261 lines
44 KiB
XML
261 lines
44 KiB
XML
<document id="11FB4FDFE2C1240555B5A2AAA3521054" ID-DOI="10.5281/zenodo.175835" ID-GBIF-Dataset="b3688ec6-c24b-410e-bed0-8bc91c664938" ID-ISSN="1175-5326" ID-Zenodo-Dep="175835" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1459821648916" checkinUser="plazi" docAuthor="Ereskovsky, Alexander V." docDate="2007" docId="062287A5FFBA6848E4ED4CF50B0BEC39" docLanguage="en" docName="zt01432p066.pdf" docOrigin="Zootaxa 1432" docStyle="DocumentStyle:6581673A57F01A3145754A1E615EDFF0.4:Zootaxa.2007-2008.journal_article" docStyleId="6581673A57F01A3145754A1E615EDFF0" docStyleName="Zootaxa.2007-2008.journal_article" docStyleVersion="4" docTitle="Halisarca korotkovae Ereskovsky, 2007, sp. nov." docType="treatment" docVersion="9" lastPageNumber="65" masterDocId="FA1BFFDDFFBF6840E47A4C620931E84B" masterDocTitle="A new species of Halisarca (Demospongiae: Halisarcida) from the Sea of Okhotsk, North Pacific" masterLastPageNumber="66" masterPageNumber="57" pageNumber="62" updateTime="1698221841595" updateUser="plazi">
|
||
<mods:mods id="AA37061B334BA926A132F2D9F54DCB32" xmlns:mods="http://www.loc.gov/mods/v3">
|
||
<mods:titleInfo id="66C9837A93B79893F4A09827844A58F1">
|
||
<mods:title id="4F7ACAC04023112E8A845B0DE370CA92">A new species of Halisarca (Demospongiae: Halisarcida) from the Sea of Okhotsk, North Pacific</mods:title>
|
||
</mods:titleInfo>
|
||
<mods:name id="FF3998112294F015A2A95BD22905C5D7" type="personal">
|
||
<mods:role id="A9C8630D539E5570237FD6068C3F27C0">
|
||
<mods:roleTerm id="C20F7370CA685C8B82EEF6694B1F97AD">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="15DD85260D556D67FEFEFB3B16807150">Ereskovsky, Alexander V.</mods:namePart>
|
||
</mods:name>
|
||
<mods:typeOfResource id="354B80BCB8107017041E2319397D31FF">text</mods:typeOfResource>
|
||
<mods:relatedItem id="E50A9DABD6E4148DDC0382E650DBE41E" type="host">
|
||
<mods:titleInfo id="2C3ADBE86A7F53CA654DB769AE26D9AD">
|
||
<mods:title id="47A222ABE2934974A17B8B9271A16530">Zootaxa</mods:title>
|
||
</mods:titleInfo>
|
||
<mods:part id="6D6A7A67010087555BDF18A5FD43267D">
|
||
<mods:date id="D5A7C858626B0E4B098D99A7A251EF32">2007</mods:date>
|
||
<mods:detail id="ED622A26DC6E2A9B478527D4526138CB" type="volume">
|
||
<mods:number id="F2DB9084FCCE8BC20984DA01ABCAA4A3">1432</mods:number>
|
||
</mods:detail>
|
||
<mods:extent id="7B417FC8385C1226A14E4D28F0D70463" unit="page">
|
||
<mods:start id="8B1CEDE0C372F750434C70855833F799">57</mods:start>
|
||
<mods:end id="FC1D0D27A12F278492D18BFCAC6F417F">66</mods:end>
|
||
</mods:extent>
|
||
</mods:part>
|
||
</mods:relatedItem>
|
||
<mods:classification id="E41773FA31DEDDE70D7DDBBE5F999832">journal article</mods:classification>
|
||
<mods:identifier id="924979166C35D687E262C8500F3AD4E2" type="DOI">10.5281/zenodo.175835</mods:identifier>
|
||
<mods:identifier id="8150CD0BF853B3FCDF06B67CF43E1AA7" type="GBIF-Dataset">b3688ec6-c24b-410e-bed0-8bc91c664938</mods:identifier>
|
||
<mods:identifier id="4D5D516E96A88866A161A6C8E197C022" type="ISSN">1175-5326</mods:identifier>
|
||
<mods:identifier id="577EEE7B982DCC052B5F6078439C8600" type="Zenodo-Dep">175835</mods:identifier>
|
||
</mods:mods>
|
||
<treatment id="062287A5FFBA6848E4ED4CF50B0BEC39" ID-DOI="http://doi.org/10.5281/zenodo.6238236" ID-GBIF-Taxon="119355807" ID-Zenodo-Dep="6238236" LSID="urn:lsid:plazi:treatment:062287A5FFBA6848E4ED4CF50B0BEC39" httpUri="http://treatment.plazi.org/id/062287A5FFBA6848E4ED4CF50B0BEC39" lastPageId="8" lastPageNumber="65" pageId="5" pageNumber="62">
|
||
<subSubSection id="C6916538FFBA6845E4ED4CF50826E891" pageId="5" pageNumber="62" type="nomenclature">
|
||
<paragraph id="8E3436B3FFBA6845E4ED4CF508CEE8FA" blockId="5.[151,511,151,218]" box="[151,511,151,177]" pageId="5" pageNumber="62">
|
||
<heading id="D57C81DFFFBA6845E4ED4CF508CEE8FA" bold="true" box="[151,511,151,177]" fontSize="11" level="1" pageId="5" pageNumber="62" reason="1">
|
||
<emphasis id="BCFFEAA1FFBA6845E4ED4CF508CEE8FA" bold="true" box="[151,511,151,177]" pageId="5" pageNumber="62">
|
||
<taxonomicName id="498B4D30FFBA6845E4ED4CF508AAE8FA" box="[151,411,151,177]" class="Demospongiae" family="Halisarcidae" genus="Halisarca" kingdom="Animalia" order="Chondrosida" pageId="5" pageNumber="62" phylum="Porifera" rank="species" species="korotkovae" status="sp. nov.">
|
||
<emphasis id="BCFFEAA1FFBA6845E4ED4CF508AAE8FA" bold="true" box="[151,411,151,177]" italics="true" pageId="5" pageNumber="62">Halisarca korotkovae</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A7CC57DAFFBA6845E5D84CF508CEE8FA" box="[418,511,151,177]" pageId="5" pageNumber="62" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="8E3436B3FFBA6845E4ED4CA20826E891" blockId="5.[151,511,151,218]" box="[151,279,192,218]" pageId="5" pageNumber="62">
|
||
(
|
||
<figureCitation id="16B02A36FFBA6845E4E54CA2083EE891" box="[159,271,192,218]" captionStart-0="FIGURE 2" captionStart-1="FIGURE 3" captionStart-2="FIGURE 4" captionStartId-0="5.[151,255,1963,1987]" captionStartId-1="6.[151,251,1745,1769]" captionStartId-2="7.[151,255,1804,1828]" captionTargetBox-0="[333,1267,594,1947]" captionTargetBox-1="[264,1335,194,1731]" captionTargetBox-2="[151,1435,834,1779]" captionTargetId-0="figure@5.[333,1267,594,1947]" captionTargetId-1="figure@6.[264,1335,194,1731]" captionTargetId-2="figure@7.[151,1436,834,1780]" captionTargetPageId-0="5" captionTargetPageId-1="6" captionTargetPageId-2="7" captionText-0="FIGURE 2. External morphology of Halisarca korotkovae sp. nov. A) Photograph of a specimen with even surface. B) Photograph of a type specimen (ZIN RAS 10700) with a slightly undulating surface." captionText-1="FIGURE 3. The anatomical structures of Halisarca korotkovae sp. nov. A) Light microscopy micrograph showing the external part of sponge with the ectosome. Stained with Mayer's hematoxylin-eosin. B) Light microscopy micrograph showing the internal part of sponge with the choanosome. Stained with Heidenhein’s hematoxylin-eosin. Abbreviation: C — cuticle; Cc — choanocyte chamber; Exc — exhalant canal; Mc — microgranular cells; Se — subectosomal cavities." captionText-2="FIGURE 4. Light microscopy micrographs of the cells of Halisarca korotkovae sp. nov. A) Ectosome with cuticle (C), microgranular cells (Mc), exopinacocytes (Ex), amoebocyte (Ac), spherulous cells (Sc), and vacuolar cell (Vc). Stained with Mayer's hematoxylin-eosin. B – D) Choanosome. B) Choanocyte chambers (Cc) consist of choanocytes (Ch), endopinacocytes (En) and spherulous cells (Sc). Stained with Heidenhein’s hematoxylin-eosin. C) Choanosomal mesohyl with vacuolar cells (Vc) and choanocyte chamber (Cc). Stained with Mayer's hematoxylin-eosin. D) Oocytes (O), spherulous cells (Sc) and amoebocyte (Ac) in the choanosome. Stained with Heidenhein’s hematoxylin-eosin." httpUri-0="https://zenodo.org/record/175837/files/figure.png" httpUri-1="https://zenodo.org/record/175838/files/figure.png" httpUri-2="https://zenodo.org/record/175839/files/figure.png" pageId="5" pageNumber="62">Figs. 2–4</figureCitation>
|
||
)
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C6916538FFBA6845E4ED4D6D0BDBEA51" pageId="5" pageNumber="62" type="materials_examined">
|
||
<paragraph id="8E3436B3FFBA6845E4ED4D6D0A0BE919" blockId="5.[151,1437,271,538]" pageId="5" pageNumber="62">
|
||
<emphasis id="BCFFEAA1FFBA6845E4ED4D6D08B7E962" bold="true" box="[151,390,271,297]" italics="true" pageId="5" pageNumber="62">Material examined.</emphasis>
|
||
<typeStatus id="51308811FFBA6845E5F54D7208CDE961" box="[399,508,272,298]" pageId="5" pageNumber="62" type="holotype">Holotype</typeStatus>
|
||
<collectionCode id="E89AAE76FFBA6845E67C4D720B06E961" box="[518,567,272,298]" httpUri="http://grbio.org/cool/z2y8-2jfr" name="Russian Academy of Sciences, Zoological Institute, Zoological Museum" pageId="5" pageNumber="62">ZIN</collectionCode>
|
||
<collectionCode id="E89AAE76FFBA6845E63B4D720B4BE961" box="[577,634,272,298]" pageId="5" pageNumber="62">RAS</collectionCode>
|
||
10700 - (49°57’,7–50°00’,6 N, 145°51’,3–49’,1 E), SW of Sea of Okhotsk,
|
||
<quantity id="49739B56FFBA6845E5734D5A0867E919" box="[265,342,312,338]" metricMagnitude="2" metricUnit="m" metricValue="6.0" pageId="5" pageNumber="62" unit="m" value="600.0">600 m</quantity>
|
||
, 0
|
||
<date id="FA351073FFBA6845E50B4D5A08C4E919" box="[369,501,312,338]" pageId="5" pageNumber="62" value="1984-10-08">8 Oct.1984</date>
|
||
, collector A.V. Ereskovsky.
|
||
</paragraph>
|
||
<paragraph id="8E3436B3FFBA6845E4BF4D020BDBEA51" blockId="5.[151,1437,271,538]" pageId="5" pageNumber="62">
|
||
<typeStatus id="51308811FFBA6845E4BF4D02080AE931" box="[197,315,352,378]" pageId="5" pageNumber="62" type="paratype">Paratypes</typeStatus>
|
||
:
|
||
<collectionCode id="E89AAE76FFBA6845E53C4D020846E931" box="[326,375,352,378]" httpUri="http://grbio.org/cool/z2y8-2jfr" name="Russian Academy of Sciences, Zoological Institute, Zoological Museum" pageId="5" pageNumber="62">ZIN</collectionCode>
|
||
<collectionCode id="E89AAE76FFBA6845E5044D020886E931" box="[382,439,352,378]" pageId="5" pageNumber="62">RAS</collectionCode>
|
||
10701 - (50°02’,5–05’,3 N, 145°32’,2–30’,6 E), SW of Sea of Okhotsk,
|
||
<quantity id="49739B56FFBA6845E16A4D020CA6E931" box="[1296,1431,352,378]" metricMagnitude="2" metricUnit="m" metricValue="4.95" metricValueMax="5.0" metricValueMin="4.9" pageId="5" pageNumber="62" unit="m" value="495.0" valueMax="500.0" valueMin="490.0">490–500 m</quantity>
|
||
, 0
|
||
<date id="FA351073FFBA6845E4DD4DEA081DE9E9" box="[167,300,392,418]" pageId="5" pageNumber="62" value="1984-10-08">8 Oct.1984</date>
|
||
;
|
||
<collectionCode id="E89AAE76FFBA6845E5434DEA085BE9E9" box="[313,362,392,418]" httpUri="http://grbio.org/cool/z2y8-2jfr" name="Russian Academy of Sciences, Zoological Institute, Zoological Museum" pageId="5" pageNumber="62">ZIN</collectionCode>
|
||
<collectionCode id="E89AAE76FFBA6845E5084DEA089AE9E9" box="[370,427,392,418]" pageId="5" pageNumber="62">RAS</collectionCode>
|
||
10702 - (50°59’,2–51°02’,2 N, 145°21’,2–19’,2 E), SW of Sea of Okhotsk,
|
||
<quantity id="49739B56FFBA6845E14C4DEA0980E981" metricMagnitude="2" metricUnit="m" metricValue="6.325" metricValueMax="6.45" metricValueMin="6.2" pageId="5" pageNumber="62" unit="m" value="632.5" valueMax="645.0" valueMin="620.0">620–645 m</quantity>
|
||
,
|
||
<date id="FA351073FFBA6845E4C54DD20864E981" box="[191,341,432,458]" pageId="5" pageNumber="62" value="1984-10-10">10 Oct.1984</date>
|
||
;
|
||
<collectionCode id="E89AAE76FFBA6845E51E4DD208A4E981" box="[356,405,432,458]" httpUri="http://grbio.org/cool/z2y8-2jfr" name="Russian Academy of Sciences, Zoological Institute, Zoological Museum" pageId="5" pageNumber="62">ZIN</collectionCode>
|
||
<collectionCode id="E89AAE76FFBA6845E5E44DD208E6E981" box="[414,471,432,458]" pageId="5" pageNumber="62">RAS</collectionCode>
|
||
10703 - (55°30’,7–30’,7 N, 150°00’,5–08’,4 E), central part of Sea of Okhotsk,
|
||
<quantity id="49739B56FFBA6845E4ED4DBA09D5E9B9" box="[151,228,472,498]" metricMagnitude="2" metricUnit="m" metricValue="3.0" pageId="5" pageNumber="62" unit="m" value="300.0">300 m</quantity>
|
||
,
|
||
<date id="FA351073FFBA6845E4954DBA08B2E9B9" box="[239,387,472,498]" pageId="5" pageNumber="62" value="1984-10-20">20 Oct.1984</date>
|
||
;
|
||
<collectionCode id="E89AAE76FFBA6845E5F54DBA08F1E9B9" box="[399,448,472,498]" httpUri="http://grbio.org/cool/z2y8-2jfr" name="Russian Academy of Sciences, Zoological Institute, Zoological Museum" pageId="5" pageNumber="62">ZIN</collectionCode>
|
||
<collectionCode id="E89AAE76FFBA6845E5BC4DBA08CEE9B9" box="[454,511,472,498]" pageId="5" pageNumber="62">RAS</collectionCode>
|
||
10704 - (55°13',3–10',8 N, 148°49’,3–55’,4 E), central part of Sea of Okhotsk,
|
||
<quantity id="49739B56FFBA6845E4ED4E6209D5EA51" box="[151,228,512,538]" metricMagnitude="2" metricUnit="m" metricValue="5.0" pageId="5" pageNumber="62" unit="m" value="500.0">500 m</quantity>
|
||
,
|
||
<date id="FA351073FFBA6845E4954E6208B5EA51" box="[239,388,512,538]" pageId="5" pageNumber="62" value="1984-10-20">20 Oct.1984</date>
|
||
. Collector is A.V. Ereskovsky.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<caption id="DAF4663BFFBA6845E4ED4BC90D2CEFAE" httpUri="https://zenodo.org/record/175837/files/figure.png" pageId="5" pageNumber="62" targetBox="[333,1267,594,1947]" targetPageId="5">
|
||
<paragraph id="8E3436B3FFBA6845E4ED4BC90D2CEFAE" blockId="5.[151,1436,1963,2021]" pageId="5" pageNumber="62">
|
||
<emphasis id="BCFFEAA1FFBA6845E4ED4BC90828EF88" bold="true" box="[151,281,1963,1987]" pageId="5" pageNumber="62">FIGURE 2.</emphasis>
|
||
External morphology of
|
||
<taxonomicName id="498B4D30FFBA6845E6504BC90A20EF89" box="[554,785,1963,1986]" class="Demospongiae" family="Halisarcidae" genus="Halisarca" kingdom="Animalia" order="Chondrosida" pageId="5" pageNumber="62" phylum="Porifera" rank="species" species="korotkovae" status="sp. nov.">
|
||
<emphasis id="BCFFEAA1FFBA6845E6504BC90A20EF89" box="[554,785,1963,1986]" italics="true" pageId="5" pageNumber="62">Halisarca korotkovae</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="BCFFEAA1FFBA6845E76D4BC90A5CEF88" bold="true" box="[791,877,1963,1987]" pageId="5" pageNumber="62">
|
||
<taxonomicNameLabel id="A7CC57DAFFBA6845E76D4BC90A5CEF88" box="[791,877,1963,1987]" pageId="5" pageNumber="62" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
A) Photograph of a specimen with even surface. B) Photograph of a type specimen (ZIN RAS 10700) with a slightly undulating surface.
|
||
</paragraph>
|
||
</caption>
|
||
<caption id="DAF4663BFFB96846E4ED4AB30DC6EF1B" httpUri="https://zenodo.org/record/175838/files/figure.png" pageId="6" pageNumber="63" targetBox="[264,1335,194,1731]" targetPageId="6">
|
||
<paragraph id="8E3436B3FFB96846E4ED4AB30DC6EF1B" blockId="6.[151,1436,1745,1872]" pageId="6" pageNumber="63">
|
||
<emphasis id="BCFFEAA1FFB96846E4ED4AB3083FEEA2" bold="true" box="[151,270,1745,1769]" pageId="6" pageNumber="63">FIGURE 3</emphasis>
|
||
. The anatomical structures of
|
||
<taxonomicName id="498B4D30FFB96846E63C4AB30A12EEA3" box="[582,803,1745,1768]" class="Demospongiae" family="Halisarcidae" genus="Halisarca" kingdom="Animalia" order="Chondrosida" pageId="6" pageNumber="63" phylum="Porifera" rank="species" species="korotkovae" status="sp. nov.">
|
||
<emphasis id="BCFFEAA1FFB96846E63C4AB30A12EEA3" box="[582,803,1745,1768]" italics="true" pageId="6" pageNumber="63">Halisarca korotkovae</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="BCFFEAA1FFB96846E7504AB30A4DEEA2" bold="true" box="[810,892,1745,1769]" pageId="6" pageNumber="63">
|
||
<taxonomicNameLabel id="A7CC57DAFFB96846E7504AB30A4DEEA2" box="[810,892,1745,1769]" pageId="6" pageNumber="63" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
A) Light microscopy micrograph showing the external part of sponge with the ectosome. Stained with Mayer's hematoxylin-eosin. B) Light microscopy micrograph showing the internal part of sponge with the choanosome. Stained with Heidenhein’s hematoxylin-eosin. Abbreviation: C — cuticle; Cc — choanocyte chamber; Exc — exhalant canal; Mc — microgranular cells; Se — subectosomal cavities.
|
||
</paragraph>
|
||
</caption>
|
||
<subSubSection id="C6916538FFB96848E4BC4B1C0B46EC69" lastPageId="8" lastPageNumber="65" pageId="6" pageNumber="63" type="description">
|
||
<paragraph id="8E3436B3FFB96847E4BC4B1C0803E891" blockId="6.[151,1436,1918,2025]" lastBlockId="7.[151,1437,152,778]" lastPageId="7" lastPageNumber="64" pageId="6" pageNumber="63">
|
||
<emphasis id="BCFFEAA1FFB96846E4BC4B1C086EEFD3" bold="true" box="[198,351,1918,1944]" pageId="6" pageNumber="63">Description.</emphasis>
|
||
The sponge is encrusting,
|
||
<quantity id="49739B56FFB96846E6CB4B1D0A29EFD2" box="[689,792,1919,1945]" metricMagnitude="-3" metricUnit="m" metricValue="2.0" metricValueMax="3.0" metricValueMin="1.0" pageId="6" pageNumber="63" unit="mm" value="2.0" valueMax="3.0" valueMin="1.0">1–3 mm</quantity>
|
||
thick, spreading on a skeleton of
|
||
<taxonomicName id="498B4D30FFB96846E0BC4B1D0C5FEFD3" box="[1222,1390,1919,1944]" class="Hexactinellida" family="Aphrocallistidae" genus="Aphrocallistes" kingdom="Animalia" order="Hexactinosida" pageId="6" pageNumber="63" phylum="Porifera" rank="genus">
|
||
<emphasis id="BCFFEAA1FFB96846E0BC4B1D0C5FEFD3" box="[1222,1390,1919,1944]" italics="true" pageId="6" pageNumber="63">Aphrocallistes</emphasis>
|
||
</taxonomicName>
|
||
sp. (Hexactinellida) and forming patches from
|
||
<quantity id="49739B56FFB96846E6E04BC50BE7EF8A" box="[666,726,1959,1985]" metricMagnitude="-2" metricUnit="m" metricValue="1.0" pageId="6" pageNumber="63" unit="cm" value="1.0">1 cm</quantity>
|
||
up to
|
||
<quantity id="49739B56FFB96846E75E4BC50A6EEF8A" box="[804,863,1959,1985]" metricMagnitude="-2" metricUnit="m" metricValue="5.0" pageId="6" pageNumber="63" unit="cm" value="5.0">5 cm</quantity>
|
||
in diameter. Surface is skin-like, smooth, shiny, even or bearing a slight undulation (
|
||
<figureCitation id="16B02A36FFB96846E6474BAD0BB5EFA2" box="[573,644,1999,2025]" captionStart="FIGURE 2" captionStartId="5.[151,255,1963,1987]" captionTargetBox="[333,1267,594,1947]" captionTargetId="figure@5.[333,1267,594,1947]" captionTargetPageId="5" captionText="FIGURE 2. External morphology of Halisarca korotkovae sp. nov. A) Photograph of a specimen with even surface. B) Photograph of a type specimen (ZIN RAS 10700) with a slightly undulating surface." httpUri="https://zenodo.org/record/175837/files/figure.png" pageId="6" pageNumber="63">Fig. 2</figureCitation>
|
||
). Texture is softly collagenous, easily torn. No oscules or pores visible in preserved specimens. Colour in life is dull brownish-yellow, pigmentation is uniform throughout the sponge body.
|
||
</paragraph>
|
||
<paragraph id="8E3436B3FFB86847E4BC4C850BCBE981" blockId="7.[151,1437,152,778]" pageId="7" pageNumber="64">
|
||
<emphasis id="BCFFEAA1FFB86847E4BC4C8508C0E94A" bold="true" box="[198,497,231,257]" pageId="7" pageNumber="64">Soft tissue organisation.</emphasis>
|
||
A thin ectosomal region 17 to 55 µm deep is bounded by a cuticle from 2.9 to 5 µm deep (
|
||
<figureCitation id="16B02A36FFB86847E5764D720861E961" box="[268,336,272,298]" captionStart="FIGURE 3" captionStartId="6.[151,251,1745,1769]" captionTargetBox="[264,1335,194,1731]" captionTargetId="figure@6.[264,1335,194,1731]" captionTargetPageId="6" captionText="FIGURE 3. The anatomical structures of Halisarca korotkovae sp. nov. A) Light microscopy micrograph showing the external part of sponge with the ectosome. Stained with Mayer's hematoxylin-eosin. B) Light microscopy micrograph showing the internal part of sponge with the choanosome. Stained with Heidenhein’s hematoxylin-eosin. Abbreviation: C — cuticle; Cc — choanocyte chamber; Exc — exhalant canal; Mc — microgranular cells; Se — subectosomal cavities." httpUri="https://zenodo.org/record/175838/files/figure.png" pageId="7" pageNumber="64">Fig. 3</figureCitation>
|
||
A). The cuticle is mucus-like and acellular–the product of ectosome cell secretion overlapping all of the sponge surface (
|
||
<figureCitation id="16B02A36FFB86847E5BD4D5A0B26E919" box="[455,535,312,338]" captionStart="FIGURE 3" captionStartId="6.[151,251,1745,1769]" captionTargetBox="[264,1335,194,1731]" captionTargetId="figure@6.[264,1335,194,1731]" captionTargetPageId="6" captionText="FIGURE 3. The anatomical structures of Halisarca korotkovae sp. nov. A) Light microscopy micrograph showing the external part of sponge with the ectosome. Stained with Mayer's hematoxylin-eosin. B) Light microscopy micrograph showing the internal part of sponge with the choanosome. Stained with Heidenhein’s hematoxylin-eosin. Abbreviation: C — cuticle; Cc — choanocyte chamber; Exc — exhalant canal; Mc — microgranular cells; Se — subectosomal cavities." httpUri="https://zenodo.org/record/175838/files/figure.png" pageId="7" pageNumber="64">Figs. 3</figureCitation>
|
||
A, 4A). Beneath this cuticle is a dense cellular layer in which the cell bodies of T-shaped exopinacocytes occur, as well as conglomerates of big microgranular cells, rare spherulous cells, and vacuolar cells. Below this layer, cell density is greatly reduced in the mesohyl between the abundant inhalant canals and subectosomal cavities (
|
||
<figureCitation id="16B02A36FFB86847E6F54DD20BE5E981" box="[655,724,432,458]" captionStart="FIGURE 3" captionStartId="6.[151,251,1745,1769]" captionTargetBox="[264,1335,194,1731]" captionTargetId="figure@6.[264,1335,194,1731]" captionTargetPageId="6" captionText="FIGURE 3. The anatomical structures of Halisarca korotkovae sp. nov. A) Light microscopy micrograph showing the external part of sponge with the ectosome. Stained with Mayer's hematoxylin-eosin. B) Light microscopy micrograph showing the internal part of sponge with the choanosome. Stained with Heidenhein’s hematoxylin-eosin. Abbreviation: C — cuticle; Cc — choanocyte chamber; Exc — exhalant canal; Mc — microgranular cells; Se — subectosomal cavities." httpUri="https://zenodo.org/record/175838/files/figure.png" pageId="7" pageNumber="64">Fig. 3</figureCitation>
|
||
A).
|
||
</paragraph>
|
||
<paragraph id="8E3436B3FFB86847E4BC4DBA0AA9EA09" blockId="7.[151,1437,152,778]" pageId="7" pageNumber="64">
|
||
The choanosome makes up the greatest volume of the sponge body and is composed of choanocyte chambers and mesohyl (
|
||
<figureCitation id="16B02A36FFB86847E5084E620886EA51" box="[370,439,512,538]" captionStart="FIGURE 3" captionStartId="6.[151,251,1745,1769]" captionTargetBox="[264,1335,194,1731]" captionTargetId="figure@6.[264,1335,194,1731]" captionTargetPageId="6" captionText="FIGURE 3. The anatomical structures of Halisarca korotkovae sp. nov. A) Light microscopy micrograph showing the external part of sponge with the ectosome. Stained with Mayer's hematoxylin-eosin. B) Light microscopy micrograph showing the internal part of sponge with the choanosome. Stained with Heidenhein’s hematoxylin-eosin. Abbreviation: C — cuticle; Cc — choanocyte chamber; Exc — exhalant canal; Mc — microgranular cells; Se — subectosomal cavities." httpUri="https://zenodo.org/record/175838/files/figure.png" pageId="7" pageNumber="64">Fig. 3</figureCitation>
|
||
B). The choanocyte chambers are meandering tubular, long, branching, and tight with diameter of 1.5 to 10 µm and length up to 120 µm (
|
||
<figureCitation id="16B02A36FFB86847E68B4E4A0A72EA09" box="[753,835,552,578]" captionStart="FIGURE 3" captionStartId="6.[151,251,1745,1769]" captionTargetBox="[264,1335,194,1731]" captionTargetId="figure@6.[264,1335,194,1731]" captionTargetPageId="6" captionText="FIGURE 3. The anatomical structures of Halisarca korotkovae sp. nov. A) Light microscopy micrograph showing the external part of sponge with the ectosome. Stained with Mayer's hematoxylin-eosin. B) Light microscopy micrograph showing the internal part of sponge with the choanosome. Stained with Heidenhein’s hematoxylin-eosin. Abbreviation: C — cuticle; Cc — choanocyte chamber; Exc — exhalant canal; Mc — microgranular cells; Se — subectosomal cavities." httpUri="https://zenodo.org/record/175838/files/figure.png" pageId="7" pageNumber="64">Figs. 3</figureCitation>
|
||
B, 4B).
|
||
</paragraph>
|
||
<paragraph id="8E3436B3FFB86847E4BC4E2D0BE7EAF1" blockId="7.[151,1437,152,778]" pageId="7" pageNumber="64">
|
||
<emphasis id="BCFFEAA1FFB86847E4BC4E2D0B36EA22" bold="true" box="[198,519,591,617]" pageId="7" pageNumber="64">Cytology. Exopinacocytes</emphasis>
|
||
(
|
||
<figureCitation id="16B02A36FFB86847E6614E320B55EA21" box="[539,612,592,618]" captionStart="FIGURE 4" captionStartId="7.[151,255,1804,1828]" captionTargetBox="[151,1435,834,1779]" captionTargetId="figure@7.[151,1436,834,1780]" captionTargetPageId="7" captionText="FIGURE 4. Light microscopy micrographs of the cells of Halisarca korotkovae sp. nov. A) Ectosome with cuticle (C), microgranular cells (Mc), exopinacocytes (Ex), amoebocyte (Ac), spherulous cells (Sc), and vacuolar cell (Vc). Stained with Mayer's hematoxylin-eosin. B – D) Choanosome. B) Choanocyte chambers (Cc) consist of choanocytes (Ch), endopinacocytes (En) and spherulous cells (Sc). Stained with Heidenhein’s hematoxylin-eosin. C) Choanosomal mesohyl with vacuolar cells (Vc) and choanocyte chamber (Cc). Stained with Mayer's hematoxylin-eosin. D) Oocytes (O), spherulous cells (Sc) and amoebocyte (Ac) in the choanosome. Stained with Heidenhein’s hematoxylin-eosin." httpUri="https://zenodo.org/record/175839/files/figure.png" pageId="7" pageNumber="64">Fig. 4</figureCitation>
|
||
A) are T-shaped, as typical for order, with thin external cytoplasmic part and internal cell body part. The cell body, about 3.4 µm in diameter, is oval or irregular in form. Both parts are connected by a thin cytoplasmic bridge.
|
||
</paragraph>
|
||
<paragraph id="8E3436B3FFB86847E4BC4EA50A60EB41" blockId="7.[151,1437,152,778]" pageId="7" pageNumber="64">
|
||
<emphasis id="BCFFEAA1FFB86847E4BC4EA508ABEAAA" bold="true" box="[198,410,711,737]" pageId="7" pageNumber="64">Endopinacocytes</emphasis>
|
||
(
|
||
<figureCitation id="16B02A36FFB86847E5D64EAA08C4EAA9" box="[428,501,712,738]" captionStart="FIGURE 4" captionStartId="7.[151,255,1804,1828]" captionTargetBox="[151,1435,834,1779]" captionTargetId="figure@7.[151,1436,834,1780]" captionTargetPageId="7" captionText="FIGURE 4. Light microscopy micrographs of the cells of Halisarca korotkovae sp. nov. A) Ectosome with cuticle (C), microgranular cells (Mc), exopinacocytes (Ex), amoebocyte (Ac), spherulous cells (Sc), and vacuolar cell (Vc). Stained with Mayer's hematoxylin-eosin. B – D) Choanosome. B) Choanocyte chambers (Cc) consist of choanocytes (Ch), endopinacocytes (En) and spherulous cells (Sc). Stained with Heidenhein’s hematoxylin-eosin. C) Choanosomal mesohyl with vacuolar cells (Vc) and choanocyte chamber (Cc). Stained with Mayer's hematoxylin-eosin. D) Oocytes (O), spherulous cells (Sc) and amoebocyte (Ac) in the choanosome. Stained with Heidenhein’s hematoxylin-eosin." httpUri="https://zenodo.org/record/175839/files/figure.png" pageId="7" pageNumber="64">Fig. 4</figureCitation>
|
||
B) are flat, nonflagellate cells with nucleolate nucleus. The length is about 14 µm and the thickness in the nucleus region is about 1.8 µm.
|
||
</paragraph>
|
||
<caption id="DAF4663BFFB86847E4ED4B6E0D81EF9A" httpUri="https://zenodo.org/record/175839/files/figure.png" pageId="7" pageNumber="64" targetBox="[151,1435,834,1779]" targetPageId="7">
|
||
<paragraph id="8E3436B3FFB86847E4ED4B6E0D81EF9A" blockId="7.[151,1437,1804,2001]" pageId="7" pageNumber="64">
|
||
<emphasis id="BCFFEAA1FFB86847E4ED4B6E0828EF6F" bold="true" box="[151,281,1804,1828]" pageId="7" pageNumber="64">FIGURE 4.</emphasis>
|
||
Light microscopy micrographs of the cells of
|
||
<taxonomicName id="498B4D30FFB86847E7744B6E0AC5EF68" box="[782,1012,1804,1827]" class="Demospongiae" family="Halisarcidae" genus="Halisarca" kingdom="Animalia" order="Chondrosida" pageId="7" pageNumber="64" phylum="Porifera" rank="species" species="korotkovae" status="sp. nov.">
|
||
<emphasis id="BCFFEAA1FFB86847E7744B6E0AC5EF68" box="[782,1012,1804,1827]" italics="true" pageId="7" pageNumber="64">Halisarca korotkovae</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="BCFFEAA1FFB86847E7864B6E0D60EF6F" bold="true" box="[1020,1105,1804,1828]" pageId="7" pageNumber="64">
|
||
<taxonomicNameLabel id="A7CC57DAFFB86847E7864B6E0D60EF6F" box="[1020,1105,1804,1828]" pageId="7" pageNumber="64" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
A) Ectosome with cuticle (C), microgranular cells (Mc), exopinacocytes (Ex), amoebocyte (Ac), spherulous cells (Sc), and vacuolar cell (Vc). Stained with Mayer's hematoxylin-eosin. B–D) Choanosome. B) Choanocyte chambers (Cc) consist of choanocytes (Ch), endopinacocytes (En) and spherulous cells (Sc). Stained with Heidenhein’s hematoxylin-eosin. C) Choanosomal mesohyl with vacuolar cells (Vc) and choanocyte chamber (Cc). Stained with Mayer's hematoxylin-eosin. D) Oocytes (O), spherulous cells (Sc) and amoebocyte (Ac) in the choanosome. Stained with Heidenhein’s hematoxylin-eosin.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8E3436B3FFB76848E4BC4CF50C6CE8F9" blockId="8.[151,1437,151,1138]" box="[198,1373,151,178]" pageId="8" pageNumber="65">
|
||
<emphasis id="BCFFEAA1FFB76848E4BC4CF50857E8FA" bold="true" box="[198,358,151,177]" pageId="8" pageNumber="65">Choanocytes</emphasis>
|
||
are small, elongated, monoflagellate cells about 3.3×2.1 µm with anucleolate nucleus.
|
||
</paragraph>
|
||
<paragraph id="8E3436B3FFB76848E4BC4CDD08F7E931" blockId="8.[151,1437,151,1138]" pageId="8" pageNumber="65">
|
||
<emphasis id="BCFFEAA1FFB76848E4BC4CDD08DEE892" bold="true" box="[198,495,191,217]" pageId="8" pageNumber="65">Big microgranular cells</emphasis>
|
||
(
|
||
<figureCitation id="16B02A36FFB76848E67A4CA20B63E891" box="[512,594,192,218]" captionStart="FIGURE 3" captionStartId="6.[151,251,1745,1769]" captionTargetBox="[264,1335,194,1731]" captionTargetId="figure@6.[264,1335,194,1731]" captionTargetPageId="6" captionText="FIGURE 3. The anatomical structures of Halisarca korotkovae sp. nov. A) Light microscopy micrograph showing the external part of sponge with the ectosome. Stained with Mayer's hematoxylin-eosin. B) Light microscopy micrograph showing the internal part of sponge with the choanosome. Stained with Heidenhein’s hematoxylin-eosin. Abbreviation: C — cuticle; Cc — choanocyte chamber; Exc — exhalant canal; Mc — microgranular cells; Se — subectosomal cavities." httpUri="https://zenodo.org/record/175838/files/figure.png" pageId="8" pageNumber="65">Figs. 3</figureCitation>
|
||
A, 4A) are distinctive cells of the new species. These cells are concentrated mainly in the ectosome of the sponge. They are large, oval or egg-shaped (18.7×9.5 to 10.8×7.1 µm) with nucleolate nucleus (nucleolus diameter 1 µm). The cytoplasm is eosinophilic and filled with numerous small (0.5 µm in diameter) homogenous granules. These cells are often concentrated in aggregates up to 40 µm in diameter (
|
||
<figureCitation id="16B02A36FFB76848E5204D020890E931" box="[346,417,352,378]" captionStart="FIGURE 4" captionStartId="7.[151,255,1804,1828]" captionTargetBox="[151,1435,834,1779]" captionTargetId="figure@7.[151,1436,834,1780]" captionTargetPageId="7" captionText="FIGURE 4. Light microscopy micrographs of the cells of Halisarca korotkovae sp. nov. A) Ectosome with cuticle (C), microgranular cells (Mc), exopinacocytes (Ex), amoebocyte (Ac), spherulous cells (Sc), and vacuolar cell (Vc). Stained with Mayer's hematoxylin-eosin. B – D) Choanosome. B) Choanocyte chambers (Cc) consist of choanocytes (Ch), endopinacocytes (En) and spherulous cells (Sc). Stained with Heidenhein’s hematoxylin-eosin. C) Choanosomal mesohyl with vacuolar cells (Vc) and choanocyte chamber (Cc). Stained with Mayer's hematoxylin-eosin. D) Oocytes (O), spherulous cells (Sc) and amoebocyte (Ac) in the choanosome. Stained with Heidenhein’s hematoxylin-eosin." httpUri="https://zenodo.org/record/175839/files/figure.png" pageId="8" pageNumber="65">Fig. 4</figureCitation>
|
||
A).
|
||
</paragraph>
|
||
<paragraph id="8E3436B3FFB76848E4BC4DE5083BEA51" blockId="8.[151,1437,151,1138]" pageId="8" pageNumber="65">
|
||
<emphasis id="BCFFEAA1FFB76848E4BC4DE50805E9EA" bold="true" box="[198,308,391,417]" pageId="8" pageNumber="65">Vacuolar</emphasis>
|
||
cells (
|
||
<figureCitation id="16B02A36FFB76848E5FD4DEA08FFE9E9" box="[391,462,392,418]" captionStart="FIGURE 4" captionStartId="7.[151,255,1804,1828]" captionTargetBox="[151,1435,834,1779]" captionTargetId="figure@7.[151,1436,834,1780]" captionTargetPageId="7" captionText="FIGURE 4. Light microscopy micrographs of the cells of Halisarca korotkovae sp. nov. A) Ectosome with cuticle (C), microgranular cells (Mc), exopinacocytes (Ex), amoebocyte (Ac), spherulous cells (Sc), and vacuolar cell (Vc). Stained with Mayer's hematoxylin-eosin. B – D) Choanosome. B) Choanocyte chambers (Cc) consist of choanocytes (Ch), endopinacocytes (En) and spherulous cells (Sc). Stained with Heidenhein’s hematoxylin-eosin. C) Choanosomal mesohyl with vacuolar cells (Vc) and choanocyte chamber (Cc). Stained with Mayer's hematoxylin-eosin. D) Oocytes (O), spherulous cells (Sc) and amoebocyte (Ac) in the choanosome. Stained with Heidenhein’s hematoxylin-eosin." httpUri="https://zenodo.org/record/175839/files/figure.png" pageId="8" pageNumber="65">Fig. 4</figureCitation>
|
||
A, C) are oval or irregular (approximately 7.6 µm in diameter) eosinophyllic cells with anucleolate nucleus. The cytoplasm contains big semi-transparent vacuoles (0.2–0.7 µm in diameter) often with microgranular inclusions. These cells are dispersed in the ectosome (
|
||
<figureCitation id="16B02A36FFB76848E0184DBA0D9AE9B9" box="[1122,1195,472,498]" captionStart="FIGURE 4" captionStartId="7.[151,255,1804,1828]" captionTargetBox="[151,1435,834,1779]" captionTargetId="figure@7.[151,1436,834,1780]" captionTargetPageId="7" captionText="FIGURE 4. Light microscopy micrographs of the cells of Halisarca korotkovae sp. nov. A) Ectosome with cuticle (C), microgranular cells (Mc), exopinacocytes (Ex), amoebocyte (Ac), spherulous cells (Sc), and vacuolar cell (Vc). Stained with Mayer's hematoxylin-eosin. B – D) Choanosome. B) Choanocyte chambers (Cc) consist of choanocytes (Ch), endopinacocytes (En) and spherulous cells (Sc). Stained with Heidenhein’s hematoxylin-eosin. C) Choanosomal mesohyl with vacuolar cells (Vc) and choanocyte chamber (Cc). Stained with Mayer's hematoxylin-eosin. D) Oocytes (O), spherulous cells (Sc) and amoebocyte (Ac) in the choanosome. Stained with Heidenhein’s hematoxylin-eosin." httpUri="https://zenodo.org/record/175839/files/figure.png" pageId="8" pageNumber="65">Fig. 4</figureCitation>
|
||
A) and choanosome (
|
||
<figureCitation id="16B02A36FFB76848E4E54E6209D7EA51" box="[159,230,512,538]" captionStart="FIGURE 4" captionStartId="7.[151,255,1804,1828]" captionTargetBox="[151,1435,834,1779]" captionTargetId="figure@7.[151,1436,834,1780]" captionTargetPageId="7" captionText="FIGURE 4. Light microscopy micrographs of the cells of Halisarca korotkovae sp. nov. A) Ectosome with cuticle (C), microgranular cells (Mc), exopinacocytes (Ex), amoebocyte (Ac), spherulous cells (Sc), and vacuolar cell (Vc). Stained with Mayer's hematoxylin-eosin. B – D) Choanosome. B) Choanocyte chambers (Cc) consist of choanocytes (Ch), endopinacocytes (En) and spherulous cells (Sc). Stained with Heidenhein’s hematoxylin-eosin. C) Choanosomal mesohyl with vacuolar cells (Vc) and choanocyte chamber (Cc). Stained with Mayer's hematoxylin-eosin. D) Oocytes (O), spherulous cells (Sc) and amoebocyte (Ac) in the choanosome. Stained with Heidenhein’s hematoxylin-eosin." httpUri="https://zenodo.org/record/175839/files/figure.png" pageId="8" pageNumber="65">Fig. 4</figureCitation>
|
||
C).
|
||
</paragraph>
|
||
<paragraph id="8E3436B3FFB76848E4BC4E450A40EAD9" blockId="8.[151,1437,151,1138]" pageId="8" pageNumber="65">
|
||
<emphasis id="BCFFEAA1FFB76848E4BC4E450865EA0A" bold="true" box="[198,340,551,577]" pageId="8" pageNumber="65">Spherulous</emphasis>
|
||
cells (
|
||
<figureCitation id="16B02A36FFB76848E5D84E4A08D8EA09" box="[418,489,552,578]" captionStart="FIGURE 4" captionStartId="7.[151,255,1804,1828]" captionTargetBox="[151,1435,834,1779]" captionTargetId="figure@7.[151,1436,834,1780]" captionTargetPageId="7" captionText="FIGURE 4. Light microscopy micrographs of the cells of Halisarca korotkovae sp. nov. A) Ectosome with cuticle (C), microgranular cells (Mc), exopinacocytes (Ex), amoebocyte (Ac), spherulous cells (Sc), and vacuolar cell (Vc). Stained with Mayer's hematoxylin-eosin. B – D) Choanosome. B) Choanocyte chambers (Cc) consist of choanocytes (Ch), endopinacocytes (En) and spherulous cells (Sc). Stained with Heidenhein’s hematoxylin-eosin. C) Choanosomal mesohyl with vacuolar cells (Vc) and choanocyte chamber (Cc). Stained with Mayer's hematoxylin-eosin. D) Oocytes (O), spherulous cells (Sc) and amoebocyte (Ac) in the choanosome. Stained with Heidenhein’s hematoxylin-eosin." httpUri="https://zenodo.org/record/175839/files/figure.png" pageId="8" pageNumber="65">Fig. 4</figureCitation>
|
||
A, B, D) are oval (6.2 - 7.9 µm in diameter) with anucleolate nucleus. The cytoplasm is filled with spherical granules (diameter from 0.8 to 2.9 µm) homogenous in content. These cells are dispersed in the ectosome (
|
||
<figureCitation id="16B02A36FFB76848E5AE4E1A0B2BEAD9" box="[468,538,632,658]" captionStart="FIGURE 4" captionStartId="7.[151,255,1804,1828]" captionTargetBox="[151,1435,834,1779]" captionTargetId="figure@7.[151,1436,834,1780]" captionTargetPageId="7" captionText="FIGURE 4. Light microscopy micrographs of the cells of Halisarca korotkovae sp. nov. A) Ectosome with cuticle (C), microgranular cells (Mc), exopinacocytes (Ex), amoebocyte (Ac), spherulous cells (Sc), and vacuolar cell (Vc). Stained with Mayer's hematoxylin-eosin. B – D) Choanosome. B) Choanocyte chambers (Cc) consist of choanocytes (Ch), endopinacocytes (En) and spherulous cells (Sc). Stained with Heidenhein’s hematoxylin-eosin. C) Choanosomal mesohyl with vacuolar cells (Vc) and choanocyte chamber (Cc). Stained with Mayer's hematoxylin-eosin. D) Oocytes (O), spherulous cells (Sc) and amoebocyte (Ac) in the choanosome. Stained with Heidenhein’s hematoxylin-eosin." httpUri="https://zenodo.org/record/175839/files/figure.png" pageId="8" pageNumber="65">Fig. 4</figureCitation>
|
||
A) choanosome (
|
||
<figureCitation id="16B02A36FFB76848E6994E1A0A18EAD9" box="[739,809,632,658]" captionStart="FIGURE 4" captionStartId="7.[151,255,1804,1828]" captionTargetBox="[151,1435,834,1779]" captionTargetId="figure@7.[151,1436,834,1780]" captionTargetPageId="7" captionText="FIGURE 4. Light microscopy micrographs of the cells of Halisarca korotkovae sp. nov. A) Ectosome with cuticle (C), microgranular cells (Mc), exopinacocytes (Ex), amoebocyte (Ac), spherulous cells (Sc), and vacuolar cell (Vc). Stained with Mayer's hematoxylin-eosin. B – D) Choanosome. B) Choanocyte chambers (Cc) consist of choanocytes (Ch), endopinacocytes (En) and spherulous cells (Sc). Stained with Heidenhein’s hematoxylin-eosin. C) Choanosomal mesohyl with vacuolar cells (Vc) and choanocyte chamber (Cc). Stained with Mayer's hematoxylin-eosin. D) Oocytes (O), spherulous cells (Sc) and amoebocyte (Ac) in the choanosome. Stained with Heidenhein’s hematoxylin-eosin." httpUri="https://zenodo.org/record/175839/files/figure.png" pageId="8" pageNumber="65">Fig. 4</figureCitation>
|
||
B, D).
|
||
</paragraph>
|
||
<paragraph id="8E3436B3FFB76848E4BC4EFD0AE4EAA9" blockId="8.[151,1437,151,1138]" pageId="8" pageNumber="65">
|
||
<emphasis id="BCFFEAA1FFB76848E4BC4EFD085DEAF2" bold="true" box="[198,364,671,697]" pageId="8" pageNumber="65">Amoebocytes</emphasis>
|
||
(
|
||
<figureCitation id="16B02A36FFB76848E5064EC208F0EAF1" box="[380,449,672,698]" captionStart="FIGURE 4" captionStartId="7.[151,255,1804,1828]" captionTargetBox="[151,1435,834,1779]" captionTargetId="figure@7.[151,1436,834,1780]" captionTargetPageId="7" captionText="FIGURE 4. Light microscopy micrographs of the cells of Halisarca korotkovae sp. nov. A) Ectosome with cuticle (C), microgranular cells (Mc), exopinacocytes (Ex), amoebocyte (Ac), spherulous cells (Sc), and vacuolar cell (Vc). Stained with Mayer's hematoxylin-eosin. B – D) Choanosome. B) Choanocyte chambers (Cc) consist of choanocytes (Ch), endopinacocytes (En) and spherulous cells (Sc). Stained with Heidenhein’s hematoxylin-eosin. C) Choanosomal mesohyl with vacuolar cells (Vc) and choanocyte chamber (Cc). Stained with Mayer's hematoxylin-eosin. D) Oocytes (O), spherulous cells (Sc) and amoebocyte (Ac) in the choanosome. Stained with Heidenhein’s hematoxylin-eosin." httpUri="https://zenodo.org/record/175839/files/figure.png" pageId="8" pageNumber="65">Fig. 4</figureCitation>
|
||
A, D) are ameboid cells (from 3.3 to 3.8 µm in diameter) with big nucleolate nucleus (2.1–2.4 µm in diameter), dispersed in all parts of the sponge mesohyl.
|
||
</paragraph>
|
||
<paragraph id="8E3436B3FFB76848E4BC4E8D0A7AEBC9" blockId="8.[151,1437,151,1138]" pageId="8" pageNumber="65">
|
||
<emphasis id="BCFFEAA1FFB76848E4BC4E8D084BEB42" bold="true" box="[198,378,751,777]" pageId="8" pageNumber="65">Reproduction.</emphasis>
|
||
In all investigated specimens there were many oocytes (
|
||
<figureCitation id="16B02A36FFB76848E04D4E920DB0EB41" box="[1079,1153,752,778]" captionStart="FIGURE 4" captionStartId="7.[151,255,1804,1828]" captionTargetBox="[151,1435,834,1779]" captionTargetId="figure@7.[151,1436,834,1780]" captionTargetPageId="7" captionText="FIGURE 4. Light microscopy micrographs of the cells of Halisarca korotkovae sp. nov. A) Ectosome with cuticle (C), microgranular cells (Mc), exopinacocytes (Ex), amoebocyte (Ac), spherulous cells (Sc), and vacuolar cell (Vc). Stained with Mayer's hematoxylin-eosin. B – D) Choanosome. B) Choanocyte chambers (Cc) consist of choanocytes (Ch), endopinacocytes (En) and spherulous cells (Sc). Stained with Heidenhein’s hematoxylin-eosin. C) Choanosomal mesohyl with vacuolar cells (Vc) and choanocyte chamber (Cc). Stained with Mayer's hematoxylin-eosin. D) Oocytes (O), spherulous cells (Sc) and amoebocyte (Ac) in the choanosome. Stained with Heidenhein’s hematoxylin-eosin." httpUri="https://zenodo.org/record/175839/files/figure.png" pageId="8" pageNumber="65">Fig. 4</figureCitation>
|
||
D) in an early stage of oogenesis before vitellogenesis. Oocytes have oval (from 9.2 to 14.5 µm in diameter) or elongated form (from 14.5×8.3 µm to 22.9×7.9 µm) and are dispersed in the mesohyl of the choanosome. The nucleus (about 5.2 µm in diameter) contains a nucleolus (2.9 µm in diameter).
|
||
</paragraph>
|
||
<paragraph id="8E3436B3FFB76848E4BC4FED0B46EC69" blockId="8.[151,1437,151,1138]" pageId="8" pageNumber="65">
|
||
<emphasis id="BCFFEAA1FFB76848E4BC4FED0B31EBE2" bold="true" box="[198,512,911,937]" pageId="8" pageNumber="65">Habitat and distribution.</emphasis>
|
||
Specimens of
|
||
<taxonomicName id="498B4D30FFB76848E6D44FF20A9AEBE2" box="[686,939,912,937]" class="Demospongiae" family="Halisarcidae" genus="Halisarca" kingdom="Animalia" order="Chondrosida" pageId="8" pageNumber="65" phylum="Porifera" rank="species" species="korotkovae" status="sp. nov.">
|
||
<emphasis id="BCFFEAA1FFB76848E6D44FF20A9AEBE2" box="[686,939,912,937]" italics="true" pageId="8" pageNumber="65">Halisarca korotkovae</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="BCFFEAA1FFB76848E7C94FED0D20EBE2" bold="true" box="[947,1041,911,937]" pageId="8" pageNumber="65">
|
||
<taxonomicNameLabel id="A7CC57DAFFB76848E7C94FED0D20EBE2" box="[947,1041,911,937]" pageId="8" pageNumber="65" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
occur as thin sheets on the skeleton of a hexactinellid sponge
|
||
<taxonomicName id="498B4D30FFB76848E58B4FDA0BA8EB9A" box="[497,665,952,977]" class="Hexactinellida" family="Aphrocallistidae" genus="Aphrocallistes" kingdom="Animalia" order="Hexactinosida" pageId="8" pageNumber="65" phylum="Porifera" rank="genus">
|
||
<emphasis id="BCFFEAA1FFB76848E58B4FDA0BA8EB9A" box="[497,665,952,977]" italics="true" pageId="8" pageNumber="65">Aphrocallistes</emphasis>
|
||
</taxonomicName>
|
||
sp. (
|
||
<taxonomicName id="498B4D30FFB76848E6A84FDA0A4FEB99" box="[722,894,952,978]" class="Hexactinellida" kingdom="Animalia" order="Hexactinosida" pageId="8" pageNumber="65" phylum="Porifera" rank="order">Hexactinosida</taxonomicName>
|
||
,
|
||
<taxonomicName id="498B4D30FFB76848E7F04FDA0D7FEB99" box="[906,1102,952,978]" class="Hexactinellida" family="Aphrocallistidae" kingdom="Animalia" order="Hexactinosida" pageId="8" pageNumber="65" phylum="Porifera" rank="family">Aphrocallistidae</taxonomicName>
|
||
) in the SW and central parts of Sea of Okhotsk at a depth from
|
||
<quantity id="49739B56FFB76848E6534F820BF5EBB1" box="[553,708,992,1018]" metricMagnitude="2" metricUnit="m" metricValue="4.725" metricValueMax="6.45" metricValueMin="3.0" pageId="8" pageNumber="65" unit="m" value="472.5" valueMax="645.0" valueMin="300.0">300 to 645 m</quantity>
|
||
(
|
||
<figureCitation id="16B02A36FFB76848E6AE4F820A28EBB1" box="[724,793,992,1018]" captionStart="FIGURE 1" captionStartId="1.[151,255,1874,1898]" captionTargetBox="[243,1343,197,1847]" captionTargetId="figure@1.[243,1345,197,1848]" captionTargetPageId="1" captionText="FIGURE 1. Location of the collection sites of Halisarca korotkovae sp. nov. in the Sea of Okhotsk (Pacific Ocean). The circles indicate the exact collecting sites. Arrow indicates the type locality." httpUri="https://zenodo.org/record/175836/files/figure.png" pageId="8" pageNumber="65">Fig. 1</figureCitation>
|
||
). The bottom consists of silt, pebbles and fragments of hexactinellid
|
||
<taxonomicName id="498B4D30FFB76848E54F486A08ECEC6A" box="[309,477,1032,1057]" class="Hexactinellida" family="Aphrocallistidae" genus="Aphrocallistes" kingdom="Animalia" order="Hexactinosida" pageId="8" pageNumber="65" phylum="Porifera" rank="genus">
|
||
<emphasis id="BCFFEAA1FFB76848E54F486A08ECEC6A" box="[309,477,1032,1057]" italics="true" pageId="8" pageNumber="65">Aphrocallistes</emphasis>
|
||
</taxonomicName>
|
||
sp. skeleton.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C6916538FFB76848E4BC484D0B0BEC39" pageId="8" pageNumber="65" type="etymology">
|
||
<paragraph id="8E3436B3FFB76848E4BC484D0B0BEC39" blockId="8.[151,1437,151,1138]" pageId="8" pageNumber="65">
|
||
<emphasis id="BCFFEAA1FFB76848E4BC484D0862EC02" bold="true" box="[198,339,1071,1097]" pageId="8" pageNumber="65">Etymology.</emphasis>
|
||
The species name refers to the famous Russian spongologist, Prof. Galina P. Korotkova (Saint-Petersburg State University).
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |