117 lines
14 KiB
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117 lines
14 KiB
XML
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<mods:title id="28E650DCD5E785802AFD08DBDC8C05D0">Bioactive diterpenoid metabolism and cytotoxic activities of genetically transformed Euphorbia lathyris roots</mods:title>
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<mods:namePart id="A23F1A49C282AF8B220DD4DFC4B115CA">Ricigliano, Vincent A.</mods:namePart>
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<mods:affiliation id="E96E2DBB0FCB265694C670B9D6D91AEB">* & USDA-ARS, Honey Bee Breeding, Genetics and Physiology Research, Baton Rouge, LA, 70820, USA</mods:affiliation>
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<mods:namePart id="69AF3DBA7C71E799E264F3CAB9D302FD">Sica, Vincent P.</mods:namePart>
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<mods:namePart id="BA2908C90FEE747BADF1FA315789F3F2">Knowles, Sonja L.</mods:namePart>
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<mods:namePart id="E4603FCBFFF6D0E3DB0B4EF1286B3D17">Howarth, Dianella G.</mods:namePart>
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<mods:namePart id="66ABC6F63EDCD3624F66D454CB9C31C1">Oberlies, Nicholas H.</mods:namePart>
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<mods:date id="43A872299BB25883094F48F659E74F2E">2020</mods:date>
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<mods:title id="51595C135735976817D11B3424318438">112504</mods:title>
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<treatment id="D44487CF1842FF92FFE2F8E1F506FE7F" ID-DOI="http://doi.org/10.5281/zenodo.8301808" ID-Zenodo-Dep="8301808" LSID="urn:lsid:plazi:treatment:D44487CF1842FF92FFE2F8E1F506FE7F" httpUri="http://treatment.plazi.org/id/D44487CF1842FF92FFE2F8E1F506FE7F" lastPageNumber="2" pageId="1" pageNumber="2">
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<subSubSection id="14F765521842FF92FFE2F8E1F2F8F890" box="[100,754,1878,1898]" pageId="1" pageNumber="2" type="nomenclature">
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<heading id="071A81B51842FF92FFE2F8E1F2F8F890" bold="true" box="[100,754,1878,1898]" centered="true" fontSize="36" level="1" pageId="1" pageNumber="2" reason="1">
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<emphasis id="6E99EACB1842FF92FFE2F8E1F2F8F890" bold="true" box="[100,754,1878,1898]" italics="true" pageId="1" pageNumber="2">
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2.1. Genetic transformation and establishment of
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<taxonomicName id="9BED4D5A1842FF92FDA5F8E1F277F890" authority="L." box="[547,637,1878,1898]" class="Magnoliopsida" family="Euphorbiaceae" genus="Euphorbia" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="2" phylum="Tracheophyta" rank="subSpecies" species="lathyris" subSpecies="root">E. lathyris</taxonomicName>
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root cultures
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</emphasis>
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<subSubSection id="14F765521842FF92FF02F839F506FE7F" pageId="1" pageNumber="2" type="description">
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<paragraph id="5C5236D91842FF92FF02F839F506FE7F" blockId="1.[100,770,1934,1982]" lastBlockId="1.[818,1487,147,390]" pageId="1" pageNumber="2">
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Transformed roots of
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<taxonomicName id="9BED4D5A1842FF92FED3F839F1BBF858" box="[341,433,1934,1954]" class="Magnoliopsida" family="Euphorbiaceae" genus="Euphorbia" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="2" phylum="Tracheophyta" rank="species" species="lathyris">
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<emphasis id="6E99EACB1842FF92FED3F839F1BBF858" bold="true" box="[341,433,1934,1954]" italics="true" pageId="1" pageNumber="2">E. lathyris</emphasis>
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</taxonomicName>
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were established via
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<taxonomicName id="9BED4D5A1842FF92FD05F839F2F1F858" authorityName="Conn" authorityYear="1942" baseAuthorityName="Riker et al." baseAuthorityYear="1930" box="[643,763,1934,1954]" class="Alphaproteobacteria" family="Rhizobiaceae" genus="Agrobacterium" kingdom="Bacteria" order="Rhizobiales" pageId="1" pageNumber="2" phylum="Proteobacteria" rank="species" species="rhizogenes">
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<emphasis id="6E99EACB1842FF92FD05F839F2F1F858" bold="true" box="[643,763,1934,1954]" italics="true" pageId="1" pageNumber="2">A. rhizogenes</emphasis>
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</taxonomicName>
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- mediated infection of greenhouse grown plants. Stem explants of
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<taxonomicName id="9BED4D5A1842FF92FCB4FF22F381FF5F" box="[818,907,147,167]" class="Magnoliopsida" family="Euphorbiaceae" genus="Euphorbia" kingdom="Plantae" order="Malpighiales" pageId="1" pageNumber="2" phylum="Tracheophyta" rank="species" species="lathyris">
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<emphasis id="6E99EACB1842FF92FCB4FF22F381FF5F" bold="true" box="[818,907,147,167]" italics="true" pageId="1" pageNumber="2">E. lathyris</emphasis>
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</taxonomicName>
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were used as the target tissue for infection and root induction (
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<figureCitation id="C4D62A5C1842FF92FCBCFF06F371FF3A" box="[826,891,176,195]" captionStart="Fig" captionStartId="2.[100,130,773,790]" captionTargetBox="[341,1249,149,745]" captionTargetId="figure-7@2.[338,1250,148,746]" captionTargetPageId="2" captionText="Fig. 1. Establishment and culture maintenance of transformed E. lathyris roots. (a) Stem explants of 3-week-old greenhouse grown plants were used for co-culture with A. rhizogenes. (b) Roots emerged from callus at the site of infection after 2–3 weeks.(c) Adventitious roots displaying the characteristic of the “hairy root” phenotype. (d) Growth characteristics of the isogenic root line used in this study on agar media and in (e) liquid media." figureDoi="http://doi.org/10.5281/zenodo.8293458" httpUri="https://zenodo.org/record/8293458/files/figure.png" pageId="1" pageNumber="2">Fig. 1a</figureCitation>
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). Root primordia were observed at the infection sites, emerging from callus as early as 15 days after inoculation (
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<figureCitation id="C4D62A5C1842FF92FA8FFF7DF547FF27" box="[1289,1357,203,223]" captionStart="Fig" captionStartId="2.[100,130,773,790]" captionTargetBox="[341,1249,149,745]" captionTargetId="figure-7@2.[338,1250,148,746]" captionTargetPageId="2" captionText="Fig. 1. Establishment and culture maintenance of transformed E. lathyris roots. (a) Stem explants of 3-week-old greenhouse grown plants were used for co-culture with A. rhizogenes. (b) Roots emerged from callus at the site of infection after 2–3 weeks.(c) Adventitious roots displaying the characteristic of the “hairy root” phenotype. (d) Growth characteristics of the isogenic root line used in this study on agar media and in (e) liquid media." figureDoi="http://doi.org/10.5281/zenodo.8293458" httpUri="https://zenodo.org/record/8293458/files/figure.png" pageId="1" pageNumber="2">Fig. 1b</figureCitation>
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). Fully developed adventitious roots showed an abundance of root hairs along the axis that are characteristic of the “hairy root” phenotype (
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<figureCitation id="C4D62A5C1842FF92FAD9FEB5F5A8FEEF" box="[1375,1442,259,278]" captionStart="Fig" captionStartId="2.[100,130,773,790]" captionTargetBox="[341,1249,149,745]" captionTargetId="figure-7@2.[338,1250,148,746]" captionTargetPageId="2" captionText="Fig. 1. Establishment and culture maintenance of transformed E. lathyris roots. (a) Stem explants of 3-week-old greenhouse grown plants were used for co-culture with A. rhizogenes. (b) Roots emerged from callus at the site of infection after 2–3 weeks.(c) Adventitious roots displaying the characteristic of the “hairy root” phenotype. (d) Growth characteristics of the isogenic root line used in this study on agar media and in (e) liquid media." figureDoi="http://doi.org/10.5281/zenodo.8293458" httpUri="https://zenodo.org/record/8293458/files/figure.png" pageId="1" pageNumber="2">Fig. 1c</figureCitation>
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). An isogenic root line displaying vigorous growth was propagated on hormone-free solid media (
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<figureCitation id="C4D62A5C1842FF92FBBEFE8DF477FEB7" box="[1080,1149,315,334]" captionStart="Fig" captionStartId="2.[100,130,773,790]" captionTargetBox="[341,1249,149,745]" captionTargetId="figure-7@2.[338,1250,148,746]" captionTargetPageId="2" captionText="Fig. 1. Establishment and culture maintenance of transformed E. lathyris roots. (a) Stem explants of 3-week-old greenhouse grown plants were used for co-culture with A. rhizogenes. (b) Roots emerged from callus at the site of infection after 2–3 weeks.(c) Adventitious roots displaying the characteristic of the “hairy root” phenotype. (d) Growth characteristics of the isogenic root line used in this study on agar media and in (e) liquid media." figureDoi="http://doi.org/10.5281/zenodo.8293458" httpUri="https://zenodo.org/record/8293458/files/figure.png" pageId="1" pageNumber="2">Fig. 1d</figureCitation>
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), and then grown in quadruplicate liquid culture flasks on an orbital shaker to generate the experimental biomass used in subsequent experiments (
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<figureCitation id="C4D62A5C1842FF92FB38FEC5F50AFE7F" box="[1214,1280,371,390]" captionStart="Fig" captionStartId="2.[100,130,773,790]" captionTargetBox="[341,1249,149,745]" captionTargetId="figure-7@2.[338,1250,148,746]" captionTargetPageId="2" captionText="Fig. 1. Establishment and culture maintenance of transformed E. lathyris roots. (a) Stem explants of 3-week-old greenhouse grown plants were used for co-culture with A. rhizogenes. (b) Roots emerged from callus at the site of infection after 2–3 weeks.(c) Adventitious roots displaying the characteristic of the “hairy root” phenotype. (d) Growth characteristics of the isogenic root line used in this study on agar media and in (e) liquid media." figureDoi="http://doi.org/10.5281/zenodo.8293458" httpUri="https://zenodo.org/record/8293458/files/figure.png" pageId="1" pageNumber="2">Fig. 1e</figureCitation>
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).
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |