316 lines
35 KiB
XML
316 lines
35 KiB
XML
<document id="8584ED0190137389504B1671FD31E880" ID-DOI="10.12651/JSR.2020.9.3.210" ID-ISSN="2713-8615" ID-Zenodo-Dep="13140633" IM.bibliography_approvedBy="valdenar" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="valdenar" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="valdenar" checkinTime="1722404678219" checkinUser="felipe" docAuthor="Bae, Eun Hee, Kang, Jae-Shin & Park, Chong-Sung" docDate="2020" docId="161ADE5B846EFFEAFCE24E242AAC8BB8" docLanguage="en" docName="JSpeciesRes.9.3.210-217.pdf" docOrigin="Journal of Species Research 9 (3)" docSource="http://dx.doi.org/10.5281/zenodo.13140644" docStyle="DocumentStyle:4D398BFF45FF8CA9E08F546BB06AE372.2:JSpeciesRes.2012-.journal_article" docStyleId="4D398BFF45FF8CA9E08F546BB06AE372" docStyleName="JSpeciesRes.2012-.journal_article" docStyleVersion="2" docTitle="Microseira wollei G. B. McGregor and Sendall ex Kennis 2017" docType="treatment" docVersion="1" lastPageNumber="215" masterDocId="EA23A623846DFFEFFFA548732E3D8E04" masterDocTitle="New report on cyanophyte in Korea, Microseira wollei (Farlow ex Gomont) G. B. McGregor and Sendall ex Kennis (Oscillatoriaceae)" masterLastPageNumber="217" masterPageNumber="210" pageNumber="213" updateTime="1728065080696" updateUser="valdenar" zenodo-license-document="CC-BY-4.0">
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<mods:title id="A22EA27BC38626677549BB82D1935272">New report on cyanophyte in Korea, Microseira wollei (Farlow ex Gomont) G. B. McGregor and Sendall ex Kennis (Oscillatoriaceae)</mods:title>
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<mods:namePart id="50AF560689CF6A8296AEC272B810E70E">Bae, Eun Hee</mods:namePart>
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<mods:namePart id="EB62DB1051F8D0A1EF57D0E7D94E15EC">Kang, Jae-Shin</mods:namePart>
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<mods:nameIdentifier id="A39A8E50DD48C5A065ABC69902EC628D" type="email">diatom@korea.kr</mods:nameIdentifier>
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<mods:namePart id="AA5AD239079266298437A6315F76D40C">Park, Chong-Sung</mods:namePart>
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<mods:title id="16F305642346FC6D3A72477CB277EF38">Journal of Species Research</mods:title>
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<mods:part id="BFB1B4B6495D6524B59A4B2024289E51">
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<mods:date id="03E1035259D909F52B5764E8DE016BD3">2020</mods:date>
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<mods:number id="E85F7FB84115FF14177522556A984B69">2020-07-29</mods:number>
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<treatment id="161ADE5B846EFFEAFCE24E242AAC8BB8" LSID="urn:lsid:plazi:treatment:161ADE5B846EFFEAFCE24E242AAC8BB8" httpUri="http://treatment.plazi.org/id/161ADE5B846EFFEAFCE24E242AAC8BB8" lastPageId="5" lastPageNumber="215" pageId="3" pageNumber="213">
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<subSubSection id="D6A93CC6846EFFECFCE24E242ABE888A" pageId="3" pageNumber="213" type="nomenclature">
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<paragraph id="9E0C6F4D846EFFECFCE24E242ABE888A" blockId="3.[839,1444,1525,1678]" pageId="3" pageNumber="213">
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<taxonomicName id="59B314CE846EFFECFCE24E242ABE888A" authority="(Farlow ex Gomont) G. B. McGregor and Sendall ex Kennis 2017" authorityName="G. B. McGregor and Sendall ex Kennis" authorityYear="2017" baseAuthorityName="Farlow ex Gomont" class="Cyanobacteriia" family="Aerosakkonemataceae" genus="Microseira" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Cyanobacteriales" pageId="3" pageNumber="213" phylum="Cyanobacteria" rank="species" species="wollei">
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<emphasis id="ACC7B35F846EFFECFCE24E242A31886A" box="[839,1036,1622,1646]" italics="true" pageId="3" pageNumber="213">Microseira wollei</emphasis>
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(Farlow ex Gomont) G.B.McGregor and Sendall ex Kennis 2017
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</taxonomicName>
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</paragraph>
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</subSubSection>
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<subSubSection id="D6A93CC6846EFFECFCE24EC42AAC8914" pageId="3" pageNumber="213" type="reference_group">
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<paragraph id="9E0C6F4D846EFFECFCE24EC42AAC8914" blockId="3.[839,1444,1719,1969]" pageId="3" pageNumber="213">
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Basionym:
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<taxonomicName id="59B314CE846EFFECFC644EC42DFE88EB" authority="Farlow ex Gomont 1892 Homotypic" authorityName="Farlow ex Gomont" authorityYear="1892" class="Cyanophyceae" family="Scytonemataceae" genus="Plectonema" kingdom="Bacteria" order="Nostocales" pageId="3" pageNumber="213" phylum="Cyanobacteria" rank="species" species="wollei">
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<emphasis id="ACC7B35F846EFFECFC644EC42ABA88CB" box="[961,1159,1719,1743]" italics="true" pageId="3" pageNumber="213">Plectonema wollei</emphasis>
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Farlow ex
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<bibRefCitation id="FA2212BC846EFFECFAA54EC42BAE88CB" author="Gomont, M." box="[1280,1427,1719,1743]" pageId="3" pageNumber="213" pagination="1 - 7" refId="ref3538" refString="Gomont, M. 1892 ' 1893 '. Monographie des Oscillariees (Nostocacees Homocystees). Deuxieme partie. - Lyngbyees. Annales des Sciences Naturelles, Botanique, Serie 7 16: 91 - 264, pls 1 - 7." type="book chapter" year="1892">Gomont 1892</bibRefCitation>
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Homotypic
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</taxonomicName>
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synonym:
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<taxonomicName id="59B314CE846EFFECFBE64EA42AAC8914" authority="(Farlow ex Gomont) Speziale and Dyck 1992" authorityName="Speziale and Dyck" authorityYear="1992" baseAuthorityName="Gomont" class="Cyanophyceae" family="Oscillatoriaceae" genus="Lyngbya" kingdom="Bacteria" order="Nostocales" pageId="3" pageNumber="213" phylum="Cyanobacteria" rank="species" species="wollei">
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<emphasis id="ACC7B35F846EFFECFBE64EA42AD288EB" box="[1091,1263,1751,1775]" italics="true" pageId="3" pageNumber="213">Lyngbya wollei</emphasis>
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(Farlow ex Gomont)
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<bibRefCitation id="FA2212BC846EFFECFC284E842AAC8914" author="Speziale, B. J. & L. A. Dyck" box="[909,1169,1783,1808]" pageId="3" pageNumber="213" pagination="693 - 706" refId="ref4159" refString="Speziale, B. J. and L. A. Dyck. 1992. Lyngbya infestations: comparative taxonomy of Lyngbya wollei comb. nov. (Cyanobacteria). Journal of Phycology 28 (5): 693 - 706." type="journal article" year="1992">Speziale and Dyck 1992</bibRefCitation>
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</taxonomicName>
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</paragraph>
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||
</subSubSection>
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||
<subSubSection id="D6A93CC6846EFFECFCE24F6B2ADF8934" box="[839,1250,1815,1840]" pageId="3" pageNumber="213" type="materials_examined">
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<paragraph id="9E0C6F4D846EFFECFCE24F6B2ADF8934" blockId="3.[839,1444,1719,1969]" box="[839,1250,1815,1840]" pageId="3" pageNumber="213">
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Type specimen: 945406 (
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<typeStatus id="4108D1EF846EFFECFBF44F6B2A958934" box="[1105,1192,1816,1840]" pageId="3" pageNumber="213" type="syntype">syntype</typeStatus>
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, NY)
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</paragraph>
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</subSubSection>
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<subSubSection id="D6A93CC6846EFFEBFCE24F4B2CB98845" lastPageId="4" lastPageNumber="214" pageId="3" pageNumber="213" type="description">
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<paragraph id="9E0C6F4D846EFFEBFCE24F4B2CB98845" blockId="3.[839,1444,1719,1969]" lastBlockId="4.[144,749,1544,1988]" lastPageId="4" lastPageNumber="214" pageId="3" pageNumber="213">
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Habitat: The filaments of
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<taxonomicName id="59B314CE846EFFECFBC44F4A2B1D8954" authorityName="G. B. McGregor and Sendall ex Kennis" authorityYear="2017" baseAuthorityName="Farlow ex Gomont" box="[1121,1312,1848,1872]" class="Cyanobacteriia" family="Aerosakkonemataceae" genus="Microseira" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Cyanobacteriales" pageId="3" pageNumber="213" phylum="Cyanobacteria" rank="species" species="wollei">
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<emphasis id="ACC7B35F846EFFECFBC44F4A2B1D8954" box="[1121,1312,1848,1872]" italics="true" pageId="3" pageNumber="213">Microseira wollei</emphasis>
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</taxonomicName>
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were found in sublittoral habitat of a freshwater river entwined in the basal part of aquatic macrophytes or plants.
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<materialsCitation id="2EDB6510846EFFEBFCE24FEA2CBC8845" county="Nakdong River" lastPageId="4" lastPageNumber="214" latitude="35.858776" location="Collection" longLatPrecision="1" longitude="128.38972" municipality="Daegu-city" pageId="3" pageNumber="213" specimenCount="1">
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<location id="9B6C3996846EFFECFCE24FEA2D8B89B5" LSID="urn:lsid:plazi:treatment:161ADE5B846EFFEAFCE24E242AAC8BB8:9B6C3996846EFFECFCE24FEA2D8B89B5" box="[839,950,1945,1969]" county="Nakdong River" latitude="35.858776" longLatPrecision="1" longitude="128.38972" municipality="Daegu-city" name="Collection" pageId="3" pageNumber="213">Collection</location>
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site:
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<location id="9B6C3996846EFFECFC554FEA2A2489B5" LSID="urn:lsid:plazi:treatment:161ADE5B846EFFEAFCE24E242AAC8BB8:9B6C3996846EFFECFC554FEA2A2489B5" box="[1008,1049,1945,1969]" county="Nakdong River" latitude="35.858776" longLatPrecision="1" longitude="128.38972" municipality="Daegu-city" name="The" pageId="3" pageNumber="213">The</location>
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riverside of
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<quantity id="594BC2A8846EFFECFB044FEA2AEA89B5" box="[1185,1239,1945,1969]" metricMagnitude="3" metricUnit="m" metricValue="7.0" pageId="3" pageNumber="213" unit="km" value="7.0">7 km</quantity>
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upper stream from
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<location id="9B6C39968469FFEBFF354E7B2F448824" LSID="urn:lsid:plazi:treatment:161ADE5B846EFFEAFCE24E242AAC8BB8:9B6C39968469FFEBFF354E7B2F448824" box="[144,377,1544,1568]" county="Nakdong River" latitude="35.858776" longLatPrecision="1" longitude="128.38972" municipality="Daegu-city" name="Gangjeong-Goryeong" pageId="4" pageNumber="214">Gangjeong-Goryeong</location>
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weir at
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<location id="9B6C39968469FFEBFE724E7B2C598824" LSID="urn:lsid:plazi:treatment:161ADE5B846EFFEAFCE24E242AAC8BB8:9B6C39968469FFEBFE724E7B2C598824" box="[471,612,1544,1568]" county="Nakdong River" latitude="35.858776" longLatPrecision="1" longitude="128.38972" municipality="Daegu-city" name="Dalsung-gun" pageId="4" pageNumber="214">Dalsung-gun</location>
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,
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<collectingMunicipality id="7E68F5378469FFEBFDD44E7B2CD48824" box="[625,745,1544,1568]" pageId="4" pageNumber="214">Daegu-city</collectingMunicipality>
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,
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<collectingCounty id="776D17C18469FFEBFF354E5B2F0E8844" box="[144,307,1576,1600]" pageId="4" pageNumber="214">Nakdong River</collectingCounty>
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(
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35°51
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<emphasis id="ACC7B35F8469FFEBFE244E542FBA8845" bold="true" box="[385,391,1575,1601]" italics="true" pageId="4" pageNumber="214">ʹ</emphasis>
|
||
31.6
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<emphasis id="ACC7B35F8469FFEBFE104E542F828845" bold="true" box="[437,447,1575,1601]" italics="true" pageId="4" pageNumber="214">ʺ</emphasis>
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N
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</geoCoordinate>
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<geoCoordinate id="FB87098A8469FFEBFE7C4E5A2C458845" box="[473,632,1575,1601]" degrees="128" direction="east" minutes="23" orientation="longitude" pageId="4" pageNumber="214" precision="1" seconds="23.0" value="128.38972">
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128°23
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<emphasis id="ACC7B35F8469FFEBFD834E542C118845" bold="true" box="[550,556,1575,1601]" italics="true" pageId="4" pageNumber="214">ʹ</emphasis>
|
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23.0
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<emphasis id="ACC7B35F8469FFEBFDFE4E542C588845" bold="true" box="[603,613,1575,1601]" italics="true" pageId="4" pageNumber="214">ʺ</emphasis>
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E
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</geoCoordinate>
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)
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</materialsCitation>
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.
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</paragraph>
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</subSubSection>
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<caption id="CACC3FC58469FFEBFF344D2F2A408BC6" ID-DOI="http://doi.org/10.5281/zenodo.13140643" ID-Zenodo-Dep="13140643" httpUri="https://zenodo.org/record/13140643/files/figure.png" pageId="4" pageNumber="214" startId="4.[145,178,1372,1394]" targetBox="[253,1285,261,1339]" targetPageId="4" targetType="figure">
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<paragraph id="9E0C6F4D8469FFEBFF344D2F2A408BC6" blockId="4.[144,1414,1372,1474]" pageId="4" pageNumber="214">
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<emphasis id="ACC7B35F8469FFEBFF344D2F2EF38B75" bold="true" box="[145,206,1372,1394]" pageId="4" pageNumber="214">Fig. 4.</emphasis>
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Phylogenetic tree based on the 16S rRNA gene partial sequences aligned 1,267 bp showing the genetic position of
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<taxonomicName id="59B314CE8469FFEBFB414D2F2BB88B75" authorityName="G. B. McGregor and Sendall ex Kennis" authorityYear="2017" baseAuthorityName="Farlow ex Gomont" box="[1252,1413,1372,1393]" class="Cyanobacteriia" family="Aerosakkonemataceae" genus="Microseira" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Cyanobacteriales" pageId="4" pageNumber="214" phylum="Cyanobacteria" rank="species" species="wollei">
|
||
<emphasis id="ACC7B35F8469FFEBFB414D2F2BB88B75" box="[1252,1413,1372,1393]" italics="true" pageId="4" pageNumber="214">Microseira wollei</emphasis>
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||
</taxonomicName>
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||
of Korea along with other geographical populations and related taxa with some taxonomic treatment of
|
||
<taxonomicName id="59B314CE8469FFEBFBEE4D042A9C8B8F" authorityName="G. B. McGregor and Sendall ex Kennis" authorityYear="2017" baseAuthorityName="Farlow ex Gomont" box="[1099,1185,1398,1420]" class="Cyanobacteriia" family="Aerosakkonemataceae" genus="Microseira" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Cyanobacteriales" pageId="4" pageNumber="214" phylum="Cyanobacteria" rank="species" species="wollei">
|
||
<emphasis id="ACC7B35F8469FFEBFBEE4D042A9C8B8F" box="[1099,1185,1398,1420]" italics="true" pageId="4" pageNumber="214">M. wollei</emphasis>
|
||
</taxonomicName>
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||
. Numbers indicate bootstrap value (>50%) from 1,000 replicates maximum parsimony (MP), maximum likelihood (ML), neighbor-joining (NJ) analysis respectively. GenBank accession numbers are shown in parentheses (scale bar: 0.02 nucleotide substitutions per site).
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||
</paragraph>
|
||
</caption>
|
||
<subSubSection id="D6A93CC68469FFEBFF354E3A2F488966" pageId="4" pageNumber="214" type="materials_examined">
|
||
<paragraph id="9E0C6F4D8469FFEBFF354E3A2F488966" blockId="4.[144,749,1544,1988]" pageId="4" pageNumber="214">
|
||
Specimens: NIBRCY0000001021 (collected by E.H. Bae, no. EHB20200513-1,
|
||
<geoCoordinate id="FB87098A8469FFEBFE174E1A2C7E8884" box="[434,579,1639,1665]" degrees="35" direction="north" minutes="51" orientation="latitude" pageId="4" pageNumber="214" precision="1" seconds="31.6" value="35.858776">
|
||
35°51
|
||
<emphasis id="ACC7B35F8469FFEBFE574E142FC58885" bold="true" box="[498,504,1639,1665]" italics="true" pageId="4" pageNumber="214">ʹ</emphasis>
|
||
31.6
|
||
<emphasis id="ACC7B35F8469FFEBFD834E142C0D8885" bold="true" box="[550,560,1639,1665]" italics="true" pageId="4" pageNumber="214">ʺ</emphasis>
|
||
N
|
||
</geoCoordinate>
|
||
<geoCoordinate id="FB87098A8469FFEBFDEF4E1A2CD58884" box="[586,744,1639,1665]" degrees="128" direction="east" minutes="23" orientation="longitude" pageId="4" pageNumber="214" precision="1" seconds="23.0" value="128.38972">
|
||
128°23
|
||
<emphasis id="ACC7B35F8469FFEBFD324E142CA08885" bold="true" box="[663,669,1639,1665]" italics="true" pageId="4" pageNumber="214">ʹ</emphasis>
|
||
23.0
|
||
<emphasis id="ACC7B35F8469FFEBFD694E142CEB8885" bold="true" box="[716,726,1639,1665]" italics="true" pageId="4" pageNumber="214">ʺ</emphasis>
|
||
E
|
||
</geoCoordinate>
|
||
,
|
||
<date id="EA0D498D8469FFEBFF354EFA2F1988A5" box="[144,292,1673,1698]" pageId="4" pageNumber="214" value="2020-05-13">13 May 2020</date>
|
||
), NIBRCY0000001022 (collected by E.H. Bae, no. EHB20200513-2,
|
||
<geoCoordinate id="FB87098A8469FFEBFE174EDA2C7E88C5" box="[434,579,1704,1730]" degrees="35" direction="north" minutes="51" orientation="latitude" pageId="4" pageNumber="214" precision="1" seconds="31.6" value="35.858776">
|
||
35°51
|
||
<emphasis id="ACC7B35F8469FFEBFE574EDB2FC588C6" bold="true" box="[498,504,1704,1730]" italics="true" pageId="4" pageNumber="214">ʹ</emphasis>
|
||
31.6
|
||
<emphasis id="ACC7B35F8469FFEBFD834EDB2C0D88C6" bold="true" box="[550,560,1704,1730]" italics="true" pageId="4" pageNumber="214">ʺ</emphasis>
|
||
N
|
||
</geoCoordinate>
|
||
<geoCoordinate id="FB87098A8469FFEBFDEF4ED92CD588C5" box="[586,744,1704,1730]" degrees="128" direction="east" minutes="23" orientation="longitude" pageId="4" pageNumber="214" precision="1" seconds="23.0" value="128.38972">
|
||
128°23
|
||
<emphasis id="ACC7B35F8469FFEBFD324EDB2CA088C6" bold="true" box="[663,669,1704,1730]" italics="true" pageId="4" pageNumber="214">ʹ</emphasis>
|
||
23.0
|
||
<emphasis id="ACC7B35F8469FFEBFD694EDB2CEB88C6" bold="true" box="[716,726,1704,1730]" italics="true" pageId="4" pageNumber="214">ʺ</emphasis>
|
||
E
|
||
</geoCoordinate>
|
||
,
|
||
<date id="EA0D498D8469FFEBFF354EB92F1988E6" box="[144,292,1738,1762]" pageId="4" pageNumber="214" value="2020-05-13">13 May 2020</date>
|
||
), NIBRCY0000001023 (collected by E.H. Bae, no. EHB20200513-3,
|
||
<geoCoordinate id="FB87098A8469FFEBFE174E992C7E8906" box="[434,579,1768,1794]" degrees="35" direction="north" minutes="51" orientation="latitude" pageId="4" pageNumber="214" precision="1" seconds="31.6" value="35.858776">
|
||
35°51
|
||
<emphasis id="ACC7B35F8469FFEBFE574E9B2FC58906" bold="true" box="[498,504,1768,1794]" italics="true" pageId="4" pageNumber="214">ʹ</emphasis>
|
||
31.6
|
||
<emphasis id="ACC7B35F8469FFEBFD834E9B2C0D8906" bold="true" box="[550,560,1768,1794]" italics="true" pageId="4" pageNumber="214">ʺ</emphasis>
|
||
N
|
||
</geoCoordinate>
|
||
<geoCoordinate id="FB87098A8469FFEBFDEF4E992CD58906" box="[586,744,1768,1794]" degrees="128" direction="east" minutes="23" orientation="longitude" pageId="4" pageNumber="214" precision="1" seconds="23.0" value="128.38972">
|
||
128°23
|
||
<emphasis id="ACC7B35F8469FFEBFD324E9B2CA08906" bold="true" box="[663,669,1768,1794]" italics="true" pageId="4" pageNumber="214">ʹ</emphasis>
|
||
23.0
|
||
<emphasis id="ACC7B35F8469FFEBFD694E9B2CEB8906" bold="true" box="[716,726,1768,1794]" italics="true" pageId="4" pageNumber="214">ʺ</emphasis>
|
||
E
|
||
</geoCoordinate>
|
||
,
|
||
<date id="EA0D498D8469FFEBFF354F792F198926" box="[144,292,1802,1827]" pageId="4" pageNumber="214" value="2020-05-13">13 May 2020</date>
|
||
), NIBRCY0000001024 (collected by E.H. Bae, no. EHB20200616-1,
|
||
<geoCoordinate id="FB87098A8469FFEBFE174F592C7E8946" box="[434,579,1833,1859]" degrees="35" direction="north" minutes="51" orientation="latitude" pageId="4" pageNumber="214" precision="1" seconds="31.6" value="35.858776">
|
||
35°51
|
||
<emphasis id="ACC7B35F8469FFEBFE574F5A2FC58947" bold="true" box="[498,504,1833,1859]" italics="true" pageId="4" pageNumber="214">ʹ</emphasis>
|
||
31.6
|
||
<emphasis id="ACC7B35F8469FFEBFD834F5A2C0D8947" bold="true" box="[550,560,1833,1859]" italics="true" pageId="4" pageNumber="214">ʺ</emphasis>
|
||
N
|
||
</geoCoordinate>
|
||
<geoCoordinate id="FB87098A8469FFEBFDEF4F582CD58946" box="[586,744,1833,1859]" degrees="128" direction="east" minutes="23" orientation="longitude" pageId="4" pageNumber="214" precision="1" seconds="23.0" value="128.38972">
|
||
128°23
|
||
<emphasis id="ACC7B35F8469FFEBFD324F5A2CA08947" bold="true" box="[663,669,1833,1859]" italics="true" pageId="4" pageNumber="214">ʹ</emphasis>
|
||
23.0
|
||
<emphasis id="ACC7B35F8469FFEBFD694F5A2CEB8947" bold="true" box="[716,726,1833,1859]" italics="true" pageId="4" pageNumber="214">ʺ</emphasis>
|
||
E
|
||
</geoCoordinate>
|
||
,
|
||
<date id="EA0D498D8469FFEBFF354F382F228967" box="[144,287,1867,1891]" pageId="4" pageNumber="214" value="2020-06-16">16 June 2020</date>
|
||
) in KB.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="D6A93CC68469FFEAFF354F182AAC8BB8" lastPageId="5" lastPageNumber="215" pageId="4" pageNumber="214" type="description">
|
||
<paragraph id="9E0C6F4D8469FFEBFF354F182C6D89C0" blockId="4.[144,749,1544,1988]" pageId="4" pageNumber="214">
|
||
Habit:
|
||
<taxonomicName id="59B314CE8469FFEBFF784F1F2F9F8987" authorityName="G. B. McGregor and Sendall ex Kennis" authorityYear="2017" baseAuthorityName="Farlow ex Gomont" box="[221,418,1899,1923]" class="Cyanobacteriia" family="Aerosakkonemataceae" genus="Microseira" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Cyanobacteriales" pageId="4" pageNumber="214" phylum="Cyanobacteria" rank="species" species="wollei">
|
||
<emphasis id="ACC7B35F8469FFEBFF784F1F2F9F8987" box="[221,418,1899,1923]" italics="true" pageId="4" pageNumber="214">Microseira wollei</emphasis>
|
||
</taxonomicName>
|
||
was identified as remarkably large-celled, greenish black filamentous, mat-forming characteristics, observed with naked eyes.
|
||
</paragraph>
|
||
<paragraph id="9E0C6F4D8469FFEBFC8C4E7B2D8B8824" blockId="4.[809,950,1544,1568]" box="[809,950,1544,1568]" pageId="4" pageNumber="214">
|
||
<heading id="C544D8218469FFEBFC8C4E7B2D8B8824" bold="true" box="[809,950,1544,1568]" fontSize="10" level="2" pageId="4" pageNumber="214" reason="4">
|
||
<emphasis id="ACC7B35F8469FFEBFC8C4E7B2D8B8824" bold="true" box="[809,950,1544,1568]" pageId="4" pageNumber="214">Morphology</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="9E0C6F4D8469FFEAFC9A4E3A2CC88ABE" blockId="4.[809,1414,1609,1988]" lastBlockId="5.[174,757,1186,1210]" lastPageId="5" lastPageNumber="215" pageId="4" pageNumber="214">
|
||
Thallus a prostrate, greenish blue-black tough mat of densely entangled filaments (
|
||
<figureCitation id="068873C88469FFEBFBFC4E1A2AAB8885" box="[1113,1174,1641,1665]" captionStart="Fig" captionStartId="1.[176,210,1123,1145]" captionTargetBox="[273,1333,257,1077]" captionTargetId="figure-352@1.[273,1333,257,1096]" captionTargetPageId="1" captionText="Fig. 1. Collection site and specimen information of Microseira wollei. A. Collection site near Gangjeong-Goryung weir in the Nak- dong river. B. Natural form of Microseira wollei. C. Dried specimens deposited in KB, NIBRCY0000001021 (13 May 2020). D. NI- BRCY0000001024 (16 Jun 2020)." figureDoi="http://doi.org/10.5281/zenodo.13140635" httpUri="https://zenodo.org/record/13140635/files/figure.png" pageId="4" pageNumber="214">Fig. 1</figureCitation>
|
||
). Filaments straight to variously contorted, and infrequently false-branched. An individual filament consists of an uniseriate file of discoids to cylindrical cells. Cell division occurs throughout the length of the filament and apex is flat at immediately after trichome division. Sheath colorless, hyaline, when old distinctly thickened, roughened and colored, open at the apex. Trichomes isopolar, uniformly cylindrical, up to 70 μm wide; not or slightly constricted at the cross-walls. Cells discoid, coffee bean shaped in lateral view; contents finely granular, without aerotopes; apical cells rounded, without a calyptra and thickened outer cell wall (
|
||
<figureCitation id="068873C88468FFEAFD0D4CD12CD48ABE" box="[680,745,1186,1210]" captionStart="Fig" captionStartId="2.[143,176,1087,1109]" captionTargetBox="[143,1413,259,1054]" captionTargetId="figure-406@2.[143,1413,259,1056]" captionTargetPageId="2" captionText="Fig. 2. Light micrographs of Microseira wollei collected in Gangjeong-Goryeong weir. A-D. Unbranched filament, showing thick sheath and trichomes. E, F. Discoid single cell released from filament." figureDoi="http://doi.org/10.5281/zenodo.13140639" httpUri="https://zenodo.org/record/13140639/files/figure.png" pageId="5" pageNumber="215">Fig. 2</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<caption id="CACC3FC58468FFEAFF0B4B862C358A5F" ID-DOI="http://doi.org/10.5281/zenodo.13140645" ID-Zenodo-Dep="13140645" httpUri="https://zenodo.org/record/13140645/files/figure.png" pageId="5" pageNumber="215" startId="5.[174,207,1013,1035]" targetBox="[475,1162,267,980]" targetPageId="5" targetType="figure">
|
||
<paragraph id="9E0C6F4D8468FFEAFF0B4B862C358A5F" blockId="5.[174,1445,1013,1115]" pageId="5" pageNumber="215">
|
||
<emphasis id="ACC7B35F8468FFEAFF0B4B862ED78A0F" bold="true" box="[174,234,1013,1035]" pageId="5" pageNumber="215">Fig. 5.</emphasis>
|
||
Phylogenetic tree based on the
|
||
<emphasis id="ACC7B35F8468FFEAFDA84B862C088A0E" box="[525,565,1013,1034]" italics="true" pageId="5" pageNumber="215">nifH</emphasis>
|
||
gene partial sequences aligned 318 bp showing the genetic position of
|
||
<taxonomicName id="59B314CE8468FFEAFB654B852B5C8A0E" authorityName="G. B. McGregor and Sendall ex Kennis" authorityYear="2017" baseAuthorityName="Farlow ex Gomont" box="[1216,1377,1013,1035]" class="Cyanobacteriia" family="Aerosakkonemataceae" genus="Microseira" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Cyanobacteriales" pageId="5" pageNumber="215" phylum="Cyanobacteria" rank="species" species="wollei">
|
||
<emphasis id="ACC7B35F8468FFEAFB654B852B5C8A0E" box="[1216,1377,1013,1035]" italics="true" pageId="5" pageNumber="215">Microseira wollei</emphasis>
|
||
</taxonomicName>
|
||
of Korea along with other geographical populations. Numbers indicate bootstrap value (>50%) from 1,000 replicates maximum parsimony (MP), maximum likelihood (ML), neighbor-joining (NJ) analysis respectively. GenBank accession numbers are shown in parentheses (scale bar: 0.05 nucleotide substitutions per site).
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="9E0C6F4D8468FFEAFF0B4C902D3E8AFE" blockId="5.[174,771,1250,1306]" box="[174,771,1250,1275]" pageId="5" pageNumber="215">
|
||
<heading id="C544D8218468FFEAFF0B4C902D3E8AFE" bold="true" box="[174,771,1250,1275]" centered="true" fontSize="10" level="2" pageId="5" pageNumber="215" reason="4">
|
||
<emphasis id="ACC7B35F8468FFEAFF0B4C902D3E8AFE" bold="true" box="[174,771,1250,1275]" pageId="5" pageNumber="215">Ultrastructures by transmission electron microscopy</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="9E0C6F4D8468FFEAFF0B4D712EC18B1E" blockId="5.[174,771,1250,1306]" box="[174,252,1282,1306]" pageId="5" pageNumber="215">
|
||
<emphasis id="ACC7B35F8468FFEAFF0B4D712EC18B1E" bold="true" box="[174,252,1282,1306]" pageId="5" pageNumber="215">(TEM)</emphasis>
|
||
</paragraph>
|
||
<paragraph id="9E0C6F4D8468FFEAFF604D472C3D884A" blockId="5.[174,779,1331,1614]" pageId="5" pageNumber="215">
|
||
Filaments of
|
||
<taxonomicName id="59B314CE8468FFEAFEF84D472FF58B4F" authorityName="G. B. McGregor and Sendall ex Kennis" authorityYear="2017" baseAuthorityName="Farlow ex Gomont" box="[349,456,1331,1355]" class="Cyanobacteriia" family="Aerosakkonemataceae" genus="Microseira" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Cyanobacteriales" pageId="5" pageNumber="215" phylum="Cyanobacteria" rank="species" species="wollei">
|
||
<emphasis id="ACC7B35F8468FFEAFEF84D472FF58B4F" box="[349,456,1331,1355]" italics="true" pageId="5" pageNumber="215">M. wollei</emphasis>
|
||
</taxonomicName>
|
||
were cross-sectioned to observe their ultrastructural patterns of thylakoids and intracellular contents by TEM. The trichome filaments were covered by cell wall and outer thick sheath (
|
||
<figureCitation id="068873C88468FFEAFD7B4DE72EEF8BC9" captionStart="Fig" captionStartId="3.[174,207,1266,1288]" captionTargetBox="[318,1287,257,1232]" captionTargetId="figure-244@3.[318,1287,256,1232]" captionTargetPageId="3" captionText="Fig. 3. Ultrastructures of Microseira wollei by transmission electron microscopy (TEM). A. Cross-section of filament surrounded by cell wall and thickened sheath. B. Cytoplasm filled with numerous thylakoids. C. Densely stacked thylakoids. D. Magnified thylakoids. cp (cytoplasm), cw (cell wall), sh (sheath), th(thylakoid)." figureDoi="http://doi.org/10.5281/zenodo.13140641" httpUri="https://zenodo.org/record/13140641/files/figure.png" pageId="5" pageNumber="215">Fig. 3A</figureCitation>
|
||
). The cytoplasm was filled with numerous thylakoids (
|
||
<figureCitation id="068873C88468FFEAFF134DA62F318BE9" box="[182,268,1493,1517]" captionStart="Fig" captionStartId="3.[174,207,1266,1288]" captionTargetBox="[318,1287,257,1232]" captionTargetId="figure-244@3.[318,1287,256,1232]" captionTargetPageId="3" captionText="Fig. 3. Ultrastructures of Microseira wollei by transmission electron microscopy (TEM). A. Cross-section of filament surrounded by cell wall and thickened sheath. B. Cytoplasm filled with numerous thylakoids. C. Densely stacked thylakoids. D. Magnified thylakoids. cp (cytoplasm), cw (cell wall), sh (sheath), th(thylakoid)." figureDoi="http://doi.org/10.5281/zenodo.13140641" httpUri="https://zenodo.org/record/13140641/files/figure.png" pageId="5" pageNumber="215">Fig. 3B</figureCitation>
|
||
). Membranous thylakoids were extended from near cell membrane and densely stacked as bundle (
|
||
<figureCitation id="068873C88468FFEAFD7A4D862EC9882A" captionStart="Fig" captionStartId="3.[174,207,1266,1288]" captionTargetBox="[318,1287,257,1232]" captionTargetId="figure-244@3.[318,1287,256,1232]" captionTargetPageId="3" captionText="Fig. 3. Ultrastructures of Microseira wollei by transmission electron microscopy (TEM). A. Cross-section of filament surrounded by cell wall and thickened sheath. B. Cytoplasm filled with numerous thylakoids. C. Densely stacked thylakoids. D. Magnified thylakoids. cp (cytoplasm), cw (cell wall), sh (sheath), th(thylakoid)." figureDoi="http://doi.org/10.5281/zenodo.13140641" httpUri="https://zenodo.org/record/13140641/files/figure.png" pageId="5" pageNumber="215">Fig. 3C, D</figureCitation>
|
||
). The bundles of thylakoids were located irregularly, not parietally nor radially.
|
||
</paragraph>
|
||
<paragraph id="9E0C6F4D8468FFEAFF0B4E042FA3888B" blockId="5.[174,414,1655,1679]" box="[174,414,1655,1679]" pageId="5" pageNumber="215">
|
||
<heading id="C544D8218468FFEAFF0B4E042FA3888B" bold="true" box="[174,414,1655,1679]" fontSize="10" level="2" pageId="5" pageNumber="215" reason="4">
|
||
<emphasis id="ACC7B35F8468FFEAFF0B4E042FA3888B" bold="true" box="[174,414,1655,1679]" pageId="5" pageNumber="215">Molecular phylogeny</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="9E0C6F4D8468FFEAFF604EDB2DB38B5F" blockId="5.[174,779,1703,1986]" lastBlockId="5.[839,1445,1186,1468]" pageId="5" pageNumber="215">
|
||
A total of 1,267 bp of the 16S rRNA gene and 318 bp
|
||
<emphasis id="ACC7B35F8468FFEAFF0B4EBB2EE388E4" box="[174,222,1736,1760]" italics="true" pageId="5" pageNumber="215">nifH</emphasis>
|
||
(nitrogenase iron protein) gene sequences of were analyzed. All sequences of eight isolates from
|
||
<collectingCountry id="E6A42FDD8468FFEAFD624E9B2D3788FB" box="[711,778,1768,1791]" name="South Korea" pageId="5" pageNumber="215">Korea</collectingCountry>
|
||
were identical based on these two genes. Among 1,267 bp the 16S rRNA gene sequences, sequences of the Korean population were different from 0.02% (2 bp) to the Japanese population (NIES-4236), 1.2
|
||
<emphasis id="ACC7B35F8468FFEAFDE14F1E2C6E8985" bold="true" box="[580,595,1901,1921]" pageId="5" pageNumber="215">-</emphasis>
|
||
1.3% to the Australian population, and 2.5
|
||
<emphasis id="ACC7B35F8468FFEAFE634FFE2FE889A5" bold="true" box="[454,469,1933,1953]" pageId="5" pageNumber="215">-</emphasis>
|
||
3.7% to
|
||
<collectingCountry id="E6A42FDD8468FFEAFD944FFA2C5989A5" box="[561,612,1929,1953]" name="United States of America" pageId="5" pageNumber="215">USA</collectingCountry>
|
||
population and other geographical populations in NCBI database. While, nucleotides of
|
||
<emphasis id="ACC7B35F8468FFEAFC474CD12A2C8ABE" box="[994,1041,1186,1210]" italics="true" pageId="5" pageNumber="215">nifH</emphasis>
|
||
gene showed distance as 8.4
|
||
<emphasis id="ACC7B35F8468FFEAFAE64CD52B6F8ABE" bold="true" box="[1347,1362,1190,1210]" pageId="5" pageNumber="215">-</emphasis>
|
||
8.7% to Australian samples and 3.5
|
||
<emphasis id="ACC7B35F8468FFEAFBD74CB52ABC8ADE" bold="true" box="[1138,1153,1222,1242]" pageId="5" pageNumber="215">-</emphasis>
|
||
3.8% to other populations. The neighbor joining trees are presented for 16S rRNA gene (
|
||
<figureCitation id="068873C88468FFEAFC234D712DF58B1F" box="[902,968,1282,1307]" captionStart="Fig" captionStartId="4.[145,178,1372,1394]" captionTargetBox="[253,1285,261,1339]" captionTargetId="figure-228@4.[232,1318,234,1349]" captionTargetPageId="4" captionText="Fig. 4.Phylogenetic tree based on the 16S rRNA gene partial sequences aligned 1,267 bp showing the genetic position of Microseira wollei of Korea along with other geographical populations and related taxa with some taxonomic treatment of M. wollei. Numbers indicate bootstrap value (>50%) from 1,000 replicates maximum parsimony (MP), maximum likelihood (ML), neighbor-joining (NJ) analysis respectively. GenBank accession numbers are shown in parentheses (scale bar: 0.02 nucleotide substitutions per site)." figureDoi="http://doi.org/10.5281/zenodo.13140643" httpUri="https://zenodo.org/record/13140643/files/figure.png" pageId="5" pageNumber="215">Fig. 4</figureCitation>
|
||
) and
|
||
<emphasis id="ACC7B35F8468FFEAFBA24D712A0B8B1E" box="[1031,1078,1282,1306]" italics="true" pageId="5" pageNumber="215">nifH</emphasis>
|
||
gene (
|
||
<figureCitation id="068873C88468FFEAFB254D712AFF8B1E" box="[1152,1218,1282,1306]" captionStart="Fig" captionStartId="5.[174,207,1013,1035]" captionTargetBox="[475,1162,267,980]" captionTargetId="figure-386@5.[442,1183,236,1028]" captionTargetPageId="5" captionText="Fig. 5. Phylogenetic tree based on the nifH gene partial sequences aligned 318 bp showing the genetic position of Microseira wollei of Korea along with other geographical populations. Numbers indicate bootstrap value(>50%) from 1,000 replicates maximum parsimony(MP), maximum likelihood (ML), neighbor-joining (NJ) analysis respectively. GenBank accession numbers are shown in parentheses (scale bar: 0.05 nucleotide substitutions per site)." figureDoi="http://doi.org/10.5281/zenodo.13140645" httpUri="https://zenodo.org/record/13140645/files/figure.png" pageId="5" pageNumber="215">Fig. 5</figureCitation>
|
||
). All trees supported our conspecific speculation of the Korean population of
|
||
<taxonomicName id="59B314CE8468FFEAFA224D502DBA8B5F" authorityName="G. B. McGregor and Sendall ex Kennis" authorityYear="2017" baseAuthorityName="Farlow ex Gomont" class="Cyanobacteriia" family="Aerosakkonemataceae" genus="Microseira" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Cyanobacteriales" pageId="5" pageNumber="215" phylum="Cyanobacteria" rank="species" species="wollei">
|
||
<emphasis id="ACC7B35F8468FFEAFA224D502DBA8B5F" italics="true" pageId="5" pageNumber="215">M. wollei</emphasis>
|
||
</taxonomicName>
|
||
.
|
||
</paragraph>
|
||
<paragraph id="9E0C6F4D8468FFEAFCFB4D102AAC8BB8" blockId="5.[839,1445,1186,1468]" pageId="5" pageNumber="215">
|
||
Cyanotoxin analysis by PCR of
|
||
<emphasis id="ACC7B35F8468FFEAFB604D172ADA8B7F" box="[1221,1255,1380,1403]" italics="true" pageId="5" pageNumber="215">cyr</emphasis>
|
||
genes: The
|
||
<emphasis id="ACC7B35F8468FFEAFAD44D172B998B7F" box="[1393,1444,1380,1403]" italics="true" pageId="5" pageNumber="215">cyrF</emphasis>
|
||
gene of 447 bp and
|
||
<emphasis id="ACC7B35F8468FFEAFB824DF72A688B9F" box="[1063,1109,1412,1435]" italics="true" pageId="5" pageNumber="215">cyrJ</emphasis>
|
||
gene of 626
|
||
<emphasis id="ACC7B35F8468FFEAFB444DFB2ACD8B98" bold="true" box="[1249,1264,1416,1436]" pageId="5" pageNumber="215">-</emphasis>
|
||
657 bp were not positive in all Korean samples.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |