357 lines
26 KiB
XML
357 lines
26 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.94.95751" ID-Pensoft-Pub="1314-4049-94-51" ID-Pensoft-UUID="1E443200E6585C66B1D669737794361F" ModsDocID="1314-4049-94-51" checkinTime="1670952471273" checkinUser="pensoft" docAuthor="Lu, Li, Karunarathna, Samantha C., Dai, Dong-qin, Jayawardena, Ruvishika S., Suwannarach, Nakarin & Tibpromma, Saowaluck" docDate="2022" docId="6C59D4C7E1AF5732BDD602F00DCC1623" docLanguage="en" docName="MycoKeys 94: 51-71" docOrigin="MycoKeys 94" docPubDate="2022-12-12" docSource="http://dx.doi.org/10.3897/mycokeys.94.95751" docTitle="Nigrograna asexualis L. Lu & Tibpromma 2022, sp. nov." docType="treatment" docVersion="1" id="1E443200E6585C66B1D669737794361F" lastPageNumber="51" masterDocId="1E443200E6585C66B1D669737794361F" masterDocTitle="Three new species of Nigrograna (Dothideomycetes, Pleosporales) associated with Arabica coffee from Yunnan Province, China" masterLastPageNumber="71" masterPageNumber="51" pageNumber="51" updateTime="1670952471273" updateUser="pensoft">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Three new species of Nigrograna (Dothideomycetes, Pleosporales) associated with Arabica coffee from Yunnan Province, China</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Lu, Li</mods:namePart>
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<mods:affiliation>Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Karunarathna, Samantha C.</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0001-7080-0781</mods:nameIdentifier>
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<mods:affiliation>Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Dai, Dong-qin</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0001-8935-8807</mods:nameIdentifier>
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<mods:affiliation>Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Jayawardena, Ruvishika S.</mods:namePart>
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<mods:affiliation>Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Suwannarach, Nakarin</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-2653-1913</mods:nameIdentifier>
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<mods:affiliation>School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
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<mods:nameIdentifier type="email">suwan.462@gmail.com</mods:nameIdentifier>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Tibpromma, Saowaluck</mods:namePart>
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<mods:affiliation>Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China</mods:affiliation>
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<mods:nameIdentifier type="email">saowaluckfai@gmail.com</mods:nameIdentifier>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2022</mods:date>
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<mods:detail type="pubDate">
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<mods:number>2022-12-12</mods:number>
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</mods:detail>
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<mods:detail type="volume">
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<mods:number>94</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>51</mods:start>
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<mods:end>71</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.94.95751</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.94.95751</mods:identifier>
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||
<mods:identifier type="Pensoft-Pub">1314-4049-94-51</mods:identifier>
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||
<mods:identifier type="Pensoft-UUID">1E443200E6585C66B1D669737794361F</mods:identifier>
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||
</mods:mods>
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<subSection pageId="0" pageNumber="51" type="results">
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<treatment LSID="urn:lsid:plazi:treatment:6C59D4C7E1AF5732BDD602F00DCC1623" httpUri="http://treatment.plazi.org/id/6C59D4C7E1AF5732BDD602F00DCC1623" lastPageNumber="51" pageId="0" pageNumber="51">
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<subSubSection pageId="0" pageNumber="51" type="nomenclature">
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<paragraph pageId="0" pageNumber="51">
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<taxonomicName LSID="6C59D4C7-E1AF-5732-BDD6-02F00DCC1623" authority="L. Lu & Tibpromma" authorityName="L. Lu & Tibpromma" authorityYear="2022" class="Dothideomycetes" family="Biatriosporaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna asexualis" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="species" species="asexualis" status="sp. nov.">Nigrograna asexualis L. Lu & Tibpromma</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="51">sp. nov.</taxonomicNameLabel>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="51" type="description">
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<paragraph pageId="0" pageNumber="51">
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<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Nigrograna asexualis (ZHKU 22 - 0123, holotype) a, b conidiomata on the host substrate c, d vertical sections of a conidioma e peridium f, g conidiophores with phialides h conidia i culture on PDA from above and reverse. Scale bars: 100 μm (c); 50 μm (d); 15 μm (e); 30 μm (f); 20 μm (g); 10 μm (h)." figureDoi="10.3897/mycokeys.94.95751.figure4" httpUri="https://binary.pensoft.net/fig/781091" pageId="0" pageNumber="51">Fig. 4</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="51" type="etymology">
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<paragraph pageId="0" pageNumber="51">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="51">
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The species epithet '
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<taxonomicName authorityName="L. Lu & Tibpromma" authorityYear="2022" class="Dothideomycetes" family="Nigrogranaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna asexualis" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="species" species="asexualis">
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<emphasis italics="true" pageId="0" pageNumber="51">Nigrograna asexualis</emphasis>
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</taxonomicName>
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' refers to the asexual morph.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="51" type="holotype">
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<paragraph pageId="0" pageNumber="51">Holotype.</paragraph>
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<paragraph pageId="0" pageNumber="51">ZHKU 22-0123.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="51" type="description">
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<paragraph pageId="0" pageNumber="51">Description.</paragraph>
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<paragraph pageId="0" pageNumber="51">
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<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Saprobic</emphasis>
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on decaying branch of
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<taxonomicName class="Magnoliopsida" family="Rubiaceae" genus="Coffea" kingdom="Plantae" lsidName="Coffea arabica." order="Gentianales" pageId="0" pageNumber="51" phylum="Tracheophyta" rank="species" species="arabica.">
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<emphasis italics="true" pageId="0" pageNumber="51">Coffea arabica.</emphasis>
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</taxonomicName>
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<emphasis bold="true" pageId="0" pageNumber="51">Sexual morph</emphasis>
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: Undetermined.
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<emphasis bold="true" pageId="0" pageNumber="51">Asexual morph</emphasis>
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: Coelomycetous.
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<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Pycnidia</emphasis>
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100-230
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<normalizedToken originalValue="µm">µm</normalizedToken>
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high, 120-180
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<normalizedToken originalValue="µm">µm</normalizedToken>
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wide (x̄ = 156
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<normalizedToken originalValue="×">x</normalizedToken>
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144
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, n = 10), globose to subglobose, or pyriform, immersed, solitary, unilocular, dark brown, papillate ostiole, appearing as black spots on host surface.
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<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Pycnidial wall</emphasis>
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11-16
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<normalizedToken originalValue="µm">µm</normalizedToken>
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wide (x̄ = 14
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, n = 15), brown (#937463), the wall with pseudoparenchymatous cells.
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<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Conidiophores</emphasis>
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arising from the pycnidial wall, up to 46
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<normalizedToken originalValue="µm">µm</normalizedToken>
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long and 3-4.4
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<normalizedToken originalValue="µm">µm</normalizedToken>
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wide (x̄ = 3.4
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, n = 25), filiform, septate, hyaline, simple to sparsely branched, with pegs along one or two sides and solitary phialides terminally.
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<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Phialides</emphasis>
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3-6
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<normalizedToken originalValue="×">x</normalizedToken>
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1-2
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<normalizedToken originalValue="µm">µm</normalizedToken>
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(x̄ = 4.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.5
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, n = 15), variable in shape, phialidic, discrete, ampulliform-lageniform-subcylindrical.
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<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Conidia</emphasis>
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5-6.5
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<normalizedToken originalValue="×">x</normalizedToken>
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3-4
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<normalizedToken originalValue="µm">µm</normalizedToken>
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(x̄ = 5.5
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<normalizedToken originalValue="×">x</normalizedToken>
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3.7
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, n = 30), ellipsoidal, unicellular, aseptate with 1-2 granules, subhyaline, smooth-walled.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="51" type="description">
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<paragraph pageId="0" pageNumber="51">Culture characteristics.</paragraph>
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<paragraph pageId="0" pageNumber="51">Conidium germinated on PDA within 24 h. Colonies growing on PDA reaching 5 cm diam. after two months at room temperature (22-26 °C). Colony dense, circular, surface sparsely hairy, radially striate, with a fimbriate edge, yellowish (#eabf83) to pale brown (#e1af33) at the center and dark brown (#6e5031) at the margin, reverse dark brown (#6e5031).</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="51" type="materials_examined">
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<paragraph pageId="0" pageNumber="51">Material examined.</paragraph>
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<paragraph pageId="0" pageNumber="51">
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<materialsCitation accessionNumber="OP450965, OP450971, OP432241, OP450979, OP432245, OP450966, OP450972, OP432242, OP450980, OP432246" collectingDate="2021-09-16" country="China" elevation="1016" latitude="22.600555" location="Pu'er City" longLatPrecision="21" longitude="101.01639" specimenCount="1" stateProvince="Yunnan Province" typeStatus="holotype">
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<location LSID="urn:lsid:plazi:treatment:6C59D4C7E1AF5732BDD602F00DCC1623:715A2A6D87F06CDBCCCBA3C58A5FA55E" country="China" latitude="22.600555" longLatPrecision="21" longitude="101.01639" name="Pu'er City" stateProvince="Yunnan Province">
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<normalizedToken originalValue="Pu’er">Pu'er</normalizedToken>
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City
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</location>
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,
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<collectingRegion country="China" name="Yunnan">Yunnan Province</collectingRegion>
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,
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<collectingCountry name="China">China</collectingCountry>
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, on a decaying branch of
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<taxonomicName class="Magnoliopsida" family="Rubiaceae" genus="Coffea" kingdom="Plantae" lsidName="Coffea arabica" order="Gentianales" pageId="0" pageNumber="51" phylum="Tracheophyta" rank="species" species="arabica">
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<emphasis italics="true" pageId="0" pageNumber="51">Coffea arabica</emphasis>
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</taxonomicName>
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, (
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<geoCoordinate degrees="22" direction="north" minutes="36" orientation="latitude" precision="15" seconds="2" value="22.600555">22°36'2"N</geoCoordinate>
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,
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<geoCoordinate degrees="101" direction="east" minutes="0" orientation="longitude" precision="15" seconds="59" value="101.01639">101°0'59"E</geoCoordinate>
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,
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<quantity metricMagnitude="3" metricUnit="m" metricValue="1.01643" unit="m" value="1016.43">
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<elevation metricMagnitude="3" metricUnit="m" metricValue="1.01643" unit="m" value="1016.43">1016.43 m</elevation>
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</quantity>
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),
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<collectingDate value="2021-09-16">16 September 2021</collectingDate>
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, LiLu, Puer 1-14 (ZHKU 22-0123,
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<typeStatus>holotype</typeStatus>
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), ZHKUCC 22-0214 = ZHKUCC 22-0215. GenBank number; ITS:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450965">OP450965</accessionNumber>
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, LSU:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450971">OP450971</accessionNumber>
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,
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<emphasis italics="true" pageId="0" pageNumber="51">rpb</emphasis>
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2:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP432241">OP432241</accessionNumber>
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, SSU:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450979">OP450979</accessionNumber>
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,
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<emphasis italics="true" pageId="0" pageNumber="51">tef</emphasis>
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1-α:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP432245">OP432245</accessionNumber>
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(ZHKUCC 22-0214, ex-type); ITS:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450966">OP450966</accessionNumber>
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, LSU:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450972">OP450972</accessionNumber>
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,
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<emphasis italics="true" pageId="0" pageNumber="51">rpb</emphasis>
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2:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP432242">OP432242</accessionNumber>
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, SSU:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450980">OP450980</accessionNumber>
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,
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<emphasis italics="true" pageId="0" pageNumber="51">tef</emphasis>
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1-α:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP432246">OP432246</accessionNumber>
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(ZHKUCC 22-0215)
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</materialsCitation>
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.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="51" type="notes">
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<paragraph pageId="0" pageNumber="51">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="51">
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In multi-gene phylogeny,
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<taxonomicName authorityName="L. Lu & Tibpromma" authorityYear="2022" class="Dothideomycetes" family="Nigrogranaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna asexualis" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="species" species="asexualis">
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<emphasis italics="true" pageId="0" pageNumber="51">Nigrograna asexualis</emphasis>
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</taxonomicName>
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formed a separate (68% ML, 0.97 BIPP) and distinct clade within
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<taxonomicName authorityName="J.de Gruyter, G.J.M.Verkley & P.W.Crous" authorityYear="2012" class="Dothideomycetes" family="Nigrogranaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="51">Nigrograna</emphasis>
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</taxonomicName>
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(Fig.
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<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. The maximum-likelihood phylogram of Nigrograna based on a combined SSU, LSU, ITS, rpb 2 and tef 1 - α sequence dataset with Cyclothyriella rubronotata CBS 141486 and CBS 419.85 as the outgroup taxa (Dayarathne et al. 2020). The maximum-likelihood bootstrap values (ML ≥ 60 %, left) and Bayesian Inference Posterior Probability values (BIPP ≥ 0.90, right) are shown above the nodes. Strains derived from the current study are in blue, while type strains are in bold." figureDoi="10.3897/mycokeys.94.95751.figure1" httpUri="https://binary.pensoft.net/fig/781088" pageId="0" pageNumber="51">1</figureCitation>
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). Morphologically,
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<taxonomicName lsidName="N. asexualis" pageId="0" pageNumber="51" rank="species" species="asexualis">
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<emphasis italics="true" pageId="0" pageNumber="51">N. asexualis</emphasis>
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</taxonomicName>
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conforms to the morphological characteristics of
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<taxonomicName authorityName="J.de Gruyter, G.J.M.Verkley & P.W.Crous" authorityYear="2012" class="Dothideomycetes" family="Nigrogranaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="51">Nigrograna</emphasis>
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</taxonomicName>
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by having hyaline or subhyaline, long and branched conidiophores, solitary phialides, and aseptate, ellipsoidal or cylindrical conidia (
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<bibRefCitation DOI="https://doi.org/10.1016/j.simyco.2016.09.002" author="Jaklitsch, WM" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="51" pagination="35 - 64" refId="B19" refString="Jaklitsch, WM, Voglmayr, H, 2016. Hidden diversity in Thyridaria and a new circumscription of the Thyridariaceae. Studies in Mycology 85 (1): 35 - 64, DOI: https://doi.org/10.1016/j.simyco.2016.09.002" title="Hidden diversity in Thyridaria and a new circumscription of the Thyridariaceae." url="https://doi.org/10.1016/j.simyco.2016.09.002" volume="85" year="2016">Jaklitsch and Voglmayr 2016</bibRefCitation>
|
||
;
|
||
<bibRefCitation DOI="https://doi.org/10.5943/mycosphere/11/1/1" author="Dayarathne, MC" journalOrPublisher="Mycosphere" pageId="0" pageNumber="51" pagination="1 - 188" refId="B7" refString="Dayarathne, MC, Jones, E, Maharachchikumbura, S, Devadatha, B, Sarma, V, Khongphinitbunjong, K, Chomnunti, P, Hyde, KD, 2020. Morpho-molecular characterization of microfungi associated with marine based habitats. Mycosphere 11: 1 - 188, DOI: https://doi.org/10.5943/mycosphere/11/1/1" title="Morpho-molecular characterization of microfungi associated with marine based habitats." url="https://doi.org/10.5943/mycosphere/11/1/1" volume="11" year="2020">Dayarathne et al. 2020</bibRefCitation>
|
||
;
|
||
<bibRefCitation DOI="https://doi.org/10.1371/journal.pone.0235855" author="Wanasinghe, DN" journalOrPublisher="Journal of Bacteriology" pageId="0" pageNumber="51" refId="B49" refString="Wanasinghe, DN, Wijayawardene, NN, Xu, J, Cheewangkoon, R, Mortimer, PE, 2020. Taxonomic novelties in Magnolia-associated pleosporalean fungi in the Kunming Botanical Gardens (Yunnan, China). PLoS ONE 15(7): e0235855. https://doi.org/10.1371/journal.pone.0235855" title="Taxonomic novelties in Magnolia-associated pleosporalean fungi in the Kunming Botanical Gardens (Yunnan, China). PLoS ONE 15 (7): e 0235855." url="https://doi.org/10.1371/journal.pone.0235855" year="2020">Wanasinghe et al. 2020</bibRefCitation>
|
||
). Blast results of the sequences show that ITS is similar to
|
||
<taxonomicName lsidName="N. fuscidula" pageId="0" pageNumber="51" rank="species" species="fuscidula">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">N. fuscidula</emphasis>
|
||
</taxonomicName>
|
||
with 89% (MH856004), and SSU is similar to
|
||
<taxonomicName lsidName="N. mycophila" pageId="0" pageNumber="51" rank="species" species="mycophila">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">N. mycophila</emphasis>
|
||
</taxonomicName>
|
||
with 99.8% (KX650510).
|
||
<taxonomicName authorityName="L. Lu & Tibpromma" authorityYear="2022" class="Dothideomycetes" family="Nigrogranaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna asexualis" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="species" species="asexualis">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">Nigrograna asexualis</emphasis>
|
||
</taxonomicName>
|
||
is different from
|
||
<taxonomicName lsidName="N. fuscidula" pageId="0" pageNumber="51" rank="species" species="fuscidula">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">N. fuscidula</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName lsidName="N. mycophila" pageId="0" pageNumber="51" rank="species" species="mycophila">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">N. mycophila</emphasis>
|
||
</taxonomicName>
|
||
by its ellipsoidal conidia, but the similarities of these three species are hyaline, 1-celled, smooth-walled conidia forming on philipides (
|
||
<bibRefCitation DOI="https://doi.org/10.1016/j.simyco.2016.09.002" author="Jaklitsch, WM" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="51" pagination="35 - 64" refId="B19" refString="Jaklitsch, WM, Voglmayr, H, 2016. Hidden diversity in Thyridaria and a new circumscription of the Thyridariaceae. Studies in Mycology 85 (1): 35 - 64, DOI: https://doi.org/10.1016/j.simyco.2016.09.002" title="Hidden diversity in Thyridaria and a new circumscription of the Thyridariaceae." url="https://doi.org/10.1016/j.simyco.2016.09.002" volume="85" year="2016">Jaklitsch and Voglmayr 2016</bibRefCitation>
|
||
). The LSU and
|
||
<emphasis italics="true" pageId="0" pageNumber="51">rpb</emphasis>
|
||
2 sequences of our strain blast results are similar to
|
||
<taxonomicName lsidName="N. obliqua" pageId="0" pageNumber="51" rank="species" species="obliqua">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">N. obliqua</emphasis>
|
||
</taxonomicName>
|
||
, and the similarities are 98.9% (KX650560) and 87% (KX650579) respectively, but
|
||
<taxonomicName lsidName="N. obliqua" pageId="0" pageNumber="51" rank="species" species="obliqua">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">N. obliqua</emphasis>
|
||
</taxonomicName>
|
||
lacks the asexual morph (
|
||
<bibRefCitation DOI="https://doi.org/10.1016/j.simyco.2016.09.002" author="Jaklitsch, WM" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="51" pagination="35 - 64" refId="B19" refString="Jaklitsch, WM, Voglmayr, H, 2016. Hidden diversity in Thyridaria and a new circumscription of the Thyridariaceae. Studies in Mycology 85 (1): 35 - 64, DOI: https://doi.org/10.1016/j.simyco.2016.09.002" title="Hidden diversity in Thyridaria and a new circumscription of the Thyridariaceae." url="https://doi.org/10.1016/j.simyco.2016.09.002" volume="85" year="2016">Jaklitsch and Voglmayr 2016</bibRefCitation>
|
||
). The
|
||
<emphasis italics="true" pageId="0" pageNumber="51">tef</emphasis>
|
||
1-α sequence of our strain is 95.8% (MF939615) similar to
|
||
<taxonomicName lsidName="N. locuta-pollinis" pageId="0" pageNumber="51" rank="species" species="locuta-pollinis">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">N. locuta-pollinis</emphasis>
|
||
</taxonomicName>
|
||
, which was isolated from hive-stored pollen of
|
||
<taxonomicName class="Magnoliopsida" family="Brassicaceae" genus="Brassica" kingdom="Plantae" lsidName="Brassica campestris" order="Brassicales" pageId="0" pageNumber="51" phylum="Tracheophyta" rank="species" species="campestris">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">Brassica campestris</emphasis>
|
||
</taxonomicName>
|
||
L. that lacks morphology (
|
||
<bibRefCitation DOI="https://doi.org/10.5943/mycosphere/9/6/3" author="Zhao, YZ" journalOrPublisher="Mycosphere" pageId="0" pageNumber="51" pagination="1089 - 1116" refId="B57" refString="Zhao, YZ, Zhang, ZF, Cai, L, Peng, JW, Liu, F, 2018. Four new filamentous fungal species from newly-collected and hive stored bee pollen. Mycosphere 9: 1089 - 1116, DOI: https://doi.org/10.5943/mycosphere/9/6/3" title="Four new filamentous fungal species from newly-collected and hive stored bee pollen." url="https://doi.org/10.5943/mycosphere/9/6/3" volume="9" year="2018">Zhao et al. 2018</bibRefCitation>
|
||
). Therefore, we introduce
|
||
<taxonomicName lsidName="N. asexualis" pageId="0" pageNumber="51" rank="species" species="asexualis">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">N. asexualis</emphasis>
|
||
</taxonomicName>
|
||
as a distinct new species from coffee in China.
|
||
</paragraph>
|
||
<caption doi="10.3897/mycokeys.94.95751.figure4" httpUri="https://binary.pensoft.net/fig/781091" pageId="0" pageNumber="51" start="Figure 4" startId="F4">
|
||
<paragraph pageId="0" pageNumber="51">
|
||
<emphasis bold="true" pageId="0" pageNumber="51">Figure 4.</emphasis>
|
||
<taxonomicName authorityName="L. Lu & Tibpromma" authorityYear="2022" class="Dothideomycetes" family="Nigrogranaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna asexualis" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="species" species="asexualis">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">Nigrograna asexualis</emphasis>
|
||
</taxonomicName>
|
||
(ZHKU 22-0123, holotype)
|
||
<emphasis bold="true" pageId="0" pageNumber="51">a, b</emphasis>
|
||
conidiomata on the host substrate
|
||
<emphasis bold="true" pageId="0" pageNumber="51">c, d</emphasis>
|
||
vertical sections of a conidioma
|
||
<emphasis bold="true" pageId="0" pageNumber="51">e</emphasis>
|
||
peridium
|
||
<emphasis bold="true" pageId="0" pageNumber="51">f, g</emphasis>
|
||
conidiophores with phialides
|
||
<emphasis bold="true" pageId="0" pageNumber="51">h</emphasis>
|
||
conidia
|
||
<emphasis bold="true" pageId="0" pageNumber="51">i</emphasis>
|
||
culture on PDA from above and reverse. Scale bars: 100
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis bold="true" pageId="0" pageNumber="51">c</emphasis>
|
||
); 50
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis bold="true" pageId="0" pageNumber="51">d</emphasis>
|
||
); 15
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis bold="true" pageId="0" pageNumber="51">e</emphasis>
|
||
); 30
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis bold="true" pageId="0" pageNumber="51">f</emphasis>
|
||
); 20
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis bold="true" pageId="0" pageNumber="51">g</emphasis>
|
||
); 10
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis bold="true" pageId="0" pageNumber="51">h</emphasis>
|
||
).
|
||
</paragraph>
|
||
</caption>
|
||
</subSubSection>
|
||
</treatment>
|
||
</subSection>
|
||
</document> |