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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.33.23670" ID-GBIF-Dataset="42cbc858-8729-4618-9467-590ea7ca068e" ID-PMC="PMC6283267" ID-Pensoft-Pub="1314-4049-33-25" ID-PubMed="30532625" ModsDocAuthor="" ModsDocDate="2018" ModsDocID="1314-4049-33-25" ModsDocOrigin="MycoKeys 33" ModsDocTitle="Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand" checkinTime="1522305991188" checkinUser="pensoft" docAuthor="Tibpromma, Saowaluck, Hyde, Kevin D., Bhat, Jayarama D., Mortimer, Peter E., Xu, Jianchu, Promputtha, Itthayakorn, Doilom, Mingkwan, Yang, Jun-Bo, Tang, Alvin M. C. &amp; Karunarathna, Samantha C." docDate="2018" docId="F63FD24A3884F54BDFE600F0A4F6A5B8" docLanguage="en" docName="MycoKeys 33: 25-67" docOrigin="MycoKeys 33" docSource="http://dx.doi.org/10.3897/mycokeys.33.23670" docTitle="Massarina pandanicola Tibpromma &amp; K. D. Hyde, sp. nov." docType="treatment" docVersion="6" lastPageNumber="39" masterDocId="B85B6F41CC50A742FFD77F5B3B598C43" masterDocTitle="Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand" masterLastPageNumber="67" masterPageNumber="25" pageNumber="36" updateTime="1668135980046" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Tibpromma, Saowaluck</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Hyde, Kevin D.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Bhat, Jayarama D.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Mortimer, Peter E.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Xu, Jianchu</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Promputtha, Itthayakorn</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Doilom, Mingkwan</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Yang, Jun-Bo</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Tang, Alvin M. C.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Karunarathna, Samantha C.</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2018</mods:date>
<mods:detail type="volume">
<mods:number>33</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>25</mods:start>
<mods:end>67</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.33.23670</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.33.23670</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-33-25</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="142664211" LSID="urn:lsid:plazi:treatment:F63FD24A3884F54BDFE600F0A4F6A5B8" httpUri="http://treatment.plazi.org/id/F63FD24A3884F54BDFE600F0A4F6A5B8" lastPageId="14" lastPageNumber="39" pageId="11" pageNumber="36">
<subSubSection pageId="11" pageNumber="36" type="nomenclature">
<paragraph pageId="11" pageNumber="36">
<taxonomicName LSID=" MB823839" authority="Tibpromma &amp; K. D. Hyde" class="Dothideomycetes" family="Massarinaceae" genus="Massarina" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Massarina pandanicola" order="Pleosporales" pageId="11" pageNumber="36" phylum="Ascomycota" rank="species" species="pandanicola">Massarina pandanicola Tibpromma &amp; K.D. Hyde</taxonomicName>
<taxonomicNameLabel pageId="11" pageNumber="36">sp. nov.</taxonomicNameLabel>
Figure 10
</paragraph>
</subSubSection>
<subSubSection pageId="12" pageNumber="37" type="etymology">
<paragraph pageId="12" pageNumber="37">
<pageBreakToken pageId="12" pageNumber="37" start="start">Etymology</pageBreakToken>
.
</paragraph>
<paragraph pageId="12" pageNumber="37">
name referring to the host genus of the plant on which the fungus was first discovered (
<taxonomicName class="Liliopsida" family="Pandanaceae" genus="Pandanus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Pandanus" order="Pandanales" pageId="12" pageNumber="37" phylum="Tracheophyta" rank="genus">Pandanus</taxonomicName>
).
</paragraph>
</subSubSection>
<subSubSection pageId="13" pageNumber="38" type="holotype">
<paragraph pageId="13" pageNumber="38">
<pageBreakToken pageId="13" pageNumber="38" start="start">Holotype</pageBreakToken>
.
</paragraph>
<paragraph pageId="13" pageNumber="38">MFLU 18-0004</paragraph>
</subSubSection>
<subSubSection pageId="13" pageNumber="38" type="description">
<paragraph pageId="13" pageNumber="38">Description.</paragraph>
<paragraph pageId="13" pageNumber="38">
<taxonomicName genus="Colonies" lsidName="Colonies" pageId="13" pageNumber="38" rank="genus">Colonies</taxonomicName>
on PDA attaining 9 cm diam. in 4 weeks at room temperature, slow growing, white to yellow-white.
<taxonomicName genus="Mycelium" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Mycelium" pageId="13" pageNumber="38" phylum="Basidiomycota" rank="genus">Mycelium</taxonomicName>
superficial and immersed composed of septate, branched, 2.5-7
<normalizedToken originalValue="µm">µm</normalizedToken>
wide, sub-hyaline, with smooth and thick-walled hyphae. Sexual morph Undetermined. Asexual morph
<taxonomicName genus="Conidiophores" lsidName="Conidiophores" pageId="13" pageNumber="38" rank="genus">Conidiophores</taxonomicName>
12-25
<normalizedToken originalValue="µm">µm</normalizedToken>
high, 8-14
<normalizedToken originalValue="µm">µm</normalizedToken>
diam. (x̄ = 15.12
<normalizedToken originalValue="×">x</normalizedToken>
10.45
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 10), enteroblastic, phialidic, cylindrical or sub-cylindrical, sub-hyaline.
<taxonomicName class="Arthoniomycetes" family="Arthoniaceae" genus="Conidia" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Conidia" order="Arthoniales" pageId="13" pageNumber="38" phylum="Ascomycota" rank="genus">Conidia</taxonomicName>
3-5
<normalizedToken originalValue="×">x</normalizedToken>
1-3
<normalizedToken originalValue="µm">µm</normalizedToken>
(x̄ = 4.34
<normalizedToken originalValue="×">x</normalizedToken>
1.75
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 30), cylindrical, hyaline, smooth and thin-walled, aseptate, rounded ends, guttulate, without sheet or appendages.
</paragraph>
</subSubSection>
<subSubSection pageId="14" pageNumber="39" type="description">
<paragraph pageId="14" pageNumber="39">
<pageBreakToken pageId="14" pageNumber="39" start="start">Culture</pageBreakToken>
characteristics.
</paragraph>
<paragraph pageId="14" pageNumber="39">
<taxonomicName genus="Colonies" lsidName="Colonies" pageId="14" pageNumber="39" rank="genus">Colonies</taxonomicName>
on PDA (Figure 2, PE52), superficial, white to yellow-white, irregular, undulate with smooth and raised on surface media; reverse yellow-white. Generative hyphae simple-septate, branched, sub-hyaline, with guttulate cells, thin-walled. Sporulating in culture within 3 months (Figure 10).
</paragraph>
</subSubSection>
<subSubSection pageId="14" pageNumber="39" type="materials_examined">
<paragraph pageId="14" pageNumber="39">Material examined.</paragraph>
<paragraph pageId="14" pageNumber="39">
THAILAND, Chumphon, Pathio District, on healthy leaves of
<taxonomicName class="Liliopsida" family="Pandanaceae" genus="Pandanus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Pandanus" order="Pandanales" pageId="14" pageNumber="39" phylum="Tracheophyta" rank="genus">Pandanus</taxonomicName>
sp. (
<taxonomicName family="Pandanaceae" lsidName="Mycelium" pageId="14" pageNumber="39" rank="family">Pandanaceae</taxonomicName>
), 1 December 2016, S. Tibpromma PE52 (MFLU 18-0004, holotype); HKAS100854, paratype, ex-type living cultures, MFLUCC 17-0596 = KUMCC 17-0293.
</paragraph>
</subSubSection>
<subSubSection pageId="14" pageNumber="39" type="genbank numbers">
<paragraph pageId="14" pageNumber="39">Genbank numbers.</paragraph>
<paragraph pageId="14" pageNumber="39">ITS=MG646958, LSU=MG646947, SSU=MG646979, TEF1=MG646986.</paragraph>
</subSubSection>
<subSubSection pageId="14" pageNumber="39" type="notes">
<paragraph pageId="14" pageNumber="39">Notes.</paragraph>
<paragraph pageId="14" pageNumber="39">
The genus
<taxonomicName class="Dothideomycetes" family="Massarinaceae" genus="Massarina" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Massarina" order="Pleosporales" pageId="14" pageNumber="39" phylum="Ascomycota" rank="genus">Massarina</taxonomicName>
has been known as a phylogenetically diverse group in the order
<taxonomicName genus="Pleosporales" lsidName="Pleosporales" pageId="14" pageNumber="39" rank="genus">Pleosporales</taxonomicName>
based on molecular data (
<bibRefCitation author="Liew, ECY" journalOrPublisher="Mycologia" pageId="31" pageNumber="56" pagination="803 - 813" title="An evaluation of the monophyly of Massarina based on ribosomal DNA sequences." url="https://doi.org/10.1080/15572536.2003.11833174" volume="94" year="2002">Liew et al. 2002</bibRefCitation>
) and most members in
<taxonomicName class="Dothideomycetes" family="Massarinaceae" genus="Massarina" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Massarina" order="Pleosporales" pageId="14" pageNumber="39" phylum="Ascomycota" rank="genus">Massarina</taxonomicName>
except for the type species (
<taxonomicName lsidName="M. eburnea" pageId="14" pageNumber="39" rank="species" species="eburnea">M. eburnea</taxonomicName>
) are morphologically variable. The taxon,
<taxonomicName class="Dothideomycetes" family="Massarinaceae" genus="Massarina" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Massarina pandanicola" order="Pleosporales" pageId="14" pageNumber="39" phylum="Ascomycota" rank="species" species="pandanicola">Massarina pandanicola</taxonomicName>
collected from
<taxonomicName class="Liliopsida" family="Pandanaceae" genus="Pandanus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Pandanus" order="Pandanales" pageId="14" pageNumber="39" phylum="Tracheophyta" rank="genus">Pandanus</taxonomicName>
sp. in Thailand is introduced here as a new species with both morphology and phylogeny support. The morphology of the taxon showed similar conidia with
<taxonomicName class="Dothideomycetes" family="Massarinaceae" genus="Massarina" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Massarina eburnean" order="Pleosporales" pageId="14" pageNumber="39" phylum="Ascomycota" rank="species" species="eburnean">Massarina eburnean</taxonomicName>
(
<bibRefCitation author="Tanaka, K" journalOrPublisher="Studies in mycology" pageId="35" pageNumber="60" pagination="75 - 136" title="Revision of the Massarineae (Pleosporales, Dothideomycetes)." url="http://dx.doi.org/10.1016/j.simyco.2015.10.002" volume="82" year="2015">Tanaka et al. 2015</bibRefCitation>
), but based on phylogenetic analysis of combined ITS, LSU, SSU and TEF1 gene sequence data, the new taxon
<taxonomicName lsidName="M. pandanicola" pageId="14" pageNumber="39" rank="species" species="pandanicola">M. pandanicola</taxonomicName>
is well-separated from other species in
<taxonomicName class="Dothideomycetes" family="Massarinaceae" genus="Massarina" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Massarina" order="Pleosporales" pageId="14" pageNumber="39" phylum="Ascomycota" rank="genus">Massarina</taxonomicName>
(Figure 9) with high bootstrap support (79 % in ML). This is the first record of
<taxonomicName class="Dothideomycetes" family="Massarinaceae" genus="Massarina" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Massarina" order="Pleosporales" pageId="14" pageNumber="39" phylum="Ascomycota" rank="genus">Massarina</taxonomicName>
from
<taxonomicName class="Liliopsida" family="Pandanaceae" genus="Pandanus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Pandanus" order="Pandanales" pageId="14" pageNumber="39" phylum="Tracheophyta" rank="genus">Pandanus</taxonomicName>
sp.
</paragraph>
<caption pageId="14" pageNumber="39">
<paragraph pageId="14" pageNumber="39">
Figure 9. Phylogram generated from maximum likelihood analysis based on ITS, TEF1, SSU, LSU and RPB2 sequenced data. Maximum likelihood bootstrap values are given above/below the nodes. The newly generated sequences in red bold. The tree is rooted with
<taxonomicName class="Dothideomycetes" family="Pleosporaceae" genus="Alternaria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Alternaria alternata" order="Pleosporales" pageId="14" pageNumber="39" phylum="Ascomycota" rank="species" species="alternata">Alternaria alternata</taxonomicName>
and
<taxonomicName class="Dothideomycetes" family="Pleosporaceae" genus="Pleospora" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Pleospora herbarum" order="Pleosporales" pageId="14" pageNumber="39" phylum="Ascomycota" rank="species" species="herbarum">Pleospora herbarum</taxonomicName>
.
</paragraph>
</caption>
<caption pageId="14" pageNumber="39">
<paragraph pageId="14" pageNumber="39">
Figure 10.
<taxonomicName class="Dothideomycetes" family="Massarinaceae" genus="Massarina" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Massarina pandanicola" order="Pleosporales" pageId="14" pageNumber="39" phylum="Ascomycota" rank="species" species="pandanicola">Massarina pandanicola</taxonomicName>
(MFLU 18-0004, holotype). a Colony on MEA media b
<taxonomicName genus="Mycelium" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Mycelium" pageId="14" pageNumber="39" phylum="Basidiomycota" rank="genus">Mycelium</taxonomicName>
masses
<normalizedToken originalValue="cg">c-g</normalizedToken>
<taxonomicName class="Arthoniomycetes" family="Arthoniaceae" genus="Conidia" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Conidia" order="Arthoniales" pageId="14" pageNumber="39" phylum="Ascomycota" rank="genus">Conidia</taxonomicName>
and conidiogenous cells h
<taxonomicName class="Arthoniomycetes" family="Arthoniaceae" genus="Conidia" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Conidia" order="Arthoniales" pageId="14" pageNumber="39" phylum="Ascomycota" rank="genus">Conidia</taxonomicName>
. Scale bars: 20
<normalizedToken originalValue="μm">μm</normalizedToken>
(b), 2
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<normalizedToken originalValue="cg">c-g</normalizedToken>
), 5
<normalizedToken originalValue="μm">μm</normalizedToken>
(h).
</paragraph>
</caption>
</subSubSection>
</treatment>
</document>