box="[1186,1294,817,839]" gridcol="4" gridrow="11" pageId="8" pageNumber="107">-</td>
|
||
</tr>
|
||
</table>
|
||
</paragraph>
|
||
<paragraph id="8B810A7AFFF6FFC8BDEE90B8CEC1FA84" blockId="8.[151,1437,908,2013]" pageId="8" pageNumber="107">
|
||
Cutaneous musculature composed of a subepidermal circular layer (2.5–5µm thick), an intermediate diagonal layer (5–15µm thick), and an inner longitudinal layer (50–60µm thick) (
|
||
<figureCitation id="130516FFFFF6FFC8BE90909BC95CFC40" box="[953,1074,943,968]" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="8" pageNumber="107">Fig. 4D, E</figureCitation>
|
||
). The thickness of the cutaneous musculature occupies 9–12% of the body height (
|
||
<tableCitation id="C6BC3FC1FFF6FFC8BFE590E0CE4FFC64" box="[716,801,980,1005]" captionStart="TABLE 1" captionStartId="8.[151,239,152,175]" captionText="TABLE 1. Measurements (mm) from fixed specimens of Imbira negrita sp. nov. CS: width of creeping sole; DG: distance from gonopore to anterior end; DM: distance from mouth to anterior end. The numbers given in parentheses represent the position relative to body length (%). Thickness (µm) of cutaneous (CM) and parenchymal (PM) musculatures at the pre-pharyngeal region. CMI (cutaneous muscular index): ratio of height of cutaneous musculature to body height. PMI (parenchymal muscular index): ratio of height of parenchymal musculature to body height. Both indices measured at pre-pharyngeal region. (-) not measured." pageId="8" pageNumber="107">Table 1</tableCitation>
|
||
). Parenchymal musculature constituted by a dorsal, not well organized, layer with oblique and some longitudinal fibres, followed by a transverse supra-intestinal and a sub-intestinal muscle layer (
|
||
<figureCitation id="130516FFFFF6FFC8BCF89728CF27FBBC" box="[465,585,1052,1077]" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="8" pageNumber="107">Fig. 4C–E</figureCitation>
|
||
). Longitudinal fibres (20–30µm thick) accommodate immediately above the sub-intestinal layer (
|
||
<figureCitation id="130516FFFFF6FFC8BCAA970BCCB0FBD0" box="[387,478,1087,1112]" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="8" pageNumber="107">Fig. 4E</figureCitation>
|
||
). Also, dorso-ventral fibres are arranged among intestinal branches (
|
||
<figureCitation id="130516FFFFF6FFC8B9D5970BC837FBD0" box="[1276,1369,1087,1112]" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="8" pageNumber="107">Fig. 4D</figureCitation>
|
||
). The parenchymal musculature occupies 5–7% of the body height (
|
||
<tableCitation id="C6BC3FC1FFF6FFC8BE639750CEF3FBF4" box="[842,925,1124,1149]" captionStart="TABLE 1" captionStartId="8.[151,239,152,175]" captionText="TABLE 1. Measurements (mm) from fixed specimens of Imbira negrita sp. nov. CS: width of creeping sole; DG: distance from gonopore to anterior end; DM: distance from mouth to anterior end. The numbers given in parentheses represent the position relative to body length (%). Thickness (µm) of cutaneous (CM) and parenchymal (PM) musculatures at the pre-pharyngeal region. CMI (cutaneous muscular index): ratio of height of cutaneous musculature to body height. PMI (parenchymal muscular index): ratio of height of parenchymal musculature to body height. Both indices measured at pre-pharyngeal region. (-) not measured." pageId="8" pageNumber="107">Table 1</tableCitation>
|
||
). In the cephalic region, and even at the level of the ovaries, parenchymal muscles exhibit the same arrangement as the pre-pharyngeal region, but longitudinal fibres are more abundant. They form discrete bundles (30–45µm thick) above the sub-intestinal muscle layer and below it mainly towards the body margins (
|
||
<figureCitation id="130516FFFFF6FFC8BFB497FBCE6AFB60" box="[669,772,1231,1256]" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="8" pageNumber="107">Figs. 4B</figureCitation>
|
||
,
|
||
<figureCitation id="130516FFFFF6FFC8BE3D97FBCE34FB60" box="[788,858,1231,1256]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="8" pageNumber="107">6F, G</figureCitation>
|
||
). Numerous parenchymal muscle fibres variously oriented traverse the nervous plate along the cephalic region (
|
||
<figureCitation id="130516FFFFF6FFC8BE6097C0CECCFA84" box="[841,930,1268,1293]" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="8" pageNumber="107">Fig. 4B</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8B810A7AFFF6FFC8BDEE9623C99AF9FD" blockId="8.[151,1437,908,2013]" pageId="8" pageNumber="107">
|
||
The pharynx is collar-type,
|
||
<quantity id="4CC6A79FFFF6FFC8BF2F9623CFE8FAB8" box="[518,646,1303,1328]" metricMagnitude="-3" metricUnit="m" metricValue="3.8499999999999996" metricValueMax="5.1" metricValueMin="2.6" pageId="8" pageNumber="107" unit="mm" value="3.8499999999999996" valueMax="5.1" valueMin="2.6">2.6–5.1mm</quantity>
|
||
in length (6–7% of body length), occupying most of the pharyngeal pouch (
|
||
<quantity id="4CC6A79FFFF6FFC8BDD99608CC1FFADD" box="[240,369,1340,1365]" metricMagnitude="-3" metricUnit="m" metricValue="4.4" metricValueMax="6.5" metricValueMin="2.3" pageId="8" pageNumber="107" unit="mm" value="4.4" valueMax="6.5" valueMin="2.3">2.3–6.5mm</quantity>
|
||
in length) (
|
||
<figureCitation id="130516FFFFF6FFC8BF2C9608CF0BFADD" box="[517,613,1340,1365]" captionStart="FIGURE 5" captionStartId="10.[151,249,1686,1708]" captionTargetBox="[254,1337,197,1646]" captionTargetId="figure@10.[246,1340,193,1664]" captionTargetPageId="10" captionText="FIGURE 5. Imbira negrita sp. nov. (holotype). (A) Sagittal section of the pharynx. (B–D) Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm." httpUri="https://zenodo.org/record/1076330/files/figure.png" pageId="8" pageNumber="107">Fig. 5A</figureCitation>
|
||
). Dorsal insertion is
|
||
<quantity id="4CC6A79FFFF6FFC8BE4C9608CE8BFADD" box="[869,997,1340,1365]" metricMagnitude="-3" metricUnit="m" metricValue="2.45" metricValueMax="3.5" metricValueMin="1.4" pageId="8" pageNumber="107" unit="mm" value="2.45" valueMax="3.5" valueMin="1.4">1.4–3.5mm</quantity>
|
||
posteriorly displaced (
|
||
<figureCitation id="130516FFFFF6FFC8B9DE9608C839FADD" box="[1271,1367,1340,1365]" captionStart="FIGURE 5" captionStartId="10.[151,249,1686,1708]" captionTargetBox="[254,1337,197,1646]" captionTargetId="figure@10.[246,1340,193,1664]" captionTargetPageId="10" captionText="FIGURE 5. Imbira negrita sp. nov. (holotype). (A) Sagittal section of the pharynx. (B–D) Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm." httpUri="https://zenodo.org/record/1076330/files/figure.png" pageId="8" pageNumber="107">Fig. 5A</figureCitation>
|
||
). The pharynx is lined by ciliated cuboidal epithelium, followed by a longitudinal muscle layer (2.5–5µm thick) and a subjacent circular muscle layer (5–10µm thick). Pharyngeal lumen lined with ciliated columnar epithelium, followed by a circular subepithelial muscle layer (50–75µm thick) and a subjacent longitudinal muscle layer (5– 10µm thick). Two
|
||
<typeStatus id="5485B4D8FFF6FFC8BC4496F9CCC6FA6D" box="[365,424,1485,1509]" pageId="8" pageNumber="107">types</typeStatus>
|
||
of secretory cells, whose cell bodies are located in the surrounding parenchyma anterior and lateral to the pharynx, discharge through the pharyngeal epithelium: highly abundant cells with fine granular erythrophil secretion and abundant fine granular cyanophil secretion (
|
||
<figureCitation id="130516FFFFF6FFC8BE999520C962F9A5" box="[944,1036,1556,1581]" captionStart="FIGURE 5" captionStartId="10.[151,249,1686,1708]" captionTargetBox="[254,1337,197,1646]" captionTargetId="figure@10.[246,1340,193,1664]" captionTargetPageId="10" captionText="FIGURE 5. Imbira negrita sp. nov. (holotype). (A) Sagittal section of the pharynx. (B–D) Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm." httpUri="https://zenodo.org/record/1076330/files/figure.png" pageId="8" pageNumber="107">Fig. 5A</figureCitation>
|
||
). Oesophagus (
|
||
<quantity id="4CC6A79FFFF6FFC8B9939520C848F9A4" box="[1210,1318,1556,1580]" metricMagnitude="-4" metricUnit="m" metricValue="7.0" metricValueMax="10.0" metricValueMin="4.0" pageId="8" pageNumber="107" unit="mm" value="0.7" valueMax="1.0" valueMin="0.4">0.4–1mm</quantity>
|
||
in length) lined with ciliated columnar epithelium, followed by a subepithelial circular muscle layer (100–130µm thick) and a thin subjacent longitudinal muscle layer (5–15µm thick). The oesophagus:pharynx ratio is 15–21%.
|
||
</paragraph>
|
||
<paragraph id="8B810A7AFFF6FFC9BDEE954BC9D0FE94" blockId="8.[151,1437,908,2013]" lastBlockId="9.[151,1437,151,285]" lastPageId="9" lastPageNumber="108" pageId="8" pageNumber="107">
|
||
The dorsal mature testes are ovoid in shape, arranged in one or two irregular rows on each side of the body. They are located below the supra-intestinal parenchymal muscle layer (
|
||
<figureCitation id="130516FFFFF6FFC8BEFB9590C93DF934" box="[978,1107,1700,1725]" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="8" pageNumber="107">Fig. 4C, D</figureCitation>
|
||
), extending from behind the ovaries to near the pharynx (17–33% and 67–76% of body length from the anterior tip, respectively) (
|
||
<tableCitation id="C6BC3FC1FFF6FFC8B81D95F3C8E1F968" box="[1332,1423,1735,1760]" captionStart="TABLE 2" captionStartId="11.[151,239,362,385]" captionText="TABLE 2. Measurements (mm) of reproductive organs of Imbira negrita sp. nov. DPVP, distance between prostatic vesicle and pharyngeal pouch; LCOD, length of common glandular ovovitelline duct; LFA, length of female atrium; LFC, length of female canal; LMA, length of male atrium; LPP, length of penis papilla; LPV, length of prostatic vesicle. The numbers given in parentheses represent the position relative to body length (%)." pageId="8" pageNumber="107">Table 2</tableCitation>
|
||
). They occupy 18–20% of the body height at the pre-pharyngeal region. Sperm ducts, dorsally and laterally displaced from the ovovitelline ducts, located among fibres of the sub-intestinal parenchymal muscle layer (
|
||
<figureCitation id="130516FFFFF6FFC8B846943BCDB2F8C4" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="8" pageNumber="107">Fig. 4C, E</figureCitation>
|
||
). Laterally and behind the pharynx, sperm ducts are slightly dilated and full of spermatozoa. Their distal courses turn to the sagittal plane to open ventro-laterally into the forked portion of the extrabulbar prostatic vesicle (
|
||
<figureCitation id="130516FFFFF6FFC8BDB69448CC49F81D" box="[159,295,1916,1941]" captionStart="FIGURE 5" captionStartId="10.[151,249,1686,1708]" captionTargetBox="[254,1337,197,1646]" captionTargetId="figure@10.[246,1340,193,1664]" captionTargetPageId="10" captionText="FIGURE 5. Imbira negrita sp. nov. (holotype). (A) Sagittal section of the pharynx. (B–D) Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm." httpUri="https://zenodo.org/record/1076330/files/figure.png" pageId="8" pageNumber="107">Figs. 5B–D</figureCitation>
|
||
,
|
||
<figureCitation id="130516FFFFF6FFC8BC1C9448CC35F81D" box="[309,347,1916,1941]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="8" pageNumber="107">6A</figureCitation>
|
||
). These long tubular paired portions (
|
||
<quantity id="4CC6A79FFFF6FFC8BE3A9448CE10F81C" box="[787,894,1916,1940]" metricMagnitude="-3" metricUnit="m" metricValue="1.6" metricValueMax="2.2" metricValueMin="1.0" pageId="8" pageNumber="107" unit="mm" value="1.6" valueMax="2.2" valueMin="1.0">1–2.2mm</quantity>
|
||
long) run laterally sinuous and continue as an unpaired intrabulbar portion whose course is also tortuous (
|
||
<quantity id="4CC6A79FFFF6FFC8BE7A9494CEBAF830" box="[851,980,1952,1976]" metricMagnitude="-4" metricUnit="m" metricValue="5.5" metricValueMax="7.0" metricValueMin="4.0" pageId="8" pageNumber="107" unit="mm" value="0.55" valueMax="0.7" valueMin="0.4">0.4–0.7mm</quantity>
|
||
long) (
|
||
<figureCitation id="130516FFFFF6FFC8B90794ABC9D6F830" box="[1070,1208,1951,1976]" captionStart="FIGURE 5" captionStartId="10.[151,249,1686,1708]" captionTargetBox="[254,1337,197,1646]" captionTargetId="figure@10.[246,1340,193,1664]" captionTargetPageId="10" captionText="FIGURE 5. Imbira negrita sp. nov. (holotype). (A) Sagittal section of the pharynx. (B–D) Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm." httpUri="https://zenodo.org/record/1076330/files/figure.png" pageId="8" pageNumber="107">Figs. 5B–D</figureCitation>
|
||
,
|
||
<figureCitation id="130516FFFFF6FFC8B9EE94ABC865F830" box="[1223,1291,1951,1976]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="8" pageNumber="107">6B–E</figureCitation>
|
||
). Very close before opening into the male atrium, the prostatic vesicle connects with the ejaculatory duct, which is a short canal with a narrow lumen (150–300µm long) (
|
||
<figureCitation id="130516FFFFF7FFC9BF5993A3CF99FF38" box="[624,759,151,176]" captionStart="FIGURE 5" captionStartId="10.[151,249,1686,1708]" captionTargetBox="[254,1337,197,1646]" captionTargetId="figure@10.[246,1340,193,1664]" captionTargetPageId="10" captionText="FIGURE 5. Imbira negrita sp. nov. (holotype). (A) Sagittal section of the pharynx. (B–D) Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm." httpUri="https://zenodo.org/record/1076330/files/figure.png" pageId="9" pageNumber="108">Figs. 5B–D</figureCitation>
|
||
,
|
||
<figureCitation id="130516FFFFF7FFC9BE2A93A3CE29FF38" box="[771,839,151,176]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="9" pageNumber="108">6C, E</figureCitation>
|
||
). Male atrium (
|
||
<quantity id="4CC6A79FFFF7FFC9BEDE93A3C916FF38" box="[1015,1144,151,176]" metricMagnitude="-3" metricUnit="m" metricValue="1.6" metricValueMax="2.5" metricValueMin="0.7" pageId="9" pageNumber="108" unit="mm" value="1.6" valueMax="2.5" valueMin="0.7">0.7–2.5mm</quantity>
|
||
long), with highly folded walls, leaving a reduced lumen inside the cavity (
|
||
<figureCitation id="130516FFFFF7FFC9BFED9388CE27FF5D" box="[708,841,188,213]" captionStart="FIGURE 5" captionStartId="10.[151,249,1686,1708]" captionTargetBox="[254,1337,197,1646]" captionTargetId="figure@10.[246,1340,193,1664]" captionTargetPageId="10" captionText="FIGURE 5. Imbira negrita sp. nov. (holotype). (A) Sagittal section of the pharynx. (B–D) Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm." httpUri="https://zenodo.org/record/1076330/files/figure.png" pageId="9" pageNumber="108">Figs. 5B–D</figureCitation>
|
||
,
|
||
<figureCitation id="130516FFFFF7FFC9BE7C9388CEF7FF5D" box="[853,921,188,213]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="9" pageNumber="108">6B–E</figureCitation>
|
||
). The folded walls of its proximal part, where the ejaculatory duct opens, conform a kind of papilla: the eversible penis (
|
||
<figureCitation id="130516FFFFF7FFC9B92A93EBC9E2FF70" box="[1027,1164,223,248]" captionStart="FIGURE 5" captionStartId="10.[151,249,1686,1708]" captionTargetBox="[254,1337,197,1646]" captionTargetId="figure@10.[246,1340,193,1664]" captionTargetPageId="10" captionText="FIGURE 5. Imbira negrita sp. nov. (holotype). (A) Sagittal section of the pharynx. (B–D) Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm." httpUri="https://zenodo.org/record/1076330/files/figure.png" pageId="9" pageNumber="108">Figs. 5B–D</figureCitation>
|
||
,
|
||
<figureCitation id="130516FFFFF7FFC9B9B293EBC9B1FF70" box="[1179,1247,223,248]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="9" pageNumber="108">6B–E</figureCitation>
|
||
). Only the male atrium of the
|
||
<typeStatus id="5485B4D8FFF7FFC9BC079230CCFEFE95" box="[302,400,260,285]" pageId="9" pageNumber="108" type="holotype">holotype</typeStatus>
|
||
exhibits dorsal folds posteriorly to the eversible penis (
|
||
<figureCitation id="130516FFFFF7FFC9BED79230C90EFE95" box="[1022,1120,260,285]" captionStart="FIGURE 5" captionStartId="10.[151,249,1686,1708]" captionTargetBox="[254,1337,197,1646]" captionTargetId="figure@10.[246,1340,193,1664]" captionTargetPageId="10" captionText="FIGURE 5. Imbira negrita sp. nov. (holotype). (A) Sagittal section of the pharynx. (B–D) Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm." httpUri="https://zenodo.org/record/1076330/files/figure.png" pageId="9" pageNumber="108">Figs. 5B</figureCitation>
|
||
,
|
||
<figureCitation id="130516FFFFF7FFC9B9459230C9DFFE94" box="[1132,1201,260,285]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="9" pageNumber="108">6B, C</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<caption id="DF415AF2FFF7FFC9BDBE941ECEDDF832" httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="9" pageNumber="108" targetBox="[249,1350,342,1810]" targetPageId="9">
|
||
<paragraph id="8B810A7AFFF7FFC9BDBE941ECEDDF832" blockId="9.[151,1436,1833,1978]" pageId="9" pageNumber="108">
|
||
<emphasis id="B94AD668FFF7FFC9BDBE941ECC78F8B7" bold="true" box="[151,278,1834,1856]" pageId="9" pageNumber="108">FIGURE 4.</emphasis>
|
||
<taxonomicName id="4C3E71F9FFF7FFC9BC36941ECCD9F8C8" authority="Negrete & Brusa, 2017" authorityName="Negrete & Brusa" authorityYear="2017" box="[287,439,1834,1856]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="9" pageNumber="108" phylum="Platyhelminthes" rank="species" species="negrita" status="sp. nov.">
|
||
<emphasis id="B94AD668FFF7FFC9BC36941ECCD9F8C8" box="[287,439,1834,1856]" italics="true" pageId="9" pageNumber="108">Imbira negrita</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="B94AD668FFF7FFC9BCE8941ECF2DF8C8" bold="true" box="[449,579,1833,1856]" pageId="9" pageNumber="108">
|
||
<taxonomicNameLabel id="A2796B13FFF7FFC9BCE8941ECF7BF8B7" box="[449,533,1834,1855]" pageId="9" pageNumber="108" rank="species">sp. nov.</taxonomicNameLabel>
|
||
(A)
|
||
</emphasis>
|
||
Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface.
|
||
<emphasis id="B94AD668FFF7FFC9BF58947CCFFDF8D7" bold="true" box="[625,659,1864,1887]" pageId="9" pageNumber="108">(B)</emphasis>
|
||
Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres.
|
||
<emphasis id="B94AD668FFF7FFC9B83B9453C84AF8F5" bold="true" box="[1298,1316,1895,1917]" pageId="9" pageNumber="108">C</emphasis>
|
||
Transverse section of the pre-pharyngeal region.
|
||
<emphasis id="B94AD668FFF7FFC9BF3094B1CF35F814" bold="true" box="[537,603,1925,1948]" pageId="9" pageNumber="108">(D, E)</emphasis>
|
||
Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars: A: 10mm, B, D, E: 250µm, C: 500µm.
|
||
</paragraph>
|
||
</caption>
|
||
<caption id="DF415AF2FFF4FFCABDBE95A2C829F942" httpUri="https://zenodo.org/record/1076330/files/figure.png" pageId="10" pageNumber="109" targetBox="[254,1337,197,1646]" targetPageId="10">
|
||
<paragraph id="8B810A7AFFF4FFCABDBE95A2C829F942" blockId="10.[151,1435,1685,1738]" pageId="10" pageNumber="109">
|
||
<emphasis id="B94AD668FFF4FFCABDBE95A2CC7DF923" bold="true" box="[151,275,1686,1708]" pageId="10" pageNumber="109">FIGURE 5.</emphasis>
|
||
<taxonomicName id="4C3E71F9FFF4FFCABC3595A2CCDCF924" authority="Negrete & Brusa, 2017" authorityName="Negrete & Brusa" authorityYear="2017" box="[284,434,1686,1708]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="10" pageNumber="109" phylum="Platyhelminthes" rank="species" species="negrita" status="sp. nov.">
|
||
<emphasis id="B94AD668FFF4FFCABC3595A2CCDCF924" box="[284,434,1686,1708]" italics="true" pageId="10" pageNumber="109">Imbira negrita</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A2796B13FFF4FFCABC9395A2CF62F923" box="[442,524,1686,1707]" pageId="10" pageNumber="109" rank="species">
|
||
<emphasis id="B94AD668FFF4FFCABC9395A2CF62F923" bold="true" box="[442,524,1686,1707]" pageId="10" pageNumber="109">sp. nov.</emphasis>
|
||
</taxonomicNameLabel>
|
||
(holotype).
|
||
<emphasis id="B94AD668FFF4FFCABFA495A1CFDEF924" bold="true" box="[653,688,1685,1708]" pageId="10" pageNumber="109">(A)</emphasis>
|
||
Sagittal section of the pharynx.
|
||
<emphasis id="B94AD668FFF4FFCAB92A95A1C92DF924" bold="true" box="[1027,1091,1685,1708]" pageId="10" pageNumber="109">(B–D)</emphasis>
|
||
Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8B810A7AFFF4FFCBBDEE95C0CCEEFEC8" blockId="10.[151,1437,1779,2020]" lastBlockId="11.[151,1436,151,320]" lastPageId="11" lastPageNumber="110" pageId="10" pageNumber="109">
|
||
Sperm ducts lined with ciliated cuboidal epithelium, without underlying musculature at pre-pharyngeal level. Epithelial lining of the prostatic vesicle, both paired and unpaired portions, columnar and ciliated, filled with abundant erythrophil fine granular secretion. Cell bodies of prostatic glands located in the surrounding parenchyma between the pharyngeal pouch and the prostatic vesicle (
|
||
<figureCitation id="130516FFFFF4FFCABE06946BCEE3F8F0" box="[815,909,1887,1912]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="10" pageNumber="109">Fig. 6A</figureCitation>
|
||
). Musculature of the prostatic vesicle mainly composed of longitudinal fibres with some interspersed oblique fibres (10–15µm thick). Ejaculatory duct lined with cuboidal to columnar ciliated epithelium, which is pierced by scarce erythrophil fine granules, surrounded by a thin longitudinal muscle layer (2.5µm thick). Epithelial lining of the male atrium columnar and densely ciliated, strongly erythrophil, receiving abundant fine granular secretion (
|
||
<figureCitation id="130516FFFFF5FFCBBEB293A3C974FF38" box="[923,1050,151,176]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="11" pageNumber="110">Fig. 6B–E</figureCitation>
|
||
). Its muscularis, composed of a subepithelial circular layer and a subjacent longitudinal layer, is thicker dorsally (20–30µm thick and 10–25µm thick, respectively) than ventrally (5–10µm thick and 5–10µm thick, respectively). Common muscle coat formed by loose longitudinal fibres (10–15µm thick). Male atrium connected with the female atrium by a narrow lumen (
|
||
<figureCitation id="130516FFFFF5FFCBBDB69213CC4AFEC8" box="[159,292,295,320]" captionStart="FIGURE 5" captionStartId="10.[151,249,1686,1708]" captionTargetBox="[254,1337,197,1646]" captionTargetId="figure@10.[246,1340,193,1664]" captionTargetPageId="10" captionText="FIGURE 5. Imbira negrita sp. nov. (holotype). (A) Sagittal section of the pharynx. (B–D) Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm." httpUri="https://zenodo.org/record/1076330/files/figure.png" pageId="11" pageNumber="110">Figs. 5B–D</figureCitation>
|
||
,
|
||
<figureCitation id="130516FFFFF5FFCBBC069213CC1AFEC8" box="[303,372,295,320]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="11" pageNumber="110">6C–E</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<caption id="DF415AF2FFF5FFCBBDBE925EC972FE69" pageId="11" pageNumber="110">
|
||
<paragraph id="8B810A7AFFF5FFCBBDBE925EC972FE69" blockId="11.[151,1436,361,481]" pageId="11" pageNumber="110">
|
||
<emphasis id="B94AD668FFF5FFCBBDBE925ECC65FE09" bold="true" box="[151,267,362,385]" pageId="11" pageNumber="110">TABLE 2.</emphasis>
|
||
Measurements (mm) of reproductive organs of
|
||
<taxonomicName id="4C3E71F9FFF5FFCBBE08925DCEAEFE09" authority="Negrete & Brusa, 2017" authorityName="Negrete & Brusa" authorityYear="2017" box="[801,960,361,385]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="11" pageNumber="110" phylum="Platyhelminthes" rank="species" species="negrita" status="sp. nov.">
|
||
<emphasis id="B94AD668FFF5FFCBBE08925DCEAEFE09" box="[801,960,361,385]" italics="true" pageId="11" pageNumber="110">Imbira negrita</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A2796B13FFF5FFCBBEE0925EC94CFE09" box="[969,1058,362,385]" pageId="11" pageNumber="110" rank="species">
|
||
<emphasis id="B94AD668FFF5FFCBBEE0925EC94CFE09" bold="true" box="[969,1058,362,385]" pageId="11" pageNumber="110">sp. nov.</emphasis>
|
||
</taxonomicNameLabel>
|
||
DPVP, distance between prostatic vesicle and pharyngeal pouch; LCOD, length of common glandular ovovitelline duct; LFA, length of female atrium; LFC, length of female canal; LMA, length of male atrium; LPP, length of penis papilla; LPV, length of prostatic vesicle. The numbers given in parentheses represent the position relative to body length (%).
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8B810A7AFFF5FFCBBF8592C9C9B9FC38" pageId="11" pageNumber="110">
|
||
<table id="F93EF8DAFFF5003FBDB692C9C875FC38" box="[159,1307,509,944]" gridcols="4" gridrows="11" pageId="11" pageNumber="110">
|
||
<tr id="350E0838FFF5003FBDB692C9C875FD9B" box="[159,1307,509,531]" gridrow="0" pageId="11" pageNumber="110" rowspan-0="1">
|
||
<th id="76DF6144FFF5003FBF8592C9CE70FD9B" box="[684,798,509,531]" gridcol="1" gridrow="0" pageId="11" pageNumber="110">Holotype</th>
|
||
<th id="76DF6144FFF5003FBE9892C9C94CFD9B" box="[945,1058,509,531]" gridcol="2" gridrow="0" pageId="11" pageNumber="110">Paratype 1</th>
|
||
<th id="76DF6144FFF5003FB98292C9C875FD9B" box="[1195,1307,509,531]" gridcol="3" gridrow="0" pageId="11" pageNumber="110">Paratype 2</th>
|
||
</tr>
|
||
<tr id="350E0838FFF5003FBDB69112C875FDB4" box="[159,1307,550,572]" gridrow="1" pageId="11" pageNumber="110">
|
||
<th id="76DF6144FFF5003FBDB69112CCC7FDB4" box="[159,425,550,572]" gridcol="0" gridrow="1" pageId="11" pageNumber="110">Anteriormost testes</th>
|
||
<td id="76DF6144FFF5003FBF859112CE70FDB4" box="[684,798,550,572]" gridcol="1" gridrow="1" pageId="11" pageNumber="110">15.2 (17%)</td>
|
||
<td id="76DF6144FFF5003FBE989112C94CFDB4" box="[945,1058,550,572]" gridcol="2" gridrow="1" pageId="11" pageNumber="110">9.9 (18%)</td>
|
||
<td id="76DF6144FFF5003FB9829112C875FDB4" box="[1195,1307,550,572]" gridcol="3" gridrow="1" pageId="11" pageNumber="110">12 (33%)</td>
|
||
</tr>
|
||
<tr id="350E0838FFF5003FBDB69164C875FDEE" box="[159,1307,592,614]" gridrow="2" pageId="11" pageNumber="110">
|
||
<th id="76DF6144FFF5003FBDB69164CCC7FDEE" box="[159,425,592,614]" gridcol="0" gridrow="2" pageId="11" pageNumber="110">Posteriormost testes</th>
|
||
<td id="76DF6144FFF5003FBF859164CE70FDEE" box="[684,798,592,614]" gridcol="1" gridrow="2" pageId="11" pageNumber="110">60.4 (67%)</td>
|
||
<td id="76DF6144FFF5003FBE989164C94CFDEE" box="[945,1058,592,614]" gridcol="2" gridrow="2" pageId="11" pageNumber="110">42.5 (76%)</td>
|
||
<td id="76DF6144FFF5003FB9829164C875FDEE" box="[1195,1307,592,614]" gridcol="3" gridrow="2" pageId="11" pageNumber="110">26.3 (72%)</td>
|
||
</tr>
|
||
<tr id="350E0838FFF5003FBDB6914DC875FD07" box="[159,1307,633,655]" gridrow="3" pageId="11" pageNumber="110">
|
||
<th id="76DF6144FFF5003FBDB6914DCCC7FD07" box="[159,425,633,655]" gridcol="0" gridrow="3" pageId="11" pageNumber="110">LPV (extrabulbar portion)</th>
|
||
<td id="76DF6144FFF5003FBF85914DCE70FD07" box="[684,798,633,655]" gridcol="1" gridrow="3" pageId="11" pageNumber="110">2.2</td>
|
||
<td id="76DF6144FFF5003FBE98914DC94CFD07" box="[945,1058,633,655]" gridcol="2" gridrow="3" pageId="11" pageNumber="110">1</td>
|
||
<td id="76DF6144FFF5003FB982914DC875FD07" box="[1195,1307,633,655]" gridcol="3" gridrow="3" pageId="11" pageNumber="110">1</td>
|
||
</tr>
|
||
<tr id="350E0838FFF5003FBDB69196C875FD30" box="[159,1307,674,696]" gridrow="4" pageId="11" pageNumber="110">
|
||
<th id="76DF6144FFF5003FBDB69196CCC7FD30" box="[159,425,674,696]" gridcol="0" gridrow="4" pageId="11" pageNumber="110">LPV (intrabulbar portion)</th>
|
||
<td id="76DF6144FFF5003FBF859196CE70FD30" box="[684,798,674,696]" gridcol="1" gridrow="4" pageId="11" pageNumber="110">0.7</td>
|
||
<td id="76DF6144FFF5003FBE989196C94CFD30" box="[945,1058,674,696]" gridcol="2" gridrow="4" pageId="11" pageNumber="110">0.7</td>
|
||
<td id="76DF6144FFF5003FB9829196C875FD30" box="[1195,1307,674,696]" gridcol="3" gridrow="4" pageId="11" pageNumber="110">0.4</td>
|
||
</tr>
|
||
<tr id="350E0838FFF5003FBDB691F8C875FD6A" box="[159,1307,716,738]" gridrow="5" pageId="11" pageNumber="110">
|
||
<th id="76DF6144FFF5003FBDB691F8CCC7FD6A" box="[159,425,716,738]" gridcol="0" gridrow="5" pageId="11" pageNumber="110">DPVP</th>
|
||
<td id="76DF6144FFF5003FBF8591F8CE70FD6A" box="[684,798,716,738]" gridcol="1" gridrow="5" pageId="11" pageNumber="110">0.5</td>
|
||
<td id="76DF6144FFF5003FBE9891F8C94CFD6A" box="[945,1058,716,738]" gridcol="2" gridrow="5" pageId="11" pageNumber="110">1.7</td>
|
||
<td id="76DF6144FFF5003FB98291F8C875FD6A" box="[1195,1307,716,738]" gridcol="3" gridrow="5" pageId="11" pageNumber="110">0.7</td>
|
||
</tr>
|
||
<tr id="350E0838FFF5003FBDB691C1C875FC83" box="[159,1307,757,779]" gridrow="6" pageId="11" pageNumber="110">
|
||
<th id="76DF6144FFF5003FBDB691C1CCC7FC83" box="[159,425,757,779]" gridcol="0" gridrow="6" pageId="11" pageNumber="110">LMA</th>
|
||
<td id="76DF6144FFF5003FBF8591C1CE70FC83" box="[684,798,757,779]" gridcol="1" gridrow="6" pageId="11" pageNumber="110">2.5</td>
|
||
<td id="76DF6144FFF5003FBE9891C1C94CFC83" box="[945,1058,757,779]" gridcol="2" gridrow="6" pageId="11" pageNumber="110">0.8</td>
|
||
<td id="76DF6144FFF5003FB98291C1C875FC83" box="[1195,1307,757,779]" gridcol="3" gridrow="6" pageId="11" pageNumber="110">0.75</td>
|
||
</tr>
|
||
<tr id="350E0838FFF5003FBDB6902AC875FCBC" box="[159,1307,798,820]" gridrow="7" pageId="11" pageNumber="110">
|
||
<th id="76DF6144FFF5003FBDB6902ACCC7FCBC" box="[159,425,798,820]" gridcol="0" gridrow="7" pageId="11" pageNumber="110">Location of ovaries</th>
|
||
<td id="76DF6144FFF5003FBF85902ACE70FCBC" box="[684,798,798,820]" gridcol="1" gridrow="7" pageId="11" pageNumber="110">12.7 (14%)</td>
|
||
<td id="76DF6144FFF5003FBE98902AC94CFCBC" box="[945,1058,798,820]" gridcol="2" gridrow="7" pageId="11" pageNumber="110">9.4 (17%)</td>
|
||
<td id="76DF6144FFF5003FB982902AC875FCBC" box="[1195,1307,798,820]" gridcol="3" gridrow="7" pageId="11" pageNumber="110">10.8 (30%)</td>
|
||
</tr>
|
||
<tr id="350E0838FFF5003FBDB6907CC875FCD6" box="[159,1307,840,862]" gridrow="8" pageId="11" pageNumber="110">
|
||
<th id="76DF6144FFF5003FBDB6907CCCC7FCD6" box="[159,425,840,862]" gridcol="0" gridrow="8" pageId="11" pageNumber="110">LCOD</th>
|
||
<td id="76DF6144FFF5003FBF85907CCE70FCD6" box="[684,798,840,862]" gridcol="1" gridrow="8" pageId="11" pageNumber="110">0.8</td>
|
||
<td id="76DF6144FFF5003FBE98907CC94CFCD6" box="[945,1058,840,862]" gridcol="2" gridrow="8" pageId="11" pageNumber="110">0.25</td>
|
||
<td id="76DF6144FFF5003FB982907CC875FCD6" box="[1195,1307,840,862]" gridcol="3" gridrow="8" pageId="11" pageNumber="110">0.55</td>
|
||
</tr>
|
||
<tr id="350E0838FFF5003FBDB69045C875FC0F" box="[159,1307,881,903]" gridrow="9" pageId="11" pageNumber="110">
|
||
<th id="76DF6144FFF5003FBDB69045CCC7FC0F" box="[159,425,881,903]" gridcol="0" gridrow="9" pageId="11" pageNumber="110">LFC</th>
|
||
<td id="76DF6144FFF5003FBF859045CE70FC0F" box="[684,798,881,903]" gridcol="1" gridrow="9" pageId="11" pageNumber="110">0.05</td>
|
||
<td id="76DF6144FFF5003FBE989045C94CFC0F" box="[945,1058,881,903]" gridcol="2" gridrow="9" pageId="11" pageNumber="110">0.05</td>
|
||
<td id="76DF6144FFF5003FB9829045C875FC0F" box="[1195,1307,881,903]" gridcol="3" gridrow="9" pageId="11" pageNumber="110">0.05</td>
|
||
</tr>
|
||
<tr id="350E0838FFF5003FBDB690AEC875FC38" box="[159,1307,922,944]" gridrow="10" pageId="11" pageNumber="110">
|
||
<th id="76DF6144FFF5003FBDB690AECCC7FC38" box="[159,425,922,944]" gridcol="0" gridrow="10" pageId="11" pageNumber="110">LFA</th>
|
||
<td id="76DF6144FFF5003FBF8590AECE70FC38" box="[684,798,922,944]" gridcol="1" gridrow="10" pageId="11" pageNumber="110">1.6</td>
|
||
<td id="76DF6144FFF5003FBE9890AEC94CFC38" box="[945,1058,922,944]" gridcol="2" gridrow="10" pageId="11" pageNumber="110">0.5</td>
|
||
<td id="76DF6144FFF5003FB98290AEC875FC38" box="[1195,1307,922,944]" gridcol="3" gridrow="10" pageId="11" pageNumber="110">0.75</td>
|
||
</tr>
|
||
</table>
|
||
</paragraph>
|
||
<paragraph id="8B810A7AFFF5FFCBBDEE90C1CFC8FAFE" blockId="11.[151,1437,1013,2010]" pageId="11" pageNumber="110">
|
||
Ovaries (150–220µm long and 130–210µm diameter) almost spherical in shape, located immediately beneath the intestine branches and among muscle fibres of the sub-intestinal parenchymal layer (
|
||
<figureCitation id="130516FFFFF5FFCBB9A2972DC86BFBBA" box="[1163,1285,1049,1074]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="11" pageNumber="110">Fig. 6F, G</figureCitation>
|
||
). They are at
|
||
<quantity id="4CC6A79FFFF5FFCBBDBE9709CC4BFBDE" box="[151,293,1085,1110]" metricMagnitude="-2" metricUnit="m" metricValue="1.1099999999999999" metricValueMax="1.27" metricValueMin="0.95" pageId="11" pageNumber="110" unit="mm" value="11.1" valueMax="12.7" valueMin="9.5">9.5–12.7mm</quantity>
|
||
from the anterior end (14–30% of body length) (
|
||
<tableCitation id="C6BC3FC1FFF5FFCBBE679709CEC8FBDE" box="[846,934,1085,1110]" captionStart="TABLE 2" captionStartId="11.[151,239,362,385]" captionText="TABLE 2. Measurements (mm) of reproductive organs of Imbira negrita sp. nov. DPVP, distance between prostatic vesicle and pharyngeal pouch; LCOD, length of common glandular ovovitelline duct; LFA, length of female atrium; LFC, length of female canal; LMA, length of male atrium; LPP, length of penis papilla; LPV, length of prostatic vesicle. The numbers given in parentheses represent the position relative to body length (%)." pageId="11" pageNumber="110">Table 2</tableCitation>
|
||
). Ovovitelline ducts emerge from the lateroventral face of ovaries (
|
||
<figureCitation id="130516FFFFF5FFCBBC8D9755CC95FBF2" box="[420,507,1121,1146]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="11" pageNumber="110">Fig. 6F</figureCitation>
|
||
). In the pre-pharyngeal region, they are located between the sub-intestinal muscle fibres and nervous plate (
|
||
<figureCitation id="130516FFFFF5FFCBBC9D97B1CF43FB16" box="[436,557,1157,1182]" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="11" pageNumber="110">Fig. 4C, E</figureCitation>
|
||
). Before the gonopore, ovovitelline ducts ascend in their course to the median plane of the body. At the level of the gonopore, they join each other in a long common glandular ovovitelline duct (250–800µm long). This duct runs dorsally to the female atrium with a convex course and curves distally to open into the postero-dorsal region of the female atrium through a very short female canal (~50µm in length) (
|
||
<figureCitation id="130516FFFFF5FFCBB80797C5CDC0FAA5" captionStart="FIGURE 5" captionStartId="10.[151,249,1686,1708]" captionTargetBox="[254,1337,197,1646]" captionTargetId="figure@10.[246,1340,193,1664]" captionTargetPageId="10" captionText="FIGURE 5. Imbira negrita sp. nov. (holotype). (A) Sagittal section of the pharynx. (B–D) Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm." httpUri="https://zenodo.org/record/1076330/files/figure.png" pageId="11" pageNumber="110">Figs. 5B– D</figureCitation>
|
||
,
|
||
<figureCitation id="130516FFFFF5FFCBBD929621CD8FFAA6" box="[187,225,1301,1326]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="11" pageNumber="110">6H</figureCitation>
|
||
). Female atrium (
|
||
<quantity id="4CC6A79FFFF5FFCBBC859621CF43FAA6" box="[428,557,1301,1326]" metricMagnitude="-3" metricUnit="m" metricValue="1.05" metricValueMax="1.6" metricValueMin="0.5" pageId="11" pageNumber="110" unit="mm" value="1.05" valueMax="1.6" valueMin="0.5">0.5–1.6mm</quantity>
|
||
in length) without folded walls, but with a narrow lumen because it is lined with a tall epithelium (see below) (
|
||
<figureCitation id="130516FFFFF5FFCBBF0F960DCFC3FADA" box="[550,685,1337,1362]" captionStart="FIGURE 5" captionStartId="10.[151,249,1686,1708]" captionTargetBox="[254,1337,197,1646]" captionTargetId="figure@10.[246,1340,193,1664]" captionTargetPageId="10" captionText="FIGURE 5. Imbira negrita sp. nov. (holotype). (A) Sagittal section of the pharynx. (B–D) Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm." httpUri="https://zenodo.org/record/1076330/files/figure.png" pageId="11" pageNumber="110">Figs. 5B–D</figureCitation>
|
||
,
|
||
<figureCitation id="130516FFFFF5FFCBBF90960DCE4EFADA" box="[697,800,1337,1362]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="11" pageNumber="110">6C–E, H</figureCitation>
|
||
). Vitellaria, well developed (except
|
||
<typeStatus id="5485B4D8FFF5FFCBB995960EC872FADA" box="[1212,1308,1338,1362]" pageId="11" pageNumber="110" type="paratype">paratype</typeStatus>
|
||
1), located among intestinal branches (
|
||
<figureCitation id="130516FFFFF5FFCBBCE29669CF22FAFE" box="[459,588,1373,1398]" captionStart="FIGURE 4" captionStartId="9.[151,249,1834,1856]" captionTargetBox="[249,1350,342,1810]" captionTargetId="figure@9.[234,1353,338,1812]" captionTargetPageId="9" captionText="FIGURE 4. Imbira negrita sp. nov. (A) Photograph of a live specimen (holotype), in dorsal view. In the inset, the same specimen showing part of the ventral surface. (B) Half of a transverse section at the cephalic region (on the left) (paratype 1) and near the ovaries (on the right) (holotype). Arrowheads indicate longitudinal parenchymal muscle fibres. C Transverse section of the pre-pharyngeal region. (D, E) Transverse sections of the pre-pharyngeal region with details: dorsal to intestine (D), ventral to intestine (E). Scale bars:A: 10mm, B, D, E: 250µm, C: 500µm." httpUri="https://zenodo.org/record/1076328/files/figure.png" pageId="11" pageNumber="110">Figs. 4C–E</figureCitation>
|
||
,
|
||
<figureCitation id="130516FFFFF5FFCBBF7E9669CFF7FAFE" box="[599,665,1373,1398]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="11" pageNumber="110">6F, G</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8B810A7AFFF5FFCBBDEE96B5CCCDF932" blockId="11.[151,1437,1013,2010]" pageId="11" pageNumber="110">
|
||
Lining epithelium of the ovovitelline ducts ciliated and cuboidal, wrapped by a thin circular muscle layer (5µm thick). Common glandular ovovitelline duct lined with cuboidal to columnar ciliated epithelium, followed by a muscle layer with circular fibres and some oblique and longitudinal fibres (10–15µm thick). Similarly to the ascending portions of the ovovitelline ducts, it receives erythrophil secretion from shell glands (
|
||
<figureCitation id="130516FFFFF5FFCBB9EF96D9C824F98E" box="[1222,1354,1517,1542]" captionStart="FIGURE 5" captionStartId="10.[151,249,1686,1708]" captionTargetBox="[254,1337,197,1646]" captionTargetId="figure@10.[246,1340,193,1664]" captionTargetPageId="10" captionText="FIGURE 5. Imbira negrita sp. nov. (holotype). (A) Sagittal section of the pharynx. (B–D) Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm." httpUri="https://zenodo.org/record/1076330/files/figure.png" pageId="11" pageNumber="110">Figs. 5B–D</figureCitation>
|
||
,
|
||
<figureCitation id="130516FFFFF5FFCBB87C96D9CDC1F9A2" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="11" pageNumber="110">6C–E, H</figureCitation>
|
||
). Female canal lined with ciliated and columnar epithelium, pierced by fine erythrophil granules, followed by circular muscle fibres (5–10µm thick). Lining epithelium of female atrium with stratified appearance, except near the gonopore, where it is columnar (
|
||
<figureCitation id="130516FFFFF5FFCBBF74956DCF84F9FA" box="[605,746,1625,1650]" captionStart="FIGURE 5" captionStartId="10.[151,249,1686,1708]" captionTargetBox="[254,1337,197,1646]" captionTargetId="figure@10.[246,1340,193,1664]" captionTargetPageId="10" captionText="FIGURE 5. Imbira negrita sp. nov. (holotype). (A) Sagittal section of the pharynx. (B–D) Schematic reconstructions of the copulatory apparatus in sagittal view: holotype (B), paratype 1 (C), paratype 2 (D). Scale bars: A: 1mm, B–D: 500µm." httpUri="https://zenodo.org/record/1076330/files/figure.png" pageId="11" pageNumber="110">Figs. 5B–D</figureCitation>
|
||
,
|
||
<figureCitation id="130516FFFFF5FFCBBFD7956DCE05F9FA" box="[766,875,1625,1650]" captionStart="FIGURE 6" captionStartId="12.[151,249,1434,1456]" captionTargetBox="[254,1331,199,1400]" captionTargetId="figure@12.[248,1338,193,1412]" captionTargetPageId="12" captionText="FIGURE 6. Imbira negrita sp. nov. Sagittal sections of the reproductive system. (A) Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype). (B) Detail of the male reproductive system (holotype). (C) Copulatory apparatus of the holotype. (D) Copulatory apparatus of paratype 1. (E) Copulatory apparatus of paratype 2. (F) Latero-external portion of the ovary (holotype). (G) Middle portion of the ovary (arrow indicates nematode larva) (holotype). (H) Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles.Scale bars: A:250µm, B–E: 500µm,F, G: 200µm, H:100µm." httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="11" pageNumber="110">6C–E, H</figureCitation>
|
||
). Abundant fine granular erythrophil secretion discharges into the atrial epithelium. Muscularis of female atrium composed of circular, oblique and longitudinal fibres (25–35µm thick).
|
||
</paragraph>
|
||
<paragraph id="8B810A7AFFF5FFCBBDEE95F1C9EEF852" blockId="11.[151,1437,1013,2010]" pageId="11" pageNumber="110">
|
||
<emphasis id="B94AD668FFF5FFCBBDEE95F1CC85F956" bold="true" box="[199,491,1733,1758]" pageId="11" pageNumber="110">Comparative discussion.</emphasis>
|
||
The new species here described fits well with the diagnosis of
|
||
<taxonomicName id="4C3E71F9FFF5FFCBB98F95F1CDBDF88A" authority="Carbayo et al., 2013" authorityName="Carbayo et al." authorityYear="2013" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="11" pageNumber="110" phylum="Platyhelminthes" rank="genus">
|
||
<emphasis id="B94AD668FFF5FFCBB98F95F1C99DF955" box="[1190,1267,1733,1757]" italics="true" pageId="11" pageNumber="110">Imbira</emphasis>
|
||
<bibRefCitation id="EFAF778BFFF5FFCBB9D195F1CDBDF88A" author="Carbayo" pageId="11" pageNumber="110" refString="Carbayo, F., Alvarez-Presas, M., Olivares, M. T., Marques, F. P. L., Froehlich, E. M. & Riutort, M. (2013) Molecular phylogeny of Geoplaninae (Platyhelminthes) challenges current classification: proposal of taxonomic actions. Zoologica Scripta, 42, 508 - 528. https: // doi. org / 10.1111 / zsc. 12019" type="journal article" year="2013">
|
||
Carbayo
|
||
<emphasis id="B94AD668FFF5FFCBB87495F2C8FBF955" box="[1373,1429,1733,1757]" italics="true" pageId="11" pageNumber="110">et al.</emphasis>
|
||
, 2013
|
||
</bibRefCitation>
|
||
</taxonomicName>
|
||
. This genus, which includes only two species, both recorded in the Brazilian Atlantic Forest, characterizes geoplaninid flatworms with elongated (around
|
||
<quantity id="4CC6A79FFFF5FFCBBFEB943ACE0DF8AE" box="[706,867,1806,1830]" metricMagnitude="-1" metricUnit="m" metricValue="1.45" metricValueMax="2.0" metricValueMin="0.9" pageId="11" pageNumber="110" unit="mm" value="145.0" valueMax="200.0" valueMin="90.0">90 to 200mm</quantity>
|
||
in length, in maximum extension), slender and flattened body, and with parallel margins; eyes marginal along the body; and presence of longitudinal parenchymal muscle fibres. In addition, the reproductive system shows a tubular prostatic vesicle with paired proximal portions; male atrium folded with eversible penis; ovovitelline ducts ascending laterally to gonopore and joining each other dorsally to female atrium; short female canal dorso-anteriorly flexed; and lining epithelium of female atrium with multi-layered aspect (
|
||
<bibRefCitation id="EFAF778BFFF5FFCBBCA094F6CF4FF852" author="Marcus" box="[393,545,1985,2010]" pageId="11" pageNumber="110" refString="Marcus, E. (1951) Turbellaria brasileiros (9). Boletins da Faculdade de Filosofia, Ciencias e Letras da Universidade Sao Paulo, Serie Zoologia, 16, 5 - 215. https: // doi. org / 10.11606 / issn. 2526 - 4877. bsffclzoologia. 1951.125221" type="journal article" year="1951">Marcus 1951</bibRefCitation>
|
||
;
|
||
<bibRefCitation id="EFAF778BFFF5FFCBBF0494F5CEE9F852" author="Leal-Zanchet" box="[557,903,1985,2010]" pageId="11" pageNumber="110" refString="Leal-Zanchet, A. M. & Carbayo, F. (2001) Two new species of land planarians (Platyhelminthes, Tricladida, Terricola) of South Brazil. Journal of Zoology, 253, 433 - 446. https: // doi. org / 10.1017 / S 0952836901000401" type="journal article" year="2001">Leal-Zanchet & Carbayo 2001</bibRefCitation>
|
||
;
|
||
<bibRefCitation id="EFAF778BFFF5FFCBBEBB94F5C91AF852" author="Carbayo" box="[914,1140,1985,2010]" pageId="11" pageNumber="110" refString="Carbayo, F., Alvarez-Presas, M., Olivares, M. T., Marques, F. P. L., Froehlich, E. M. & Riutort, M. (2013) Molecular phylogeny of Geoplaninae (Platyhelminthes) challenges current classification: proposal of taxonomic actions. Zoologica Scripta, 42, 508 - 528. https: // doi. org / 10.1111 / zsc. 12019" type="journal article" year="2013">
|
||
Carbayo
|
||
<emphasis id="B94AD668FFF5FFCBBED194F6C95FF851" box="[1016,1073,1985,2009]" italics="true" pageId="11" pageNumber="110">et al.</emphasis>
|
||
2013
|
||
</bibRefCitation>
|
||
).
|
||
</paragraph>
|
||
<caption id="DF415AF2FFF2FFCCBDBE96AECE09F9C1" httpUri="https://zenodo.org/record/1076334/files/figure.png" pageId="12" pageNumber="111" targetBox="[254,1331,199,1400]" targetPageId="12">
|
||
<paragraph id="8B810A7AFFF2FFCCBDBE96AECE09F9C1" blockId="12.[151,1437,1433,1609]" pageId="12" pageNumber="111">
|
||
<emphasis id="B94AD668FFF2FFCCBDBE96AECC7BFA27" bold="true" box="[151,277,1434,1456]" pageId="12" pageNumber="111">FIGURE 6.</emphasis>
|
||
<taxonomicName id="4C3E71F9FFF2FFCCBC3796AECCDBFA38" authority="Negrete & Brusa, 2017" authorityName="Negrete & Brusa" authorityYear="2017" box="[286,437,1434,1456]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="12" pageNumber="111" phylum="Platyhelminthes" rank="species" species="negrita" status="sp. nov.">
|
||
<emphasis id="B94AD668FFF2FFCCBC3796AECCDBFA38" box="[286,437,1434,1456]" italics="true" pageId="12" pageNumber="111">Imbira negrita</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A2796B13FFF2FFCCBC9796AECF7FFA27" box="[446,529,1434,1455]" pageId="12" pageNumber="111" rank="species">
|
||
<emphasis id="B94AD668FFF2FFCCBC9796AECF7FFA27" bold="true" box="[446,529,1434,1455]" pageId="12" pageNumber="111">sp. nov.</emphasis>
|
||
</taxonomicNameLabel>
|
||
Sagittal sections of the reproductive system.
|
||
<emphasis id="B94AD668FFF2FFCCBEC696ADC97DFA38" bold="true" box="[1007,1043,1433,1456]" pageId="12" pageNumber="111">(A)</emphasis>
|
||
Detail of the proximal portion of the prostatic vesicle (arrows indicate prostatic secretion) (holotype).
|
||
<emphasis id="B94AD668FFF2FFCCBE10968CCE35FA47" bold="true" box="[825,859,1464,1487]" pageId="12" pageNumber="111">(B)</emphasis>
|
||
Detail of the male reproductive system (holotype).
|
||
<emphasis id="B94AD668FFF2FFCCB850968CC8F2FA47" bold="true" box="[1401,1436,1464,1487]" pageId="12" pageNumber="111">(C)</emphasis>
|
||
Copulatory apparatus of the holotype.
|
||
<emphasis id="B94AD668FFF2FFCCBF0096E2CF22FA65" bold="true" box="[553,588,1494,1517]" pageId="12" pageNumber="111">(D)</emphasis>
|
||
Copulatory apparatus of paratype 1.
|
||
<emphasis id="B94AD668FFF2FFCCBEFA96E2CE9BFA65" bold="true" box="[979,1013,1494,1517]" pageId="12" pageNumber="111">(E)</emphasis>
|
||
Copulatory apparatus of paratype 2.
|
||
<emphasis id="B94AD668FFF2FFCCB85596E2C8F2FA65" bold="true" box="[1404,1436,1494,1517]" pageId="12" pageNumber="111">(F)</emphasis>
|
||
Latero-external portion of the ovary (holotype).
|
||
<emphasis id="B94AD668FFF2FFCCBFAD96C1CFC4F984" bold="true" box="[644,682,1525,1548]" pageId="12" pageNumber="111">(G)</emphasis>
|
||
Middle portion of the ovary (arrow indicates nematode larva) (holotype).
|
||
<emphasis id="B94AD668FFF2FFCCBDBE9520CDD2F9A3" bold="true" box="[151,188,1556,1579]" pageId="12" pageNumber="111">(H)</emphasis>
|
||
Detail of the female atrium (holotype). In F and G, arrowheads indicate longitudinal parenchymal muscle fibres in discrete bundles. Scale bars: A: 250µm, B–E: 500µm, F, G: 200µm, H: 100µm.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8B810A7AFFF2FFCDBDEE9544CF30FE7D" blockId="12.[151,1437,1648,2033]" lastBlockId="13.[151,1437,151,896]" lastPageId="13" lastPageNumber="112" pageId="12" pageNumber="111">
|
||
Externally,
|
||
<taxonomicName id="4C3E71F9FFF2FFCCBC609544CC80F900" authority="Negrete & Brusa, 2017" authorityName="Negrete & Brusa" authorityYear="2017" box="[329,494,1648,1672]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="12" pageNumber="111" phylum="Platyhelminthes" rank="species" species="negrita" status="sp. nov.">
|
||
<emphasis id="B94AD668FFF2FFCCBC609544CC80F900" box="[329,494,1648,1672]" italics="true" pageId="12" pageNumber="111">Imbira negrita</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A2796B13FFF2FFCCBCDF9544CF3FF901" box="[502,593,1648,1673]" pageId="12" pageNumber="111" rank="species">
|
||
<emphasis id="B94AD668FFF2FFCCBCDF9544CF3FF901" bold="true" box="[502,593,1648,1673]" pageId="12" pageNumber="111">sp. nov.</emphasis>
|
||
</taxonomicNameLabel>
|
||
, whose dorsal pigmentation is homogeneous black, is easily distinguished from the other species of the genus: in
|
||
<taxonomicName id="4C3E71F9FFF2FFCCBF7D95A1CE9CF925" authority="Carbayo et al., 2013" authorityName="Carbayo et al." authorityYear="2013" box="[596,1010,1684,1709]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="12" pageNumber="111" phylum="Platyhelminthes" rank="species" species="marcusi">
|
||
<emphasis id="B94AD668FFF2FFCCBF7D95A1CE6CF924" box="[596,770,1685,1709]" italics="true" pageId="12" pageNumber="111">Imbira marcusi</emphasis>
|
||
<bibRefCitation id="EFAF778BFFF2FFCCBE2295A0CE9CF925" author="Carbayo" box="[779,1010,1684,1709]" pageId="12" pageNumber="111" refString="Carbayo, F., Alvarez-Presas, M., Olivares, M. T., Marques, F. P. L., Froehlich, E. M. & Riutort, M. (2013) Molecular phylogeny of Geoplaninae (Platyhelminthes) challenges current classification: proposal of taxonomic actions. Zoologica Scripta, 42, 508 - 528. https: // doi. org / 10.1111 / zsc. 12019" type="journal article" year="2013">
|
||
Carbayo
|
||
<emphasis id="B94AD668FFF2FFCCBE5B95A1CEC5F925" box="[882,939,1685,1709]" italics="true" pageId="12" pageNumber="111">et al.</emphasis>
|
||
, 2013
|
||
</bibRefCitation>
|
||
</taxonomicName>
|
||
(see
|
||
<taxonomicName id="4C3E71F9FFF2FFCCB91995A1C86AF925" authorityName="Riester" authorityYear="1938" box="[1072,1284,1685,1709]" class="Turbellaria" family="Geoplanidae" genus="Geoplana" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="12" pageNumber="111" phylum="Platyhelminthes" rank="species" species="goetschi">
|
||
<emphasis id="B94AD668FFF2FFCCB91995A1C86AF925" box="[1072,1284,1685,1709]" italics="true" pageId="12" pageNumber="111">Geoplana goetschi</emphasis>
|
||
</taxonomicName>
|
||
after
|
||
<bibRefCitation id="EFAF778BFFF2FFCCB86195A1CD83F959" author="Marcus" pageId="12" pageNumber="111" refString="Marcus, E. (1951) Turbellaria brasileiros (9). Boletins da Faculdade de Filosofia, Ciencias e Letras da Universidade Sao Paulo, Serie Zoologia, 16, 5 - 215. https: // doi. org / 10.11606 / issn. 2526 - 4877. bsffclzoologia. 1951.125221" type="journal article" year="1951">Marcus (1951))</bibRefCitation>
|
||
, the dorsal ground colour is dark green olive, sometimes brownish or black, with a sub-marginal yellowish stripe contouring the body (
|
||
<bibRefCitation id="EFAF778BFFF2FFCCBCE295E9CF0DF97D" author="Marcus" box="[459,611,1756,1781]" pageId="12" pageNumber="111" refString="Marcus, E. (1951) Turbellaria brasileiros (9). Boletins da Faculdade de Filosofia, Ciencias e Letras da Universidade Sao Paulo, Serie Zoologia, 16, 5 - 215. https: // doi. org / 10.11606 / issn. 2526 - 4877. bsffclzoologia. 1951.125221" type="journal article" year="1951">Marcus 1951</bibRefCitation>
|
||
;
|
||
<bibRefCitation id="EFAF778BFFF2FFCCBF4695E8CEF7F97D" author="Alvarez-Presas" box="[623,921,1756,1781]" pageId="12" pageNumber="111" refString="Alvarez-Presas, M., Carbayo, F., Rozas, J. & Riutort, M. (2011) Land planarians (Platyhelminthes) as a model organism for fine-scale phylogeographic studies: understanding patterns of biodiversity in the Brazilian Atlantic Forest hotspot. Journal of Evolutionary Biology, 24, 887 - 896. https: // doi. org / 10.1111 / j. 1420 - 9101.2010.02220. x" type="journal article" year="2011">
|
||
Álvarez-Presas
|
||
<emphasis id="B94AD668FFF2FFCCBE3695E9CE39F97D" box="[799,855,1757,1781]" italics="true" pageId="12" pageNumber="111">et al.</emphasis>
|
||
2011
|
||
</bibRefCitation>
|
||
), whereas in
|
||
<taxonomicName id="4C3E71F9FFF2FFCCB90295E9CC29F891" authority="(Leal-Zanchet & Carbayo, 2001)" baseAuthorityName="Leal-Zanchet & Carbayo" baseAuthorityYear="2001" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="12" pageNumber="111" phylum="Platyhelminthes" rank="species" species="guaiana">
|
||
<emphasis id="B94AD668FFF2FFCCB90295E9C9B4F97C" box="[1067,1242,1757,1781]" italics="true" pageId="12" pageNumber="111">Imbira guaiana</emphasis>
|
||
(
|
||
<bibRefCitation id="EFAF778BFFF2FFCCB9C195E8CC2EF891" author="Leal-Zanchet" pageId="12" pageNumber="111" refString="Leal-Zanchet, A. M. & Carbayo, F. (2001) Two new species of land planarians (Platyhelminthes, Tricladida, Terricola) of South Brazil. Journal of Zoology, 253, 433 - 446. https: // doi. org / 10.1017 / S 0952836901000401" type="journal article" year="2001">Leal-Zanchet & Carbayo, 2001</bibRefCitation>
|
||
)
|
||
</taxonomicName>
|
||
, the dorsal surface is greyish-olive (
|
||
<bibRefCitation id="EFAF778BFFF2FFCCBFF39434C958F891" author="Leal-Zanchet" box="[730,1078,1792,1817]" pageId="12" pageNumber="111" refString="Leal-Zanchet, A. M. & Carbayo, F. (2001) Two new species of land planarians (Platyhelminthes, Tricladida, Terricola) of South Brazil. Journal of Zoology, 253, 433 - 446. https: // doi. org / 10.1017 / S 0952836901000401" type="journal article" year="2001">Leal-Zanchet & Carbayo 2001</bibRefCitation>
|
||
). Regarding the body shape, the new species resembles
|
||
<taxonomicName id="4C3E71F9FFF2FFCCBC8C9411CF72F8B4" baseAuthorityName="Leal-Zanchet & Carbayo" baseAuthorityYear="2001" box="[421,540,1829,1852]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="12" pageNumber="111" phylum="Platyhelminthes" rank="species" species="guaiana">
|
||
<emphasis id="B94AD668FFF2FFCCBC8C9411CF72F8B4" box="[421,540,1829,1852]" italics="true" pageId="12" pageNumber="111">I. guaiana</emphasis>
|
||
</taxonomicName>
|
||
because both species look like a ribbon, whereas in
|
||
<taxonomicName id="4C3E71F9FFF2FFCCB9A09411C992F8B4" box="[1161,1276,1829,1852]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="12" pageNumber="111" phylum="Platyhelminthes" rank="species" species="marcusi">
|
||
<emphasis id="B94AD668FFF2FFCCB9A09411C992F8B4" box="[1161,1276,1829,1852]" italics="true" pageId="12" pageNumber="111">I. marcusi</emphasis>
|
||
</taxonomicName>
|
||
, which is the widest species of the genus, the body is almost lanceolate (
|
||
<bibRefCitation id="EFAF778BFFF2FFCCBE6B947DC8FFF8E9" author="Marcus" box="[834,1425,1864,1889]" pageId="12" pageNumber="111" refString="Marcus, E. (1951) Turbellaria brasileiros (9). Boletins da Faculdade de Filosofia, Ciencias e Letras da Universidade Sao Paulo, Serie Zoologia, 16, 5 - 215. https: // doi. org / 10.11606 / issn. 2526 - 4877. bsffclzoologia. 1951.125221" type="journal article" year="1951">Marcus 1951, photos in http://planarias.each.usp.br</bibRefCitation>
|
||
). The pharynx of
|
||
<taxonomicName id="4C3E71F9FFF2FFCCBC669459CCD7F80C" authority="Negrete & Brusa, 2017" authorityName="Negrete & Brusa" authorityYear="2017" box="[335,441,1901,1924]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="12" pageNumber="111" phylum="Platyhelminthes" rank="species" species="negrita" status="sp. nov.">
|
||
<emphasis id="B94AD668FFF2FFCCBC669459CCD7F80C" box="[335,441,1901,1924]" italics="true" pageId="12" pageNumber="111">I. negrita</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A2796B13FFF2FFCCBCEB9458CF73F80D" box="[450,541,1900,1925]" pageId="12" pageNumber="111" rank="species">
|
||
<emphasis id="B94AD668FFF2FFCCBCEB9458CF73F80D" bold="true" box="[450,541,1900,1925]" pageId="12" pageNumber="111">sp. nov.</emphasis>
|
||
</taxonomicNameLabel>
|
||
is similar to that of
|
||
<taxonomicName id="4C3E71F9FFF2FFCCBE209459CE10F80C" baseAuthorityName="Leal-Zanchet & Carbayo" baseAuthorityYear="2001" box="[777,894,1901,1924]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="12" pageNumber="111" phylum="Platyhelminthes" rank="species" species="guaiana">
|
||
<emphasis id="B94AD668FFF2FFCCBE209459CE10F80C" box="[777,894,1901,1924]" italics="true" pageId="12" pageNumber="111">I. guaiana</emphasis>
|
||
</taxonomicName>
|
||
, which is collar-type (
|
||
<bibRefCitation id="EFAF778BFFF2FFCCB9569458CDBDF821" author="Leal-Zanchet" pageId="12" pageNumber="111" refString="Leal-Zanchet, A. M. & Carbayo, F. (2001) Two new species of land planarians (Platyhelminthes, Tricladida, Terricola) of South Brazil. Journal of Zoology, 253, 433 - 446. https: // doi. org / 10.1017 / S 0952836901000401" type="journal article" year="2001">Leal-Zanchet & Carbayo 2001</bibRefCitation>
|
||
). In the new species, it is between bell-shaped and collar-type, although in the largest sectioned specimen the pharynx is collar-type. Although
|
||
<bibRefCitation id="EFAF778BFFF2FFCCBF3F9481CFD0F845" author="Marcus" box="[534,702,1972,1997]" pageId="12" pageNumber="111" refString="Marcus, E. (1951) Turbellaria brasileiros (9). Boletins da Faculdade de Filosofia, Ciencias e Letras da Universidade Sao Paulo, Serie Zoologia, 16, 5 - 215. https: // doi. org / 10.11606 / issn. 2526 - 4877. bsffclzoologia. 1951.125221" type="journal article" year="1951">Marcus (1951)</bibRefCitation>
|
||
stated that the pharynx in
|
||
<taxonomicName id="4C3E71F9FFF2FFCCBED09481C903F844" box="[1017,1133,1973,1996]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="12" pageNumber="111" phylum="Platyhelminthes" rank="species" species="marcusi">
|
||
<emphasis id="B94AD668FFF2FFCCBED09481C903F844" box="[1017,1133,1973,1996]" italics="true" pageId="12" pageNumber="111">I. marcusi</emphasis>
|
||
</taxonomicName>
|
||
is cylindrical, with dorsal insertion at the level of the mouth (
|
||
<bibRefCitation id="EFAF778BFFF2FFCCBF0B94EDCE60F879" author="Marcus" box="[546,782,2008,2033]" pageId="12" pageNumber="111" refString="Marcus, E. (1951) Turbellaria brasileiros (9). Boletins da Faculdade de Filosofia, Ciencias e Letras da Universidade Sao Paulo, Serie Zoologia, 16, 5 - 215. https: // doi. org / 10.11606 / issn. 2526 - 4877. bsffclzoologia. 1951.125221" type="journal article" year="1951" yearSuffix="p">Marcus 1951, p. 191</bibRefCitation>
|
||
), according to
|
||
<bibRefCitation id="EFAF778BFFF2FFCCBE9F94ECC99CF879" box="[950,1266,2008,2033]" pageId="12" pageNumber="111" refString="Alvarez-Presas, M., Carbayo, F., Rozas, J. & Riutort, M. (2011) Land planarians (Platyhelminthes) as a model organism for fine-scale phylogeographic studies: understanding patterns of biodiversity in the Brazilian Atlantic Forest hotspot. Journal of Evolutionary Biology, 24, 887 - 896. https: // doi. org / 10.1111 / j. 1420 - 9101.2010.02220. x" type="journal article">
|
||
Álvarez-Presas
|
||
<emphasis id="B94AD668FFF2FFCCB94F94EDC9F1F878" box="[1126,1183,2008,2032]" italics="true" pageId="12" pageNumber="111">et al.</emphasis>
|
||
(2011)
|
||
</bibRefCitation>
|
||
, who reviewed Marcus material, the pharynx is bell-shaped. The copulatory apparatus of the three species is quite similar, differing in some details. The prostatic vesicle of
|
||
<taxonomicName id="4C3E71F9FFF3FFCDBF469389CFB4FF5C" authority="Negrete & Brusa, 2017" authorityName="Negrete & Brusa" authorityYear="2017" box="[623,730,189,212]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="13" pageNumber="112" phylum="Platyhelminthes" rank="species" species="negrita" status="sp. nov.">
|
||
<emphasis id="B94AD668FFF3FFCDBF469389CFB4FF5C" box="[623,730,189,212]" italics="true" pageId="13" pageNumber="112">I. negrita</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A2796B13FFF3FFCDBFCD9388CE2FFF5D" box="[740,833,188,213]" pageId="13" pageNumber="112" rank="species">
|
||
<emphasis id="B94AD668FFF3FFCDBFCD9388CE2FFF5D" bold="true" box="[740,833,188,213]" pageId="13" pageNumber="112">sp. nov.</emphasis>
|
||
</taxonomicNameLabel>
|
||
and
|
||
<taxonomicName id="4C3E71F9FFF3FFCDBE549389CE9DFF5C" baseAuthorityName="Leal-Zanchet & Carbayo" baseAuthorityYear="2001" box="[893,1011,189,212]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="13" pageNumber="112" phylum="Platyhelminthes" rank="species" species="guaiana">
|
||
<emphasis id="B94AD668FFF3FFCDBE549389CE9DFF5C" box="[893,1011,189,212]" italics="true" pageId="13" pageNumber="112">I. guaiana</emphasis>
|
||
</taxonomicName>
|
||
exhibits two portions. Proximally, it consists of two long tubular extrabulbar branches which run backward parallel to each other, and distally they unite into an unpaired and tubular intrabulbar portion. This unpaired portion runs sinuously and opens into a short ejaculatory duct whose lumen is narrow. In
|
||
<taxonomicName id="4C3E71F9FFF3FFCDBFA3921DCF92FEB7" box="[650,764,296,320]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="13" pageNumber="112" phylum="Platyhelminthes" rank="species" species="marcusi">
|
||
<emphasis id="B94AD668FFF3FFCDBFA3921DCF92FEB7" box="[650,764,296,320]" italics="true" pageId="13" pageNumber="112">I. marcusi</emphasis>
|
||
</taxonomicName>
|
||
, the proximal part is also bifurcated and extrabulbar, but it opens directly into the ejaculatory duct, which runs along the penis bulb and traverses the eversible penis (
|
||
<bibRefCitation id="EFAF778BFFF3FFCDB86E9278CDBCFE00" author="Marcus" pageId="13" pageNumber="112" refString="Marcus, E. (1951) Turbellaria brasileiros (9). Boletins da Faculdade de Filosofia, Ciencias e Letras da Universidade Sao Paulo, Serie Zoologia, 16, 5 - 215. https: // doi. org / 10.11606 / issn. 2526 - 4877. bsffclzoologia. 1951.125221" type="journal article" year="1951">Marcus 1951</bibRefCitation>
|
||
;
|
||
<bibRefCitation id="EFAF778BFFF3FFCDBDCA925BCF72FE00" author="Alvarez-Presas" box="[227,540,367,392]" pageId="13" pageNumber="112" refString="Alvarez-Presas, M., Carbayo, F., Rozas, J. & Riutort, M. (2011) Land planarians (Platyhelminthes) as a model organism for fine-scale phylogeographic studies: understanding patterns of biodiversity in the Brazilian Atlantic Forest hotspot. Journal of Evolutionary Biology, 24, 887 - 896. https: // doi. org / 10.1111 / j. 1420 - 9101.2010.02220. x" type="journal article" year="2011">
|
||
Álvarez-Presas
|
||
<emphasis id="B94AD668FFF3FFCDBCB19245CCBBFE00" box="[408,469,368,392]" italics="true" pageId="13" pageNumber="112">et al.</emphasis>
|
||
2011
|
||
</bibRefCitation>
|
||
). It would be interesting to corroborate whether this long part identified by
|
||
<bibRefCitation id="EFAF778BFFF3FFCDBDBE92A1CC52FE25" author="Marcus" box="[151,316,404,429]" pageId="13" pageNumber="112" refString="Marcus, E. (1951) Turbellaria brasileiros (9). Boletins da Faculdade de Filosofia, Ciencias e Letras da Universidade Sao Paulo, Serie Zoologia, 16, 5 - 215. https: // doi. org / 10.11606 / issn. 2526 - 4877. bsffclzoologia. 1951.125221" type="journal article" year="1951">Marcus (1951)</bibRefCitation>
|
||
and
|
||
<bibRefCitation id="EFAF778BFFF3FFCDBC5B92A0CFC4FE24" box="[370,682,404,429]" pageId="13" pageNumber="112" refString="Alvarez-Presas, M., Carbayo, F., Rozas, J. & Riutort, M. (2011) Land planarians (Platyhelminthes) as a model organism for fine-scale phylogeographic studies: understanding patterns of biodiversity in the Brazilian Atlantic Forest hotspot. Journal of Evolutionary Biology, 24, 887 - 896. https: // doi. org / 10.1111 / j. 1420 - 9101.2010.02220. x" type="journal article">
|
||
Álvarez-Presas
|
||
<emphasis id="B94AD668FFF3FFCDBF0B92A1CF34FE24" box="[546,602,404,428]" italics="true" pageId="13" pageNumber="112">et al.</emphasis>
|
||
(2011)
|
||
</bibRefCitation>
|
||
as the ejaculatory duct is in fact the intrabulbar part of the prostatic vesicle. Similarly to that observed in
|
||
<taxonomicName id="4C3E71F9FFF3FFCDBF1F928DCFF0FE47" authority="Negrete & Brusa, 2017" authorityName="Negrete & Brusa" authorityYear="2017" box="[566,670,440,464]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="13" pageNumber="112" phylum="Platyhelminthes" rank="species" species="negrita" status="sp. nov.">
|
||
<emphasis id="B94AD668FFF3FFCDBF1F928DCFF0FE47" box="[566,670,440,464]" italics="true" pageId="13" pageNumber="112">I. negrita</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A2796B13FFF3FFCDBF8C9283CF90FE58" box="[677,766,439,464]" pageId="13" pageNumber="112" rank="species">
|
||
<emphasis id="B94AD668FFF3FFCDBF8C9283CF90FE58" bold="true" box="[677,766,439,464]" pageId="13" pageNumber="112">sp. nov.</emphasis>
|
||
</taxonomicNameLabel>
|
||
, the eversible penis and the male atrium of
|
||
<taxonomicName id="4C3E71F9FFF3FFCDB9CC928DC83BFE47" box="[1253,1365,440,464]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="13" pageNumber="112" phylum="Platyhelminthes" rank="species" species="marcusi">
|
||
<emphasis id="B94AD668FFF3FFCDB9CC928DC83BFE47" box="[1253,1365,440,464]" italics="true" pageId="13" pageNumber="112">I. marcusi</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="4C3E71F9FFF3FFCDB8A5928DCD9DFE7C" baseAuthorityName="Leal-Zanchet & Carbayo" baseAuthorityYear="2001" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="13" pageNumber="112" phylum="Platyhelminthes" rank="species" species="guaiana">
|
||
<emphasis id="B94AD668FFF3FFCDB8A5928DCD9DFE7C" italics="true" pageId="13" pageNumber="112">I. guaiana</emphasis>
|
||
</taxonomicName>
|
||
are lined by ciliated epithelium.
|
||
</paragraph>
|
||
<paragraph id="8B810A7AFFF3FFCDBDEE92CBC8E5FC08" blockId="13.[151,1437,151,896]" pageId="13" pageNumber="112">
|
||
Regarding the female reproductive system, the new species can be distinguished from
|
||
<taxonomicName id="4C3E71F9FFF3FFCDB9FD9135C824FD9F" box="[1236,1354,512,536]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="13" pageNumber="112" phylum="Platyhelminthes" rank="species" species="marcusi">
|
||
<emphasis id="B94AD668FFF3FFCDB9FD9135C824FD9F" box="[1236,1354,512,536]" italics="true" pageId="13" pageNumber="112">I. marcusi</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="4C3E71F9FFF3FFCDB8A59135CD9DFDB4" baseAuthorityName="Leal-Zanchet & Carbayo" baseAuthorityYear="2001" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="13" pageNumber="112" phylum="Platyhelminthes" rank="species" species="guaiana">
|
||
<emphasis id="B94AD668FFF3FFCDB8A59135CD9DFDB4" italics="true" pageId="13" pageNumber="112">I. guaiana</emphasis>
|
||
</taxonomicName>
|
||
because, in these species, the ovovitelline ducts emerge from the dorsal region of the ovaries (
|
||
<bibRefCitation id="EFAF778BFFF3FFCDB86E9110CDBCFDE8" author="Marcus" pageId="13" pageNumber="112" refString="Marcus, E. (1951) Turbellaria brasileiros (9). Boletins da Faculdade de Filosofia, Ciencias e Letras da Universidade Sao Paulo, Serie Zoologia, 16, 5 - 215. https: // doi. org / 10.11606 / issn. 2526 - 4877. bsffclzoologia. 1951.125221" type="journal article" year="1951">Marcus 1951</bibRefCitation>
|
||
;
|
||
<bibRefCitation id="EFAF778BFFF3FFCDBDCA9173CF22FDE8" author="Leal-Zanchet" box="[227,588,583,608]" pageId="13" pageNumber="112" refString="Leal-Zanchet, A. M. & Carbayo, F. (2001) Two new species of land planarians (Platyhelminthes, Tricladida, Terricola) of South Brazil. Journal of Zoology, 253, 433 - 446. https: // doi. org / 10.1017 / S 0952836901000401" type="journal article" year="2001">Leal-Zanchet & Carbayo 2001</bibRefCitation>
|
||
), whereas in
|
||
<taxonomicName id="4C3E71F9FFF3FFCDBFC7917DCE32FDD7" authorityName="Negrete & Brusa" authorityYear="2017" box="[750,860,584,608]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="13" pageNumber="112" phylum="Platyhelminthes" rank="species" species="negrita">
|
||
<emphasis id="B94AD668FFF3FFCDBFC7917DCE32FDD7" box="[750,860,584,608]" italics="true" pageId="13" pageNumber="112">I. negrita</emphasis>
|
||
</taxonomicName>
|
||
, they emerge from the latero-ventral face of the ovaries. In the three species, the ovovitelline ducts join dorsally, at the level of the gonopore or slightly behind it, with some intraspecific variation, as observed both in
|
||
<taxonomicName id="4C3E71F9FFF3FFCDBE3F91A5CEECFD2F" authority="Negrete & Brusa, 2017" authorityName="Negrete & Brusa" authorityYear="2017" box="[790,898,656,680]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="13" pageNumber="112" phylum="Platyhelminthes" rank="species" species="negrita" status="sp. nov.">
|
||
<emphasis id="B94AD668FFF3FFCDBE3F91A5CEECFD2F" box="[790,898,656,680]" italics="true" pageId="13" pageNumber="112">I. negrita</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A2796B13FFF3FFCDBEA491BBCE85FD20" box="[909,1003,655,680]" pageId="13" pageNumber="112" rank="species">
|
||
<emphasis id="B94AD668FFF3FFCDBEA491BBCE85FD20" bold="true" box="[909,1003,655,680]" pageId="13" pageNumber="112">sp. nov.</emphasis>
|
||
</taxonomicNameLabel>
|
||
and the Brazilian species. The same applies to the point where the female genital canal (which is extremely short in all species) opens into the female atrium. In the largest specimen of
|
||
<taxonomicName id="4C3E71F9FFF3FFCDBF3091EDCFECFD67" authority="Negrete & Brusa, 2017" authorityName="Negrete & Brusa" authorityYear="2017" box="[537,642,728,752]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="13" pageNumber="112" phylum="Platyhelminthes" rank="species" species="negrita" status="sp. nov.">
|
||
<emphasis id="B94AD668FFF3FFCDBF3091EDCFECFD67" box="[537,642,728,752]" italics="true" pageId="13" pageNumber="112">I. negrita</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A2796B13FFF3FFCDBFA391E3CF8AFD78" box="[650,740,727,752]" pageId="13" pageNumber="112" rank="species">
|
||
<emphasis id="B94AD668FFF3FFCDBFA391E3CF8AFD78" bold="true" box="[650,740,727,752]" pageId="13" pageNumber="112">sp. nov.</emphasis>
|
||
</taxonomicNameLabel>
|
||
, the female canal opens into the most distal part of the dorsal wall of the female atrium, whereas in the smaller specimens, the female canal opens into the distal third of the female atrium but not terminally. According to the schematic drawings of specimens studied by
|
||
<bibRefCitation id="EFAF778BFFF3FFCDB9C49014C8F6FCB0" author="Marcus" box="[1261,1432,799,824]" pageId="13" pageNumber="112" refString="Marcus, E. (1951) Turbellaria brasileiros (9). Boletins da Faculdade de Filosofia, Ciencias e Letras da Universidade Sao Paulo, Serie Zoologia, 16, 5 - 215. https: // doi. org / 10.11606 / issn. 2526 - 4877. bsffclzoologia. 1951.125221" type="journal article" year="1951">Marcus (1951)</bibRefCitation>
|
||
, something similar appears to occur in
|
||
<taxonomicName id="4C3E71F9FFF3FFCDBF649071CFAEFCD4" box="[589,704,837,860]" class="Turbellaria" family="Geoplanidae" genus="Imbira" higherTaxonomySource="GBIF" kingdom="Animalia" order="Tricladida" pageId="13" pageNumber="112" phylum="Platyhelminthes" rank="species" species="marcusi">
|
||
<emphasis id="B94AD668FFF3FFCDBF649071CFAEFCD4" box="[589,704,837,860]" italics="true" pageId="13" pageNumber="112">I. marcusi</emphasis>
|
||
</taxonomicName>
|
||
. However, Marcus reconstructed the copulatory apparatus of an animal after copulation, therefore mature, with the female canal opening in the middle third of the female atrium.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |