124 lines
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124 lines
12 KiB
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<document ID-DOI="http://dx.doi.org/10.3897/zookeys.1015.54387" ID-GBIF-Dataset="7f0a1826-ba89-4ecb-adbb-275211293c87" ID-PMC="PMC7878468" ID-Pensoft-Pub="1313-2970-1015-1" ID-Pensoft-UUID="AF1641758561525C8D40BBF3F895FA8A" ID-PubMed="33613041" ID-ZooBank="F6BD92139DB74564AA003C61B2F43B2D" ModsDocID="1313-2970-1015-1" checkinTime="1612497320802" checkinUser="pensoft" docAuthor="Abe, Hirokazu & Sato-Okoshi, Waka" docDate="2021" docId="32472EAF760E51579E91C5DDCB61A440" docLanguage="en" docName="ZooKeys 1015: 1-86" docOrigin="ZooKeys 1015" docPubDate="2021-02-04" docSource="http://dx.doi.org/10.3897/zookeys.1015.54387" docTitle="Prionospio spp. 1 and 2" docType="treatment" docVersion="5" id="AF1641758561525C8D40BBF3F895FA8A" lastPageNumber="1" masterDocId="AF1641758561525C8D40BBF3F895FA8A" masterDocTitle="Molecular identification and larval morphology of spionid polychaetes (Annelida, Spionidae) from northeastern Japan" masterLastPageNumber="86" masterPageNumber="1" pageNumber="1" updateTime="1668149773418" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Molecular identification and larval morphology of spionid polychaetes (Annelida, Spionidae) from northeastern Japan</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Abe, Hirokazu</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-7753-9368</mods:nameIdentifier>
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<mods:affiliation>Department of Biology, Center for Liberal Arts & Sciences, Iwate Medical University, Idaidori 1 - 1 - 1, Yahaba-cho, Shiwa-gun, Iwate 028 - 3694, Japan</mods:affiliation>
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<mods:nameIdentifier type="email">habe@iwate-med.ac.jp</mods:nameIdentifier>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Sato-Okoshi, Waka</mods:namePart>
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<mods:affiliation>Laboratory of Biological Oceanography, Graduate School of Agricultural Science, Tohoku University, Aramaki-Aza-Aoba 468 - 1, Aoba-ku, Sendai 980 - 8572, Japan</mods:affiliation>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>ZooKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2021</mods:date>
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<mods:detail type="pubDate">
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<mods:number>2021-02-04</mods:number>
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</mods:detail>
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<mods:detail type="volume">
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<mods:number>1015</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>1</mods:start>
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<mods:end>86</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/zookeys.1015.54387</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/zookeys.1015.54387</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1313-2970-1015-1</mods:identifier>
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<mods:identifier type="ZooBank">F6BD92139DB74564AA003C61B2F43B2D</mods:identifier>
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<mods:identifier type="Pensoft-UUID">AF1641758561525C8D40BBF3F895FA8A</mods:identifier>
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<treatment ID-GBIF-Taxon="177212506" LSID="urn:lsid:plazi:treatment:32472EAF760E51579E91C5DDCB61A440" httpUri="http://treatment.plazi.org/id/32472EAF760E51579E91C5DDCB61A440" lastPageNumber="1" pageId="0" pageNumber="1">
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<subSubSection pageId="0" pageNumber="1" type="nomenclature">
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<paragraph pageId="0" pageNumber="1">
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<taxonomicName LSID="32472EAF-760E-5157-9E91-C5DDCB61A440" authority="spp. 1 and 2" authorityName="spp. 1 and 2" class="Polychaeta" family="Spionidae" genus="Prionospio" higherTaxonomySource="treatment-meta" kingdom="Animalia" lsidName="Prionospio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">Prionospio spp. 1 and 2</taxonomicName>
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<figureCitation captionStart="Figure 5" captionStartId="F6" captionText="Figure 5. Light micrographs showing the morphologies of living spionid larvae of genera Paraprionospio and Prionospio A, B Paraprionospio coora, lateral view of 25 - chaetiger (A) and 33 - chaetiger larvae (B) C, D Prionospio krusadensis, lateral view of 17 - chaetiger larvae E-G Prionospio membranacea, lateral view of 15 - chaetiger (E), 20 - chaetiger (F), and 24 - chaetiger larvae (G) H, Prionospio sp. 1, lateral view of 11 - chaetiger larva I Prionospio sp. 2, lateral view of 19 - chaetiger larva. Scale bars: 300 μm." figureDoi="10.3897/zookeys.1015.54387.figure5" httpUri="https://binary.pensoft.net/fig/505192" pageId="0" pageNumber="1">Fig. 5H, I</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="1" type="larval morphology">
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<paragraph pageId="0" pageNumber="1">Larval morphology.</paragraph>
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<paragraph pageId="0" pageNumber="1">
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Long and thin in shape. Prostomium rounded anteriorly, lateral parts of the peristomium not especially well demarcated. Two pairs of red or dark red eyes arranged somewhat in trapezoidal shape, lateral pair kidney-shaped and situated anteriorly. Small caruncle develop in late larvae, extends posteriorly from posterior part of the prostomium. In
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<taxonomicName class="Polychaeta" family="Spionidae" genus="Prionospio" kingdom="Animalia" lsidName="Prionospio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="1">Prionospio</emphasis>
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</taxonomicName>
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sp. 2, branchial anlages occur from chaetiger II, pygidium acquires anal cirri (Fig.
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<figureCitation captionStart="Figure 5" captionStartId="F6" captionText="Figure 5. Light micrographs showing the morphologies of living spionid larvae of genera Paraprionospio and Prionospio A, B Paraprionospio coora, lateral view of 25 - chaetiger (A) and 33 - chaetiger larvae (B) C, D Prionospio krusadensis, lateral view of 17 - chaetiger larvae E-G Prionospio membranacea, lateral view of 15 - chaetiger (E), 20 - chaetiger (F), and 24 - chaetiger larvae (G) H, Prionospio sp. 1, lateral view of 11 - chaetiger larva I Prionospio sp. 2, lateral view of 19 - chaetiger larva. Scale bars: 300 μm." figureDoi="10.3897/zookeys.1015.54387.figure5" httpUri="https://binary.pensoft.net/fig/505192" pageId="0" pageNumber="1">5I</figureCitation>
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). Pigmentation absent except eyes in
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<taxonomicName class="Polychaeta" family="Spionidae" genus="Prionospio" kingdom="Animalia" lsidName="Prionospio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="1">Prionospio</emphasis>
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</taxonomicName>
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sp. 1. Gut pigmented in orange in
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<taxonomicName class="Polychaeta" family="Spionidae" genus="Prionospio" kingdom="Animalia" lsidName="Prionospio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="1">Prionospio</emphasis>
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</taxonomicName>
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sp. 2. Palps developed in 19-chaetiger larva of
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<taxonomicName class="Polychaeta" family="Spionidae" genus="Prionospio" kingdom="Animalia" lsidName="Prionospio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="1">Prionospio</emphasis>
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</taxonomicName>
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sp. 2.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="1" type="remarks">
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<paragraph pageId="0" pageNumber="1">Remarks.</paragraph>
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<paragraph pageId="0" pageNumber="1">
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Two unidentified species of planktonic larvae of the genus
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<taxonomicName class="Polychaeta" family="Spionidae" genus="Prionospio" kingdom="Animalia" lsidName="Prionospio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="1">Prionospio</emphasis>
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</taxonomicName>
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other than
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<taxonomicName class="Polychaeta" higherTaxonomySource="GBIF,CoL" kingdom="Animalia" lsidName="P. krusadensis" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="krusadensis">
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<emphasis italics="true" pageId="0" pageNumber="1">P. krusadensis</emphasis>
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</taxonomicName>
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and
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<taxonomicName class="Polychaeta" higherTaxonomySource="GBIF,CoL" kingdom="Animalia" lsidName="P. membranacea" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="membranacea">
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<emphasis italics="true" pageId="0" pageNumber="1">P. membranacea</emphasis>
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</taxonomicName>
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were collected from Onagawa Bay. The adult individuals of these species were not collected in the present study. Even though the 18S and 16S rRNA gene sequences obtained from these larvae in the present study did not match any
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<taxonomicName class="Polychaeta" family="Spionidae" genus="Prionospio" kingdom="Animalia" lsidName="Prionospio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="1">Prionospio</emphasis>
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</taxonomicName>
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sequences obtained in the present study nor with those registered in DDBJ/EMBL/GenBank, these species were referred to
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<taxonomicName class="Polychaeta" family="Spionidae" genus="Prionospio" kingdom="Animalia" lsidName="Prionospio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="1">Prionospio</emphasis>
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</taxonomicName>
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sp. 1 and 2 as the larvae were similar to the other
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<taxonomicName class="Polychaeta" family="Spionidae" genus="Prionospio" kingdom="Animalia" lsidName="Prionospio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="1">Prionospio</emphasis>
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</taxonomicName>
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species in their morphology and gene sequences (Figs
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<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Maximum Likelihood tree inferred from nuclear 18 S and mitochondrial 16 S rRNA gene sequences of spionids obtained from Japan in the present and previous studies (provided in Table 1). The gene sequences of adult and larval spionid polychaetes are indicated by solid squares and circles in front of each species name, respectively. SH-aLRT / approximate Bayes support / ultrafast bootstrap support values of ≥ 80 %, ≥ 0.95, ≥ 95 %, respectively are given beside the respective nodes. Nodes with red circles indicate triple high support values of SH-aLRT ≥ 80, approximate Bayes support ≥ 0.95, and ultrafast bootstrap support ≥ 95. The scale bar represents the number of substitutions per site. Sequences of Laonome sp. and Sabella pavonina Savigny, 1822 obtained from DDBJ / EMBL / GenBank were used for outgroup rooting." figureDoi="10.3897/zookeys.1015.54387.figure2" httpUri="https://binary.pensoft.net/fig/505188" pageId="0" pageNumber="1">2</figureCitation>
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,
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<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Maximum Likelihood tree inferred from nuclear 18 S and mitochondrial 16 S rRNA gene sequences of spionid polychaetes obtained from Japan in the present and previous studies (shown in Table 1) and from DDBJ / EMBL / GenBank (shown in Table 2). The tree is divided into two parts A, B. The gene sequences obtained in the present study are highlighted by bold and red color and the adult and larval sequences are indicated by solid squares and circles in front of each species name, respectively. SH-aLRT / approximate Bayes support / ultrafast bootstrap support values of ≥ 80 % / ≥ 0.95 / ≥ 95 %, respectively are given beside the respective nodes. Nodes with red circles indicate triple high support values of SH-aLRT ≥ 80, approximate Bayes support ≥ 0.95, and ultrafast bootstrap support ≥ 95. The scale bar represents the number of substitutions per site. Sequences of Laonome sp. and Sabella pavonina Savigny, 1822 obtained from GenBank were used for outgroup rooting." figureDoi="10.3897/zookeys.1015.54387.figure3" httpUri="https://binary.pensoft.net/fig/505189" pageId="0" pageNumber="1">3</figureCitation>
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). The larvae of
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<taxonomicName class="Polychaeta" family="Spionidae" genus="Prionospio" kingdom="Animalia" lsidName="Prionospio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="1">Prionospio</emphasis>
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</taxonomicName>
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sp. 1 and 2 are similar to each other and to that of
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<taxonomicName class="Polychaeta" higherTaxonomySource="GBIF,CoL" kingdom="Animalia" lsidName="P. membranacea" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="membranacea">
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<emphasis italics="true" pageId="0" pageNumber="1">P. membranacea</emphasis>
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</taxonomicName>
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, and it is difficult to distinguish among them based only on their morphology.
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |