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<document id="66ADEF742779A237CD35532F8C3E779C" ID-CLB-Dataset="21967" ID-DOI="10.5852/ejt.2016.196" ID-GBIF-Dataset="0d3456bf-150b-4cd2-ae80-e050a4852458" ID-ISSN="2118-9773" ID-Zenodo-Dep="3837952" IM.bibliography_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1590071350476" checkinUser="valdenar" docAuthor="Garić, Rade &amp; Batistić, Mirna" docDate="2016" docId="03A287CDFFAFFF98185FE4FFFCCAFCE6" docLanguage="en" docName="ejt-2016-196.pdf.imf" docOrigin="European Journal of Taxonomy 196" docStyle="DocumentStyle:EF2B578F1D15862ADE45B0C07C620911.14:EJT.2018-.journal_article.type1" docStyleId="EF2B578F1D15862ADE45B0C07C620911" docStyleName="EJT.2018-.journal_article.type1" docStyleVersion="14" docTitle="Brooksia lacromae Garić &amp; Batistić 2016, sp. nov." docType="treatment" docUuid="2B9ACCBD-8B20-437D-B013-468F8E956864" docUuidSource="ZooBank" docVersion="9" lastPageNumber="10" masterDocId="FF9BFFB5FFABFF911A29E254FFA8FFDA" masterDocTitle="Description of Brooksia lacromae sp. nov. (Tunicata, Thaliacea) from the Adriatic Sea" masterLastPageNumber="13" masterPageNumber="1" pageNumber="5" updateTime="1698840620082" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-3.0" zenodo-license-figures="CC-BY-3.0" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="8CDBC52E25EFD3F641900F43DFAB7ADB">Description of Brooksia lacromae sp. nov. (Tunicata, Thaliacea) from the Adriatic Sea</mods:title>
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<mods:namePart id="2A14FE34A3BE86F0B93F53F7B9478FA0">Garić, Rade</mods:namePart>
<mods:affiliation id="24D632F095B3E8052CEB98604A6AC23A">Institute for Marine and Coastal Research, University of Dubrovnik, Kneza Damjana Jude 12, 20000 Dubrovnik, Croatia. &amp; Email: rade. garic @ unidu. hr</mods:affiliation>
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<mods:namePart id="2006BF7C33AE8745DF537EAB359A842E">Batistić, Mirna</mods:namePart>
<mods:affiliation id="042E101C46633D531076734CAD525242">Institute for Marine and Coastal Research, University of Dubrovnik, Kneza Damjana Jude 12, 20000 Dubrovnik, Croatia. &amp; Corresponding author: mirna. batistic @ unidu. hr</mods:affiliation>
<mods:nameIdentifier id="204AC3F72C7C2249F50FD055EB3500D0" type="email">mirna.batistic@unidu.hr</mods:nameIdentifier>
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<mods:title id="B6DC14E08873999FAAEB2B45DEC15D9C">European Journal of Taxonomy</mods:title>
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<mods:date id="C1C39C1B4AE9AB872DF35DDB929C78BF">2016</mods:date>
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<treatment id="03A287CDFFAFFF98185FE4FFFCCAFCE6" ID-DOI="http://doi.org/10.5281/zenodo.3852158" ID-GBIF-Taxon="164246079" ID-Zenodo-Dep="3852158" LSID="urn:lsid:zoobank.org:act:2B9ACCBD-8B20-437D-B013-468F8E956864" httpUri="http://treatment.plazi.org/id/03A287CDFFAFFF98185FE4FFFCCAFCE6" lastPageId="9" lastPageNumber="10" pageId="4" pageNumber="5">
<subSubSection id="C3116550FFAFFF95185FE4FFFB6EF933" pageId="4" pageNumber="5" type="nomenclature">
<paragraph id="8BB436DBFFAFFF95185FE4FFFC15F91C" blockId="4.[366,1222,1707,1804]" box="[630,957,1707,1734]" pageId="4" pageNumber="5">
<heading id="D0FC81B7FFAFFF95185FE4FFFC15F91C" box="[630,957,1707,1734]" centered="true" fontSize="11" level="2" pageId="4" pageNumber="5" reason="2">
<taxonomicName id="4C0B4D58FFAFFF95185FE4FFFCF3F91C" ID-CoL="NDT7" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[630,859,1707,1734]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="4" pageNumber="5" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFAFFF95185FE4FFFCF3F91C" bold="true" box="[630,859,1707,1734]" italics="true" pageId="4" pageNumber="5">Brooksia lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFAFFF95194AE4F8FC15F91C" box="[867,957,1708,1734]" pageId="4" pageNumber="5" rank="species">sp. nov.</taxonomicNameLabel>
</heading>
</paragraph>
<paragraph id="8BB436DBFFAFFF951B47E49AFB6EF933" blockId="4.[366,1222,1707,1804]" box="[366,1222,1742,1769]" pageId="4" pageNumber="5">
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urn:lsid:zoobank.org:act:
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</uri>
</paragraph>
</subSubSection>
<subSubSection id="C3116550FFAFFF9518CEE4A6FCE4F8D6" box="[743,844,1778,1804]" pageId="4" pageNumber="5" type="description">
<paragraph id="8BB436DBFFAFFF9518CEE4A6FCE4F8D6" blockId="4.[366,1222,1707,1804]" box="[743,844,1778,1804]" pageId="4" pageNumber="5">
<figureCitation id="13302A5EFFAFFF9518CEE4A6FCE4F8D6" box="[743,844,1778,1804]" captionStart-0="Fig" captionStart-1="Fig" captionStart-2="Fig" captionStart-3="Fig" captionStartId-0="1.[189,232,1964,1990]" captionStartId-1="5.[189,232,1789,1815]" captionStartId-2="6.[189,232,1858,1884]" captionStartId-3="7.[189,231,1894,1920]" captionTargetBox-0="[193,1400,1389,1912]" captionTargetBox-1="[235,1328,407,1719]" captionTargetBox-2="[255,1339,1128,1801]" captionTargetBox-3="[275,1315,802,1848]" captionTargetId-0="figure@1.[187,1409,1376,1923]" captionTargetId-1="figure@5.[219,1353,390,1747]" captionTargetId-2="figure@6.[223,1357,1090,1817]" captionTargetId-3="figure@7.[257,1315,790,1858]" captionTargetPageId-0="1" captionTargetPageId-1="5" captionTargetPageId-2="6" captionTargetPageId-3="7" captionText-0="Fig. 1. Position of the Lokrum station in the South Adriatic, where specimens of Brooksia lacromae sp. nov. were collected." captionText-1="Fig. 2. Oozooid of Brooksia lacromae sp. nov. A. Dorsal view, drawing. B. Ventral view, drawing. C. Dorsal view, photograph of an individual dyed with Janus Green, with an arrow indicating breakage in the proboscis during collection. Abbreviations: as = atrial siphon; bl = branching of the ventral longitudinal muscle; br = branchial bar; dt = dorsal tubercle; en = endostyle; g = ganglion; im = intermediate muscle; lm = longitudinal muscle; lo = openings in the longitudinal muscle; nu = nucleus; os = oral siphon; p = proboscis; pb = peripharyngeal band; st = stolon; tp = test processus; IVII = body muscles." captionText-2="Fig. 3. Drawing of the blastozooid of Brooksia lacromae sp. nov. (sinistral individual). A. Dorsal view. B. Ventral view. Abbreviations: ap13 = attachment processes; as = atrial siphon; br = branchial bar; dt = dorsal tubercle; e = embryo; en = endostyle; g = ganglion; h = heart; nu = nucleus; os = oral siphon; pb = peripharyngeal band; ILIVL = left side muscles; IRIIIR = right side muscles; IM1 and IM2 = intermediate muscles." captionText-3="Fig. 4. Brooksia lacromae sp. nov. A. Photograph of the ventral side of the dextral blastozooid. B. Photograph of the dorsal side of the sinistral blastozooid. C. Photograph of the lateral side of a blastozooid. D. Detail of the ventral posterior end of an oozooid. All indivuduals were dyed with Janus Green dye. Abbreviations: st = stolon; tp = test processus." figureDoi-0="http://doi.org/10.5281/zenodo.3837954" figureDoi-1="http://doi.org/10.5281/zenodo.3837956" figureDoi-2="http://doi.org/10.5281/zenodo.3837958" figureDoi-3="http://doi.org/10.5281/zenodo.3837960" httpUri-0="https://zenodo.org/record/3837954/files/figure.png" httpUri-1="https://zenodo.org/record/3837956/files/figure.png" httpUri-2="https://zenodo.org/record/3837958/files/figure.png" httpUri-3="https://zenodo.org/record/3837960/files/figure.png" pageId="4" pageNumber="5">Figs 14</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="C3116550FFAFFF961A94E567FDCCFE92" lastPageId="7" lastPageNumber="8" pageId="4" pageNumber="5" type="diagnosis">
<paragraph id="8BB436DBFFAFFF951A94E567FE9EF897" blockId="4.[189,310,1843,1869]" box="[189,310,1843,1869]" pageId="4" pageNumber="5">
<heading id="D0FC81B7FFAFFF951A94E567FE9EF897" bold="true" box="[189,310,1843,1869]" fontSize="11" level="3" pageId="4" pageNumber="5" reason="3">
<emphasis id="B97FEAC9FFAFFF951A94E567FE9EF897" bold="true" box="[189,310,1843,1869]" pageId="4" pageNumber="5">Diagnosis</emphasis>
</heading>
</paragraph>
<paragraph id="8BB436DBFFAFFF951AC3E536FDF2F8A6" blockId="4.[189,1399,1890,2022]" box="[234,602,1890,1916]" pageId="4" pageNumber="5">
<emphasis id="B97FEAC9FFAFFF951AC3E536FEFAF8A6" bold="true" box="[234,338,1890,1916]" pageId="4" pageNumber="5">Oozooid</emphasis>
(solitary form;
<figureCitation id="13302A5EFFAFFF951825E536FDF9F8A6" box="[524,593,1890,1916]" captionStart="Fig" captionStartId="5.[189,232,1789,1815]" captionTargetBox="[235,1328,407,1719]" captionTargetId="figure@5.[219,1353,390,1747]" captionTargetPageId="5" captionText="Fig. 2. Oozooid of Brooksia lacromae sp. nov. A. Dorsal view, drawing. B. Ventral view, drawing. C. Dorsal view, photograph of an individual dyed with Janus Green, with an arrow indicating breakage in the proboscis during collection. Abbreviations: as = atrial siphon; bl = branching of the ventral longitudinal muscle; br = branchial bar; dt = dorsal tubercle; en = endostyle; g = ganglion; im = intermediate muscle; lm = longitudinal muscle; lo = openings in the longitudinal muscle; nu = nucleus; os = oral siphon; p = proboscis; pb = peripharyngeal band; st = stolon; tp = test processus; IVII = body muscles." figureDoi="http://doi.org/10.5281/zenodo.3837956" httpUri="https://zenodo.org/record/3837956/files/figure.png" pageId="4" pageNumber="5">Fig. 2</figureCitation>
)
</paragraph>
<paragraph id="8BB436DBFFAFFF971A94E5D1FEEFFE22" blockId="4.[189,1399,1890,2022]" lastBlockId="6.[189,1399,267,504]" lastPageId="6" lastPageNumber="7" pageId="4" pageNumber="5">
Muscles II and III fuse dorsally before fusing with muscle I mid-dorsally. Muscles III and IV fuse briefly dorsolaterally. Muscles IV and V fuse before approaching muscles VI and VII mid-dorsally. There is one longitudinal ventral muscle which extends anteriorly into the proboscis. In addition, the proboscis contains two lateral longitudinal muscles. The ventral longitudinal muscle branches posteriorly into two branches. There are two slit-like openings in the ventral longitudinal muscle, one below the anterior end of the endostyle, and the other slightly anteriorly from the first one. Muscle I fuses with branches of the ventral longitudinal muscle at their anterior part, while muscles VI and VII fuse with them posteriorly. Muscles II, III, IV and V converge ventrally towards the junction between muscle I and the branches of the ventral longitudinal muscle. Muscle II can be slightly fused with muscle I just before its connection with branches of the ventral longitudinal muscle. Endostyle straight. Stolon emerges from the test ventrally, slightly posteriorly from the nucleus. There is one test processus just posteriorly from the stolon (
<figureCitation id="13302A5EFFADFF971B3CE3EFFEDDFE0F" box="[277,373,443,469]" captionStart="Fig" captionStartId="7.[189,231,1894,1920]" captionTargetBox="[275,1315,802,1848]" captionTargetId="figure@7.[257,1315,790,1858]" captionTargetPageId="7" captionText="Fig. 4. Brooksia lacromae sp. nov. A. Photograph of the ventral side of the dextral blastozooid. B. Photograph of the dorsal side of the sinistral blastozooid. C. Photograph of the lateral side of a blastozooid. D. Detail of the ventral posterior end of an oozooid. All indivuduals were dyed with Janus Green dye. Abbreviations: st = stolon; tp = test processus." figureDoi="http://doi.org/10.5281/zenodo.3837960" httpUri="https://zenodo.org/record/3837960/files/figure.png" pageId="6" pageNumber="7">Fig. 4D</figureCitation>
). Muscles IVII with 5258 muscle fibers in total, measured laterally in two examined individuals.
</paragraph>
<caption id="DF746653FFAEFF941A94E4A9FE8BF831" ID-DOI="http://doi.org/10.5281/zenodo.3837956" ID-Zenodo-Dep="3837956" httpUri="https://zenodo.org/record/3837956/files/figure.png" pageId="5" pageNumber="6" startId="5.[189,232,1789,1815]" targetBox="[235,1328,407,1719]" targetPageId="5">
<paragraph id="8BB436DBFFAEFF941A94E4A9FE8BF831" blockId="5.[189,1399,1789,2027]" pageId="5" pageNumber="6">
<emphasis id="B97FEAC9FFAEFF941A94E4A9FEA6F8CD" bold="true" box="[189,270,1789,1815]" pageId="5" pageNumber="6">Fig. 2.</emphasis>
Oozooid of
<taxonomicName id="4C0B4D58FFAEFF941B84E4AAFD27F8C2" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[429,655,1790,1816]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="5" pageNumber="6" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFAEFF941B84E4AAFD27F8C2" box="[429,655,1790,1816]" italics="true" pageId="5" pageNumber="6">Brooksia lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFAEFF9418B3E4AAFD50F8C2" box="[666,760,1790,1816]" pageId="5" pageNumber="6" rank="species">sp. nov.</taxonomicNameLabel>
<emphasis id="B97FEAC9FFAEFF94192AE4A9FCB0F8CD" bold="true" box="[771,792,1789,1815]" pageId="5" pageNumber="6">A</emphasis>
. Dorsal view, drawing.
<emphasis id="B97FEAC9FFAEFF941E69E4AAFBFCF8C2" bold="true" box="[1088,1108,1790,1816]" pageId="5" pageNumber="6">B</emphasis>
. Ventral view, drawing.
<emphasis id="B97FEAC9FFAEFF941A94E575FF7AF8E1" bold="true" box="[189,210,1825,1851]" pageId="5" pageNumber="6">C</emphasis>
. Dorsal view, photograph of an individual dyed with Janus Green, with an arrow indicating breakage in the proboscis during collection. Abbreviations: as = atrial siphon; bl = branching of the ventral longitudinal muscle; br = branchial bar; dt = dorsal tubercle; en = endostyle; g = ganglion; im = intermediate muscle; lm = longitudinal muscle; lo = openings in the longitudinal muscle; nu = nucleus; os = oral siphon; p = proboscis; pb = peripharyngeal band; st = stolon; tp = test processus; IVII = body muscles.
</paragraph>
</caption>
<paragraph id="8BB436DBFFADFF971AC3E074FD5DFDE0" blockId="6.[189,1399,544,992]" box="[234,757,544,570]" pageId="6" pageNumber="7">
<emphasis id="B97FEAC9FFADFF971AC3E074FED3FDE0" bold="true" box="[234,379,544,570]" pageId="6" pageNumber="7">Blastozooid</emphasis>
(aggregate form;
<figureCitation id="13302A5EFFADFF971867E074FD30FDE0" box="[590,664,544,570]" captionStart="Fig" captionStartId="6.[189,232,1858,1884]" captionTargetBox="[255,1339,1128,1801]" captionTargetId="figure@6.[223,1357,1090,1817]" captionTargetPageId="6" captionText="Fig. 3. Drawing of the blastozooid of Brooksia lacromae sp. nov. (sinistral individual). A. Dorsal view. B. Ventral view. Abbreviations: ap13 = attachment processes; as = atrial siphon; br = branchial bar; dt = dorsal tubercle; e = embryo; en = endostyle; g = ganglion; h = heart; nu = nucleus; os = oral siphon; pb = peripharyngeal band; ILIVL = left side muscles; IRIIIR = right side muscles; IM1 and IM2 = intermediate muscles." figureDoi="http://doi.org/10.5281/zenodo.3837958" httpUri="https://zenodo.org/record/3837958/files/figure.png" pageId="6" pageNumber="7">Figs 3</figureCitation>
,
<figureCitation id="13302A5EFFADFF97188FE074FD1CFDE0" box="[678,692,544,570]" captionStart="Fig" captionStartId="7.[189,231,1894,1920]" captionTargetBox="[275,1315,802,1848]" captionTargetId="figure@7.[257,1315,790,1858]" captionTargetPageId="7" captionText="Fig. 4. Brooksia lacromae sp. nov. A. Photograph of the ventral side of the dextral blastozooid. B. Photograph of the dorsal side of the sinistral blastozooid. C. Photograph of the lateral side of a blastozooid. D. Detail of the ventral posterior end of an oozooid. All indivuduals were dyed with Janus Green dye. Abbreviations: st = stolon; tp = test processus." figureDoi="http://doi.org/10.5281/zenodo.3837960" httpUri="https://zenodo.org/record/3837960/files/figure.png" pageId="6" pageNumber="7">4</figureCitation>
AC)
</paragraph>
<paragraph id="8BB436DBFFADFF971A94E017FEAEFC3A" blockId="6.[189,1399,544,992]" pageId="6" pageNumber="7">Sinistral individual Four muscles on the left side, three muscles on the right side. Dorsal IM1 continues ventrally as the ventral muscle IM1, without extending to the anterior attachment processus (ap1). A branch of dorsal muscle IM1 (IM1-a) extends to the anterior attachment processus (ap1). Dorsal muscles IR, IIR and IIIR branch dichotomously. Dorsal muscle IR-a continues ventrally as ventral muscle IR. Dorsal muscle IR-b continues ventrally as muscle IIR-c. A small branch from muscle IR-b/IIR-c enters the lateral attachment processus (ap2). Dorsal muscle IIR-a continues ventrally as ventral muscle IIR-a. Muscle IIR-b ends blindly on the ventral side of the animal without entering the posterior attachment processus (ap3). Dorsal and ventral muscles IIIR enter the posterior attachment processus (ap3) without joining. Muscles IVL-a and IIIR-a extend from muscles IVL and IIIR, respectively, towards the nucleus. Endostyle curved anteriorly. Muscles IL-IIIL, IR-a,b and IIR-a,b,c with 3 muscle fibers each in all examined individuals. Muscle IVL with from 3 to 9 muscle fibers, muscle IIIR with from 5 to 7 muscle fibers.</paragraph>
<caption id="DF746653FFADFF971A94E516FE20F833" ID-DOI="http://doi.org/10.5281/zenodo.3837958" ID-Zenodo-Dep="3837958" httpUri="https://zenodo.org/record/3837958/files/figure.png" pageId="6" pageNumber="7" startId="6.[189,232,1858,1884]" targetBox="[255,1339,1128,1801]" targetPageId="6">
<paragraph id="8BB436DBFFADFF971A94E516FE20F833" blockId="6.[189,1399,1858,2025]" pageId="6" pageNumber="7">
<emphasis id="B97FEAC9FFADFF971A94E516FEA2F886" bold="true" box="[189,266,1858,1884]" pageId="6" pageNumber="7">Fig. 3.</emphasis>
Drawing of the blastozooid of
<taxonomicName id="4C0B4D58FFADFF971853E517FCF0F887" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[634,856,1859,1885]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="6" pageNumber="7" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFADFF971853E517FCF0F887" box="[634,856,1859,1885]" italics="true" pageId="6" pageNumber="7">Brooksia lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFADFF971976E517FC12F887" box="[863,954,1859,1885]" pageId="6" pageNumber="7" rank="species">sp. nov.</taxonomicNameLabel>
(sinistral individual).
<emphasis id="B97FEAC9FFADFF971E97E516FB7BF886" bold="true" box="[1214,1235,1858,1884]" pageId="6" pageNumber="7">A</emphasis>
. Dorsal view.
<emphasis id="B97FEAC9FFADFF971A94E532FF79F85A" bold="true" box="[189,209,1894,1920]" pageId="6" pageNumber="7">B</emphasis>
. Ventral view. Abbreviations: ap13 = attachment processes; as = atrial siphon; br = branchial bar; dt = dorsal tubercle; e = embryo; en = endostyle; g = ganglion; h = heart; nu = nucleus; os = oral siphon; pb = peripharyngeal band; I
<subScript id="178F349EFFADFF97182AE5EAFDA5F816" attach="left" box="[515,525,1982,1996]" fontSize="6" pageId="6" pageNumber="7">L</subScript>
IV
<subScript id="178F349EFFADFF971812E5EAFDEDF816" attach="left" box="[571,581,1982,1996]" fontSize="6" pageId="6" pageNumber="7">L</subScript>
= left side muscles; I
<subScript id="178F349EFFADFF971968E5EAFCE4F816" attach="left" box="[833,844,1982,1996]" fontSize="6" pageId="6" pageNumber="7">R</subScript>
III
<subScript id="178F349EFFADFF971951E5EAFC2BF816" attach="left" box="[888,899,1982,1996]" fontSize="6" pageId="6" pageNumber="7">R</subScript>
= right side muscles; IM
<subScript id="178F349EFFADFF971E83E5EAFB1BF816" attach="left" box="[1194,1203,1982,1996]" fontSize="6" pageId="6" pageNumber="7">1</subScript>
and IM
<subScript id="178F349EFFADFF971F39E5EAFAB1F816" attach="left" box="[1296,1305,1982,1996]" fontSize="6" pageId="6" pageNumber="7">2</subScript>
= intermediate muscles.
</paragraph>
</caption>
<paragraph id="8BB436DBFFACFF961AC3E35FFE67FEFF" blockId="7.[189,612,267,328]" box="[234,463,267,293]" pageId="7" pageNumber="8">
<emphasis id="B97FEAC9FFACFF961AC3E35FFE67FEFF" bold="true" box="[234,463,267,293]" pageId="7" pageNumber="8">Dextral individual</emphasis>
</paragraph>
<paragraph id="8BB436DBFFACFF961A94E37AFDCCFE92" blockId="7.[189,612,267,328]" box="[189,612,302,328]" pageId="7" pageNumber="8">Mirror image of sinistral individual.</paragraph>
</subSubSection>
<subSubSection id="C3116550FFACFF961A94E324FD05FE06" pageId="7" pageNumber="8" type="etymology">
<paragraph id="8BB436DBFFACFF961A94E324FEECFE50" blockId="7.[189,324,368,394]" box="[189,324,368,394]" pageId="7" pageNumber="8">
<heading id="D0FC81B7FFACFF961A94E324FEECFE50" bold="true" box="[189,324,368,394]" fontSize="11" level="3" pageId="7" pageNumber="8" reason="3">
<emphasis id="B97FEAC9FFACFF961A94E324FEECFE50" bold="true" box="[189,324,368,394]" pageId="7" pageNumber="8">Etymology</emphasis>
</heading>
</paragraph>
<paragraph id="8BB436DBFFACFF961A94E3CAFD05FE06" blockId="7.[189,1398,414,476]" pageId="7" pageNumber="8">
<taxonomicName id="4C0B4D58FFACFF961A94E3CAFE32FE62" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[189,410,414,440]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="7" pageNumber="8" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFACFF961A94E3CAFE32FE62" box="[189,410,414,440]" italics="true" pageId="7" pageNumber="8">Brooksia lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFACFF961B88E3CBFE53FE63" box="[417,507,415,441]" pageId="7" pageNumber="8" rank="species">sp. nov.</taxonomicNameLabel>
is named after the island of Lokrum near which it has been found. The Latin name of the island of Lokrum is Lacroma.
</paragraph>
</subSubSection>
<subSubSection id="C3116550FFACFF991A94E056FCA1FBB3" lastPageId="8" lastPageNumber="9" pageId="7" pageNumber="8" type="materials_examined">
<paragraph id="8BB436DBFFACFF961A94E056FE02FDC6" blockId="7.[189,426,514,540]" box="[189,426,514,540]" pageId="7" pageNumber="8">
<heading id="D0FC81B7FFACFF961A94E056FE02FDC6" bold="true" box="[189,426,514,540]" fontSize="11" level="3" pageId="7" pageNumber="8" reason="3">
<emphasis id="B97FEAC9FFACFF961A94E056FE02FDC6" bold="true" box="[189,426,514,540]" pageId="7" pageNumber="8">Material examined</emphasis>
</heading>
</paragraph>
<paragraph id="8BB436DBFFACFF961A94E066FDF2FD91" blockId="7.[189,602,561,588]" box="[189,602,561,588]" pageId="7" pageNumber="8">
<materialsCitation id="3B633C86FFACFF961A94E066FDF2FD91" ID-GBIF-Occurrence="2621710302" box="[189,602,561,588]" pageId="7" pageNumber="8">Two oozooids and 19 blastozooids.</materialsCitation>
</paragraph>
<materialsCitation id="3B633C86FFACFF961AC3E027FB30FD2C" ID-GBIF-Occurrence="2621710301" pageId="7" pageNumber="8" typeStatus="holotype">
<paragraph id="8BB436DBFFACFF961AC3E027FEF4FD57" blockId="7.[189,1398,627,759]" box="[234,348,627,653]" pageId="7" pageNumber="8">
<emphasis id="B97FEAC9FFACFF961AC3E027FEF4FD57" bold="true" box="[234,348,627,653]" pageId="7" pageNumber="8">
<typeStatus id="54B08879FFACFF961AC3E027FEF4FD57" box="[234,348,627,653]" pageId="7" pageNumber="8" type="holotype">Holotype</typeStatus>
</emphasis>
</paragraph>
<paragraph id="8BB436DBFFACFF961A94E0C2FB30FD2C" blockId="7.[189,1398,627,759]" pageId="7" pageNumber="8">
One oozooid (
<quantity id="4CF39B3EFFACFF961B4CE0C2FE78FD6A" box="[357,464,662,688]" metricMagnitude="-2" metricUnit="m" metricValue="1.48" pageId="7" pageNumber="8" unit="mm" value="14.8">14.8 mm</quantity>
body length,
<quantity id="4CF39B3EFFACFF96185AE0C2FD75FD6A" box="[627,733,662,688]" metricMagnitude="-2" metricUnit="m" metricValue="2.3899999999999997" pageId="7" pageNumber="8" unit="mm" value="23.9">23.9 mm</quantity>
total length including proboscis), collected from a
<quantity id="4CF39B3EFFACFF961F15E0C2FF7CFD0E" metricMagnitude="1" metricUnit="m" metricValue="2.5" metricValueMax="5.0" metricValueMin="0.0" pageId="7" pageNumber="8" unit="m" value="25.0" valueMax="50.0" valueMin="0.0">050 m</quantity>
depth layer on
<date id="FFB5101BFFACFF961BA0E0EDFDBBFD09" box="[393,531,697,723]" pageId="7" pageNumber="8" value="2014-10-03">
3 Oct.
<quantity id="4CF39B3EFFACFF961BF1E0EDFDBBFD09" box="[472,531,697,723]" metricMagnitude="1" metricUnit="m" metricValue="5.11556" pageId="7" pageNumber="8" unit="in" value="2014.0">2014</quantity>
</date>
in a 53 μm mesh plankton net. It is deposited in the Tunicata collection of the Croatian Natural History Museum under inventory number
<collectionCode id="ED1AAE1EFFACFF96198DE088FC54FD2C" box="[932,1020,732,758]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:33139" lsid="urn:lsid:biocol.org:col:33139" name="Cincinnati Museum of Natural History" pageId="7" pageNumber="8" type="Museum">CNHM</collectionCode>
Inv. br. 44/1.
</paragraph>
</materialsCitation>
<caption id="DF746653FFACFF961A94E532FCCAF831" ID-DOI="http://doi.org/10.5281/zenodo.3837960" ID-Zenodo-Dep="3837960" httpUri="https://zenodo.org/record/3837960/files/figure.png" pageId="7" pageNumber="8" startId="7.[189,231,1894,1920]" targetBox="[275,1315,802,1848]" targetPageId="7">
<paragraph id="8BB436DBFFACFF961A94E532FCCAF831" blockId="7.[189,1399,1894,2027]" pageId="7" pageNumber="8">
<emphasis id="B97FEAC9FFACFF961A94E532FEB9F85A" bold="true" box="[189,273,1894,1920]" pageId="7" pageNumber="8">Fig. 4.</emphasis>
<taxonomicName id="4C0B4D58FFACFF961B36E532FDAAF85A" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[287,514,1894,1920]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="7" pageNumber="8" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFACFF961B36E532FDAAF85A" box="[287,514,1894,1920]" italics="true" pageId="7" pageNumber="8">Brooksia lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFACFF961839E533FDD9F85B" box="[528,625,1895,1921]" pageId="7" pageNumber="8" rank="species">sp. nov.</taxonomicNameLabel>
<emphasis id="B97FEAC9FFACFF961856E532FD3CF85A" bold="true" box="[639,660,1894,1920]" pageId="7" pageNumber="8">A</emphasis>
. Photograph of the ventral side of the dextral blastozooid.
<emphasis id="B97FEAC9FFACFF961A94E5DEFF78F87E" bold="true" box="[189,208,1930,1956]" pageId="7" pageNumber="8">B</emphasis>
. Photograph of the dorsal side of the sinistral blastozooid.
<emphasis id="B97FEAC9FFACFF961980E5DDFC16F879" bold="true" box="[937,958,1929,1955]" pageId="7" pageNumber="8">C</emphasis>
. Photograph of the lateral side of a blastozooid.
<emphasis id="B97FEAC9FFACFF961B7BE5F9FECFF81D" bold="true" box="[338,359,1965,1991]" pageId="7" pageNumber="8">D</emphasis>
. Detail of the ventral posterior end of an oozooid. All indivuduals were dyed with Janus Green dye. Abbreviations: st = stolon; tp = test processus.
</paragraph>
</caption>
<paragraph id="8BB436DBFFA3FF991AC3E35FFEFCFEFF" blockId="8.[189,1399,267,399]" box="[234,340,267,293]" pageId="8" pageNumber="9">
<emphasis id="B97FEAC9FFA3FF991AC3E35FFEFCFEFF" bold="true" box="[234,340,267,293]" pageId="8" pageNumber="9">
<typeStatus id="54B08879FFA3FF991AC3E35FFEFCFEFF" box="[234,340,267,293]" pageId="8" pageNumber="9" type="allotype">Allotype</typeStatus>
</emphasis>
</paragraph>
<paragraph id="8BB436DBFFA3FF991A94E37AFBCEFE55" blockId="8.[189,1399,267,399]" pageId="8" pageNumber="9">
One sinistral blastozooid (
<quantity id="4CF39B3EFFA3FF991BC6E37AFDE2FE93" box="[495,586,302,329]" metricMagnitude="-3" metricUnit="m" metricValue="4.2" pageId="8" pageNumber="9" unit="mm" value="4.2">4.2 mm</quantity>
body length, without attachment processes), collected from a
<quantity id="4CF39B3EFFA3FF991F37E37AFADFFE93" box="[1310,1399,302,329]" metricMagnitude="1" metricUnit="m" metricValue="2.5" metricValueMax="5.0" metricValueMin="0.0" pageId="8" pageNumber="9" unit="m" value="25.0" valueMax="50.0" valueMin="0.0">050 m</quantity>
depth layer on
<date id="FFB5101BFFA3FF991B43E305FE5AFEB1" box="[362,498,337,363]" pageId="8" pageNumber="9" value="2014-10-03">
3 Oct.
<quantity id="4CF39B3EFFA3FF991B9EE305FE5AFEB1" box="[439,498,337,363]" metricMagnitude="1" metricUnit="m" metricValue="5.11556" pageId="8" pageNumber="9" unit="in" value="2014.0">2014</quantity>
</date>
in a 53 μm mesh plankton net. It is deposited in the Tunicata collection of the Croatian Natural History Museum under inventory number
<collectionCode id="ED1AAE1EFFA3FF991950E321FC79FE55" box="[889,977,373,399]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:33139" lsid="urn:lsid:biocol.org:col:33139" name="Cincinnati Museum of Natural History" pageId="8" pageNumber="9" type="Museum">CNHM</collectionCode>
Inv. br. 44/2
</paragraph>
<paragraph id="8BB436DBFFA3FF991AC3E3E2FECFFE0A" blockId="8.[189,1399,438,711]" box="[234,359,438,464]" pageId="8" pageNumber="9">
<emphasis id="B97FEAC9FFA3FF991AC3E3E2FECFFE0A" bold="true" box="[234,359,438,464]" pageId="8" pageNumber="9">
<typeStatus id="54B08879FFA3FF991AC3E3E2FECFFE0A" box="[234,359,438,464]" pageId="8" pageNumber="9" type="paratype">Paratypes</typeStatus>
</emphasis>
</paragraph>
<paragraph id="8BB436DBFFA3FF991A94E38DFE0FFD1C" blockId="8.[189,1399,438,711]" pageId="8" pageNumber="9">
One oozooid (
<quantity id="4CF39B3EFFA3FF991B58E38DFE49FE2E" box="[369,481,473,500]" metricMagnitude="-2" metricUnit="m" metricValue="1.15" pageId="8" pageNumber="9" unit="mm" value="11.5">11.5 mm</quantity>
body length,
<quantity id="4CF39B3EFFA3FF9918BEE38DFCAEFE2E" box="[663,774,473,500]" metricMagnitude="-2" metricUnit="m" metricValue="1.8199999999999998" pageId="8" pageNumber="9" unit="mm" value="18.2">18.2 mm</quantity>
total length including proboscis) and one dextral blastozooid (
<quantity id="4CF39B3EFFA3FF991B7EE3A8FE1FFDCD" box="[343,439,508,535]" metricMagnitude="-3" metricUnit="m" metricValue="4.8" pageId="8" pageNumber="9" unit="mm" value="4.8">4.8 mm</quantity>
body length, without attachment processes), collected from a
<quantity id="4CF39B3EFFA3FF991E99E3A8FA82FDCD" box="[1200,1322,508,535]" metricMagnitude="1" metricUnit="m" metricValue="7.5" metricValueMax="10.0" metricValueMin="5.0" pageId="8" pageNumber="9" unit="m" value="75.0" valueMax="100.0" valueMin="50.0">50100 m</quantity>
depth layer on
<date id="FFB5101BFFA3FF991B0AE074FE04FDE0" box="[291,428,544,570]" pageId="8" pageNumber="9" value="2014-10-03">3 Oct. 2014</date>
using a 53 μm mesh plankton net, deposited in the Institute for Coastal and Marine Research (University of Dubrovnik) under inventory number IMP-002; one dextral blastozooid (
<quantity id="4CF39B3EFFA3FF991F78E017FF43FD5A" metricMagnitude="-3" metricUnit="m" metricValue="4.1" pageId="8" pageNumber="9" unit="mm" value="4.1">4.1 mm</quantity>
body length, without attachment processes) collected from a
<quantity id="4CF39B3EFFA3FF9919E6E032FB82FD5A" box="[975,1066,614,641]" metricMagnitude="1" metricUnit="m" metricValue="2.5" metricValueMax="5.0" metricValueMin="0.0" pageId="8" pageNumber="9" unit="m" value="25.0" valueMax="50.0" valueMin="0.0">050 m</quantity>
depth layer on
<date id="FFB5101BFFA3FF991EC0E032FADFFD5A" box="[1257,1399,614,640]" pageId="8" pageNumber="9" value="2014-10-03">3 Oct. 2014</date>
with a 200 μm mesh plankton net, deposited in the Dubrovnik Natural History Museum under inventory number PMD 2106.
</paragraph>
<paragraph id="8BB436DBFFA3FF991AC3E0BAFE0EFCD2" blockId="8.[189,1399,750,846]" box="[234,422,750,776]" pageId="8" pageNumber="9">
<emphasis id="B97FEAC9FFA3FF991AC3E0BAFE0EFCD2" bold="true" box="[234,422,750,776]" pageId="8" pageNumber="9">Other material</emphasis>
</paragraph>
<paragraph id="8BB436DBFFA3FF991A94E145FDFAFC94" blockId="8.[189,1399,750,846]" pageId="8" pageNumber="9">16 blastozooids deposited in the Institute for Coastal and Marine research (University of Dubrovnik) under inventory number IMP-003.</paragraph>
<paragraph id="8BB436DBFFA3FF991A94E122FEF5FC55" blockId="8.[189,349,885,912]" box="[189,349,885,912]" pageId="8" pageNumber="9">
<heading id="D0FC81B7FFA3FF991A94E122FEF5FC55" bold="true" box="[189,349,885,912]" fontSize="11" level="3" pageId="8" pageNumber="9" reason="3">
<emphasis id="B97FEAC9FFA3FF991A94E122FEF5FC55" bold="true" box="[189,349,885,912]" pageId="8" pageNumber="9">Type locality</emphasis>
</heading>
</paragraph>
<paragraph id="8BB436DBFFA3FF991A94E1F0FB06FC64" blockId="8.[189,1198,932,959]" box="[189,1198,932,959]" pageId="8" pageNumber="9">
<geoCoordinate id="EE3F501CFFA3FF991A94E1F0FEE2FC64" box="[189,330,932,958]" degrees="42" direction="north" minutes="37" orientation="latitude" pageId="8" pageNumber="9" precision="15" seconds="21" value="42.622498">42°37'21&quot;N</geoCoordinate>
,
<geoCoordinate id="EE3F501CFFA3FF991B7FE1F0FE46FC65" box="[342,494,932,959]" degrees="018" direction="east" minutes="06" orientation="longitude" pageId="8" pageNumber="9" precision="15" seconds="05" value="18.101389">018°06'05&quot;E</geoCoordinate>
, off Dubrovnik, South Adriatic (Mediterranean Sea;
<figureCitation id="13302A5EFFA3FF991E71E1F0FB34FC64" box="[1112,1180,932,958]" captionStart="Fig" captionStartId="1.[189,232,1964,1990]" captionTargetBox="[193,1400,1389,1912]" captionTargetId="figure@1.[187,1409,1376,1923]" captionTargetPageId="1" captionText="Fig. 1. Position of the Lokrum station in the South Adriatic, where specimens of Brooksia lacromae sp. nov. were collected." figureDoi="http://doi.org/10.5281/zenodo.3837954" httpUri="https://zenodo.org/record/3837954/files/figure.png" pageId="8" pageNumber="9">Fig. 1</figureCitation>
).
</paragraph>
<paragraph id="8BB436DBFFA3FF991A94E1B1FCA1FBB3" blockId="8.[189,1399,997,1130]" pageId="8" pageNumber="9">
The temperature average in the
<quantity id="4CF39B3EFFA3FF991814E1B1FD30FBDA" box="[573,664,997,1024]" metricMagnitude="1" metricUnit="m" metricValue="2.5" metricValueMax="5.0" metricValueMin="0.0" pageId="8" pageNumber="9" unit="m" value="25.0" valueMax="50.0" valueMin="0.0">050 m</quantity>
layer was 19.0°C, with a range between 16.2°C and 22.2°C, while the salinity average was 38.36, with a range between 37.79 and 38.68. In the
<quantity id="4CF39B3EFFA3FF991EB6E65DFAAFFBF9" box="[1183,1287,1033,1060]" metricMagnitude="1" metricUnit="m" metricValue="7.0" metricValueMax="9.0" metricValueMin="5.0" pageId="8" pageNumber="9" unit="m" value="70.0" valueMax="90.0" valueMin="50.0">5090 m</quantity>
layer the temperature average was 15.8°C, with a range between 15.5°C and 16.1°C, while the salinity average was 38.71, with a range between 38.67 and 38.77.
</paragraph>
</subSubSection>
<subSubSection id="C3116550FFA3FF981A94E6C5FCCAFCE6" lastPageId="9" lastPageNumber="10" pageId="8" pageNumber="9" type="discussion">
<paragraph id="8BB436DBFFA3FF991A94E6C5FE87FB71" blockId="8.[189,303,1169,1195]" box="[189,303,1169,1195]" pageId="8" pageNumber="9">
<heading id="D0FC81B7FFA3FF991A94E6C5FE87FB71" bold="true" box="[189,303,1169,1195]" fontSize="11" level="3" pageId="8" pageNumber="9" reason="3">
<emphasis id="B97FEAC9FFA3FF991A94E6C5FE87FB71" bold="true" box="[189,303,1169,1195]" pageId="8" pageNumber="9">Remarks</emphasis>
</heading>
</paragraph>
<paragraph id="8BB436DBFFA3FF991A94E6EBFADFF8D6" blockId="8.[189,1399,1215,1805]" pageId="8" pageNumber="9">
Oozooid.
<taxonomicName id="4C0B4D58FFA3FF991B1AE6EBFDAAFB03" baseAuthorityName="van Soest" baseAuthorityYear="1975" box="[307,514,1215,1241]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="rostrata">
<emphasis id="B97FEAC9FFA3FF991B1AE6EBFDAAFB03" box="[307,514,1215,1241]" italics="true" pageId="8" pageNumber="9">Brooksia rostrata</emphasis>
</taxonomicName>
and
<taxonomicName id="4C0B4D58FFA3FF991816E6EBFCA0FB03" authorityName="van Soest" authorityYear="1975" box="[575,776,1215,1241]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="berneri">
<emphasis id="B97FEAC9FFA3FF991816E6EBFCA0FB03" box="[575,776,1215,1241]" italics="true" pageId="8" pageNumber="9">Brooksia berneri</emphasis>
</taxonomicName>
oozooids are very similar, differing only in the fact that in
<taxonomicName id="4C0B4D58FFA3FF991B27E6B7FE2DFB26" authorityName="van Soest" authorityYear="1975" box="[270,389,1250,1276]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="berneri">
<emphasis id="B97FEAC9FFA3FF991B27E6B7FE2DFB26" box="[270,389,1250,1276]" italics="true" pageId="8" pageNumber="9">B. berneri</emphasis>
</taxonomicName>
oozooid muscle I, joined with the intermediate muscle (im), is separated from muscle II and discontinuous mid-dorsally. Due to this fact we will only compare the
<taxonomicName id="4C0B4D58FFA3FF991E53E752FAA5FAFA" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[1146,1293,1286,1312]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFA3FF991E53E752FAA5FAFA" box="[1146,1293,1286,1312]" italics="true" pageId="8" pageNumber="9">B. lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFA3FF991F31E752FADFFAFA" box="[1304,1399,1286,1312]" pageId="8" pageNumber="9" rank="species">sp. nov.</taxonomicNameLabel>
oozooid to the
<taxonomicName id="4C0B4D58FFA3FF991B40E77EFE4EFA99" baseAuthorityName="van Soest" baseAuthorityYear="1975" box="[361,486,1322,1347]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="rostrata">
<emphasis id="B97FEAC9FFA3FF991B40E77EFE4EFA99" box="[361,486,1322,1347]" italics="true" pageId="8" pageNumber="9">B. rostrata</emphasis>
</taxonomicName>
oozooid. The
<taxonomicName id="4C0B4D58FFA3FF9918A5E77DFCC1FA99" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[652,873,1321,1347]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFA3FF9918A5E77DFCC1FA99" box="[652,873,1321,1347]" italics="true" pageId="8" pageNumber="9">Brooksia lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFA3FF991946E77DFC60FA99" box="[879,968,1321,1347]" pageId="8" pageNumber="9" rank="species">sp. nov.</taxonomicNameLabel>
oozooid has one ventral longitudinal muscle which extends into the proboscis, while
<taxonomicName id="4C0B4D58FFA3FF9918D4E719FCD4FABC" baseAuthorityName="van Soest" baseAuthorityYear="1975" box="[765,892,1357,1382]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="rostrata">
<emphasis id="B97FEAC9FFA3FF9918D4E719FCD4FABC" box="[765,892,1357,1382]" italics="true" pageId="8" pageNumber="9">B. rostrata</emphasis>
</taxonomicName>
has two (
<figureCitation id="13302A5EFFA3FF9919DCE718FB96FABC" box="[1013,1086,1356,1382]" captionStart="Fig" captionStartId="5.[189,232,1789,1815]" captionTargetBox="[235,1328,407,1719]" captionTargetId="figure@5.[219,1353,390,1747]" captionTargetPageId="5" captionText="Fig. 2. Oozooid of Brooksia lacromae sp. nov. A. Dorsal view, drawing. B. Ventral view, drawing. C. Dorsal view, photograph of an individual dyed with Janus Green, with an arrow indicating breakage in the proboscis during collection. Abbreviations: as = atrial siphon; bl = branching of the ventral longitudinal muscle; br = branchial bar; dt = dorsal tubercle; en = endostyle; g = ganglion; im = intermediate muscle; lm = longitudinal muscle; lo = openings in the longitudinal muscle; nu = nucleus; os = oral siphon; p = proboscis; pb = peripharyngeal band; st = stolon; tp = test processus; IVII = body muscles." figureDoi="http://doi.org/10.5281/zenodo.3837956" httpUri="https://zenodo.org/record/3837956/files/figure.png" pageId="8" pageNumber="9">Fig. 2</figureCitation>
). The proboscides in both collected oozooids broke off during collection, but were found in the sample (
<figureCitation id="13302A5EFFA3FF991E60E73BFB36FA53" box="[1097,1182,1391,1417]" captionStart="Fig" captionStartId="5.[189,232,1789,1815]" captionTargetBox="[235,1328,407,1719]" captionTargetId="figure@5.[219,1353,390,1747]" captionTargetPageId="5" captionText="Fig. 2. Oozooid of Brooksia lacromae sp. nov. A. Dorsal view, drawing. B. Ventral view, drawing. C. Dorsal view, photograph of an individual dyed with Janus Green, with an arrow indicating breakage in the proboscis during collection. Abbreviations: as = atrial siphon; bl = branching of the ventral longitudinal muscle; br = branchial bar; dt = dorsal tubercle; en = endostyle; g = ganglion; im = intermediate muscle; lm = longitudinal muscle; lo = openings in the longitudinal muscle; nu = nucleus; os = oral siphon; p = proboscis; pb = peripharyngeal band; st = stolon; tp = test processus; IVII = body muscles." figureDoi="http://doi.org/10.5281/zenodo.3837956" httpUri="https://zenodo.org/record/3837956/files/figure.png" pageId="8" pageNumber="9">Fig. 2c</figureCitation>
). The proboscis of
<taxonomicName id="4C0B4D58FFA3FF991A94E7C6FE33FA76" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[189,411,1426,1452]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFA3FF991A94E7C6FE33FA76" box="[189,411,1426,1452]" italics="true" pageId="8" pageNumber="9">Brooksia lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFA3FF991B88E7C7FE53FA77" box="[417,507,1427,1453]" pageId="8" pageNumber="9" rank="species">sp. nov.</taxonomicNameLabel>
seems to be thinner at the base than in
<taxonomicName id="4C0B4D58FFA3FF9919EDE7C6FB38FA76" baseAuthorityName="van Soest" baseAuthorityYear="1975" box="[964,1168,1426,1452]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="rostrata">
<emphasis id="B97FEAC9FFA3FF9919EDE7C6FB38FA76" box="[964,1168,1426,1452]" italics="true" pageId="8" pageNumber="9">Brooksia rostrata</emphasis>
</taxonomicName>
and in both species it contains two lateral longitudinal muscles. The ventral longitudinal muscle in
<taxonomicName id="4C0B4D58FFA3FF991E57E7E2FAB8FA0A" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[1150,1296,1462,1488]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFA3FF991E57E7E2FAB8FA0A" box="[1150,1296,1462,1488]" italics="true" pageId="8" pageNumber="9">B. lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFA3FF991F30E7E2FADFFA0A" box="[1305,1399,1462,1488]" pageId="8" pageNumber="9" rank="species">sp. nov.</taxonomicNameLabel>
has two small slit-like openings anteriorly, one below the endostyle and the other just anteriorly to it, partly overlapping with the anterior end of the endostyle. In
<taxonomicName id="4C0B4D58FFA3FF9919A3E7A8FBC1F9CC" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[906,1129,1532,1558]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFA3FF9919A3E7A8FBC1F9CC" box="[906,1129,1532,1558]" italics="true" pageId="8" pageNumber="9">Brooksia lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFA3FF991E58E7A8FB67F9CC" box="[1137,1231,1532,1558]" pageId="8" pageNumber="9" rank="species">sp. nov.</taxonomicNameLabel>
, in contrast to
<taxonomicName id="4C0B4D58FFA3FF991A94E474FE95F9E3" baseAuthorityName="van Soest" baseAuthorityYear="1975" box="[189,317,1568,1593]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="rostrata">
<emphasis id="B97FEAC9FFA3FF991A94E474FE95F9E3" box="[189,317,1568,1593]" italics="true" pageId="8" pageNumber="9">B. rostrata</emphasis>
</taxonomicName>
, body muscles are not arranged in a barrel-like structure where muscles are perpendicular to the body axis dorsally, as well as ventrally, but they converge to the posterior third of the body. Only muscles I, VI and VII fuse with the branches of the ventral longitudinal muscle, while muscles II, III, IV and V converge to the connection between muscle I and branches of the ventral longitudinal muscle, without fusing with either. Only muscle II can sometimes be slightly connected with muscle I ventrally. The stolon in
<taxonomicName id="4C0B4D58FFA3FF991B4DE49BFDECF933" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[356,580,1743,1769]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFA3FF991B4DE49BFDECF933" box="[356,580,1743,1769]" italics="true" pageId="8" pageNumber="9">Brooksia lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFA3FF991865E484FD00F930" box="[588,680,1744,1770]" pageId="8" pageNumber="9" rank="species">sp. nov.</taxonomicNameLabel>
seems to emerge from the test, slightly posteriorly from the nucleus (
<figureCitation id="13302A5EFFA3FF991B0CE4A6FE2AF8D6" box="[293,386,1778,1804]" captionStart="Fig" captionStartId="7.[189,231,1894,1920]" captionTargetBox="[275,1315,802,1848]" captionTargetId="figure@7.[257,1315,790,1858]" captionTargetPageId="7" captionText="Fig. 4. Brooksia lacromae sp. nov. A. Photograph of the ventral side of the dextral blastozooid. B. Photograph of the dorsal side of the sinistral blastozooid. C. Photograph of the lateral side of a blastozooid. D. Detail of the ventral posterior end of an oozooid. All indivuduals were dyed with Janus Green dye. Abbreviations: st = stolon; tp = test processus." figureDoi="http://doi.org/10.5281/zenodo.3837960" httpUri="https://zenodo.org/record/3837960/files/figure.png" pageId="8" pageNumber="9">Fig. 4D</figureCitation>
), while in
<taxonomicName id="4C0B4D58FFA3FF991BD0E4A7FDDDF8D6" baseAuthorityName="van Soest" baseAuthorityYear="1975" box="[505,629,1779,1804]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="rostrata">
<emphasis id="B97FEAC9FFA3FF991BD0E4A7FDDDF8D6" box="[505,629,1779,1804]" italics="true" pageId="8" pageNumber="9">B. rostrata</emphasis>
</taxonomicName>
it emerges from the anterior part of the nucleus (
<bibRefCitation id="EF9A4B2AFFA3FF991E8CE4A6FAC2F8D6" author="Thompson H." box="[1189,1386,1778,1804]" pageId="8" pageNumber="9" refId="ref6993" refString="Thompson H. 1948. Pelagic Tunicates of Australia. Commonwealth Council for Scientific and Industrial Research, Melbourne." type="book" year="1948">Thompson 1948</bibRefCitation>
).
</paragraph>
<paragraph id="8BB436DBFFA3FF981A94E560FE53FE22" blockId="8.[189,1399,1844,2011]" lastBlockId="9.[189,1399,267,505]" lastPageId="9" lastPageNumber="10" pageId="8" pageNumber="9">
Blastozooid (sinistral individual). The
<taxonomicName id="4C0B4D58FFA3FF9918A3E560FCC2F894" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[650,874,1844,1870]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFA3FF9918A3E560FCC2F894" box="[650,874,1844,1870]" italics="true" pageId="8" pageNumber="9">Brooksia lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFA3FF99195BE560FC67F894" box="[882,975,1844,1870]" pageId="8" pageNumber="9" rank="species">sp. nov.</taxonomicNameLabel>
and
<taxonomicName id="4C0B4D58FFA3FF991E22E560FB72F894" baseAuthorityName="van Soest" baseAuthorityYear="1975" box="[1035,1242,1844,1870]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="rostrata">
<emphasis id="B97FEAC9FFA3FF991E22E560FB72F894" box="[1035,1242,1844,1870]" italics="true" pageId="8" pageNumber="9">Brooksia rostrata</emphasis>
</taxonomicName>
blastozooids are very similar. In
<taxonomicName id="4C0B4D58FFA3FF991B85E503FD25F8AB" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[428,653,1879,1905]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFA3FF991B85E503FD25F8AB" box="[428,653,1879,1905]" italics="true" pageId="8" pageNumber="9">Brooksia lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFA3FF9918BFE50CFD5EF8A8" box="[662,758,1880,1906]" pageId="8" pageNumber="9" rank="species">sp. nov.</taxonomicNameLabel>
, muscle IIR-b ends blindly on the ventral side of the animal without entering the posterior attachment processus (ap3), while in
<taxonomicName id="4C0B4D58FFA3FF991E37E52EFB42F84E" baseAuthorityName="van Soest" baseAuthorityYear="1975" box="[1054,1258,1914,1940]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="rostrata">
<emphasis id="B97FEAC9FFA3FF991E37E52EFB42F84E" box="[1054,1258,1914,1940]" italics="true" pageId="8" pageNumber="9">Brooksia rostrata</emphasis>
</taxonomicName>
it enters the posterior attachment processus (ap3). The left intermediate muscle (IM1) in
<taxonomicName id="4C0B4D58FFA3FF991E11E5C9FABEF86D" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[1080,1302,1949,1975]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="8" pageNumber="9" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFA3FF991E11E5C9FABEF86D" box="[1080,1302,1949,1975]" italics="true" pageId="8" pageNumber="9">Brooksia lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFA3FF991F34E5CAFADFF862" box="[1309,1399,1950,1976]" pageId="8" pageNumber="9" rank="species">sp. nov.</taxonomicNameLabel>
is continuous dorsally and ventrally. Its branch IM1-a enters the anterior attachment processus (ap1). In
<taxonomicName id="4C0B4D58FFA2FF981A94E35FFE22FEFF" baseAuthorityName="van Soest" baseAuthorityYear="1975" box="[189,394,267,293]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="9" pageNumber="10" phylum="Chordata" rank="species" species="rostrata">
<emphasis id="B97FEAC9FFA2FF981A94E35FFE22FEFF" box="[189,394,267,293]" italics="true" pageId="9" pageNumber="10">Brooksia rostrata</emphasis>
</taxonomicName>
the left intermediate muscle is discontinuous. Its dorsal and ventral counterparts both enter the anterior attachment processus. Because of this, the anterior attachment processus in
<taxonomicName id="4C0B4D58FFA2FF981EC3E37BFADFFE93" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[1258,1399,303,329]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="9" pageNumber="10" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFA2FF981EC3E37BFADFFE93" box="[1258,1399,303,329]" italics="true" pageId="9" pageNumber="10">B. lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFA2FF981A94E306FEBFFEB6" box="[189,279,338,364]" pageId="9" pageNumber="10" rank="species">sp. nov.</taxonomicNameLabel>
possesses two muscles (IM1-a and IM2), while in
<taxonomicName id="4C0B4D58FFA2FF981943E307FC4FFEB6" baseAuthorityName="van Soest" baseAuthorityYear="1975" box="[874,999,339,364]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="9" pageNumber="10" phylum="Chordata" rank="species" species="rostrata">
<emphasis id="B97FEAC9FFA2FF981943E307FC4FFEB6" box="[874,999,339,364]" italics="true" pageId="9" pageNumber="10">B. rostrata</emphasis>
</taxonomicName>
it possesses three muscles (dorsal and ventral left intermediate muscle and right intermediate muscle). According to
<bibRefCitation id="EF9A4B2AFFA2FF981EB3E321FADAFE55" author="Thompson H." box="[1178,1394,373,399]" pageId="9" pageNumber="10" refId="ref6993" refString="Thompson H. 1948. Pelagic Tunicates of Australia. Commonwealth Council for Scientific and Industrial Research, Melbourne." type="book" year="1948">Thompson (1948)</bibRefCitation>
, the right intermediate muscle of the sinistral individual of
<taxonomicName id="4C0B4D58FFA2FF981944E3CDFC43FE68" baseAuthorityName="van Soest" baseAuthorityYear="1975" box="[877,1003,409,434]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="9" pageNumber="10" phylum="Chordata" rank="species" species="rostrata">
<emphasis id="B97FEAC9FFA2FF981944E3CDFC43FE68" box="[877,1003,409,434]" italics="true" pageId="9" pageNumber="10">B. rostrata</emphasis>
</taxonomicName>
ends blindly, while after
<bibRefCitation id="EF9A4B2AFFA2FF981F3EE3CCFEA3FE0C" author="Tokioka T." pageId="9" pageNumber="10" pagination="147 - 153" refId="ref7013" refString="Tokioka T. 1954. Descriptions on the aggregated form of Brooksia rostrata (Traustedt), an insufficiently known salpa. Publications of the Seto Marine Biological Laboratory 4 (1): 147 - 153." type="journal article" year="1954">Tokioka (1954)</bibRefCitation>
and
<bibRefCitation id="EF9A4B2AFFA2FF981B65E3EFFDB8FE0F" author="Godeaux J." box="[332,528,443,469]" pageId="9" pageNumber="10" pagination="273 - 294" refId="ref6293" refString="Godeaux J. 1998. The relationships and systematics of the Thaliacea, with keys for identification. In: Bone Q. (ed.) The Biology of Pelagic Tunicates: 273 - 294. Oxford University Press, Oxford." type="book chapter" year="1998">Godeaux (1998)</bibRefCitation>
it connects to muscle I. In
<taxonomicName id="4C0B4D58FFA2FF981943E3E8FC55FE0F" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[874,1021,443,469]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="9" pageNumber="10" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFA2FF981943E3E8FC55FE0F" box="[874,1021,443,469]" italics="true" pageId="9" pageNumber="10">B. lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFA2FF981E21E3E8FBCFFE0C" box="[1032,1127,444,470]" pageId="9" pageNumber="10" rank="species">sp. nov.</taxonomicNameLabel>
the right intermediate muscle (IM2) ends blindly.
</paragraph>
<paragraph id="8BB436DBFFA2FF981A94E074FE2FFDE0" blockId="9.[189,391,544,570]" box="[189,391,544,570]" pageId="9" pageNumber="10">
<heading id="D0FC81B7FFA2FF981A94E074FE2FFDE0" bold="true" box="[189,391,544,570]" fontSize="11" level="3" pageId="9" pageNumber="10" reason="3">
<emphasis id="B97FEAC9FFA2FF981A94E074FE2FFDE0" bold="true" box="[189,391,544,570]" pageId="9" pageNumber="10">Genetic analysis</emphasis>
</heading>
</paragraph>
<paragraph id="8BB436DBFFA2FF981A94E01AFCCAFCE6" blockId="9.[189,1399,590,828]" pageId="9" pageNumber="10">
There were no differences between blastozooid and oozooid 18S sequences. Between two
<emphasis id="B97FEAC9FFA2FF981EEAE01BFB53FDB3" box="[1219,1275,591,617]" italics="true" pageId="9" pageNumber="10">cox1</emphasis>
sequences there were 7 substitutions out of 837 nucleotides, which results in 0.84% uncorrected pairwise distance.
<emphasis id="B97FEAC9FFA2FF981A94E0C1FF53FD75" box="[189,251,661,687]" italics="true" pageId="9" pageNumber="10">Cox1</emphasis>
sequences were translated using ascidian mitochondrial code and there were no differences in amino acid sequence between them. The uncorrected pairwise distance between the
<taxonomicName id="4C0B4D58FFA2FF981E8EE0ECFADEFD08" baseAuthorityName="van Soest" baseAuthorityYear="1975" box="[1191,1398,696,722]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="9" pageNumber="10" phylum="Chordata" rank="species" species="rostrata">
<emphasis id="B97FEAC9FFA2FF981E8EE0ECFADEFD08" box="[1191,1398,696,722]" italics="true" pageId="9" pageNumber="10">Brooksia rostrata</emphasis>
</taxonomicName>
18S sequence (
<accessionNumber id="9458AB38FFA2FF981B58E08FFE5FFD2C" box="[369,503,731,758]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/HQ015403" pageId="9" pageNumber="10">HQ015403</accessionNumber>
) and the
<taxonomicName id="4C0B4D58FFA2FF981840E08FFCE2FD2F" authority="Garić &amp; Batistić, 2016" authorityName="Garić &amp; Batistić" authorityYear="2016" box="[617,842,731,757]" class="Thaliacea" family="Salpidae" genus="Brooksia" kingdom="Animalia" order="Salpida" pageId="9" pageNumber="10" phylum="Chordata" rank="species" species="lacromae" status="sp. nov.">
<emphasis id="B97FEAC9FFA2FF981840E08FFCE2FD2F" box="[617,842,731,757]" italics="true" pageId="9" pageNumber="10">Brooksia lacromae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A24C57B2FFA2FF98197AE088FC18FD2C" box="[851,944,732,758]" pageId="9" pageNumber="10" rank="species">sp. nov.</taxonomicNameLabel>
18S sequence (
<accessionNumber id="9458AB38FFA2FF981E44E08FFB59FD2C" box="[1133,1265,731,758]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/KR057223" pageId="9" pageNumber="10">KR057223</accessionNumber>
) was 1.5% (including gaps). Out of 26 differences in 1740 nucleotides between these two sequences, there were 21 transitions, 4 transversions and one single nucleotide gap.
</paragraph>
</subSubSection>
</treatment>
</document>