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<document ID-CLB-Dataset="21804" ID-DOI="http://dx.doi.org/10.3897/mycokeys.67.52055" ID-GBIF-Dataset="ad20a203-0984-4ab6-bdb8-71b078dba7af" ID-PMC="PMC7270078" ID-Pensoft-Pub="1314-4049-67-55" ID-Pensoft-UUID="CE9642BF68865BC2B7929C4F7076DCAE" ID-PubMed="32547306" ModsDocID="1314-4049-67-55" checkinTime="1590646543563" checkinUser="pensoft" docAuthor="Valle, Laia Guardia &amp; Stoianova, Desislava" docDate="2020" docId="78D22DC9A2A85535A18740E3A3D5CD0E" docLanguage="en" docName="MycoKeys 67: 55-80" docOrigin="MycoKeys 67" docSource="http://dx.doi.org/10.3897/mycokeys.67.52055" docTitle="Graminella bulbosa Leger &amp; Gauthier, 1937 ex Manier 1962" docType="treatment" docVersion="6" id="CE9642BF68865BC2B7929C4F7076DCAE" lastPageNumber="55" masterDocId="CE9642BF68865BC2B7929C4F7076DCAE" masterDocTitle="First record of Harpellales, Orphellales (Kickxellomycotina) and Amoebidiales (Mesomycetozoea) from Bulgaria, including a new species of Glotzia" masterLastPageNumber="80" masterPageNumber="55" pageNumber="55" updateTime="1732687699600" updateUser="ExternalLinkService">
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<mods:title id="EF221B48C8E375BE24312BB4D2A1C243">First record of Harpellales, Orphellales (Kickxellomycotina) and Amoebidiales (Mesomycetozoea) from Bulgaria, including a new species of Glotzia</mods:title>
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<mods:namePart id="97D784F4FC3379ECEE0C4032F6C93364">Valle, Laia Guardia</mods:namePart>
<mods:affiliation id="45D7FCA35E3F37143C88FA39C1D06B2F">Unitat de Botanica, Dept. Biologia Animal, Biologia Vegetal i d'Ecologia. Fac. Biociences. Universitat Autonoma de Barcelona. 08193 - Bellaterra (Barcelona), Spain</mods:affiliation>
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<mods:nameIdentifier id="BEC9A28322D3337CDF3626615C014FDE" type="email">laia.guardia@uab.cat</mods:nameIdentifier>
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<mods:namePart id="11ADC192C76EE75E5BFDC2C828B45339">Stoianova, Desislava</mods:namePart>
<mods:affiliation id="D6CD1CC1582AFE6380F34EEFE91A023D">Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences. Sofia, Bulgaria</mods:affiliation>
<mods:nameIdentifier id="BA4089C411D2AE325D3AC8F90C7AA676" type="orcid">https://orcid.org/0000-0001-8040-7158</mods:nameIdentifier>
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<mods:date id="A188C9EF180B8F43AA5CEBD5E4CFEEBD">2020</mods:date>
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<mods:identifier id="C5A2272ACFB166FDB8CA247294C26B71" type="DOI">http://dx.doi.org/10.3897/mycokeys.67.52055</mods:identifier>
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<treatment id="78D22DC9A2A85535A18740E3A3D5CD0E" ID-GBIF-Taxon="164363115" LSID="urn:lsid:plazi:treatment:78D22DC9A2A85535A18740E3A3D5CD0E" httpUri="http://treatment.plazi.org/id/78D22DC9A2A85535A18740E3A3D5CD0E" lastPageNumber="55" pageId="0" pageNumber="55" scope_order="Harpellales">
<subSubSection id="F82C30F90A7162DCAF725EF61732EFD8" pageId="0" pageNumber="55" type="nomenclature">
<paragraph id="C689437B39CDA65F3DC9E90A55EB099E" pageId="0" pageNumber="55">
<taxonomicName id="FB5931F62BBA50F6C387A6E9E028A3A0" ID-CoL="3H5TJ" LSID="78D22DC9-A2A8-5535-A187-40E3A3D5CD0E" authority="Leger &amp; Gauthier, 1937 ex Manier, 1962." authorityName="Leger &amp; Gauthier, 1937 ex Manier" authorityYear="1962" class="Kickxellomycetes" family="Legeriomycetaceae" genus="Graminella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Graminella bulbosa" order="Harpellales" pageId="0" pageNumber="55" phylum="Zygomycota" rank="species" species="bulbosa">
Graminella bulbosa
<normalizedToken id="67A2DAC97B95D5BFEDCE95922CE88052" originalValue="Léger">Leger</normalizedToken>
&amp; Gauthier, 1937 ex Manier, 1962.
</taxonomicName>
<figureCitation id="09CCF6B9844DC7334BA2AC094FFD86F8" captionStart="Figures 1116" captionStartId="F3" captionText="Figures 11 - 16. Various species of Harpellales. 11 - 13 Graminella microspora from Baetidae nymphs: 11 thallus overview, with inflated basal cell structures from which propagules arise or are extruded (note: one empty basal cell which has extruded its cellular content, arrow); young zygospores can also be observed (arrowhead) 12 a cluster of basal cells 13 fertile branch with a long series of minute trichospores; 14, 15 Harpella melusinae from Simuliidae larvae: 14 generative cells and allantoids or curved trichospores 15 basal cell and small conic holdfast 16 Legeriomyces ramosus from Baetidae nymphs, zygospores and zygosporophores. Scale bars: 25 μm in all figures." figureDoi="10.3897/mycokeys.67.52055.figures11-16" httpUri="https://binary.pensoft.net/fig/414541" pageId="0" pageNumber="55">Figs 11-13</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="3B2DF5D40D4C2C7E98315E11E4E52C19" pageId="0" pageNumber="55" type="materials_examined">
<paragraph id="C152BE5112131A52515A35DEA8303770" pageId="0" pageNumber="55">Specimens examined.</paragraph>
<paragraph id="662C291C960C3C5AF0021449D5A41210" pageId="0" pageNumber="55">Site 2: slide BUL-2-5; Site 8: slides BUL-8-1, BUL-8-2.</paragraph>
</subSubSection>
<subSubSection id="A17F8121ACEAE2FC873AB1BC5821922D" pageId="0" pageNumber="55" type="notes">
<paragraph id="ABAC3B2A1E23317CBB33B24E84D3F9D0" pageId="0" pageNumber="55">Notes.</paragraph>
<paragraph id="5F7C05365E4D91920A3F257F9842E9FE" pageId="0" pageNumber="55">
This species is characterised by the unusual formation of vegetative propagules from the bulbous basal cells (Fig.
<figureCitation id="71053C80DCF095BBDF22EB9819EA6F3A" captionStart="Figures 1116" captionStartId="F3" captionText="Figures 11 - 16. Various species of Harpellales. 11 - 13 Graminella microspora from Baetidae nymphs: 11 thallus overview, with inflated basal cell structures from which propagules arise or are extruded (note: one empty basal cell which has extruded its cellular content, arrow); young zygospores can also be observed (arrowhead) 12 a cluster of basal cells 13 fertile branch with a long series of minute trichospores; 14, 15 Harpella melusinae from Simuliidae larvae: 14 generative cells and allantoids or curved trichospores 15 basal cell and small conic holdfast 16 Legeriomyces ramosus from Baetidae nymphs, zygospores and zygosporophores. Scale bars: 25 μm in all figures." figureDoi="10.3897/mycokeys.67.52055.figures11-16" httpUri="https://binary.pensoft.net/fig/414541" pageId="0" pageNumber="55">12</figureCitation>
), a feature only shared with the related genus
<taxonomicName id="496662FAD86EC3336FC2D16191082050" authorityName="R.W.Lichtwardt" authorityYear="1983" class="Kickxellomycetes" family="Legeriomycetaceae" genus="Gauthieromyces" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Gauthieromyces" order="Harpellales" pageId="0" pageNumber="55" phylum="Zygomycota" rank="genus">
<emphasis id="542BB8D6337B84E0BC4B0DC77D3731A9" italics="true" pageId="0" pageNumber="55">Gauthieromyces</emphasis>
</taxonomicName>
(
<bibRefCitation id="47534BAF39554443715A798BF70184D7" author="Lichtwardt, RW" journalOrPublisher="Mycotaxon" pageId="0" pageNumber="55" pagination="213 - 215" refId="B21" refString="Lichtwardt, RW, 1983. Gauthieromyces, a new genus of Harpellales based on Genistella microspora. Mycotaxon 17: 213 - 215" title="Gauthieromyces, a new genus of Harpellales based on Genistella microspora." volume="17" year="1983">Lichtwardt 1983</bibRefCitation>
).
<taxonomicName id="E8A79EF5275C82ECD91277030BDAF691" authorityName="Leger &amp; Gauthier, 1937 ex Manier" authorityYear="1962" class="Kickxellomycetes" family="Legeriomycetaceae" genus="Graminella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Graminella bulbosa" order="Harpellales" pageId="0" pageNumber="55" phylum="Zygomycota" rank="species" species="bulbosa">
<emphasis id="73728443676AB9301E60DF9D7A942ED7" italics="true" pageId="0" pageNumber="55">Graminella bulbosa</emphasis>
</taxonomicName>
was described from France (
<bibRefCitation id="D86A41C921DCD66F265E440D8C0B338D" author="Leger, L" journalOrPublisher="Comptes Rendus de l'Academie des Sciences, Paris" pageId="0" pageNumber="55" pagination="27 - 29" refId="B19" refString="Leger, L, Gauthier, M, 1937. Graminella bulbosa nouveau genre d'Entophyte parasite des larves d'ephemerides du genre Baetis. Comptes Rendus de l'Academie des Sciences, Paris 202: 27 - 29" title="Graminella bulbosa nouveau genre d'Entophyte parasite des larves d'ephemerides du genre Baetis." volume="202" year="1937">
<normalizedToken id="0934FCF1887A05071F14F00DC0CC3377" originalValue="Léger">Leger</normalizedToken>
and Gauthier 1937
</bibRefCitation>
; Manier 1962). The species is also known from Spain (
<bibRefCitation id="6F43CE80EF40AE9F268F2325A58F1FDB" DOI="https://doi.org/10.1080/15572536.2007.11832569" author="Valle, LG" journalOrPublisher="Mycologia" pageId="0" pageNumber="55" pagination="442 - 455" refId="B58" refString="Valle, LG, 2007. New species and summary of Iberian Harpellales. Mycologia 99: 442 - 455, DOI: https://doi.org/10.1080/15572536.2007.11832569" title="New species and summary of Iberian Harpellales." url="https://doi.org/10.1080/15572536.2007.11832569" volume="99" year="2007">Valle 2007</bibRefCitation>
), Portugal (
<bibRefCitation id="87ABEF0A37630503ADF18B610701A2B1" DOI="https://doi.org/10.3852/12-211" author="Valle, LG" journalOrPublisher="Mycologia" pageId="0" pageNumber="55" pagination="748 - 759" refId="B59" refString="Valle, LG, 2013a. New and rare Harpellales from Portugal and northwestern Iberian Peninsula: discovering the hidden mycobiota of Galicia-Tras-os-Montes region. Mycologia 105: 748 - 759, DOI: https://doi.org/10.3852/12-211" title="New and rare Harpellales from Portugal and northwestern Iberian Peninsula: discovering the hidden mycobiota of Galicia-Tras-os-Montes region." url="https://doi.org/10.3852/12-211" volume="105" year="2013 a">Valle 2013a</bibRefCitation>
) and Italy (
<bibRefCitation id="1C373AEC57E9D29A52A53C8BCDE7BAE1" DOI="https://doi.org/10.3852/12-184" author="Valle, LG" journalOrPublisher="Mycologia" pageId="0" pageNumber="55" pagination="712 - 727" refId="B67" refString="Valle, LG, Rossi, W, Santamaria, S, 2013. New species and new records of trichomycetes from Italy. Mycologia 105: 712 - 727, DOI: https://doi.org/10.3852/12-184" title="New species and new records of trichomycetes from Italy." url="https://doi.org/10.3852/12-184" volume="105" year="2013">Valle et al. 2013</bibRefCitation>
).
<taxonomicName id="1D88F9B86CD0FC1239EEFB9F8DBCBE81" authorityName="Leger &amp; Gauthier, 1937 ex Manier" authorityYear="1962" class="Kickxellomycetes" family="Legeriomycetaceae" genus="Graminella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Graminella bulbosa" order="Harpellales" pageId="0" pageNumber="55" phylum="Zygomycota" rank="species" species="bulbosa">
<emphasis id="084D5387C6B3B731105A93A83D0C8783" italics="true" pageId="0" pageNumber="55">Graminella bulbosa</emphasis>
</taxonomicName>
has been reported associated with various species of
<taxonomicName id="382C197D5D6B08F3BE3ECAD99DA48B9A" authorityName="Leach" authorityYear="1815" class="Insecta" family="Baetidae" genus="Baetis" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Baetis" order="Ephemeroptera" pageId="0" pageNumber="55" phylum="Arthropoda" rank="genus">
<emphasis id="9A2B5826EE8FE8EFAA641E2A70C912CF" italics="true" pageId="0" pageNumber="55">Baetis</emphasis>
</taxonomicName>
and related genera, very frequently within the hindgut of
<taxonomicName id="5D9E90A239D14FEAF45FE2E823582560" lsidName="B. rhodani" pageId="0" pageNumber="55" rank="species" species="rhodani">
<emphasis id="BD1687D9E8479DE03EC000046E1D45F1" italics="true" pageId="0" pageNumber="55">B. rhodani</emphasis>
</taxonomicName>
(Pictet). This species of mayfly is common and widespread in Europe and it also hosted Bulgarian specimens of
<taxonomicName id="C27BF317CC9CBFFA10D2C63763D18376" lsidName="G. bulbosa" pageId="0" pageNumber="55" rank="species" species="bulbosa">
<emphasis id="D8FF715F2A7FBCEEC2BE28EE8ED0B4BC" italics="true" pageId="0" pageNumber="55">G. bulbosa</emphasis>
</taxonomicName>
in the surveyed rivers, together with
<taxonomicName id="01826A85F3D60A7106B8C3D50773371C" lsidName="B. alpinus" pageId="0" pageNumber="55" rank="species" species="alpinus">
<emphasis id="6674C60DAB6D01D8DCD3BAF5378DE7E0" italics="true" pageId="0" pageNumber="55">B. alpinus</emphasis>
</taxonomicName>
(Pictet). In fact, the genus
<taxonomicName id="8613C39F732F00F0D4529E5695C8A6A6" authorityName="Leach" authorityYear="1815" class="Insecta" family="Baetidae" genus="Baetis" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Baetis" order="Ephemeroptera" pageId="0" pageNumber="55" phylum="Arthropoda" rank="genus">
<emphasis id="EF66BF7208B18EF0F0EF9FA00139DC2D" italics="true" pageId="0" pageNumber="55">Baetis</emphasis>
</taxonomicName>
bears different Harpellid species, including the more common
<taxonomicName id="3755CCEC751742C6F28BCDE26283C9DB" authorityName="Pouzar" authorityYear="1972" class="Kickxellomycetes" family="Legeriomycetaceae" genus="Legeriomyces" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Legeriomyces ramosus" order="Harpellales" pageId="0" pageNumber="55" phylum="Zygomycota" rank="species" species="ramosus">
<emphasis id="345149170429F714000CFBC739D85B3C" italics="true" pageId="0" pageNumber="55">Legeriomyces ramosus</emphasis>
</taxonomicName>
, occasionally sharing the same gut lumen with
<taxonomicName id="EABCB33622600BBE59429844B3C6D7C5" authorityName="Leger &amp; Gauthier, 1937 ex Manier" authorityYear="1962" class="Kickxellomycetes" family="Legeriomycetaceae" genus="Graminella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Graminella bulbosa" order="Harpellales" pageId="0" pageNumber="55" phylum="Zygomycota" rank="species" species="bulbosa">
<emphasis id="07B26921F13C047DF3D13755E320FC45" italics="true" pageId="0" pageNumber="55">Graminella bulbosa</emphasis>
</taxonomicName>
. Bulgarian specimens of
<taxonomicName id="EF8638ECB3FF10AFC65E5811CBECF3A5" lsidName="G. bulbosa" pageId="0" pageNumber="55" rank="species" species="bulbosa">
<emphasis id="9C290A7B61A5165726AE28B17F614E4C" italics="true" pageId="0" pageNumber="55">G. bulbosa</emphasis>
</taxonomicName>
show the typical small and numerous trichospores (Fig.
<figureCitation id="AC0C812B520A0604D103736ADB9F0EC9" captionStart="Figures 1116" captionStartId="F3" captionText="Figures 11 - 16. Various species of Harpellales. 11 - 13 Graminella microspora from Baetidae nymphs: 11 thallus overview, with inflated basal cell structures from which propagules arise or are extruded (note: one empty basal cell which has extruded its cellular content, arrow); young zygospores can also be observed (arrowhead) 12 a cluster of basal cells 13 fertile branch with a long series of minute trichospores; 14, 15 Harpella melusinae from Simuliidae larvae: 14 generative cells and allantoids or curved trichospores 15 basal cell and small conic holdfast 16 Legeriomyces ramosus from Baetidae nymphs, zygospores and zygosporophores. Scale bars: 25 μm in all figures." figureDoi="10.3897/mycokeys.67.52055.figures11-16" httpUri="https://binary.pensoft.net/fig/414541" pageId="0" pageNumber="55">13</figureCitation>
), measuring 8 -11
<normalizedToken id="27114457635E8D42374A23CE6754E995" originalValue="×">x</normalizedToken>
2
<normalizedToken id="03D214DAFA03B42D83D95F06D3ADC2AF" originalValue="µm">µm</normalizedToken>
in our collections. These measurements are midway between
<taxonomicName id="11E0622EECDB0297D5916F184C18E643" lsidName="G. bulbosa" pageId="0" pageNumber="55" rank="species" species="bulbosa">
<emphasis id="D683E01D3ADD2E77DB6BF57BB64E4F4B" italics="true" pageId="0" pageNumber="55">G. bulbosa</emphasis>
</taxonomicName>
and
<taxonomicName id="0D23E2A05BA2065A91AFACC96020B8EB" lsidName="G. microsporus" pageId="0" pageNumber="55" rank="species" species="microsporus">
<emphasis id="EDD52CCF3A1C4A9AC1CACCCA019902D7" italics="true" pageId="0" pageNumber="55">G. microsporus</emphasis>
</taxonomicName>
(see discussion for further information). Unfortunately, only immature zygospores were observed (Fig.
<figureCitation id="7466F88B34D8FADAE343708527AC53F8" captionStart="Figures 1116" captionStartId="F3" captionText="Figures 11 - 16. Various species of Harpellales. 11 - 13 Graminella microspora from Baetidae nymphs: 11 thallus overview, with inflated basal cell structures from which propagules arise or are extruded (note: one empty basal cell which has extruded its cellular content, arrow); young zygospores can also be observed (arrowhead) 12 a cluster of basal cells 13 fertile branch with a long series of minute trichospores; 14, 15 Harpella melusinae from Simuliidae larvae: 14 generative cells and allantoids or curved trichospores 15 basal cell and small conic holdfast 16 Legeriomyces ramosus from Baetidae nymphs, zygospores and zygosporophores. Scale bars: 25 μm in all figures." figureDoi="10.3897/mycokeys.67.52055.figures11-16" httpUri="https://binary.pensoft.net/fig/414541" pageId="0" pageNumber="55">11</figureCitation>
arrowhead).
</paragraph>
</subSubSection>
</treatment>
</document>