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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.43.25081" ID-GBIF-Dataset="d9c55c7b-4a9f-4045-bd20-09dd6902b3fe" ID-PMC="PMC6290043" ID-Pensoft-Pub="1314-4049--23" ID-PubMed="30568535" ModsDocAuthor="" ModsDocDate="2018" ModsDocID="1314-4049--23" ModsDocOrigin="MycoKeys " ModsDocTitle="Endophytic Colletotrichum species from Dendrobium spp. in China and Northern Thailand" checkinTime="1553125590011" checkinUser="pensoft" docAuthor="Ma, Xiaoya, Nontachaiyapoom, Sureeporn, Jayawardena, Ruvishika S., yde, Kevin D., Gentekaki, Eleni, Zhou, Sixuan, Qian, Yixin, Wen, Tingchi &amp; Kang, Jichuan" docDate="2018" docId="6EEC292788F7263A973096FD2FE2B900" docLanguage="en" docName="MycoKeys 43: 23-57" docOrigin="MycoKeys 43" docSource="http://dx.doi.org/10.3897/mycokeys.43.25081" docTitle="Colletotrichum chiangraiense X. Y. Ma, K. D. Hyde &amp; Jayawardena, sp.nov." docType="treatment" docVersion="4" lastPageNumber="30" masterDocId="FF97FFBBFFBCFFCF2B24FFA89B70FFD8" masterDocTitle="Endophytic Colletotrichum species from Dendrobium spp. in China and Northern Thailand" masterLastPageNumber="57" masterPageNumber="23" pageNumber="30" updateTime="1668136111028" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Endophytic Colletotrichum species from Dendrobium spp. in China and Northern Thailand</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Ma, Xiaoya</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Nontachaiyapoom, Sureeporn</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Jayawardena, Ruvishika S.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>yde, Kevin D.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Gentekaki, Eleni</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Zhou, Sixuan</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Qian, Yixin</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Wen, Tingchi</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Kang, Jichuan</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2018</mods:date>
<mods:detail type="volume">
<mods:number>43</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>23</mods:start>
<mods:end>57</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.43.25081</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.43.25081</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049--23</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="154473872" LSID="urn:lsid:plazi:treatment:6EEC292788F7263A973096FD2FE2B900" httpUri="http://treatment.plazi.org/id/6EEC292788F7263A973096FD2FE2B900" lastPageNumber="30" pageId="7" pageNumber="30">
<subSubSection pageId="7" pageNumber="30" type="nomenclature">
<paragraph pageId="7" pageNumber="30">
<taxonomicName authority="X. Y. Ma, K. D. Hyde &amp; Jayawardena" class="Sordariomycetes" family="Glomerellaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum chiangraiense" order="Glomerellales" pageId="7" pageNumber="30" phylum="Ascomycota" rank="species" species="chiangraiense">Colletotrichum chiangraiense X.Y. Ma, K.D. Hyde &amp; Jayawardena</taxonomicName>
<taxonomicNameLabel pageId="7" pageNumber="30">sp.nov.</taxonomicNameLabel>
Figure 3
</paragraph>
</subSubSection>
<subSubSection pageId="7" pageNumber="30" type="etymology">
<paragraph pageId="7" pageNumber="30">Etymology.</paragraph>
<paragraph pageId="7" pageNumber="30">In reference to the host sample site Chiang Rai, Thailand.</paragraph>
</subSubSection>
<subSubSection pageId="7" pageNumber="30" type="holotype">
<paragraph pageId="7" pageNumber="30">Holotype.</paragraph>
<paragraph pageId="7" pageNumber="30">MFLU 17-1201 (ex-holotype culture: MFLUCC 14-0119).</paragraph>
</subSubSection>
<subSubSection pageId="7" pageNumber="30" type="description">
<paragraph pageId="7" pageNumber="30">Description.</paragraph>
<paragraph pageId="7" pageNumber="30">Asexual morph not observed.</paragraph>
<paragraph pageId="7" pageNumber="30">
Sexual morph on CMA. Vegetative hyphae 4.6.
<normalizedToken originalValue="±">+/-</normalizedToken>
1.8
<normalizedToken originalValue="µm">µm</normalizedToken>
diam (N=20), hyaline to pale brown, smooth-walled, septate, branched. Chlamydospore globose, brown. Hyphae fusion and crozier observed. Ascomata rare, covered by mycelia, black. Appressoria (14.4-) 15.3-19.6 (-20.5)
<normalizedToken originalValue="×">x</normalizedToken>
(7.4-) 7.3-7.9 (-8)
<normalizedToken originalValue="µm">µm</normalizedToken>
(N=2), single, outline ampulliform or ovate, pale brown. Asci (30.7-) 33.4-52.7 (-72.18)
<normalizedToken originalValue="×">x</normalizedToken>
(5.7-) 6.5-8.2 (-9.4)
<normalizedToken originalValue="µm">µm</normalizedToken>
(N=15), cylindrical, straight to curved, unitunicate, 8-spored. Ascospores (11-) 11.9-15.4 (-16.7)
<normalizedToken originalValue="×">x</normalizedToken>
(2.2-) 2.8-3.8 (-4.4)
<normalizedToken originalValue="µm">µm</normalizedToken>
(N=20), L/W = 4.2, bi-seriately, smooth-walled, cylindrical or fusiform, one guttule in the middle, hyaline.
</paragraph>
<paragraph pageId="7" pageNumber="30">Cultures on CMA flat with entire margin. Growth rate: 0.6cm/day, with 20-days for sporulation. Fluffy, dark green in the middle and white margin, reverse black in the middle.</paragraph>
<caption pageId="7" pageNumber="30">
<paragraph pageId="7" pageNumber="30">
Figure 3.
<taxonomicName class="Dothideomycetes" family="Botryosphaeriaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum chiangraiense" order="Botryosphaeriales" pageId="7" pageNumber="30" phylum="Ascomycota" rank="species" species="chiangraiense">Colletotrichum chiangraiense</taxonomicName>
(holotype). A Colony B Spore germination C Conidiophore D Appressoria E Chlamydospore F Mycelia fusion G Crozier
<normalizedToken originalValue="HN">H-N</normalizedToken>
Asci
<normalizedToken originalValue="OR">O-R</normalizedToken>
Ascospores. Scale bars: 20
<normalizedToken originalValue="µm">µm</normalizedToken>
(D), 20
<normalizedToken originalValue="µm">µm</normalizedToken>
(G), 5
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<normalizedToken originalValue="JN">J-N</normalizedToken>
), 10
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<normalizedToken originalValue="OR">O-R</normalizedToken>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection pageId="7" pageNumber="30" type="materials_examined">
<paragraph pageId="7" pageNumber="30">Material examined.</paragraph>
<paragraph pageId="7" pageNumber="30">
Thailand, Chiang Rai, Wat Phra That Doi Tung (Temple of Doi Tung Pagodas), the host
<taxonomicName class="Liliopsida" family="Orchidaceae" genus="Dendrobium" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Dendrobium" order="Asparagales" pageId="7" pageNumber="30" phylum="Tracheophyta" rank="genus">Dendrobium</taxonomicName>
sp. 2 was collected on 19 December 2013, Collector: Sureeporn Nontachaiyapoom, Natdanai Aewsakul, Xiaoya Ma.
</paragraph>
</subSubSection>
<subSubSection pageId="7" pageNumber="30" type="notes">
<paragraph pageId="7" pageNumber="30">Notes.</paragraph>
<paragraph pageId="7" pageNumber="30">
<taxonomicName class="Dothideomycetes" family="Botryosphaeriaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum chiangraiense" order="Botryosphaeriales" pageId="7" pageNumber="30" phylum="Ascomycota" rank="species" species="chiangraiense">Colletotrichum chiangraiense</taxonomicName>
(MFLUCC 14-0119) formed a single branch with the support of 81ML/1.00BI/78MP in
<taxonomicName class="Sordariomycetes" family="Glomerellaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum boninense" order="Glomerellales" pageId="7" pageNumber="30" phylum="Ascomycota" rank="species" species="boninense">boninense</taxonomicName>
species complex. It has 125 different base pairs (mainly in ITS and ACT) from the closest strain
<taxonomicName lsidName="C. cymbidiicola" pageId="7" pageNumber="30" rank="species" species="cymbidiicola">C. cymbidiicola</taxonomicName>
. Blastn searches with sequences of MFLUCC 14-0119 resulted in 99% identity with ITS of the endophytic
<taxonomicName lsidName="C. crassipes" pageId="7" pageNumber="30" rank="species" species="crassipes">C. crassipes</taxonomicName>
strain DO93 (GenBank KP050648) isolated from
<taxonomicName class="Liliopsida" family="Orchidaceae" genus="Dendrobium" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Dendrobium officinale" order="Asparagales" pageId="7" pageNumber="30" phylum="Tracheophyta" rank="species" species="officinale">Dendrobium officinale</taxonomicName>
in China (Unpublished), 99% identity with ACT of the endophytic
<taxonomicName class="Dothideomycetes" family="Botryosphaeriaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum" order="Botryosphaeriales" pageId="7" pageNumber="30" phylum="Ascomycota" rank="genus">Colletotrichum</taxonomicName>
sp. strain COAD 2105 (GenBank KY407893) isolated from
<taxonomicName class="Liliopsida" family="Orchidaceae" genus="Cattleya" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Cattleya jongheana" order="Asparagales" pageId="7" pageNumber="30" phylum="Tracheophyta" rank="species" species="jongheana">Cattleya jongheana</taxonomicName>
in Brazil (Unpublished), 98% identity with TUB2 of the endophytic
<taxonomicName lsidName="C. boninense" pageId="7" pageNumber="30" rank="species" species="boninense">C. boninense</taxonomicName>
strain CBS 125502 (GenBank KJ955336) isolated from
<taxonomicName class="Magnoliopsida" family="Theaceae" genus="Camellia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Camellia sinensis" order="Ericales" pageId="7" pageNumber="30" phylum="Tracheophyta" rank="species" species="sinensis">Camellia sinensis</taxonomicName>
in China (
<bibRefCitation author="Liu, F" journalOrPublisher="Persoonia-Molecular Phylogeny and Evolution of Fungi" pageId="24" pageNumber="47" pagination="63 - 86" title="Unravelling Colletotrichum species associated with Camellia: employing ApMat and GS loci to resolve species in the C. gloeosporioides complex." url="https://doi.org/10.3767/003158515X687597" volume="35" year="2015">Liu et al. 2015</bibRefCitation>
) and 98% identity with TUB2 of the endophytic
<taxonomicName lsidName="C. boninense" pageId="7" pageNumber="30" rank="species" species="boninense">C. boninense</taxonomicName>
strain CGMCC 3.15165 (GenBank KC244156) isolated from
<taxonomicName class="Liliopsida" family="Orchidaceae" genus="Bletilla" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Bletilla ochracea" order="Asparagales" pageId="7" pageNumber="30" phylum="Tracheophyta" rank="species" species="ochracea">Bletilla ochracea</taxonomicName>
in China (
<bibRefCitation author="Tao, G" journalOrPublisher="Fungal Diversity" pageId="26" pageNumber="49" pagination="139 - 164" title="Endophytic Colletotrichum species from Bletillaochracea (Orchidaceae) with descriptions of seven new species." url="https://doi.org/10.1007/s13225-013-0254-5" volume="61" year="2013">Tao et al. 2013</bibRefCitation>
). This species was observed antheridium, mycelia fusion and crozier, which indicates that this species may be homothallic. Here we introduce the strain isolated from root of
<taxonomicName class="Liliopsida" family="Orchidaceae" genus="Dendrobium" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Dendrobium" order="Asparagales" pageId="7" pageNumber="30" phylum="Tracheophyta" rank="genus">Dendrobium</taxonomicName>
sp. 2 as a new species.
</paragraph>
</subSubSection>
</treatment>
</document>