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<document id="789A18A206A13F02DD6FBDA97B7134CC" ID-CLB-Dataset="300899" ID-DOI="10.37828/em.2023.70.3" ID-GBIF-Dataset="dcdc9b21-8cf1-4aa1-b710-6eaa81712a69" ID-ISSN="2336-9744" ID-Zenodo-Dep="13247199" ID-ZooBank="urn:lsid:zoobank.org:pub:B3184D3B-12AA-4BDA-9BB3-6671E5AD10BC" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1722972582719" checkinUser="felipe" docAuthor="Pešić, Vladimir, Zawal, Andrzej, Gülle, Pinar, Cakmak, Ibrahim &amp; Saboori, Alireza" docDate="2023" docId="88018425943CFFD0FF27BFDBFC63FD68" docLanguage="en" docName="EcolMontenegrina.70.14-23.pdf" docOrigin="Ecologica Montenegrina 70" docSource="http://dx.doi.org/10.37828/em.2023.70.3" docStyle="DocumentStyle:3485E46B0EDC64DBF2992EDE30BFB5AE.2:EcolMontenegrina.2017-.journal_article" docStyleId="3485E46B0EDC64DBF2992EDE30BFB5AE" docStyleName="EcolMontenegrina.2017-.journal_article" docStyleVersion="2" docTitle="Hygrobates (Hygrobates) ponticus subsp. yildirimi Pešić, Zawal, Gülle, Cakmak &amp; Saboori, 2023, subsp. nov." docType="treatment" docUuid="B5642966-444E-4257-933F-72DCCB40D589" docUuidSource="ZooBank" docVersion="4" lastPageNumber="22" masterDocId="7438FC5D943FFFD8FFB0BA58FFB3FFFD" masterDocTitle="Integrative description of a new subspecies of the water mite genus Hygrobates Koch, 1837 from southwestern Türkiye (Acariformes, Hydrachnidia, Hygrobatidae)" masterLastPageNumber="23" masterPageNumber="14" pageNumber="17" updateTime="1722982451465" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:title id="0F21E35E37693BA2D74ED3EEEF00521A">Integrative description of a new subspecies of the water mite genus Hygrobates Koch, 1837 from southwestern Türkiye (Acariformes, Hydrachnidia, Hygrobatidae)</mods:title>
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<mods:namePart id="6807EE58FCC7F5E5D708F817068FE956">Pešić, Vladimir</mods:namePart>
<mods:affiliation id="12E80FBBC88F556BD6133D79BB0CBF22">Department of Biology, University of Montenegro, Cetinjski put b. b., 81000 Podgorica, Montenegro.</mods:affiliation>
<mods:nameIdentifier id="3E58284B184D5ECEABF1263ECB14AFB5" type="email">vladopesic@gmail.com</mods:nameIdentifier>
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<mods:namePart id="D996BD2F9580D8BE3A251B7EDA84A712">Zawal, Andrzej</mods:namePart>
<mods:nameIdentifier id="2A6E8ECE07B9F3FA11531E1771BD598D" type="ORCID">0000-0002-5838-6060&amp;0000-0002-3101-1330</mods:nameIdentifier>
<mods:affiliation id="3C2AE411B71B87235900346BDD48BD8B">Institute of Marine and Environmental Sciences, Center of Molecular Biology and Biotechnology, University of Szczecin, Wąska 13, 71 - 415 Szczecin, Poland. E-mail: andrzej. zawal @ usz. edu. pl, https: // orcid. org / 0000 - 0002 - 5838 - 6060 &amp; Department of Plant Protection, Faculty of Agriculture, University of Tehran, Karaj, Iran. E-mail: saboori @ ut. ac. ir, https: // orcid. org / 0000 - 0002 - 3101 - 1330</mods:affiliation>
</mods:name>
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<mods:namePart id="E0B4011CE1088A7B7DF4FAB724DB38EB">Gülle, Pinar</mods:namePart>
<mods:nameIdentifier id="0C6B771C493F48AE547ABF1BFDA6C1D4" type="ORCID">0000-0003-1347-4283</mods:nameIdentifier>
<mods:affiliation id="3E5F6E867C5442531ACE05F4D21B215E">Mehmet Akif Ersoy University, Faculty of Science and Arts, Burdur, Türkiye. E-mail: pnarozsimsek @ gmail. com; https: // orcid. org / 0000 - 0003 - 1347 - 4283</mods:affiliation>
</mods:name>
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<mods:namePart id="89369021CD6F0353EA18B6E8727A0906">Cakmak, Ibrahim</mods:namePart>
<mods:nameIdentifier id="EE594F93663530DB9178CFABA9A30E91" type="ORCID">0000-0003-1903-3913</mods:nameIdentifier>
<mods:affiliation id="86964489C9B381421FABB6C3A69F1B5E">Department of Plant Protection, Faculty of Agriculture, Aydin Adnan Menderes University, Aydin, Türkiye. E-mail: icakmak @ adu. edu. tr; https: // orcid. org / 0000 - 0003 - 1903 - 3913 &amp; Department of Research and Innovation, Saveetha School of Engineering, SIMATS, Thandalam, Chennai, Tamil Nadu 602105, India. * Corresponding author. E-mail: vladopesic @ gmail. com; https: // orcid. org / 0000 - 0002 - 9724 - 345 X</mods:affiliation>
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<mods:namePart id="20B04C0F11C8C9ED98E2D3C9B4BFD5AF">Saboori, Alireza</mods:namePart>
<mods:nameIdentifier id="D344B38DE407E8BF372872801956C464" type="ORCID">0000-0002-3101-1330&amp;0000-0003-1903-3913</mods:nameIdentifier>
<mods:affiliation id="25E02476010C96E6F214C59412E9FCA0">Department of Plant Protection, Faculty of Agriculture, University of Tehran, Karaj, Iran. E-mail: saboori @ ut. ac. ir, https: // orcid. org / 0000 - 0002 - 3101 - 1330 &amp; Department of Plant Protection, Faculty of Agriculture, Aydin Adnan Menderes University, Aydin, Türkiye. E-mail: icakmak @ adu. edu. tr; https: // orcid. org / 0000 - 0003 - 1903 - 3913</mods:affiliation>
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<mods:title id="5217512A46DA9B3CA31D83C70348684B">Ecologica Montenegrina</mods:title>
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<mods:date id="1CF3F465F26E471AE5CC75790C0438C8">2023</mods:date>
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<mods:number id="12B94B57E152C3F42C7629EF440E9CB5">2023-11-24</mods:number>
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<mods:number id="C01868A459182C4C90B721428742917A">70</mods:number>
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<subSubSection id="48B266B8943CFFDBFF27BFDBFBDAFA46" pageId="3" pageNumber="17" type="nomenclature">
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<taxonomicName id="C7A84EB0943CFFDBFF27BFDBFD10FA60" authorityName="Pešić &amp; Zawal &amp; Gülle &amp; Cakmak &amp; Saboori" authorityYear="2023" box="[151,675,1411,1437]" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="3" pageNumber="17" phylum="Arthropoda" rank="subSpecies" species="ponticus" status="subsp. nov." subGenus="Hygrobates" subSpecies="yildirimi">
<emphasis id="32DCE921943CFFDBFF27BFDBFE91FA60" bold="true" box="[151,290,1411,1437]" italics="true" pageId="3" pageNumber="17">Hygrobates</emphasis>
(
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)
<emphasis id="32DCE921943CFFDBFE7EBFDBFD10FA60" bold="true" box="[462,675,1411,1437]" italics="true" pageId="3" pageNumber="17">ponticus yildirimi</emphasis>
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<taxonomicNameLabel id="29EF545A943CFFDBFD1ABFDBFC86FA60" box="[682,821,1411,1437]" pageId="3" pageNumber="17" rank="subSpecies">subsp. nov.</taxonomicNameLabel>
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https://zoobank.org/
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urn:lsid:zoobank.org:act:
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<subSubSection id="48B266B8943CFFDBFF27BF9BFF4CFA20" box="[151,255,1475,1501]" pageId="3" pageNumber="17" type="description">
<paragraph id="00173533943CFFDBFF27BF9BFF4CFA20" blockId="3.[151,1129,1411,1501]" box="[151,255,1475,1501]" pageId="3" pageNumber="17">
<figureCitation id="989329B6943CFFDBFF27BF9BFF4CFA20" box="[151,255,1475,1501]" captionStart-0="Figure 1" captionStart-1="Figure 2" captionStartId-0="4.[151,227,1967,1990]" captionStartId-1="5.[151,227,1759,1782]" captionTargetBox-0="[273,1303,190,1942]" captionTargetBox-1="[168,1419,195,1724]" captionTargetId-0="figure-8@4.[260,1327,189,1948]" captionTargetId-1="figure-10@5.[160,1427,189,1741]" captionTargetPageId-0="4" captionTargetPageId-1="5" captionText-0="Figure 1. Hygrobates ponticus yildirimi ssp. nov. (A ♀ holotype CCDB_44300_E09; B ♂ paratype CCDB_39399_B04). A coxal and genital field; B genital field. Scale bars = 100 μm." captionText-1="Figure 2. Hygrobates ponticus yildirimi subsp. nov. (A-C ♂ paratype CCDB_39399_B04; B ♀ holotype CCDB_44300_E09). A palp, medial view; B palp, lateral view (P-1 lacking); C I-L-5 and -6; D palp, lateral view. Scale bar = 100 μm." figureDoi-0="http://doi.org/10.5281/zenodo.13247201" figureDoi-1="http://doi.org/10.5281/zenodo.13247203" httpUri-0="https://zenodo.org/record/13247201/files/figure.png" httpUri-1="https://zenodo.org/record/13247203/files/figure.png" pageId="3" pageNumber="17">Figs. 1-2</figureCitation>
</paragraph>
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<emphasis id="32DCE921943CFFDBFF27BC5EFE3AF9DD" bold="true" box="[151,393,1542,1568]" pageId="3" pageNumber="17">Material examined</emphasis>
<materialsCitation id="B0C03F6E943CFFDBFE0DBC5EFDD1F999" ID-GBIF-Occurrence="4912542302" collectingDate="2022-08-21" collectionCode="RMNH" collectorName="Gulle." country="Turkey" county="Aksu" elevation="1187" latitude="37.7454" location="Aksu Stream-Koprucay River" longLatPrecision="6" longitude="31.0275" municipality="Pazarkoy" pageId="3" pageNumber="17" specimenCode="HYDOC057-22" specimenCount="1" specimenCount-female="1" stateProvince="Isparta" typeStatus="holotype">
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, sequenced (
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), dissected and slide mounted (
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),
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,
<collectingRegion id="C26CFBD1943CFFDBFE98BC70FE58F9BF" box="[296,491,1576,1602]" country="Turkey" name="Isparta" pageId="3" pageNumber="17">Isparta Province</collectingRegion>
,
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,
<collectingMunicipality id="E073AF49943CFFDBFDF6BC70FD05F9BF" box="[582,694,1576,1602]" pageId="3" pageNumber="17">Pazarköy</collectingMunicipality>
,
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,
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,
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,
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<quantity id="C75098D6943CFFDBFAD2BC70FF1DF999" metricMagnitude="3" metricUnit="m" metricValue="1.187" pageId="3" pageNumber="17" unit="m" value="1187.0">1187 m</quantity>
asl
</elevation>
.,
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<collectingDate id="6452EA1B943CFFDBFF4CBC12FE7CF999" box="[252,463,1610,1636]" pageId="3" pageNumber="17" value="2022-08-21">21. August 2022</collectingDate>
</date>
leg.
<collectorName id="AD5D50E5943CFFDBFDA9BC12FDD1F999" box="[537,610,1610,1636]" pageId="3" pageNumber="17">Gülle.</collectorName>
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<materialsCitation id="B0C03F6E943CFFDBFDC1BC12FED9F97B" ID-GBIF-Occurrence="4912542306" collectingDate="2022-08-21" collectionCode="HYDOC" collectorName="Gulle." country="Turkey" county="Aksu" elevation="1187" latitude="37.7454" location="Aksu Stream-Koprucay River" longLatPrecision="6" longitude="31.0275" municipality="Pazarkoy" pageId="3" pageNumber="17" specimenCode="HYDOC059-22" specimenCount="1" specimenCount-female="1" stateProvince="Isparta" typeStatus="paratype">
<typeStatus id="DF138B91943CFFDBFDC1BC12FD5BF999" box="[625,744,1610,1636]" pageId="3" pageNumber="17" type="paratype">Paratypes</typeStatus>
:
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, same place and data as the holotype, sequenced (
<specimenCode id="500E9D48943CFFDBFF2FBC34FED1F97B" box="[159,354,1644,1670]" collectionCode="HYDOC" pageId="3" pageNumber="17">HYDOC059-22</specimenCode>
)
</materialsCitation>
;
<materialsCitation id="B0C03F6E943CFFDBFECFBC34FCBEF97B" ID-GBIF-Occurrence="4912542305" box="[383,781,1644,1670]" collectionCode="HYDIR" country="Turkey" pageId="3" pageNumber="17" specimenCode="HYDIR016-23" specimenCount="1" specimenCount-male="1" stateProvince="Isparta" typeStatus="paratype">
<specimenCount id="16AEFEBA943CFFDBFECFBC34FE16F97B" box="[383,421,1644,1670]" count="1" pageId="3" pageNumber="17" type="male">1♂</specimenCount>
, sequenced (
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)
</materialsCitation>
,
<materialsCitation id="B0C03F6E943CFFDBFC93BC34FB63F95A" ID-GBIF-Occurrence="4912542301" collectingDate="2023-03-14" collectorName="Pesic" country="Turkey" latitude="37.9297" location="Sirindere" longLatPrecision="6" longitude="27.7775" pageId="3" pageNumber="17" specimenCount="1" stateProvince="Aydin" typeStatus="paratype">
<collectingRegion id="C26CFBD1943CFFDBFC93BC34FC5BF97B" box="[803,1000,1644,1670]" country="Turkey" name="Aydin" pageId="3" pageNumber="17">Aydin Province</collectingRegion>
, TR6-2023 stream near
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,
<geoCoordinate id="659C53F4943CFFDBFF27BCD5FE9FF95A" box="[151,300,1677,1703]" degrees="37.9297" direction="north" orientation="latitude" pageId="3" pageNumber="17" precision="5" value="37.9297">37.9297° N</geoCoordinate>
,
<geoCoordinate id="659C53F4943CFFDBFEF1BCD5FE61F95A" box="[321,466,1677,1703]" degrees="27.7775" direction="east" orientation="longitude" pageId="3" pageNumber="17" precision="5" value="27.7775">27.7775° E</geoCoordinate>
,
<date id="741613F3943CFFDBFE58BCD5FD08F95A" box="[488,699,1677,1703]" pageId="3" pageNumber="17" value="2023-03-14">
<collectingDate id="6452EA1B943CFFDBFE58BCD5FD08F95A" box="[488,699,1677,1703]" pageId="3" pageNumber="17" value="2023-03-14">14. March 2023</collectingDate>
</date>
, leg.
<collectorName id="AD5D50E5943CFFDBFCBCBCD5FCFEF95A" box="[780,845,1677,1703]" pageId="3" pageNumber="17">Pešić</collectorName>
, dissected and slide mounted
</materialsCitation>
;
<materialsCitation id="B0C03F6E943CFFDBFB55BCD5FEEEF934" ID-GBIF-Occurrence="4912542303" collectionCode="HYDIR" country="Turkey" pageId="3" pageNumber="17" specimenCode="HYDIR030-23" specimenCount="1" specimenCount-female="1" stateProvince="Aydin" typeStatus="paratype">
<specimenCount id="16AEFEBA943CFFDBFB55BCD5FAB9F95A" box="[1253,1290,1677,1703]" count="1" pageId="3" pageNumber="17" type="female">1♀</specimenCount>
, sequenced (
<specimenCode id="500E9D48943CFFDBFF2FBCF7FEE5F934" box="[159,342,1711,1737]" collectionCode="HYDIR" pageId="3" pageNumber="17">HYDIR030-23</specimenCode>
)
</materialsCitation>
,
<materialsCitation id="B0C03F6E943CFFDBFEDDBCF7FDDFF916" ID-GBIF-Occurrence="4912542304" collectingDate="2023-03-18" collectionCode="RMNH" collectorName="Pesic" country="Turkey" latitude="37.8744" location="Kalfakoy" longLatPrecision="6" longitude="27.8459" municipality="Tabakhane" pageId="3" pageNumber="17" specimenCount="1" stateProvince="Aydin" typeStatus="paratype">
<collectingRegion id="C26CFBD1943CFFDBFEDDBCF7FD9EF934" box="[365,557,1711,1737]" country="Turkey" name="Aydin" pageId="3" pageNumber="17">Aydin Province</collectingRegion>
, TR12-2023
<collectingMunicipality id="E073AF49943CFFDBFD7EBCF7FCFCF934" box="[718,847,1711,1737]" pageId="3" pageNumber="17">Tabakhane</collectingMunicipality>
stream near
<location id="057763E8943CFFDBFC40BCF7FBD3F934" LSID="urn:lsid:plazi:treatment:88018425943CFFD0FF27BFDBFC63FD68:057763E8943CFFDBFC40BCF7FBD3F934" box="[1008,1120,1711,1737]" country="Turkey" latitude="37.8744" longLatPrecision="6" longitude="27.8459" municipality="Tabakhane" name="Kalfakoy" pageId="3" pageNumber="17" stateProvince="Aydin">Kalfaköy</location>
,
<geoCoordinate id="659C53F4943CFFDBFBC0BCF7FB4DF934" box="[1136,1278,1711,1737]" degrees="37.8744" direction="north" orientation="latitude" pageId="3" pageNumber="17" precision="5" value="37.8744">37.8744° N</geoCoordinate>
,
<geoCoordinate id="659C53F4943CFFDBFABDBCF7FA24F934" box="[1293,1431,1711,1737]" degrees="27.8459" direction="east" orientation="longitude" pageId="3" pageNumber="17" precision="5" value="27.8459">27.8459° E</geoCoordinate>
,
<date id="741613F3943CFFDBFF27BC89FEE7F916" box="[151,340,1745,1771]" pageId="3" pageNumber="17" value="2023-03-18">
<collectingDate id="6452EA1B943CFFDBFF27BC89FEE7F916" box="[151,340,1745,1771]" pageId="3" pageNumber="17" value="2023-03-18">18. March 2023</collectingDate>
</date>
, leg.
<collectorName id="AD5D50E5943CFFDBFE23BC89FE62F916" box="[403,465,1745,1771]" pageId="3" pageNumber="17">Pešić</collectorName>
(all
<collectionCode id="66B9ADF6943CFFDBFDB7BC89FDD6F916" box="[519,613,1745,1771]" country="Netherlands" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34992" lsid="urn:lsid:biocol.org:col:34992" name="National Museum of Natural History, Naturalis" pageId="3" pageNumber="17" type="Museum">RMNH</collectionCode>
)
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="48B266B8943CFFDBFF27BD4CFC3CF848" pageId="3" pageNumber="17" type="diagnosis">
<paragraph id="00173533943CFFDBFF27BD4CFC3CF848" blockId="3.[151,1437,1812,1973]" pageId="3" pageNumber="17">
<emphasis id="32DCE921943CFFDBFF27BD4CFEA3F8D3" bold="true" box="[151,272,1812,1838]" pageId="3" pageNumber="17">Diagnosis</emphasis>
<emphasis id="32DCE921943CFFDBFE8FBD4CFE5CF8D3" box="[319,495,1812,1838]" italics="true" pageId="3" pageNumber="17">Morphological</emphasis>
: P-2 with a triangular, bluntly pointed, moderately elongated (L/W ratio about 1.2) ventrodistal projection. Anterior margin of the male genital field with a shallow indentation and a small knob-shaped medial projection in the centre of the indentation; Ac-2 and Ac-3 placed next to each other; female gonopore longer than the genital plates.
<emphasis id="32DCE921943CFFDBFD52BD21FCEFF86E" box="[738,860,1913,1939]" italics="true" pageId="3" pageNumber="17">Molecular</emphasis>
: this lineage differs from
<taxonomicName id="C7A84EB0943CFFDBFB12BD21FA2FF86E" authorityName="Pesic, Esen &amp; Mumladze" authorityYear="2022" box="[1186,1436,1913,1939]" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="3" pageNumber="17" phylum="Arthropoda" rank="subSpecies" species="ponticus" subSpecies="ponticus">
<emphasis id="32DCE921943CFFDBFB12BD21FA2FF86E" box="[1186,1436,1913,1939]" italics="true" pageId="3" pageNumber="17">H. ponticus ponticus</emphasis>
</taxonomicName>
clade from
<collectingCountry id="78BF75A3943CFFDBFEACBDC3FEC8F848" box="[284,379,1947,1973]" name="Georgia" pageId="3" pageNumber="17">Georgia</collectingCountry>
and Eastern
<collectingCountry id="78BF75A3943CFFDBFDA3BDC3FDC3F848" box="[531,624,1947,1973]" name="Turkey" pageId="3" pageNumber="17">Türkiye</collectingCountry>
by 10.2% K2P for COI.
</paragraph>
</subSubSection>
<caption id="54D765BB943BFFDCFF27BDF7FBF6F818" ID-DOI="http://doi.org/10.5281/zenodo.13247201" ID-Zenodo-Dep="13247201" httpUri="https://zenodo.org/record/13247201/files/figure.png" pageId="4" pageNumber="18" startId="4.[151,227,1967,1990]" targetBox="[273,1303,190,1942]" targetPageId="4" targetType="figure">
<paragraph id="00173533943BFFDCFF27BDF7FBF6F818" blockId="4.[151,1436,1967,2021]" pageId="4" pageNumber="18">
<emphasis id="32DCE921943BFFDCFF27BDF7FEB1F83B" bold="true" box="[151,258,1967,1990]" pageId="4" pageNumber="18">Figure 1</emphasis>
.
<taxonomicName id="C7A84EB0943BFFDCFEACBDF7FDC0F83B" authorityName="Pešić &amp; Zawal &amp; Gülle &amp; Cakmak &amp; Saboori" authorityYear="2023" box="[284,627,1967,1990]" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="4" pageNumber="18" phylum="Arthropoda" rank="subSpecies" species="ponticus" status="subsp. nov." subSpecies="yildirimi">
<emphasis id="32DCE921943BFFDCFEACBDF7FDC0F83B" box="[284,627,1967,1990]" italics="true" pageId="4" pageNumber="18">Hygrobates ponticus yildirimi</emphasis>
</taxonomicName>
<taxonomicNameLabel id="29EF545A943BFFDCFD36BDF7FD40F83B" box="[646,755,1967,1990]" pageId="4" pageNumber="18" rank="subSpecies">
<emphasis id="32DCE921943BFFDCFD36BDF7FD5EF83B" bold="true" box="[646,749,1967,1990]" pageId="4" pageNumber="18">ssp. nov</emphasis>
.
</taxonomicNameLabel>
(A ♀ holotype CCDB_44300_E09; B ♂ paratype CCDB_39399_B04). A coxal and genital field; B genital field. Scale bars = 100 μm.
</paragraph>
</caption>
<caption id="54D765BB943AFFDDFF27BC87FE03F8C9" ID-DOI="http://doi.org/10.5281/zenodo.13247203" ID-Zenodo-Dep="13247203" httpUri="https://zenodo.org/record/13247203/files/figure.png" pageId="5" pageNumber="19" startId="5.[151,227,1759,1782]" targetBox="[168,1419,195,1724]" targetPageId="5" targetType="figure">
<paragraph id="00173533943AFFDDFF27BC87FE03F8C9" blockId="5.[151,1436,1759,1844]" pageId="5" pageNumber="19">
<emphasis id="32DCE921943AFFDDFF27BC87FF4DF90B" bold="true" box="[151,254,1759,1782]" pageId="5" pageNumber="19">Figure 2</emphasis>
.
<taxonomicName id="C7A84EB0943AFFDDFEA4BC87FDD0F90B" authorityName="Pešić &amp; Zawal &amp; Gülle &amp; Cakmak &amp; Saboori" authorityYear="2023" box="[276,611,1759,1782]" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="5" pageNumber="19" phylum="Arthropoda" rank="subSpecies" species="ponticus" status="subsp. nov." subSpecies="yildirimi">
<emphasis id="32DCE921943AFFDDFEA4BC87FDD0F90B" box="[276,611,1759,1782]" italics="true" pageId="5" pageNumber="19">Hygrobates ponticus yildirimi</emphasis>
</taxonomicName>
<taxonomicNameLabel id="29EF545A943AFFDDFDC3BC87FD49F90B" box="[627,762,1759,1782]" pageId="5" pageNumber="19" rank="subSpecies">
<emphasis id="32DCE921943AFFDDFDC3BC87FD41F90B" bold="true" box="[627,754,1759,1782]" pageId="5" pageNumber="19">subsp. nov</emphasis>
.
</taxonomicNameLabel>
(A-C ♂ paratype CCDB_39399_B04; B ♀ holotype CCDB_44300_E09). A palp, medial view; B palp, lateral view (P-1 lacking); C I-L-5 and -6; D palp, lateral view. Scale bar = 100 μm.
</paragraph>
</caption>
<subSubSection id="48B266B8943AFFDFFF27BDD8FAA9FD68" lastPageId="7" lastPageNumber="21" pageId="5" pageNumber="19" type="description">
<paragraph id="00173533943AFFDEFF27BDD8FD7DFE55" blockId="5.[151,1437,1920,2047]" lastBlockId="6.[151,1437,196,424]" lastPageId="6" lastPageNumber="20" pageId="5" pageNumber="19">
<emphasis id="32DCE921943AFFDDFF27BDD8FE9BF867" bold="true" box="[151,296,1920,1946]" pageId="5" pageNumber="19">Description</emphasis>
.
<emphasis id="32DCE921943AFFDDFE88BDD8FE4DF867" box="[312,510,1920,1946]" italics="true" pageId="5" pageNumber="19">General features</emphasis>
— Colour yellow to brown, integument soft, strongly lineated. Posteromedial margin of Cx-I+II obtuse-triangular, caudo-lateral apodemes of Cx-I+II moderately developed; Cx-IV subtriangular, with a distinct nose-like protruding medial margin (
<figureCitation id="989329B6943AFFDDFC6CBD9BFBD3F820" box="[988,1120,1987,2013]" captionStart="Figure 1" captionStartId="4.[151,227,1967,1990]" captionTargetBox="[273,1303,190,1942]" captionTargetId="figure-8@4.[260,1327,189,1948]" captionTargetPageId="4" captionText="Figure 1. Hygrobates ponticus yildirimi ssp. nov. (A ♀ holotype CCDB_44300_E09; B ♂ paratype CCDB_39399_B04). A coxal and genital field; B genital field. Scale bars = 100 μm." figureDoi="http://doi.org/10.5281/zenodo.13247201" httpUri="https://zenodo.org/record/13247201/files/figure.png" pageId="5" pageNumber="19">Figure 1A</figureCitation>
). Acetabula in triangular arrangement. P-2 ventral margin distally forming a triangular, bluntly pointed projection covered by small, scattered denticles; P-3 with denticles covering distal two-thirds of ventral margin; P-4 ventral setae separated (
<figureCitation id="989329B69439FFDEFEA5BABEFE36FEFD" box="[277,389,230,256]" captionStart="Figure 2" captionStartId="5.[151,227,1759,1782]" captionTargetBox="[168,1419,195,1724]" captionTargetId="figure-10@5.[160,1427,189,1741]" captionTargetPageId="5" captionText="Figure 2. Hygrobates ponticus yildirimi subsp. nov. (A-C ♂ paratype CCDB_39399_B04; B ♀ holotype CCDB_44300_E09). A palp, medial view; B palp, lateral view (P-1 lacking); C I-L-5 and -6; D palp, lateral view. Scale bar = 100 μm." figureDoi="http://doi.org/10.5281/zenodo.13247203" httpUri="https://zenodo.org/record/13247203/files/figure.png" pageId="6" pageNumber="20">Figures 2</figureCitation>
A-B, D).
<emphasis id="32DCE9219439FFDEFE44BABEFD83FEFD" box="[500,560,230,256]" italics="true" pageId="6" pageNumber="20">Male</emphasis>
— Anterior margin of genital field with a shallow indentation and a small knob-shaped medial projection in the centre of indentation, genital plate typically with an irregular margin of secondary sclerotization, posterior margin of genital field deeply indented with a small protrusion in the centre of the indentation, not extending beyond the posterior margin, Ac-3 subtriangular but not strongly elongated (
<figureCitation id="989329B69439FFDEFEACBB35FE2AFE7A" box="[284,409,365,391]" captionStart="Figure 1" captionStartId="4.[151,227,1967,1990]" captionTargetBox="[273,1303,190,1942]" captionTargetId="figure-8@4.[260,1327,189,1948]" captionTargetPageId="4" captionText="Figure 1. Hygrobates ponticus yildirimi ssp. nov. (A ♀ holotype CCDB_44300_E09; B ♂ paratype CCDB_39399_B04). A coxal and genital field; B genital field. Scale bars = 100 μm." figureDoi="http://doi.org/10.5281/zenodo.13247201" httpUri="https://zenodo.org/record/13247201/files/figure.png" pageId="6" pageNumber="20">Figure 1B</figureCitation>
).
<emphasis id="32DCE9219439FFDEFE03BB35FDB8FE7A" box="[435,523,365,391]" italics="true" pageId="6" pageNumber="20">Female</emphasis>
— Genital plates with a narrow border of porose secondary sclerotization, gonopore longer than genital plates (
<figureCitation id="989329B69439FFDEFDF4BBD6FD73FE55" box="[580,704,398,424]" captionStart="Figure 1" captionStartId="4.[151,227,1967,1990]" captionTargetBox="[273,1303,190,1942]" captionTargetId="figure-8@4.[260,1327,189,1948]" captionTargetPageId="4" captionText="Figure 1. Hygrobates ponticus yildirimi ssp. nov. (A ♀ holotype CCDB_44300_E09; B ♂ paratype CCDB_39399_B04). A coxal and genital field; B genital field. Scale bars = 100 μm." figureDoi="http://doi.org/10.5281/zenodo.13247201" httpUri="https://zenodo.org/record/13247201/files/figure.png" pageId="6" pageNumber="20">Figure 1A</figureCitation>
).
</paragraph>
<caption id="54D765BB9439FFDEFF27BD8AFAD2F814" ID-DOI="http://doi.org/10.5281/zenodo.13247205" ID-Zenodo-Dep="13247205" box="[151,1377,2002,2025]" httpUri="https://zenodo.org/record/13247205/files/figure.png" pageId="6" pageNumber="20" startId="6.[151,227,2002,2025]" targetBox="[164,1434,466,1952]" targetPageId="6" targetType="figure">
<paragraph id="001735339439FFDEFF27BD8AFAD2F814" blockId="6.[151,1377,2002,2025]" box="[151,1377,2002,2025]" pageId="6" pageNumber="20">
<emphasis id="32DCE9219439FFDEFF27BD8AFF4EF814" bold="true" box="[151,253,2002,2025]" pageId="6" pageNumber="20">Figure 3.</emphasis>
Neighbour-joining tree of the
<taxonomicName id="C7A84EB09439FFDEFDF4BD8AFCA9F814" authorityName="Piersig" authorityYear="1896" box="[580,794,2002,2025]" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="6" pageNumber="20" phylum="Arthropoda" rank="species" species="calliger">
<emphasis id="32DCE9219439FFDEFDF4BD8AFCA9F814" box="[580,794,2002,2025]" italics="true" pageId="6" pageNumber="20">Hygrobates calliger</emphasis>
</taxonomicName>
-complex obtained from 47 nucleotide COI sequences.
</paragraph>
</caption>
<paragraph id="001735339438FFDFFF45BA9CFDA4FEBE" blockId="7.[151,1437,196,661]" pageId="7" pageNumber="21">
Measurements.
<emphasis id="32DCE9219438FFDFFE02BA9CFDB9FF23" box="[434,522,196,222]" italics="true" pageId="7" pageNumber="21">Female</emphasis>
(
<typeStatus id="DF138B919438FFDFFDACBA9CFD34FF23" box="[540,647,196,222]" pageId="7" pageNumber="21" type="holotype">holotype</typeStatus>
, CCDB_44300_E09) — Idiosoma L 700; coxal field: L 338; CxIII 400; mL of Cx-I + gnathosoma L 286; distance between lateralmost ends of caudo-lateral Cx-II apodemes, 111; genital field L/W 194/222; genital plates L 113-116; pregenital sclerite W 92; gonopore L 167; L Ac 1-3: 44-46, 50-55, 56.
</paragraph>
<paragraph id="001735339438FFDFFF47BB13FD62FE7A" blockId="7.[151,1437,196,661]" pageId="7" pageNumber="21">Palp — Total L 404; dL/H, dL/H ratio: P-1, 30/35, 0.85; P-2, 106/70, 1.51; P-3, 86/59, 1.45; P-4, 134/33, 4.1; P-5, 48/17, 2.77; P-2/P-4 ratio 0.79.</paragraph>
<paragraph id="001735339438FFDFFF47BBD6FB5DFE55" blockId="7.[151,1437,196,661]" box="[247,1262,398,424]" pageId="7" pageNumber="21">Legs — dL of I-L: 66, 80, 92, 134, 144, 141. dL of IV-L-2-6: 109, 156, 220, 238, 202.</paragraph>
<paragraph id="001735339438FFDFFF45BBE8FB58FDF3" blockId="7.[151,1437,196,661]" pageId="7" pageNumber="21">
Male (
<typeStatus id="DF138B919438FFDFFEF3BBE8FE1EFE37" box="[323,429,432,458]" pageId="7" pageNumber="21" type="paratype">paratype</typeStatus>
, CCDB_39399_B04) — Idiosoma L 594, W 488; coxal field: L 291; Cx-III W 319; mL of Cx-I + gnathosoma L 188; distance between lateralmost ends of caudo-lateral Cx-II apodemes, 101; genital field L/W 111/150, ratio 0.74; L Ac 1-3: 34-38, 48, 38-41. Ejaculatory complex L 134.
</paragraph>
<paragraph id="001735339438FFDFFF47B84DFE5EFD8E" blockId="7.[151,1437,196,661]" pageId="7" pageNumber="21">Palp — Total L 351; dL/H, dL/H ratio: P-1, 25/31, 0.8; P-2, 86/60, 1.43; P-3, 73/53, 1.38; P-4, 123/28, 4.39; P-5, 44/17, 2.55; P-2/P-4 ratio 0.7. Chelicera total L 239, L basal segment 159, claw 80, L basal segment/claw ratio 2.0.</paragraph>
<paragraph id="001735339438FFDFFF47B823FAA9FD68" blockId="7.[151,1437,196,661]" box="[247,1306,635,661]" pageId="7" pageNumber="21">Legs — dL of I-L: 55, 66, 81, 124, 131, 134. dL of IV-L-1-6: 106, 91, 130, 194, 216, 187.</paragraph>
</subSubSection>
<subSubSection id="48B266B89438FFDFFF27B8E6FCF5FACA" pageId="7" pageNumber="21" type="etymology">
<paragraph id="001735339438FFDFFF27B8E6FAE6FD07" blockId="7.[151,1436,702,762]" pageId="7" pageNumber="21">
<emphasis id="32DCE9219438FFDFFF27B8E6FEACFD25" bold="true" box="[151,287,702,728]" pageId="7" pageNumber="21">Etymology</emphasis>
— The new subspecies is named after Prof. dr. Mehmet Zeki Yıldırım (Mehmet Akif Ersoy University,
<collectingRegion id="C26CFBD19438FFDFFE92B8B8FECBFD07" box="[290,376,736,762]" country="Turkey" name="Burdur" pageId="7" pageNumber="21">Burdur</collectingRegion>
,
<collectingCountry id="78BF75A39438FFDFFE33B8B8FE51FD07" box="[387,482,736,762]" name="Turkey" pageId="7" pageNumber="21">Türkiye</collectingCountry>
) in appreciation of his contribution to hydrobiological research of
<collectingCountry id="78BF75A39438FFDFFB40B8B8FAE2FD07" box="[1264,1361,736,762]" name="Turkey" pageId="7" pageNumber="21">Türkiye</collectingCountry>
.
</paragraph>
<paragraph id="001735339438FFDFFF27B97BFE4FFB72" blockId="7.[151,1437,803,1335]" pageId="7" pageNumber="21">
<emphasis id="32DCE9219438FFDFFF27B97BFD16FCC0" bold="true" box="[151,677,803,829]" pageId="7" pageNumber="21">Species delimitation using DNA-barcodes</emphasis>
— The final alignment for the species delimitation using COI sequence data comprised 1774 nucleotide positions (nps) for
<specimenCount id="16AEFEBA9438FFDFFC27B91DFB8FFCA2" box="[919,1084,837,863]" count="36" pageId="7" pageNumber="21" type="generic">36 specimens</specimenCount>
of the
<taxonomicName id="C7A84EB09438FFDFFB11B91DFA20FCA2" authorityName="Piersig" authorityYear="1896" box="[1185,1427,837,863]" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="7" pageNumber="21" phylum="Arthropoda" rank="species" species="calliger">
<emphasis id="32DCE9219438FFDFFB11B91DFA20FCA2" box="[1185,1427,837,863]" italics="true" pageId="7" pageNumber="21">Hygrobates calliger</emphasis>
</taxonomicName>
- complex taken from
<bibRefCitation id="643948C29438FFDFFE25B93FFDC6FC7C" author="Pesic, V. &amp; Esen, Y. &amp; Gerecke, R. &amp; Goldschmidt, T. &amp; Mumladze, L. &amp; Smit, H. &amp; Zawal, A." box="[405,629,871,897]" pageId="7" pageNumber="21" pagination="101 - 122" refId="ref4544" refString="Pesic, V., Esen, Y., Gerecke, R., Goldschmidt, T., Mumladze, L., Smit, H. &amp; Zawal, A. (2022) Evidence of cryptic speciation in the Hygrobates calliger complex (Acariformes, Hydrachnidia, Hygrobatidae) with the description of two new species. Ecologica Montenegrina, 59, 101 - 122. https: // doi. org / 10.37828 / em. 2022.59.10" type="journal article" year="2022">
Pešić
<emphasis id="32DCE9219438FFDFFE6EB93FFDA6FC7C" box="[478,533,871,897]" italics="true" pageId="7" pageNumber="21">et al</emphasis>
. (2022)
</bibRefCitation>
and
<specimenCount id="16AEFEBA9438FFDFFD05B93FFCE4FC7C" box="[693,855,871,897]" count="10" pageId="7" pageNumber="21" type="generic">10 specimens</specimenCount>
from this study (
<tableCitation id="4D2A00889438FFDFFB9DB93FFB3DFC7C" box="[1069,1166,871,897]" captionStart="Table 2" captionStartId="2.[189,254,219,242]" captionText="Table 2. List of newly sequenced specimens in this study." pageId="7" pageNumber="21">Table 2</tableCitation>
).
<specimenCount id="16AEFEBA9438FFDFFB18B93FFAE1FC7C" box="[1192,1362,871,897]" count="1" pageId="7" pageNumber="21" type="generic">One specimen</specimenCount>
of
<taxonomicName id="C7A84EB09438FFDFFA30B93FFE49FC5F" authority="Pesic &amp; Smit, 2017" authorityName="Pesic &amp; Smit" authorityYear="2017" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="7" pageNumber="21" phylum="Arthropoda" rank="species" species="corsicus">
<emphasis id="32DCE9219438FFDFFA30B93FFF4AFC5F" italics="true" pageId="7" pageNumber="21">H. corsicus</emphasis>
<bibRefCitation id="643948C29438FFDFFEB6B9D0FE49FC5F" author="Pesic, V. &amp; Asadi, M. &amp; Cimpean, M. &amp; Dabert, M. &amp; Esen, Y. &amp; Gerecke, R. &amp; Martin, P. &amp; Savic, A. &amp; Smit, H. &amp; Stur, E." box="[262,506,904,930]" pageId="7" pageNumber="21" pagination="1327 - 1377" refId="ref4261" refString="Pesic, V., Asadi, M., Cimpean, M., Dabert, M., Esen, Y., Gerecke, R., Martin, P., Savic, A., Smit, H. &amp; Stur, E. (2017) Six species in one: Evidence of cryptic speciation in the Hygrobates fluviatilis complex (Acariformes, Hydrachnidia, Hygrobatidae). Systematic &amp; Applied Acarology, 22, 1327 - 1377. https: // doi. org / 10.11158 / saa. 22.9.4" type="journal article" year="2017">Pešić &amp; Smit, 2017</bibRefCitation>
</taxonomicName>
(NOVMB011-21) from
<collectingCountry id="78BF75A39438FFDFFC9CB9D0FC35FC5F" box="[812,902,904,930]" name="France" pageId="7" pageNumber="21">Corsica</collectingCountry>
was used as an outgroup. The Neighbour-Joining analyses clustered COI sequences of the analysed specimens into seven maximally supported clades, six of them corresponded to the previously known clades of the
<taxonomicName id="C7A84EB09438FFDFFC2FB994FB93FC1B" authorityName="Piersig" authorityYear="1896" box="[927,1056,972,998]" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="7" pageNumber="21" phylum="Arthropoda" rank="species" species="calliger">
<emphasis id="32DCE9219438FFDFFC2FB994FB93FC1B" box="[927,1056,972,998]" italics="true" pageId="7" pageNumber="21">H. calliger</emphasis>
</taxonomicName>
-complex in
<bibRefCitation id="643948C29438FFDFFB09B994FA2BFC1B" author="Pesic, V. &amp; Esen, Y. &amp; Gerecke, R. &amp; Goldschmidt, T. &amp; Mumladze, L. &amp; Smit, H. &amp; Zawal, A." box="[1209,1432,972,998]" pageId="7" pageNumber="21" pagination="101 - 122" refId="ref4544" refString="Pesic, V., Esen, Y., Gerecke, R., Goldschmidt, T., Mumladze, L., Smit, H. &amp; Zawal, A. (2022) Evidence of cryptic speciation in the Hygrobates calliger complex (Acariformes, Hydrachnidia, Hygrobatidae) with the description of two new species. Ecologica Montenegrina, 59, 101 - 122. https: // doi. org / 10.37828 / em. 2022.59.10" type="journal article" year="2022">
Pešić
<emphasis id="32DCE9219438FFDFFAB1B994FA85FC1B" box="[1281,1334,972,998]" italics="true" pageId="7" pageNumber="21">et al</emphasis>
. (2022)
</bibRefCitation>
. The clade containing sequences of specimens from southwestern
<collectingCountry id="78BF75A39438FFDFFC2FB9B6FC4FFBF5" box="[927,1020,1006,1032]" name="Turkey" pageId="7" pageNumber="21">Türkiye</collectingCountry>
used in this study was placed with high support as a sister to clade grouping sequences of
<taxonomicName id="C7A84EB09438FFDFFC9EBE57FC04FBD4" box="[814,951,1039,1065]" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="7" pageNumber="21" phylum="Arthropoda" rank="species" species="ponticus">
<emphasis id="32DCE9219438FFDFFC9EBE57FC04FBD4" box="[814,951,1039,1065]" italics="true" pageId="7" pageNumber="21">H. ponticus</emphasis>
</taxonomicName>
from
<collectingCountry id="78BF75A39438FFDFFBB2BE57FBD2FBD4" box="[1026,1121,1039,1065]" name="Georgia" pageId="7" pageNumber="21">Georgia</collectingCountry>
and Eastern
<collectingCountry id="78BF75A39438FFDFFB4EBE57FAE8FBD4" box="[1278,1371,1039,1065]" name="Turkey" pageId="7" pageNumber="21">Türkiye</collectingCountry>
(
<figureCitation id="989329B69438FFDFFADDBE57FF1BFBB6" captionStart="Figure 3" captionStartId="6.[151,227,2002,2025]" captionTargetBox="[164,1434,466,1952]" captionTargetId="figure-110@6.[151,1436,459,1984]" captionTargetPageId="6" captionText="Figure 3. Neighbour-joining tree of the Hygrobates calliger-complex obtained from 47 nucleotide COI sequences." figureDoi="http://doi.org/10.5281/zenodo.13247205" httpUri="https://zenodo.org/record/13247205/files/figure.png" pageId="7" pageNumber="21">Fig. 3</figureCitation>
). The COI sequences form a unique cluster (BOLD:AEY7516), with the nearest neighbouring BIN being that of
<taxonomicName id="C7A84EB09438FFDFFF45BE0BFE33FB90" box="[245,384,1107,1133]" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="7" pageNumber="21" phylum="Arthropoda" rank="species" species="ponticus">
<emphasis id="32DCE9219438FFDFFF45BE0BFE33FB90" box="[245,384,1107,1133]" italics="true" pageId="7" pageNumber="21">H. ponticus</emphasis>
</taxonomicName>
(BOLD:AEO0649). The genetic distance between these two clades was estimated at 10.2±1.4% K2P (see
<tableCitation id="4D2A00889438FFDFFE21BE2DFE5FFB72" box="[401,492,1141,1167]" captionStart="Table 3" captionStartId="2.[189,254,886,909]" captionText="Table 3. Estimates of average genetic distance (K2P) (given as distance ± standard error) within (intragroup) and between calliger-complex sequence pairs. Standard error estimates (for intergroup) are shown above the diagonal. There were a" pageId="7" pageNumber="21">Table 3</tableCitation>
).
</paragraph>
<paragraph id="001735339438FFDFFF47BECEFCF5FACA" blockId="7.[151,1437,803,1335]" pageId="7" pageNumber="21">
The ASAP delimitation method identified MOTU (molecular operational taxonomic units) which matched the clades in
<bibRefCitation id="643948C29438FFDFFE11BEE0FDCDFB2F" author="Pesic, V. &amp; Esen, Y. &amp; Gerecke, R. &amp; Goldschmidt, T. &amp; Mumladze, L. &amp; Smit, H. &amp; Zawal, A." box="[417,638,1208,1234]" pageId="7" pageNumber="21" pagination="101 - 122" refId="ref4544" refString="Pesic, V., Esen, Y., Gerecke, R., Goldschmidt, T., Mumladze, L., Smit, H. &amp; Zawal, A. (2022) Evidence of cryptic speciation in the Hygrobates calliger complex (Acariformes, Hydrachnidia, Hygrobatidae) with the description of two new species. Ecologica Montenegrina, 59, 101 - 122. https: // doi. org / 10.37828 / em. 2022.59.10" type="journal article" year="2022">
Pešić
<emphasis id="32DCE9219438FFDFFE57BEE0FDAFFB2F" box="[487,540,1208,1234]" italics="true" pageId="7" pageNumber="21">et al</emphasis>
. (2022)
</bibRefCitation>
. The best ASAP score had 3.50 at a threshold distance of 12.106% K2P identified
<taxonomicName id="C7A84EB09438FFDFFEFBBE82FE61FB09" box="[331,466,1242,1268]" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="7" pageNumber="21" phylum="Arthropoda" rank="species" species="ponticus">
<emphasis id="32DCE9219438FFDFFEFBBE82FE61FB09" box="[331,466,1242,1268]" italics="true" pageId="7" pageNumber="21">H. ponticus</emphasis>
</taxonomicName>
clade from
<collectingCountry id="78BF75A39438FFDFFDEFBE82FD0DFB09" box="[607,702,1242,1268]" name="Georgia" pageId="7" pageNumber="21">Georgia</collectingCountry>
and Eastern
<collectingCountry id="78BF75A39438FFDFFCE6BE82FC00FB09" box="[854,947,1242,1268]" name="Turkey" pageId="7" pageNumber="21">Türkiye</collectingCountry>
and the clade from southwestern
<collectingCountry id="78BF75A39438FFDFFAF0BE82FA2EFB09" box="[1344,1437,1242,1268]" name="Turkey" pageId="7" pageNumber="21">Türkiye</collectingCountry>
as a single MOTU. On the other hand, the second best score of ASAP (6.0) a threshold distance of 6.885% K2P separated the latter two clades into different MOTUs.
</paragraph>
</subSubSection>
<caption id="54D765BB9438FFDFFF27BDE6FEEFF809" ID-DOI="http://doi.org/10.5281/zenodo.13247207" ID-Zenodo-Dep="13247207" httpUri="https://zenodo.org/record/13247207/files/figure.png" pageId="7" pageNumber="21" startId="7.[151,227,1982,2005]" targetBox="[151,1436,1370,1964]" targetPageId="7" targetType="figure">
<paragraph id="001735339438FFDFFF27BDE6FEEFF809" blockId="7.[151,1437,1982,2036]" pageId="7" pageNumber="21">
<emphasis id="32DCE9219438FFDFFF27BDE6FF44F828" bold="true" box="[151,247,1982,2005]" pageId="7" pageNumber="21">Figure 4</emphasis>
. Photograph of the
<emphasis id="32DCE9219438FFDFFE7BBDE6FDE5F828" box="[459,598,1982,2005]" italics="true" pageId="7" pageNumber="21">locus typicus</emphasis>
of
<taxonomicName id="C7A84EB09438FFDFFDCABDE6FC08F828" authorityName="Pešić &amp; Zawal &amp; Gülle &amp; Cakmak &amp; Saboori" authorityYear="2023" box="[634,955,1982,2005]" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="7" pageNumber="21" phylum="Arthropoda" rank="subSpecies" species="ponticus" status="subsp. nov." subSpecies="yildirimi">
<emphasis id="32DCE9219438FFDFFDCABDE6FC08F828" box="[634,955,1982,2005]" italics="true" pageId="7" pageNumber="21">Hygrobates ponticus yildirimi</emphasis>
</taxonomicName>
<taxonomicNameLabel id="29EF545A9438FFDFFC71BDE6FBF2F828" box="[961,1089,1982,2005]" pageId="7" pageNumber="21" rank="subSpecies">
<emphasis id="32DCE9219438FFDFFC71BDE6FB8AF828" bold="true" box="[961,1081,1982,2005]" pageId="7" pageNumber="21">subsp. nov</emphasis>
.
</taxonomicNameLabel>
: Aksu Stream, Isparta Province. Photo by P. Gülle.
</paragraph>
</caption>
<subSubSection id="48B266B89437FFD0FF27BA9CFAB7FDAC" pageId="8" pageNumber="22" type="discussion">
<paragraph id="001735339437FFD0FF27BA9CFD71FE55" blockId="8.[151,1437,196,593]" pageId="8" pageNumber="22">
<emphasis id="32DCE9219437FFD0FF27BA9CFEA8FF23" bold="true" box="[151,283,196,222]" pageId="8" pageNumber="22">Discussion</emphasis>
— The molecular analysis based on COI data placed the specimens of
<taxonomicName id="C7A84EB09437FFD0FBDDBA9CFB5DFF23" authorityName="Piersig" authorityYear="1896" box="[1133,1262,196,222]" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="8" pageNumber="22" phylum="Arthropoda" rank="species" species="calliger">
<emphasis id="32DCE9219437FFD0FBDDBA9CFB5DFF23" box="[1133,1262,196,222]" italics="true" pageId="8" pageNumber="22">H. calliger</emphasis>
</taxonomicName>
complex from southwestern
<collectingCountry id="78BF75A39437FFD0FE89BABEFE25FEFD" box="[313,406,230,256]" name="Turkey" pageId="8" pageNumber="22">Türkiye</collectingCountry>
used in this study for molecular analysis as sister to a clade containing specimens of
<taxonomicName id="C7A84EB09437FFD0FA30BABEFDC9FEDF" authority="Pesic, Esen &amp; Mumladze, 2022" authorityName="Pesic, Esen &amp; Mumladze" authorityYear="2022" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="8" pageNumber="22" phylum="Arthropoda" rank="species" species="ponticus">
<emphasis id="32DCE9219437FFD0FA30BABEFF48FEDF" italics="true" pageId="8" pageNumber="22">H. ponticus</emphasis>
<bibRefCitation id="643948C29437FFD0FEB3BB50FDC9FEDF" author="Pesic, V. &amp; Esen, Y. &amp; Gerecke, R. &amp; Goldschmidt, T. &amp; Mumladze, L. &amp; Smit, H. &amp; Zawal, A." box="[259,634,264,290]" pageId="8" pageNumber="22" pagination="101 - 122" refId="ref4544" refString="Pesic, V., Esen, Y., Gerecke, R., Goldschmidt, T., Mumladze, L., Smit, H. &amp; Zawal, A. (2022) Evidence of cryptic speciation in the Hygrobates calliger complex (Acariformes, Hydrachnidia, Hygrobatidae) with the description of two new species. Ecologica Montenegrina, 59, 101 - 122. https: // doi. org / 10.37828 / em. 2022.59.10" type="journal article" year="2022">Pešić, Esen &amp; Mumladze, 2022</bibRefCitation>
</taxonomicName>
from
<collectingCountry id="78BF75A39437FFD0FD74BB50FC90FEDF" box="[708,803,264,290]" name="Georgia" pageId="8" pageNumber="22">Georgia</collectingCountry>
and Eastern
<collectingCountry id="78BF75A39437FFD0FC0FBB50FB93FEDF" box="[959,1056,264,290]" name="Turkey" pageId="8" pageNumber="22">Türkiye</collectingCountry>
. Morphologically, specimens of the latter clade resemble specimens from southwestern
<collectingCountry id="78BF75A39437FFD0FCF8BB71FC16FEBE" box="[840,933,297,323]" name="Turkey" pageId="8" pageNumber="22">Türkiye</collectingCountry>
by relatively long gonopore in females, which exceed the length of the genital plate. However, in the new subspecies, the ventrodistal projection of P-2 tends to be somewhat longer in comparison to the
<taxonomicName id="C7A84EB09437FFD0FC98BB35FC02FE7A" authorityName="Pesic, Esen &amp; Mumladze" authorityYear="2022" box="[808,945,365,391]" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="8" pageNumber="22" phylum="Arthropoda" rank="species" species="ponticus">
<emphasis id="32DCE9219437FFD0FC98BB35FC02FE7A" box="[808,945,365,391]" italics="true" pageId="8" pageNumber="22">H. ponticus</emphasis>
</taxonomicName>
clade from
<collectingCountry id="78BF75A39437FFD0FBF3BB35FB11FE7A" box="[1091,1186,365,391]" name="Georgia" pageId="8" pageNumber="22">Georgia</collectingCountry>
and Eastern
<collectingCountry id="78BF75A39437FFD0FA8FBB35FA2FFE7A" box="[1343,1436,365,391]" name="Turkey" pageId="8" pageNumber="22">Türkiye</collectingCountry>
(L/W ratio: 1.2
<emphasis id="32DCE9219437FFD0FEFFBBD6FEDBFE55" box="[335,360,398,424]" italics="true" pageId="8" pageNumber="22">vs</emphasis>
. 1.0 in
<emphasis id="32DCE9219437FFD0FE71BBD6FDD6FE55" box="[449,613,398,424]" italics="true" pageId="8" pageNumber="22">
H. p.
<taxonomicName id="C7A84EB09437FFD0FDB1BBD6FDD6FE55" authorityName="Pesic, Esen &amp; Mumladze" authorityYear="2022" box="[513,613,398,424]" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="8" pageNumber="22" phylum="Arthropoda" rank="species" species="ponticus">ponticus</taxonomicName>
</emphasis>
Clade).
</paragraph>
<paragraph id="001735339437FFD0FF47BBE8FAB7FDAC" blockId="8.[151,1437,196,593]" pageId="8" pageNumber="22">
Based on the molecular and morphological evidence and the geographical isolation of the clades (allopatric populations), we propose the presence of two subspecies:
<taxonomicName id="C7A84EB09437FFD0FC59BB8AFED3FDF3" authority="Pesic, Esen &amp; Mumladze, 2022" authorityName="Pesic, Esen &amp; Mumladze" authorityYear="2022" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="8" pageNumber="22" phylum="Arthropoda" rank="subSpecies" species="ponticus" subSpecies="ponticus">
<emphasis id="32DCE9219437FFD0FC59BB8AFB50FE11" box="[1001,1251,466,492]" italics="true" pageId="8" pageNumber="22">H. ponticus ponticus</emphasis>
<bibRefCitation id="643948C29437FFD0FB40BB8AFED3FDF3" author="Pesic, V. &amp; Esen, Y. &amp; Gerecke, R. &amp; Goldschmidt, T. &amp; Mumladze, L. &amp; Smit, H. &amp; Zawal, A." pageId="8" pageNumber="22" pagination="101 - 122" refId="ref4544" refString="Pesic, V., Esen, Y., Gerecke, R., Goldschmidt, T., Mumladze, L., Smit, H. &amp; Zawal, A. (2022) Evidence of cryptic speciation in the Hygrobates calliger complex (Acariformes, Hydrachnidia, Hygrobatidae) with the description of two new species. Ecologica Montenegrina, 59, 101 - 122. https: // doi. org / 10.37828 / em. 2022.59.10" type="journal article" year="2022">Pešić, Esen &amp; Mumladze, 2022</bibRefCitation>
</taxonomicName>
(
<collectingCountry id="78BF75A39437FFD0FEC2BBACFE66FDF3" box="[370,469,500,526]" name="Georgia" pageId="8" pageNumber="22">Georgia</collectingCountry>
, Eastern
<collectingCountry id="78BF75A39437FFD0FDF4BBACFD10FDF3" box="[580,675,500,526]" name="Turkey" pageId="8" pageNumber="22">Türkiye</collectingCountry>
) and
<taxonomicName id="C7A84EB09437FFD0FD59BBACFC52FDF3" authorityName="Pešić &amp; Zawal &amp; Gülle &amp; Cakmak &amp; Saboori" authorityYear="2023" box="[745,993,500,526]" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" pageId="8" pageNumber="22" phylum="Arthropoda" rank="subSpecies" species="ponticus" subSpecies="yildirimi">
<emphasis id="32DCE9219437FFD0FD59BBACFC52FDF3" box="[745,993,500,526]" italics="true" pageId="8" pageNumber="22">H. ponticus yildirimi</emphasis>
</taxonomicName>
<emphasis id="32DCE9219437FFD0FC5BBBACFBE2FDF3" bold="true" box="[1003,1105,500,526]" pageId="8" pageNumber="22">ssp. nov</emphasis>
. (southwestern
<collectingCountry id="78BF75A39437FFD0FABFBBACFAC1FDF3" box="[1295,1394,500,526]" name="Turkey" pageId="8" pageNumber="22">Türkiye</collectingCountry>
). A possible future discovery of the co-occurrence of these clades/morphotypes could indicate their reproductive isolation and would potentially justify their subsequent elevation to the status of distinct species.
</paragraph>
</subSubSection>
<subSubSection id="48B266B89437FFD0FF27B823FC63FD68" box="[151,976,635,661]" pageId="8" pageNumber="22" type="distribution">
<paragraph id="001735339437FFD0FF27B823FC63FD68" blockId="8.[151,976,635,661]" box="[151,976,635,661]" pageId="8" pageNumber="22">
<emphasis id="32DCE9219437FFD0FF27B823FE82FD68" bold="true" box="[151,305,635,661]" pageId="8" pageNumber="22">Distribution</emphasis>
— Southwestern
<collectingCountry id="78BF75A39437FFD0FDB3B823FDD3FD68" box="[515,608,635,661]" name="Turkey" pageId="8" pageNumber="22">Türkiye</collectingCountry>
(
<collectingRegion id="C26CFBD19437FFD0FDDFB823FD09FD68" box="[623,698,635,661]" country="Turkey" name="Aydin" pageId="8" pageNumber="22">Aydin</collectingRegion>
and
<collectingRegion id="C26CFBD19437FFD0FD44B823FCF7FD68" box="[756,836,635,661]" country="Turkey" name="Isparta" pageId="8" pageNumber="22">Isparta</collectingRegion>
Provinces).
</paragraph>
</subSubSection>
</treatment>
</document>