350 lines
40 KiB
XML
350 lines
40 KiB
XML
<document id="18547E3AF08D731E269CA52B6EE678A3" ID-DOI="10.4467/16890027AP.21.002.14062" ID-ISSN="1689-0027" IM.bibliography_approvedBy="carolina" IM.illustrations_approvedBy="carolina" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="carolina" IM.tables_approvedBy="carolina" IM.taxonomicNames_approvedBy="carolina" IM.treatments_approvedBy="carolina" checkinTime="1713467996671" checkinUser="felipe" docAuthor="Solarczyk, Piotr" docDate="2021" docId="226F87B07B140E06BD42FAE55FEFF8E0" docLanguage="en" docName="ActaProtozool.60.1.13-20.pdf.imf" docOrigin="Acta Protozoologica 60" docSource="http://dx.doi.org/10.4467/16890027ap.21.002.14062" docStyle="DocumentStyle:64ABA4BEA387EE5C24A0DDEB32CC92E7.3:ActaProtozool.2014-.journal_article" docStyleId="64ABA4BEA387EE5C24A0DDEB32CC92E7" docStyleName="ActaProtozool.2014-.journal_article" docStyleVersion="3" docTitle="Cyclospora Schneider 1881" docType="treatment" docVersion="3" lastPageNumber="17" masterDocId="DE56FFC87B170E02BE6BFF9E5C7CFFB0" masterDocTitle="Host Range of Cyclospora Species: Zoonotic Implication" masterLastPageNumber="20" masterPageNumber="13" pageNumber="16" updateTime="1715176107845" updateUser="carolina" zenodo-license-document="CC-BY-4.0">
|
|
<mods:mods id="AA8F48848F8405BF41936A0D34A6315E" xmlns:mods="http://www.loc.gov/mods/v3">
|
|
<mods:titleInfo id="CCBC59321C056BE4511EE37D2064F248">
|
|
<mods:title id="A072F70E2348B1C5D6DB5F93A4BC1B3D">Host Range of Cyclospora Species: Zoonotic Implication</mods:title>
|
|
</mods:titleInfo>
|
|
<mods:name id="318F13862BC076D6D5AD25A9F0F2D6AC" type="personal">
|
|
<mods:role id="36612EAE68CE3CF3A467F8A37370B4BC">
|
|
<mods:roleTerm id="7EC0794BEFDE6F18A3B51693FFF1C6B9">Author</mods:roleTerm>
|
|
</mods:role>
|
|
<mods:namePart id="5A06A8B36B4AD4B982BF54BBB26FE01A">Solarczyk, Piotr</mods:namePart>
|
|
</mods:name>
|
|
<mods:typeOfResource id="9CE9D89DAAB7E92FF339231CFF03488F">text</mods:typeOfResource>
|
|
<mods:relatedItem id="39A2872DFBCF58C14AE6B7015E6558EB" type="host">
|
|
<mods:titleInfo id="8DFA2FC9E984B74995BD583DADB432E4">
|
|
<mods:title id="A405499BDB4C5E81092FD108A7A501EA">Acta Protozoologica</mods:title>
|
|
</mods:titleInfo>
|
|
<mods:part id="A3C276B710F344619E4A4CB1D45BF993">
|
|
<mods:date id="9CDD8158A2FE8435D5AB8598EC7A9474">2021</mods:date>
|
|
<mods:detail id="A1445C9F82B747474D85FF1DF32C6C41" type="pubDate">
|
|
<mods:number id="E25ABECFF05AB43EDF292C7A3A3B62A6">2021-12-31</mods:number>
|
|
</mods:detail>
|
|
<mods:detail id="23E712F881098E986ED7D1BC1A7BB830" type="volume">
|
|
<mods:number id="61237070996839E175C8A0466A081D22">60</mods:number>
|
|
</mods:detail>
|
|
<mods:extent id="F172FEB4351DE68793AC0C95A13ABE33" unit="page">
|
|
<mods:start id="D3A4AC6CEE8FC86C221F9F382E3DCD6F">13</mods:start>
|
|
<mods:end id="07E668BF7D8E599AD9C28F2CAEA80DC7">20</mods:end>
|
|
</mods:extent>
|
|
</mods:part>
|
|
</mods:relatedItem>
|
|
<mods:location id="09A91E324EDDD5BF15FB4808A59C7609">
|
|
<mods:url id="8C2586B869B54D4529939ABDCC2E164C">http://dx.doi.org/10.4467/16890027ap.21.002.14062</mods:url>
|
|
</mods:location>
|
|
<mods:classification id="955390B293BBE93C83ADBBC2492A090B">journal article</mods:classification>
|
|
<mods:identifier id="F46D74FF87023B6519CDDDFAF47B5F6B" type="DOI">10.4467/16890027AP.21.002.14062</mods:identifier>
|
|
<mods:identifier id="DA5ECE651919C013A8EF2DAB49BDA683" type="ISSN">1689-0027</mods:identifier>
|
|
</mods:mods>
|
|
<treatment id="226F87B07B140E06BD42FAE55FEFF8E0" LSID="urn:lsid:plazi:treatment:226F87B07B140E06BD42FAE55FEFF8E0" httpUri="http://treatment.plazi.org/id/226F87B07B140E06BD42FAE55FEFF8E0" lastPageId="4" lastPageNumber="17" pageId="3" pageNumber="16">
|
|
<subSubSection id="E2DC652D7B140E01BD42FAE55FF7FA08" pageId="3" pageNumber="16" type="nomenclature">
|
|
<paragraph id="AA7936A67B140E01BD42FAE55FF7FA08" blockId="3.[809,1455,1402,1464]" pageId="3" pageNumber="16">
|
|
<heading id="F13181CA7B140E01BD42FAE55FF7FA08" centered="true" fontSize="11" level="2" pageId="3" pageNumber="16" reason="5">
|
|
<emphasis id="98B2EAB47B140E01BD42FAE55FF7FA08" bold="true" pageId="3" pageNumber="16">
|
|
Drawbacks of
|
|
<taxonomicName id="6DC64D257B140E01BD81FAE4580DFA25" ID-CoL="3Y48" authority="Schneider, 1881" authorityName="Schneider" authorityYear="1881" box="[1002,1137,1402,1429]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="3" pageNumber="16" phylum="Miozoa" rank="genus">
|
|
<emphasis id="98B2EAB47B140E01BD81FAE4580DFA25" bold="true" box="[1002,1137,1402,1429]" italics="true" pageId="3" pageNumber="16">Cyclospora</emphasis>
|
|
</taxonomicName>
|
|
molecular diagnostic in animals
|
|
</emphasis>
|
|
</heading>
|
|
</paragraph>
|
|
</subSubSection>
|
|
<subSubSection id="E2DC652D7B140E06BD24FA4C5FEFF8E0" lastPageId="4" lastPageNumber="17" pageId="3" pageNumber="16" type="description">
|
|
<paragraph id="AA7936A67B140E06BD24FA4C5D6EFAB5" blockId="3.[809,1456,1489,1945]" lastBlockId="4.[132,779,223,1286]" lastPageId="4" lastPageNumber="17" pageId="3" pageNumber="16">
|
|
As a result of the limitation of the microscopic assay, molecular-based methods have been developed for the detection of
|
|
<taxonomicName id="6DC64D257B140E01BD82F987580CF983" box="[1001,1136,1561,1587]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="3" pageNumber="16" phylum="Miozoa" rank="genus">
|
|
<emphasis id="98B2EAB47B140E01BD82F987580CF983" box="[1001,1136,1561,1587]" italics="true" pageId="3" pageNumber="16">Cyclospora</emphasis>
|
|
</taxonomicName>
|
|
in various
|
|
<typeStatus id="757D88047B140E01BA9EF9875954F983" box="[1269,1320,1561,1587]" pageId="3" pageNumber="16">type</typeStatus>
|
|
of samples to assess infection risk (
|
|
<bibRefCitation id="CE574B577B140E01BA2AF9A35940F9E7" author="Chacin-Bonilla L." box="[1089,1340,1597,1623]" pageId="3" pageNumber="16" pagination="215 - 216" refId="ref4224" refString="Chacin-Bonilla L. (2008) Transmission of Cyclospora cayetanensis infection: A review focusing on soil-borne cyclosporiasis. Trans. R. Soc. Trop. Med. Hyg. 102: 215 - 216" type="journal article" year="2008">Chacín-Bonilla 2008</bibRefCitation>
|
|
;
|
|
<bibRefCitation id="CE574B577B140E01BB23F9A35FD4F9CB" author="Kitajima M. & Haramoto E. & Iker B. C. & Gerba C. P." pageId="3" pageNumber="16" pagination="129 - 136" refId="ref5182" refString="Kitajima M., Haramoto E., Iker B. C., Gerba C. P. (2014) Occur- rence of Cryptosporidium, Giardia, and Cyclospora in influent and effluent water at wastewater treatment plants in Arizona. Sci. Total Environ. 484: 129 - 136" type="journal article" year="2014">
|
|
Kitajima
|
|
<emphasis id="98B2EAB47B140E01BD42F9FF5F1FF9CA" box="[809,867,1632,1658]" italics="true" pageId="3" pageNumber="16">et al.</emphasis>
|
|
2014
|
|
</bibRefCitation>
|
|
;
|
|
<bibRefCitation id="CE574B577B140E01BDDFF9FE58E1F9CB" author="Lalonde L. F. & Gajadhar A. A." box="[948,1181,1632,1659]" pageId="3" pageNumber="16" pagination="8 - 14" refId="ref5374" refString="Lalonde L. F. and Gajadhar A. A. (2016) Detection of Cyclospora cayetanensis, Cryptosporidium spp., and Toxoplasma gondii on imported leafy green vegetables in Canadian survey. Food Waterborne Parasitol. 2: 8 - 14" type="journal article" year="2016">
|
|
Lalonde
|
|
<emphasis id="98B2EAB47B140E01BA77F9FF582AF9CA" box="[1052,1110,1632,1658]" italics="true" pageId="3" pageNumber="16">et al.</emphasis>
|
|
2016
|
|
</bibRefCitation>
|
|
). To establish a reliable zoonotic outcome, microscopic analysis must be supported by molecular results. Because the pathogen is usually present in very low numbers in fecal samples, the detection is a very challenging task. In humans, molecular assays for
|
|
<taxonomicName id="6DC64D257B140E01BA62F88D58ECF89D" box="[1033,1168,1811,1837]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="3" pageNumber="16" phylum="Miozoa" rank="genus">
|
|
<emphasis id="98B2EAB47B140E01BA62F88D58ECF89D" box="[1033,1168,1811,1837]" italics="true" pageId="3" pageNumber="16">Cyclospora</emphasis>
|
|
</taxonomicName>
|
|
detection are primarily dependent on the quality and purity of the genetic material, so
|
|
<emphasis id="98B2EAB47B140E01BDF3F8C25F8FF8C5" box="[920,1011,1884,1909]" italics="true" pageId="3" pageNumber="16">a priori</emphasis>
|
|
choice of DNA extraction method to isolate parasite genetic material from animals is a crucial step as well (
|
|
<bibRefCitation id="CE574B577B130E06BF27FF415E4EFF4A" author="da Silva A. J. & Bornay-Llinares F. J. & Moura I. N. & Slemenda S. B. & Tuttle J. L. & Pieniazek N. J." box="[332,562,223,250]" pageId="4" pageNumber="17" pagination="57 - 64" refId="ref4469" refString="da Silva A. J., Bornay-Llinares F. J., Moura I. N., Slemenda S. B., Tuttle J. L., Pieniazek N. J. (1999) Fast and reliable extraction of protozoan parasite DNA from fecal specimens. Mol. Diagn. 4: 57 - 64" type="journal article" year="1999">
|
|
da Silva
|
|
<emphasis id="98B2EAB47B130E06BFD8FF7E5D91FF49" box="[435,493,223,249]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
1999
|
|
</bibRefCitation>
|
|
;
|
|
<bibRefCitation id="CE574B577B130E06BC55FF7E5CBEFEAD" author="Qvarnstrom Y. & Wei-Pridgeon Y. & Li W. & Nascimento F. S. & Bishop H. S. & Herwaldt B. L. & Moss D. M. & Nayak V. & Srinivasamoorthy G. & Sheth M." pageId="4" pageNumber="17" pagination="6" refId="ref6894" refString="Qvarnstrom Y., Wei-Pridgeon Y., Li W., Nascimento F. S., Bishop H. S., Herwaldt B. L., Moss D. M., Nayak V., Srinivasamoorthy G., Sheth M. et al. (2015) Draft genome sequences from Cyclospora cayetanensis oocysts purified from a human stool sample. Genome Announc. 3 (6): e 01324 - 15" type="journal article" year="2015">
|
|
Qvarnstrom
|
|
<emphasis id="98B2EAB47B130E06BCBAFF7E5F77FF49" box="[721,779,223,249]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2015
|
|
</bibRefCitation>
|
|
;
|
|
<bibRefCitation id="CE574B577B130E06BEBAFE9C5DD1FEAC" author="Paulos S. & Mateo M. & de Lucio A. & Hernandez-de & Mingo M. & Begona Bailo B. & Saugar J. M. & Cardona G. A. & Fuentes I. & Mateo M. & Carmena D." box="[209,429,258,284]" pageId="4" pageNumber="17" pagination="520 - 528" refId="ref6348" refString="Paulos S., Mateo M., de Lucio A., Hernandez-de Mingo M., Begona Bailo B., Saugar J. M., Cardona G. A., Fuentes I., Mateo M., Carmena D. (2016) Evaluation of five commercial methods for the extraction and purification of DNA from human feacal samples for downstream molecular detection of the enteric protozoan parasites Cryptosporidium spp., Giardia duodenalis and Enatamoeba spp. J. Microbiol. Methods. 21: 520 - 528" type="journal article" year="2016">
|
|
Paulos
|
|
<emphasis id="98B2EAB47B130E06BF43FE9D5D19FEAC" box="[296,357,258,284]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2016
|
|
</bibRefCitation>
|
|
; Qvarnstrom
|
|
<emphasis id="98B2EAB47B130E06BC3AFE9D5EF2FEAC" box="[593,654,258,284]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2018). To date, the data of differences in usability of commercially available DNA extraction kits in animals fecal samples is restricted. To overcome current molecular genotyping problems, three genetic loci such a region within the small subunit ribosomal RNA gene (
|
|
<collectionCode id="CCD7AE637B130E06BC1BFE315EDBFE79" box="[624,679,431,457]" country="0" httpUri="http://grbio.org/cool/fye2-vfes" name="Saratov State University" pageId="4" pageNumber="17">SSU</collectionCode>
|
|
rRNA), the 70 kilodalton heat shock protein (
|
|
<collectionCode id="CCD7AE637B130E06BC5EFE4F5E11FE5B" box="[565,621,465,491]" pageId="4" pageNumber="17">HSP</collectionCode>
|
|
70) gene, and the ribosomal internal transcribed spacer (
|
|
<collectionCode id="CCD7AE637B130E06BCF8FE6A5EBDFDBE" box="[659,705,500,526]" pageId="4" pageNumber="17">ITS</collectionCode>
|
|
) (
|
|
<bibRefCitation id="CE574B577B130E06BC8AFE6A5D1BFD80" author="Sulaiman I. M. & Torres P. & Simpson S. & Kerdahi K. & Ortega Y." pageId="4" pageNumber="17" pagination="379 - 382" refId="ref7344" refString="Sulaiman I. M., Torres P., Simpson S., Kerdahi K., Ortega Y. (2013) Sequence characterization of heat shock protein gene of Cyclospora cayetanensis isolates from Nepal, Mexico, and Peru. J. Parasitol. 99: 379 - 382" type="journal article" year="2013">
|
|
Sulaiman
|
|
<emphasis id="98B2EAB47B130E06BEB4FD895D62FD80" box="[223,286,534,560]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2013
|
|
</bibRefCitation>
|
|
;
|
|
<bibRefCitation id="CE574B577B130E06BF1CFD885E27FD80" author="Olivier C. & van de Pas S. & Lepp P. W. & Yoder K. & Relman D. A." box="[375,603,534,560]" pageId="4" pageNumber="17" pagination="1475 - 1487" refId="ref6214" refString="Olivier C., van de Pas S., Lepp P. W., Yoder K., Relman D. A. (2001) Sequence variability in the first internal transcribed spacer re- gion within and among Cyclospora species is consistent with polyparasitism. Int. J. Parasitol. 31: 1475 - 1487" type="journal article" year="2001">
|
|
Olivier
|
|
<emphasis id="98B2EAB47B130E06BFBDFD895E68FD80" box="[470,532,534,560]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2001
|
|
</bibRefCitation>
|
|
) were primarily developed to improved detection
|
|
<taxonomicName id="6DC64D257B130E06BC26FDA75CBEFDC5" authority="DNA" authorityName="DNA" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="species" species="cayetanensis">
|
|
<emphasis id="98B2EAB47B130E06BC26FDA75F77FDE2" box="[589,779,569,595]" italics="true" pageId="4" pageNumber="17">C. cayetanensis</emphasis>
|
|
DNA
|
|
</taxonomicName>
|
|
in human fecal samples. According to the literature, SSU-rDNA and
|
|
<collectionCode id="CCD7AE637B130E06BFF3FDE05DB8FD28" box="[408,452,638,664]" pageId="4" pageNumber="17">ITS</collectionCode>
|
|
gene fragments were used for
|
|
<taxonomicName id="6DC64D257B130E06BEC4FD3E5D4AFD0A" box="[175,310,672,698]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="genus">
|
|
<emphasis id="98B2EAB47B130E06BEC4FD3E5D4AFD0A" box="[175,310,672,698]" italics="true" pageId="4" pageNumber="17">Cyclospora</emphasis>
|
|
</taxonomicName>
|
|
typing in animals (
|
|
<bibRefCitation id="CE574B577B130E06BC75FD3E5F7AFD0A" author="Relman D. A. & Schmidt T. M. & Gajadhar A. & Sogin M. & Cross J. & Yoder K. & Sethabutr O. & Echeverria P." box="[542,774,672,698]" pageId="4" pageNumber="17" pagination="440 - 445" refId="ref7039" refString="Relman D. A., Schmidt T. M., Gajadhar A., Sogin M., Cross J., Yoder K., Sethabutr O., Echeverria P. (1996) Molecular phylogenetic analysis of Cyclospora, the human intestinal pathogen, suggests that it is closely related to Eimeria species. J. Infect. Dis. 173: 440 - 445" type="journal article" year="1996">
|
|
Relman
|
|
<emphasis id="98B2EAB47B130E06BCE8FD3F5EC3FD0A" box="[643,703,672,698]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
1996
|
|
</bibRefCitation>
|
|
; 30
|
|
<bibRefCitation id="CE574B577B130E06BEC6FD5D5D02FD6D" author="Zhao G. H. & Cong M. M. & Bian Q. Q. & Cheng W. Y. & Wang R. J. & Qi M. & Zhang L. X. & Lin Q. & Zhu X. Q." box="[173,382,707,733]" pageId="4" pageNumber="17" pagination="58216" refId="ref7606" refString="Zhao G. H., Cong M. M. Bian Q. Q., Cheng W. Y., Wang R. J., Qi M., Zhang L. X., Lin Q., Zhu X. Q. (2013) Molecular characterization of Cyclospora - like organisms from golden snub-nosed monkeys in Qinling Mountain in Shaanxi province, northwestern China. PLoS ONE 8: e 58216" type="journal article" year="2013">
|
|
Zhao
|
|
<emphasis id="98B2EAB47B130E06BE9FFD5A5D4FFD6D" box="[244,307,707,733]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2013
|
|
</bibRefCitation>
|
|
). However, some caution should be required as the
|
|
<taxonomicName id="6DC64D257B130E06BF0BFD785E66FD4F" box="[352,538,742,768]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="species" species="cayetanensis">
|
|
<emphasis id="98B2EAB47B130E06BF0BFD785E66FD4F" box="[352,538,742,768]" italics="true" pageId="4" pageNumber="17">C. cayetanensis</emphasis>
|
|
</taxonomicName>
|
|
populations may be heterogeneous. The pathogen is a sexually reproducing organism and any isolate may have genetically heterogeneous sequences. Through this process, sporozoites in a single sporocyst are thought to be genetically identical, while the sporocysts in a single oocyst can be genetically distinct (
|
|
<bibRefCitation id="CE574B577B130E06BF3CFC2A5E4BFC7E" author="Shirley M. W. & Harvey D. A." box="[343,567,948,974]" pageId="4" pageNumber="17" pagination="523 - 528" refId="ref7237" refString="Shirley M. W., Harvey D. A. (1996) Eimeria tenella: infection with a single sporocyst gives a clonal population. Parasitology. 112: 523 - 528" type="journal article" year="1996">
|
|
Shirley
|
|
<emphasis id="98B2EAB47B130E06BFDDFC2B5D8DFC7E" box="[438,497,948,974]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
1996
|
|
</bibRefCitation>
|
|
;
|
|
<bibRefCitation id="CE574B577B130E06BC2FFC2A5CB8FC41" author="Mzilahowa T. & McCall P. J. & Hastings I. M." pageId="4" pageNumber="17" pagination="613" refId="ref5973" refString="Mzilahowa T., McCall P. J., Hastings I. M. (2007) ' Sexual' population structure and genetics of the malaria agent P. falciparum. PLoS ONE 2: e 613" type="journal article" year="2007">
|
|
Mzilahowa
|
|
<emphasis id="98B2EAB47B130E06BCA4FC2B5F77FC7E" box="[719,779,948,974]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2007
|
|
</bibRefCitation>
|
|
). Therefore, one
|
|
<taxonomicName id="6DC64D257B130E06BFFEFC495E60FC41" box="[405,540,983,1009]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="genus">
|
|
<emphasis id="98B2EAB47B130E06BFFEFC495E60FC41" box="[405,540,983,1009]" italics="true" pageId="4" pageNumber="17">Cyclospora</emphasis>
|
|
</taxonomicName>
|
|
oocyst is heterozygous, possessing up to two alleles for any given marker and amplicons may vary in their sequences. New genotyping information for
|
|
<taxonomicName id="6DC64D257B130E06BFF4FBA15E27FBE8" box="[415,603,1087,1113]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="species" species="cayetanensis">
|
|
<emphasis id="98B2EAB47B130E06BFF4FBA15E27FBE8" box="[415,603,1087,1113]" italics="true" pageId="4" pageNumber="17">C. cayetanensis</emphasis>
|
|
</taxonomicName>
|
|
, derived from mitochondrial genome markers, should be helpful in animal source tracking studies. Next-Generation Sequencing (
|
|
<collectionCode id="CCD7AE637B130E06BF6AFB385D43FB70" box="[257,319,1190,1216]" pageId="4" pageNumber="17">NGS</collectionCode>
|
|
) is the best technique for such studies (
|
|
<bibRefCitation id="CE574B577B130E06BEE7FB575DD5FB53" author="Nascimento F. S. & Barta J. R. & Wale J. & Hofstetter J. N. & Casillas S. & Barratt J. & Talundzic E. & Arrowood M. J. & Qvarnstrom Y." box="[140,425,1225,1251]" pageId="4" pageNumber="17" pagination="1307 - 1312" refId="ref6077" refString="Nascimento F. S., Barta J. R., Wale J., Hofstetter J. N., Casillas S., Barratt J., Talundzic E., Arrowood M. J., Qvarnstrom Y. (2019) Mitochondrial junction region as genotyping marker for Cyclospora cayetanensis. Emerg. Infect. Dis. 25: 1307 - 1312" type="journal article" year="2019">
|
|
Nascimento
|
|
<emphasis id="98B2EAB47B130E06BF4FFB575D1DFB53" box="[292,353,1225,1251]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2019
|
|
</bibRefCitation>
|
|
;
|
|
<bibRefCitation id="CE574B577B130E06BFD3FB575EC0FB53" author="Houghton K. A. & Lomsadze A. & Park S. & Nascimento F. S. & Barrat J. & Arrowood M. J. & VanRoey E. & Talundzic E. & Borodovsky M. & Qwarnstrom Y." box="[440,700,1225,1251]" pageId="4" pageNumber="17" pagination="24" refId="ref5114" refString="Houghton K. A., Lomsadze A., Park S., Nascimento F. S., Barrat J., Arrowood M. J., VanRoey E., Talundzic E., Borodovsky M., Qwarnstrom Y. (2020) Development of a workflow for identifi- cation of nuclear genotyping markers for Cyclospora cayetanensis. Parasite. 27: 24" type="journal article" year="2020">
|
|
Houghton
|
|
<emphasis id="98B2EAB47B130E06BC5CFB575E08FB53" box="[567,628,1225,1251]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2020
|
|
</bibRefCitation>
|
|
,
|
|
<bibRefCitation id="CE574B577B130E06BCA2FB575D7AFAB5" author="Cinar H. N. & Gopinath G. & Murphy H. R. & Almeria S. & Durigan M. & Choi D. & Jang A. & Kim E. & Kim R. & Choi S. & Lee J. & Shin Y. & Lee J. & Qvarnstrom Y. & Benedict T. K. & Bishop H. S. & da Silva A." pageId="4" pageNumber="17" pagination="122" refId="ref4363" refString="Cinar H. N., Gopinath G., Murphy H. R., Almeria S., Durigan M., Choi D., Jang A., Kim E., Kim R., Choi S., Lee J., Shin Y., Lee J., Qvarnstrom Y., Benedict T. K., Bishop H. S., da Silva A. (2020) Molecular typing of Cyclospora cayetanensis in produce and clinical samples using targeted enrichment of complete mitochondrial genomes and next-generation sequencing. Parasit Vectors. 13: 122" type="journal article" year="2020">
|
|
Cinar
|
|
<emphasis id="98B2EAB47B130E06BEEFFB725CC4FAB5" box="[132,184,1259,1285]" italics="true" pageId="4" pageNumber="17">et al</emphasis>
|
|
. 2020
|
|
</bibRefCitation>
|
|
).
|
|
</paragraph>
|
|
<paragraph id="AA7936A67B130E06BEEFFA835DD3FAEA" blockId="4.[132,779,1309,1370]" pageId="4" pageNumber="17">
|
|
<emphasis id="98B2EAB47B130E06BEEFFA835DD3FAEA" bold="true" pageId="4" pageNumber="17">
|
|
<collectionCode id="CCD7AE637B130E06BEEFFA835CBCFA87" box="[132,192,1309,1335]" pageId="4" pageNumber="17">NGS</collectionCode>
|
|
shotgun, metabarcoding and commercially available diagnostic test
|
|
</emphasis>
|
|
</paragraph>
|
|
<paragraph id="AA7936A67B130E06BEC1FAEC58A2FB70" blockId="4.[132,779,1393,1938]" lastBlockId="4.[816,1463,223,1872]" pageId="4" pageNumber="17">
|
|
Progress on the improvement of emerging molecular tools to
|
|
<taxonomicName id="6DC64D257B130E06BE8DFA0A5DC8FA1E" authority="DNA" authorityName="DNA" box="[230,436,1428,1454]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="genus">
|
|
<emphasis id="98B2EAB47B130E06BE8DFA0A5D11FA1E" box="[230,365,1428,1454]" italics="true" pageId="4" pageNumber="17">Cyclospora</emphasis>
|
|
DNA
|
|
</taxonomicName>
|
|
detection has been observed but it is mostly fronted for humans (Qvarnstrom
|
|
<emphasis id="98B2EAB47B130E06BCA5FA295F77FA60" box="[718,779,1462,1488]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2018). Recent advances in modern sequencing technologies and availability of efficient software led to complete
|
|
<taxonomicName id="6DC64D257B130E06BEADF9805D03F988" box="[198,383,1566,1592]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="species" species="cayetanensis">
|
|
<emphasis id="98B2EAB47B130E06BEADF9805D03F988" box="[198,383,1566,1592]" italics="true" pageId="4" pageNumber="17">C. cayetanensis</emphasis>
|
|
</taxonomicName>
|
|
mitochondrial and apicoplast genomes (
|
|
<bibRefCitation id="CE574B577B130E06BE8BF9DE5DD4F9EB" author="Cinar H. N. & Gopinath G. & Jarvis K. & Murphy H. R." box="[224,424,1600,1627]" pageId="4" pageNumber="17" pagination="0128645" refId="ref4322" refString="Cinar H. N., Gopinath G., Jarvis K., Murphy H. R. (2015) The com- plete mitochondrial genome of the foodborne parasitic pathogen Cyclospora cayetanensis. PLoS ONE 10, e 0128645" type="journal article" year="2015">
|
|
Cinar
|
|
<emphasis id="98B2EAB47B130E06BF42F9DF5D18F9EA" box="[297,356,1600,1626]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2015
|
|
</bibRefCitation>
|
|
, Cinar
|
|
<emphasis id="98B2EAB47B130E06BF90F9DF5E4AF9EA" box="[507,566,1600,1626]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2016,
|
|
<bibRefCitation id="CE574B577B130E06BCEEF9DE5D72F9CE" author="Ogedengbe M. E. & Qvarnstrom Y. & da Silva A. J. & Arrowood M. J. & Barta J. R." pageId="4" pageNumber="17" pagination="361 - 365" refId="ref6144" refString="Ogedengbe M. E., Qvarnstrom Y., da Silva A. J., Arrowood M. J., Barta J. R. (2015) A linear mitochondrial genome of Cyclospora cayetanensis (Eimeriidae, Eucoccidiorida, Coccidiasina, Apicomplexa) suggests the ancestral start position within mitochondrial genomes of eimeriid coccidia. Int. J. Parasitol. 45: 361 - 365" type="journal article" year="2015">
|
|
Ogedengbe
|
|
<emphasis id="98B2EAB47B130E06BEEFF9FA5CB8F9CD" box="[132,196,1635,1661]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2015
|
|
</bibRefCitation>
|
|
;
|
|
<bibRefCitation id="CE574B577B130E06BF74F9FD5E3EF9CD" author="Cama V. A & Ortega Y. R." box="[287,578,1635,1661]" pageId="4" pageNumber="17" pagination="41 - 56" refId="ref4103" refString="Cama V. A and Ortega Y. R. (2018) Cyclospora cayetanensis. In Foodborne Parasites, 2 nd ed.; Ortega Y. R., Sterling C. R., Eds.; Springer: Berlin / Heidelberg, Germany, 2018; pp. 41 - 56" type="journal article" year="2018">Cama and Ortega 2018</bibRefCitation>
|
|
). The new
|
|
<collectionCode id="CCD7AE637B130E06BCBBF9FD5F77F9CD" box="[720,779,1635,1661]" pageId="4" pageNumber="17">NGS</collectionCode>
|
|
strategy used on deep sequencing platforms gains from the increasing availability, speed, and decreasing costs. In general, it is based on two approaches. The first is shotgun metagenomics, which profiles the entire microbial diversity consisting of both pathogenic and neutral microbiome of the host. This technique demands the knowledge of partial or whole
|
|
<taxonomicName id="6DC64D257B130E06BC62F8CB5EECF8DF" box="[521,656,1877,1903]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="genus">
|
|
<emphasis id="98B2EAB47B130E06BC62F8CB5EECF8DF" box="[521,656,1877,1903]" italics="true" pageId="4" pageNumber="17">Cyclospora</emphasis>
|
|
</taxonomicName>
|
|
reference genome, which is then compared to the shotgun data following quality processing, curation, and assembly datasets. The shotgun method may be promising to identify and develop novel target loci of
|
|
<taxonomicName id="6DC64D257B130E06BB40FEBA58DFFED1" authority="(Qvarnstrom et al. 2015)" baseAuthorityName="Qvarnstrom" baseAuthorityYear="2015" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="species" species="cayetanensis">
|
|
<emphasis id="98B2EAB47B130E06BB40FEBA5F17FED0" italics="true" pageId="4" pageNumber="17">C. cayetanensis</emphasis>
|
|
(
|
|
<bibRefCitation id="CE574B577B130E06BD17FED958E7FED1" author="Qvarnstrom Y. & Wei-Pridgeon Y. & Li W. & Nascimento F. S. & Bishop H. S. & Herwaldt B. L. & Moss D. M. & Nayak V. & Srinivasamoorthy G. & Sheth M." box="[892,1179,326,353]" pageId="4" pageNumber="17" pagination="6" refId="ref6894" refString="Qvarnstrom Y., Wei-Pridgeon Y., Li W., Nascimento F. S., Bishop H. S., Herwaldt B. L., Moss D. M., Nayak V., Srinivasamoorthy G., Sheth M. et al. (2015) Draft genome sequences from Cyclospora cayetanensis oocysts purified from a human stool sample. Genome Announc. 3 (6): e 01324 - 15" type="journal article" year="2015">
|
|
Qvarnstrom
|
|
<emphasis id="98B2EAB47B130E06BA7FFED9582DFED1" box="[1044,1105,327,353]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2015
|
|
</bibRefCitation>
|
|
)
|
|
</taxonomicName>
|
|
. The whole genome of
|
|
<taxonomicName id="6DC64D257B130E06BD5BFEF45F90FE33" box="[816,1004,362,388]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="species" species="cayetanensis">
|
|
<emphasis id="98B2EAB47B130E06BD5BFEF45F90FE33" box="[816,1004,362,388]" italics="true" pageId="4" pageNumber="17">C. cayetanensis</emphasis>
|
|
</taxonomicName>
|
|
is estimated to be 44 megabase pairs with ~7500 genes (
|
|
<bibRefCitation id="CE574B577B130E06BA7CFE1258ADFE17" author="Liu S. & Wang L. & Zheng H. & Xu Z. & Roellig D. M. & Li N. & Frace M. A. & Tang K. & Arrowood M. J. & Moss D. M." box="[1047,1233,396,423]" pageId="4" pageNumber="17" pagination="316" refId="ref5522" refString="Liu S., Wang L., Zheng H., Xu Z., Roellig D. M., Li N., Frace M. A., Tang K., Arrowood M. J., Moss D. M., et al. (2016) Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens. BMC Genom. 17: 316" type="journal article" year="2016">
|
|
Liu
|
|
<emphasis id="98B2EAB47B130E06BA20FE1258F4FE16" box="[1099,1160,396,422]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2016
|
|
</bibRefCitation>
|
|
).
|
|
<taxonomicName id="6DC64D257B130E06BA8DFE125911FE16" box="[1254,1389,396,422]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="genus">
|
|
<emphasis id="98B2EAB47B130E06BA8DFE125911FE16" box="[1254,1389,396,422]" italics="true" pageId="4" pageNumber="17">Cyclospora</emphasis>
|
|
</taxonomicName>
|
|
mitochondrial genome is ~6200 base pairs (bp) in length, whereas the circular apicoplast genome is ~34,000 bp and encodes complete machinery for protein synthesis. The second
|
|
<collectionCode id="CCD7AE637B130E06BDAAFD885F80FD80" box="[961,1020,534,560]" pageId="4" pageNumber="17">NGS</collectionCode>
|
|
approach is based on metabarcoding of the small ribosomal RNA subunit (18S), which targets predefined domains using specific primers. This
|
|
<collectionCode id="CCD7AE637B130E06BD5BFDE35F17FD27" box="[816,875,637,663]" pageId="4" pageNumber="17">NGS</collectionCode>
|
|
system seems to be extremely useful in terms of the development of new
|
|
<taxonomicName id="6DC64D257B130E06BA31FD3E589DFD0A" box="[1114,1249,672,698]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="genus">
|
|
<emphasis id="98B2EAB47B130E06BA31FD3E589DFD0A" box="[1114,1249,672,698]" italics="true" pageId="4" pageNumber="17">Cyclospora</emphasis>
|
|
</taxonomicName>
|
|
diagnostic assays (
|
|
<bibRefCitation id="CE574B577B130E06BD53FD5C5829FD6D" author="Qvarnstrom Y. & Wei-Pridgeon Y. & Li W. & Nascimento F. S. & Bishop H. S. & Herwaldt B. L. & Moss D. M. & Nayak V. & Srinivasamoorthy G. & Sheth M." box="[824,1109,706,733]" pageId="4" pageNumber="17" pagination="6" refId="ref6894" refString="Qvarnstrom Y., Wei-Pridgeon Y., Li W., Nascimento F. S., Bishop H. S., Herwaldt B. L., Moss D. M., Nayak V., Srinivasamoorthy G., Sheth M. et al. (2015) Draft genome sequences from Cyclospora cayetanensis oocysts purified from a human stool sample. Genome Announc. 3 (6): e 01324 - 15" type="journal article" year="2015">
|
|
Qvarnstrom
|
|
<emphasis id="98B2EAB47B130E06BDBBFD5D5871FD6C" box="[976,1037,706,732]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2015
|
|
</bibRefCitation>
|
|
;
|
|
<bibRefCitation id="CE574B577B130E06BA0FFD5C5903FD6C" author="Nascimento F. & Wei-Pridgeon Y. & Arrowood M. J. & Moss D. & da Silva A. J. & Talundzic E. & Qvarnstrom Y." box="[1124,1407,706,732]" pageId="4" pageNumber="17" pagination="23 - 26" refId="ref6011" refString="Nascimento F. S, Wei-Pridgeon Y., Arrowood M. J., Moss D., da Silva A. J., Talundzic E., Qvarnstrom Y. (2016) Evaluation of library preparation methods for Illumina next generation sequencing of small amounts of DNA from foodborne parasites. J. Microbiol. Methods. 130: 23 - 26" type="journal article" year="2016">
|
|
Nascimento
|
|
<emphasis id="98B2EAB47B130E06BA91FD5D594BFD6C" box="[1274,1335,706,732]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2016
|
|
</bibRefCitation>
|
|
;
|
|
<bibRefCitation id="CE574B577B130E06BBE5FD5C5FC9FCB0" author="Liu S. & Wang L. & Zheng H. & Xu Z. & Roellig D. M. & Li N. & Frace M. A. & Tang K. & Arrowood M. J. & Moss D. M." pageId="4" pageNumber="17" pagination="316" refId="ref5522" refString="Liu S., Wang L., Zheng H., Xu Z., Roellig D. M., Li N., Frace M. A., Tang K., Arrowood M. J., Moss D. M., et al. (2016) Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens. BMC Genom. 17: 316" type="journal article" year="2016">
|
|
Liu
|
|
<emphasis id="98B2EAB47B130E06BD5BFD785F11FD4F" box="[816,877,741,767]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2016
|
|
</bibRefCitation>
|
|
). Cinar and coworkers described
|
|
<collectionCode id="CCD7AE637B130E06BB2FFD7B5903FD4F" box="[1348,1407,741,767]" pageId="4" pageNumber="17">NGS</collectionCode>
|
|
molecular typing of
|
|
<taxonomicName id="6DC64D257B130E06BA6AFC9658C0FC91" box="[1025,1212,776,802]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="species" species="cayetanensis">
|
|
<emphasis id="98B2EAB47B130E06BA6AFC9658C0FC91" box="[1025,1212,776,802]" italics="true" pageId="4" pageNumber="17">C. cayetanensis</emphasis>
|
|
</taxonomicName>
|
|
identifying potential genomic markers such as single nucleotide polymorphisms (
|
|
<collectionCode id="CCD7AE637B130E06BDFDFCD35FB3FCD7" box="[918,975,845,871]" country="Malaysia" httpUri="http://biocol.org/urn:lsid:biocol.org:col:15856" lsid="urn:lsid:biocol.org:col:15856" name="Sabah Parks" pageId="4" pageNumber="17" type="Herbarium">SNP</collectionCode>
|
|
) and insertion-deletions that could theoretically be used for
|
|
<taxonomicName id="6DC64D257B130E06BA77FCF158DFFC39" box="[1052,1187,879,905]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="genus">
|
|
<emphasis id="98B2EAB47B130E06BA77FCF158DFFC39" box="[1052,1187,879,905]" italics="true" pageId="4" pageNumber="17">Cyclospora</emphasis>
|
|
</taxonomicName>
|
|
detection and pathogen subtyping in clinical samples (
|
|
<bibRefCitation id="CE574B577B130E06BAF8FC0F5922FC1C" author="Cinar H. N. & Gopinath G. & Murphy H. R. & Almeria S. & Durigan M. & Choi D. & Jang A. & Kim E. & Kim R. & Choi S. & Lee J. & Shin Y. & Lee J. & Qvarnstrom Y. & Benedict T. K. & Bishop H. S. & da Silva A." box="[1171,1374,913,940]" pageId="4" pageNumber="17" pagination="122" refId="ref4363" refString="Cinar H. N., Gopinath G., Murphy H. R., Almeria S., Durigan M., Choi D., Jang A., Kim E., Kim R., Choi S., Lee J., Shin Y., Lee J., Qvarnstrom Y., Benedict T. K., Bishop H. S., da Silva A. (2020) Molecular typing of Cyclospora cayetanensis in produce and clinical samples using targeted enrichment of complete mitochondrial genomes and next-generation sequencing. Parasit Vectors. 13: 122" type="journal article" year="2020">
|
|
Cinar
|
|
<emphasis id="98B2EAB47B130E06BAB6FC0C5964FC1B" box="[1245,1304,913,939]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2020
|
|
</bibRefCitation>
|
|
). These promising results were obtained by typing the mitochondrial genome. It was suggested that the diversity of
|
|
<taxonomicName id="6DC64D257B130E06BD3AFC645876FBA3" box="[849,1034,1018,1044]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="species" species="cayetanensis">
|
|
<emphasis id="98B2EAB47B130E06BD3AFC645876FBA3" box="[849,1034,1018,1044]" italics="true" pageId="4" pageNumber="17">C. cayetanensis</emphasis>
|
|
</taxonomicName>
|
|
and could be used to link outbreaks or even single infection cases to a source. Multicopy and linear mitochondrial genomic sequences observed in
|
|
<taxonomicName id="6DC64D257B130E06BD3AFBFF5870FBCA" box="[849,1036,1121,1147]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="species" species="cayetanensis">
|
|
<emphasis id="98B2EAB47B130E06BD3AFBFF5870FBCA" box="[849,1036,1121,1147]" italics="true" pageId="4" pageNumber="17">C. cayetanensis</emphasis>
|
|
</taxonomicName>
|
|
may also be used for the detection and genotyping of other
|
|
<taxonomicName id="6DC64D257B130E06BA1AFB1D5921FB2D" box="[1137,1373,1155,1181]" pageId="4" pageNumber="17">
|
|
<emphasis id="98B2EAB47B130E06BA1AFB1D5884FB2D" box="[1137,1272,1155,1181]" italics="true" pageId="4" pageNumber="17">Cyclospora</emphasis>
|
|
species
|
|
</taxonomicName>
|
|
(
|
|
<bibRefCitation id="CE574B577B130E06BB1FFB1D5FCCFB71" author="Cinar H. N. & Gopinath G. & Jarvis K. & Murphy H. R." pageId="4" pageNumber="17" pagination="0128645" refId="ref4322" refString="Cinar H. N., Gopinath G., Jarvis K., Murphy H. R. (2015) The com- plete mitochondrial genome of the foodborne parasitic pathogen Cyclospora cayetanensis. PLoS ONE 10, e 0128645" type="journal article" year="2015">
|
|
Cinar
|
|
<emphasis id="98B2EAB47B130E06BD5BFB395F17FB70" box="[816,875,1190,1216]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2015
|
|
</bibRefCitation>
|
|
; Qvarnstrom
|
|
<emphasis id="98B2EAB47B130E06BA3BFB3958F7FB70" box="[1104,1163,1190,1216]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2018).
|
|
</paragraph>
|
|
<paragraph id="AA7936A67B130E06BD3DFB565FEFF8E0" blockId="4.[816,1463,223,1872]" pageId="4" pageNumber="17">
|
|
The development of rapid diagnostic molecular tests has improved the detection of various protozoan pathogens thanks to higher throughput capacity (
|
|
<bibRefCitation id="CE574B577B130E06BBE8FA935829FAFA" author="Verweij J. J. & Stensvold R. C." pageId="4" pageNumber="17" pagination="371 - 418" refId="ref7468" refString="Verweij J. J. and Stensvold R. C. (2014) Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections. Clin Microbiol Rev. 27: 371 - 418" type="journal article" year="2014">Verweij and Verweij 2014</bibRefCitation>
|
|
). Besides user-friendly software and equipment independence, the ultimate goal of such tests should be better affordability, sensitivity and specificity. Currently, the BioFire FilmArray panel is the only commercially available product capable of detecting
|
|
<taxonomicName id="6DC64D257B130E06BDC9FA435825FA46" box="[930,1113,1501,1527]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="species" species="cayetanensis">
|
|
<emphasis id="98B2EAB47B130E06BDC9FA435825FA46" box="[930,1113,1501,1527]" italics="true" pageId="4" pageNumber="17">C. cayetanensis</emphasis>
|
|
</taxonomicName>
|
|
in addition to 22 enteropathogenic agents (including four protozoan species). Buss and coworkers described the sensitivity and specificity of this test during a cyclosporiasis outbreak in the
|
|
<collectingCountry id="D2D176367B130E06BB15F9DA59CBF9EE" box="[1406,1463,1604,1630]" name="United States of America" pageId="4" pageNumber="17">USA</collectingCountry>
|
|
(
|
|
<bibRefCitation id="CE574B577B130E06BD52F9F9587DF931" author="Buss S. N. & Alter R. & Iwen P. C. & Fey P. D." box="[825,1025,1638,1665]" pageId="4" pageNumber="17" pagination="3909" refId="ref3984" refString="Buss S. N., Alter R., Iwen P. C., Fey P. D. (2013) Implications of culture-independent panel-based detection of Cyclospora cayetanensis. J. Clin. Microbiol. 51: 3909" type="journal article" year="2013">
|
|
Buss
|
|
<emphasis id="98B2EAB47B130E06BD17F9F95FC4F930" box="[892,952,1638,1664]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2013
|
|
</bibRefCitation>
|
|
). In another study, over one and half thousand clinical stool samples were analyzed, showing that the sensitivity and specificity of this test for
|
|
<taxonomicName id="6DC64D257B130E06BD5BF9515F97F958" box="[816,1003,1743,1769]" family="Eimeriidae" genus="Cyclospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="17" phylum="Miozoa" rank="species" species="cayetanensis">
|
|
<emphasis id="98B2EAB47B130E06BD5BF9515F97F958" box="[816,1003,1743,1769]" italics="true" pageId="4" pageNumber="17">C. cayetanensis</emphasis>
|
|
</taxonomicName>
|
|
was 100% (
|
|
<bibRefCitation id="CE574B577B130E06BAE9F9505936F959" author="Buss S. N. & Leber A. & Chapin K. & Fey P. D. & Bankowski M. J. & Jones M. K. & Rogatcheva M. & Kanack K. J. & Bourzac K. M." box="[1154,1354,1742,1769]" pageId="4" pageNumber="17" pagination="915 - 925" refId="ref4026" refString="Buss S. N., Leber A., Chapin K., Fey P. D., Bankowski M. J., Jones M. K., Rogatcheva M., Kanack K. J., Bourzac K. M. (2015) Multicenter evaluation of the BioFire FilmArray Gastrointes- tinal Panel for etiologic diagnosis of infectious gastroenteritis. J. Clin. Microbiol. 53: 915 - 925" type="journal article" year="2015">
|
|
Buss
|
|
<emphasis id="98B2EAB47B130E06BAADF951597EF958" box="[1222,1282,1742,1768]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2015
|
|
</bibRefCitation>
|
|
; Murphy
|
|
<emphasis id="98B2EAB47B130E06BD5BF96F5F11F8BB" box="[816,877,1777,1803]" italics="true" pageId="4" pageNumber="17">et al.</emphasis>
|
|
2019). Up to now, no reports were published on the use of this commercial test to analyze samples from animals.
|
|
</paragraph>
|
|
</subSubSection>
|
|
</treatment>
|
|
</document> |