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<mods:title id="457912721997751FD7C6ADD8A7DB3F39">Molecular screening of tsetse flies and cattle reveal different Trypanosoma species including T. grayi and T. theileri in northern Cameroon</mods:title>
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<mods:namePart id="2C4B0832B06901ED2F80E1A681D0FEF7">Webel, Judcth Sophce</mods:namePart>
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<mods:namePart id="DFC56526A7E96267E9A0D3962D1036A0">Bum, Elcmabeth Ngo</mods:namePart>
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<treatment id="03DA9B06FF82B46BD584FE64A2ADFEEA" ID-DOI="http://doi.org/10.5281/zenodo.11645813" ID-Zenodo-Dep="11645813" LSID="urn:lsid:plazi:treatment:03DA9B06FF82B46BD584FE64A2ADFEEA" httpUri="http://treatment.plazi.org/id/03DA9B06FF82B46BD584FE64A2ADFEEA" lastPageNumber="6" pageId="5" pageNumber="6">
<subSubSection id="C369799BFF82B46BD584FE64A081FDAB" box="[151,485,494,516]" pageId="5" pageNumber="6" type="nomenclature">
<paragraph id="8BCC2A10FF82B46BD584FE64A081FDAB" blockId="5.[151,776,494,965]" box="[151,485,494,516]" pageId="5" pageNumber="6">
<heading id="D0849D7CFF82B46BD584FE64A081FDAB" bold="true" box="[151,485,494,516]" fontSize="9" level="2" pageId="5" pageNumber="6" reason="7">
<emphasis id="B907F602FF82B46BD584FE64A081FDAB" bold="true" box="[151,485,494,516]" pageId="5" pageNumber="6">
<taxonomicName id="4C735193FF82B46BD584FE64A040FDAB" box="[151,292,494,516]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD584FE64A040FDAB" bold="true" box="[151,292,494,516]" italics="true" pageId="5" pageNumber="6">Trypanosoma</emphasis>
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species identified
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<paragraph id="8BCC2A10FF82B46BD584FD87A08AFCEA" blockId="5.[151,776,494,965]" pageId="5" pageNumber="6">
The cdentcfccatcon of trypanosomes was performed by
<collectionCode id="ED62B2D5FF82B46BD584FDA7A1ACFDEA" box="[151,200,557,581]" pageId="5" pageNumber="6">PCR</collectionCode>
amplcfccatcon of
<collectionCode id="ED62B2D5FF82B46BD490FDA7A0D4FDEA" box="[387,432,557,581]" pageId="5" pageNumber="6">ITS</collectionCode>
1. Inctcally, trypanosome specces were prelcmcnarcly asscgned accordcng to the band scze of the product ycelded. As descrcbed cn the lcterature [
<bibRefCitation id="EFE257E1FF82B46BD7CEFDE7A39FFD2A" author="Adamm ER &amp; Malele II &amp; Mmangc AR &amp; Gcbmon WC" box="[733,763,621,645]" pageId="5" pageNumber="6" pagination="103 - 9" refId="ref12412" refString="24. Adamm ER, Malele II, Mmangc AR, Gcbmon WC. Tlspanomome cdentcfccatcon cn wcld tmetme populatconm cn Tanzanca umcng genelcc plcmelm to amplcfs the lcbomomal RNA ITS 1 legcon. Acta Tlop. 2006; 100: 103 - 9." type="journal article" year="2006">24</bibRefCitation>
], sczes cn the range between 550 and 700 bp, 400
<emphasis id="B907F602FF82B46BD7B8FD06A3DFFD0B" box="[683,699,652,676]" italics="true" pageId="5" pageNumber="6"></emphasis>
450 bp and 198
<emphasis id="B907F602FF82B46BD5E2FD26A065FD6B" box="[241,257,684,708]" italics="true" pageId="5" pageNumber="6"></emphasis>
250 bp were conscdered to come from
<taxonomicName id="4C735193FF82B46BD7ABFD27A187FD4B" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="congolense">
<emphasis id="B907F602FF82B46BD7ABFD27A187FD4B" italics="true" pageId="5" pageNumber="6">T. congolense</emphasis>
</taxonomicName>
kclcfc and
<taxonomicName id="4C735193FF82B46BD446FD47A081FD4B" box="[341,485,716,740]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="congolense">
<emphasis id="B907F602FF82B46BD446FD47A081FD4B" box="[341,485,716,740]" italics="true" pageId="5" pageNumber="6">T. congolense</emphasis>
</taxonomicName>
forest/savannah,
<taxonomicName id="4C735193FF82B46BD7B6FD47A262FD4B" box="[677,774,716,740]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="brucei">
<emphasis id="B907F602FF82B46BD7B6FD47A262FD4B" box="[677,774,716,740]" italics="true" pageId="5" pageNumber="6">T. brucei</emphasis>
</taxonomicName>
ssp., and
<taxonomicName id="4C735193FF82B46BD416FD67A006FCAB" box="[261,354,749,772]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="vivax">
<emphasis id="B907F602FF82B46BD416FD67A006FCAB" box="[261,354,749,772]" italics="true" pageId="5" pageNumber="6">T. vivax</emphasis>
</taxonomicName>
, respectcvely. Also, unexpected band sczes of about 320 and 100 bp were amplcfced (Fcg. 2b, Addctconal fcle 1:
<tableCitation id="C6F11FABFF82B46BD445FCA7A0B0FCEA" box="[342,468,813,837]" captionStart="Table 1" captionStartId="3.[151,210,1246,1267]" captionTargetPageId="3" captionText="Table 1 Plcmelm umed cn thcm mtuds to amplcfs dcffelent Trypanosoma mpeccem" httpUri="http://table.plazi.org/id/DF0C7A98FF84B46DD584FB54A21CFB5C" pageId="5" pageNumber="6" tableUuid="DF0C7A98FF84B46DD584FB54A21CFB5C">Tables S1-S</tableCitation>
<tableCitation id="C6F11FABFF82B46BD4C7FCA7A085FCEA" box="[468,481,813,837]" captionStart="Table 3" captionStartId="6.[151,210,1450,1471]" captionTargetPageId="6" captionText="Table 3 Pelcentage mequence cdentcts matlcx compalcng T. grayi and T. theileri mequencem flom cattle blood mamplem" httpUri="http://table.plazi.org/id/DF0C7A98FF81B468D584FA20A598FA10" pageId="5" pageNumber="6" tableUuid="DF0C7A98FF81B468D584FA20A598FA10">3</tableCitation>
).
</paragraph>
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To further check the cdentcfccatcon, samples were subjected to
<collectionCode id="ED62B2D5FF82B46BD43BFCE7A03DFC2A" box="[296,345,877,901]" pageId="5" pageNumber="6">PCR</collectionCode>
wcth speccfcc prcmers where avaclable. Fcnally, representatcve
<collectionCode id="ED62B2D5FF82B46BD4B2FC07A0B6FC0A" box="[417,466,909,933]" pageId="5" pageNumber="6">PCR</collectionCode>
products were subcloned and sequenced for fcnal confcrmatcon or more detacled
</paragraph>
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<emphasis id="B907F602FF82B46BD5A2FB9AA308FB88" bold="true" box="[177,620,1040,1074]" pageId="5" pageNumber="6">
a
<taxonomicName id="4C735193FF82B46BD5E1FBAAA0A6FB9D" box="[242,450,1056,1074]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="undetermined">Trypanosoma Species</taxonomicName>
<collectionCode id="ED62B2D5FF82B46BD711FB9FA340FB88" box="[514,548,1045,1063]" pageId="5" pageNumber="6">ITS</collectionCode>
1 ampl -
</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD71EFBA1A304FB92" blockId="5.[167,758,1040,1938]" box="[525,608,1067,1085]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD71EFBA1A304FB92" bold="true" box="[525,608,1067,1085]" pageId="5" pageNumber="6">icon size</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD5E1FBCFA0D8FBF8" blockId="5.[167,758,1040,1938]" box="[242,444,1093,1111]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD5E1FBCFA0D8FBF8" bold="true" box="[242,444,1093,1111]" pageId="5" pageNumber="6">
<taxonomicName id="4C735193FF82B46BD5E1FBCFA026FBF8" box="[242,322,1093,1111]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="brucei">T. brucei</taxonomicName>
rhodesiense
</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD5E1FBEBA323FBDC" blockId="5.[167,758,1040,1938]" box="[242,583,1121,1139]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD5E1FBEBA323FBDC" bold="true" box="[242,583,1121,1139]" pageId="5" pageNumber="6">
<taxonomicName id="4C735193FF82B46BD5E1FBEBA026FBDC" box="[242,322,1121,1139]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="brucei">T. brucei</taxonomicName>
gambiense 426
</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD5E1FBF7A0E7FB20" blockId="5.[167,758,1040,1938]" box="[242,387,1149,1167]" pageId="5" pageNumber="6">
<taxonomicName id="4C735193FF82B46BD5E1FBF7A0E7FB20" box="[242,387,1149,1167]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="2" phylum="Euglenozoa" rank="subSpecies" species="brucei" subSpecies="brucei">
<emphasis id="B907F602FF82B46BD5E1FBF7A0E7FB20" bold="true" box="[242,387,1149,1167]" pageId="5" pageNumber="6">T. brucei brucei</emphasis>
</taxonomicName>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD5E1FB12A323FB05" blockId="5.[167,758,1040,1938]" box="[242,583,1176,1194]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD5E1FB12A323FB05" bold="true" box="[242,583,1176,1194]" pageId="5" pageNumber="6">
<taxonomicName id="4C735193FF82B46BD5E1FB12A010FB05" box="[242,372,1176,1194]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="congolense">T. congolense</taxonomicName>
savannah 645
</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD5E1FB3EA323FB69" blockId="5.[167,758,1040,1938]" box="[242,583,1204,1222]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD5E1FB3EA323FB69" bold="true" box="[242,583,1204,1222]" pageId="5" pageNumber="6">
<taxonomicName id="4C735193FF82B46BD5E1FB3EA010FB69" box="[242,372,1204,1222]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="congolense">T. congolense</taxonomicName>
kilifi 562
</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD5E1FB47A010FB70" blockId="5.[167,758,1040,1938]" box="[242,372,1229,1247]" pageId="5" pageNumber="6">
<taxonomicName id="4C735193FF82B46BD5E1FB47A010FB70" box="[242,372,1229,1247]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="congolense">
<emphasis id="B907F602FF82B46BD5E1FB47A010FB70" bold="true" box="[242,372,1229,1247]" pageId="5" pageNumber="6">T. congolense</emphasis>
</taxonomicName>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD735FB52A323FB45" blockId="5.[167,758,1040,1938]" box="[550,583,1240,1258]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD735FB52A323FB45" bold="true" box="[550,583,1240,1258]" pageId="5" pageNumber="6">662</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD5E1FB6EA011FB59" blockId="5.[167,758,1040,1938]" box="[242,373,1252,1270]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD5E1FB6EA011FB59" bold="true" box="[242,373,1252,1270]" pageId="5" pageNumber="6">riverine/forest</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD5E1FB77A323FAA0" blockId="5.[167,758,1040,1938]" box="[242,583,1277,1295]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD5E1FB77A323FAA0" bold="true" box="[242,583,1277,1295]" pageId="5" pageNumber="6">
<taxonomicName id="4C735193FF82B46BD5E1FB77A05CFAA0" box="[242,312,1277,1295]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="vivax">T. vivax</taxonomicName>
198
</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD5E1FA93A323FA84" blockId="5.[167,758,1040,1938]" box="[242,583,1305,1323]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD5E1FA93A323FA84" bold="true" box="[242,583,1305,1323]" pageId="5" pageNumber="6">
<taxonomicName id="4C735193FF82B46BD5E1FA93A052FA84" box="[242,310,1305,1323]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="grayi">T. grayi</taxonomicName>
317
</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD5E1FABEA323FAE9" blockId="5.[167,758,1040,1938]" box="[242,583,1332,1350]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD5E1FABEA323FAE9" bold="true" box="[242,583,1332,1350]" pageId="5" pageNumber="6">
<taxonomicName id="4C735193FF82B46BD5E1FABEA023FAE9" box="[242,327,1332,1350]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="theileri">T. theileri</taxonomicName>
349
</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD5A2FAD1A1A1FAD6" blockId="5.[167,758,1040,1938]" box="[177,197,1371,1401]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD5A2FAD1A1A1FAD6" bold="true" box="[177,197,1371,1401]" pageId="5" pageNumber="6">b</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD5ACFAF4A3F9FA3F" blockId="5.[167,758,1040,1938]" box="[191,669,1406,1424]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD5ACFAF4A3F9FA3F" bold="true" box="[191,669,1406,1424]" pageId="5" pageNumber="6">M 1 2 3 4 5 6 7 8 9 10 C M</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD7AAFA37A389FA63" blockId="5.[167,758,1040,1938]" box="[697,749,1469,1484]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD7AAFA37A389FA63" bold="true" box="[697,749,1469,1484]" pageId="5" pageNumber="6">500 bp</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD7AAFA5EA389FA4C" blockId="5.[167,758,1040,1938]" box="[697,749,1492,1507]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD7AAFA5EA389FA4C" bold="true" box="[697,749,1492,1507]" pageId="5" pageNumber="6">250 bp</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD7AAF989A380F9BD" blockId="5.[167,758,1040,1938]" box="[697,740,1539,1554]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD7AAF989A380F9BD" bold="true" box="[697,740,1539,1554]" pageId="5" pageNumber="6">50 bp</emphasis>
</paragraph>
<paragraph id="8BCC2A10FF82B46BD5B4F9B5A3A8F977" blockId="5.[167,758,1040,1938]" pageId="5" pageNumber="6">
<emphasis id="B907F602FF82B46BD5B4F9B5A1B8F9FD" bold="true" box="[167,220,1599,1618]" pageId="5" pageNumber="6">Fig. 2</emphasis>
<collectionCode id="ED62B2D5FF82B46BD5F1F9B5A19BF9FD" box="[226,255,1599,1618]" pageId="5" pageNumber="6">ITS</collectionCode>
1 amplcconm of
<taxonomicName id="4C735193FF82B46BD46CF9B5A08BF9FD" box="[383,495,1599,1618]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="genus">
<emphasis id="B907F602FF82B46BD46CF9B5A08BF9FD" box="[383,495,1599,1618]" italics="true" pageId="5" pageNumber="6">Trypanosoma</emphasis>
</taxonomicName>
mpeccem.
<emphasis id="B907F602FF82B46BD72EF9B5A32CF9FD" bold="true" box="[573,584,1599,1618]" pageId="5" pageNumber="6">a</emphasis>
Amplccon mczem expected fol amplcfccatcon wcth genelcc plcmelm
<collectionCode id="ED62B2D5FF82B46BD756F9D0A306F9C2" box="[581,610,1626,1645]" pageId="5" pageNumber="6">ITS</collectionCode>
1-InF and
<collectionCode id="ED62B2D5FF82B46BD7A7F9D0A3B5F9C2" box="[692,721,1626,1645]" pageId="5" pageNumber="6">ITS</collectionCode>
1-InR wele calculated flom avaclable databame mequencem and clommchecked wcth mequencem flom mcleened mamplem.
<emphasis id="B907F602FF82B46BD4EBF905A360F90D" bold="true" box="[504,516,1679,1698]" pageId="5" pageNumber="6">b</emphasis>
<collectionCode id="ED62B2D5FF82B46BD719F905A34FF90D" box="[522,555,1679,1698]" pageId="5" pageNumber="6">PCR</collectionCode>
wam pelfolmed wcth leplementatcve mamplem contacncng DNA of the cndccated olcgcn and genelcc (
<emphasis id="B907F602FF82B46BD5E3F94FA19EF977" bold="true" box="[240,250,1733,1752]" pageId="5" pageNumber="6">a</emphasis>
) ol mpeccfcc plcmelm (
<tableCitation id="C6F11FABFF82B46BD4B5F94FA085F977" box="[422,481,1733,1752]" captionStart="Table 1" captionStartId="3.[151,210,1246,1267]" captionTargetPageId="3" captionText="Table 1 Plcmelm umed cn thcm mtuds to amplcfs dcffelent Trypanosoma mpeccem" httpUri="http://table.plazi.org/id/DF0C7A98FF84B46DD584FB54A21CFB5C" pageId="5" pageNumber="6" tableUuid="DF0C7A98FF84B46DD584FB54A21CFB5C">Table 1</tableCitation>
). Lane M: Malkel GeneRulel
</paragraph>
<paragraph id="8BCC2A10FF82B46BD5B4F955A350F83D" blockId="5.[167,758,1040,1938]" pageId="5" pageNumber="6">
50 bp Laddel (Thelmo Sccentcfcc); Lane 1:
<collectionCode id="ED62B2D5FF82B46BD4ECF955A374F95D" box="[511,528,1759,1778]" country="Japan" name="Nagao Institute" pageId="5" pageNumber="6">NI</collectionCode>
100 (genelcc plcmelm); Lane 2: Bodoncd (genelcc plcmelm); Lane 3:
<taxonomicName id="4C735193FF82B46BD4C8F970A372F8A2" box="[475,534,1786,1805]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="vivax">
<emphasis id="B907F602FF82B46BD4C8F970A372F8A2" box="[475,534,1786,1805]" italics="true" pageId="5" pageNumber="6">T. vivax</emphasis>
</taxonomicName>
(genelcc plcmelm); Lane 4:
<taxonomicName id="4C735193FF82B46BD5B4F89EA184F888" box="[167,224,1812,1831]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="grayi">
<emphasis id="B907F602FF82B46BD5B4F89EA184F888" box="[167,224,1812,1831]" italics="true" pageId="5" pageNumber="6">T. grayi</emphasis>
</taxonomicName>
(genelcc plcmelm); Lane 5:
<taxonomicName id="4C735193FF82B46BD4A8F89EA365F888" box="[443,513,1812,1831]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="theileri">
<emphasis id="B907F602FF82B46BD4A8F89EA365F888" box="[443,513,1812,1831]" italics="true" pageId="5" pageNumber="6">T. theileri</emphasis>
</taxonomicName>
(genelcc plcmelm); Lane 6:
<taxonomicName id="4C735193FF82B46BD5B4F8A5A18DF8ED" box="[167,233,1839,1858]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="brucei">
<emphasis id="B907F602FF82B46BD5B4F8A5A18DF8ED" box="[167,233,1839,1858]" italics="true" pageId="5" pageNumber="6">T. brucei</emphasis>
</taxonomicName>
mmp. (genelcc plcmelm); Lane 7:
<taxonomicName id="4C735193FF82B46BD4F4F8A5A345F8ED" box="[487,545,1839,1858]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="grayi">
<emphasis id="B907F602FF82B46BD4F4F8A5A345F8ED" box="[487,545,1839,1858]" italics="true" pageId="5" pageNumber="6">T. grayi</emphasis>
</taxonomicName>
(mpeccfcc plcmelm); Lane 8:
<taxonomicName id="4C735193FF82B46BD5B4F8C0A076F8F2" box="[167,274,1866,1885]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="congolense">
<emphasis id="B907F602FF82B46BD5B4F8C0A076F8F2" box="[167,274,1866,1885]" italics="true" pageId="5" pageNumber="6">T. congolense</emphasis>
</taxonomicName>
(mpeccfcc plcmelm); Lane 9:
<taxonomicName id="4C735193FF82B46BD4FEF8C0A33DF8F2" box="[493,601,1866,1885]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="congolense">
<emphasis id="B907F602FF82B46BD4FEF8C0A33DF8F2" box="[493,601,1866,1885]" italics="true" pageId="5" pageNumber="6">T. congolense</emphasis>
</taxonomicName>
folemt (mpeccfcc plcmelm); Lane 10:
<taxonomicName id="4C735193FF82B46BD42DF8EEA0CDF8D8" box="[318,425,1892,1911]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="congolense">
<emphasis id="B907F602FF82B46BD42DF8EEA0CDF8D8" box="[318,425,1892,1911]" italics="true" pageId="5" pageNumber="6">T. congolense</emphasis>
</taxonomicName>
folemt and
<taxonomicName id="4C735193FF82B46BD71BF8EEA317F8D8" box="[520,627,1892,1911]" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="species" species="congolense">
<emphasis id="B907F602FF82B46BD71BF8EEA317F8D8" box="[520,627,1892,1911]" italics="true" pageId="5" pageNumber="6">T. congolense</emphasis>
</taxonomicName>
kclcfc (mpeccfcc plcmelm); Lane C: contlol wcthout DNA template
</paragraph>
<paragraph id="8BCC2A10FF82B46BD63EFF67A2ADFEEA" blockId="5.[813,1436,237,325]" pageId="5" pageNumber="6">
cdentcfccatcon. The fragment sczes and respectcve
<taxonomicName id="4C735193FF82B46BD043FF67A2E5FE8B" class="Kinetoplastea" family="Trypanosomatidae" genus="Trypanosoma" kingdom="Protozoa" order="Trypanosomatida" pageId="5" pageNumber="6" phylum="Euglenozoa" rank="genus">
<emphasis id="B907F602FF82B46BD043FF67A2E5FE8B" italics="true" pageId="5" pageNumber="6">Trypanosoma</emphasis>
</taxonomicName>
specces are lcsted cn Fcg. 2 and Addctconal fcle 1:
<tableCitation id="C6F11FABFF82B46BD63EFEA7A2C8FEEA" box="[813,940,301,325]" captionStart="Table 1" captionStartId="3.[151,210,1246,1267]" captionTargetPageId="3" captionText="Table 1 Plcmelm umed cn thcm mtuds to amplcfs dcffelent Trypanosoma mpeccem" httpUri="http://table.plazi.org/id/DF0C7A98FF84B46DD584FB54A21CFB5C" pageId="5" pageNumber="6" tableUuid="DF0C7A98FF84B46DD584FB54A21CFB5C">Tables S1-S</tableCitation>
<tableCitation id="C6F11FABFF82B46BD6BFFEA7A2D8FEEA" box="[940,956,301,325]" captionStart="Table 4" captionStartId="7.[151,210,237,258]" captionTargetPageId="7" captionText="Table 4 Dcmtlcbutcon of the Trypanosoma mpeccem cn Glossina mpeccem cn the gut. Total numbel of Trypanosoma mpeccem detected" httpUri="http://table.plazi.org/id/DF0C7A98FF80B469D584FF67A400FEAD" pageId="5" pageNumber="6" tableUuid="DF0C7A98FF80B469D584FF67A400FEAD">4</tableCitation>
).
</paragraph>
</subSubSection>
</treatment>
</document>