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<document id="C2BFCBCB6AE3B41612DD9C103C8E0A54" ID-DOI="10.11646/zootaxa.3741.2.3" ID-GBIF-Dataset="fe1a3ebe-6817-4923-935c-778dc4465d4b" ID-ISSN="1175-5326" ID-Zenodo-Dep="222642" ID-ZooBank="3BEA5254-C4C0-47D9-9C5E-DA0448C3259E" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1460703262441" checkinUser="plazi" docAuthor="Montagna, Matteo, Sassi, Davide &amp; Giorgi, Annamaria" docDate="2013" docId="03E987C3FF92FFFF28D0FD3B3790FE61" docLanguage="en" docName="zt03741p253.pdf" docOrigin="Zootaxa 3741 (2)" docStyle="DocumentStyle:647186512141C8FC8976D5BCC54AEB7D.9:Zootaxa.2013-.journal_article" docStyleId="647186512141C8FC8976D5BCC54AEB7D" docStyleName="Zootaxa.2013-.journal_article" docStyleVersion="9" docTitle="Pachybrachis holerorum Montagna &amp; Sassi, new species" docType="treatment" docVersion="9" lastPageNumber="252" masterDocId="FFD0FFBBFF97FFF62847FFC3332DFFB1" masterDocTitle="Pachybrachis holerorum (Coleoptera: Chrysomelidae: Cryptocephalinae), a new species from the Apennines, Italy, identified by integration of morphological and molecular data" masterLastPageNumber="253" masterPageNumber="243" pageNumber="248" updateTime="1698356668825" updateUser="plazi">
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<mods:title id="E5281C6B4520368AE0EEBBB1FED4BD01">Pachybrachis holerorum (Coleoptera: Chrysomelidae: Cryptocephalinae), a new species from the Apennines, Italy, identified by integration of morphological and molecular data</mods:title>
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<mods:name id="0EAC60B152C0B82CDD08D48B0458EB0B" type="personal">
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<mods:namePart id="A010FAF17022C6AF7A9F396394A833FC">Montagna, Matteo</mods:namePart>
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<mods:namePart id="D045F23B2A93F127992430C98868088D">Sassi, Davide</mods:namePart>
</mods:name>
<mods:name id="F0D4023D1869B2B1DB2B86EB685B35B1" type="personal">
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<mods:namePart id="84A742B315D507EAD7D8A7BB5BC2853F">Giorgi, Annamaria</mods:namePart>
</mods:name>
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<mods:title id="495D232D74690FFAACEFF0D53E94845B">Zootaxa</mods:title>
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<mods:date id="176C909A21334BB97E4F048C85A54ED7">2013</mods:date>
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<mods:number id="0D8EAD1BA73C95E4B0CCE8C5D6DDCA86">3741</mods:number>
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<mods:identifier id="DD6C3984BC29C46A05C9517028AD1C3C" type="DOI">10.11646/zootaxa.3741.2.3</mods:identifier>
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<treatment id="03E987C3FF92FFFF28D0FD3B3790FE61" ID-DOI="http://doi.org/10.5281/zenodo.5691285" ID-GBIF-Taxon="119583579" ID-Zenodo-Dep="5691285" LSID="urn:lsid:plazi:treatment:03E987C3FF92FFFF28D0FD3B3790FE61" httpUri="http://treatment.plazi.org/id/03E987C3FF92FFFF28D0FD3B3790FE61" lastPageId="9" lastPageNumber="252" pageId="5" pageNumber="248">
<subSubSection id="C35A655EFF92FFF328D0FD3B322CFC85" pageId="5" pageNumber="248" type="nomenclature">
<paragraph id="8BFF36D5FF92FFF328D0FD3B3063FCA3" blockId="5.[151,846,760,820]" box="[151,846,760,786]" pageId="5" pageNumber="248">
<heading id="D0B781B9FF92FFF328D0FD3B3063FCA3" bold="true" box="[151,846,760,786]" fontSize="11" level="1" pageId="5" pageNumber="248" reason="1">
<emphasis id="B934EAC7FF92FFF328D0FD3B3063FCA3" bold="true" box="[151,846,760,786]" pageId="5" pageNumber="248">
<taxonomicName id="4C404D56FF92FFF328D0FD3B319FFCA3" authority="Montagna &amp; Sassi" authorityName="Montagna &amp; Sassi" box="[151,690,760,786]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="5" pageNumber="248" phylum="Arthropoda" rank="species" species="holerorum" status="sp. nov.">
<emphasis id="B934EAC7FF92FFF328D0FD3B32E8FCA3" bold="true" box="[151,453,760,786]" italics="true" pageId="5" pageNumber="248">Pachybrachis holerorum</emphasis>
Montagna &amp; Sassi
</taxonomicName>
,
<taxonomicNameLabel id="A20757BCFF92FFF32AF8FD3B3063FCA3" box="[703,846,760,786]" pageId="5" pageNumber="248" rank="species">new species</taxonomicNameLabel>
</emphasis>
</heading>
</paragraph>
<paragraph id="8BFF36D5FF92FFF328D0FCD8322CFC85" blockId="5.[151,846,760,820]" box="[151,257,795,820]" pageId="5" pageNumber="248">(figure 3)</paragraph>
</subSubSection>
<subSubSection id="C35A655EFF92FFF328D0FCA031DCFA9D" pageId="5" pageNumber="248" type="materials_examined">
<paragraph id="8BFF36D5FF92FFF328D0FCA03078FC56" blockId="5.[151,1437,867,2008]" pageId="5" pageNumber="248">
<emphasis id="B934EAC7FF92FFF328D0FCA032A9FCCD" bold="true" box="[151,388,867,892]" pageId="5" pageNumber="248">Material examined.</emphasis>
<emphasis id="B934EAC7FF92FFF329C8FCA032D3FCCA" box="[399,510,867,891]" italics="true" pageId="5" pageNumber="248">
<typeStatus id="54FB8877FF92FFF329C8FCA032D7FCCA" box="[399,506,867,891]" pageId="5" pageNumber="248" type="holotype">Holotype</typeStatus>
.
</emphasis>
Male, deposited in MSNM. Original label: Emilia-Romagna, Parma, Passo del Pellizzone,
<quantity id="4CB89B30FF92FFF32958FC443256FC2E" box="[287,379,903,927]" metricMagnitude="3" metricUnit="m" metricValue="1.0" pageId="5" pageNumber="248" unit="m" value="1000.0">1000 m</quantity>
,
<date id="FFFE1015FF92FFF329CBFC4432D2FC2E" box="[396,511,903,927]" pageId="5" pageNumber="248" value="2011-06-08">8.VI.2011</date>
, Montagna &amp; Sassi leg.,
<geoCoordinate id="EE745012FF92FFF32B76FC4430F5FC2E" box="[817,984,903,927]" direction="north" orientation="latitude" pageId="5" pageNumber="248" precision="1" value="44.680233">44°40'48.84&quot;N</geoCoordinate>
<geoCoordinate id="EE745012FF92FFF32BA3FC4437ABFC2E" box="[996,1158,903,928]" direction="east" orientation="longitude" pageId="5" pageNumber="248" precision="1" value="9.745184">9°44'42.66&quot; E</geoCoordinate>
[white label, printed] /
<taxonomicName id="4C404D56FF92FFF328D0FC68329DFC72" box="[151,432,939,963]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="5" pageNumber="248" phylum="Arthropoda" rank="species" species="holerorum" status="sp. nov.">
<emphasis id="B934EAC7FF92FFF328D0FC68329DFC72" box="[151,432,939,963]" italics="true" pageId="5" pageNumber="248">Pachybrachis holerorum</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A20757BCFF92FFF329FDFC6832D1FC75" box="[442,508,939,964]" pageId="5" pageNumber="248" rank="species">
<emphasis id="B934EAC7FF92FFF329FDFC6832D1FC75" bold="true" box="[442,508,939,964]" pageId="5" pageNumber="248">n. sp.</emphasis>
</taxonomicNameLabel>
<typeStatus id="54FB8877FF92FFF32A40FC683158FC75" box="[519,629,939,964]" pageId="5" pageNumber="248" type="holotype">holotypus</typeStatus>
Montagna &amp; Sassi des. [red, printed]. DNA extracted to perform the amplification through PCRs of the genes:
<emphasis id="B934EAC7FF92FFF32A29FC0C318EFC56" box="[622,675,975,999]" italics="true" pageId="5" pageNumber="248">cox1</emphasis>
,
<emphasis id="B934EAC7FF92FFF32AF6FC0C3063FC56" box="[689,846,975,999]" italics="true" pageId="5" pageNumber="248">rbcL and trnL</emphasis>
.
</paragraph>
<paragraph id="8BFF36D5FF92FFF32880FC3731DCFA9D" blockId="5.[151,1437,867,2008]" pageId="5" pageNumber="248">
<typeStatus id="54FB8877FF92FFF32880FC373266FBBA" box="[199,331,1012,1035]" pageId="5" pageNumber="248" type="paratype">
<emphasis id="B934EAC7FF92FFF32880FC373266FBBA" box="[199,331,1012,1035]" italics="true" pageId="5" pageNumber="248">Paratypes</emphasis>
</typeStatus>
.
<specimenCount id="9D46FD5CFF92FFF32918FC303122FBBD" box="[351,527,1011,1036]" pageId="5" pageNumber="248" type="generic">46 specimens</specimenCount>
: Emilia-Romagna, Parma, Passo del Pellizzone,
<quantity id="4CB89B30FF92FFF32C71FC3737A1FBBA" box="[1078,1164,1012,1036]" metricMagnitude="3" metricUnit="m" metricValue="1.0" pageId="5" pageNumber="248" unit="m" value="1000.0">1000 m</quantity>
, Montagna &amp; Sassi leg.,
<geoCoordinate id="EE745012FF92FFF328D0FBD43213FB9E" box="[151,318,1047,1071]" direction="north" orientation="latitude" pageId="5" pageNumber="248" precision="1" value="44.680233">44°40'48.84&quot;N</geoCoordinate>
<geoCoordinate id="EE745012FF92FFF32903FBD432CCFB9E" box="[324,481,1047,1072]" direction="east" orientation="longitude" pageId="5" pageNumber="248" precision="1" value="9.745184">9°44'42.66&quot; E</geoCoordinate>
[white labels, printed],
<specimenCount id="9D46FD5CFF92FFF32AACFBD43020FB9E" box="[747,781,1047,1071]" pageId="5" pageNumber="248" type="generic">8♂</specimenCount>
and 19 ♀, DNA extracted from
<specimenCount id="9D46FD5CFF92FFF32CF7FBD437F8FB9E" box="[1200,1237,1047,1071]" pageId="5" pageNumber="248" type="generic">4♂</specimenCount>
(gene
<emphasis id="B934EAC7FF92FFF32D62FBD43676FB9E" box="[1317,1371,1047,1071]" italics="true" pageId="5" pageNumber="248">cox1</emphasis>
) and 2♀ (genes:
<emphasis id="B934EAC7FF92FFF32963FBF83277FBE2" box="[292,346,1083,1107]" italics="true" pageId="5" pageNumber="248">cox1</emphasis>
,
<emphasis id="B934EAC7FF92FFF3292EFBF83127FBE2" box="[361,522,1083,1107]" italics="true" pageId="5" pageNumber="248">rbcL and trnL</emphasis>
); Lombardia, PV, dint. Brallo di Pregola, Cima Colletta,
<quantity id="4CB89B30FF92FFF32CDAFBF837DBFBE2" box="[1181,1270,1083,1108]" metricMagnitude="3" metricUnit="m" metricValue="1.366" pageId="5" pageNumber="248" unit="m" value="1366.0">1366 m</quantity>
,
<date id="FFFE1015FF92FFF32D43FBF836BAFBE5" box="[1284,1431,1083,1108]" pageId="5" pageNumber="248" value="2011-06-16">16. VI. 2011</date>
, Sassi leg.,
<geoCoordinate id="EE745012FF92FFF32955FB9C3295FBC9" box="[274,440,1119,1144]" direction="north" orientation="latitude" pageId="5" pageNumber="248" precision="1" value="44.71044">44°42'37.59&quot;N</geoCoordinate>
<geoCoordinate id="EE745012FF92FFF32986FB9C317AFBC9" box="[449,599,1119,1144]" direction="east" orientation="longitude" pageId="5" pageNumber="248" precision="1" value="9.260153">9°15'36.55&quot;E</geoCoordinate>
[white labels, printed],
<specimenCount id="9D46FD5CFF92FFF32B2FFB9C30A6FBC6" box="[872,907,1119,1143]" pageId="5" pageNumber="248" type="generic">3♂</specimenCount>
and 1♀; Em. Romagna, MO, Nirano, Salse di Nirano,
<date id="FFFE1015FF92FFF328BFFB473243FB2D" box="[248,366,1156,1180]" pageId="5" pageNumber="248" value="2007-05-30">30.V.2007</date>
, D. Sassi leg. [white label, handwritten],
<specimenCount id="9D46FD5CFF92FFF32B23FB4730ABFB2D" box="[868,902,1156,1180]" pageId="5" pageNumber="248" type="generic">1♂</specimenCount>
and 2 ♀; LOMB. Emilia, Lago di Trebecco,
<date id="FFFE1015FF92FFF328D0FB643224FB71" box="[151,265,1191,1216]" pageId="5" pageNumber="248" value="1997-11-06">11.6.1997</date>
, leg. D. Sassi, [white label, printed],
<specimenCount id="9D46FD5CFF92FFF32AE1FB6431E1FB0E" box="[678,716,1191,1215]" pageId="5" pageNumber="248" type="generic">2♂</specimenCount>
; Lombardia, PV, R, de' Giorgi,
<date id="FFFE1015FF92FFF32C6CFB6437B3FB71" box="[1067,1182,1191,1216]" pageId="5" pageNumber="248" value="1990-06-16">16.6.1990</date>
, D. Sassi,
<specimenCount id="9D46FD5CFF92FFF32D55FB643618FB0E" box="[1298,1333,1191,1215]" pageId="5" pageNumber="248" type="generic">3♂</specimenCount>
and 6 ♀ [white label, printed]; Em. Romagna, MO, Sassatella,
<quantity id="4CB89B30FF92FFF32AB9FB0F3065FB52" box="[766,840,1228,1252]" metricMagnitude="2" metricUnit="m" metricValue="8.0" pageId="5" pageNumber="248" unit="m" value="800.0">800 m</quantity>
,
<date id="FFFE1015FF92FFF32B13FB0F30E7FB55" box="[852,970,1228,1252]" pageId="5" pageNumber="248" value="2007-05-30">30.V.2007</date>
, D. Sassi leg., [white label, printed],
<specimenCount id="9D46FD5CFF92FFF32D37FB0F36B8FB55" box="[1392,1429,1228,1252]" pageId="5" pageNumber="248" type="generic">1♂</specimenCount>
. All
<typeStatus id="54FB8877FF92FFF32881FB33321CFAB9" box="[198,305,1264,1288]" pageId="5" pageNumber="248" type="paratype">paratypes</typeStatus>
with our label:
<taxonomicName id="4C404D56FF92FFF329B3FB2C3022FAB6" box="[500,783,1263,1287]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="5" pageNumber="248" phylum="Arthropoda" rank="species" species="holerorum" status="sp. nov.">
<emphasis id="B934EAC7FF92FFF329B3FB2C3022FAB6" box="[500,783,1263,1287]" italics="true" pageId="5" pageNumber="248">Pachybrachis holerorum</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A20757BCFF92FFF32B5CFB2C3073FAB9" box="[795,862,1263,1288]" pageId="5" pageNumber="248" rank="species">
<emphasis id="B934EAC7FF92FFF32B5CFB2C3073FAB9" bold="true" box="[795,862,1263,1288]" pageId="5" pageNumber="248">n. sp.</emphasis>
</taxonomicNameLabel>
<typeStatus id="54FB8877FF92FFF32B2EFB3330FBFAB9" box="[873,982,1264,1288]" pageId="5" pageNumber="248" type="paratype">paratypus</typeStatus>
Montagna &amp; Sassi des. [red, printed].
<typeStatus id="54FB8877FF92FFF328D0FAD73229FA9D" box="[151,260,1300,1324]" pageId="5" pageNumber="248" type="paratype">Paratypes</typeStatus>
in MSNM, DSPC, MMPC, MSPC, MDPC.
</paragraph>
</subSubSection>
<subSubSection id="C35A655EFF92FFF32880FAF433F5FAC5" pageId="5" pageNumber="248" type="etymology">
<paragraph id="8BFF36D5FF92FFF32880FAF433F5FAC5" blockId="5.[151,1437,867,2008]" pageId="5" pageNumber="248">
<emphasis id="B934EAC7FF92FFF32880FAF43263FAE1" bold="true" box="[199,334,1335,1360]" pageId="5" pageNumber="248">Etymology.</emphasis>
The name is genitive plural of
<emphasis id="B934EAC7FF92FFF32A87FAF431D0FAFE" box="[704,765,1335,1359]" italics="true" pageId="5" pageNumber="248">holus</emphasis>
, used by the Roman poet Lucilius to indicate leguminous forbs.
</paragraph>
</subSubSection>
<subSubSection id="C35A655EFF92FFF12880FABC3166FF64" lastPageId="7" lastPageNumber="250" pageId="5" pageNumber="248" type="description">
<paragraph id="8BFF36D5FF92FFF32880FABC31E2F9B5" blockId="5.[151,1437,867,2008]" pageId="5" pageNumber="248">
<emphasis id="B934EAC7FF92FFF32880FABC3293FA29" bold="true" box="[199,446,1407,1432]" pageId="5" pageNumber="248">Description of male.</emphasis>
Total length: male = 2.9±
<quantity id="4CB89B30FF92FFF32AB6FABC3060FA26" box="[753,845,1407,1431]" metricMagnitude="-4" metricUnit="m" metricValue="1.0" pageId="5" pageNumber="248" unit="mm" value="0.1">0.1 mm</quantity>
. Head yellow except vertex, a median longitudinal stripe with bifurcated apex along frons, antennae sockets, anterior margin of clypeus black. Frons shining, covered with fairly impressed punctation, denser on clypeus and above insertion of antennae, sparser on frons. Antennae filiform, brownish, segments 15 partly yellowish.
</paragraph>
<paragraph id="8BFF36D5FF92FFF32880F9D332A8F869" blockId="5.[151,1437,867,2008]" pageId="5" pageNumber="248">Pronotum black with yellow bands along anterior and lateral borders; anterior band lightly thickened at anterior angles and with short median posteriorly directed vitta; two anteriorly directed yellow lines from basal margin at sides of scutellum, 1.5 times wider than long, regularly curved at sides, with maximum width at about middle; punctation deep, denser at sides, slightly sparser on disc. Scutellum elevated, black, minutely punctate, apically truncate. Elytra coarsely punctured, partially arranged in striae; interstices raised, black with yellow pattern slightly raised from black surface, arranged as follows (spots and vittae may be interrupted or absent): narrow bands and vittae along anterior, lateral and posterior margins; narrow vitta along posterior half of sutural margin; two elongated spots near suture, plus one behind scutellum and one larger, in median position; longitudinal vitta on anterior margin lateral to humeral callus; post-median spot on disc; several smaller spots variously arranged on elytral surface. Epipleura black in posterior half, partly yellow anteriorly, with one or two series of irregularly aligned punctures on edges. Venter black, mesepimera with yellow spot, sometimes indistinct. Abdominal ventrites sparsely punctured and covered with rather sparse, long whitish hairs; ventrite 5 with shallow depression, glabrous.</paragraph>
<caption id="DF3F665DFF91FFF028D0FCBC30B2FC24" box="[151,927,895,917]" httpUri="https://zenodo.org/record/222645/files/figure.png" pageId="6" pageNumber="249" targetBox="[151,1436,193,872]" targetPageId="6">
<paragraph id="8BFF36D5FF91FFF028D0FCBC30B2FC24" blockId="6.[151,927,895,917]" box="[151,927,895,917]" pageId="6" pageNumber="249">
<emphasis id="B934EAC7FF91FFF028D0FCBC3221FC25" bold="true" box="[151,268,895,917]" pageId="6" pageNumber="249">FIGURE 3</emphasis>
.
<taxonomicName id="4C404D56FF91FFF0295EFCBC31F5FC24" authority="Montagna &amp; Sassi" authorityName="Montagna &amp; Sassi" box="[281,728,895,917]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="6" pageNumber="249" phylum="Arthropoda" rank="species" species="holerorum">
<emphasis id="B934EAC7FF91FFF0295EFCBC3139FC25" box="[281,532,895,916]" italics="true" pageId="6" pageNumber="249">Pachybrachis holerorum</emphasis>
Montagna &amp; Sassi
</taxonomicName>
, dorso-lateral view.
</paragraph>
</caption>
<caption id="DF3F665DFF91FFF028D0FA2231FCF9A4" httpUri="https://zenodo.org/record/222646/files/figure.png" pageId="6" pageNumber="249" targetBox="[398,1189,966,1483]" targetPageId="6">
<paragraph id="8BFF36D5FF91FFF028D0FA2231FCF9A4" blockId="6.[151,1436,1505,1557]" pageId="6" pageNumber="249">
<emphasis id="B934EAC7FF91FFF028D0FA223238FA47" bold="true" box="[151,277,1505,1527]" pageId="6" pageNumber="249">FIGURE 4</emphasis>
.
<taxonomicName id="4C404D56FF91FFF0296EFA223024FA46" authority="Montagna &amp; Sassi" authorityName="Montagna &amp; Sassi" box="[297,777,1505,1527]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="6" pageNumber="249" phylum="Arthropoda" rank="species" species="holerorum">
<emphasis id="B934EAC7FF91FFF0296EFA223100FA47" box="[297,557,1505,1526]" italics="true" pageId="6" pageNumber="249">Pachybrachis holerorum</emphasis>
Montagna &amp; Sassi
</taxonomicName>
, holotype, median lobe of aedeagus, scale bar = 100 µm, magnification 130x.
<emphasis id="B934EAC7FF91FFF0292EF9C33258F9A4" bold="true" box="[361,373,1536,1557]" pageId="6" pageNumber="249">a</emphasis>
: dorsal,
<emphasis id="B934EAC7FF91FFF0298AF9C332F6F9A4" bold="true" box="[461,475,1536,1557]" pageId="6" pageNumber="249">b</emphasis>
: ventral,
<emphasis id="B934EAC7FF91FFF02A7BF9C3316AF9A4" bold="true" box="[572,583,1536,1557]" pageId="6" pageNumber="249">c</emphasis>
: lateral view.
</paragraph>
</caption>
<paragraph id="8BFF36D5FF91FFF02880F98030E9F912" blockId="6.[151,1436,1602,2023]" pageId="6" pageNumber="249">Legs yellow, fore femora blackish along posterior edge; median and hind femora largely darkened along basal half, fore tibiae yellow; meso and metatibiae darkened at apex; tarsi mostly yellow, more or less darkened towards apex. First protarsomere moderately broadened, as wide as apex of tibia.</paragraph>
<paragraph id="8BFF36D5FF91FFF02880F96C3733F95A" blockId="6.[151,1436,1602,2023]" pageId="6" pageNumber="249">Apex of aedeagal median lobe (figure 4) acute, lateroventrally with row of white hairs, shaft thin and elongated, slightly careened along ventral surface, venter straight in lateral view.</paragraph>
<paragraph id="8BFF36D5FF91FFF12880F9353166FF64" blockId="6.[151,1436,1602,2023]" lastBlockId="7.[151,1437,151,608]" lastPageId="7" lastPageNumber="250" pageId="6" pageNumber="249">
Female differs from male in: larger and stouter body (length 3.3±
<quantity id="4CB89B30FF91FFF02BF4F9343723F8BE" box="[947,1038,1783,1807]" metricMagnitude="-4" metricUnit="m" metricValue="1.0" pageId="6" pageNumber="249" unit="mm" value="0.1">0.1 mm</quantity>
); frons broader and, as result, eyes more separated; generally reduced yellow pattern; first protarsomeres significantly narrower than tibiae apically; rectal apparatus (figure 5a,b) with two dorsal and one ventral sclerites; dorsal sclerites short and narrow, slightly wider than rectum, transverse connection across dorsal fold not perceptible, so sclerites essentially reduced to the apodemes only; ventral sclerite ribbon-like, evenly pigmented in the middle, with large and rounded apodemes at both ends, wider than rectum; dorsal and ventral sclerotizations of lateral fold present; spermatheca (figure 5c) sickle-shaped, lightly pigmented, basal part not swollen; base reflexed, with gland and duct insertions well sclerotized, so that it seems bifurcated; duct not coiled, quite short, its insertion on bursa copulatrix not enlarged and only feebly and briefly pigmented.
</paragraph>
</subSubSection>
<subSubSection id="C35A655EFF90FFF12880FF1C3398FDA9" pageId="7" pageNumber="250" type="diagnosis">
<paragraph id="8BFF36D5FF90FFF12880FF1C3398FDA9" blockId="7.[151,1437,151,608]" pageId="7" pageNumber="250">
<emphasis id="B934EAC7FF90FFF12880FF1C326CFF49" bold="true" box="[199,321,223,248]" pageId="7" pageNumber="250">Diagnosis.</emphasis>
<taxonomicName id="4C404D56FF90FFF1290DFF2332C9FF49" box="[330,484,224,248]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="7" pageNumber="250" phylum="Arthropoda" rank="genus">
<emphasis id="B934EAC7FF90FFF1290DFF2332C9FF49" box="[330,484,224,248]" italics="true" pageId="7" pageNumber="250">Pachybrachis</emphasis>
</taxonomicName>
of medium-small size, characterized by elytral pattern, with marginal yellow stripe from anterior edge, to and around posterior margin, then along apical half of suture. This stripe interrupted only at humeral callus. Similar elytral marginal stripes are present in
<taxonomicName id="4C404D56FF90FFF12B0BFEEA3733FEF1" authority="Weise" authorityName="Weise" box="[844,1054,295,320]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="7" pageNumber="250" phylum="Arthropoda" rank="species" species="karamani">
<emphasis id="B934EAC7FF90FFF12B0BFEEA30FFFEF1" box="[844,978,296,320]" italics="true" pageId="7" pageNumber="250">P. karamani</emphasis>
Weise
</taxonomicName>
and
<taxonomicName id="4C404D56FF90FFF12C13FEEA3672FEF1" authority="Suffrian" authorityName="Suffrian" box="[1108,1375,295,320]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="7" pageNumber="250" phylum="Arthropoda" rank="species" species="fimbriolatus">
<emphasis id="B934EAC7FF90FFF12C13FEEA37D4FEF1" box="[1108,1273,296,320]" italics="true" pageId="7" pageNumber="250">P. fimbriolatus</emphasis>
Suffrian
</taxonomicName>
, with which the new species forms a group, based on morphology. In
<taxonomicName id="4C404D56FF90FFF12B20FE8E30C3FED5" box="[871,1006,332,356]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="7" pageNumber="250" phylum="Arthropoda" rank="species" species="karamani">
<emphasis id="B934EAC7FF90FFF12B20FE8E30C3FED5" box="[871,1006,332,356]" italics="true" pageId="7" pageNumber="250">P. karamani</emphasis>
</taxonomicName>
, the elytral yellow spots are generally smaller, and the irregularly distributed small spots that characterize
<taxonomicName id="4C404D56FF90FFF12BFFFEB23760FE39" box="[952,1101,368,392]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="7" pageNumber="250" phylum="Arthropoda" rank="species" species="holerorum">
<emphasis id="B934EAC7FF90FFF12BFFFEB23760FE39" box="[952,1101,368,392]" italics="true" pageId="7" pageNumber="250">P. holerorum</emphasis>
</taxonomicName>
are almost entirely missing.
<taxonomicName id="4C404D56FF90FFF128D0FE5732EDFE1D" box="[151,448,404,428]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="7" pageNumber="250" phylum="Arthropoda" rank="species" species="fimbriolatus">
<emphasis id="B934EAC7FF90FFF128D0FE5732EDFE1D" box="[151,448,404,428]" italics="true" pageId="7" pageNumber="250">Pachybrachis fimbriolatus</emphasis>
</taxonomicName>
is distinguished from the new species by: less transverse and more minutely and densely punctured pronotum, reduced elytral yellow spotting, particularly, postmedian dot nearly always absent. The new species clearly differs from the all
<taxonomicName id="4C404D56FF90FFF12A5DFE1F3199FE45" box="[538,692,476,500]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="7" pageNumber="250" phylum="Arthropoda" rank="genus">
<emphasis id="B934EAC7FF90FFF12A5DFE1F3199FE45" box="[538,692,476,500]" italics="true" pageId="7" pageNumber="250">Pachybrachis</emphasis>
</taxonomicName>
species in the shape of the median lobe of aedeagus (figures 4 and 6).
</paragraph>
</subSubSection>
<subSubSection id="C35A655EFF90FFF12880FDE73790FDD1" pageId="7" pageNumber="250" type="distribution">
<paragraph id="8BFF36D5FF90FFF12880FDE73790FDD1" blockId="7.[151,1437,151,608]" pageId="7" pageNumber="250">
<emphasis id="B934EAC7FF90FFF12880FDE7324DFD8C" bold="true" box="[199,352,548,573]" pageId="7" pageNumber="250">Distribution.</emphasis>
The new species is endemic to Northern Apennines,
<collectingCountry id="F3577645FF90FFF12BFFFDE730C0FD8C" box="[952,1005,548,573]" name="Italy" pageId="7" pageNumber="250">Italy</collectingCountry>
. The
<typeStatus id="54FB8877FF90FFF12C6CFDE63776FD8C" box="[1067,1115,549,573]" pageId="7" pageNumber="250">type</typeStatus>
locality is Emilia Romagna, Piacenza Prov., Passo del Pellizzone (also written “Pelizzone”) (
<geoCoordinate id="EE745012FF90FFF12B20FD8B3722FDD1" box="[871,1039,584,608]" direction="north" orientation="latitude" pageId="7" pageNumber="250" precision="1" value="44.680233">44°40'48.84&quot;N</geoCoordinate>
<geoCoordinate id="EE745012FF90FFF12C51FD84379CFDD1" box="[1046,1201,583,608]" direction="east" orientation="longitude" pageId="7" pageNumber="250" precision="1" value="9.745184">9°44'42.66&quot;E</geoCoordinate>
).
</paragraph>
</subSubSection>
<caption id="DF3F665DFF90FFF128D0F8A53666F8CD" box="[151,1355,1894,1916]" httpUri="https://zenodo.org/record/222647/files/figure.png" pageId="7" pageNumber="250" targetBox="[167,1420,670,1859]" targetPageId="7">
<paragraph id="8BFF36D5FF90FFF128D0F8A53666F8CD" blockId="7.[151,1355,1894,1916]" box="[151,1355,1894,1916]" pageId="7" pageNumber="250">
<emphasis id="B934EAC7FF90FFF128D0F8A53221F8CD" bold="true" box="[151,268,1894,1916]" pageId="7" pageNumber="250">FIGURE 5</emphasis>
.
<taxonomicName id="4C404D56FF90FFF1295EF8A431F5F8CD" authority="Montagna &amp; Sassi" authorityName="Montagna &amp; Sassi" box="[281,728,1894,1916]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="7" pageNumber="250" phylum="Arthropoda" rank="species" species="holerorum">
<emphasis id="B934EAC7FF90FFF1295EF8A43139F8CD" box="[281,532,1895,1916]" italics="true" pageId="7" pageNumber="250">Pachybrachis holerorum</emphasis>
Montagna &amp; Sassi
</taxonomicName>
, female rectal apparatus.
<emphasis id="B934EAC7FF90FFF12B9DF8A430CAF8CD" bold="true" box="[986,999,1895,1916]" pageId="7" pageNumber="250">a</emphasis>
: ventral,
<emphasis id="B934EAC7FF90FFF12C00F8A43778F8CD" bold="true" box="[1095,1109,1895,1916]" pageId="7" pageNumber="250">b</emphasis>
: dorsal,
<emphasis id="B934EAC7FF90FFF12CEBF8A4379AF8CD" bold="true" box="[1196,1207,1895,1916]" pageId="7" pageNumber="250">c</emphasis>
: spermatheca.
</paragraph>
</caption>
<caption id="DF3F665DFF9FFFFE28D0FD0A32C8FD4F" httpUri="https://zenodo.org/record/222648/files/figure.png" pageId="8" pageNumber="251" targetBox="[397,1189,193,692]" targetPageId="8">
<paragraph id="8BFF36D5FF9FFFFE28D0FD0A32C8FD4F" blockId="8.[151,1436,713,766]" pageId="8" pageNumber="251">
<emphasis id="B934EAC7FF9FFFFE28D0FD0A3223FD6F" bold="true" box="[151,270,713,735]" pageId="8" pageNumber="251">FIGURE 6</emphasis>
.
<taxonomicName id="4C404D56FF9FFFFE295BFD093175FD6E" authority="Weise" authorityName="Weise" box="[284,600,713,735]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="species" species="karamani">
<emphasis id="B934EAC7FF9FFFFE295BFD09313DFD6E" box="[284,528,714,735]" italics="true" pageId="8" pageNumber="251">Pachybrachis karamani</emphasis>
Weise
</taxonomicName>
, lectotype, median lobe of aedeagus, scale bar = 100 µm, magnification 140x.
<emphasis id="B934EAC7FF9FFFFE2DCFFD0936B8FD6E" bold="true" box="[1416,1429,714,735]" pageId="8" pageNumber="251">a</emphasis>
: dorsal,
<emphasis id="B934EAC7FF9FFFFE28A6FD2B33C2FD4C" bold="true" box="[225,239,744,765]" pageId="8" pageNumber="251">b</emphasis>
: ventral,
<emphasis id="B934EAC7FF9FFFFE2917FD2B3276FD4C" bold="true" box="[336,347,744,765]" pageId="8" pageNumber="251">c</emphasis>
: lateral view.
</paragraph>
</caption>
<subSubSection id="C35A655EFF9FFFFF2880FCE93790FE61" lastPageId="9" lastPageNumber="252" pageId="8" pageNumber="251" type="discussion">
<paragraph id="8BFF36D5FF9FFFFE2880FCE93252FC1E" blockId="8.[151,1437,810,1555]" pageId="8" pageNumber="251">
<emphasis id="B934EAC7FF9FFFFE2880FCE93216FCF2" bold="true" box="[199,315,810,835]" pageId="8" pageNumber="251">Remarks.</emphasis>
The biology of
<taxonomicName id="4C404D56FF9FFFFE29A8FCE83152FCF2" box="[495,639,811,835]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="species" species="holerorum">
<emphasis id="B934EAC7FF9FFFFE29A8FCE83152FCF2" box="[495,639,811,835]" italics="true" pageId="8" pageNumber="251">P. holerorum</emphasis>
</taxonomicName>
is poorly known. At Passo del Pellizzone it was collected in early June on
<taxonomicName id="4C404D56FF9FFFFE28FBFC93325BFCD6" box="[188,374,847,871]" class="Magnoliopsida" family="Fabaceae" genus="Lotus" kingdom="Plantae" order="Fabales" pageId="8" pageNumber="251" phylum="Tracheophyta" rank="species" species="herbaceus">
<emphasis id="B934EAC7FF9FFFFE28FBFC93325BFCD6" box="[188,374,847,871]" italics="true" pageId="8" pageNumber="251">Lotus herbaceus</emphasis>
</taxonomicName>
. This possible host was confirmed by the gut analysis of plant DNA from specimens at this locality. The preference of many species of the genus
<taxonomicName id="4C404D56FF9FFFFE2B5BFCB0309BFC3A" box="[796,950,883,907]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="genus">
<emphasis id="B934EAC7FF9FFFFE2B5BFCB0309BFC3A" box="[796,950,883,907]" italics="true" pageId="8" pageNumber="251">Pachybrachis</emphasis>
</taxonomicName>
for
<taxonomicName id="4C404D56FF9FFFFE2BA9FCB1377BFC3A" box="[1006,1110,882,907]" class="Magnoliopsida" family="Fabaceae" kingdom="Plantae" order="Fabales" pageId="8" pageNumber="251" phylum="Tracheophyta" rank="family">Fabaceae</taxonomicName>
is well known (Jolivet and Hawkeswood 1995).
</paragraph>
<paragraph id="8BFF36D5FF9FFFFE2880FC78318EFB8E" blockId="8.[151,1437,810,1555]" pageId="8" pageNumber="251">
<taxonomicName id="4C404D56FF9FFFFE2880FC7832CCFC62" box="[199,481,955,979]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="species" species="holerorum">
<emphasis id="B934EAC7FF9FFFFE2880FC7832CCFC62" box="[199,481,955,979]" italics="true" pageId="8" pageNumber="251">Pachybrachis holerorum</emphasis>
</taxonomicName>
is restricted to the north and west Apennines and
<emphasis id="B934EAC7FF9FFFFE2C7EFC7837EDFC63" box="[1081,1216,955,979]" italics="true" pageId="8" pageNumber="251">P. k a r a m a n i</emphasis>
is on the Adriatic slopes of North and Central Apennines (Sassi 2006). Molecular and morphological evidence show that
<taxonomicName id="4C404D56FF9FFFFE28D0FBC03299FBAA" box="[151,436,1027,1051]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="species" species="holerorum">
<emphasis id="B934EAC7FF9FFFFE28D0FBC03299FBAA" box="[151,436,1027,1051]" italics="true" pageId="8" pageNumber="251">Pachybrachis holerorum</emphasis>
</taxonomicName>
and
<taxonomicName id="4C404D56FF9FFFFE29BEFBC031ABFBAA" box="[505,646,1027,1051]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="species" species="karamani">
<emphasis id="B934EAC7FF9FFFFE29BEFBC031ABFBAA" box="[505,646,1027,1051]" italics="true" pageId="8" pageNumber="251">P. karamani</emphasis>
</taxonomicName>
are recently diverged sister species in adjacent allopatric ranges, suggesting vicariant origin for the two species.
</paragraph>
<paragraph id="8BFF36D5FF9FFFFE2880FB8830AEF9A2" blockId="8.[151,1437,810,1555]" pageId="8" pageNumber="251">
We take the opportunity here to designate a
<typeStatus id="54FB8877FF9FFFFE2AFFFB893032FBD2" box="[696,799,1098,1123]" pageId="8" pageNumber="251" type="lectotype">lectotype</typeStatus>
for
<taxonomicName id="4C404D56FF9FFFFE2B08FB8830FBFBD2" box="[847,982,1099,1123]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="species" species="karamani">
<emphasis id="B934EAC7FF9FFFFE2B08FB8830FBFBD2" box="[847,982,1099,1123]" italics="true" pageId="8" pageNumber="251">P. karamani</emphasis>
</taxonomicName>
, to fix the identity of this species which is similar to
<taxonomicName id="4C404D56FF9FFFFE2961FBB33295FB36" box="[294,440,1135,1159]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="species" species="holerorum">
<emphasis id="B934EAC7FF9FFFFE2961FBB33295FB36" box="[294,440,1135,1159]" italics="true" pageId="8" pageNumber="251">P. holerorum</emphasis>
</taxonomicName>
. The syntypic series of
<emphasis id="B934EAC7FF9FFFFE2A8DFBB33063FB36" box="[714,846,1135,1159]" italics="true" pageId="8" pageNumber="251">P. k a r a m a n i</emphasis>
consists of five specimens. We designate a male as
<typeStatus id="54FB8877FF9FFFFE28D0FB51322CFB1A" box="[151,257,1170,1195]" pageId="8" pageNumber="251" type="lectotype">lectotype</typeStatus>
, labelled as follows: Spalato (handwritten, white label) /
<typeStatus id="54FB8877FF9FFFFE2BEAFB5030DCFB1A" box="[941,1009,1171,1195]" pageId="8" pageNumber="251">Typus</typeStatus>
(printed, red label) /
<taxonomicName id="4C404D56FF9FFFFE2CBFFB503649FB1A" box="[1272,1380,1171,1195]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="species" species="karamani">
<emphasis id="B934EAC7FF9FFFFE2CBFFB503649FB1A" box="[1272,1380,1171,1195]" italics="true" pageId="8" pageNumber="251">karamani</emphasis>
</taxonomicName>
Ws. (handwritten, white label) / Zool. Mus. Berlin (printed, yellow label) / Sintypus (printed, red label) /
<taxonomicName id="4C404D56FF9FFFFE2D45FB74328DFB42" authority="Weise, 1893" authorityName="Weise" authorityYear="1893" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="species" species="karamani">
<emphasis id="B934EAC7FF9FFFFE2D45FB74322FFB42" italics="true" pageId="8" pageNumber="251">Pachybrachis karamani</emphasis>
Weise, 1893
</taxonomicName>
labelled by MNHUB 2012 (printed, red label).
<taxonomicName id="4C404D56FF9FFFFE2BACFB1836B1FB42" authority="Weise, 1893" authorityName="Weise" authorityYear="1893" box="[1003,1436,1242,1267]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="species" species="karamani">
<emphasis id="B934EAC7FF9FFFFE2BACFB1837D3FB42" box="[1003,1278,1243,1267]" italics="true" pageId="8" pageNumber="251">Pachybrachis karamani</emphasis>
Weise, 1893
</taxonomicName>
<typeStatus id="54FB8877FF9FFFFE28D0FB3C3267FAA6" box="[151,330,1279,1303]" pageId="8" pageNumber="251" type="lectotype">LECTOTYPUS</typeStatus>
Montagna &amp; Sassi des. (Printed / red label). The remaining
<typeStatus id="54FB8877FF9FFFFE2BA8FB3D37BCFAA6" box="[1007,1169,1278,1303]" pageId="8" pageNumber="251" type="paralectotype">paralectotypes</typeStatus>
are labelled as follows:
<specimenCount id="9D46FD5CFF9FFFFE28D0FAE033EEFA8B" box="[151,195,1315,1339]" pageId="8" pageNumber="251" type="generic">2 ♂</specimenCount>
, 1 ♀: Spalato (handwritten, white label) /
<typeStatus id="54FB8877FF9FFFFE2AE6FAE031C8FA8A" box="[673,741,1315,1339]" pageId="8" pageNumber="251">Typus</typeStatus>
(printed, red label) / Zool. Mus. Berlin (printed, yellow label) / Sintypus (printed, red label) /
<taxonomicName id="4C404D56FF9FFFFE29B4FA8430A3FAEE" authority="Weise, 1893" authorityName="Weise" authorityYear="1893" box="[499,910,1350,1375]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="species" species="karamani">
<emphasis id="B934EAC7FF9FFFFE29B4FA8431D3FAEE" box="[499,766,1351,1375]" italics="true" pageId="8" pageNumber="251">Pachybrachis karamani</emphasis>
Weise, 1893
</taxonomicName>
labelled by MNHUB 2012 (printed, red label).
<taxonomicName id="4C404D56FF9FFFFE28D0FAA83115FA32" authority="Weise, 1893" authorityName="Weise" authorityYear="1893" box="[151,568,1386,1411]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="species" species="karamani">
<emphasis id="B934EAC7FF9FFFFE28D0FAA83289FA32" box="[151,420,1387,1411]" italics="true" pageId="8" pageNumber="251">Pachybrachis karamani</emphasis>
Weise, 1893
</taxonomicName>
<typeStatus id="54FB8877FF9FFFFE2A06FAA83016FA32" box="[577,827,1387,1411]" pageId="8" pageNumber="251" type="paralectotype">PARALECTOTYPUS</typeStatus>
Montagna &amp; Sassi des. (Printed / red label). Female: Spalato Karam (handwritten, white label) /
<taxonomicName id="4C404D56FF9FFFFE2ACFFA4D31DBFA16" box="[648,758,1422,1447]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="253" phylum="Arthropoda" rank="species" species="karamani">Karamani</taxonomicName>
det. Burlini 1968 (partly printed, white label) / Zool. Mus. Berlin (printed, yellow label) /
<taxonomicName id="4C404D56FF9FFFFE2A44FA71315CFA7A" box="[515,625,1458,1483]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="253" phylum="Arthropoda" rank="species" species="karamani">Karamani</taxonomicName>
(handwritten, white label) /
<typeStatus id="54FB8877FF9FFFFE2B87FA703729FA7A" box="[960,1028,1459,1483]" pageId="8" pageNumber="251">Typus</typeStatus>
(printed, red label) /
<taxonomicName id="4C404D56FF9FFFFE2D45FA70328DFA5E" authority="Weise, 1893" authorityName="Weise" authorityYear="1893" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="species" species="karamani">
<emphasis id="B934EAC7FF9FFFFE2D45FA70322FFA5E" italics="true" pageId="8" pageNumber="251">Pachybrachis karamani</emphasis>
Weise, 1893
</taxonomicName>
labelled by MNHUB 2012 (printed, red label).
<taxonomicName id="4C404D56FF9FFFFE2BACFA1436B1FA5E" authority="Weise, 1893" authorityName="Weise" authorityYear="1893" box="[1003,1436,1494,1519]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="species" species="karamani">
<emphasis id="B934EAC7FF9FFFFE2BACFA1437D3FA5E" box="[1003,1278,1495,1519]" italics="true" pageId="8" pageNumber="251">Pachybrachis karamani</emphasis>
Weise, 1893
</taxonomicName>
<typeStatus id="54FB8877FF9FFFFE28D0FA3832BFF9A2" box="[151,402,1531,1555]" pageId="8" pageNumber="251" type="paralectotype">PARALECTOTYPUS</typeStatus>
Montagna &amp; Sassi des. (Printed / red label).
</paragraph>
<paragraph id="8BFF36D5FF9FFFFE28D0F9A532A1F9CE" blockId="8.[151,396,1638,1663]" box="[151,396,1638,1663]" pageId="8" pageNumber="251">
<emphasis id="B934EAC7FF9FFFFE28D0F9A532A1F9CE" bold="true" box="[151,396,1638,1663]" pageId="8" pageNumber="251">Concluding remarks</emphasis>
</paragraph>
<paragraph id="8BFF36D5FF9FFFFE28D0F97330DBF8BE" blockId="8.[151,1436,1710,1879]" pageId="8" pageNumber="251">
In our study of these
<taxonomicName id="4C404D56FF9FFFFE29DFF96C311FF976" box="[408,562,1711,1735]" class="Insecta" family="Chrysomelidae" genus="Pachybrachis" kingdom="Animalia" order="Coleoptera" pageId="8" pageNumber="251" phylum="Arthropoda" rank="genus">
<emphasis id="B934EAC7FF9FFFFE29DFF96C311FF976" box="[408,562,1711,1735]" italics="true" pageId="8" pageNumber="251">Pachybrachis</emphasis>
</taxonomicName>
populations, the combination of three different approaches (morphological features of the aedeagus, nucleotide distance values and a method delimiting species based on single-locus molecular data), has provided evidence for a new species and its sister taxon.
</paragraph>
<paragraph id="8BFF36D5FF9FFFFE2880F8D930F6F8E6" blockId="8.[151,1436,1710,1879]" pageId="8" pageNumber="251">The results contained in this work strongly confirm the urgent need to increase efforts to uncover the real biodiversity of the European fauna, in particular the Mediterranean region.</paragraph>
<paragraph id="8BFF36D5FF9EFFFF28D0FF543252FF01" blockId="9.[151,383,151,176]" box="[151,383,151,176]" pageId="9" pageNumber="252">
<emphasis id="B934EAC7FF9EFFFF28D0FF543252FF01" bold="true" box="[151,383,151,176]" pageId="9" pageNumber="252">Acknowledgements</emphasis>
</paragraph>
<paragraph id="8BFF36D5FF9EFFFF28D0FF233630FE39" blockId="9.[151,1436,223,464]" pageId="9" pageNumber="252">
We cordially thank Dr. Johannes Frisch and Joachim Willers of the Museum für Naturkunde (Berlin) which give us the opportunity to study the
<typeStatus id="54FB8877FF9EFFFF29A4FEC6313EFEAC" box="[483,531,261,285]" pageId="9" pageNumber="252">type</typeStatus>
series of
<emphasis id="B934EAC7FF9EFFFF2ACFFEC63023FEAD" box="[648,782,260,284]" italics="true" pageId="9" pageNumber="252">P. k a r a m a n i</emphasis>
Weise, 1893. Moreover we thank Dr. Matthias Schöller (Berlin) for the helpful comments, Michele Zilioli of the Museo civico di Storia naturale di Milano for the SEM support, Loris Colacurcio (Bologna) for his precious help during the collecting campaigns, the Editor and two anonymous reviewers for their comments and suggestions to the preliminary version of the manuscript.
</paragraph>
<paragraph id="8BFF36D5FF9EFFFF2880FE573790FE61" blockId="9.[151,1436,223,464]" pageId="9" pageNumber="252">
This work was partially supported by &quot;Accordo di Programma, affermazione in Edolo del Centro di Eccellenza Università della Montagna&quot; MIUR-Università degli Studi di Milano, prot. n. 1293-
<date id="FFFE1015FF9EFFFF2C72FE743794FE61" box="[1077,1209,439,464]" pageId="9" pageNumber="252" value="2011-08-05">05/08/2011</date>
.
</paragraph>
</subSubSection>
</treatment>
</document>