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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.98.101918" ID-Pensoft-Pub="1314-4049-98-87" ID-Pensoft-UUID="A4D48209585156F58E1CEB8CBA872D78" ModsDocID="1314-4049-98-87" checkinTime="1685804678569" checkinUser="pensoft" docAuthor="Lee, Wonjun, Kim, Ji Seon, Seo, Chang Wan, Lee, Jun Won, Kim, Sung Hyun, Cho, Yoonhee &amp; Lim, Young Woon" docDate="2023" docId="FA727F832FE151FB89978D74E0C931DD" docLanguage="en" docName="MycoKeys 98: 87-111" docOrigin="MycoKeys 98" docPubDate="2023-06-02" docSource="http://dx.doi.org/10.3897/mycokeys.98.101918" docTitle="Cladosporium proteacearum Prasannath, Akinsanmi &amp; R. G. Shivas 2021" docType="treatment" docVersion="1" id="A4D48209585156F58E1CEB8CBA872D78" lastPageNumber="87" masterDocId="A4D48209585156F58E1CEB8CBA872D78" masterDocTitle="Diversity of Cladosporium (Cladosporiales, Cladosporiaceae) species in marine environments and report on five new species" masterLastPageNumber="111" masterPageNumber="87" pageNumber="87" updateTime="1685804678569" updateUser="pensoft">
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<mods:title id="12A3248C8F0D8631E7E81CF38F16E499">Diversity of Cladosporium (Cladosporiales, Cladosporiaceae) species in marine environments and report on five new species</mods:title>
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<mods:namePart id="2CDFD1148C046771EF2376CA9012D1F9">Lee, Wonjun</mods:namePart>
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<mods:namePart id="4EABD9752ABEC2281C855F066E9A3E33">Kim, Ji Seon</mods:namePart>
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<mods:namePart id="EC847F31BC27F60B92517ACD248876A2">Seo, Chang Wan</mods:namePart>
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<mods:namePart id="87650EF55E59C940EB6A54616B2275AA">Lee, Jun Won</mods:namePart>
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<mods:namePart id="F441271C3A211A97C566EC756E53AFFF">Kim, Sung Hyun</mods:namePart>
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<mods:affiliation id="15F6B3D0A5FB5AD3A8979A44488768BA">School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, Republic of Korea</mods:affiliation>
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<mods:namePart id="1E5C3F0C8BD4608585079C7913D8E25B">Cho, Yoonhee</mods:namePart>
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<treatment id="FA727F832FE151FB89978D74E0C931DD" LSID="urn:lsid:plazi:treatment:FA727F832FE151FB89978D74E0C931DD" httpUri="http://treatment.plazi.org/id/FA727F832FE151FB89978D74E0C931DD" lastPageNumber="87" pageId="0" pageNumber="87">
<subSubSection id="47607FD118EE8F3FEC17E59D134E3FA8" pageId="0" pageNumber="87" type="nomenclature">
<paragraph id="B9289C0363B9A7162DD4D695D537F8C9" pageId="0" pageNumber="87">
<taxonomicName id="AC0D958A4C17A00AC577F66ABAD05049" LSID="FA727F83-2FE1-51FB-8997-8D74E0C931DD" authority="Prasannath, Akinsanmi &amp; R. G. Shivas" authorityName="Prasannath, Akinsanmi &amp; R. G. Shivas" authorityYear="2021" class="Dothideomycetes" family="Cladosporiaceae" genus="Cladosporium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Cladosporium proteacearum" order="Cladosporiales" pageId="0" pageNumber="87" phylum="Ascomycota" rank="species" species="proteacearum">Cladosporium proteacearum Prasannath, Akinsanmi &amp; R.G. Shivas</taxonomicName>
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<paragraph id="426E8027CDD225529D6ED32B9ED01011" pageId="0" pageNumber="87">
<figureCitation id="CE8DBDBE93DF8D7414E2F8E564AEF886" captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Morphological characteristics of the new and unrecorded species on PDA, MEA, OA, and SNA media A Cladosporium lagenariiforme sp. nov. B Cladosporium maltirimosum sp. nov. C Cladosporium marinisedimentum sp. nov. D Cladosporium marinum sp. nov. E Cladosporium proteacearum F Cladosporium snafimbriatum sp. nov. &quot; F &quot; and &quot; R &quot; represent the front and the reverse sides of the culture plate, respectively. PDA: potato dextrose agar; MEA: malt extract agar; OA: oatmeal agar; SNA: synthetic nutrient-poor agar." figureDoi="10.3897/mycokeys.98.101918.figure2" httpUri="https://binary.pensoft.net/fig/858783" pageId="0" pageNumber="87">Figs 2E</figureCitation>
<figureCitation id="44AADBBC09030D5F637FD7AAADC584C0" captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Morphological characteristics of conidial structures on SNA A Cladosporium lagenariiforme sp. nov. B Cladosporium maltirimosum sp. nov. C Cladosporium marinisedimentum sp. nov. D Cladosporium marinum sp. nov. E Cladosporium proteacearum F Cladosporium snafimbriatum sp. nov. Scale bars: 10 μm (A-F)." figureDoi="10.3897/mycokeys.98.101918.figure3" httpUri="https://binary.pensoft.net/fig/858784" pageId="0" pageNumber="87">, 3E</figureCitation>
</paragraph>
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<subSubSection id="4D484631A2E62F3D2F999F4D2A8AE985" pageId="0" pageNumber="87" type="holotype">
<paragraph id="85C7FD31E48DC63315036E0D910B92A7" pageId="0" pageNumber="87">Holotype.</paragraph>
<paragraph id="F2E3E43A2CF5A64176946B985FE65702" pageId="0" pageNumber="87">
BRIP 72301a (
<bibRefCitation id="8CD0EE693B82CB2BEB7DAE3028684247" DOI="https://doi.org/10.3390/jof7110898" author="Prasannath, K" journalOrPublisher="Journal of Fungi (Basel, Switzerland)" pageId="0" pageNumber="87" pagination=": 898" refId="B40" refString="Prasannath, K, Shivas, RG, Galea, VJ, Akinsanmi, OA, 2021. Novel Botrytis and Cladosporium species associated with flower diseases of Macadamia in Australia. Journal of Fungi (Basel, Switzerland) 7 (11): 898, DOI: https://doi.org/10.3390/jof7110898" title="Novel Botrytis and Cladosporium species associated with flower diseases of Macadamia in Australia." url="https://doi.org/10.3390/jof7110898" volume="7" year="2021">Prasannath et al. 2021</bibRefCitation>
).
</paragraph>
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<subSubSection id="337F7532DBB670B00046DC9C782A836A" pageId="0" pageNumber="87" type="description">
<paragraph id="FCC728ADC7F3B7D54FD6A9FE559DC6B2" pageId="0" pageNumber="87">Description.</paragraph>
<paragraph id="7D9726AC346B5D5F5C310AF578566E8D" pageId="0" pageNumber="87">
<emphasis id="06596C2EBC99B5C319D1CDA302FF92AD" bold="true" pageId="0" pageNumber="87">
Asexual morphology:
<emphasis id="CA3002DE9EDD9B2FD3D1108D8A2E6905" italics="true" pageId="0" pageNumber="87">Mycelium</emphasis>
</emphasis>
immersed, composed of septate, branched, pale brown, smooth to verruculose hyphae, 2-5
<normalizedToken id="3209B6495441E4359A97E5BC99896145" originalValue="μm">μm</normalizedToken>
wide.
<emphasis id="8FC502AC4B0D3A6FEA44EE5CA09CC0BA" bold="true" italics="true" pageId="0" pageNumber="87">Conidiophores</emphasis>
macronematous and micronematous, arising laterally from hyphae, septate, erect to slightly flexuous, unbranched, up to 216
<normalizedToken id="01E6D71B3F0CE533CE328DCDC545AA89" originalValue="μm">μm</normalizedToken>
long, 2-4.5
<normalizedToken id="E2E8D04E9F7C06CA3B17957BC2B5CA94" originalValue="μm">μm</normalizedToken>
wide, pale brown, verruculose.
<emphasis id="6BFF333B6E5754EED3CDF8B056DCC31B" bold="true" italics="true" pageId="0" pageNumber="87">Conidiogenous cells</emphasis>
integrated, terminal, cylindrical to subcylindrical, 18-59
<normalizedToken id="05BECD751B7A9606FFF7308DEC65B6C4" originalValue="×">x</normalizedToken>
2-4.5
<normalizedToken id="FA3D57AA0FF57A4C1646D56180A649ED" originalValue="μm">μm</normalizedToken>
, bearing up to three conidiogenous loci, slightly darkened and refractive.
<taxonomicName id="A54B483A1463502CF6E6A9D817D664F7" genus="Ramoconidia" lsidName="Ramoconidia" pageId="0" pageNumber="87" rank="genus">
<emphasis id="D6007832B6D30461D6FADF117BC764E0" bold="true" italics="true" pageId="0" pageNumber="87">Ramoconidia</emphasis>
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0-1(-2)-septate, subcylindrical to cylindrical, 9.2-38
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2.3-4.7
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[av. (
<normalizedToken id="703EEBD47CE131ED4CC467A71FC168C1" originalValue="±">+/-</normalizedToken>
SD)24.1 (
<normalizedToken id="45E3A59322159BF6F433135D81B1614D" originalValue="±">+/-</normalizedToken>
7.87)
<normalizedToken id="8B593C3290395E5C7D6A84847901DC48" originalValue="×">x</normalizedToken>
3.3 (
<normalizedToken id="CD7114D49982A0003D3DDC04A1A3D551" originalValue="±">+/-</normalizedToken>
0.58)], pale brown, smooth to verruculose.
<emphasis id="F0AA8381DC011AEFEB45C9AE875D343D" bold="true" italics="true" pageId="0" pageNumber="87">Conidia</emphasis>
forming branched chains, with up to eight conidia in the terminal unbranched part, septate, pale brown, smooth to verruculose, slightly darkened and refractive hila.
<emphasis id="141AD6148597E947B4E90D5485849F98" bold="true" italics="true" pageId="0" pageNumber="87">Small terminal conidia</emphasis>
aseptate, ellipsoidal, 3.2-4.8
<normalizedToken id="15834850611C90EECAAD0B42A443C84A" originalValue="×">x</normalizedToken>
1.9-2.7
<normalizedToken id="83272E018E8BA5728970CB7F509D1E3D" originalValue="μm">μm</normalizedToken>
[av. (
<normalizedToken id="F369BAC1D491804B6CD5378041FFCB99" originalValue="±">+/-</normalizedToken>
SD) 3.9 (
<normalizedToken id="BC0478CEDE2FD38098231652701C0E74" originalValue="±">+/-</normalizedToken>
0.34)
<normalizedToken id="0864F3A3AD9B1FB03CE720625BC49FD0" originalValue="×">x</normalizedToken>
2.4 (
<normalizedToken id="958B10F95A39A1EFFADBCA313C225701" originalValue="±">+/-</normalizedToken>
0.2)].
<taxonomicName id="6F2E36B1B29727E64D1A93F9C17842F4" genus="Intercalary" lsidName="Intercalary conidia" pageId="0" pageNumber="87" rank="species" species="conidia">
<emphasis id="4EF1A059DF35154F99B8DBCF2DDB6F11" bold="true" italics="true" pageId="0" pageNumber="87">Intercalary conidia</emphasis>
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0(-1)-septate, ellipsoidal, oblong-ellipsoidal, 3.4-7.6
<normalizedToken id="610FF683E40BF07910496A62AE9532B8" originalValue="×">x</normalizedToken>
2.1-3.6
<normalizedToken id="5ECDCA178411BEE0DD17E2E7F03FF75E" originalValue="μm">μm</normalizedToken>
[av. (
<normalizedToken id="7010D0587A94026C95EA4F8895E316AB" originalValue="±">+/-</normalizedToken>
SD) 4.8 (
<normalizedToken id="A6322C0CDD5D37DB88059E180BB460B9" originalValue="±">+/-</normalizedToken>
0.83)
<normalizedToken id="5217C6B4513DDBC2F9CE5EFEE41B12F8" originalValue="×">x</normalizedToken>
2.5 (
<normalizedToken id="80ECB923EB803F20872D8EF221AB35BB" originalValue="±">+/-</normalizedToken>
0.32)].
<emphasis id="58757D4EF9622128FFCE0CF0632B6B51" bold="true" italics="true" pageId="0" pageNumber="87">Secondary ramoconidia</emphasis>
0-1-septate, oblong-ellipsoidal, ellipsoidal, subcylindrical, 4.5-25.6
<normalizedToken id="4E12E10BB122C202D41A951235C1DD51" originalValue="×">x</normalizedToken>
1.9-4.5
<normalizedToken id="E1292ED24AE3263B931B32B6627ECFD8" originalValue="μm">μm</normalizedToken>
[av. (
<normalizedToken id="06929D258E8D1C90288CAC958C28453D" originalValue="±">+/-</normalizedToken>
SD) 10.2 (
<normalizedToken id="0FEFB2136F1666DDA6E907314B16F31F" originalValue="±">+/-</normalizedToken>
4.3)
<normalizedToken id="1164DFAFBE5EEA0E7B6ECA9E3CB75A85" originalValue="×">x</normalizedToken>
3 (
<normalizedToken id="9FB21873ECC5D2F9E5D3640D275B5992" originalValue="±">+/-</normalizedToken>
0.5)].
</paragraph>
<paragraph id="710D266564AD0E0E17B4A1C601A43E98" pageId="0" pageNumber="87">
<emphasis id="CC37BDAA9111958ACCBF427D620A380C" bold="true" italics="true" pageId="0" pageNumber="87">Cultural characters</emphasis>
: Colonies on PDA 28-44 mm diam after 14 d at 25 °C, olive (2F7) to grayish yellow (2B4), reverse dark gray (1F1), velvety, raised, radially furrowed, wrinkled; margin white edge, undulated or lobate; aerial mycelia sparse, without prominent exudates, sporulation profuse, crack formed. Colonies on MEA 20-40 mm diam after 14 d at 25 °C, grayish green (29D7), reverse olive brown (4F8), velvety, powdery, raised, radially furrowed, umbonate, wrinkled; margin white, slightly undulate; aerial mycelia abundantly formed in protruding area (in the center), regular, without prominent exudates, sporulation profuse. Colonies on OA 39-46 mm diam after 14 d at 25 °C, wax white (2B3) to olive (2E5), reverse concolorous, floccose, flat, sometimes wrinkled; margin yellowish white (1A2), regular; aerial mycelia formed in spot, without prominent exudates, sporulation profuse. Colonies on SNA 40-41 mm diam after 14 d at 25 °C, olive yellow (2C7) to olive (1E4), reverse olive (2F5), powdery, flat; margin hyaline, regular; aerial mycelia sparse, without prominent exudates, sporulation profuse.
</paragraph>
</subSubSection>
<subSubSection id="67C036607E2A6E052E8BC5591CA90603" pageId="0" pageNumber="87" type="habitat">
<paragraph id="0F8FB241804C48C6A92168C8B3014453" pageId="0" pageNumber="87">Habitat and distribution.</paragraph>
<paragraph id="69D751707F38AD356ED819DF0F6B443B" pageId="0" pageNumber="87">
Isolated from plant material (Racames of
<taxonomicName id="7FFD4116606F9842D23E30B136F0470A" class="Magnoliopsida" family="Proteaceae" genus="Macadamia" kingdom="Plantae" lsidName="Macadamia integrifolia" order="Proteales" pageId="0" pageNumber="87" phylum="Tracheophyta" rank="species" species="integrifolia">
<emphasis id="189D57C1A7C4FA39D1BA2E1344CD3C35" italics="true" pageId="0" pageNumber="87">Macadamia integrifolia</emphasis>
</taxonomicName>
), sea sands, and seaweeds; Australia and Eastern, Southern, and Western Korean seaside and in Republic of Korea.
</paragraph>
</subSubSection>
<subSubSection id="08642D68245849E9A3DBE1C327201F9C" pageId="0" pageNumber="87" type="additional cultures examined">
<paragraph id="0A0E46576615DBA3025B952E45039FD3" pageId="0" pageNumber="87">Additional cultures examined.</paragraph>
<paragraph id="53E59AAC37476572A4723388099DE17B" pageId="0" pageNumber="87">
Republic of Korea. Ulsan, Ulju-gun,
<geoCoordinate id="CE542A71BB42C7827BFF2A71990AC3CE" degrees="35" direction="north" minutes="23" orientation="latitude" precision="15" seconds="00" value="35.383335">35°2300″N</geoCoordinate>
,
<geoCoordinate id="9BFBC5D5898852812FE4AB314A7A0A8F" degrees="129" direction="east" minutes="20" orientation="longitude" precision="15" seconds="46" value="129.3461">129°2046″E</geoCoordinate>
, sea sand, Jan 2021, M.S. Park &amp; Y.W. Lim (SFC20230103-M55, stored in a metabolically inactive state); Jeju-do, Hallim-eup,
<geoCoordinate id="6B0182A7724F862B9CA4E408F8CDC035" degrees="33" direction="north" minutes="23" orientation="latitude" precision="15" seconds="53" value="33.398056">33°2353″N</geoCoordinate>
,
<geoCoordinate id="97B59E4BA45A791C8B3751E2E97FEBB8" degrees="126" direction="east" minutes="14" orientation="longitude" precision="15" seconds="24" value="126.24">126°1424″E</geoCoordinate>
, seaweed, Jul 2021, M.S. Park &amp; Y.W. Lim (SFC20230103-M53, stored in a metabolically inactive state); Incheon, Ganghwa-gun,
<geoCoordinate id="F1CCB103B81FC3419C11DE5A149C21E5" degrees="37" direction="north" minutes="35" orientation="latitude" precision="15" seconds="18" value="37.588333">37°3518″N</geoCoordinate>
,
<geoCoordinate id="6A91719C2BD33D0ECB0ADCE11D290403" degrees="126" direction="east" minutes="26" orientation="longitude" precision="15" seconds="33" value="126.4425">126°2633″E</geoCoordinate>
, sea sand, Jan 2021, M.S. Park &amp; Y.W. Lim (SFC20230103-M54, stored in a metabolically inactive state).
</paragraph>
</subSubSection>
<subSubSection id="5F821651A08B534FEA4802BE78316E5A" pageId="0" pageNumber="87" type="notes">
<paragraph id="B46BBB174250812EE42DEB15CFD53B6C" pageId="0" pageNumber="87">Notes.</paragraph>
<paragraph id="6245867BAF456FDAD0C7A58B34B9959E" pageId="0" pageNumber="87">
This strain grows slower and forms less flat colonies on PDA compared to the holotype colonies (70 mm diam, flat) of
<taxonomicName id="2DE056B60D6AD922D293CF72A0788EF7" lsidName="C. proteacearum" pageId="0" pageNumber="87" rank="species" species="proteacearum">
<emphasis id="49B36E206490942559F1C4CE4C33E09C" italics="true" pageId="0" pageNumber="87">C. proteacearum</emphasis>
</taxonomicName>
(
<bibRefCitation id="AC9D40994D8647B7FC4FB6B0FF3518C4" DOI="https://doi.org/10.3390/jof7110898" author="Prasannath, K" journalOrPublisher="Journal of Fungi (Basel, Switzerland)" pageId="0" pageNumber="87" pagination=": 898" refId="B40" refString="Prasannath, K, Shivas, RG, Galea, VJ, Akinsanmi, OA, 2021. Novel Botrytis and Cladosporium species associated with flower diseases of Macadamia in Australia. Journal of Fungi (Basel, Switzerland) 7 (11): 898, DOI: https://doi.org/10.3390/jof7110898" title="Novel Botrytis and Cladosporium species associated with flower diseases of Macadamia in Australia." url="https://doi.org/10.3390/jof7110898" volume="7" year="2021">Prasannath et al. 2021</bibRefCitation>
). The characteristics of this species on MEA, OA, and SNA media were unavailable because the description on these media is insufficient (
<bibRefCitation id="9B7B3482A05D511DAB7E3403F8476457" DOI="https://doi.org/10.3390/jof7110898" author="Prasannath, K" journalOrPublisher="Journal of Fungi (Basel, Switzerland)" pageId="0" pageNumber="87" pagination=": 898" refId="B40" refString="Prasannath, K, Shivas, RG, Galea, VJ, Akinsanmi, OA, 2021. Novel Botrytis and Cladosporium species associated with flower diseases of Macadamia in Australia. Journal of Fungi (Basel, Switzerland) 7 (11): 898, DOI: https://doi.org/10.3390/jof7110898" title="Novel Botrytis and Cladosporium species associated with flower diseases of Macadamia in Australia." url="https://doi.org/10.3390/jof7110898" volume="7" year="2021">Prasannath et al. 2021</bibRefCitation>
). Septa are visible in the secondary ramoconidia and intercalary conidia of this strain, but they have not been reported for the holotype (
<bibRefCitation id="0A8CEE2FAC99EAC50F74FAA3D1ED680F" DOI="https://doi.org/10.3390/jof7110898" author="Prasannath, K" journalOrPublisher="Journal of Fungi (Basel, Switzerland)" pageId="0" pageNumber="87" pagination=": 898" refId="B40" refString="Prasannath, K, Shivas, RG, Galea, VJ, Akinsanmi, OA, 2021. Novel Botrytis and Cladosporium species associated with flower diseases of Macadamia in Australia. Journal of Fungi (Basel, Switzerland) 7 (11): 898, DOI: https://doi.org/10.3390/jof7110898" title="Novel Botrytis and Cladosporium species associated with flower diseases of Macadamia in Australia." url="https://doi.org/10.3390/jof7110898" volume="7" year="2021">Prasannath et al. 2021</bibRefCitation>
). To our knowledge, this is the first report of this species from the Republic of Korea and from marine environments.
</paragraph>
</subSubSection>
</treatment>
</document>