355 lines
42 KiB
XML
355 lines
42 KiB
XML
<document id="C82D54C4ABBD7F97369BCF1966F9D0A3" ID-DOI="10.11646/phytotaxa.630.1.3" ID-ISSN="1179-3163" ID-Zenodo-Dep="10361447" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="juliana" IM.materialsCitations_approvedBy="juliana" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="felipe" IM.treatmentCitations_approvedBy="felipe" IM.treatments_approvedBy="juliana" checkinTime="1702374014946" checkinUser="plazi" docAuthor="Brahmanage, Rashika S., Wijayawardene, Nalin N., Nanayakkara, Chandrika M., Muthumala, Chaminda K., Wijesundara, Siril, Dai, Dong Q. & Ariyawansa, Kahandawa G. S. U." docDate="2023" docId="6B13D3691006D1709FDAFC23221FFE63" docLanguage="en" docName="phytotaxa.630.1.3.pdf" docOrigin="Phytotaxa 630 (1)" docSource="https://phytotaxa.mapress.com/pt/article/download/phytotaxa.630.1.3/51355" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.13:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="13" docTitle="Coniella eucalyptorum L. V. Alvarez & Crous, Stud. Mycol." docType="treatment" docVersion="3" lastPageNumber="45" masterDocId="972AAB111005D1759F52FF97264BF906" masterDocTitle="Eucalyptus leaf spot disease caused by Coniella eucalyptorum in Sri Lanka" masterLastPageNumber="50" masterPageNumber="40" pageNumber="43" updateTime="1702555443318" updateUser="juliana" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
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<mods:titleInfo id="96C4ABE3EC20279C13D3E45AFFA31442">
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<mods:title id="79DD64147E67CAD9F58ECC8F8A00CA49">Eucalyptus leaf spot disease caused by Coniella eucalyptorum in Sri Lanka</mods:title>
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<mods:namePart id="5C584248DC3D9FBCD4A67A066659F1AE">Brahmanage, Rashika S.</mods:namePart>
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<mods:nameIdentifier id="F659E510C0B1802E369D276425BB87D0" type="ORCID">0000-0002-7846-5890</mods:nameIdentifier>
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<mods:affiliation id="E2EBAE1E573122E9AF00B1119F258CE0">Center for Yunnan Plateau Biological Resources Protection and Utilization, Qujing Normal University, Qujing, Yunnan Province, 655011 P. R. China. & Department of Plant Sciences, Faculty of Science, University of Colombo, Colombo 00700, Sri Lanka. & National Institute of Fundamental Studies, Hanthana, Kandy, 20000, Sri Lanka. & Tropical Microbiology Research Foundation, 96 / N / 10, Meemanagoda Road, 10230 Pannipitiya, Sri Lanka.</mods:affiliation>
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<mods:nameIdentifier id="5B5BF821801FEA7857DE5CC75ACD4515" type="email">sajith2007@gmail.com</mods:nameIdentifier>
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<mods:namePart id="724E26B7CC3504018AE2A58B6F28BC45">Wijayawardene, Nalin N.</mods:namePart>
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<mods:nameIdentifier id="F27AE2290332E070DBA748D7568CD48B" type="ORCID">0000-0003-0522-5498</mods:nameIdentifier>
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<mods:affiliation id="E81EE10E58E12D5934658D3B21F18087">Center for Yunnan Plateau Biological Resources Protection and Utilization, Qujing Normal University, Qujing, Yunnan Province, 655011 P. R. China. & Tropical Microbiology Research Foundation, 96 / N / 10, Meemanagoda Road, 10230 Pannipitiya, Sri Lanka.</mods:affiliation>
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<mods:nameIdentifier id="F4C4AADD73705CE9FE73AB1F0D6EE0F7" type="email">nalinwijayawardene@yahoo.com</mods:nameIdentifier>
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<mods:namePart id="F08A4CF25363AE7BE50761DDC921D062">Nanayakkara, Chandrika M.</mods:namePart>
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<mods:nameIdentifier id="53AAAA91FDFA19452009F4A733A9565E" type="ORCID">0000-0002-4683-9288</mods:nameIdentifier>
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<mods:affiliation id="A4B4A63AB15AB20440E9C0C7857767CD">Department of Plant Sciences, Faculty of Science, University of Colombo, Colombo 00700, Sri Lanka.</mods:affiliation>
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<mods:nameIdentifier id="189B03F0F517325A8B0298A6A984D6A1" type="email">chandi@pts.cmb.ac.lk</mods:nameIdentifier>
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<mods:namePart id="2AA9D894F8E7792590227D3A5774D819">Muthumala, Chaminda K.</mods:namePart>
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<mods:nameIdentifier id="6C95A396868D52A40D7B1F787067F486" type="ORCID">0000-0001-9358-7717</mods:nameIdentifier>
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<mods:affiliation id="FEB1BCC2DA5FFDDE58FED86534390E9E">State Timber Corporation Sri Lanka, No. 82, “ Sampath Paya ”, Rajamalwatte Road, Sri Jayawardenepura Kotte, 10100 Sri Lanka.</mods:affiliation>
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<mods:nameIdentifier id="94041608283D1D6FD916E20F2CAB6692" type="email">ck_muthumala@yahoo.com</mods:nameIdentifier>
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<mods:namePart id="7BDD6C7E9DA92D4790BE208137425632">Wijesundara, Siril</mods:namePart>
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<mods:nameIdentifier id="79283B8C95C0C9FA376CFD60A06E4773" type="ORCID">0000-0002-6754-8201</mods:nameIdentifier>
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<mods:affiliation id="E7AB23128E9438D1608D0A7E25274BAB">National Institute of Fundamental Studies, Hanthana, Kandy, 20000, Sri Lanka.</mods:affiliation>
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<mods:nameIdentifier id="24693AA7087D0B6F47BE44F316EC4536" type="email">siril.wi@nifs.ac.lk</mods:nameIdentifier>
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<mods:namePart id="38E3FAD2007E5FEC2F6A2117EC3192E3">Dai, Dong Q.</mods:namePart>
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<mods:nameIdentifier id="A59A838F90E6C185DC019F8969FE5CDE" type="ORCID">0000-0001-8935-8807</mods:nameIdentifier>
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<mods:affiliation id="F617B41D54FED62B616170C857CEF77B">Center for Yunnan Plateau Biological Resources Protection and Utilization, Qujing Normal University, Qujing, Yunnan Province, 655011 P. R. China.</mods:affiliation>
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<mods:nameIdentifier id="65E21F68D0D0D4B4782944368CBDBD3D" type="email">cicidaidongqin@gmail.com</mods:nameIdentifier>
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</mods:name>
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<mods:name id="52F38823FA80119828E94E394014AEA0" type="personal">
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</mods:role>
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<mods:namePart id="EA718E97E035EF10DE86F48E3404E5B0">Ariyawansa, Kahandawa G. S. U.</mods:namePart>
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<mods:nameIdentifier id="6A9149000CCFBB8E0204B27AD74BBDE8" type="ORCID">0000-0002-5485-6053</mods:nameIdentifier>
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<mods:affiliation id="A070A5CD3C7E695E5676307B25DD0B94">Department of Plant Sciences, Faculty of Science, University of Colombo, Colombo 00700, Sri Lanka.</mods:affiliation>
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<mods:nameIdentifier id="B886191F2ACB3F326552A05ACADBC96B" type="email">sameera@pts.cmb.ac.lk</mods:nameIdentifier>
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<mods:typeOfResource id="68655FB8CA660A4FC0502BBB6AFACCCE">text</mods:typeOfResource>
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<mods:title id="C55F2E59BE04FC47645F35EE7CA370A5">Phytotaxa</mods:title>
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</mods:titleInfo>
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<mods:part id="5D461500ACBA6326E49A3F659DFFDC1E">
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<mods:date id="DB05AD9341229D864ACEFE6CD4CB7767">2023</mods:date>
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<mods:number id="85935E26E8D59636B5213DE7CE855776">2023-12-12</mods:number>
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<mods:detail id="BBB46F80288D0138E95F833BFE5F6155" type="volume">
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<mods:number id="1A12D98FEBE4378C279434B647A0ECDD">630</mods:number>
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<mods:number id="D6FE073A3DBAC52F45EA967156231FAD">1</mods:number>
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<mods:start id="AE7F7B39D474AB981BCE7622E729B154">40</mods:start>
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<mods:end id="84B88447F6A8CD6B213B0AC7F1A7B3B1">50</mods:end>
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<mods:url id="85E02FE020C637D49A49D5369C5FCC6F">https://phytotaxa.mapress.com/pt/article/download/phytotaxa.630.1.3/51355</mods:url>
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<mods:classification id="28B7492BA95019B2DFB4CFA66C08B96B">journal article</mods:classification>
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<mods:identifier id="134BA8A4652E15CF0212E2D7F41DBE2C" type="DOI">10.11646/phytotaxa.630.1.3</mods:identifier>
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<mods:identifier id="986A690DF89D89432DDF49DFBBD284D7" type="ISSN">1179-3163</mods:identifier>
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<mods:identifier id="4EE0EB7A9B4F402F099715CC1FB7A116" type="Zenodo-Dep">10361447</mods:identifier>
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</mods:mods>
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<treatment id="6B13D3691006D1709FDAFC23221FFE63" LSID="urn:lsid:plazi:treatment:6B13D3691006D1709FDAFC23221FFE63" httpUri="http://treatment.plazi.org/id/6B13D3691006D1709FDAFC23221FFE63" lastPageId="5" lastPageNumber="45" pageId="3" pageNumber="43">
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<subSubSection id="ABA031F41006D1769FDAFC232287FAC8" box="[136,1228,948,975]" pageId="3" pageNumber="43" type="nomenclature">
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<paragraph id="E305627F1006D1769FDAFC232287FAC8" blockId="3.[136,1228,948,975]" box="[136,1228,948,975]" pageId="3" pageNumber="43">
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<heading id="B84DD5131006D1769FDAFC232287FAC8" box="[136,1228,948,975]" fontSize="11" level="2" pageId="3" pageNumber="43" reason="3">
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<treatmentCitation id="621B446E1006D1769FDAFC232287FAC8" author="L. V. Alvarez & Crous" box="[136,1228,948,975]" journal="Stud. Mycol." page="15" pageId="3" pageNumber="43" score="0.005251091943919118" volume="85" year="2016">
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<taxonomicName id="24BA19FC1006D1769FDAFC232266FAC8" ID-CoL="XM49" authority="(Crous & M. J. Wingf.) L. V. Alvarez & Crous, Stud. Mycol." authorityName="L. V. Alvarez & Crous, Stud. Mycol." baseAuthorityName="Crous & M. J. Wingf." box="[136,1069,948,975]" class="Sordariomycetes" family="Schizoparmaceae" genus="Coniella" kingdom="Fungi" order="Diaporthales" pageId="3" pageNumber="43" phylum="Ascomycota" rank="species" species="eucalyptorum">
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<emphasis id="D1CEBE6D1006D1769FDAFC2327DBFAC9" bold="true" box="[136,400,948,975]" italics="true" pageId="3" pageNumber="43">Coniella eucalyptorum</emphasis>
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(Crous & M.J. Wingf.) L.V. Alvarez & Crous,
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<emphasis id="D1CEBE6D1006D1769CCDFC232266FAC8" box="[927,1069,948,974]" italics="true" pageId="3" pageNumber="43">Stud. Mycol.</emphasis>
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</taxonomicName>
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||
<emphasis id="D1CEBE6D1006D1769B61FC232204FAC8" bold="true" box="[1075,1103,948,974]" pageId="3" pageNumber="43">85</emphasis>
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: 15 (2016)
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</treatmentCitation>
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</heading>
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</paragraph>
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||
</subSubSection>
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<subSubSection id="ABA031F41006D1769FDAFC4F24A6FAF4" box="[136,749,984,1011]" pageId="3" pageNumber="43" type="description">
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<paragraph id="E305627F1006D1769FDAFC4F24A6FAF4" blockId="3.[136,749,984,1011]" box="[136,749,984,1011]" pageId="3" pageNumber="43">
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Index Fungorum Registration Identifier 817817,
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<figureCitation id="7B817EFA1006D1769DFEFC4F24A6FAF4" box="[684,749,984,1010]" captionStart="FIGURE 2" captionStartId="6.[136,229,982,1004]" captionTargetBox="[303,1287,191,950]" captionTargetId="figure-18@6.[295,1293,187,955]" captionTargetPageId="6" captionText="FIGURE 2. Coniella eucalyptorum (NIFSMC-RB-LP31) a–b Disease symptoms on the leaves of Eucalyptus camaldulensis. c–d Appearance of Conidiomata on E. camaldulensis. e Wall of conidiomata. f Conidiogenous cell. g–i Conidia. j Above view of the culture on PDA k Reverse view of the culture e = 50 μm, f–i = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.10361451" httpUri="https://zenodo.org/record/10361451/files/figure.png" pageId="3" pageNumber="43">Fig. 2</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection id="ABA031F41006D1769FDAFBB32335FD3F" box="[136,1406,1060,1082]" pageId="3" pageNumber="43" type="reference_group">
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<paragraph id="E305627F1006D1769FDAFBB32335FD3F" blockId="3.[136,1406,1060,1082]" box="[136,1406,1060,1082]" pageId="3" pageNumber="43">
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Basionym:
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<taxonomicName id="24BA19FC1006D1769FA4FBB324E6FD3C" ID-CoL="6VKJD" authority="Crous & M. J. Wingf." authorityName="Crous & M. J. Wingf." box="[246,685,1060,1082]" class="Sordariomycetes" family="Schizoparmaceae" genus="Pilidiella" kingdom="Fungi" order="Diaporthales" pageId="3" pageNumber="43" phylum="Ascomycota" rank="species" species="eucalyptorum">
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<emphasis id="D1CEBE6D1006D1769FA4FBB32792FD3F" box="[246,473,1060,1081]" italics="true" pageId="3" pageNumber="43">Pilidiella eucalyptorum</emphasis>
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Crous & M.J. Wingf.
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</taxonomicName>
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, in Van Niekerk, Groenewald, Verkley, Fourie, Wingfield & Crous, (2004).
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</paragraph>
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</subSubSection>
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<subSubSection id="ABA031F41006D1769FDAFBFF23DBFCA4" pageId="3" pageNumber="43" type="description">
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<paragraph id="E305627F1006D1769FDAFBFF26A3FC5C" blockId="3.[136,1452,1128,1910]" pageId="3" pageNumber="43">
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<emphasis id="D1CEBE6D1006D1769FDAFBFF2743FD84" box="[136,264,1128,1154]" italics="true" pageId="3" pageNumber="43">Pathogenic</emphasis>
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on leaves of
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<taxonomicName id="24BA19FC1006D1769ECEFBFE2410FD84" ID-CoL="3BPQQ" box="[412,603,1128,1154]" class="Magnoliopsida" family="Myrtaceae" genus="Eucalyptus" kingdom="Plantae" order="Myrtales" pageId="3" pageNumber="43" phylum="Tracheophyta" rank="species" species="camaldulensis">
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<emphasis id="D1CEBE6D1006D1769ECEFBFE2410FD84" box="[412,603,1128,1154]" italics="true" pageId="3" pageNumber="43">E. camaldulensis</emphasis>
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</taxonomicName>
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.
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<emphasis id="D1CEBE6D1006D1769D3BFBFF255BFD84" bold="true" box="[617,784,1128,1154]" pageId="3" pageNumber="43">Sexual morph</emphasis>
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: Undetermined.
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<emphasis id="D1CEBE6D1006D1769C9EFBFF22C8FD84" bold="true" box="[972,1155,1128,1154]" pageId="3" pageNumber="43">Asexual morph</emphasis>
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:
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<emphasis id="D1CEBE6D1006D1769BC3FBFF2368FD84" box="[1169,1315,1128,1154]" italics="true" pageId="3" pageNumber="43">Conidiomata</emphasis>
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80−140 μm long, 150−200 μm in diameter (n= 15), solitary or aggregated, globose, brown, and dark brown to black from the top.
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<emphasis id="D1CEBE6D1006D1769FDAFB272771FDCC" box="[136,314,1200,1226]" italics="true" pageId="3" pageNumber="43">Conidioma wall</emphasis>
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consisting of 2–3 layers of hyaline
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<emphasis id="D1CEBE6D1006D1769D96FB2625DAFDCC" box="[708,913,1201,1226]" italics="true" pageId="3" pageNumber="43">textura prismatica</emphasis>
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and 4−5 layers of brown
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<emphasis id="D1CEBE6D1006D1769BF8FB262320FDCC" box="[1194,1387,1200,1226]" italics="true" pageId="3" pageNumber="43">textura angularis</emphasis>
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cells.
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<emphasis id="D1CEBE6D1006D1769FDAFB432726FDE8" box="[136,365,1236,1262]" italics="true" pageId="3" pageNumber="43">Conidiogenous cells</emphasis>
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12–15 × 1–2 μm (x̄ = 13 × 2 μm, n = 20), annellidic, narrowing at the tip, smooth and hyaline.
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<taxonomicName id="24BA19FC1006D1769FDAFB6F26A9FC14" box="[136,226,1272,1298]" class="Arthoniomycetes" family="Arthoniaceae" genus="Conidia" higherTaxonomySource="GBIF" kingdom="Fungi" order="Arthoniales" pageId="3" pageNumber="43" phylum="Ascomycota" rank="genus">
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<emphasis id="D1CEBE6D1006D1769FDAFB6F26A9FC14" box="[136,226,1272,1298]" italics="true" pageId="3" pageNumber="43">Conidia</emphasis>
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</taxonomicName>
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10−13 × 3−6 μm (x̄ = 12 × 5 μm, n = 30), hyaline to pale brown, becoming dark brown at maturity, smooth, broadly ellipsoidal, both sides gradually tapering, smooth-walled, and multi-guttulate with one or two prominent guttules.
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</paragraph>
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<paragraph id="E305627F1006D1769FEFFAF323DBFCA4" blockId="3.[136,1452,1128,1910]" pageId="3" pageNumber="43">
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Culture characteristics: Colony on PDA reaching
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<quantity id="2442CF9A1006D1769DA9FAF32506FC79" box="[763,845,1380,1407]" metricMagnitude="-2" metricUnit="m" metricValue="2.5" pageId="3" pageNumber="43" unit="mm" value="25.0">25 mm</quantity>
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diameter after seven days at 25 °C, colony circular, margin wavy, flat, velvety appearance, colony from above: pale pink to brown; reverse: light brown to dark brown.
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</paragraph>
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</subSubSection>
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<subSubSection id="ABA031F41006D1769FEFFA3B22DEFF34" pageId="3" pageNumber="43" type="materials_examined">
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<paragraph id="E305627F1006D1769FEFFA3B2794FF08" blockId="3.[136,1452,1128,1910]" pageId="3" pageNumber="43">
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Material examined:
|
||
<materialsCitation id="53D268221006D1769EF2FA3B2792FF08" accessionNumbers="OR141147, OR143784, OR461287" collectingDate="2022-05-05" collectionCode="NIFSCC" collectorName="Rashika S. Brahmanage" country="Sri Lanka" location="Aralaganwila" municipality="Polonnaruwa District" pageId="3" pageNumber="43" specimenCode="NIFSCC- RB-LP31, UOCCC-RB-LP31" specimenCount="1" stateProvince="North Central Province">
|
||
<collectingCountry id="9BAD22EF1006D1769EF2FA3B245AFCC0" box="[416,529,1452,1478]" name="Sri Lanka" pageId="3" pageNumber="43">Sri Lanka</collectingCountry>
|
||
,
|
||
<collectingRegion id="217EAC9D1006D1769D4FFA3B256DFCC0" box="[541,806,1452,1478]" country="Sri Lanka" name="North Central" pageId="3" pageNumber="43">North Central Province</collectingRegion>
|
||
,
|
||
<collectingMunicipality id="0361F8051006D1769C60FA3B226EFCC0" box="[818,1061,1452,1478]" pageId="3" pageNumber="43">Polonnaruwa District</collectingMunicipality>
|
||
,
|
||
<location id="E66534A41006D1769B7DFA3B2282FCC0" LSID="urn:lsid:plazi:treatment:6B13D3691006D1709FDAFC23221FFE63:E66534A41006D1769B7DFA3B2282FCC0" box="[1071,1225,1452,1478]" country="Sri Lanka" municipality="Polonnaruwa District" name="Aralaganwila" pageId="3" pageNumber="43" stateProvince="North Central Province">Aralaganwila</location>
|
||
, (Latitude 7.293889 Longitude 81.2109594) on leaves of
|
||
<taxonomicName id="24BA19FC1006D1769D72FA462495FCEC" box="[544,734,1488,1514]" class="Magnoliopsida" family="Myrtaceae" genus="Eucalyptus" kingdom="Plantae" order="Myrtales" pageId="3" pageNumber="43" phylum="Tracheophyta" rank="species" species="camaldulensis">
|
||
<emphasis id="D1CEBE6D1006D1769D72FA462495FCEC" box="[544,734,1488,1514]" italics="true" pageId="3" pageNumber="43">E. camaldulensis</emphasis>
|
||
</taxonomicName>
|
||
,
|
||
<date id="970444BF1006D1769DB9FA4725D6FCEC" box="[747,925,1488,1515]" pageId="3" pageNumber="43" value="2022-05-05">
|
||
<collectingDate id="8740BD571006D1769DB9FA4725D6FCEC" box="[747,925,1488,1515]" pageId="3" pageNumber="43">
|
||
5
|
||
<superScript id="14CFCF371006D1769DABFA47254DFCD8" attach="left" box="[761,774,1488,1502]" fontSize="6" pageId="3" pageNumber="43">th</superScript>
|
||
of May 2022
|
||
</collectingDate>
|
||
</date>
|
||
,
|
||
<collectorName id="4E4F07A91006D1769CF5FA4722FFFCEC" box="[935,1204,1488,1514]" pageId="3" pageNumber="43">Rashika S. Brahmanage</collectorName>
|
||
, RB-LP31, (
|
||
<specimenCode id="B31CCA041006D1769A6CFA4726BDFF08" pageId="3" pageNumber="43">NIFSCC-RB-LP31</specimenCode>
|
||
,
|
||
<specimenCode id="B31CCA041006D1769E53FA632792FF08" box="[257,473,1524,1550]" pageId="3" pageNumber="43">UOCCC-RB-LP31</specimenCode>
|
||
</materialsCitation>
|
||
)
|
||
</paragraph>
|
||
<paragraph id="E305627F1006D1769FEFF98F22DEFF34" blockId="3.[136,1452,1128,1910]" box="[189,1173,1560,1587]" pageId="3" pageNumber="43">
|
||
<materialsCitation id="53D268221006D1769FEFF98F22DEFF34" box="[189,1173,1560,1587]" pageId="3" pageNumber="43" specimenCount="1">
|
||
GenBank accession numbers: ITS =
|
||
<accessionNumber id="FCE9FF9C1006D1769D05F98F249EFF34" box="[599,725,1560,1586]" pageId="3" pageNumber="43">OR141147</accessionNumber>
|
||
, LSU =
|
||
<accessionNumber id="FCE9FF9C1006D1769C60F98F25E4FF34" box="[818,943,1560,1586]" pageId="3" pageNumber="43">OR143784</accessionNumber>
|
||
,
|
||
<emphasis id="D1CEBE6D1006D1769CE9F98F25B6FF34" box="[955,1021,1560,1586]" italics="true" pageId="3" pageNumber="43">tef1-α</emphasis>
|
||
=
|
||
<accessionNumber id="FCE9FF9C1006D1769B49F98F22DEFF34" box="[1051,1173,1560,1586]" pageId="3" pageNumber="43">OR461287</accessionNumber>
|
||
</materialsCitation>
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="ABA031F41006D1709FEFF9AA221FFE63" lastPageId="5" lastPageNumber="45" pageId="3" pageNumber="43" type="discussion">
|
||
<paragraph id="E305627F1006D1769FEFF9AA241EFE70" blockId="3.[136,1452,1128,1910]" pageId="3" pageNumber="43">
|
||
Notes: Based on the morphological characteristics, our strain (NIFSMC-RB-LP31) is similar to the
|
||
<typeStatus id="3C01DCDD1006D1769A1BF9AB23E0FF50" box="[1353,1451,1596,1622]" pageId="3" pageNumber="43" type="holotype">holotype</typeStatus>
|
||
of
|
||
<taxonomicName id="24BA19FC1006D1769FF8F9F727E5FF7C" box="[170,430,1632,1658]" class="Sordariomycetes" family="Schizoparmaceae" genus="Coniella" kingdom="Fungi" order="Diaporthales" pageId="3" pageNumber="43" phylum="Ascomycota" rank="species" species="eucalyptorum">
|
||
<emphasis id="D1CEBE6D1006D1769FF8F9F727E5FF7C" box="[170,430,1632,1658]" italics="true" pageId="3" pageNumber="43">Coniella eucalyptorum</emphasis>
|
||
</taxonomicName>
|
||
(CBS 112640) collected from the leaves of
|
||
<taxonomicName id="24BA19FC1006D1769CE7F9F722D9FF7C" box="[949,1170,1632,1658]" class="Magnoliopsida" family="Myrtaceae" genus="Eucalyptus" kingdom="Plantae" order="Myrtales" pageId="3" pageNumber="43" phylum="Tracheophyta" rank="species" species="grandis">
|
||
<emphasis id="D1CEBE6D1006D1769CE7F9F722D9FF7C" box="[949,1170,1632,1658]" italics="true" pageId="3" pageNumber="43">Eucalyptus grandis</emphasis>
|
||
</taxonomicName>
|
||
×
|
||
<taxonomicName id="24BA19FC1006D1769BE4F9F62313FF7C" box="[1206,1368,1633,1659]" class="Magnoliopsida" family="Myrtaceae" genus="Eucalyptus" kingdom="Plantae" order="Myrtales" pageId="3" pageNumber="43" phylum="Tracheophyta" rank="species" species="tereticornis">
|
||
<emphasis id="D1CEBE6D1006D1769BE4F9F6228CFF7C" box="[1206,1223,1633,1658]" italics="true" pageId="3" pageNumber="43">E</emphasis>
|
||
.
|
||
<emphasis id="D1CEBE6D1006D1769B8AF9F62313FF7C" box="[1240,1368,1633,1658]" italics="true" pageId="3" pageNumber="43">tereticornis</emphasis>
|
||
</taxonomicName>
|
||
hybrid (
|
||
<bibRefCitation id="872B1F8E1006D1769FC2F913273DFF98" author="Alvarez, L. V. & Groenewald, J. Z. & Crous, P. W." box="[144,374,1668,1695]" pageId="3" pageNumber="43" pagination="1 - 34" refId="ref5029" refString="Alvarez, L. V., Groenewald, J. Z. & Crous, P. W. (2016) Revising the Schizoparmaceae: Coniella and its synonyms Pilidiella and Schizoparme. Studies in Mycology 85: 1 - 34. https: // doi. org / 10.1016 / j. simyco. 2016.09.001" type="journal article" year="2016">
|
||
Alvarez
|
||
<emphasis id="D1CEBE6D1006D1769FA6F9122763FF98" box="[244,296,1668,1694]" italics="true" pageId="3" pageNumber="43">et al</emphasis>
|
||
. 2016
|
||
</bibRefCitation>
|
||
). According to multi-gene phylogeny (ITS, LSU, and
|
||
<emphasis id="D1CEBE6D1006D1769CBAF9132261FF98" box="[1000,1066,1668,1694]" italics="true" pageId="3" pageNumber="43">tef1-α</emphasis>
|
||
), our strain (NIFSMC-RB-LP31) clustered with other strains of
|
||
<taxonomicName id="24BA19FC1006D1769E8DF93F24D0FFC4" box="[479,667,1704,1731]" class="Sordariomycetes" family="Schizoparmaceae" genus="Coniella" kingdom="Fungi" order="Diaporthales" pageId="3" pageNumber="43" phylum="Ascomycota" rank="species" species="eucalyptorum">
|
||
<emphasis id="D1CEBE6D1006D1769E8DF93F27B9FFC4" box="[479,498,1704,1730]" italics="true" pageId="3" pageNumber="43">C</emphasis>
|
||
.
|
||
<emphasis id="D1CEBE6D1006D1769D53F93F24D0FFC4" box="[513,667,1704,1730]" italics="true" pageId="3" pageNumber="43">eucalyptorum</emphasis>
|
||
</taxonomicName>
|
||
(CBS 111023, CBS 112640, CBS 114134, and MFLU 17-0675) with 100% ML and 1.00 PP bootstrap support (
|
||
<figureCitation id="7B817EFA1006D1769D38F95B24E6FFE0" box="[618,685,1740,1766]" captionStart="FIGURE 1" captionStartId="4.[136,229,1919,1941]" captionTargetBox="[298,1288,187,1892]" captionTargetId="figure-18@4.[298,1289,187,1892]" captionTargetPageId="4" captionText="FIGURE 1. Phylogram generated for Coniella species from ML and BI analyses based on a combined dataset of ITS, LSU, and tef1-α sequences. The tree is rooted with Melanconiella sp. (CBS110385). Bootstrap support values for ML ≥ 65 % and Bayesian posterior probabilities (PP) ≥ 0.95 are shown at the nodes. The strain from the current study is in red. All ex-type strains are in bold black. Some branches were shortened to fit them to the pages, and these are indicated by two diagonal lines with the number of times a branch was shortened indicated next to the lines." figureDoi="http://doi.org/10.5281/zenodo.10361449" httpUri="https://zenodo.org/record/10361449/files/figure.png" pageId="3" pageNumber="43">Fig. 1</figureCitation>
|
||
). When comparing the ITS, LSU, and
|
||
<emphasis id="D1CEBE6D1006D1769B39F95B22E6FFE0" box="[1131,1197,1740,1766]" italics="true" pageId="3" pageNumber="43">tef1-α</emphasis>
|
||
gene regions, no base pair (0%) differences were observed between our strain (NIFSMC-RB-LP31) and the
|
||
<typeStatus id="3C01DCDD1006D1769B31F967228EFE0C" box="[1123,1221,1776,1802]" pageId="3" pageNumber="43" type="holotype">holotype</typeStatus>
|
||
of
|
||
<taxonomicName id="24BA19FC1006D1769BBDF96723E0FE0C" box="[1263,1451,1776,1802]" class="Sordariomycetes" family="Schizoparmaceae" genus="Coniella" kingdom="Fungi" order="Diaporthales" pageId="3" pageNumber="43" phylum="Ascomycota" rank="species" species="eucalyptorum">
|
||
<emphasis id="D1CEBE6D1006D1769BBDF96723E0FE0C" box="[1263,1451,1776,1802]" italics="true" pageId="3" pageNumber="43">C. eucalyptorum</emphasis>
|
||
</taxonomicName>
|
||
(CBS 112640).
|
||
<taxonomicName id="24BA19FC1006D1769E10F8832403FE28" box="[322,584,1812,1838]" class="Sordariomycetes" family="Schizoparmaceae" genus="Coniella" kingdom="Fungi" order="Diaporthales" pageId="3" pageNumber="43" phylum="Ascomycota" rank="species" species="eucalyptorum">
|
||
<emphasis id="D1CEBE6D1006D1769E10F8832403FE28" box="[322,584,1812,1838]" italics="true" pageId="3" pageNumber="43">Coniella eucalyptorum</emphasis>
|
||
</taxonomicName>
|
||
was previously recorded in
|
||
<collectingCountry id="9BAD22EF1006D1769CCFF883224CFE28" box="[925,1031,1812,1838]" name="Australia" pageId="3" pageNumber="43">Australia</collectingCountry>
|
||
,
|
||
<collectingCountry id="9BAD22EF1006D1769B44F8832216FE28" box="[1046,1117,1812,1838]" name="Brazil" pageId="3" pageNumber="43">Brazil</collectingCountry>
|
||
,
|
||
<collectingCountry id="9BAD22EF1006D1769B3CF88322E6FE28" box="[1134,1197,1812,1838]" name="Chile" pageId="3" pageNumber="43">Chile</collectingCountry>
|
||
,
|
||
<collectingCountry id="9BAD22EF1006D1769BECF8832365FE28" box="[1214,1326,1812,1838]" name="Indonesia" pageId="3" pageNumber="43">Indonesia</collectingCountry>
|
||
,
|
||
<collectingCountry id="9BAD22EF1006D1769A6CF88323ECFE28" box="[1342,1447,1812,1838]" name="Malaysia" pageId="3" pageNumber="43">Malaysia</collectingCountry>
|
||
,
|
||
<collectingCountry id="9BAD22EF1006D1769FDAF8AF26ABFE54" box="[136,224,1848,1874]" name="Mexico" pageId="3" pageNumber="43">Mexico</collectingCountry>
|
||
,
|
||
<collectingCountry id="9BAD22EF1006D1769FBFF8AF2718FE54" box="[237,339,1848,1874]" name="Thailand" pageId="3" pageNumber="43">Thailand</collectingCountry>
|
||
,
|
||
<collectingCountry id="9BAD22EF1006D1769E0DF8AF279EFE54" box="[351,469,1848,1874]" name="Venezuela" pageId="3" pageNumber="43">Venezuela</collectingCountry>
|
||
, and
|
||
<collectingCountry id="9BAD22EF1006D1769D40F8AF243AFE54" box="[530,625,1848,1874]" name="Vietnam" pageId="3" pageNumber="43">Vietnam</collectingCountry>
|
||
(
|
||
<bibRefCitation id="872B1F8E1006D1769DD3F8AF250CFE54" author="Hyde, K. D. & de Silva, N. I. & Jeewon, R. & Bhat, D. J. & Phookamsak, R. & Doilom, M. & Boonmee, S. & Jayawardena, R. S. & Maharachchikumbura, S. S. N. & Senanayake, I. C. & Manawasinghe, I. S. & Liu, N. & Abeywickrama, P. D. & Chaiwan, N. & Karunarathna, A. & Pem, D. & Lin, C. G. & Sysouphanthong, P. & Luo, Z. L. & Wei, D. P. & Wanasinghe, D. N. & Norphanphoun, C. & Tennakoon, D. S. & Samarakoon, M. C. & Jayasiri, S. C. & Jiang, H. B. & Zeng, X. Y. & Li, J. F. & Wijesinghe, S. N. & Devadatha, B. & Goonasekara, I. D. & Brahmanage, R. S. & Yang, E. F. & Aluthmuhandiram, J. V. S. & Dayarathne, M. C. & Marasinghe, D. S. & Li, W. J. & Dissanayake, L. S. & Dong, W. & Huanraluek, N. & Lumyong, S. & Liu, J. K. & Karunarathna, S. C. & Jones, E. B. G. & Al-Sadi, A. M. & Xu, J. C. & Harishchandra, D. & Sarma, V. V. & Bulgakov, T. S." box="[641,839,1848,1875]" pageId="3" pageNumber="43" pagination="22 - 294" refId="ref5858" refString="Hyde, K. D., de Silva, N. I., Jeewon, R., Bhat, D. J., Phookamsak, R., Doilom, M., Boonmee, S., Jayawardena, R. S., Maharachchikumbura, S. S. N., Senanayake, I. C., Manawasinghe, I. S., Liu, N., Abeywickrama, P. D., Chaiwan, N., Karunarathna, A., Pem, D., Lin, C. G., Sysouphanthong, P., Luo, Z. L., Wei, D. P., Wanasinghe, D. N., Norphanphoun, C., Tennakoon, D. S., Samarakoon, M. C., Jayasiri, S. C., Jiang, H. B., Zeng, X. Y., Li, J. F., Wijesinghe, S. N., Devadatha, B., Goonasekara, I. D., Brahmanage, R. S., Yang, E. F., Aluthmuhandiram, J. V. S., Dayarathne, M. C., Marasinghe, D. S., Li, W. J., Dissanayake, L. S., Dong, W., Huanraluek, N., Lumyong, S., Liu, J. K., Karunarathna, S. C., Jones, E. B. G., Al-Sadi, A. M., Xu, J. C., Harishchandra, D., Sarma, V. V. & Bulgakov, T. S. (2020) AJOM new records and collections of fungi: 1 - 100. Asian Journal of Mycology 3: 22 - 294. https: // doi. org / 10.5943 / ajom / 3 / 1 / 3" type="journal article" year="2020">
|
||
Hyde
|
||
<emphasis id="D1CEBE6D1006D1769D9AF8AE24B1FE54" box="[712,762,1848,1874]" italics="true" pageId="3" pageNumber="43">et al</emphasis>
|
||
. 2020
|
||
</bibRefCitation>
|
||
). Therefore, we identified our fungal collection as the first geographical record from
|
||
<collectingCountry id="9BAD22EF1006D1769EB2F8CB241AFE70" box="[480,593,1884,1910]" name="Sri Lanka" pageId="3" pageNumber="43">Sri Lanka</collectingCountry>
|
||
.
|
||
</paragraph>
|
||
<caption id="B7C532F71001D1719FDAF8E827A2F120" ID-DOI="http://doi.org/10.5281/zenodo.10361449" ID-Zenodo-Dep="10361449" httpUri="https://zenodo.org/record/10361449/files/figure.png" pageId="4" pageNumber="44" startId="4.[136,229,1919,1941]" targetBox="[298,1288,187,1892]" targetPageId="4" targetType="figure">
|
||
<paragraph id="E305627F1001D1719FDAF8E827A2F120" blockId="4.[136,1452,1919,2086]" pageId="4" pageNumber="44">
|
||
<emphasis id="D1CEBE6D1001D1719FDAF8E826B4FE93" bold="true" box="[136,255,1919,1941]" pageId="4" pageNumber="44">FIGURE 1.</emphasis>
|
||
Phylogram generated for
|
||
<taxonomicName id="24BA19FC1001D1719EAFF81724D7FE90" box="[509,668,1920,1942]" pageId="4" pageNumber="44">
|
||
<emphasis id="D1CEBE6D1001D1719EAFF817241BFE93" box="[509,592,1920,1941]" italics="true" pageId="4" pageNumber="44">Coniella</emphasis>
|
||
species
|
||
</taxonomicName>
|
||
from ML and BI analyses based on a combined dataset of ITS, LSU, and
|
||
<emphasis id="D1CEBE6D1001D1719A21F81723E0FE93" box="[1395,1451,1920,1941]" italics="true" pageId="4" pageNumber="44">tef1-α</emphasis>
|
||
sequences. The tree is rooted with
|
||
<taxonomicName id="24BA19FC1001D1719EB1F83324D9FEBF" box="[483,658,1956,1977]" class="Sordariomycetes" family="Melanconidaceae" genus="Melanconiella" kingdom="Fungi" order="Diaporthales" pageId="4" pageNumber="44" phylum="Ascomycota" rank="species" species="undetermined">
|
||
<emphasis id="D1CEBE6D1001D1719EB1F8332425FEBF" box="[483,622,1956,1977]" italics="true" pageId="4" pageNumber="44">Melanconiella</emphasis>
|
||
sp.
|
||
</taxonomicName>
|
||
(CBS110385). Bootstrap support values for ML ≥ 65 % and Bayesian posterior probabilities (PP) ≥ 0.95 are shown at the nodes. The strain from the current study is in red. All ex-type strains are in bold black. Some branches were shortened to fit them to the pages, and these are indicated by two diagonal lines with the number of times a branch was shortened indicated next to the lines.
|
||
</paragraph>
|
||
</caption>
|
||
<caption id="B7C532F71000D1709FDAFAC1273CFC6A" box="[136,375,1366,1388]" isContinuationCaption="true" pageId="5" pageNumber="45" startId="5.[136,229,1366,1388]" targetBox="[194,1394,190,1342]" targetPageId="5" targetType="figure">
|
||
<paragraph id="E305627F1000D1709FDAFAC1273CFC6A" blockId="5.[136,375,1366,1388]" box="[136,375,1366,1388]" pageId="5" pageNumber="45">
|
||
<emphasis id="D1CEBE6D1000D1709FDAFAC126B5FC6D" bold="true" box="[136,254,1366,1388]" pageId="5" pageNumber="45">FIGURE 1.</emphasis>
|
||
(Continued)
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="E305627F1000D1709FDAFA0C2722FCB3" blockId="5.[136,361,1435,1461]" box="[136,361,1435,1461]" pageId="5" pageNumber="45">
|
||
<emphasis id="D1CEBE6D1000D1709FDAFA0C2722FCB3" box="[136,361,1435,1461]" italics="true" pageId="5" pageNumber="45">Pathogenicity assay</emphasis>
|
||
</paragraph>
|
||
<paragraph id="E305627F1000D1709FDAFA74221FFE63" blockId="5.[136,1452,1507,1893]" pageId="5" pageNumber="45">
|
||
Results of the pathogenicity test showed that the RB-LP31 strain (identified as
|
||
<taxonomicName id="24BA19FC1000D1709B06FA742350FCFB" box="[1108,1307,1507,1533]" class="Sordariomycetes" family="Schizoparmaceae" genus="Coniella" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="45" phylum="Ascomycota" rank="species" species="eucalyptorum">
|
||
<emphasis id="D1CEBE6D1000D1709B06FA742350FCFB" box="[1108,1307,1507,1533]" italics="true" pageId="5" pageNumber="45">C. eucalyptorum</emphasis>
|
||
</taxonomicName>
|
||
) can cause disease on only wounded host leaves. The initial symptoms were seen on wounded leaves after two to three days of inoculations with mycelial plugs (
|
||
<figureCitation id="7B817EFA1000D1709DDAF9BC2499FF43" box="[648,722,1579,1605]" captionStart="FIGURE 3" captionStartId="7.[136,229,1921,1943]" captionTargetBox="[340,1247,189,1891]" captionTargetId="figure-24@7.[335,1253,187,1894]" captionTargetPageId="7" captionText="FIGURE 3. Pathogenicity test results for C. eucalyptorum on E. camaldulensis leaves. a1–a4 wounded leaves inoculated with C. eucalyptorum spore suspensions. b1–b2 non-wounded leaves inoculated with C. eucalyptorum spore suspensions. c1–c2 non-wounded leaves inoculated with sterile water (control). d1–d2 wounded leaves inoculated with sterile water (control). e1–e4 wounded leaves inoculated with C. eucalyptorum mycelial plugs. f1–f2 non-wounded leaves inoculated with C. eucalyptorum mycelial plugs. g1–g2 wounded leaves inoculated with sterile PDA plugs (control). h1–h2 non-wounded leaves inoculated with sterile PDA plugs (control)." figureDoi="http://doi.org/10.5281/zenodo.10361453" httpUri="https://zenodo.org/record/10361453/files/figure.png" pageId="5" pageNumber="45">Fig. 3</figureCitation>
|
||
, e
|
||
<figureCitation id="7B817EFA1000D1709DA3F9BC2551FF43" box="[753,794,1579,1605]" captionStart="FIGURE 1" captionStartId="4.[136,229,1919,1941]" captionTargetBox="[298,1288,187,1892]" captionTargetId="figure-18@4.[298,1289,187,1892]" captionTargetPageId="4" captionText="FIGURE 1. Phylogram generated for Coniella species from ML and BI analyses based on a combined dataset of ITS, LSU, and tef1-α sequences. The tree is rooted with Melanconiella sp. (CBS110385). Bootstrap support values for ML ≥ 65 % and Bayesian posterior probabilities (PP) ≥ 0.95 are shown at the nodes. The strain from the current study is in red. All ex-type strains are in bold black. Some branches were shortened to fit them to the pages, and these are indicated by two diagonal lines with the number of times a branch was shortened indicated next to the lines." figureDoi="http://doi.org/10.5281/zenodo.10361449" httpUri="https://zenodo.org/record/10361449/files/figure.png" pageId="5" pageNumber="45">
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1
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||
<emphasis id="D1CEBE6D1000D1709DAFF9BC2540FF43" bold="true" box="[765,779,1579,1605]" pageId="5" pageNumber="45">–</emphasis>
|
||
e
|
||
</figureCitation>
|
||
4). Small light-brown to brown lesions emerged at the inoculation site of the leaves. Subsequently, these lesions rapidly enlarged (diameter
|
||
<quantity id="2442CF9A1000D1709B3DF9D82296FF6F" box="[1135,1245,1615,1642]" metricMagnitude="-2" metricUnit="m" metricValue="3.75" metricValueMax="5.5" metricValueMin="2.0" pageId="5" pageNumber="45" unit="cm" value="3.75" valueMax="5.5" valueMin="2.0">
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2
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||
<emphasis id="D1CEBE6D1000D1709B2CF9D822C7FF6F" bold="true" box="[1150,1164,1615,1641]" pageId="5" pageNumber="45">–</emphasis>
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5.5 cm
|
||
</quantity>
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||
) and transformed into brown to dark brown lesions after five days of inoculation. Symptoms continuously spread, and sparse white mycelia appeared on the lesions three days post-inoculation and subsequently spread throughout the host leaf. Initial symptoms were seen on wounded leaves treated with spore suspensions of
|
||
<taxonomicName id="24BA19FC1000D1709BB6F92C23E0FFD3" box="[1252,1451,1723,1749]" class="Sordariomycetes" family="Schizoparmaceae" genus="Coniella" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="45" phylum="Ascomycota" rank="species" species="eucalyptorum">
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||
<emphasis id="D1CEBE6D1000D1709BB6F92C23E0FFD3" box="[1252,1451,1723,1749]" italics="true" pageId="5" pageNumber="45">C. eucalyptorum</emphasis>
|
||
</taxonomicName>
|
||
after 10–12 days of inoculation (
|
||
<figureCitation id="7B817EFA1000D1709D4DF9482420FFFF" box="[543,619,1759,1785]" captionStart="FIGURE 3" captionStartId="7.[136,229,1921,1943]" captionTargetBox="[340,1247,189,1891]" captionTargetId="figure-24@7.[335,1253,187,1894]" captionTargetPageId="7" captionText="FIGURE 3. Pathogenicity test results for C. eucalyptorum on E. camaldulensis leaves. a1–a4 wounded leaves inoculated with C. eucalyptorum spore suspensions. b1–b2 non-wounded leaves inoculated with C. eucalyptorum spore suspensions. c1–c2 non-wounded leaves inoculated with sterile water (control). d1–d2 wounded leaves inoculated with sterile water (control). e1–e4 wounded leaves inoculated with C. eucalyptorum mycelial plugs. f1–f2 non-wounded leaves inoculated with C. eucalyptorum mycelial plugs. g1–g2 wounded leaves inoculated with sterile PDA plugs (control). h1–h2 non-wounded leaves inoculated with sterile PDA plugs (control)." figureDoi="http://doi.org/10.5281/zenodo.10361453" httpUri="https://zenodo.org/record/10361453/files/figure.png" pageId="5" pageNumber="45">Fig. 3</figureCitation>
|
||
, a
|
||
<figureCitation id="7B817EFA1000D1709DD9F94824FFFFFF" box="[651,692,1759,1785]" captionStart="FIGURE 1" captionStartId="4.[136,229,1919,1941]" captionTargetBox="[298,1288,187,1892]" captionTargetId="figure-18@4.[298,1289,187,1892]" captionTargetPageId="4" captionText="FIGURE 1. Phylogram generated for Coniella species from ML and BI analyses based on a combined dataset of ITS, LSU, and tef1-α sequences. The tree is rooted with Melanconiella sp. (CBS110385). Bootstrap support values for ML ≥ 65 % and Bayesian posterior probabilities (PP) ≥ 0.95 are shown at the nodes. The strain from the current study is in red. All ex-type strains are in bold black. Some branches were shortened to fit them to the pages, and these are indicated by two diagonal lines with the number of times a branch was shortened indicated next to the lines." figureDoi="http://doi.org/10.5281/zenodo.10361449" httpUri="https://zenodo.org/record/10361449/files/figure.png" pageId="5" pageNumber="45">
|
||
1
|
||
<emphasis id="D1CEBE6D1000D1709DC5F94824EEFFFF" bold="true" box="[663,677,1759,1785]" pageId="5" pageNumber="45">–</emphasis>
|
||
a
|
||
</figureCitation>
|
||
4), and
|
||
<quantity id="2442CF9A1000D1709C41F9482525FFFF" box="[787,878,1759,1785]" metricMagnitude="-2" metricUnit="m" metricValue="2.0" metricValueMax="3.0" metricValueMin="1.0" pageId="5" pageNumber="45" unit="cm" value="2.0" valueMax="3.0" valueMin="1.0">1–3 cm</quantity>
|
||
lesions appeared after 16 days of inoculation. Non-wounded leaves inoculated with both spore suspensions and mycelial plugs showed no symptoms even after 16 days of inoculation (
|
||
<figureCitation id="7B817EFA1000D1709E20F8B027FCFE47" box="[370,439,1831,1857]" captionStart="FIGURE 3" captionStartId="7.[136,229,1921,1943]" captionTargetBox="[340,1247,189,1891]" captionTargetId="figure-24@7.[335,1253,187,1894]" captionTargetPageId="7" captionText="FIGURE 3. Pathogenicity test results for C. eucalyptorum on E. camaldulensis leaves. a1–a4 wounded leaves inoculated with C. eucalyptorum spore suspensions. b1–b2 non-wounded leaves inoculated with C. eucalyptorum spore suspensions. c1–c2 non-wounded leaves inoculated with sterile water (control). d1–d2 wounded leaves inoculated with sterile water (control). e1–e4 wounded leaves inoculated with C. eucalyptorum mycelial plugs. f1–f2 non-wounded leaves inoculated with C. eucalyptorum mycelial plugs. g1–g2 wounded leaves inoculated with sterile PDA plugs (control). h1–h2 non-wounded leaves inoculated with sterile PDA plugs (control)." figureDoi="http://doi.org/10.5281/zenodo.10361453" httpUri="https://zenodo.org/record/10361453/files/figure.png" pageId="5" pageNumber="45">Fig. 3</figureCitation>
|
||
, b and f), similar to the negative control treatments (
|
||
<figureCitation id="7B817EFA1000D1709B45F8B02217FE47" box="[1047,1116,1831,1857]" captionStart="FIGURE 3" captionStartId="7.[136,229,1921,1943]" captionTargetBox="[340,1247,189,1891]" captionTargetId="figure-24@7.[335,1253,187,1894]" captionTargetPageId="7" captionText="FIGURE 3. Pathogenicity test results for C. eucalyptorum on E. camaldulensis leaves. a1–a4 wounded leaves inoculated with C. eucalyptorum spore suspensions. b1–b2 non-wounded leaves inoculated with C. eucalyptorum spore suspensions. c1–c2 non-wounded leaves inoculated with sterile water (control). d1–d2 wounded leaves inoculated with sterile water (control). e1–e4 wounded leaves inoculated with C. eucalyptorum mycelial plugs. f1–f2 non-wounded leaves inoculated with C. eucalyptorum mycelial plugs. g1–g2 wounded leaves inoculated with sterile PDA plugs (control). h1–h2 non-wounded leaves inoculated with sterile PDA plugs (control)." figureDoi="http://doi.org/10.5281/zenodo.10361453" httpUri="https://zenodo.org/record/10361453/files/figure.png" pageId="5" pageNumber="45">Fig. 3</figureCitation>
|
||
, c, d, g, and h). Based on the morphological characteristics, we identified the re-isolated fungus as
|
||
<taxonomicName id="24BA19FC1000D1709CC0F8DC2206FE63" box="[914,1101,1867,1893]" class="Sordariomycetes" family="Schizoparmaceae" genus="Coniella" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="45" phylum="Ascomycota" rank="species" species="eucalyptorum">
|
||
<emphasis id="D1CEBE6D1000D1709CC0F8DC2206FE63" box="[914,1101,1867,1893]" italics="true" pageId="5" pageNumber="45">C. eucalyptorum</emphasis>
|
||
</taxonomicName>
|
||
.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |