treatments-xml/data/03/F8/B1/03F8B169DB1DFFBA8FF5F89BFB6CFA6C.xml

<document id="1A9FBEF4F6B0346350CA210097FE301D" ID-DOI="10.5281/zenodo.5357614" ID-ISSN="2345-7600" ID-Zenodo-Dep="5357614" ID-ZooBank="069160EB-BA10-414D-87B4-0CC7DE25E53C" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1630220582872" checkinUser="valdenar" docAuthor="Mapalo, Marc A., Stec, Daniel, Mirano-Bascos, Denise &amp; Michalczyk, Łukasz" docDate="2017" docId="03F8B169DB1DFFBA8FF5F89BFB6CFA6C" docLanguage="en" docName="65rbz440-454.pdf" docOrigin="Raffles Bulletin of Zoology 65" docStyle="DocumentStyle:F2D34FF25DBF9998BB8DF6F24A5B097A.5:RafflesBZool.2014-2018.journal_article" docStyleId="F2D34FF25DBF9998BB8DF6F24A5B097A" docStyleName="RafflesBZool.2014-2018.journal_article" docStyleVersion="5" docTitle="Mesobiotus insanis Mapalo &amp; Stec &amp; Mirano-Bascos &amp; Michalczyk 2017, new species" docType="treatment" docVersion="3" lastPageNumber="445" masterDocId="FFC1C911DB1FFFBF8F0AFFA3FFF5FFD9" masterDocTitle="An integrative description of a limnoterrestrial tardigrade from the Philippines, Mesobiotus insanis, new species (Eutardigrada: Macrobiotidae: harmsworthi group)" masterLastPageNumber="454" masterPageNumber="440" pageNumber="442" updateTime="1699191141449" updateUser="plazi" zenodo-license-document="CC-BY-4.0">
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<mods:title id="B1D1D8E5D312C31E8B357E1B541256EE">An integrative description of a limnoterrestrial tardigrade from the Philippines, Mesobiotus insanis, new species (Eutardigrada: Macrobiotidae: harmsworthi group)</mods:title>
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<mods:namePart id="13F801D9BD7E81FC71DE46F1FF6573C6">Mapalo, Marc A.</mods:namePart>
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<mods:namePart id="C9282D994B77A68E638E1234DE5EA00F">Stec, Daniel</mods:namePart>
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<mods:namePart id="86513BB181B8326DE4B463F574CB3926">Mirano-Bascos, Denise</mods:namePart>
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<mods:namePart id="6E0A27C1584AEBC3BB7173C98E12E081">Michalczyk, Łukasz</mods:namePart>
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<mods:title id="1C09886D3388E28AF8AA68DAFCDDD30F">Raffles Bulletin of Zoology</mods:title>
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<mods:date id="3BFE5E04F07E477989FB260FC44900DE">2017</mods:date>
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<paragraph id="8BEE007FDB1DFFBD8FF5F89BFD90F889" blockId="2.[255,613,1848,1904]" box="[255,613,1848,1872]" pageId="2" pageNumber="442">
<heading id="D0A6B713DB1DFFBD8FF5F89BFD90F889" bold="true" box="[255,613,1848,1872]" centered="true" fontSize="10" level="2" pageId="2" pageNumber="442" reason="2">
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<taxonomicName id="4C517BFCDB1DFFBD8FF5F89BFE20F889" ID-CoL="8f3dd6a2-20f5-4095-be84-88012b29b718" authority="Mapalo &amp; Stec &amp; Mirano-Bascos &amp; Michalczyk, 2017" authorityName="Mapalo &amp; Stec &amp; Mirano-Bascos &amp; Michalczyk" authorityYear="2017" box="[255,469,1848,1872]" class="Eutardigrada" family="Macrobiotidae" genus="Mesobiotus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Parachela" pageId="2" pageNumber="442" phylum="Tardigrada" rank="species" species="insanis" status="sp. nov.">
<emphasis id="B925DC6DDB1DFFBD8FF5F89BFE20F889" bold="true" box="[255,469,1848,1872]" italics="true" pageId="2" pageNumber="442">Mesobiotus insanis</emphasis>
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<taxonomicNameLabel id="A2166116DB1DFFBD8ED5F89BFD90F889" box="[479,613,1848,1872]" pageId="2" pageNumber="442" rank="species">new species</taxonomicNameLabel>
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<paragraph id="8BEE007FDB1DFFBD8E7FF8FBFE19F8A9" blockId="2.[255,613,1848,1904]" box="[373,492,1880,1904]" pageId="2" pageNumber="442">
(
<figureCitation id="136A1CFADB1DFFBD8E77F8FBFE16F8A9" box="[381,483,1880,1904]" captionStart-0="Fig" captionStart-1="Fig" captionStart-2="Fig" captionStart-3="Fig" captionStart-4="Fig" captionStart-5="Fig" captionStart-6="Fig" captionStart-7="Fig" captionStartId-0="2.[113,148,1170,1192]" captionStartId-1="3.[135,170,1958,1980]" captionStartId-2="4.[113,148,1067,1089]" captionStartId-3="6.[113,148,2093,2115]" captionStartId-4="7.[135,170,2064,2086]" captionStartId-5="9.[136,171,1673,1695]" captionStartId-6="10.[113,148,2036,2058]" captionStartId-7="11.[135,170,1987,2009]" captionTargetBox-0="[113,1451,189,1155]" captionTargetBox-1="[136,1474,213,1942]" captionTargetBox-2="[113,1451,668,1051]" captionTargetBox-3="[114,1451,189,2077]" captionTargetBox-4="[146,1462,189,2049]" captionTargetBox-5="[135,1474,664,1657]" captionTargetBox-6="[134,1430,189,2020]" captionTargetBox-7="[136,1474,237,1971]" captionTargetId-0="figure-311@2.[113,1451,189,1155]" captionTargetId-1="figure-8@3.[136,1474,213,1942]" captionTargetId-2="figure-130@4.[113,1451,668,1051]" captionTargetId-3="figure-10@6.[114,1451,189,2077]" captionTargetId-4="figure-7@7.[146,1462,189,2049]" captionTargetId-5="figure-359@9.[135,1474,664,1657]" captionTargetId-6="figure-11@10.[134,1430,189,2020]" captionTargetId-7="figure-72@11.[136,1474,237,1971]" captionTargetPageId-0="2" captionTargetPageId-1="3" captionTargetPageId-2="4" captionTargetPageId-3="6" captionTargetPageId-4="7" captionTargetPageId-5="9" captionTargetPageId-6="10" captionTargetPageId-7="11" captionText-0="Fig. 1. Mesobiotus insanis, new species, PCM images of habitus and cuticle. A, dorso-ventral projection (holotype, PCM); B, dorsal cuticle above leg II; C, ventral cuticle of legs IV. Empty arrowheads indicate granulation; the filled arrowhead indicates the horse-shoe shaped structure connecting the anterior and the posterior claw. Scale bars in μm." captionText-1="Fig. 2. Mesobiotus insanis, new species, PCM images of the buccal apparatus. A, an entire buccal apparatus (paratype); B, dorsal teeth; C–E, ventral teeth (in Fig C, D, E, the medio-ventral tooth is divided into three, four, and five oval teeth, respectively); F, ventral placoids; G, dorsal placoids. Filled flat arrowheads indicate the first band of teeth, empty flat arrowheads indicate the second band of teeth, filled indented arrowheads indicate the third band of teeth, empty arrows indicate the additional teeth in the third band, empty indented arrowheads indicate subterminal constrictions in the third macroplacoid. Scale bars in μm." captionText-2="Fig. 3. Mesobiotus insanis, new species, PCM images of claws. A, claws I with smooth lunules; B, claws II with smooth lunules; C, claws IV with slightly crenulated lunules. Arrows indicate the W-shaped cuticular bars, the filled arrowhead indicates the horseshoe-shaped structure connecting the anterior and the posterior claw, the empty arrowheads indicate leg granulation. Scale in μm." captionText-3="Fig. 4. Mesobiotus insanis, new species, PCM images of midsections of six different eggs. Scale bars in μm." captionText-4="Fig. 5. Mesobiotus insanis, new species, PCM images of the surfaces of the eggs shown in Fig. 4 (respective letters indicate same eggs in both figures). Scale bars in μm." captionText-5="Fig. 6. Mesobiotus insanis, new species, PCM images of the midsection of processes from various eggs. Scale bars in μm." captionText-6="Fig. 7. Mesobiotus insanis, new species, magnified PCM images of the surface of the eggs shown in Fig. 4 (respective letters indicate same eggs in both figures). Filled arrowheads indicate the whorled sculpturing inside areolae; empty arrowheads indicate the bubble-like structures within the rims delimiting the areolae. Scale bars in μm." captionText-7="Fig. 8. Mesobiotus insanis, new species, SEM images of eggs. A, B, entire view of two different eggs; C, D, egg surface with processes; E, egg process; F, egg surface between processes, with evident pores and whorl-shaped sculpturing. Arrows indicate pores in the processes, flat arrowheads indicate the whorl-shaped surface inside the areolae, indented arrowheads indicate pores inside the areolae. Scale bars in μm." figureDoi-0="http://doi.org/10.5281/zenodo.5357616" figureDoi-1="http://doi.org/10.5281/zenodo.5357618" figureDoi-2="http://doi.org/10.5281/zenodo.5357620" figureDoi-3="http://doi.org/10.5281/zenodo.5357622" figureDoi-4="http://doi.org/10.5281/zenodo.5357624" figureDoi-5="http://doi.org/10.5281/zenodo.5357626" figureDoi-6="http://doi.org/10.5281/zenodo.5357628" figureDoi-7="http://doi.org/10.5281/zenodo.5357630" httpUri-0="https://zenodo.org/record/5357616/files/figure.png" httpUri-1="https://zenodo.org/record/5357618/files/figure.png" httpUri-2="https://zenodo.org/record/5357620/files/figure.png" httpUri-3="https://zenodo.org/record/5357622/files/figure.png" httpUri-4="https://zenodo.org/record/5357624/files/figure.png" httpUri-5="https://zenodo.org/record/5357626/files/figure.png" httpUri-6="https://zenodo.org/record/5357628/files/figure.png" httpUri-7="https://zenodo.org/record/5357630/files/figure.png" pageId="2" pageNumber="442">Figs. 1–8</figureCitation>
)
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<emphasis id="B925DC6DDB1DFFBD8F7BF83BFEBEF869" bold="true" box="[113,331,1944,1968]" pageId="2" pageNumber="442">Material examined.</emphasis>
<materialsCitation id="3B390A22DB1DFFBD8E45F83BFC56FA08" collectingDate="2014-05" collectionCode="UP" collectorName="Coll. Marc Mapalo &amp; Mika Berza &amp; Cali Fernandez" country="Philippines" county="All" elevation="60" latitude="14.648944" location="College of Science Library" longLatPrecision="1" longitude="121.06953" municipality="Science Park" pageId="2" pageNumber="442" specimenCount="71" specimenCount-egg="35" specimenCount-female="7" stateProvince="Quezon" typeStatus="holotype">
<typeStatus id="54EABEDDDB1DFFBD8E45F83BFE44F869" box="[335,433,1944,1968]" pageId="2" pageNumber="442" type="holotype">Holotype</typeStatus>
(slide number: PH.003.12), 
<specimenCount id="9D57CBF6DB1DFFBD8DDCF83BFF22F808" pageId="2" pageNumber="442" type="generic" typeStatus="paratypes">29 paratypes</specimenCount>
(slide numbers: 
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.003.03–04, 06, 08–13, 15–16, 20), and 
<specimenCount id="9D57CBF6DB1DFFBD8FC7F87BFEEBF828" box="[205,286,2008,2033]" pageId="2" pageNumber="442" type="egg">29 eggs</specimenCount>
(slide numbers: PH.003.07, 22, 
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.004.01– 03; 
<specimenCount id="9D57CBF6DB1DFFBD8F90F85BFF2BF7C8" box="[154,222,2040,2065]" pageId="2" pageNumber="442" type="egg">6 eggs</specimenCount>
on SEM stubs) as well as 
<specimenCount id="9D57CBF6DB1DFFBD8EFFF85AFDA9F7C9" box="[501,604,2040,2064]" pageId="2" pageNumber="442" type="female">7 females</specimenCount>
processed for DNA sequencing. 
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examined individuals and eggs were derived from a single female isolated from moss on the trunk of a tree growing in the 
<collectionCode id="ED4098BADB1DFFBD8B3FFA9AFBA2FA89" box="[1077,1111,1337,1360]" country="Papua New Guinea" name="University of Papua and New Guinea" pageId="2" pageNumber="442">UP</collectionCode>
<collectingMunicipality id="6B8A9A05DB1DFFBD8B55FA9AFB1BFA88" box="[1119,1262,1337,1361]" pageId="2" pageNumber="442">Science Park</collectingMunicipality>
, near the 
<location id="8E8E56A4DB1DFFBD8A52FA9AFC0FFAA8" LSID="urn:lsid:plazi:treatment:03F8B169DB1DFFBA8FF5F89BFB6CFA6C:8E8E56A4DB1DFFBD8A52FA9AFC0FFAA8" country="Philippines" county="All" latitude="14.648944" longLatPrecision="1" longitude="121.06953" municipality="Science Park" name="College of Science Library" pageId="2" pageNumber="442" stateProvince="Quezon">College of Science Library</location>
, 
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of the Philippines, 
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, 
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, 
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(
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, 
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, 
<elevation id="007CE74CDB1DFFBD8A9BFADAFC9CFA68" metricMagnitude="1" metricUnit="m" metricValue="6.0" pageId="2" pageNumber="442" unit="m" value="60.0">
<quantity id="4CA9AD9ADB1DFFBD8A9BFADAFCB7FA69" metricMagnitude="1" metricUnit="m" metricValue="6.0" pageId="2" pageNumber="442" unit="m" value="60.0">60 m</quantity>
asl
</elevation>
). 
<collectorName id="26A465A9DB1DFFBD8C71FA3AFBBCFA68" box="[891,1097,1433,1457]" pageId="2" pageNumber="442">Coll. Marc Mapalo</collectorName>
, 
<collectorName id="26A465A9DB1DFFBD8B59FA3AFB25FA69" box="[1107,1232,1433,1457]" pageId="2" pageNumber="442">Mika Berza</collectorName>
and 
<collectorName id="26A465A9DB1DFFBD8A09FA3AFA5DFA68" box="[1283,1448,1433,1457]" pageId="2" pageNumber="442">Cali Fernandez</collectorName>
, 
<date id="FFEF26BFDB1DFFBD8C27FA1AFC6AFA08" box="[813,927,1465,1489]" pageId="2" pageNumber="442" value="2014-05">
<collectingDate id="EFABDF57DB1DFFBD8C27FA1AFC6AFA08" box="[813,927,1465,1489]" pageId="2" pageNumber="442" value="2014-05">May 2014</collectingDate>
</date>
.
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</paragraph>
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<subSubSection id="C34B53F4DB1DFFBA8C27FA5BFBFCFEAC" lastPageId="5" lastPageNumber="445" pageId="2" pageNumber="442" type="description">
<paragraph id="8BEE007FDB1DFFBD8C27FA5BFAD4F9A8" blockId="2.[813,1452,1528,1649]" pageId="2" pageNumber="442">
<emphasis id="B925DC6DDB1DFFBD8C27FA5BFB76F9C9" bold="true" box="[813,1155,1528,1553]" pageId="2" pageNumber="442">Description of the new species.</emphasis>
Animals (morphometrics in 
<tableCitation id="C6D335C4DB1DFFBD8C27F9BAFC77F9E8" box="[813,898,1561,1585]" captionStart="Table 4" captionStartId="8.[113,169,667,689]" captionText="Table 4. Measurements (in μm) of selected morphological structures of individuals of Mesobiotus insanis, new species, mounted in Hoyer’s medium (N—number of specimens/structures measured. Range refers to the smallest and the largest structure among all measured specimens; SD—standard deviation)." pageId="2" pageNumber="442">Table 4</tableCitation>
): Body white/transparent (
<figureCitation id="136A1CFADB1DFFBD8BA9F9BAFB0CF9E8" box="[1187,1273,1561,1585]" captionStart="Fig" captionStartId="2.[113,148,1170,1192]" captionTargetBox="[113,1451,189,1155]" captionTargetId="figure-311@2.[113,1451,189,1155]" captionTargetPageId="2" captionText="Fig. 1. Mesobiotus insanis, new species, PCM images of habitus and cuticle. A, dorso-ventral projection (holotype, PCM); B, dorsal cuticle above leg II; C, ventral cuticle of legs IV. Empty arrowheads indicate granulation; the filled arrowhead indicates the horse-shoe shaped structure connecting the anterior and the posterior claw. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357616" httpUri="https://zenodo.org/record/5357616/files/figure.png" pageId="2" pageNumber="442">Fig. 1A</figureCitation>
). Eyes absent in live individuals. Cuticle without pores. Granulation present on legs I–IV (
<figureCitation id="136A1CFADB1DFFBD8CCDF9FAFBC8F9A8" box="[967,1085,1625,1649]" captionStart="Fig" captionStartId="2.[113,148,1170,1192]" captionTargetBox="[113,1451,189,1155]" captionTargetId="figure-311@2.[113,1451,189,1155]" captionTargetPageId="2" captionText="Fig. 1. Mesobiotus insanis, new species, PCM images of habitus and cuticle. A, dorso-ventral projection (holotype, PCM); B, dorsal cuticle above leg II; C, ventral cuticle of legs IV. Empty arrowheads indicate granulation; the filled arrowhead indicates the horse-shoe shaped structure connecting the anterior and the posterior claw. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357616" httpUri="https://zenodo.org/record/5357616/files/figure.png" pageId="2" pageNumber="442">Fig. 1B, C</figureCitation>
, empty arrowheads).
</paragraph>
<paragraph id="8BEE007FDB1DFFBB8C27F93AFE9BF913" blockId="2.[813,1452,1689,2097]" lastBlockId="4.[113,753,1202,1738]" lastPageId="4" lastPageNumber="444" pageId="2" pageNumber="442">
Buccal apparatus of 
<emphasis id="B925DC6DDB1DFFBD8B05F93AFB55F968" box="[1039,1184,1689,1713]" italics="true" pageId="2" pageNumber="442">Macrobiotus-</emphasis>
<typeStatus id="54EABEDDDB1DFFBD8BAAF93AFB3BF968" box="[1184,1230,1689,1713]" pageId="2" pageNumber="442">type</typeStatus>
with ventral lamina and ten peribuccal lamellae (
<figureCitation id="136A1CFADB1DFFBD8B69F91AFB4FF908" box="[1123,1210,1721,1745]" captionStart="Fig" captionStartId="3.[135,170,1958,1980]" captionTargetBox="[136,1474,213,1942]" captionTargetId="figure-8@3.[136,1474,213,1942]" captionTargetPageId="3" captionText="Fig. 2. Mesobiotus insanis, new species, PCM images of the buccal apparatus. A, an entire buccal apparatus (paratype); B, dorsal teeth; C–E, ventral teeth (in Fig C, D, E, the medio-ventral tooth is divided into three, four, and five oval teeth, respectively); F, ventral placoids; G, dorsal placoids. Filled flat arrowheads indicate the first band of teeth, empty flat arrowheads indicate the second band of teeth, filled indented arrowheads indicate the third band of teeth, empty arrows indicate the additional teeth in the third band, empty indented arrowheads indicate subterminal constrictions in the third macroplacoid. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357618" httpUri="https://zenodo.org/record/5357618/files/figure.png" pageId="2" pageNumber="442">Fig. 2A</figureCitation>
). Oral cavity armature of the 
<taxonomicName id="4C517BFCDB1DFFBD8C7EF97AFC09F928" baseAuthorityName="Murray" baseAuthorityYear="1907" box="[884,1020,1753,1777]" class="Eutardigrada" family="Macrobiotidae" genus="Mesobiotus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Parachela" pageId="2" pageNumber="442" phylum="Tardigrada" rank="species" species="harmsworthi">
<emphasis id="B925DC6DDB1DFFBD8C7EF97AFC09F928" box="[884,1020,1753,1777]" italics="true" pageId="2" pageNumber="442">harmsworthi</emphasis>
</taxonomicName>
<typeStatus id="54EABEDDDB1DFFBD8B0FF97AFBC3F928" box="[1029,1078,1753,1777]" pageId="2" pageNumber="442">type</typeStatus>
, composed of three bands of teeth visible under PCM (
<figureCitation id="136A1CFADB1DFFBD8B03F95AFBB9F8C8" box="[1033,1100,1785,1809]" captionStart="Fig" captionStartId="3.[135,170,1958,1980]" captionTargetBox="[136,1474,213,1942]" captionTargetId="figure-8@3.[136,1474,213,1942]" captionTargetPageId="3" captionText="Fig. 2. Mesobiotus insanis, new species, PCM images of the buccal apparatus. A, an entire buccal apparatus (paratype); B, dorsal teeth; C–E, ventral teeth (in Fig C, D, E, the medio-ventral tooth is divided into three, four, and five oval teeth, respectively); F, ventral placoids; G, dorsal placoids. Filled flat arrowheads indicate the first band of teeth, empty flat arrowheads indicate the second band of teeth, filled indented arrowheads indicate the third band of teeth, empty arrows indicate the additional teeth in the third band, empty indented arrowheads indicate subterminal constrictions in the third macroplacoid. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357618" httpUri="https://zenodo.org/record/5357618/files/figure.png" pageId="2" pageNumber="442">Fig. 2</figureCitation>
). The first band of teeth appears as small granules arranged in several irregular rows along the anterior portion of the oral cavity and sometimes on the bases of the lamellae (
<figureCitation id="136A1CFADB1DFFBD8B52F8FAFB3AF8A8" box="[1112,1231,1881,1905]" captionStart="Fig" captionStartId="3.[135,170,1958,1980]" captionTargetBox="[136,1474,213,1942]" captionTargetId="figure-8@3.[136,1474,213,1942]" captionTargetPageId="3" captionText="Fig. 2. Mesobiotus insanis, new species, PCM images of the buccal apparatus. A, an entire buccal apparatus (paratype); B, dorsal teeth; C–E, ventral teeth (in Fig C, D, E, the medio-ventral tooth is divided into three, four, and five oval teeth, respectively); F, ventral placoids; G, dorsal placoids. Filled flat arrowheads indicate the first band of teeth, empty flat arrowheads indicate the second band of teeth, filled indented arrowheads indicate the third band of teeth, empty arrows indicate the additional teeth in the third band, empty indented arrowheads indicate subterminal constrictions in the third macroplacoid. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357618" httpUri="https://zenodo.org/record/5357618/files/figure.png" pageId="2" pageNumber="442">Fig. 2B–E</figureCitation>
, filled arrowheads). Teeth in the second band appear as small ridges that are parallel to the main axis of the buccal tube and situated at the posterior portion of the oral cavity (
<figureCitation id="136A1CFADB1DFFBD8BEDF81AFAAEF808" box="[1255,1371,1977,2001]" captionStart="Fig" captionStartId="3.[135,170,1958,1980]" captionTargetBox="[136,1474,213,1942]" captionTargetId="figure-8@3.[136,1474,213,1942]" captionTargetPageId="3" captionText="Fig. 2. Mesobiotus insanis, new species, PCM images of the buccal apparatus. A, an entire buccal apparatus (paratype); B, dorsal teeth; C–E, ventral teeth (in Fig C, D, E, the medio-ventral tooth is divided into three, four, and five oval teeth, respectively); F, ventral placoids; G, dorsal placoids. Filled flat arrowheads indicate the first band of teeth, empty flat arrowheads indicate the second band of teeth, filled indented arrowheads indicate the third band of teeth, empty arrows indicate the additional teeth in the third band, empty indented arrowheads indicate subterminal constrictions in the third macroplacoid. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357618" httpUri="https://zenodo.org/record/5357618/files/figure.png" pageId="2" pageNumber="442">Fig. 2B–E</figureCitation>
, empty arrowheads). Situated right after the second band of teeth and before the buccal tube opening is the third band of teeth (
<figureCitation id="136A1CFADB1DFFBD8C3FF7BAFC51F7E8" box="[821,932,2073,2097]" captionStart="Fig" captionStartId="3.[135,170,1958,1980]" captionTargetBox="[136,1474,213,1942]" captionTargetId="figure-8@3.[136,1474,213,1942]" captionTargetPageId="3" captionText="Fig. 2. Mesobiotus insanis, new species, PCM images of the buccal apparatus. A, an entire buccal apparatus (paratype); B, dorsal teeth; C–E, ventral teeth (in Fig C, D, E, the medio-ventral tooth is divided into three, four, and five oval teeth, respectively); F, ventral placoids; G, dorsal placoids. Filled flat arrowheads indicate the first band of teeth, empty flat arrowheads indicate the second band of teeth, filled indented arrowheads indicate the third band of teeth, empty arrows indicate the additional teeth in the third band, empty indented arrowheads indicate subterminal constrictions in the third macroplacoid. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357618" httpUri="https://zenodo.org/record/5357618/files/figure.png" pageId="2" pageNumber="442">Fig. 2B–E</figureCitation>
, indented arrowheads), consisting of 3 dorsal thin ridge teeth and 5–7 ventral teeth: 2 drop-shaped lateral teeth and 3–5 oval median teeth sometimes with additional teeth (
<figureCitation id="136A1CFADB1BFFBB8F73FB51FF1EFAD3" box="[121,235,1266,1290]" captionStart="Fig" captionStartId="3.[135,170,1958,1980]" captionTargetBox="[136,1474,213,1942]" captionTargetId="figure-8@3.[136,1474,213,1942]" captionTargetPageId="3" captionText="Fig. 2. Mesobiotus insanis, new species, PCM images of the buccal apparatus. A, an entire buccal apparatus (paratype); B, dorsal teeth; C–E, ventral teeth (in Fig C, D, E, the medio-ventral tooth is divided into three, four, and five oval teeth, respectively); F, ventral placoids; G, dorsal placoids. Filled flat arrowheads indicate the first band of teeth, empty flat arrowheads indicate the second band of teeth, filled indented arrowheads indicate the third band of teeth, empty arrows indicate the additional teeth in the third band, empty indented arrowheads indicate subterminal constrictions in the third macroplacoid. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357618" httpUri="https://zenodo.org/record/5357618/files/figure.png" pageId="4" pageNumber="444">Fig. 2B–E</figureCitation>
, arrow). Buccal tube rigid, with a ventral lamina and a thickening posterior to the stylet support insertion points. Pharyngeal apophyses, three macroplacoids and a microplacoid present in the muscle pharynx. All placoids equidistant from each other. In the ventral view, the first and the third macroplacoid are rod-shaped whereas the second macroplacoid is drop-like. The first macroplacoid is thinner anteriorly whereas the third macroplacoid has a sub-terminal constriction, which is visible in both the ventral and the dorsal view (
<figureCitation id="136A1CFADB1BFFBB8EB0F9B1FDC0F9F3" box="[442,565,1554,1578]" captionStart="Fig" captionStartId="3.[135,170,1958,1980]" captionTargetBox="[136,1474,213,1942]" captionTargetId="figure-8@3.[136,1474,213,1942]" captionTargetPageId="3" captionText="Fig. 2. Mesobiotus insanis, new species, PCM images of the buccal apparatus. A, an entire buccal apparatus (paratype); B, dorsal teeth; C–E, ventral teeth (in Fig C, D, E, the medio-ventral tooth is divided into three, four, and five oval teeth, respectively); F, ventral placoids; G, dorsal placoids. Filled flat arrowheads indicate the first band of teeth, empty flat arrowheads indicate the second band of teeth, filled indented arrowheads indicate the third band of teeth, empty arrows indicate the additional teeth in the third band, empty indented arrowheads indicate subterminal constrictions in the third macroplacoid. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357618" httpUri="https://zenodo.org/record/5357618/files/figure.png" pageId="4" pageNumber="444">Fig. 2F, G</figureCitation>
, empty indented arrowheads). The macroplacoid sequence is 2&lt;1&lt;3. The drop-like microplacoid is typically longer than the second macroplacoid (93% of the analysed animals, in the remaining 7% the second macroplacoid is equal to or slightly longer than the microplacoid).
</paragraph>
<caption id="DF2E50F7DB1CFFBC8F8DF805FC7DF7F7" ID-DOI="http://doi.org/10.5281/zenodo.5357618" ID-Zenodo-Dep="5357618" httpUri="https://zenodo.org/record/5357618/files/figure.png" pageId="3" pageNumber="443" startId="3.[135,170,1958,1980]" targetBox="[136,1474,213,1942]" targetPageId="3">
<paragraph id="8BEE007FDB1CFFBC8F8DF805FC7DF7F7" blockId="3.[135,1474,1958,2095]" pageId="3" pageNumber="443">
Fig. 2. 
<taxonomicName id="4C517BFCDB1CFFBC8FC5F805FE7FF862" authority="Mapalo &amp; Stec &amp; Mirano-Bascos &amp; Michalczyk, 2017" authorityName="Mapalo &amp; Stec &amp; Mirano-Bascos &amp; Michalczyk" authorityYear="2017" box="[207,394,1958,1979]" class="Eutardigrada" family="Macrobiotidae" genus="Mesobiotus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Parachela" pageId="3" pageNumber="443" phylum="Tardigrada" rank="species" species="insanis" status="sp. nov.">
<emphasis id="B925DC6DDB1CFFBC8FC5F805FE7FF862" box="[207,394,1958,1979]" italics="true" pageId="3" pageNumber="443">Mesobiotus insanis</emphasis>
</taxonomicName>
, 
<taxonomicNameLabel id="A2166116DB1CFFBC8E92F805FDE5F865" box="[408,528,1958,1980]" pageId="3" pageNumber="443" rank="species">new species</taxonomicNameLabel>
, PCM images of the buccal apparatus. A, an entire buccal apparatus (paratype); B, dorsal teeth; C–E, ventral teeth (in Fig C, D, E, the medio-ventral tooth is divided into three, four, and five oval teeth, respectively); F, ventral placoids; G, dorsal placoids. Filled flat arrowheads indicate the first band of teeth, empty flat arrowheads indicate the second band of teeth, filled indented arrowheads indicate the third band of teeth, empty arrows indicate the additional teeth in the third band, empty indented arrowheads indicate subterminal constrictions in the third macroplacoid. Scale bars in μm.
</paragraph>
</caption>
<caption id="DF2E50F7DB1BFFBB8F7BFF1DFAD4FF0D" box="[113,1313,190,212]" pageId="4" pageNumber="444" startId="4.[113,168,190,212]">
<paragraph id="8BEE007FDB1BFFBB8F7BFF1DFAD4FF0D" blockId="4.[113,1313,190,212]" box="[113,1313,190,212]" pageId="4" pageNumber="444">
Table 1. Primers used for sequencing of 
<emphasis id="B925DC6DDB1BFFBB8D09FF1DFD93FF0A" box="[515,614,190,211]" italics="true" pageId="4" pageNumber="444">18S rRNA</emphasis>
, 
<emphasis id="B925DC6DDB1BFFBB8D79FF1DFD23FF0A" box="[627,726,190,211]" italics="true" pageId="4" pageNumber="444">28S rRNA</emphasis>
, 
<emphasis id="B925DC6DDB1BFFBB8DE9FF1DFCEDFF0A" box="[739,792,190,211]" italics="true" pageId="4" pageNumber="444">ITS-2</emphasis>
, and 
<emphasis id="B925DC6DDB1BFFBB8C5BFF1DFC8FFF0A" box="[849,890,190,211]" italics="true" pageId="4" pageNumber="444">COI</emphasis>
genes of 
<taxonomicName id="4C517BFCDB1BFFBB8CD6FF1DFB62FF0A" authority="Mapalo &amp; Stec &amp; Mirano-Bascos &amp; Michalczyk, 2017" authorityName="Mapalo &amp; Stec &amp; Mirano-Bascos &amp; Michalczyk" authorityYear="2017" box="[988,1175,190,211]" class="Eutardigrada" family="Macrobiotidae" genus="Mesobiotus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Parachela" pageId="4" pageNumber="444" phylum="Tardigrada" rank="species" species="insanis" status="sp. nov.">
<emphasis id="B925DC6DDB1BFFBB8CD6FF1DFB62FF0A" box="[988,1175,190,211]" italics="true" pageId="4" pageNumber="444">Mesobiotus insanis</emphasis>
</taxonomicName>
, 
<taxonomicNameLabel id="A2166116DB1BFFBB8BAFFF1DFAE9FF0D" box="[1189,1308,190,212]" pageId="4" pageNumber="444" rank="species">new species</taxonomicNameLabel>
.
</paragraph>
</caption>
<paragraph id="8BEE007FDB1BFFBB8F83FEA6FA67FD84" pageId="4" pageNumber="444">
<table id="F951F2DFDB1B00408F8AFEA6FA61FD84" box="[128,1428,261,605]" gridcols="5" gridrows="6" pageId="4" pageNumber="444">
<tr id="3561023DDB1B00408F8AFEA6FA61FEC2" box="[128,1428,261,283]" gridrow="0" pageId="4" pageNumber="444">
<th id="76B06B41DB1B00408F8AFEA6FEDCFEC2" box="[128,297,261,283]" gridcol="0" gridrow="0" pageId="4" pageNumber="444">
<emphasis id="B925DC6DDB1BFFBB8F83FEA6FEDCFEC3" bold="true" box="[137,297,261,282]" pageId="4" pageNumber="444">DNA Fragment</emphasis>
</th>
<th id="76B06B41DB1B00408E50FEA6FE12FEC2" box="[346,487,261,283]" gridcol="1" gridrow="0" pageId="4" pageNumber="444">
<emphasis id="B925DC6DDB1BFFBB8E50FEA6FE12FEC3" bold="true" box="[346,487,261,283]" pageId="4" pageNumber="444">Primer Name</emphasis>
</th>
<th id="76B06B41DB1B00408D19FEA6FD31FEC2" box="[531,708,261,283]" gridcol="2" gridrow="0" pageId="4" pageNumber="444">
<emphasis id="B925DC6DDB1BFFBB8D19FEA6FD31FEC2" bold="true" box="[531,708,261,283]" pageId="4" pageNumber="444">Primer Direction</emphasis>
</th>
<th id="76B06B41DB1B00408DFBFEA6FB7BFEC2" box="[753,1166,261,283]" gridcol="3" gridrow="0" pageId="4" pageNumber="444">
<emphasis id="B925DC6DDB1BFFBB8C49FEA6FBC9FEC2" bold="true" box="[835,1084,261,283]" pageId="4" pageNumber="444">Primer Sequence (5’-3’)</emphasis>
</th>
<th id="76B06B41DB1B00408BB5FEA6FA61FEC2" box="[1215,1428,261,283]" gridcol="4" gridrow="0" pageId="4" pageNumber="444">
<emphasis id="B925DC6DDB1BFFBB8A0CFEA6FAB8FEC2" bold="true" box="[1286,1357,261,283]" pageId="4" pageNumber="444">Source</emphasis>
</th>
</tr>
<tr id="3561023DDB1B00408F8AFE9FFA61FEAB" box="[128,1428,316,370]" gridrow="1" pageId="4" pageNumber="444">
<th id="76B06B41DB1B00408F8AFE9FFEDCFEAB" box="[128,297,316,370]" gridcol="0" gridrow="1" pageId="4" pageNumber="444">18S rRNA</th>
<td id="76B06B41DB1B00408E50FE9FFE12FEAB" box="[346,487,316,370]" gridcol="1" gridrow="1" pageId="4" pageNumber="444">SSU F7 SSU R9</td>
<td id="76B06B41DB1B00408D19FE9FFD31FEAB" box="[531,708,316,370]" gridcol="2" gridrow="1" pageId="4" pageNumber="444">forward reverse</td>
<td id="76B06B41DB1B00408DFBFE9FFB7BFEAB" box="[753,1166,316,370]" gridcol="3" gridrow="1" pageId="4" pageNumber="444">AAAGATTAAGCCATGCAT AGCTGGAATTACCGCGGCTG</td>
<td id="76B06B41DB1B00408BB5FE9FFA61FEAB" box="[1215,1428,316,370]" gridcol="4" gridrow="1" pageId="4" pageNumber="444">
<bibRefCitation id="EFC07D8EDB1BFFBB8BCCFE9FFA78FE8B" author="Blaxter M &amp; De Ley P &amp; Garey JR &amp; Liu LX &amp; Scheldeman P &amp; Vierstraete A &amp; Vanfleteren JR &amp; Mackey LY &amp; Dorris M &amp; Frisse LM &amp; Vida JT &amp; Thomas WK" box="[1222,1421,316,338]" pageId="4" pageNumber="444" pagination="71 - 75" refId="ref7556" refString="Blaxter M, De Ley P, Garey JR, Liu LX, Scheldeman P, Vierstraete A, Vanfleteren JR, Mackey LY, Dorris M, Frisse LM, Vida JT, &amp; Thomas WK (1998) A molecular evolutionary framework for the phylum Nematoda. Nature, 392 (6671): 71 - 75. https: // dx. doi. org / 10.1038 / 32160." type="journal article" year="1998">Blaxter et al. (1998)</bibRefCitation>
<bibRefCitation id="EFC07D8EDB1BFFBB8BCCFEF8FA78FEAB" author="Blaxter M &amp; De Ley P &amp; Garey JR &amp; Liu LX &amp; Scheldeman P &amp; Vierstraete A &amp; Vanfleteren JR &amp; Mackey LY &amp; Dorris M &amp; Frisse LM &amp; Vida JT &amp; Thomas WK" box="[1222,1421,347,370]" pageId="4" pageNumber="444" pagination="71 - 75" refId="ref7556" refString="Blaxter M, De Ley P, Garey JR, Liu LX, Scheldeman P, Vierstraete A, Vanfleteren JR, Mackey LY, Dorris M, Frisse LM, Vida JT, &amp; Thomas WK (1998) A molecular evolutionary framework for the phylum Nematoda. Nature, 392 (6671): 71 - 75. https: // dx. doi. org / 10.1038 / 32160." type="journal article" year="1998">Blaxter et al. (1998)</bibRefCitation>
</td>
</tr>
<tr id="3561023DDB1B00408F8AFE29FA61FE19" box="[128,1428,394,448]" gridrow="2" pageId="4" pageNumber="444">
<th id="76B06B41DB1B00408F8AFE29FEDCFE19" box="[128,297,394,448]" gridcol="0" gridrow="2" pageId="4" pageNumber="444">28S rRNA</th>
<td id="76B06B41DB1B00408E50FE29FE12FE19" box="[346,487,394,448]" gridcol="1" gridrow="2" pageId="4" pageNumber="444">28SF0001 28SR0990</td>
<td id="76B06B41DB1B00408D19FE29FD31FE19" box="[531,708,394,448]" gridcol="2" gridrow="2" pageId="4" pageNumber="444">forward reverse</td>
<td id="76B06B41DB1B00408DFBFE29FB7BFE19" box="[753,1166,394,448]" gridcol="3" gridrow="2" pageId="4" pageNumber="444">ACCCVCYNAATTTAAGCATAT CCTTGGTCCGTGTTTCAAGAC</td>
<td id="76B06B41DB1B00408BB5FE29FA61FE19" box="[1215,1428,394,448]" gridcol="4" gridrow="2" pageId="4" pageNumber="444">
<bibRefCitation id="EFC07D8EDB1BFFBB8BB5FE29FA61FE79" author="Mironov SV &amp; Dabert J &amp; Dabert M." box="[1215,1428,394,416]" pageId="4" pageNumber="444" pagination="54 - 61" refId="ref8269" refString="Mironov SV, Dabert J &amp; Dabert M. (2012) A new feather mite species of the genus Proctophyllodes Robin, 1877 (Astigmata: Proctophyllodidae) from the Long-tailed Tit Aegithalos caudatus (Passeriformes: Aegithalidae): morphological description with DNA barcode data. Zootaxa, 3253: 54 - 61." type="journal article" year="2012">Mironov et al. (2012)</bibRefCitation>
<bibRefCitation id="EFC07D8EDB1BFFBB8BB5FE09FA61FE19" author="Mironov SV &amp; Dabert J &amp; Dabert M." box="[1215,1428,426,448]" pageId="4" pageNumber="444" pagination="54 - 61" refId="ref8269" refString="Mironov SV, Dabert J &amp; Dabert M. (2012) A new feather mite species of the genus Proctophyllodes Robin, 1877 (Astigmata: Proctophyllodidae) from the Long-tailed Tit Aegithalos caudatus (Passeriformes: Aegithalidae): morphological description with DNA barcode data. Zootaxa, 3253: 54 - 61." type="journal article" year="2012">Mironov et al. (2012)</bibRefCitation>
</td>
</tr>
<tr id="3561023DDB1B00408F8AFE7AFA61FDD6" box="[128,1428,473,527]" gridrow="3" pageId="4" pageNumber="444">
<th id="76B06B41DB1B00408F8AFE7AFEDCFDD6" box="[128,297,473,527]" gridcol="0" gridrow="3" pageId="4" pageNumber="444">ITS-2</th>
<td id="76B06B41DB1B00408E50FE7AFE12FDD6" box="[346,487,473,527]" gridcol="1" gridrow="3" pageId="4" pageNumber="444">ITS3 ITS4</td>
<td id="76B06B41DB1B00408D19FE7AFD31FDD6" box="[531,708,473,527]" gridcol="2" gridrow="3" pageId="4" pageNumber="444">forward reverse</td>
<td id="76B06B41DB1B00408DFBFE7AFB7BFDD6" box="[753,1166,473,527]" gridcol="3" gridrow="3" pageId="4" pageNumber="444">GCATCGATGAAGAACGCAGC TCCTCCGCTTATTGATATGC</td>
<td id="76B06B41DB1B00408BB5FE7AFA61FDD6" box="[1215,1428,473,527]" gridcol="4" gridrow="3" pageId="4" pageNumber="444">
<bibRefCitation id="EFC07D8EDB1BFFBB8BC6FE7AFA72FE36" author="White TJ &amp; Bruns T &amp; Lee S &amp; Taylor J" box="[1228,1415,473,495]" pageId="4" pageNumber="444" pagination="315 - 322" refId="ref9277" refString="White TJ, Bruns T, Lee S &amp; Taylor J (1990) Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In: Innis MA, Gelfand DH &amp; Sninsky JJ (eds.) PCR Protocols: A Guide to Methods and Applications. Academic Press Inc., New York. Pp. 315 - 322." type="book chapter" year="1990">White et al. (1990)</bibRefCitation>
<bibRefCitation id="EFC07D8EDB1BFFBB8BC6FE5AFA72FDD6" author="White TJ &amp; Bruns T &amp; Lee S &amp; Taylor J" box="[1228,1415,505,527]" pageId="4" pageNumber="444" pagination="315 - 322" refId="ref9277" refString="White TJ, Bruns T, Lee S &amp; Taylor J (1990) Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In: Innis MA, Gelfand DH &amp; Sninsky JJ (eds.) PCR Protocols: A Guide to Methods and Applications. Academic Press Inc., New York. Pp. 315 - 322." type="book chapter" year="1990">White et al. (1990)</bibRefCitation>
</td>
</tr>
<tr id="3561023DDB1B00408F8AFD83FA61FDE4" box="[128,1428,544,573]" gridrow="4" pageId="4" pageNumber="444">
<th id="76B06B41DB1B00408F8AFD83FEDCFDE4" box="[128,297,544,573]" gridcol="0" gridrow="4" pageId="4" pageNumber="444">COI</th>
<td id="76B06B41DB1B00408E50FD83FE12FDE4" box="[346,487,544,573]" gridcol="1" gridrow="4" pageId="4" pageNumber="444">LCO1490</td>
<td id="76B06B41DB1B00408D19FD83FD31FDE4" box="[531,708,544,573]" gridcol="2" gridrow="4" pageId="4" pageNumber="444">forward</td>
<td id="76B06B41DB1B00408DFBFD83FB7BFDE4" box="[753,1166,544,573]" gridcol="3" gridrow="4" pageId="4" pageNumber="444">GGTCAACAAATCATAAAGATATTGG</td>
<td id="76B06B41DB1B00408BB5FD83FA61FDE4" box="[1215,1428,544,573]" gridcol="4" gridrow="4" pageId="4" pageNumber="444">
<bibRefCitation id="EFC07D8EDB1BFFBB8BCDFD83FA79FDEF" author="Folmer O &amp; Black M &amp; Hoeh W &amp; Lutz R &amp; Vrijenhoek R" box="[1223,1420,544,566]" pageId="4" pageNumber="444" pagination="294 - 299" refId="ref7769" refString="Folmer O, Black M, Hoeh W, Lutz R &amp; Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294 - 299." type="journal article" year="1994">Folmer et al. (1994)</bibRefCitation>
</td>
</tr>
<tr id="3561023DDB1B00408F8AFDE4FA61FD84" box="[128,1428,583,605]" gridrow="5" pageId="4" pageNumber="444" rowspan-0="1">
<td id="76B06B41DB1B00408E50FDE4FE12FD84" box="[346,487,583,605]" gridcol="1" gridrow="5" pageId="4" pageNumber="444">HCOoutout</td>
<td id="76B06B41DB1B00408D19FDE4FD31FD84" box="[531,708,583,605]" gridcol="2" gridrow="5" pageId="4" pageNumber="444">reverse</td>
<td id="76B06B41DB1B00408DFBFDE4FB7BFD84" box="[753,1166,583,605]" gridcol="3" gridrow="5" pageId="4" pageNumber="444">GTAAATATATGRTGDGCTC</td>
<td id="76B06B41DB1B00408BB5FDE4FA61FD84" box="[1215,1428,583,605]" gridcol="4" gridrow="5" pageId="4" pageNumber="444">
<bibRefCitation id="EFC07D8EDB1BFFBB8BCBFDE4FA67FD84" author="Prendini L &amp; Weygoldt P &amp; Wheeler WC" box="[1217,1426,583,605]" pageId="4" pageNumber="444" pagination="203 - 236" refId="ref8658" refString="Prendini L, Weygoldt P &amp; Wheeler WC (2005) Systematics of the Damon variegatus group of African whip spiders (Chelicerata: Amblypygi): evidence from behaviour, morphology and DNA. Organisms, Diversity and Evolution, 5: 203 - 236." type="journal article" year="2005">Prendini et al. (2005)</bibRefCitation>
</td>
</tr>
</table>
</paragraph>
<caption id="DF2E50F7DB1BFFBB8F7BFB88FB1BFBA3" ID-DOI="http://doi.org/10.5281/zenodo.5357620" ID-Zenodo-Dep="5357620" httpUri="https://zenodo.org/record/5357620/files/figure.png" pageId="4" pageNumber="444" startId="4.[113,148,1067,1089]" targetBox="[113,1451,668,1051]" targetPageId="4">
<paragraph id="8BEE007FDB1BFFBB8F7BFB88FB1BFBA3" blockId="4.[113,1452,1067,1147]" pageId="4" pageNumber="444">
Fig. 3. 
<taxonomicName id="4C517BFCDB1BFFBB8FBCFB88FE9AFB98" authority="Mapalo &amp; Stec &amp; Mirano-Bascos &amp; Michalczyk, 2017" authorityName="Mapalo &amp; Stec &amp; Mirano-Bascos &amp; Michalczyk" authorityYear="2017" box="[182,367,1067,1089]" class="Eutardigrada" family="Macrobiotidae" genus="Mesobiotus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Parachela" pageId="4" pageNumber="444" phylum="Tardigrada" rank="species" species="insanis" status="sp. nov.">
<emphasis id="B925DC6DDB1BFFBB8FBCFB88FE9AFB98" box="[182,367,1067,1089]" italics="true" pageId="4" pageNumber="444">Mesobiotus insanis</emphasis>
</taxonomicName>
, 
<taxonomicNameLabel id="A2166116DB1BFFBB8E76FB8FFE07FB98" box="[380,498,1067,1089]" pageId="4" pageNumber="444" rank="species">new species</taxonomicNameLabel>
, PCM images of claws. A, claws I with smooth lunules; B, claws II with smooth lunules; C, claws IV with slightly crenulated lunules. Arrows indicate the W-shaped cuticular bars, the filled arrowhead indicates the horseshoe-shaped structure connecting the anterior and the posterior claw, the empty arrowheads indicate leg granulation. Scale in μm.
</paragraph>
</caption>
<paragraph id="8BEE007FDB1BFFBB8F7BF951FF08F7D3" blockId="4.[113,753,1778,2058]" pageId="4" pageNumber="444">
Claws of the 
<taxonomicName id="4C517BFCDB1BFFBB8E0FF951FE75F8D3" authorityName="Vecchi, Cesari, Bertolani, Jonsson, Rebecchi &amp; Guidetti" authorityYear="2016" box="[261,384,1778,1802]" class="Eutardigrada" family="Macrobiotidae" genus="Mesobiotus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Parachela" pageId="4" pageNumber="444" phylum="Tardigrada" rank="genus">
<emphasis id="B925DC6DDB1BFFBB8E0FF951FE75F8D3" box="[261,384,1778,1802]" italics="true" pageId="4" pageNumber="444">Mesobiotus</emphasis>
</taxonomicName>
<typeStatus id="54EABEDDDB1BFFBB8E82F950FE4CF8D2" box="[392,441,1779,1803]" pageId="4" pageNumber="444">type</typeStatus>
, with a peduncle connecting the claw to the lunula, a basal septum and well-developed accessory points on the primary branches (
<figureCitation id="136A1CFADB1BFFBB8D6CF891FD16F893" box="[614,739,1842,1866]" captionStart="Fig" captionStartId="4.[113,148,1067,1089]" captionTargetBox="[113,1451,668,1051]" captionTargetId="figure-130@4.[113,1451,668,1051]" captionTargetPageId="4" captionText="Fig. 3. Mesobiotus insanis, new species, PCM images of claws. A, claws I with smooth lunules; B, claws II with smooth lunules; C, claws IV with slightly crenulated lunules. Arrows indicate the W-shaped cuticular bars, the filled arrowhead indicates the horseshoe-shaped structure connecting the anterior and the posterior claw, the empty arrowheads indicate leg granulation. Scale in μm." figureDoi="http://doi.org/10.5281/zenodo.5357620" httpUri="https://zenodo.org/record/5357620/files/figure.png" pageId="4" pageNumber="444">Fig. 3A–C</figureCitation>
). Lunules smooth under claws I–III (
<figureCitation id="136A1CFADB1BFFBB8EE5F8F1FD97F8B3" box="[495,610,1874,1898]" captionStart="Fig" captionStartId="4.[113,148,1067,1089]" captionTargetBox="[113,1451,668,1051]" captionTargetId="figure-130@4.[113,1451,668,1051]" captionTargetPageId="4" captionText="Fig. 3. Mesobiotus insanis, new species, PCM images of claws. A, claws I with smooth lunules; B, claws II with smooth lunules; C, claws IV with slightly crenulated lunules. Arrows indicate the W-shaped cuticular bars, the filled arrowhead indicates the horseshoe-shaped structure connecting the anterior and the posterior claw, the empty arrowheads indicate leg granulation. Scale in μm." figureDoi="http://doi.org/10.5281/zenodo.5357620" httpUri="https://zenodo.org/record/5357620/files/figure.png" pageId="4" pageNumber="444">Fig. 3A, B</figureCitation>
) and slightly crenulated under claws IV (
<figureCitation id="136A1CFADB1BFFBB8EBDF8D1FDE6F853" box="[439,531,1906,1930]" captionStart="Fig" captionStartId="4.[113,148,1067,1089]" captionTargetBox="[113,1451,668,1051]" captionTargetId="figure-130@4.[113,1451,668,1051]" captionTargetPageId="4" captionText="Fig. 3. Mesobiotus insanis, new species, PCM images of claws. A, claws I with smooth lunules; B, claws II with smooth lunules; C, claws IV with slightly crenulated lunules. Arrows indicate the W-shaped cuticular bars, the filled arrowhead indicates the horseshoe-shaped structure connecting the anterior and the posterior claw, the empty arrowheads indicate leg granulation. Scale in μm." figureDoi="http://doi.org/10.5281/zenodo.5357620" httpUri="https://zenodo.org/record/5357620/files/figure.png" pageId="4" pageNumber="444">Fig. 3C</figureCitation>
). Single transverse W-shaped bars below claws I–III present (
<figureCitation id="136A1CFADB1BFFBB8D4BF831FD63F873" box="[577,662,1938,1962]" captionStart="Fig" captionStartId="4.[113,148,1067,1089]" captionTargetBox="[113,1451,668,1051]" captionTargetId="figure-130@4.[113,1451,668,1051]" captionTargetPageId="4" captionText="Fig. 3. Mesobiotus insanis, new species, PCM images of claws. A, claws I with smooth lunules; B, claws II with smooth lunules; C, claws IV with slightly crenulated lunules. Arrows indicate the W-shaped cuticular bars, the filled arrowhead indicates the horseshoe-shaped structure connecting the anterior and the posterior claw, the empty arrowheads indicate leg granulation. Scale in μm." figureDoi="http://doi.org/10.5281/zenodo.5357620" httpUri="https://zenodo.org/record/5357620/files/figure.png" pageId="4" pageNumber="444">Fig. 3B</figureCitation>
, arrow), whereas a horseshoe-shaped structure connects the anterior and posterior lunules on claws IV (
<figureCitation id="136A1CFADB1BFFBB8D04F871FD86F833" box="[526,627,2002,2026]" captionStart="Fig" captionStartId="2.[113,148,1170,1192]" captionTargetBox="[113,1451,189,1155]" captionTargetId="figure-311@2.[113,1451,189,1155]" captionTargetPageId="2" captionText="Fig. 1. Mesobiotus insanis, new species, PCM images of habitus and cuticle. A, dorso-ventral projection (holotype, PCM); B, dorsal cuticle above leg II; C, ventral cuticle of legs IV. Empty arrowheads indicate granulation; the filled arrowhead indicates the horse-shoe shaped structure connecting the anterior and the posterior claw. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357616" httpUri="https://zenodo.org/record/5357616/files/figure.png" pageId="4" pageNumber="444">Figs. 1C</figureCitation>
, 
<figureCitation id="136A1CFADB1BFFBB8D89F871FD53F833" box="[643,678,2002,2026]" captionStart="Fig" captionStartId="4.[113,148,1067,1089]" captionTargetBox="[113,1451,668,1051]" captionTargetId="figure-130@4.[113,1451,668,1051]" captionTargetPageId="4" captionText="Fig. 3. Mesobiotus insanis, new species, PCM images of claws. A, claws I with smooth lunules; B, claws II with smooth lunules; C, claws IV with slightly crenulated lunules. Arrows indicate the W-shaped cuticular bars, the filled arrowhead indicates the horseshoe-shaped structure connecting the anterior and the posterior claw, the empty arrowheads indicate leg granulation. Scale in μm." figureDoi="http://doi.org/10.5281/zenodo.5357620" httpUri="https://zenodo.org/record/5357620/files/figure.png" pageId="4" pageNumber="444">3C</figureCitation>
, filled arrowheads).
</paragraph>
<paragraph id="8BEE007FDB1BFFBB8C27FB10FA59F7D3" blockId="4.[812,1453,1202,2058]" pageId="4" pageNumber="444">
Eggs (morphometrics in 
<tableCitation id="C6D335C4DB1BFFBB8B42FB11FB54FB13" box="[1096,1185,1202,1226]" captionStart="Table 5" captionStartId="9.[135,190,189,211]" captionText="Table 5. Measurements (in μm) of selected morphological structures of eggs of Mesobiotus insanis, new species. mounted in Hoyer’s medium (N—number of eggs/structures measured, Range refers to the smallest and the largest structure among all measured specimens; SD—standard deviation)." pageId="4" pageNumber="444">Table 5</tableCitation>
): Spherical, white, laid freely, with hemispherical to conical processes. The processes are equidistant from each other and vary in shape from low domes to high cones (
<figureCitation id="136A1CFADB1BFFBB8B2FFAB1FB6BFAF3" box="[1061,1182,1298,1322]" captionStart="Fig" captionStartId="9.[136,171,1673,1695]" captionTargetBox="[135,1474,664,1657]" captionTargetId="figure-359@9.[135,1474,664,1657]" captionTargetPageId="9" captionText="Fig. 6. Mesobiotus insanis, new species, PCM images of the midsection of processes from various eggs. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357626" httpUri="https://zenodo.org/record/5357626/files/figure.png" pageId="4" pageNumber="444">Fig. 6A–L</figureCitation>
). The process surface is wrinkled and the wrinkles form a whorl that is clearly visible with SEM (
<figureCitation id="136A1CFADB1BFFBB8CBBFAF1FBD3FAB3" box="[945,1062,1362,1386]" captionStart="Fig" captionStartId="11.[135,170,1987,2009]" captionTargetBox="[136,1474,237,1971]" captionTargetId="figure-72@11.[136,1474,237,1971]" captionTargetPageId="11" captionText="Fig. 8. Mesobiotus insanis, new species, SEM images of eggs. A, B, entire view of two different eggs; C, D, egg surface with processes; E, egg process; F, egg surface between processes, with evident pores and whorl-shaped sculpturing. Arrows indicate pores in the processes, flat arrowheads indicate the whorl-shaped surface inside the areolae, indented arrowheads indicate pores inside the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357630" httpUri="https://zenodo.org/record/5357630/files/figure.png" pageId="4" pageNumber="444">Fig. 8A–F</figureCitation>
) but only appears as serrations on intersected process walls under PCM (
<figureCitation id="136A1CFADB1BFFBB8BD6FAD1FAACFA53" box="[1244,1369,1394,1418]" captionStart="Fig" captionStartId="9.[136,171,1673,1695]" captionTargetBox="[135,1474,664,1657]" captionTargetId="figure-359@9.[135,1474,664,1657]" captionTargetPageId="9" captionText="Fig. 6. Mesobiotus insanis, new species, PCM images of the midsection of processes from various eggs. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357626" httpUri="https://zenodo.org/record/5357626/files/figure.png" pageId="4" pageNumber="444">Fig. 6A–H</figureCitation>
). Some eggs have smooth process surfaces under PCM (
<figureCitation id="136A1CFADB1BFFBB8A3CFA31FA55FA73" box="[1334,1440,1426,1450]" captionStart="Fig" captionStartId="9.[136,171,1673,1695]" captionTargetBox="[135,1474,664,1657]" captionTargetId="figure-359@9.[135,1474,664,1657]" captionTargetPageId="9" captionText="Fig. 6. Mesobiotus insanis, new species, PCM images of the midsection of processes from various eggs. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357626" httpUri="https://zenodo.org/record/5357626/files/figure.png" pageId="4" pageNumber="444">Fig. 6I–L</figureCitation>
). The labyrinthine layer within the process walls appears as reticulation under PCM, with meshes that vary in diameter considerably between eggs (
<figureCitation id="136A1CFADB1BFFBB8B63FA51FB13F9D3" box="[1129,1254,1522,1546]" captionStart="Fig" captionStartId="10.[113,148,2036,2058]" captionTargetBox="[134,1430,189,2020]" captionTargetId="figure-11@10.[134,1430,189,2020]" captionTargetPageId="10" captionText="Fig. 7. Mesobiotus insanis, new species, magnified PCM images of the surface of the eggs shown in Fig. 4 (respective letters indicate same eggs in both figures). Filled arrowheads indicate the whorled sculpturing inside areolae; empty arrowheads indicate the bubble-like structures within the rims delimiting the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357628" httpUri="https://zenodo.org/record/5357628/files/figure.png" pageId="4" pageNumber="444">Fig. 7A–D</figureCitation>
). A few scattered pores, especially in the basal portion of the process, are present in the external process wall (only clearly visible in SEM since in PCM the pores blend with the labyrinthine layer). Processes are terminated by at least 15 short, thin, and flexible filaments that are visible in both PCM (
<figureCitation id="136A1CFADB1BFFBB8A75F931FC98F913" captionStart="Fig" captionStartId="9.[136,171,1673,1695]" captionTargetBox="[135,1474,664,1657]" captionTargetId="figure-359@9.[135,1474,664,1657]" captionTargetPageId="9" captionText="Fig. 6. Mesobiotus insanis, new species, PCM images of the midsection of processes from various eggs. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357626" httpUri="https://zenodo.org/record/5357626/files/figure.png" pageId="4" pageNumber="444">Fig. 6A–L</figureCitation>
) and SEM (
<figureCitation id="136A1CFADB1BFFBB8CF0F911FB86F913" box="[1018,1139,1714,1738]" captionStart="Fig" captionStartId="11.[135,170,1987,2009]" captionTargetBox="[136,1474,237,1971]" captionTargetId="figure-72@11.[136,1474,237,1971]" captionTargetPageId="11" captionText="Fig. 8. Mesobiotus insanis, new species, SEM images of eggs. A, B, entire view of two different eggs; C, D, egg surface with processes; E, egg process; F, egg surface between processes, with evident pores and whorl-shaped sculpturing. Arrows indicate pores in the processes, flat arrowheads indicate the whorl-shaped surface inside the areolae, indented arrowheads indicate pores inside the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357630" httpUri="https://zenodo.org/record/5357630/files/figure.png" pageId="4" pageNumber="444">Fig. 8A–F</figureCitation>
). The filaments are covered with tiny granules that are visible only in SEM (
<figureCitation id="136A1CFADB1BFFBB8A45F971FCB4F8D3" captionStart="Fig" captionStartId="11.[135,170,1987,2009]" captionTargetBox="[136,1474,237,1971]" captionTargetId="figure-72@11.[136,1474,237,1971]" captionTargetPageId="11" captionText="Fig. 8. Mesobiotus insanis, new species, SEM images of eggs. A, B, entire view of two different eggs; C, D, egg surface with processes; E, egg process; F, egg surface between processes, with evident pores and whorl-shaped sculpturing. Arrows indicate pores in the processes, flat arrowheads indicate the whorl-shaped surface inside the areolae, indented arrowheads indicate pores inside the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357630" httpUri="https://zenodo.org/record/5357630/files/figure.png" pageId="4" pageNumber="444">Fig. 8D, E</figureCitation>
). Processes are connected by 10–14 wide stripes (
<figureCitation id="136A1CFADB1BFFBB8A50F951FA53F8D3" box="[1370,1446,1778,1802]" captionStart="Fig" captionStartId="10.[113,148,2036,2058]" captionTargetBox="[134,1430,189,2020]" captionTargetId="figure-11@10.[134,1430,189,2020]" captionTargetPageId="10" captionText="Fig. 7. Mesobiotus insanis, new species, magnified PCM images of the surface of the eggs shown in Fig. 4 (respective letters indicate same eggs in both figures). Filled arrowheads indicate the whorled sculpturing inside areolae; empty arrowheads indicate the bubble-like structures within the rims delimiting the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357628" httpUri="https://zenodo.org/record/5357628/files/figure.png" pageId="4" pageNumber="444">Figs. 7</figureCitation>
, 
<figureCitation id="136A1CFADB1BFFBB8C27F8B1FCBBF8F3" box="[813,846,1810,1834]" captionStart="Fig" captionStartId="11.[135,170,1987,2009]" captionTargetBox="[136,1474,237,1971]" captionTargetId="figure-72@11.[136,1474,237,1971]" captionTargetPageId="11" captionText="Fig. 8. Mesobiotus insanis, new species, SEM images of eggs. A, B, entire view of two different eggs; C, D, egg surface with processes; E, egg process; F, egg surface between processes, with evident pores and whorl-shaped sculpturing. Arrows indicate pores in the processes, flat arrowheads indicate the whorl-shaped surface inside the areolae, indented arrowheads indicate pores inside the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357630" httpUri="https://zenodo.org/record/5357630/files/figure.png" pageId="4" pageNumber="444">8A</figureCitation>
) with smooth surfaces in SEM (
<figureCitation id="136A1CFADB1BFFBB8BBBF8B1FB04F8F3" box="[1201,1265,1810,1834]" captionStart="Fig" captionStartId="11.[135,170,1987,2009]" captionTargetBox="[136,1474,237,1971]" captionTargetId="figure-72@11.[136,1474,237,1971]" captionTargetPageId="11" captionText="Fig. 8. Mesobiotus insanis, new species, SEM images of eggs. A, B, entire view of two different eggs; C, D, egg surface with processes; E, egg process; F, egg surface between processes, with evident pores and whorl-shaped sculpturing. Arrows indicate pores in the processes, flat arrowheads indicate the whorl-shaped surface inside the areolae, indented arrowheads indicate pores inside the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357630" httpUri="https://zenodo.org/record/5357630/files/figure.png" pageId="4" pageNumber="444">Fig. 8</figureCitation>
) but in PCM the stripes appear covered with mesh (
<figureCitation id="136A1CFADB1BFFBB8BB8F891FB03F893" box="[1202,1270,1842,1866]" captionStart="Fig" captionStartId="10.[113,148,2036,2058]" captionTargetBox="[134,1430,189,2020]" captionTargetId="figure-11@10.[134,1430,189,2020]" captionTargetPageId="10" captionText="Fig. 7. Mesobiotus insanis, new species, magnified PCM images of the surface of the eggs shown in Fig. 4 (respective letters indicate same eggs in both figures). Filled arrowheads indicate the whorled sculpturing inside areolae; empty arrowheads indicate the bubble-like structures within the rims delimiting the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357628" httpUri="https://zenodo.org/record/5357628/files/figure.png" pageId="4" pageNumber="444">Fig. 7</figureCitation>
) and sometimes large bubbles (
<figureCitation id="136A1CFADB1BFFBB8CC0F8F1FBEAF8B3" box="[970,1055,1874,1898]" captionStart="Fig" captionStartId="10.[113,148,2036,2058]" captionTargetBox="[134,1430,189,2020]" captionTargetId="figure-11@10.[134,1430,189,2020]" captionTargetPageId="10" captionText="Fig. 7. Mesobiotus insanis, new species, magnified PCM images of the surface of the eggs shown in Fig. 4 (respective letters indicate same eggs in both figures). Filled arrowheads indicate the whorled sculpturing inside areolae; empty arrowheads indicate the bubble-like structures within the rims delimiting the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357628" httpUri="https://zenodo.org/record/5357628/files/figure.png" pageId="4" pageNumber="444">Fig. 7D</figureCitation>
, empty arrowhead), which are all the representations of the labyrinthine layer below the surface of the stripes. The spaces between the connective stripes form single, complexly sculptured areolae on the egg surface (10–14 areolae around each process; 
<figureCitation id="136A1CFADB1BFFBB8BD2F871FADDF833" box="[1240,1320,2002,2026]" captionStart="Fig" captionStartId="10.[113,148,2036,2058]" captionTargetBox="[134,1430,189,2020]" captionTargetId="figure-11@10.[134,1430,189,2020]" captionTargetPageId="10" captionText="Fig. 7. Mesobiotus insanis, new species, magnified PCM images of the surface of the eggs shown in Fig. 4 (respective letters indicate same eggs in both figures). Filled arrowheads indicate the whorled sculpturing inside areolae; empty arrowheads indicate the bubble-like structures within the rims delimiting the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357628" httpUri="https://zenodo.org/record/5357628/files/figure.png" pageId="4" pageNumber="444">Figs. 7</figureCitation>
, 
<figureCitation id="136A1CFADB1BFFBB8A32F871FA6BF833" box="[1336,1438,2002,2026]" captionStart="Fig" captionStartId="11.[135,170,1987,2009]" captionTargetBox="[136,1474,237,1971]" captionTargetId="figure-72@11.[136,1474,237,1971]" captionTargetPageId="11" captionText="Fig. 8. Mesobiotus insanis, new species, SEM images of eggs. A, B, entire view of two different eggs; C, D, egg surface with processes; E, egg process; F, egg surface between processes, with evident pores and whorl-shaped sculpturing. Arrows indicate pores in the processes, flat arrowheads indicate the whorl-shaped surface inside the areolae, indented arrowheads indicate pores inside the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357630" httpUri="https://zenodo.org/record/5357630/files/figure.png" pageId="4" pageNumber="444">8A–D, F</figureCitation>
). Each areola consists of a central rose-shaped whorl of fine
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E99FF41FE4DFEBE" blockId="5.[270,621,226,2111]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E99FF41FE5FFEFC" bold="true" box="[403,426,226,293]" pageId="5" pageNumber="445">Cycles</emphasis>
1 35 35 35 1
<emphasis id="B925DC6DDB1AFFBA8EABFEC2FE4DFEBE" bold="true" box="[417,440,353,359]" pageId="5" pageNumber="445">]</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E5CFEDAFE4DFE54" blockId="5.[270,621,226,2111]" box="[342,440,373,441]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E5CFEDAFE4DFE54" bold="true" box="[342,440,373,441]" pageId="5" pageNumber="445">COI Time min:</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8EABFEC4FE4DFE66" blockId="5.[270,621,226,2111]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8EABFEC4FE4DFE51" bold="true" box="[417,440,359,392]" pageId="5" pageNumber="445">sec</emphasis>
00 10:: 00 30 00 30:: 01 00: 00 10 
<emphasis id="B925DC6DDB1AFFBA8EABFE1AFE4DFE66" bold="true" box="[417,440,441,447]" pageId="5" pageNumber="445">[</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E99FE46FE5FFE35" blockId="5.[270,621,226,2111]" box="[403,426,485,492]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E99FE46FE5FFE35" bold="true" box="[403,426,485,492]" pageId="5" pageNumber="445">]</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E99FE4EFE5FFDD8" blockId="5.[270,621,226,2111]" box="[403,426,493,513]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E99FE4EFE5FFDD8" bold="true" box="[403,426,493,513]" pageId="5" pageNumber="445">C</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E99FDA3FE5FFDD0" blockId="5.[270,621,226,2111]" box="[403,426,512,521]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E99FDA3FE5FFDD0" bold="true" box="[403,426,512,521]" pageId="5" pageNumber="445">°</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E99FDAAFE5FFD8D" blockId="5.[270,621,226,2111]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E99FDAAFE5FFDC9" bold="true" box="[403,426,521,528]" pageId="5" pageNumber="445">[</emphasis>
95 95 41.1 72 72 
<emphasis id="B925DC6DDB1AFFBA8E99FDBBFE5FFD8D" bold="true" box="[403,426,536,596]" pageId="5" pageNumber="445">Temp</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04FD34FD98FD76" blockId="5.[270,621,226,2111]" box="[270,621,648,738]" pageId="5" pageNumber="445">
. species 
<emphasis id="B925DC6DDB1AFFBA8E99FD2BFE5FFD12" bold="true" box="[403,426,648,715]" pageId="5" pageNumber="445">Cycles</emphasis>
1 35 35 35 1
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04FD49FE4DFCD5" blockId="5.[270,621,226,2111]" box="[270,440,746,786]" pageId="5" pageNumber="445">
new
<emphasis id="B925DC6DDB1AFFBA8EABFCA5FE4DFCD5" bold="true" box="[417,440,774,780]" pageId="5" pageNumber="445">]</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04FCB9FE4DFCBD" blockId="5.[270,621,226,2111]" pageId="5" pageNumber="445">
, 
<taxonomicName id="4C517BFCDB1AFFBA8E04FC83FED0FCBD" authorityName="Mapalo &amp; Stec &amp; Mirano-Bascos &amp; Michalczyk" authorityYear="2017" box="[270,293,800,868]" class="Eutardigrada" family="Macrobiotidae" genus="Mesobiotus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Parachela" pageId="5" pageNumber="445" phylum="Tardigrada" rank="species" species="insanis">
<emphasis id="B925DC6DDB1AFFBA8E04FC83FED0FCBD" box="[270,293,800,868]" italics="true" pageId="5" pageNumber="445">insanis</emphasis>
</taxonomicName>
<emphasis id="B925DC6DDB1AFFBA8E5CFC87FE4DFCF4" bold="true" box="[342,440,780,862]" pageId="5" pageNumber="445">ITS- 2 Time: min sec</emphasis>
: 00 10 00: 30: 00 30 01 00: 10 00: 
<emphasis id="B925DC6DDB1AFFBA8EABFCFDFE4DFCBD" bold="true" box="[417,440,862,868]" pageId="5" pageNumber="445">[</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04FCCFFE5FFC7F" blockId="5.[270,621,226,2111]" box="[270,426,876,987]" pageId="5" pageNumber="445">
<taxonomicName id="4C517BFCDB1AFFBA8E04FCCFFED0FC02" authorityName="Vecchi, Cesari, Bertolani, Jonsson, Rebecchi &amp; Guidetti" authorityYear="2016" box="[270,293,876,987]" class="Eutardigrada" family="Macrobiotidae" genus="Mesobiotus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Parachela" pageId="5" pageNumber="445" phylum="Tardigrada" rank="genus">
<emphasis id="B925DC6DDB1AFFBA8E04FCCFFED0FC02" box="[270,293,876,987]" italics="true" pageId="5" pageNumber="445">Mesobiotus</emphasis>
</taxonomicName>
<emphasis id="B925DC6DDB1AFFBA8E99FC0DFE5FFC7F" bold="true" box="[403,426,914,949]" pageId="5" pageNumber="445">[C</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E99FC28FE5FFC76" blockId="5.[270,621,226,2111]" box="[403,426,907,943]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E99FC28FE5FFC76" bold="true" box="[403,426,907,943]" pageId="5" pageNumber="445">] °</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04FC41FED0FC2F" blockId="5.[270,621,226,2111]" box="[270,293,994,1014]" pageId="5" pageNumber="445">of</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E99FC1EFED0FB4A" blockId="5.[270,621,226,2111]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E99FC1EFE5FFC20" bold="true" box="[403,426,957,1017]" pageId="5" pageNumber="445">Temp</emphasis>
95 95 50 72 72 fragments 
<emphasis id="B925DC6DDB1AFFBA8E99FB8EFE5FFBA9" bold="true" box="[403,426,1069,1136]" pageId="5" pageNumber="445">Cycles</emphasis>
1 35 35 35 1 gene
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04FB38FED0FB36" blockId="5.[270,621,226,2111]" box="[270,293,1179,1263]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E04FB38FED0FB1D" box="[270,293,1179,1220]" italics="true" pageId="5" pageNumber="445">COI</emphasis>
and
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04FB5EFED0FAD1" blockId="5.[270,621,226,2111]" box="[270,293,1277,1288]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E04FB5EFED0FAD1" box="[270,293,1277,1288]" italics="true" pageId="5" pageNumber="445">2</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04FB54FED0FB24" blockId="5.[270,621,226,2111]" box="[270,293,1271,1277]" pageId="5" pageNumber="445">,</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E5CFB48FD98FB03" blockId="5.[270,621,226,2111]" box="[342,621,1189,1284]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E5CFB48FE4DFB0A" bold="true" box="[342,440,1189,1284]" pageId="5" pageNumber="445">S rRNA Time: min] sec</emphasis>
: 10 00: 00 30: 30 00 01 00: 10: 00
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E5CFB5BFE4DFAD3" blockId="5.[270,621,226,2111]" box="[342,440,1272,1297]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E5CFB5BFE4DFAD3" bold="true" box="[342,440,1272,1297]" pageId="5" pageNumber="445">28 [</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04FAABFED0FAEB" blockId="5.[270,621,226,2111]" box="[270,293,1288,1330]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E04FAABFED0FAEB" box="[270,293,1288,1330]" italics="true" pageId="5" pageNumber="445">ITS-</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E99FA93FE5FFAEE" blockId="5.[270,621,226,2111]" box="[403,426,1328,1335]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E99FA93FE5FFAEE" bold="true" box="[403,426,1328,1335]" pageId="5" pageNumber="445">]</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04FA99FED0FA99" blockId="5.[270,621,226,2111]" box="[270,293,1338,1344]" pageId="5" pageNumber="445">,</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E99FA94FE5FFA92" blockId="5.[270,621,226,2111]" box="[403,426,1335,1355]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E99FA94FE5FFA92" bold="true" box="[403,426,1335,1355]" pageId="5" pageNumber="445">C</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04FAE3FE5FFA8D" blockId="5.[270,621,226,2111]" box="[270,426,1344,1399]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E04FAE3FED0FAAE" box="[270,293,1344,1399]" italics="true" pageId="5" pageNumber="445">rRNA</emphasis>
<emphasis id="B925DC6DDB1AFFBA8E99FAF7FE5FFA8D" bold="true" box="[403,426,1355,1371]" pageId="5" pageNumber="445">[°</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04FADDFD98FAAA" blockId="5.[270,621,226,2111]" box="[270,621,1371,1442]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E04FADDFED0FA7B" box="[270,293,1406,1442]" italics="true" pageId="5" pageNumber="445">S 28</emphasis>
<emphasis id="B925DC6DDB1AFFBA8E99FAC1FE5FFA47" bold="true" box="[403,426,1378,1438]" pageId="5" pageNumber="445">Temp</emphasis>
95 95 50 72 72
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04FA09FED0FA69" blockId="5.[270,621,226,2111]" box="[270,293,1450,1456]" pageId="5" pageNumber="445">,</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04FA13FED0FA3E" blockId="5.[270,621,226,2111]" box="[270,293,1456,1511]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E04FA13FED0FA3E" box="[270,293,1456,1511]" italics="true" pageId="5" pageNumber="445">rRNA</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04FA4DFD98FA23" blockId="5.[270,621,226,2111]" box="[270,621,1490,1557]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8E04FA4DFED0F9CA" box="[270,293,1518,1555]" italics="true" pageId="5" pageNumber="445">S 18</emphasis>
<emphasis id="B925DC6DDB1AFFBA8E99FA71FE5FF9CC" bold="true" box="[403,426,1490,1557]" pageId="5" pageNumber="445">Cycles</emphasis>
1 35 35 35 1
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04F9B9FE4DF9A4" blockId="5.[270,621,226,2111]" box="[270,440,1562,1717]" pageId="5" pageNumber="445">
of amplification 
<emphasis id="B925DC6DDB1AFFBA8E5CF9E9FE4DF9A4" bold="true" box="[342,440,1610,1717]" pageId="5" pageNumber="445">rRNA 18S Time sec min:</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8EABF9F2FE5FF905" blockId="5.[270,621,226,2111]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8EABF9F2FE4DF976" bold="true" box="[417,440,1617,1711]" pageId="5" pageNumber="445">] [</emphasis>
: 10 00:30 00 30 00: 01: 00 00 10: the
<emphasis id="B925DC6DDB1AFFBA8E99F976FE5FF905" bold="true" box="[403,426,1749,1756]" pageId="5" pageNumber="445">]</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04F942FE5FF8D9" blockId="5.[270,621,226,2111]" box="[270,426,1757,1792]" pageId="5" pageNumber="445">
for 
<emphasis id="B925DC6DDB1AFFBA8E99F953FE5FF8D9" bold="true" box="[403,426,1757,1792]" pageId="5" pageNumber="445">°C [</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04F8A6FED0F868" blockId="5.[270,621,226,2111]" pageId="5" pageNumber="445">
used 
<emphasis id="B925DC6DDB1AFFBA8E99F8ABFE5FF89D" bold="true" box="[403,426,1800,1860]" pageId="5" pageNumber="445">Temp</emphasis>
95 95 55 72 72 programmes
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04F81AFED0F826" blockId="5.[270,621,226,2111]" pageId="5" pageNumber="445">PCR. denaturation Denaturation elongation 2</paragraph>
<paragraph id="8BEE007FDB1AFFBA8E04F7ABFE4DF7F6" blockId="5.[270,621,226,2111]" box="[270,440,2050,2111]" pageId="5" pageNumber="445">
Table 
<emphasis id="B925DC6DDB1AFFBA8EABF7A1FE4DF7F6" bold="true" box="[417,440,2050,2095]" pageId="5" pageNumber="445">Step</emphasis>
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8EDDF855FBFCFEAC" blockId="5.[270,621,226,2111]" lastBlockId="5.[835,1474,189,374]" pageId="5" pageNumber="445">
Initial Annealing Elongation Final wrinkles (
<figureCitation id="136A1CFADB1AFFBA8CBEFF1EFBCDFF0C" box="[948,1080,189,213]" captionStart="Fig" captionStartId="10.[113,148,2036,2058]" captionTargetBox="[134,1430,189,2020]" captionTargetId="figure-11@10.[134,1430,189,2020]" captionTargetPageId="10" captionText="Fig. 7. Mesobiotus insanis, new species, magnified PCM images of the surface of the eggs shown in Fig. 4 (respective letters indicate same eggs in both figures). Filled arrowheads indicate the whorled sculpturing inside areolae; empty arrowheads indicate the bubble-like structures within the rims delimiting the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357628" httpUri="https://zenodo.org/record/5357628/files/figure.png" pageId="5" pageNumber="445">Figs. 7A–C</figureCitation>
, 
<figureCitation id="136A1CFADB1AFFBA8B4DFF1EFB70FF0C" box="[1095,1157,189,213]" captionStart="Fig" captionStartId="11.[135,170,1987,2009]" captionTargetBox="[136,1474,237,1971]" captionTargetId="figure-72@11.[136,1474,237,1971]" captionTargetPageId="11" captionText="Fig. 8. Mesobiotus insanis, new species, SEM images of eggs. A, B, entire view of two different eggs; C, D, egg surface with processes; E, egg process; F, egg surface between processes, with evident pores and whorl-shaped sculpturing. Arrows indicate pores in the processes, flat arrowheads indicate the whorl-shaped surface inside the areolae, indented arrowheads indicate pores inside the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357630" httpUri="https://zenodo.org/record/5357630/files/figure.png" pageId="5" pageNumber="445">8C–F</figureCitation>
) and porous surface around the whorl (
<figureCitation id="136A1CFADB1AFFBA8CB4FF7EFBC0FF2C" box="[958,1077,221,245]" captionStart="Fig" captionStartId="11.[135,170,1987,2009]" captionTargetBox="[136,1474,237,1971]" captionTargetId="figure-72@11.[136,1474,237,1971]" captionTargetPageId="11" captionText="Fig. 8. Mesobiotus insanis, new species, SEM images of eggs. A, B, entire view of two different eggs; C, D, egg surface with processes; E, egg process; F, egg surface between processes, with evident pores and whorl-shaped sculpturing. Arrows indicate pores in the processes, flat arrowheads indicate the whorl-shaped surface inside the areolae, indented arrowheads indicate pores inside the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357630" httpUri="https://zenodo.org/record/5357630/files/figure.png" pageId="5" pageNumber="445">Fig. 8D, F</figureCitation>
). Both whorls and pores are always clearly visible in SEM (
<figureCitation id="136A1CFADB1AFFBA8B5AFF5EFB33FECC" box="[1104,1222,253,277]" captionStart="Fig" captionStartId="11.[135,170,1987,2009]" captionTargetBox="[136,1474,237,1971]" captionTargetId="figure-72@11.[136,1474,237,1971]" captionTargetPageId="11" captionText="Fig. 8. Mesobiotus insanis, new species, SEM images of eggs. A, B, entire view of two different eggs; C, D, egg surface with processes; E, egg process; F, egg surface between processes, with evident pores and whorl-shaped sculpturing. Arrows indicate pores in the processes, flat arrowheads indicate the whorl-shaped surface inside the areolae, indented arrowheads indicate pores inside the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357630" httpUri="https://zenodo.org/record/5357630/files/figure.png" pageId="5" pageNumber="445">Fig. 8C–F</figureCitation>
), however under PCM pores are never identifiable (they are below the resolution of light microscope) whereas whorls are visible only in some eggs (
<figureCitation id="136A1CFADB1AFFBA8C8FFEFEFC08FEAC" box="[901,1021,349,373]" captionStart="Fig" captionStartId="10.[113,148,2036,2058]" captionTargetBox="[134,1430,189,2020]" captionTargetId="figure-11@10.[134,1430,189,2020]" captionTargetPageId="10" captionText="Fig. 7. Mesobiotus insanis, new species, magnified PCM images of the surface of the eggs shown in Fig. 4 (respective letters indicate same eggs in both figures). Filled arrowheads indicate the whorled sculpturing inside areolae; empty arrowheads indicate the bubble-like structures within the rims delimiting the areolae. Scale bars in μm." figureDoi="http://doi.org/10.5281/zenodo.5357628" httpUri="https://zenodo.org/record/5357628/files/figure.png" pageId="5" pageNumber="445">Fig. 7A–C</figureCitation>
).
</paragraph>
</subSubSection>
<subSubSection id="C34B53F4DB1AFFBA8C49FE3EFBF5FAEC" pageId="5" pageNumber="445" type="discussion">
<paragraph id="8BEE007FDB1AFFBA8C49FE3EFA98FBEC" blockId="5.[835,1475,413,1077]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8C49FE3EFC40FE6C" bold="true" box="[835,949,413,437]" pageId="5" pageNumber="445">Remarks.</emphasis>
Eggs of the new species exhibit considerable variation in chorion morphology (
<figureCitation id="136A1CFADB1AFFBA8BA5FE1EFAEDFE0C" box="[1199,1304,445,469]" captionStart-0="Fig" captionStart-1="Fig" captionStart-2="Fig" captionStart-3="Fig" captionStart-4="Fig" captionStartId-0="6.[113,148,2093,2115]" captionStartId-1="7.[135,170,2064,2086]" captionStartId-2="9.[136,171,1673,1695]" captionStartId-3="10.[113,148,2036,2058]" captionStartId-4="11.[135,170,1987,2009]" captionTargetBox-0="[114,1451,189,2077]" captionTargetBox-1="[146,1462,189,2049]" captionTargetBox-2="[135,1474,664,1657]" captionTargetBox-3="[134,1430,189,2020]" captionTargetBox-4="[136,1474,237,1971]" captionTargetId-0="figure-10@6.[114,1451,189,2077]" captionTargetId-1="figure-7@7.[146,1462,189,2049]" captionTargetId-2="figure-359@9.[135,1474,664,1657]" captionTargetId-3="figure-11@10.[134,1430,189,2020]" captionTargetId-4="figure-72@11.[136,1474,237,1971]" captionTargetPageId-0="6" captionTargetPageId-1="7" captionTargetPageId-2="9" captionTargetPageId-3="10" captionTargetPageId-4="11" captionText-0="Fig. 4. Mesobiotus insanis, new species, PCM images of midsections of six different eggs. Scale bars in μm." captionText-1="Fig. 5. Mesobiotus insanis, new species, PCM images of the surfaces of the eggs shown in Fig. 4 (respective letters indicate same eggs in both figures). Scale bars in μm." captionText-2="Fig. 6. Mesobiotus insanis, new species, PCM images of the midsection of processes from various eggs. Scale bars in μm." captionText-3="Fig. 7. Mesobiotus insanis, new species, magnified PCM images of the surface of the eggs shown in Fig. 4 (respective letters indicate same eggs in both figures). Filled arrowheads indicate the whorled sculpturing inside areolae; empty arrowheads indicate the bubble-like structures within the rims delimiting the areolae. Scale bars in μm." captionText-4="Fig. 8. Mesobiotus insanis, new species, SEM images of eggs. A, B, entire view of two different eggs; C, D, egg surface with processes; E, egg process; F, egg surface between processes, with evident pores and whorl-shaped sculpturing. Arrows indicate pores in the processes, flat arrowheads indicate the whorl-shaped surface inside the areolae, indented arrowheads indicate pores inside the areolae. Scale bars in μm." figureDoi-0="http://doi.org/10.5281/zenodo.5357622" figureDoi-1="http://doi.org/10.5281/zenodo.5357624" figureDoi-2="http://doi.org/10.5281/zenodo.5357626" figureDoi-3="http://doi.org/10.5281/zenodo.5357628" figureDoi-4="http://doi.org/10.5281/zenodo.5357630" httpUri-0="https://zenodo.org/record/5357622/files/figure.png" httpUri-1="https://zenodo.org/record/5357624/files/figure.png" httpUri-2="https://zenodo.org/record/5357626/files/figure.png" httpUri-3="https://zenodo.org/record/5357628/files/figure.png" httpUri-4="https://zenodo.org/record/5357630/files/figure.png" pageId="5" pageNumber="445">Figs. 4–8</figureCitation>
). Given that the eggs were obtained in culture, it should be considered whether similar variability could be observed in the wild. The great majority of eutardigrade species descriptions provide images of only most typical eggs morphotypes, which may result in a conviction that eutardigrade eggs are characterised by low intra-specific variability. However, recent studies show that at least in some eutardigrade species, egg chorion may exhibit considerable morphological and morphometric variation in natural populations (
<bibRefCitation id="EFC07D8EDB1AFFBA8B21FD7EFB1FFD2C" author="Stec D &amp; Zawierucha K &amp; Michalczyk L" box="[1067,1258,733,757]" pageId="5" pageNumber="445" pagination="403 - 420" refId="ref9033" refString="Stec D, Zawierucha K &amp; Michalczyk L (2017) An integrative description of Ramazzottius subanomalus (Biserov, 1985) (Tardigrada) from Poland. Zootaxa, 4300 (3): 403 - 420. https: // dx. doi. org / 10.11646 / zootaxa. 4300.3.4." type="journal article" year="2017">Stec et al., 2017</bibRefCitation>
; 
<bibRefCitation id="EFC07D8EDB1AFFBA8BF2FD7EFC8BFCCC" author="Zawierucha K &amp; Kolicka M &amp; Kaczmarek L" pageId="5" pageNumber="445" pagination="498 - 510" refId="ref9341" refString="Zawierucha K, Kolicka M &amp; Kaczmarek L (2016) Re-description of the Arctic tardigrade Tenuibiotus voronkovi (Tumanov, 2007) (Eutardigrades; Macrobiotidae), with the first molecular data for the genus. Zootaxa, 4196 (4): 498 - 510. http: // dx. doi. org / 10.11646 / zootaxa. 4196.4.2." type="journal article" year="2016">Zawierucha et al., 2016</bibRefCitation>
). Moreover, eggs of 
<emphasis id="B925DC6DDB1AFFBA8B57FD5DFAFDFCCC" box="[1117,1288,765,789]" italics="true" pageId="5" pageNumber="445">R. subanomalus</emphasis>
obtained by 
<bibRefCitation id="EFC07D8EDB1AFFBA8A9EFD5EFC25FCEC" author="Stec D &amp; Morek W &amp; Gasiorek P &amp; Kaczmarek L &amp; Michalczyk L" pageId="5" pageNumber="445" pagination="176 - 188" refId="ref8906" refString="Stec D, Morek W, Gasiorek P, Kaczmarek L &amp; Michalczyk L (2016 b) Determinants and taxonomic consequences of extreme egg shell variability in Ramazzottius subanomalus (Biserov, 1985) (Tardigrada). Zootaxa, 4208 (2): 176 - 188. https: // dx. doi. org / 10.11646 / zootaxa. 4208.2.5." type="journal article" year="2016">Stec et al. (2016b)</bibRefCitation>
in a laboratory culture showed the same extent of variation as eggs of the same species extracted from a moss sample by 
<bibRefCitation id="EFC07D8EDB1AFFBA8CF1FCFEFB4FFCAC" author="Stec D &amp; Zawierucha K &amp; Michalczyk L" box="[1019,1210,861,885]" pageId="5" pageNumber="445" pagination="403 - 420" refId="ref9033" refString="Stec D, Zawierucha K &amp; Michalczyk L (2017) An integrative description of Ramazzottius subanomalus (Biserov, 1985) (Tardigrada) from Poland. Zootaxa, 4300 (3): 403 - 420. https: // dx. doi. org / 10.11646 / zootaxa. 4300.3.4." type="journal article" year="2017">Stec et al. (2017)</bibRefCitation>
, which suggests that 
<taxonomicName id="4C517BFCDB1AFFBA8AAFFCFDFC7AFC4C" authority="Mapalo &amp; Stec &amp; Mirano-Bascos &amp; Michalczyk, 2017" authorityName="Mapalo &amp; Stec &amp; Mirano-Bascos &amp; Michalczyk" authorityYear="2017" class="Eutardigrada" family="Macrobiotidae" genus="Mesobiotus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Parachela" pageId="5" pageNumber="445" phylum="Tardigrada" rank="species" species="insanis" status="sp. nov.">
<emphasis id="B925DC6DDB1AFFBA8AAFFCFDFC7AFC4C" italics="true" pageId="5" pageNumber="445">M. insanis</emphasis>
</taxonomicName>
, 
<taxonomicNameLabel id="A2166116DB1AFFBA8C94FCDDFBD6FC4C" box="[926,1059,893,917]" pageId="5" pageNumber="445" rank="species">new species</taxonomicNameLabel>
, also may lay variable eggs in nature. Nevertheless, even if laboratory conditions indeed increased egg shell variability, the narrower natural variation would fall within that observed in the in vitro culture. In other words, a greater variability presented in the description would allow an easier identification of the species in the future.
</paragraph>
<paragraph id="8BEE007FDB1AFFBA8C49FBFEFBF5FAEC" blockId="5.[835,1475,1117,1333]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8C49FBFEFBF5FBAC" bold="true" box="[835,1024,1117,1141]" pageId="5" pageNumber="445">DNA Sequences.</emphasis>
A single haplotype was found for each of the four sequenced markers in all seven analysed individuals representing the three subcultures. The sequences were deposited in GenBank with the following reference numbers: 
<emphasis id="B925DC6DDB1AFFBA8C49FB7EFC5BFB2C" box="[835,942,1245,1269]" italics="true" pageId="5" pageNumber="445">18S rRNA</emphasis>
, 499 bp long, 
<accessionNumber id="94029D9CDB1AFFBA8B4EFB7EFB48FB2C" box="[1092,1213,1245,1269]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/MF441488" pageId="5" pageNumber="445">MF441488</accessionNumber>
; 
<emphasis id="B925DC6DDB1AFFBA8BCDFB7EFAC7FB2C" box="[1223,1330,1245,1269]" italics="true" pageId="5" pageNumber="445">28S rRNA</emphasis>
, 769 bp long, 
<accessionNumber id="94029D9CDB1AFFBA8C49FB5EFC4BFACC" box="[835,958,1277,1301]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/MF441489" pageId="5" pageNumber="445">MF441489</accessionNumber>
; 
<emphasis id="B925DC6DDB1AFFBA8CC1FB5DFC05FACC" box="[971,1008,1278,1301]" italics="true" pageId="5" pageNumber="445">ITS</emphasis>
-2, 352 bp long, 
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; 
<emphasis id="B925DC6DDB1AFFBA8A3DFB5DFA90FACC" box="[1335,1381,1278,1301]" italics="true" pageId="5" pageNumber="445">COI</emphasis>
, 771 bp long, 
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.
</paragraph>
</subSubSection>
<subSubSection id="C34B53F4DB1AFFBA8C49FAFEFB6CFA6C" pageId="5" pageNumber="445" type="etymology">
<paragraph id="8BEE007FDB1AFFBA8C49FAFEFB6CFA6C" blockId="5.[835,1475,1373,1462]" pageId="5" pageNumber="445">
<emphasis id="B925DC6DDB1AFFBA8C49FAFEFC39FAAC" bold="true" box="[835,972,1373,1397]" pageId="5" pageNumber="445">Etymology.</emphasis>
The name of the new species refers to its insanely complex egg morphology, never observed before in any other tardigrade species.
</paragraph>
</subSubSection>
</treatment>
</document>