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<mods:title id="5596843A5CB967EDDE485046C7D30844">A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae</mods:title>
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DESCRIPTION OF
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<emphasis id="B954EA8EFFA52159DD02FB0AFBAA66B9" bold="true" italics="true" pageId="40" pageNumber="2822">LIGILACTOBACILLUS ARAFFINOSUS</emphasis>
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<emphasis id="B954EA8EFFA52159DA0BFB7AFBF96763" box="[828,1110,1260,1284]" italics="true" pageId="40" pageNumber="2822">Ligilactobacillus araffinosus</emphasis>
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(a.raf.fi.nosus. Gr. pref.
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not; N.L. masc. adj.
<emphasis id="B954EA8EFFA52159DA9BFA9DFBBE6744" box="[940,1041,1291,1315]" italics="true" pageId="40" pageNumber="2822">raffinosus</emphasis>
of raffinose; N.L. masc. adj.
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<emphasis id="B954EA8EFFA52159DC75FA9DFA1B6744" box="[1346,1460,1291,1315]" italics="true" pageId="40" pageNumber="2822">araffinosus</emphasis>
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, not fermenting raffinose).
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Basonym:
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<emphasis id="B954EA8EFFA52159DA9AFACFFB3D6717" box="[941,1170,1369,1393]" italics="true" pageId="40" pageNumber="2822">Lactobacillus aviarius</emphasis>
subsp.
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Fujisawa
<emphasis id="B954EA8EFFA52159DA0BFAEFFCC867F7" box="[828,871,1400,1424]" italics="true" pageId="40" pageNumber="2822">et al</emphasis>
. 1985
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, 223
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; Fujisawa
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. 1986, (Effective publication: Fujisawa
<emphasis id="B954EA8EFFA52159DAA9FA0EFC6567C8" box="[926,970,1431,1455]" italics="true" pageId="40" pageNumber="2822">et al</emphasis>
., 1984, 419).
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Placement of
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<emphasis id="B954EA8EFFA52159DAFCFA50FBF767B9" box="[971,1112,1478,1502]" italics="true" pageId="40" pageNumber="2822">L. araffinosus</emphasis>
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in a separate species is supported by ANI values and digital DNADNA hybridization [
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]. The availability of the genome sequences of the
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strains of both subspecies revealed that they are only distantly related with an ANI value of 88.98%, substantially below the 9596 % threshold recognised for the species level. Furthermore, their core nucleotide identity (CNI) [
<bibRefCitation id="EFB14B6DFFA52159DDECF914FB5664FD" author="Wittouck S &amp; Wuyts S &amp; Meehan CJ &amp; van Noort V &amp; Lebeer S." box="[1243,1273,1666,1690]" pageId="40" pageNumber="2822" refId="ref60115" refString="10. Wittouck S, Wuyts S, Meehan CJ, van Noort V, Lebeer S. A genome-based species taxonomy of the Lactobacillus Genus Complex. mSystems 2019; 4: e 00264 - 19." type="journal volume" year="2019">10</bibRefCitation>
] is 91.1, which is below the 94 % threshold at which two strains are allocated to the same species. Finally, their
<emphasis id="B954EA8EFFA52159DDA0F957FB4A64BF" box="[1175,1253,1728,1752]" italics="true" pageId="40" pageNumber="2822">in silico</emphasis>
DDH value is 37.80, which additionally supports that these two subspecies should be recognised as different species [
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].
</paragraph>
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Cells are Gram- positive, non-motile, non-spore-forming rods with rounded ends, usually occurring singly or in short chains.
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<emphasis id="B954EA8EFFA52159DABDF8FAFBBB65E3" box="[906,1044,1900,1924]" italics="true" pageId="40" pageNumber="2822">L. araffinosus</emphasis>
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strains ferment trehalose and cellobiose, they do not produce acid from galactose, lactose, melibiose and raffinose [
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]. The genome size of the
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strain is 1.48 Mbp. The mol% G+C content of DNA is 38.1.
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The
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strain is
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=
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16S rRNA gene accession number:
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.
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