treatments-xml/data/03/99/82/03998239C84B8B24FF5298C3FD4EF7CC.xml

<document id="B3C55226E6DB07A012FEB8D44797EEDD" ID-DOI="10.11646/phytotaxa.599.1.4" ID-ISSN="1179-3163" ID-Zenodo-Dep="7989211" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1685519731371" checkinUser="plazi" docAuthor="Yang, Yunhui, Liao, Chunfang, Manawasinghe, Ishara S., Farias, Antonio Roberto Gomes De &amp; Dong, Zhangyong" docDate="2023" docId="03998239C84B8B24FF5298C3FD4EF7CC" docLanguage="en" docName="phytotaxa.599.1.4.pdf" docOrigin="Phytotaxa 599 (1)" docSource="http://dx.doi.org/10.11646/phytotaxa.599.1.4" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.6:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="6" docTitle="Neomassaria khayae Y. H. Yang, C. F. Liao &amp; Manawas. 2023, sp. nov." docType="treatment" docVersion="2" lastPageNumber="70" masterDocId="FFA0FA41C84F8B21FFDA9C77FFF2FFDE" masterDocTitle="Neomassaria khayae sp. nov., a novel pleosporalean species on Khaya senegalensis from Guangdong Province, China" masterLastPageNumber="77" masterPageNumber="65" pageNumber="69" updateTime="1685520195870" updateUser="ExternalLinkService" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="CFC87CE28818ECCB516047217E69EFAC">Neomassaria khayae sp. nov., a novel pleosporalean species on Khaya senegalensis from Guangdong Province, China</mods:title>
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<mods:namePart id="8207919B43EA097508B4481F9DBA3862">Yang, Yunhui</mods:namePart>
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<mods:affiliation id="0CCB8D38BD9228C7180313901665745E">Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People’s Republic of China &amp; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; yyunhui 1226 @ 163. com; https: // orcid. org / 0000 - 0002 - 0326 - 1471</mods:affiliation>
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<mods:namePart id="5311277852361619DF522229937E6E15">Liao, Chunfang</mods:namePart>
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<mods:affiliation id="C1ABA3CB48E3848438B6A3F857B82E01">Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People’s Republic of China &amp; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; Fang 04271 @ hotmail. com; https: // orcid. org / 0000 - 0002 - 6309 - 1101</mods:affiliation>
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<mods:namePart id="6A16BF8228F78D2407BE52E0CCBA97AF">Manawasinghe, Ishara S.</mods:namePart>
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<mods:affiliation id="E2EC120499D923B00DD29FDDFA4416E5">Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People’s Republic of China &amp; ishara 9017 @ gmail. com; https: // orcid. org / 0000 - 0001 - 5730 - 3596</mods:affiliation>
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<mods:namePart id="A274DC3E4A565FEF3788469F7BA6A336">Farias, Antonio Roberto Gomes De</mods:namePart>
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<mods:affiliation id="D86BA29B92488AAD384115AC970F0F89">Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; antonio. gom @ mfu. ac. th; https: // orcid. org / 0000 - 0003 - 4768 - 1547</mods:affiliation>
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<mods:namePart id="4ABC7E5F97EF0268D30A20BCAFCD2737">Dong, Zhangyong</mods:namePart>
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<mods:affiliation id="3287A680EFEE1D2B2D364534006CF5D6">Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People’s Republic of China &amp; dongzhangyong @ hotmail. com; https: // orcid. org / 0000 - 0001 - 7524 - 0226</mods:affiliation>
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<mods:title id="C587BFE44974F689C323C745EBD8217C">Phytotaxa</mods:title>
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<mods:date id="36D7943E9586C15EA8B7DFD90A0DE0BE">2023</mods:date>
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<paragraph id="8B8F332FC84B8B25FF5298C3FC06FB10" blockId="4.[136,1012,1204,1231]" box="[136,1012,1204,1231]" pageId="4" pageNumber="69">
<heading id="D0C78443C84B8B25FF5298C3FC06FB10" box="[136,1012,1204,1231]" fontSize="11" level="2" pageId="4" pageNumber="69" reason="3">
<taxonomicName id="4C3048ACC84B8B25FF5298C3FCF3FB11" authority="Y.H. Yang, C.F. Liao &amp; Manawas." authorityName="Y. H. Yang, C. F. Liao &amp; Manawas." authorityYear="2023" box="[136,769,1204,1231]" class="Dothideomycetes" family="Massariaceae" genus="Neomassaria" kingdom="Fungi" order="Pleosporales" pageId="4" pageNumber="69" phylum="Ascomycota" rank="species" species="khayae" status="sp. nov.">
<emphasis id="B944EF3DC84B8B25FF5298C3FE8AFB11" bold="true" box="[136,376,1204,1231]" italics="true" pageId="4" pageNumber="69">Neomassaria khayae</emphasis>
Y.H. Yang, C.F. Liao &amp; Manawas.
</taxonomicName>
, 
<emphasis id="B944EF3DC84B8B25FCD198C2FC92FB10" box="[779,864,1205,1230]" italics="true" pageId="4" pageNumber="69">
<taxonomicNameLabel id="A2775246C84B8B25FCD198C2FC92FB10" box="[779,864,1205,1230]" pageId="4" pageNumber="69" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
(
<figureCitation id="130B2FAAC84B8B25FCB498C3FC19FB10" box="[878,1003,1204,1230]" captionStart="FIGURE 2" captionStartId="6.[136,229,1992,2014]" captionTargetBox="[188,1399,190,1968]" captionTargetId="figure-27@6.[188,1399,190,1968]" captionTargetPageId="6" captionText="FIGURE 2. Neomassaria khayae (ZHKU 22-0055, holotype). a–b Appearance of ascomata on the host. c Vertical section of ascoma. d Ostioles e Section through the peridium. f Pseudoparaphyses. g–h Asci. i–k Ascospores. l Germinating ascospore. m Frontal view of a colony on PDA. n Reverse view of a colony on PDA. Scale bars: c, f–h= 50 μm, d, e, i–l = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.7989217" httpUri="https://zenodo.org/record/7989217/files/figure.png" pageId="4" pageNumber="69">FIGURE 2</figureCitation>
)
</heading>
</paragraph>
<paragraph id="8B8F332FC84B8B25FF5298ABFCA8FB2C" blockId="4.[136,900,1243,1338]" box="[136,858,1243,1266]" pageId="4" pageNumber="69">
<emphasis id="B944EF3DC84B8B25FF5298ABFE7FFB2F" bold="true" box="[136,397,1244,1265]" pageId="4" pageNumber="69">Index Fungorum number</emphasis>
: IF559792, 
<emphasis id="B944EF3DC84B8B25FDD998ACFD2FFB2F" bold="true" box="[515,733,1243,1265]" pageId="4" pageNumber="69">Facesoffungi number</emphasis>
: FoF 12675.
</paragraph>
</subSubSection>
<subSubSection id="C32A60A4C84B8B25FF529977FCECF81D" pageId="4" pageNumber="69" type="etymology">
<paragraph id="8B8F332FC84B8B25FF529977FC76FAC8" blockId="4.[136,900,1243,1338]" box="[136,900,1280,1302]" pageId="4" pageNumber="69">
Etymology: In reference to the plant genus 
<emphasis id="B944EF3DC84B8B25FDF39977FD9CFACB" box="[553,622,1280,1301]" italics="true" pageId="4" pageNumber="69">
<taxonomicName id="4C3048ACC84B8B25FDF39977FD98FACB" box="[553,618,1280,1301]" class="Magnoliopsida" family="Meliaceae" genus="Khaya" kingdom="Plantae" order="Sapindales" pageId="4" pageNumber="69" phylum="Tracheophyta" rank="genus">Khaya</taxonomicName>
,
</emphasis>
which fungus was collected.
</paragraph>
<paragraph id="8B8F332FC84B8B25FF529953FE74FAE4" blockId="4.[136,900,1243,1338]" box="[136,390,1316,1338]" pageId="4" pageNumber="69">
<typeStatus id="548B8D8DC84B8B25FF529953FF17FAE4" box="[136,229,1316,1338]" pageId="4" pageNumber="69">Holotype</typeStatus>
: ZHKU 22-0055
</paragraph>
<paragraph id="8B8F332FC84B8B25FF52991FFED4F8D0" blockId="4.[136,1452,1384,2060]" pageId="4" pageNumber="69">
Saprobic on dead stem of 
<taxonomicName id="4C3048ACC84B8B25FE61991FFD6EFA5C" box="[443,668,1384,1410]" class="Magnoliopsida" family="Meliaceae" genus="Khaya" kingdom="Plantae" order="Sapindales" pageId="4" pageNumber="69" phylum="Tracheophyta" rank="species" species="senegalensis">
<emphasis id="B944EF3DC84B8B25FE61991FFD6EFA5C" box="[443,668,1384,1410]" italics="true" pageId="4" pageNumber="69">Khaya senegalensis</emphasis>
</taxonomicName>
. 
<emphasis id="B944EF3DC84B8B25FD77991FFCA5FA5C" bold="true" box="[685,855,1384,1410]" pageId="4" pageNumber="69">Sexual morph</emphasis>
: 
<emphasis id="B944EF3DC84B8B25FCB3991FFC25FA5C" box="[873,983,1384,1410]" italics="true" pageId="4" pageNumber="69">Ascomata</emphasis>
300–600× 400–600 μm (x̅ = 430 × 500 μm, n = 13), semi-immersed, solitary or gregarious, mostly globose, carbonaceous, uniloculate, dark brown to black, ostiolate, papillate. 
<emphasis id="B944EF3DC84B8B25FEB399C7FE37FA14" box="[361,453,1456,1482]" italics="true" pageId="4" pageNumber="69">Ostioles</emphasis>
central, short papillate. 
<emphasis id="B944EF3DC84B8B25FD0499C7FCB4FA14" box="[734,838,1456,1482]" italics="true" pageId="4" pageNumber="69">Peridium</emphasis>
20–100 μm wide (x̅ = 60 μm, n = 30), thick-walled, outer layer comprising 3–6 layers of brown to dark brown, thick-walled cells of 
<emphasis id="B944EF3DC84B8B25FBF999A2FB01FA30" box="[1059,1267,1493,1518]" italics="true" pageId="4" pageNumber="69">textura prismatica</emphasis>
, combined with the host tissues, inner layer comprising thin-walled, hyaline cells of 
<emphasis id="B944EF3DC84B8B25FC56998EFB91F9CC" box="[908,1123,1528,1554]" italics="true" pageId="4" pageNumber="69">textura subcircular</emphasis>
. 
<emphasis id="B944EF3DC84B8B25FBA8998FFAFDF9CC" box="[1138,1295,1528,1554]" italics="true" pageId="4" pageNumber="69">Hamathecium</emphasis>
1–2 μm wide (x̅ = 1.5 μm, n = 20), comprising branched, filamentous, septate, trabeculate pseudoparaphyses between the asci and the apex coated with a gelatinous substance. 
<emphasis id="B944EF3DC84B8B25FD1A9A37FD02F984" box="[704,752,1600,1626]" italics="true" pageId="4" pageNumber="69">Asci</emphasis>
100–150 × 15–20 μm (x̅ = 122 × 12 μm, n = 30), 8-spored, cylindrical or clavate, bitunicate, fissitunicate, apically rounded, with an ocular chamber, with a bulbous short pedicel. 
<emphasis id="B944EF3DC84B8B25FF529AFFFEF5F97C" box="[136,263,1672,1698]" italics="true" pageId="4" pageNumber="69">Ascospores</emphasis>
25–35 × 2–8 μm (x̅ = 30 × 5 μm, n = 30), overlapping-biseriate, 1–2-septate, slightly constricted at the septa, fusiform to ellipsoid, hyaline, straight or slight flexuous, tapering from the middle to both ends, surrounded no mucilaginous sheath, smooth-walled, mature ascospores contain 2–4 distinct refractive oil globules. 
<emphasis id="B944EF3DC84B8B25FB379AA7FA56F934" bold="true" box="[1261,1444,1744,1770]" pageId="4" pageNumber="69">Asexual morph</emphasis>
: Not observed.
</paragraph>
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<emphasis id="B944EF3DC84B8B25FF679B6FFE2EF8EC" bold="true" box="[189,476,1816,1842]" pageId="4" pageNumber="69">Culture characteristics:</emphasis>
—Ascospores germinated on PDA within 24 hours at room temperature (25–28˚C), colonies on PDA reaching up to 
<quantity id="4CC89ECAC84B8B25FE289B4BFDB3F889" box="[498,577,1852,1879]" metricMagnitude="-2" metricUnit="m" metricValue="2.0" pageId="4" pageNumber="69" unit="mm" value="20.0">20 mm</quantity>
diam. after three weeks at 25 °C. Above umbonate, colonies surface has black or grey irregular ring stripes, dense, slightly raised, surface not smooth, with cottony texture, the margin is irregular with crenate, brown in the centre, edges from inside to outside olivaceous black to pale brown. The reverse is dark brown to pale brown at the margin, and black in the centre.
</paragraph>
</subSubSection>
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<paragraph id="8B8F332FC84B8B25FF679BBBFA5EF7D5" blockId="4.[136,1452,1384,2060]" pageId="4" pageNumber="69">
<emphasis id="B944EF3DC84B8B25FF679BBBFE59F838" bold="true" box="[189,427,1996,2022]" pageId="4" pageNumber="69">Material examined:</emphasis>
— 
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<collectingCountry id="F32773BFC84B8B25FE1D9BBBFDFFF838" box="[455,525,1996,2022]" name="China" pageId="4" pageNumber="69">China</collectingCountry>
, 
<collectingRegion id="49F4FDCDC84B8B25FDC09BBBFCFEF838" box="[538,780,1996,2022]" country="China" name="Guangdong" pageId="4" pageNumber="69">Guangdong Province</collectingRegion>
, 
<collectingCounty id="62EE4BA3C84B8B25FCC39BBBFC27F838" box="[793,981,1996,2022]" pageId="4" pageNumber="69">Guangzhou City</collectingCounty>
, 
<collectingMunicipality id="6BEBA955C84B8B25FC389BBBFB61F838" box="[994,1171,1996,2022]" pageId="4" pageNumber="69">Haizhu District</collectingMunicipality>
, 
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(
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, 
<geoCoordinate id="EE0455E8C84B8B25FDEA9B86FD34F7D5" box="[560,710,2033,2060]" degrees="113" direction="east" minutes="16" orientation="longitude" pageId="4" pageNumber="69" precision="15" seconds="37" value="113.27695">113°16′37″ E</geoCoordinate>
, alt. 
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), on the dead stem of 
<taxonomicName id="4C3048ACC84B8B25FBF89B87FA5AF7D5" authority="(Desr.) A. Juss." authorityName="A. Juss." baseAuthorityName="Desr." box="[1058,1448,2032,2059]" class="Magnoliopsida" family="Meliaceae" genus="Khaya" kingdom="Plantae" order="Sapindales" pageId="4" pageNumber="69" phylum="Tracheophyta" rank="species" species="senegalensis">
<emphasis id="B944EF3DC84B8B25FBF89B87FB0DF7D4" box="[1058,1279,2032,2058]" italics="true" pageId="4" pageNumber="69">Khaya senegalensis</emphasis>
(Desr.) A. Juss
</taxonomicName>
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.
</paragraph>
<paragraph id="8B8F332FC84A8B24FF529CE9FC93FF02" blockId="5.[136,1451,158,221]" pageId="5" pageNumber="70">
(
<taxonomicName id="4C3048ACC84A8B24FF4A9CE9FEFBFF66" box="[144,265,158,184]" class="Magnoliopsida" family="Meliaceae" kingdom="Plantae" order="Sapindales" pageId="5" pageNumber="70" phylum="Tracheophyta" rank="family">Meliaceae</taxonomicName>
), 
<materialsCitation id="3B583972C84A8B24FEC79CE9FF1BFF02" collectingDate="2021-07-27" collectionCode="ZHKUCC" collectorName="Y. H. Yang &amp; C. F. Liao" pageId="5" pageNumber="70" specimenCode="ZHKUCC 22-0104" specimenCount="1" typeStatus="holotype">
<date id="FF8E15EFC84A8B24FEC79CE9FE46FF66" box="[285,436,158,184]" pageId="5" pageNumber="70" value="2021-07-27">
<collectingDate id="EFCAEC07C84A8B24FEC79CE9FE46FF66" box="[285,436,158,184]" pageId="5" pageNumber="70" value="2021-07-27">27 July 2021</collectingDate>
</date>
, 
<collectorName id="26C556F9C84A8B24FE1A9CE8FDC6FF67" box="[448,564,159,185]" pageId="5" pageNumber="70">Y.H. Yang</collectorName>
&amp; 
<collectorName id="26C556F9C84A8B24FD819CE9FD3AFF66" box="[603,712,158,184]" pageId="5" pageNumber="70">C.F. Liao</collectorName>
, FZL002 (ZHKU 22-0055, 
<emphasis id="B944EF3DC84A8B24FBDC9CE9FB9FFF66" bold="true" box="[1030,1133,158,184]" pageId="5" pageNumber="70">
<typeStatus id="548B8D8DC84A8B24FBDC9CE9FB9FFF66" box="[1030,1133,158,184]" pageId="5" pageNumber="70">holotype</typeStatus>
</emphasis>
), ex-type culture 
<specimenCode id="DB969B54C84A8B24FAE29CE9FF1BFF02" collectionCode="ZHKUCC" pageId="5" pageNumber="70">ZHKUCC 22-0104</specimenCode>
</materialsCitation>
; 
<materialsCitation id="3B583972C84A8B24FF2E9CB5FD88FF03" box="[244,634,194,221]" collectionCode="ZHKUCC" pageId="5" pageNumber="70" specimenCode="ZHKUCC 22-0105" specimenCount="1" typeStatus="holotype">
living cultures 
<specimenCode id="DB969B54C84A8B24FE469CB5FD84FF03" box="[412,630,194,221]" collectionCode="ZHKUCC" pageId="5" pageNumber="70">ZHKUCC 22-0105</specimenCode>
,
</materialsCitation>
<materialsCitation id="3B583972C84A8B24FD589CB5FCAFFF02" box="[642,861,194,220]" collectionCode="ZHKUCC" isTailEnumeration="true" pageId="5" pageNumber="70" specimenCode="ZHKUCC 22-0106" specimenCount="1" typeStatus="holotype">
<specimenCode id="DB969B54C84A8B24FD589CB5FCAFFF02" box="[642,861,194,220]" collectionCode="ZHKUCC" pageId="5" pageNumber="70">ZHKUCC 22-0106</specimenCode>
</materialsCitation>
.
</paragraph>
<caption id="DF4F63A7C84A8B24FF5299CCFEAEF9E3" ID-DOI="http://doi.org/10.5281/zenodo.7989215" ID-Zenodo-Dep="7989215" httpUri="https://zenodo.org/record/7989215/files/figure.png" pageId="5" pageNumber="70" startId="5.[136,229,1467,1489]" targetBox="[178,1436,242,1439]" targetPageId="5" targetType="figure">
<paragraph id="8B8F332FC84A8B24FF5299CCFEAEF9E3" blockId="5.[136,1451,1467,1597]" pageId="5" pageNumber="70">
<emphasis id="B944EF3DC84A8B24FF5299CCFEF2FA0E" bold="true" box="[136,256,1467,1489]" pageId="5" pageNumber="70">FIGURE 1.</emphasis>
Best scoring RAxML tree based on a combined dataset of the LSU, SSU, 
<emphasis id="B944EF3DC84A8B24FC3D99CCFBE9FA0E" box="[999,1051,1467,1488]" italics="true" pageId="5" pageNumber="70">rpb2,</emphasis>
and 
<emphasis id="B944EF3DC84A8B24FB9799CCFB83FA0E" box="[1101,1137,1467,1488]" italics="true" pageId="5" pageNumber="70">tef1</emphasis>
-α partial sequences. The tree is rooted in 
<taxonomicName id="4C3048ACC84A8B24FF3D99A8FE35FA2A" box="[231,455,1503,1524]" class="Dothideomycetes" family="Mytilinidiaceae" genus="Mytilinidion" kingdom="Fungi" order="Mytilinidiales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="andinense">
<emphasis id="B944EF3DC84A8B24FF3D99A8FE35FA2A" box="[231,455,1503,1524]" italics="true" pageId="5" pageNumber="70">Mytilinidion andinense</emphasis>
</taxonomicName>
(CBS 123562) and 
<taxonomicName id="4C3048ACC84A8B24FD549997FCD5FA2A" box="[654,807,1503,1525]" class="Dothideomycetes" family="Mytilinidiaceae" genus="Mytilinidion" kingdom="Fungi" order="Mytilinidiales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="mytilinellum">
<emphasis id="B944EF3DC84A8B24FD549997FCD5FA2A" box="[654,807,1503,1525]" italics="true" pageId="5" pageNumber="70">M. mytilinellum</emphasis>
</taxonomicName>
(CBS 303.34). Bootstrap support values for maximum likelihood (ML) ≥ 75% and Bayesian Probability (BYPP) ≥ 0.95 are indicated above the branches. Ex-type strains are in bold, and newly generated sequences are in blue.
</paragraph>
</caption>
<paragraph id="8B8F332FC84A8B24FF679A1BFD4EF7CC" blockId="5.[136,1452,1644,2066]" pageId="5" pageNumber="70">
<emphasis id="B944EF3DC84A8B24FF679A1BFEFBF958" bold="true" box="[189,265,1644,1670]" pageId="5" pageNumber="70">Notes:</emphasis>
—In the multigene (LSU, SSU, 
<emphasis id="B944EF3DC84A8B24FDA09A1BFD5CF958" box="[634,686,1644,1670]" italics="true" pageId="5" pageNumber="70">rpb2</emphasis>
, and 
<emphasis id="B944EF3DC84A8B24FD359A1BFCEBF958" box="[751,793,1644,1670]" italics="true" pageId="5" pageNumber="70">tef1</emphasis>
-α) phylogenetic analyses of our three isolates (
<collectionCode id="ED21ABEAC84A8B24FAEC9A1BFA59F958" box="[1334,1451,1644,1670]" pageId="5" pageNumber="70">ZHKUCC</collectionCode>
22-0104, 
<collectionCode id="ED21ABEAC84A8B24FF2E9AE7FE95F974" box="[244,359,1680,1706]" pageId="5" pageNumber="70">ZHKUCC</collectionCode>
22-0105, and 
<collectionCode id="ED21ABEAC84A8B24FDD69AE7FD8DF974" box="[524,639,1680,1706]" pageId="5" pageNumber="70">ZHKUCC</collectionCode>
22-0106) formed a sister clade with 
<taxonomicName id="4C3048ACC84A8B24FBF89AE7FB3CF974" box="[1058,1230,1680,1706]" class="Dothideomycetes" family="Massariaceae" genus="Neomassaria" kingdom="Fungi" order="Pleosporales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="hongheensis">
<emphasis id="B944EF3DC84A8B24FBF89AE7FB3CF974" box="[1058,1230,1680,1706]" italics="true" pageId="5" pageNumber="70">N. hongheensis</emphasis>
</taxonomicName>
(
<collectionCode id="ED21ABEAC84A8B24FB049AE7FAB5F974" box="[1246,1351,1680,1706]" pageId="5" pageNumber="70">KUMCC</collectionCode>
21-0340 and 
<collectionCode id="ED21ABEAC84A8B24FF619AC3FED0F910" box="[187,290,1716,1742]" pageId="5" pageNumber="70">KUMCC</collectionCode>
21-0344) and 
<taxonomicName id="4C3048ACC84A8B24FE159AC3FDA8F910" box="[463,602,1716,1742]" class="Dothideomycetes" family="Massariaceae" genus="Neomassaria" kingdom="Fungi" order="Pleosporales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="alstoniae">
<emphasis id="B944EF3DC84A8B24FE159AC3FDA8F910" box="[463,602,1716,1742]" italics="true" pageId="5" pageNumber="70">N. alstoniae</emphasis>
</taxonomicName>
(
<collectingCountry id="F32773BFC84A8B24FDB19AC3FD7CF910" box="[619,654,1716,1742]" name="American Samoa" pageId="5" pageNumber="70">AS</collectingCountry>
14/ 
<collectionCode id="ED21ABEAC84A8B24FD6A9AC3FCDAF910" box="[688,808,1716,1742]" country="Thailand" httpUri="http://grbio.org/cool/gsgf-e23h" name="Mae Fah Luang University Culture Collection" pageId="5" pageNumber="70">MFLUCC</collectionCode>
21-0213). Our isolation 
<taxonomicName id="4C3048ACC84A8B24FB909AC3FB4FF910" box="[1098,1213,1716,1742]" class="Dothideomycetes" family="Massariaceae" genus="Neomassaria" kingdom="Fungi" order="Pleosporales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="khayae">
<emphasis id="B944EF3DC84A8B24FB909AC3FB4FF910" box="[1098,1213,1716,1742]" italics="true" pageId="5" pageNumber="70">N. khayae</emphasis>
</taxonomicName>
have few difference with its closely related taxa by having short papillate, bigger ascomata than 
<taxonomicName id="4C3048ACC84A8B24FC329AAFFB61F92C" box="[1000,1171,1752,1778]" class="Dothideomycetes" family="Massariaceae" genus="Neomassaria" kingdom="Fungi" order="Pleosporales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="hongheensis">
<emphasis id="B944EF3DC84A8B24FC329AAFFB61F92C" box="[1000,1171,1752,1778]" italics="true" pageId="5" pageNumber="70">N. hongheensis</emphasis>
</taxonomicName>
(
<collectionCode id="ED21ABEAC84A8B24FB799AAFFAFEF92C" box="[1187,1292,1752,1778]" pageId="5" pageNumber="70">KUMCC</collectionCode>
21-0340) and 
<taxonomicName id="4C3048ACC84A8B24FF529A8BFEE3F8C8" box="[136,273,1788,1814]" class="Dothideomycetes" family="Massariaceae" genus="Neomassaria" kingdom="Fungi" order="Pleosporales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="alstoniae">
<emphasis id="B944EF3DC84A8B24FF529A8BFEE3F8C8" box="[136,273,1788,1814]" italics="true" pageId="5" pageNumber="70">N. alstoniae</emphasis>
</taxonomicName>
(
<collectingCountry id="F32773BFC84A8B24FEFA9A8BFEB1F8C8" box="[288,323,1788,1814]" name="American Samoa" pageId="5" pageNumber="70">AS</collectingCountry>
14/ 
<collectionCode id="ED21ABEAC84A8B24FEBF9A8BFE2FF8C8" box="[357,477,1788,1814]" country="Thailand" httpUri="http://grbio.org/cool/gsgf-e23h" name="Mae Fah Luang University Culture Collection" pageId="5" pageNumber="70">MFLUCC</collectionCode>
21-0213), thicker peridium than 
<taxonomicName id="4C3048ACC84A8B24FC8D9A8BFBF0F8C8" box="[855,1026,1788,1814]" class="Dothideomycetes" family="Massariaceae" genus="Neomassaria" kingdom="Fungi" order="Pleosporales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="hongheensis">
<emphasis id="B944EF3DC84A8B24FC8D9A8BFBF0F8C8" box="[855,1026,1788,1814]" italics="true" pageId="5" pageNumber="70">N. hongheensis</emphasis>
</taxonomicName>
(
<collectionCode id="ED21ABEAC84A8B24FBC89A8BFB89F8C8" box="[1042,1147,1788,1814]" pageId="5" pageNumber="70">KUMCC</collectionCode>
21-0340) and 
<taxonomicName id="4C3048ACC84A8B24FAF99A8BFA5EF8C8" box="[1315,1452,1788,1814]" class="Dothideomycetes" family="Massariaceae" genus="Neomassaria" kingdom="Fungi" order="Pleosporales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="alstoniae">
<emphasis id="B944EF3DC84A8B24FAF99A8BFA5EF8C8" box="[1315,1452,1788,1814]" italics="true" pageId="5" pageNumber="70">N. alstoniae</emphasis>
</taxonomicName>
(
<collectingCountry id="F32773BFC84A8B24FF4A9B57FF41F8E4" box="[144,179,1824,1850]" name="American Samoa" pageId="5" pageNumber="70">AS</collectingCountry>
14/ 
<collectionCode id="ED21ABEAC84A8B24FF0E9B57FEBEF8E4" box="[212,332,1824,1850]" country="Thailand" httpUri="http://grbio.org/cool/gsgf-e23h" name="Mae Fah Luang University Culture Collection" pageId="5" pageNumber="70">MFLUCC</collectionCode>
21-0213). 
<taxonomicName id="4C3048ACC84A8B24FE029B57FD37F8E4" box="[472,709,1824,1850]" class="Dothideomycetes" family="Massariaceae" genus="Neomassaria" kingdom="Fungi" order="Pleosporales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="khayae">
<emphasis id="B944EF3DC84A8B24FE029B57FD37F8E4" box="[472,709,1824,1850]" italics="true" pageId="5" pageNumber="70">Neomassaria khayae</emphasis>
</taxonomicName>
develop ascospores which are 1–2-septate, longer and narrower than 
<taxonomicName id="4C3048ACC84A8B24FF199B33FE83F880" box="[195,369,1860,1886]" class="Dothideomycetes" family="Massariaceae" genus="Neomassaria" kingdom="Fungi" order="Pleosporales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="hongheensis">
<emphasis id="B944EF3DC84A8B24FF199B33FE83F880" box="[195,369,1860,1886]" italics="true" pageId="5" pageNumber="70">N. hongheensis</emphasis>
</taxonomicName>
(
<collectionCode id="ED21ABEAC84A8B24FE5E9B33FE1FF880" box="[388,493,1860,1886]" pageId="5" pageNumber="70">KUMCC</collectionCode>
21-0340) and 
<taxonomicName id="4C3048ACC84A8B24FD469B33FCD5F880" box="[668,807,1860,1886]" class="Dothideomycetes" family="Massariaceae" genus="Neomassaria" kingdom="Fungi" order="Pleosporales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="alstoniae">
<emphasis id="B944EF3DC84A8B24FD469B33FCD5F880" box="[668,807,1860,1886]" italics="true" pageId="5" pageNumber="70">N. alstoniae</emphasis>
</taxonomicName>
(
<collectingCountry id="F32773BFC84A8B24FCE39B33FCAEF880" box="[825,860,1860,1886]" name="American Samoa" pageId="5" pageNumber="70">AS</collectingCountry>
14/ 
<collectionCode id="ED21ABEAC84A8B24FCA49B33FC04F880" box="[894,1014,1860,1886]" country="Thailand" httpUri="http://grbio.org/cool/gsgf-e23h" name="Mae Fah Luang University Culture Collection" pageId="5" pageNumber="70">MFLUCC</collectionCode>
21-0213). In addition, morphological profiles of all 
<taxonomicName id="4C3048ACC84A8B24FEF39B1FFDE4F85C" box="[297,534,1896,1922]" pageId="5" pageNumber="70">
<emphasis id="B944EF3DC84A8B24FEF39B1FFE4FF85F" box="[297,445,1896,1921]" italics="true" pageId="5" pageNumber="70">Neomassaria</emphasis>
species
</taxonomicName>
are provided in 
<tableCitation id="C6B20694C84A8B24FD0B9B1FFCB2F85C" box="[721,832,1896,1922]" captionStart="TABLE 1" captionStartId="2.[136,224,628,652]" captionText="TABLE 1. Genes/loci used in the study with primers, protocols and references." pageId="5" pageNumber="70">TABLE 1</tableCitation>
. The nucleotide differences between 
<taxonomicName id="4C3048ACC84A8B24FB3D9B1FFAAAF85C" box="[1255,1368,1896,1922]" class="Dothideomycetes" family="Massariaceae" genus="Neomassaria" kingdom="Fungi" order="Pleosporales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="khayae">
<emphasis id="B944EF3DC84A8B24FB3D9B1FFAAAF85C" box="[1255,1368,1896,1922]" italics="true" pageId="5" pageNumber="70">N. khayae</emphasis>
</taxonomicName>
and its phylogenetically related species are as follows; 
<taxonomicName id="4C3048ACC84A8B24FD409BFBFCB6F878" box="[666,836,1932,1958]" class="Dothideomycetes" family="Massariaceae" genus="Neomassaria" kingdom="Fungi" order="Pleosporales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="hongheensis">
<emphasis id="B944EF3DC84A8B24FD409BFBFCB6F878" box="[666,836,1932,1958]" italics="true" pageId="5" pageNumber="70">N. hongheensis</emphasis>
</taxonomicName>
(
<collectionCode id="ED21ABEAC84A8B24FC889BFBFC49F878" box="[850,955,1932,1958]" pageId="5" pageNumber="70">KUMCC</collectionCode>
21-0344) LSU: 2.24% (19/847 bases), SSU: 0.88% (9/1019 bases), 
<emphasis id="B944EF3DC84A8B24FE499BC7FE3AF814" box="[403,456,1968,1994]" italics="true" pageId="5" pageNumber="70">rpb2</emphasis>
: 12.33% (128/1038), 
<emphasis id="B944EF3DC84A8B24FD129BC7FD00F814" box="[712,754,1968,1994]" italics="true" pageId="5" pageNumber="70">tef1</emphasis>
-α: 5.58% (50/896); 
<taxonomicName id="4C3048ACC84A8B24FC389BC7FB9EF814" box="[994,1132,1968,1994]" class="Dothideomycetes" family="Massariaceae" genus="Neomassaria" kingdom="Fungi" order="Pleosporales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="alstoniae">
<emphasis id="B944EF3DC84A8B24FC389BC7FB9EF814" box="[994,1132,1968,1994]" italics="true" pageId="5" pageNumber="70">N. alstoniae</emphasis>
</taxonomicName>
(
<collectingCountry id="F32773BFC84A8B24FBA49BC7FB53F814" box="[1150,1185,1968,1994]" name="American Samoa" pageId="5" pageNumber="70">AS</collectingCountry>
14/ 
<collectionCode id="ED21ABEAC84A8B24FB199BC7FAC9F814" box="[1219,1339,1968,1994]" country="Thailand" httpUri="http://grbio.org/cool/gsgf-e23h" name="Mae Fah Luang University Culture Collection" pageId="5" pageNumber="70">MFLUCC</collectionCode>
21-0213) LSU: 1.12% (17/846 bases), SSU: 1.21% (12/988 bases). Therefore, based on both morphological and phylogenetic evidence, 
<taxonomicName id="4C3048ACC84A8B24FF209B8FFE9BF7CC" box="[250,361,2040,2066]" class="Dothideomycetes" family="Massariaceae" genus="Neomassaria" kingdom="Fungi" order="Pleosporales" pageId="5" pageNumber="70" phylum="Ascomycota" rank="species" species="khayae">
<emphasis id="B944EF3DC84A8B24FF209B8FFE9BF7CC" box="[250,361,2040,2066]" italics="true" pageId="5" pageNumber="70">N. khayae</emphasis>
</taxonomicName>
is described as a new species.
</paragraph>
</subSubSection>
</treatment>
</document>